AT5G39530

Protein of unknown function (DUF1997)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0003399
Physical Location & Seq
Reverse (-)
15826017 .. 15828035
2019 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G39530.2

Sequence Viewer

Length: 720 bp
ATGGGAGAAATGACGGCGAGGTCTTCGGTTTTATCGTCGGGGATGTTCAGAAGTTACCACCGGCCGGTGACGACGGAGGTGAGATCACTATTACGGTCGAGAGTGATGTGCCAAGTGTCATCCGTAAAACCCGCCACGTATTCATCGAGACTCTCCACAGATATTCCTCTCAACGAGTCTCCTCAGGCTTTGTTCGATGAGTATTTAGAGGATAAATCAAGAGTATTCGAAGCAATGTTTCCAGATAAGCCCAGAAGTCATAGGCTTAACGAGGAAGAATGGAGAATTCAGATGTTACCAATAAACTTCCTCTTCCTTACTGTGTGGCCTGTGGTAGATATGAGGTTAAGATGCAAATCCAACGGTCAAGATTATCCGCCAGATGTACCTTTAGATATAACCAAAGTTCTTGAGCTCAACATGATGAGATGGAAGCTTCAAGGGCTTGACCGAGTAATGGAACCAGCTGATTTCAGTTTGGAAGTCAAAGGAGCATTGTACCCTGACCGAAGAGGAAAGCACACAAGGCTCAGAGGTCAACTAGAGATGAACATAAGCTTTGTTCTTCCTCCGGTTCTTGAGTTGGTACCTGAAGATGTTCGAAGAAACTTGGCCAATGCGGTTTTGACCGGTTTAGTGGAGAATATGAAGCATAAGGTTAATGGGAGCTTGCTTTCTGATTATAGCAGGTTCAAGAATGAAAGAAAATTACAAAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1997

Protein Analysis

239

Amino Acids

27.76

Weight (kDa)

9.39

Isoelectric Point (pI)

