Rh2DG619000

Protein of unknown function (DUF1997)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0003399
Physical Location & Seq
Reverse (-)
84883391 .. 84884538
1148 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG619000.1

Sequence Viewer

Length: 729 bp
ATGGTTGGGGCAGTTGCTTTATGCCAACCCTTGTTTATTCTTGGTACTAGTCTGCCTTTTCCAGAAAAATTGAAGGTGAAGCTGAAAAAGGGCAGACAGCGCCACTATGCGAAAGCGGTATGGAACTCAGATCATAAGAAGCCTTTTACCGTGAACCTCAGTTCCACTATTGATGCACCTCTGTATGAGTCCCCTGAGGTTTCATTTGATCAATATTTGGAGGATGAACAGAGGGTTATCAAAGCACTTTTCCCAGACATTGACGACCAGCAACTTAGCAAGGAAGAGTGGAGGATTCAAATCCCAACCATGCAAGTCTTGTTCCTGTCTATCTACTTTGCAATGTACATCAAGTTAAGATTCAAATCCATGGGGAAAGACTACCCTCCTCATGTTCCTCAGCACATCGCCAAAATCACTGAGCTTGAAATTACAAGATGGGAACTTGGGGGGATCCCTGAGGAGTACCAGCCGCGTGACTTGAGCCTCTCCCTCAACGGAACCATCTATCCTGATAGAAAGCAAGGAACAAAAAGCAGACTTAGAGGTCAGTTTGACATAAACATGAGCTTTATTGTATCACCTCTGCCTGGTTGGATTCCTCGTACTGTCGTGCAGAGCCTTGTAGAAGCGCTGGTTAAGAAAGCGATGGCGGCTTCGACCTCAAATTCCAGATTGGTAGAAGACTATGATGAATTCAAGATGGAGAAACTCAAGAATGTTGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1997

Protein Analysis

242

Amino Acids

27.88

Weight (kDa)

8.96

Isoelectric Point (pI)

