RLG00000021702

Protein of unknown function (DUF1997)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0003399
Physical Location & Seq
Reverse (-)
77363780 .. 77364980
1201 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000021702

Sequence Viewer

Length: 612 bp
ATGGTTGAGGCAATTGCTGTCTGCAGAATTCTTGATAGAGGTATGCCTTCTCGCCCAGAAAAGCTGAAGGTGAAGCTGAAAAAGAAGCAGCAGGACAGGCATTGCCATTATGCAAAGGCGCTATTCAACTTGCCGAAAAAGGATGACCAGATGAAACCTTTAACCTTGCACCACATCACTACTGTTGCCCTACTCTGTGAGTCCTCCAAGGCTTCATTTGATCAATATTTGAAGGATGAACAAAGGGTTATTAAAGCCCTATTTCCAGGCATAGAGGCAAGAAAACTTAACAAGAAAGAGTGGAGGATTCAAATGCCAACCATGCAATTCTTGTTCATGTCTGTCTGCCTTGTATTATACATCAGTTTAAGATACAAGTCCAAAGGGAAAGACCATCCTCCTCATGTTCCTCATCACATCCCCATGGTCCTTGAGCTTGAAGCTATTGACTTAATCTTAAGCTTCATTGTTTCACCTCTGCTTGGTTGGGTTCCACAAATTGTTATGCATAGCCTCATAAAATCGCTGGTGAAGACTGTGATGGCGGATTCGACATCAAATGCCAGATTGCTTGAAGACTATGATGTATTCAAAACAAAGAATGTGGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1997

Protein Analysis

204

Amino Acids

23.44

Weight (kDa)

9.59

Isoelectric Point (pI)

