RchiOBHm_Chr2g0167291

Protein of unknown function (DUF1997)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0003399
Physical Location & Seq
Reverse (-)
81876276 .. 81877597
1322 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ53508

Sequence Viewer

Length: 732 bp
ATGGTTGGGGCAGTTGCTTTATGCCAACCCTTGTTTATTCTTGGTACTAGTCTGCCTTTTCCAGAAAAATTGAAGGTGAAGCTCAAGAAGGGCAGACAGCGCCACTATGCGAAAGCGGTATGGAACTCAGATCATAAGAAGCCTTTTACCGTGAACCTCAGTTCCACTATTGATGCACCTCTGTATGAGTCCCCTGAGGTTTCATTTGATCAATATTTGGAGGATGATGAACAAAGGGTTATTAAAGCACTTTTTCCAGACATGGAGGACCAGCAAATTAGCAAGGAAGAGTGGAGGATTCAAATCCCAACCATGCAAGTCTTGTTCCTGTCTATCTACTTTGCAATGTACATCAAGTTAAGATTCAAATCCATGGGGAAAGACTACCCTCCTCATGTTCCTCAGCACATCGCCAAAATCACTGAGCTTGAAATTACAAGATGGGAACTTGGGGGGATCCCTGAGGAGTACCAGCCGCGTGACTTGAGCCTCTTCCTCAACGGAACCATCTATCCTGATAGAAAGCAAGGAACAAAAAGCAGACTTAGAGGTCAGTTTGACATAAACATGAGCTTTATTGTATCACCTCTGCTTGGTTGGATTCCTCATACTGTCGCGCAGAGCCTTGTAGAAGCGATGGTTAAGAAAGCGATGACGGCTTCGACCTCAAATTCCAGATTGGTAGAAGACTATGATGAATTCAAGATGGAGAAACTCAAGAAAGTTGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1997

Protein Analysis

243

Amino Acids

28.11

Weight (kDa)

8.75

Isoelectric Point (pI)