45.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1997 PF09366 69 - 233 2.6e-20 Protein of unknown function (DUF1997)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 546
AccII CGCG 1 cut(s) 475
AciI CCGC 3 cut(s) 116, 473, 653
AclWI GGATC 2 cut(s) 448, 461
AcsI RAATTY 2 cut(s) 667, 695
AfaI GTAC 4 cut(s) 46, 347, 467, 607
AfeI AGCGCT 1 cut(s) 633
AfiI CCNNNNNNNGG 1 cut(s) 590
AgsI TTSAA 5 cut(s) 73, 299, 364, 428, 700
AhlI ACTAGT 1 cut(s) 47
AjnI CCWGG 1 cut(s) 589
AloI GAACNNNNNNTCC 4 cut(s) 146, 178, 493, 525
AluBI AGCT 3 cut(s) 82, 424, 570
AluI AGCT 3 cut(s) 82, 424, 570
AlwI GGATC 2 cut(s) 448, 461
Aor51HI AGCGCT 1 cut(s) 633
ApoI RAATTY 2 cut(s) 667, 695
AspLEI GCGC 2 cut(s) 102, 634
AsuHPI GGTGA 2 cut(s) 88, 573
AxyI CCTNAGG 2 cut(s) 195, 459
BamHI GGATCC 1 cut(s) 453
BbsI GAAGAC 1 cut(s) 690
BbvCI CCTCAGC 1 cut(s) 399
BccI CCATC 4 cut(s) 432, 512, 643, 697
BciT130I CCWGG 1 cut(s) 591
BclI TGATCA 1 cut(s) 208
BcuI ACTAGT 1 cut(s) 47
BfaI CTAG 1 cut(s) 48
BfoI RGCGCY 2 cut(s) 103, 635
BisI GCNGC 2 cut(s) 473, 654
BlsI GCNGC 2 cut(s) 474, 655
Bme1390I CCNGG 1 cut(s) 591
BmiI GGNNCC 2 cut(s) 455, 502
BmrFI CCNGG 1 cut(s) 591
BmsI GCATC 1 cut(s) 163
BpiI GAAGAC 1 cut(s) 690
Bpu10I CCTNAGC 1 cut(s) 399
BpuEI CTTGAG 2 cut(s) 502, 698
BsaBI GATNNNNATC 2 cut(s) 299, 364
BsaJI CCNNGG 1 cut(s) 369
Bsc4I CCNNNNNNNGG 1 cut(s) 590
Bse21I CCTNAGG 2 cut(s) 195, 459
Bse3DI GCAATG 1 cut(s) 348
Bse8I GATNNNNATC 2 cut(s) 299, 364
BseBI CCWGG 1 cut(s) 591
BseDI CCNNGG 1 cut(s) 369
BseGI GGATG 1 cut(s) 229
BseJI GATNNNNATC 2 cut(s) 299, 364
BseLI CCNNNNNNNGG 1 cut(s) 590
BseMI GCAATG 1 cut(s) 348
BseMII CTCAG 6 cut(s) 141, 172, 186, 411, 413, 450
BseRI GAGGAG 2 cut(s) 378, 476
BsgI GTGCAG 1 cut(s) 635
Bsh1236I CGCG 1 cut(s) 475
BslFI GGGAC 1 cut(s) 175
BslI CCNNNNNNNGG 1 cut(s) 590
BsmFI GGGAC 1 cut(s) 175
Bsp1407I TGTACA 1 cut(s) 345
Bsp143I GATC 3 cut(s) 130, 208, 453
Bsp19I CCATGG 1 cut(s) 369
BspACI CCGC 3 cut(s) 116, 473, 653
BspCNI CTCAG 6 cut(s) 140, 171, 187, 412, 412, 451
BspFNI CGCG 1 cut(s) 475
BspLI GGNNCC 2 cut(s) 455, 502
BspPI GGATC 2 cut(s) 448, 461
BsrDI GCAATG 1 cut(s) 348
BsrGI TGTACA 1 cut(s) 345
BssECI CCNNGG 1 cut(s) 369
BssMI GATC 3 cut(s) 130, 208, 453
BssT1I CCWWGG 1 cut(s) 369
Bst2UI CCWGG 1 cut(s) 591
Bst4CI ACNGT 2 cut(s) 151, 610
Bst6I CTCTTC 1 cut(s) 279
BstAUI TGTACA 1 cut(s) 345
BstDEI CTNAG 8 cut(s) 127, 158, 195, 275, 399, 420, 459, 542
BstDSI CCRYGG 1 cut(s) 369
BstF5I GGATG 1 cut(s) 229
BstFNI CGCG 1 cut(s) 475
BstH2I RGCGCY 2 cut(s) 103, 635
BstHHI GCGC 2 cut(s) 102, 634
BstKTI GATC 3 cut(s) 133, 211, 456
BstMBI GATC 3 cut(s) 130, 208, 453
BstMWI GCNNNNNNNGC 2 cut(s) 99, 653
BstNI CCWGG 1 cut(s) 591
BstSCI CCNGG 1 cut(s) 589
BstUI CGCG 1 cut(s) 475
BstV2I GAAGAC 1 cut(s) 690
BstX2I RGATCY 1 cut(s) 453
BstYI RGATCY 1 cut(s) 453
Bsu36I CCTNAGG 2 cut(s) 195, 459
BtgI CCRYGG 1 cut(s) 369
BtgZI GCGATG 2 cut(s) 391, 662
BtsCI GGATG 1 cut(s) 229
BtsIMutI CAGTG 1 cut(s) 417
CfoI GCGC 2 cut(s) 102, 634
Csp6I GTAC 4 cut(s) 45, 346, 466, 606
CviAII CATG 4 cut(s) 310, 370, 392, 565
CviJI RGCY 8 cut(s) 82, 142, 424, 472, 486, 570, 621, 656
CviKI_1 RGCY 8 cut(s) 82, 142, 424, 472, 486, 570, 621, 656
CviQI GTAC 4 cut(s) 45, 346, 466, 606
DdeI CTNAG 8 cut(s) 127, 158, 195, 275, 399, 420, 459, 542
