AT5G45590

Ribosomal protein L35

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0003348
Physical Location & Seq
Reverse (-)
18484179 .. 18486340
2162 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G45590.1

Sequence Viewer

Length: 522 bp
ATGCAGAGGTTTTGCACTAAGCTACGTTCCATTTCTCTCCAATCTAATCGGAATCTCTCTTTTTACGGTGCTTCTCCTCACCGTCTCATTCACCATTCTCCTTCTCCGAGTCGTCACTTAACAACAACAACCCTAGGTTTTGCCAATTCCACCAAATGGAGCTTCGTTCCCGCCGCCGGAGTTTTTCCGTCGAATGTTAGCTCCATGGGCACTACCTTTGTTCCTCATCACTTTGTTCAAGTACGAAATATCACTTCTAAGGAAAAAATGGCAAAATGGAAGAAGAAGTGGAGACCTAGGACTCCAATCACATCAAAAGTCAAGAAAGTCAAGATTAAGTTTTATTCGTCATTCAAAGACAGGTTTAAACCACTAAATGATGGTACCATCCGTCGCTGGAAAGAAGGCAAGCGCCACAACGCACATTTGAAGTCAAAGAAATCGAAGCGTAGATTGAGACAACCTGGACTAGTACCACCAGCATATGCGAAAGTCATGAAGAAACTCAATTTCTGCAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ribosomal_L35p

Protein Analysis

173

Amino Acids

20.06

Weight (kDa)

11.76

Isoelectric Point (pI)