50.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1997 PF09366 65 - 209 1.8e-37 Protein of unknown function (DUF1997)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 19
Acc36I ACCTGC 1 cut(s) 678
Acc65I GGTACC 1 cut(s) 586
AccB1I GGYRCC 1 cut(s) 586
AciI CCGC 3 cut(s) 132, 377, 620
AcoI YGGCCR 2 cut(s) 62, 612
AcsI RAATTY 1 cut(s) 285
AcuI CTGAAG 1 cut(s) 612
AfaI GTAC 3 cut(s) 387, 500, 588
AfiI CCNNNNNNNGG 2 cut(s) 64, 457
AgeI ACCGGT 1 cut(s) 629
AgsI TTSAA 2 cut(s) 440, 694
AluBI AGCT 5 cut(s) 415, 436, 467, 558, 669
AluI AGCT 5 cut(s) 415, 436, 467, 558, 669
Alw21I GWGCWC 1 cut(s) 417
Alw26I GTCTC 2 cut(s) 142, 183
AoxI GGCC 3 cut(s) 62, 326, 612
ApoI RAATTY 1 cut(s) 285
AsiGI ACCGGT 1 cut(s) 629
Asp700I GAANNNNTTC 1 cut(s) 597
Asp718I GGTACC 1 cut(s) 586
AsuHPI GGTGA 2 cut(s) 79, 91
AsuII TTCGAA 2 cut(s) 228, 601
AxyI CCTNAGG 1 cut(s) 183
BalI TGGCCA 1 cut(s) 614
BanI GGYRCC 1 cut(s) 586
BanII GRGCYC 1 cut(s) 417
BbsI GAAGAC 1 cut(s) 15
Bbv12I GWGCWC 1 cut(s) 417
BccI CCATC 1 cut(s) 423
BceAI ACGGC 1 cut(s) 30
BcoDI GTCTC 2 cut(s) 142, 183
BfaI CTAG 1 cut(s) 542
BfuAI ACCTGC 1 cut(s) 678
BmiI GGNNCC 2 cut(s) 462, 588
BmsI GCATC 1 cut(s) 341
BpiI GAAGAC 1 cut(s) 15
Bpu14I TTCGAA 2 cut(s) 228, 601
BpuEI CTTGAG 2 cut(s) 431, 599
BsaAI YACGTR 1 cut(s) 138
BsaBI GATNNNNATC 1 cut(s) 355
BsaWI WCCGGW 2 cut(s) 571, 629
Bsc4I CCNNNNNNNGG 2 cut(s) 64, 457
Bse118I RCCGGY 3 cut(s) 60, 64, 629
Bse21I CCTNAGG 1 cut(s) 183
Bse3DI GCAATG 1 cut(s) 240
Bse8I GATNNNNATC 1 cut(s) 355
BseGI GGATG 2 cut(s) 48, 119
BseJI GATNNNNATC 1 cut(s) 355
BseLI CCNNNNNNNGG 2 cut(s) 64, 457
BseMI GCAATG 1 cut(s) 240
BseMII CTCAG 2 cut(s) 197, 544
BseRI GAGGAG 1 cut(s) 171
BseX3I CGGCCG 1 cut(s) 62
Bsh1285I CGRYCG 2 cut(s) 65, 98
BshFI GGCC 3 cut(s) 64, 328, 614
BshNI GGYRCC 1 cut(s) 586
BshTI ACCGGT 1 cut(s) 629
BsiEI CGRYCG 2 cut(s) 65, 98
BsiHKAI GWGCWC 1 cut(s) 417
BsiSI CCGG 4 cut(s) 61, 65, 572, 630
BslI CCNNNNNNNGG 2 cut(s) 64, 457
BsmAI GTCTC 2 cut(s) 142, 183
BsnI GGCC 3 cut(s) 64, 328, 614
Bsp119I TTCGAA 2 cut(s) 228, 601
Bsp1286I GDGCHC 1 cut(s) 417
Bsp143I GATC 1 cut(s) 83
BspACI CCGC 3 cut(s) 132, 377, 620
BspANI GGCC 3 cut(s) 64, 328, 614
BspCNI CTCAG 2 cut(s) 196, 543
BspLI GGNNCC 2 cut(s) 462, 588
BspMI ACCTGC 1 cut(s) 678
BspT104I TTCGAA 2 cut(s) 228, 601
BspT107I GGYRCC 1 cut(s) 586
BsrDI GCAATG 1 cut(s) 240
BsrFI RCCGGY 3 cut(s) 60, 64, 629
BssAI RCCGGY 3 cut(s) 60, 64, 629
BssMI GATC 1 cut(s) 83
Bst4CI ACNGT 3 cut(s) 96, 322, 365
Bst6I CTCTTC 2 cut(s) 317, 505
BstBAI YACGTR 1 cut(s) 138
BstBI TTCGAA 2 cut(s) 228, 601
BstC8I GCNNGC 1 cut(s) 671
BstDEI CTNAG 2 cut(s) 183, 530
BstF5I GGATG 2 cut(s) 48, 119
BstKTI GATC 1 cut(s) 86
BstMAI GTCTC 2 cut(s) 142, 183
BstMBI GATC 1 cut(s) 83
BstMCI CGRYCG 2 cut(s) 65, 98
BstMWI GCNNNNNNNGC 2 cut(s) 442, 526
BstV2I GAAGAC 1 cut(s) 15
BstZI CGGCCG 1 cut(s) 62
Bsu36I CCTNAGG 1 cut(s) 183
BsuRI GGCC 3 cut(s) 64, 328, 614
BtsCI GGATG 2 cut(s) 48, 119
BveI ACCTGC 1 cut(s) 678
Cac8I GCNNGC 1 cut(s) 671
Cfr10I RCCGGY 3 cut(s) 60, 64, 629
Csp6I GTAC 3 cut(s) 386, 499, 587
CspAI ACCGGT 1 cut(s) 629
CviAII CATG 1 cut(s) 421
CviQI GTAC 3 cut(s) 386, 499, 587
DdeI CTNAG 2 cut(s) 183, 530
DpnI GATC 1 cut(s) 85
DpnII GATC 1 cut(s) 83
DrdI GACNNNNNNGTC 1 cut(s) 19
DseDI GACNNNNNNGTC 1 cut(s) 19