46.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1997 PF09366 73 - 129 4.4e-09 Protein of unknown function (DUF1997)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 545
AcsI RAATTY 1 cut(s) 27
AcuI CTGAAG 1 cut(s) 86
AfiI CCNNNNNNNGG 1 cut(s) 482
AflII CTTAAG 1 cut(s) 457
AgsI TTSAA 6 cut(s) 127, 232, 311, 440, 575, 592
AjnI CCWGG 1 cut(s) 265
AluBI AGCT 5 cut(s) 64, 76, 436, 443, 462
AluI AGCT 5 cut(s) 64, 76, 436, 443, 462
ApeKI GCWGC 1 cut(s) 88
ApoI RAATTY 1 cut(s) 27
AspLEI GCGC 1 cut(s) 121
AspS9I GGNCC 1 cut(s) 427
AsuHPI GGTGA 3 cut(s) 82, 465, 541
AvaII GGWCC 1 cut(s) 427
BbsI GAAGAC 2 cut(s) 539, 582
BbvI GCAGC 1 cut(s) 100
BccI CCATC 2 cut(s) 402, 535
BciT130I CCWGG 1 cut(s) 267
BclI TGATCA 1 cut(s) 220
BfmI CTRYAG 1 cut(s) 22
BfoI RGCGCY 1 cut(s) 122
BfrI CTTAAG 1 cut(s) 457
BisI GCNGC 1 cut(s) 89
BlsI GCNGC 1 cut(s) 90
Bme1390I CCNGG 1 cut(s) 267
Bme18I GGWCC 1 cut(s) 427
BmgT120I GGNCC 1 cut(s) 427
BmiI GGNNCC 1 cut(s) 492
BmrFI CCNGG 1 cut(s) 267
BpiI GAAGAC 2 cut(s) 539, 582
BpuEI CTTGAG 1 cut(s) 452
BsaJI CCNNGG 2 cut(s) 207, 423
Bsc4I CCNNNNNNNGG 1 cut(s) 482
Bse3DI GCAATG 1 cut(s) 100
BseBI CCWGG 1 cut(s) 267
BseDI CCNNGG 2 cut(s) 207, 423
BseGI GGATG 4 cut(s) 148, 241, 394, 417
BseLI CCNNNNNNNGG 1 cut(s) 482
BseMI GCAATG 1 cut(s) 100
BseRI GAGGAG 1 cut(s) 390
BseXI GCAGC 1 cut(s) 100
BslI CCNNNNNNNGG 1 cut(s) 482
Bsp143I GATC 1 cut(s) 220
Bsp19I CCATGG 1 cut(s) 423
BspACI CCGC 1 cut(s) 545
BspLI GGNNCC 1 cut(s) 492
BspMAI CTGCAG 1 cut(s) 26
BspTI CTTAAG 1 cut(s) 457
BsrDI GCAATG 1 cut(s) 100
BssECI CCNNGG 2 cut(s) 207, 423
BssMI GATC 1 cut(s) 220
BssT1I CCWWGG 2 cut(s) 207, 423
Bst2UI CCWGG 1 cut(s) 267
Bst4CI ACNGT 2 cut(s) 184, 538
BstAFI CTTAAG 1 cut(s) 457
BstDSI CCRYGG 1 cut(s) 423
BstF5I GGATG 4 cut(s) 148, 241, 394, 417
BstH2I RGCGCY 1 cut(s) 122
BstHHI GCGC 1 cut(s) 121
BstKTI GATC 1 cut(s) 223
BstMBI GATC 1 cut(s) 220
BstMWI GCNNNNNNNGC 2 cut(s) 97, 322
BstNI CCWGG 1 cut(s) 267
BstSCI CCNGG 1 cut(s) 265
BstSFI CTRYAG 1 cut(s) 22
BstV1I GCAGC 1 cut(s) 100
BstV2I GAAGAC 2 cut(s) 539, 582
BtgI CCRYGG 1 cut(s) 423
BtsCI GGATG 4 cut(s) 148, 241, 394, 417
CfoI GCGC 1 cut(s) 121
Cfr13I GGNCC 1 cut(s) 427
CspCI CAANNNNNGTGG 1 cut(s) 585
CviAII CATG 4 cut(s) 322, 337, 404, 424
CviJI RGCY 8 cut(s) 64, 76, 212, 257, 436, 443, 462, 513
CviKI_1 RGCY 8 cut(s) 64, 76, 212, 257, 436, 443, 462, 513
DpnI GATC 1 cut(s) 222
DpnII GATC 1 cut(s) 220
EciI GGCGGA 1 cut(s) 560
Eco130I CCWWGG 2 cut(s) 207, 423
Eco47I GGWCC 1 cut(s) 427
Eco57I CTGAAG 1 cut(s) 86
EcoRI GAATTC 1 cut(s) 27
EcoRII CCWGG 1 cut(s) 265
EcoT14I CCWWGG 2 cut(s) 207, 423
EcoT22I ATGCAT 1 cut(s) 510