44.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1997 PF09366 69 - 234 1.8e-19 Protein of unknown function (DUF1997)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 549
AccII CGCG 2 cut(s) 478, 617
AciI CCGC 2 cut(s) 116, 476
AclWI GGATC 2 cut(s) 451, 464
AcsI RAATTY 2 cut(s) 670, 698
AfaI GTAC 3 cut(s) 46, 350, 470
AfiI CCNNNNNNNGG 1 cut(s) 593
AgsI TTSAA 5 cut(s) 73, 302, 367, 431, 703
AhlI ACTAGT 1 cut(s) 47
AloI GAACNNNNNNTCC 4 cut(s) 146, 178, 496, 528
AluBI AGCT 3 cut(s) 82, 427, 573
AluI AGCT 3 cut(s) 82, 427, 573
AlwI GGATC 2 cut(s) 451, 464
ApoI RAATTY 2 cut(s) 670, 698
AspLEI GCGC 2 cut(s) 102, 619
AspS9I GGNCC 1 cut(s) 268
AsuHPI GGTGA 2 cut(s) 88, 576
AvaII GGWCC 1 cut(s) 268
AxyI CCTNAGG 2 cut(s) 195, 462
BamHI GGATCC 1 cut(s) 456
BbsI GAAGAC 1 cut(s) 693
BbvCI CCTCAGC 1 cut(s) 402
BccI CCATC 4 cut(s) 435, 515, 631, 700
BceAI ACGGC 1 cut(s) 672
BclI TGATCA 1 cut(s) 208
BcuI ACTAGT 1 cut(s) 47
BfaI CTAG 1 cut(s) 48
BfoI RGCGCY 1 cut(s) 103
BisI GCNGC 1 cut(s) 476
BlsI GCNGC 1 cut(s) 477
Bme18I GGWCC 1 cut(s) 268
BmgT120I GGNCC 1 cut(s) 268
BmiI GGNNCC 2 cut(s) 458, 505
BmsI GCATC 1 cut(s) 163
BpiI GAAGAC 1 cut(s) 693
Bpu10I CCTNAGC 1 cut(s) 402
BpuEI CTTGAG 3 cut(s) 68, 505, 701
BsaBI GATNNNNATC 2 cut(s) 302, 367
BsaJI CCNNGG 1 cut(s) 372
Bsc4I CCNNNNNNNGG 1 cut(s) 593
Bse21I CCTNAGG 2 cut(s) 195, 462
Bse3DI GCAATG 1 cut(s) 351
Bse8I GATNNNNATC 2 cut(s) 302, 367
BseDI CCNNGG 1 cut(s) 372
BseGI GGATG 1 cut(s) 229
BseJI GATNNNNATC 2 cut(s) 302, 367
BseLI CCNNNNNNNGG 1 cut(s) 593
BseMI GCAATG 1 cut(s) 351
BseMII CTCAG 6 cut(s) 141, 172, 186, 414, 416, 453
BseRI GAGGAG 2 cut(s) 381, 479
Bsh1236I CGCG 2 cut(s) 478, 617
BslFI GGGAC 1 cut(s) 175
BslI CCNNNNNNNGG 1 cut(s) 593
BsmFI GGGAC 1 cut(s) 175
Bsp1407I TGTACA 1 cut(s) 348
Bsp143I GATC 3 cut(s) 130, 208, 456
Bsp19I CCATGG 1 cut(s) 372
BspACI CCGC 2 cut(s) 116, 476
BspCNI CTCAG 6 cut(s) 140, 171, 187, 415, 415, 454
BspFNI CGCG 2 cut(s) 478, 617
BspLI GGNNCC 2 cut(s) 458, 505
BspPI GGATC 2 cut(s) 451, 464
BsrDI GCAATG 1 cut(s) 351
BsrGI TGTACA 1 cut(s) 348
BssECI CCNNGG 1 cut(s) 372
BssMI GATC 3 cut(s) 130, 208, 456
BssT1I CCWWGG 1 cut(s) 372
Bst4CI ACNGT 2 cut(s) 151, 613
Bst6I CTCTTC 2 cut(s) 282, 497
BstAUI TGTACA 1 cut(s) 348
BstDEI CTNAG 7 cut(s) 127, 158, 195, 402, 423, 462, 545
BstDSI CCRYGG 1 cut(s) 372
BstF5I GGATG 1 cut(s) 229
BstFNI CGCG 2 cut(s) 478, 617
BstH2I RGCGCY 1 cut(s) 103
BstHHI GCGC 2 cut(s) 102, 619
BstKTI GATC 3 cut(s) 133, 211, 459
BstMBI GATC 3 cut(s) 130, 208, 456
BstMWI GCNNNNNNNGC 2 cut(s) 99, 656
BstUI CGCG 2 cut(s) 478, 617
BstV2I GAAGAC 1 cut(s) 693
BstX2I RGATCY 1 cut(s) 456
BstYI RGATCY 1 cut(s) 456
Bsu36I CCTNAGG 2 cut(s) 195, 462
BtgI CCRYGG 1 cut(s) 372
BtgZI GCGATG 3 cut(s) 394, 650, 665
BtsCI GGATG 1 cut(s) 229
BtsIMutI CAGTG 1 cut(s) 420
CfoI GCGC 2 cut(s) 102, 619
Cfr13I GGNCC 1 cut(s) 268
Csp6I GTAC 3 cut(s) 45, 349, 469
CviAII CATG 5 cut(s) 262, 313, 373, 395, 568
CviJI RGCY 8 cut(s) 82, 142, 427, 475, 489, 573, 624, 659
CviKI_1 RGCY 8 cut(s) 82, 142, 427, 475, 489, 573, 624, 659
CviQI GTAC 3 cut(s) 45, 349, 469
DdeI CTNAG 7 cut(s) 127, 158, 195, 402, 423, 462, 545
DpnI GATC 3 cut(s) 132, 210, 458
DpnII GATC 3 cut(s) 130, 208, 456