EaeI YGGCCR 2 cut(s) 62, 612
EagI CGGCCG 1 cut(s) 62
Eam1104I CTCTTC 2 cut(s) 317, 505
EarI CTCTTC 2 cut(s) 317, 505
EciI GGCGGA 1 cut(s) 366
Ecl136II GAGCTC 1 cut(s) 415
EclXI CGGCCG 1 cut(s) 62
Eco24I GRGCYC 1 cut(s) 417
Eco52I CGGCCG 1 cut(s) 62
Eco53kI GAGCTC 1 cut(s) 415
Eco57I CTGAAG 1 cut(s) 612
Eco81I CCTNAGG 1 cut(s) 183
EcoICRI GAGCTC 1 cut(s) 415
EcoRI GAATTC 1 cut(s) 285
EcoT38I GRGCYC 1 cut(s) 417
FaeI CATG 1 cut(s) 424
FaiI YATR 8 cut(s) 261, 341, 398, 422, 554, 647, 654, 684
FatI CATG 1 cut(s) 420
FauI CCCGC 1 cut(s) 139
FokI GGATG 2 cut(s) 55, 106
FriOI GRGCYC 1 cut(s) 417
FspBI CTAG 1 cut(s) 542
HaeIII GGCC 3 cut(s) 64, 328, 614
HapII CCGG 4 cut(s) 61, 65, 572, 630
Hin1II CATG 1 cut(s) 424
HincII GTYRAC 1 cut(s) 539
HindII GTYRAC 1 cut(s) 539
HindIII AAGCTT 2 cut(s) 434, 556
HinfI GANTC 2 cut(s) 150, 176
HpaII CCGG 4 cut(s) 61, 65, 572, 630
HphI GGTGA 2 cut(s) 79, 91
Hpy166II GTNNAC 1 cut(s) 539
Hpy188I TCNGA 4 cut(s) 50, 291, 533, 679
Hpy188III TCNNGA 8 cut(s) 99, 147, 219, 242, 368, 410, 578, 694
Hpy8I GTNNAC 1 cut(s) 539
Hpy99I CGWCG 2 cut(s) 40, 76
HpyCH4III ACNGT 3 cut(s) 96, 322, 365
HpyCH4IV ACGT 1 cut(s) 137
HpyCH4V TGCA 1 cut(s) 354
HpyF10VI GCNNNNNNNGC 2 cut(s) 442, 526
HpyF3I CTNAG 2 cut(s) 183, 530
HpySE526I ACGT 1 cut(s) 137
Hsp92II CATG 1 cut(s) 424
KpnI GGTACC 1 cut(s) 590
Kzo9I GATC 1 cut(s) 83
LmnI GCTCC 2 cut(s) 491, 666
LweI GCATC 1 cut(s) 341
MaeI CTAG 1 cut(s) 542
MaeII ACGT 1 cut(s) 137
MaeIII GTNAC 3 cut(s) 53, 67, 294
MalI GATC 1 cut(s) 85
MboI GATC 1 cut(s) 83
MboII GAAGA 7 cut(s) 15, 287, 304, 522, 557, 605, 615
MhlI GDGCHC 1 cut(s) 417
MlsI TGGCCA 1 cut(s) 614
MluCI AATT 2 cut(s) 285, 707
MluNI TGGCCA 1 cut(s) 614
MlyI GAGTC 2 cut(s) 144, 185
MmeI TCCRAC 1 cut(s) 384
Mox20I TGGCCA 1 cut(s) 614
MroXI GAANNNNTTC 1 cut(s) 597
MscI TGGCCA 1 cut(s) 614
MseI TTAA 3 cut(s) 267, 347, 660
Msp20I TGGCCA 1 cut(s) 614
MspA1I CMGCKG 1 cut(s) 467
MspI CCGG 4 cut(s) 61, 65, 572, 630
MwoI GCNNNNNNNGC 2 cut(s) 442, 526
NdeII GATC 1 cut(s) 83
NlaIII CATG 1 cut(s) 424
NlaIV GGNNCC 2 cut(s) 462, 588
NmuCI GTSAC 1 cut(s) 67
NspV TTCGAA 2 cut(s) 228, 601
PcsI WCGNNNNNNNCGW 2 cut(s) 32, 143
PdmI GAANNNNTTC 1 cut(s) 597
PinAI ACCGGT 1 cut(s) 629
PleI GAGTC 2 cut(s) 144, 184
PpsI GAGTC 2 cut(s) 144, 184
Ppu21I YACGTR 1 cut(s) 138
Psp124BI GAGCTC 1 cut(s) 417
PspN4I GGNNCC 2 cut(s) 462, 588
PvuII CAGCTG 1 cut(s) 467
RsaI GTAC 3 cut(s) 387, 500, 588
RsaNI GTAC 3 cut(s) 386, 499, 587
SacI GAGCTC 1 cut(s) 417
SaqAI TTAA 3 cut(s) 267, 347, 660
Sau3AI GATC 1 cut(s) 83
SchI GAGTC 2 cut(s) 144, 185
SduI GDGCHC 1 cut(s) 417
SfaNI GCATC 1 cut(s) 341
SfuI TTCGAA 2 cut(s) 228, 601
SmlI CTYRAG 2 cut(s) 410, 578
SmoI CTYRAG 2 cut(s) 410, 578
Sse9I AATT 2 cut(s) 285, 707
SsiI CCGC 3 cut(s) 132, 377, 620
SspMI CTAG 1 cut(s) 542
SstI GAGCTC 1 cut(s) 417
TaaI ACNGT 3 cut(s) 96, 322, 365
TaiI ACGT 1 cut(s) 140
TaqI TCGA 5 cut(s) 98, 146, 195, 228, 601
TaqII GACCGA 2 cut(s) 465, 522
TasI AATT 2 cut(s) 285, 707
Tru1I TTAA 3 cut(s) 267, 347, 660
Tru9I TTAA 3 cut(s) 267, 347, 660
TseFI GTSAC 1 cut(s) 67
Tsp45I GTSAC 1 cut(s) 67
TspDTI ATGAA 4 cut(s) 132, 563, 662, 714
TspGWI ACGGA 2 cut(s) 89, 112
XapI RAATTY 1 cut(s) 285
XmnI GAANNNNTTC 1 cut(s) 597
XspI CTAG 1 cut(s) 542
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.