DpnI GATC 3 cut(s) 132, 210, 455
DpnII GATC 3 cut(s) 130, 208, 453
DrdI GACNNNNNNGTC 1 cut(s) 546
DseDI GACNNNNNNGTC 1 cut(s) 546
Eam1104I CTCTTC 1 cut(s) 279
EarI CTCTTC 1 cut(s) 279
Eco130I CCWWGG 1 cut(s) 369
Eco47III AGCGCT 1 cut(s) 633
Eco81I CCTNAGG 2 cut(s) 195, 459
EcoRI GAATTC 1 cut(s) 695
EcoRII CCWGG 1 cut(s) 589
EcoT14I CCWWGG 1 cut(s) 369
ErhI CCWWGG 1 cut(s) 369
FaeI CATG 4 cut(s) 313, 373, 395, 568
FaqI GGGAC 1 cut(s) 175
FatI CATG 4 cut(s) 309, 369, 391, 564
FbaI TGATCA 1 cut(s) 208
Fnu4HI GCNGC 2 cut(s) 473, 654
FokI GGATG 1 cut(s) 236
Fsp4HI GCNGC 2 cut(s) 473, 654
FspBI CTAG 1 cut(s) 48
GlaI GCGC 2 cut(s) 101, 633
GluI GCNGC 2 cut(s) 473, 654
HaeII RGCGCY 2 cut(s) 103, 635
HhaI GCGC 2 cut(s) 102, 634
Hin1II CATG 4 cut(s) 313, 373, 395, 568
Hin6I GCGC 2 cut(s) 100, 632
HinP1I GCGC 2 cut(s) 100, 632
HinfI GANTC 4 cut(s) 188, 295, 360, 598
HphI GGTGA 2 cut(s) 88, 573
Hpy166II GTNNAC 1 cut(s) 154
Hpy188I TCNGA 1 cut(s) 130
Hpy188III TCNNGA 5 cut(s) 62, 512, 672, 700, 715
Hpy8I GTNNAC 1 cut(s) 154
HpyAV CCTTC 1 cut(s) 67
HpyCH4III ACNGT 2 cut(s) 151, 610
HpyCH4V TGCA 4 cut(s) 176, 313, 341, 616
HpyF10VI GCNNNNNNNGC 2 cut(s) 99, 653
HpyF3I CTNAG 8 cut(s) 127, 158, 195, 275, 399, 420, 459, 542
Hsp92II CATG 4 cut(s) 313, 373, 395, 568
HspAI GCGC 2 cut(s) 100, 632
Ksp22I TGATCA 1 cut(s) 208
Kzo9I GATC 3 cut(s) 130, 208, 453
LweI GCATC 1 cut(s) 163
MaeI CTAG 1 cut(s) 48
MaeIII GTNAC 1 cut(s) 476
MalI GATC 3 cut(s) 132, 210, 455
MboI GATC 3 cut(s) 130, 208, 453
MboII GAAGA 2 cut(s) 296, 695
MflI RGATCY 1 cut(s) 453
MluCI AATT 4 cut(s) 68, 429, 667, 695
MlyI GAGTC 1 cut(s) 197
MmeI TCCRAC 1 cut(s) 575
MseI TTAA 2 cut(s) 356, 639
MslI CAYNNNNRTG 1 cut(s) 563
MspR9I CCNGG 1 cut(s) 591
MvaI CCWGG 1 cut(s) 591
MvnI CGCG 1 cut(s) 475
MwoI GCNNNNNNNGC 2 cut(s) 99, 653
NcoI CCATGG 1 cut(s) 369
NdeII GATC 3 cut(s) 130, 208, 453
NlaIII CATG 4 cut(s) 313, 373, 395, 568
NlaIV GGNNCC 2 cut(s) 455, 502
NmuCI GTSAC 1 cut(s) 476
PfeI GAWTC 3 cut(s) 295, 360, 598
PkrI GCNGC 2 cut(s) 474, 655
PleI GAGTC 1 cut(s) 196
PpsI GAGTC 1 cut(s) 196
Psp6I CCWGG 1 cut(s) 589
PspGI CCWGG 1 cut(s) 589
PspN4I GGNNCC 2 cut(s) 455, 502
PsuI RGATCY 1 cut(s) 453
RsaI GTAC 4 cut(s) 46, 347, 467, 607
RsaNI GTAC 4 cut(s) 45, 346, 466, 606
RseI CAYNNNNRTG 1 cut(s) 563
SaqAI TTAA 2 cut(s) 356, 639
SatI GCNGC 2 cut(s) 473, 654
Sau3AI GATC 3 cut(s) 130, 208, 453
SchI GAGTC 1 cut(s) 197
ScrFI CCNGG 1 cut(s) 591
SfaNI GCATC 1 cut(s) 163
SmiMI CAYNNNNRTG 1 cut(s) 563
SmlI CTYRAG 2 cut(s) 481, 713
SmoI CTYRAG 2 cut(s) 481, 713
SpeI ACTAGT 1 cut(s) 47
Sse9I AATT 4 cut(s) 68, 429, 667, 695
SsiI CCGC 3 cut(s) 116, 473, 653
SspI AATATT 1 cut(s) 215
SspMI CTAG 1 cut(s) 48
StyD4I CCNGG 1 cut(s) 589
StyI CCWWGG 1 cut(s) 369
TaaI ACNGT 2 cut(s) 151, 610
TaqI TCGA 1 cut(s) 659
TasI AATT 4 cut(s) 68, 429, 667, 695
TatI WGTACW 1 cut(s) 345
TauI GCSGC 2 cut(s) 475, 656
TfiI GAWTC 3 cut(s) 295, 360, 598
Tru1I TTAA 2 cut(s) 356, 639
Tru9I TTAA 2 cut(s) 356, 639
TscAI CASTG 1 cut(s) 424
TseFI GTSAC 1 cut(s) 476
Tsp45I GTSAC 1 cut(s) 476
TspDTI ATGAA 3 cut(s) 192, 240, 708
TspGWI ACGGA 1 cut(s) 513
TspRI CASTG 1 cut(s) 424
XapI RAATTY 2 cut(s) 667, 695
XspI CTAG 1 cut(s) 48
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.