DrdI GACNNNNNNGTC 1 cut(s) 549
DseDI GACNNNNNNGTC 1 cut(s) 549
Eam1104I CTCTTC 2 cut(s) 282, 497
EarI CTCTTC 2 cut(s) 282, 497
Eco130I CCWWGG 1 cut(s) 372
Eco47I GGWCC 1 cut(s) 268
Eco81I CCTNAGG 2 cut(s) 195, 462
EcoRI GAATTC 1 cut(s) 698
EcoT14I CCWWGG 1 cut(s) 372
ErhI CCWWGG 1 cut(s) 372
FaeI CATG 5 cut(s) 265, 316, 376, 398, 571
FaqI GGGAC 1 cut(s) 175
FatI CATG 5 cut(s) 261, 312, 372, 394, 567
FbaI TGATCA 1 cut(s) 208
Fnu4HI GCNGC 1 cut(s) 476
FokI GGATG 1 cut(s) 236
Fsp4HI GCNGC 1 cut(s) 476
FspBI CTAG 1 cut(s) 48
GlaI GCGC 2 cut(s) 101, 618
GluI GCNGC 1 cut(s) 476
HaeII RGCGCY 1 cut(s) 103
HhaI GCGC 2 cut(s) 102, 619
Hin1II CATG 5 cut(s) 265, 316, 376, 398, 571
Hin6I GCGC 2 cut(s) 100, 617
HinP1I GCGC 2 cut(s) 100, 617
HinfI GANTC 4 cut(s) 188, 298, 363, 601
HphI GGTGA 2 cut(s) 88, 576
Hpy166II GTNNAC 1 cut(s) 154
Hpy188I TCNGA 1 cut(s) 130
Hpy188III TCNNGA 7 cut(s) 62, 85, 257, 515, 675, 703, 718
Hpy8I GTNNAC 1 cut(s) 154
HpyAV CCTTC 2 cut(s) 67, 82
HpyCH4III ACNGT 2 cut(s) 151, 613
HpyCH4V TGCA 3 cut(s) 176, 316, 344
HpyF10VI GCNNNNNNNGC 2 cut(s) 99, 656
HpyF3I CTNAG 7 cut(s) 127, 158, 195, 402, 423, 462, 545
Hsp92II CATG 5 cut(s) 265, 316, 376, 398, 571
HspAI GCGC 2 cut(s) 100, 617
Ksp22I TGATCA 1 cut(s) 208
Kzo9I GATC 3 cut(s) 130, 208, 456
LpnPI CCDG 9 cut(s) 75, 207, 270, 284, 341, 474, 485, 528, 688
LweI GCATC 1 cut(s) 163
MaeI CTAG 1 cut(s) 48
MaeIII GTNAC 1 cut(s) 479
MalI GATC 3 cut(s) 132, 210, 458
MboI GATC 3 cut(s) 130, 208, 456
MboII GAAGA 3 cut(s) 299, 484, 698
MflI RGATCY 1 cut(s) 456
MluCI AATT 5 cut(s) 68, 276, 432, 670, 698
MlyI GAGTC 1 cut(s) 197
MmeI TCCRAC 1 cut(s) 578
MseI TTAA 3 cut(s) 243, 359, 642
MslI CAYNNNNRTG 1 cut(s) 566
MvnI CGCG 2 cut(s) 478, 617
MwoI GCNNNNNNNGC 2 cut(s) 99, 656
NcoI CCATGG 1 cut(s) 372
NdeII GATC 3 cut(s) 130, 208, 456
NlaIII CATG 5 cut(s) 265, 316, 376, 398, 571
NlaIV GGNNCC 2 cut(s) 458, 505
NmuCI GTSAC 1 cut(s) 479
PfeI GAWTC 3 cut(s) 298, 363, 601
PkrI GCNGC 1 cut(s) 477
PleI GAGTC 1 cut(s) 196
PpsI GAGTC 1 cut(s) 196
PspN4I GGNNCC 2 cut(s) 458, 505
PspPI GGNCC 1 cut(s) 268
PsuI RGATCY 1 cut(s) 456
RsaI GTAC 3 cut(s) 46, 350, 470
RsaNI GTAC 3 cut(s) 45, 349, 469
RseI CAYNNNNRTG 1 cut(s) 566
SaqAI TTAA 3 cut(s) 243, 359, 642
SatI GCNGC 1 cut(s) 476
Sau3AI GATC 3 cut(s) 130, 208, 456
Sau96I GGNCC 1 cut(s) 268
SchI GAGTC 1 cut(s) 197
SfaNI GCATC 1 cut(s) 163
SinI GGWCC 1 cut(s) 268
SmiMI CAYNNNNRTG 1 cut(s) 566
SmlI CTYRAG 3 cut(s) 83, 484, 716
SmoI CTYRAG 3 cut(s) 83, 484, 716
SpeI ACTAGT 1 cut(s) 47
Sse9I AATT 5 cut(s) 68, 276, 432, 670, 698
SsiI CCGC 2 cut(s) 116, 476
SspI AATATT 1 cut(s) 215
SspMI CTAG 1 cut(s) 48
StyI CCWWGG 1 cut(s) 372
TaaI ACNGT 2 cut(s) 151, 613
TaqI TCGA 1 cut(s) 662
TasI AATT 5 cut(s) 68, 276, 432, 670, 698
TatI WGTACW 1 cut(s) 348
TauI GCSGC 1 cut(s) 478
TfiI GAWTC 3 cut(s) 298, 363, 601
Tru1I TTAA 3 cut(s) 243, 359, 642
Tru9I TTAA 3 cut(s) 243, 359, 642
TscAI CASTG 1 cut(s) 427
TseFI GTSAC 1 cut(s) 479
Tsp45I GTSAC 1 cut(s) 479
TspDTI ATGAA 3 cut(s) 192, 243, 711
TspGWI ACGGA 1 cut(s) 516
TspRI CASTG 1 cut(s) 427
VpaK11BI GGWCC 1 cut(s) 268
XapI RAATTY 2 cut(s) 670, 698
XspI CTAG 1 cut(s) 48
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.