ErhI CCWWGG 2 cut(s) 207, 423
FaeI CATG 4 cut(s) 325, 340, 407, 427
FatI CATG 4 cut(s) 321, 336, 403, 423
FbaI TGATCA 1 cut(s) 220
Fnu4HI GCNGC 1 cut(s) 89
FokI GGATG 4 cut(s) 155, 248, 381, 404
Fsp4HI GCNGC 1 cut(s) 89
GlaI GCGC 1 cut(s) 120
GluI GCNGC 1 cut(s) 89
HaeII RGCGCY 1 cut(s) 122
HhaI GCGC 1 cut(s) 121
Hin1II CATG 4 cut(s) 325, 340, 407, 427
Hin6I GCGC 1 cut(s) 119
HinP1I GCGC 1 cut(s) 119
HindIII AAGCTT 1 cut(s) 460
HinfI GANTC 3 cut(s) 200, 307, 548
HphI GGTGA 3 cut(s) 82, 465, 541
Hpy188III TCNNGA 1 cut(s) 32
HpyAV CCTTC 3 cut(s) 57, 61, 226
HpyCH4III ACNGT 2 cut(s) 184, 538
HpyCH4V TGCA 5 cut(s) 24, 113, 169, 325, 508
HpyF10VI GCNNNNNNNGC 2 cut(s) 97, 322
Hsp92II CATG 4 cut(s) 325, 340, 407, 427
HspAI GCGC 1 cut(s) 119
Ksp22I TGATCA 1 cut(s) 220
Kzo9I GATC 1 cut(s) 220
LpnPI CCDG 8 cut(s) 69, 77, 82, 161, 252, 279, 512, 577
Lsp1109I GCAGC 1 cut(s) 100
MalI GATC 1 cut(s) 222
MboI GATC 1 cut(s) 220
MboII GAAGA 2 cut(s) 544, 587
MfeI CAATTG 1 cut(s) 12
MluCI AATT 4 cut(s) 12, 27, 326, 498
MlyI GAGTC 1 cut(s) 209
MnlI CCTC 9 cut(s) 32, 214, 268, 297, 408, 411, 420, 486, 524
Mph1103I ATGCAT 1 cut(s) 510
MseI TTAA 6 cut(s) 161, 252, 288, 368, 452, 458
MslI CAYNNNNRTG 1 cut(s) 422
MspCI CTTAAG 1 cut(s) 457
MspR9I CCNGG 1 cut(s) 267
MunI CAATTG 1 cut(s) 12
MvaI CCWGG 1 cut(s) 267
MwoI GCNNNNNNNGC 2 cut(s) 97, 322
NcoI CCATGG 1 cut(s) 423
NdeII GATC 1 cut(s) 220
NlaIII CATG 4 cut(s) 325, 340, 407, 427
NlaIV GGNNCC 1 cut(s) 492
NsiI ATGCAT 1 cut(s) 510
PfeI GAWTC 2 cut(s) 307, 548
PkrI GCNGC 1 cut(s) 90
PleI GAGTC 1 cut(s) 208
PpsI GAGTC 1 cut(s) 208
Psp6I CCWGG 1 cut(s) 265
PspGI CCWGG 1 cut(s) 265
PspN4I GGNNCC 1 cut(s) 492
PspPI GGNCC 1 cut(s) 427
PstI CTGCAG 1 cut(s) 26
RseI CAYNNNNRTG 1 cut(s) 422
SaqAI TTAA 6 cut(s) 161, 252, 288, 368, 452, 458
SatI GCNGC 1 cut(s) 89
Sau3AI GATC 1 cut(s) 220
Sau96I GGNCC 1 cut(s) 427
SchI GAGTC 1 cut(s) 209
ScrFI CCNGG 1 cut(s) 267
SfcI CTRYAG 1 cut(s) 22
SinI GGWCC 1 cut(s) 427
SmiMI CAYNNNNRTG 1 cut(s) 422
SmlI CTYRAG 2 cut(s) 431, 457
SmoI CTYRAG 2 cut(s) 431, 457
Sse9I AATT 4 cut(s) 12, 27, 326, 498
SsiI CCGC 1 cut(s) 545
SspI AATATT 1 cut(s) 227
StyD4I CCNGG 1 cut(s) 265
StyI CCWWGG 2 cut(s) 207, 423
TaaI ACNGT 2 cut(s) 184, 538
TaqI TCGA 1 cut(s) 551
TasI AATT 4 cut(s) 12, 27, 326, 498
TfiI GAWTC 2 cut(s) 307, 548
Tru1I TTAA 6 cut(s) 161, 252, 288, 368, 452, 458
Tru9I TTAA 6 cut(s) 161, 252, 288, 368, 452, 458
TseI GCWGC 1 cut(s) 88
TspDTI ATGAA 5 cut(s) 167, 204, 252, 325, 454
Vha464I CTTAAG 1 cut(s) 457
VpaK11BI GGWCC 1 cut(s) 427
XapI RAATTY 1 cut(s) 27
Zsp2I ATGCAT 1 cut(s) 510
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.