64.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L35p PF01632 111 - 169 8e-16 Ribosomal protein L35
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 383
AccB1I GGYRCC 1 cut(s) 383
AccB7I CCANNNNNTGG 1 cut(s) 156
AciI CCGC 2 cut(s) 171, 174
AfaI GTAC 3 cut(s) 243, 385, 474
AfiI CCNNNNNNNGG 2 cut(s) 156, 176
AgsI TTSAA 3 cut(s) 239, 355, 430
AhlI ACTAGT 1 cut(s) 469
AjnI CCWGG 1 cut(s) 463
AjuI GAANNNNNNNTTGG 2 cut(s) 146, 178
AluBI AGCT 3 cut(s) 22, 162, 201
AluI AGCT 3 cut(s) 22, 162, 201
Alw26I GTCTC 3 cut(s) 89, 286, 451
Asp718I GGTACC 1 cut(s) 383
AspA2I CCTAGG 2 cut(s) 133, 296
AspLEI GCGC 1 cut(s) 414
AsuHPI GGTGA 2 cut(s) 71, 83
AvrII CCTAGG 2 cut(s) 133, 296
BaeGI GKGCMC 1 cut(s) 212
BanI GGYRCC 1 cut(s) 383
BccI CCATC 2 cut(s) 374, 395
BciT130I CCWGG 1 cut(s) 465
BcoDI GTCTC 3 cut(s) 89, 286, 451
BcuI ACTAGT 1 cut(s) 469
BfaI CTAG 3 cut(s) 134, 297, 470
BfoI RGCGCY 1 cut(s) 415
BisI GCNGC 1 cut(s) 174
BlnI CCTAGG 2 cut(s) 133, 296
BlsI GCNGC 1 cut(s) 175
Bme1390I CCNGG 1 cut(s) 465
BmiI GGNNCC 1 cut(s) 385
BmrFI CCNGG 1 cut(s) 465
BsaI GGTCTC 1 cut(s) 286
BsaJI CCNNGG 3 cut(s) 133, 204, 296
Bsc4I CCNNNNNNNGG 2 cut(s) 156, 176
BseBI CCWGG 1 cut(s) 465
BseDI CCNNGG 3 cut(s) 133, 204, 296
BseGI GGATG 1 cut(s) 387
BseLI CCNNNNNNNGG 2 cut(s) 156, 176
BseRI GAGGAG 1 cut(s) 66
BseSI GKGCMC 1 cut(s) 212
BshNI GGYRCC 1 cut(s) 383
BsiSI CCGG 1 cut(s) 177
BslI CCNNNNNNNGG 2 cut(s) 156, 176
BsmAI GTCTC 3 cut(s) 89, 286, 451
BsmBI CGTCTC 1 cut(s) 89
Bso31I GGTCTC 1 cut(s) 286
Bsp1286I GDGCHC 1 cut(s) 212
Bsp19I CCATGG 1 cut(s) 204
BspACI CCGC 2 cut(s) 171, 174
BspHI TCATGA 1 cut(s) 495
BspLI GGNNCC 1 cut(s) 385
BspT107I GGYRCC 1 cut(s) 383
BspTNI GGTCTC 1 cut(s) 286
BssECI CCNNGG 3 cut(s) 133, 204, 296
BssT1I CCWWGG 3 cut(s) 133, 204, 296
Bst2UI CCWGG 1 cut(s) 465
Bst4CI ACNGT 2 cut(s) 68, 83
BstC8I GCNNGC 1 cut(s) 410
BstDEI CTNAG 2 cut(s) 18, 258
BstDSI CCRYGG 1 cut(s) 204
BstF5I GGATG 1 cut(s) 387
BstH2I RGCGCY 1 cut(s) 415
BstHHI GCGC 1 cut(s) 414
BstMAI GTCTC 3 cut(s) 89, 286, 451
BstMWI GCNNNNNNNGC 1 cut(s) 207
BstNI CCWGG 1 cut(s) 465
BstSCI CCNGG 1 cut(s) 463
BstSLI GKGCMC 1 cut(s) 212
BtgI CCRYGG 1 cut(s) 204
BtsCI GGATG 1 cut(s) 387
Cac8I GCNNGC 1 cut(s) 410
CciI TCATGA 1 cut(s) 495
CfoI GCGC 1 cut(s) 414
Csp6I GTAC 3 cut(s) 242, 384, 473
CviAII CATG 2 cut(s) 205, 496
CviJI RGCY 3 cut(s) 22, 162, 201
CviKI_1 RGCY 3 cut(s) 22, 162, 201
CviQI GTAC 3 cut(s) 242, 384, 473
DdeI CTNAG 2 cut(s) 18, 258
DraI TTTAAA 1 cut(s) 367
Eco130I CCWWGG 3 cut(s) 133, 204, 296
Eco31I GGTCTC 1 cut(s) 286
EcoRII CCWGG 1 cut(s) 463
EcoT14I CCWWGG 3 cut(s) 133, 204, 296
ErhI CCWWGG 3 cut(s) 133, 204, 296
Esp3I CGTCTC 1 cut(s) 89
FaeI CATG 2 cut(s) 208, 499
FaiI YATR 4 cut(s) 206, 484, 486, 497
FatI CATG 2 cut(s) 204, 495
FauI CCCGC 1 cut(s) 178
FauNDI CATATG 1 cut(s) 484
Fnu4HI GCNGC 1 cut(s) 174
FokI GGATG 1 cut(s) 374
Fsp4HI GCNGC 1 cut(s) 174
FspBI CTAG 3 cut(s) 134, 297, 470
GlaI GCGC 1 cut(s) 413
GluI GCNGC 1 cut(s) 174
HaeII RGCGCY 1 cut(s) 415
HapII CCGG 1 cut(s) 177
HhaI GCGC 1 cut(s) 414
Hin1II CATG 2 cut(s) 208, 499
Hin6I GCGC 1 cut(s) 412
HinP1I GCGC 1 cut(s) 412
HinfI GANTC 3 cut(s) 52, 109, 301
HpaII CCGG 1 cut(s) 177
HphI GGTGA 2 cut(s) 71, 83
Hpy188I TCNGA 2 cut(s) 51, 108
Hpy188III TCNNGA 3 cut(s) 322, 331, 496
Hpy99I CGWCG 2 cut(s) 193, 396
HpyAV CCTTC 2 cut(s) 111, 398
HpyCH4III ACNGT 2 cut(s) 68, 83
HpyCH4IV ACGT 1 cut(s) 25
HpyCH4V TGCA 3 cut(s) 4, 15, 516
HpyF10VI GCNNNNNNNGC 1 cut(s) 207
HpyF3I CTNAG 2 cut(s) 18, 258
HpySE526I ACGT 1 cut(s) 25
Hsp92II CATG 2 cut(s) 208, 499
HspAI GCGC 1 cut(s) 412
KpnI GGTACC 1 cut(s) 387
LmnI GCTCC 2 cut(s) 159, 206
LpnPI CCDG 6 cut(s) 190, 346, 382, 450, 477, 492
MaeI CTAG 3 cut(s) 134, 297, 470
MaeII ACGT 1 cut(s) 25
MaeIII GTNAC 1 cut(s) 113
MboII GAAGA 3 cut(s) 292, 295, 511
MfeI CAATTG 1 cut(s) 517
MhlI GDGCHC 1 cut(s) 212
MluCI AATT 3 cut(s) 145, 508, 517
MlyI GAGTC 2 cut(s) 118, 295
MnlI CCTC 2 cut(s) 87, 234
MseI TTAA 3 cut(s) 119, 336, 366
MspI CCGG 1 cut(s) 177
MspR9I CCNGG 1 cut(s) 465
MssI GTTTAAAC 1 cut(s) 367
MunI CAATTG 1 cut(s) 517
MvaI CCWGG 1 cut(s) 465
MwoI GCNNNNNNNGC 1 cut(s) 207
NcoI CCATGG 1 cut(s) 204
NdeI CATATG 1 cut(s) 484
NlaIII CATG 2 cut(s) 208, 499
NlaIV GGNNCC 1 cut(s) 385
NmuCI GTSAC 1 cut(s) 113
PagI TCATGA 1 cut(s) 495
PfeI GAWTC 1 cut(s) 52
PflMI CCANNNNNTGG 1 cut(s) 156
PkrI GCNGC 1 cut(s) 175
PleI GAGTC 2 cut(s) 117, 295
PmeI GTTTAAAC 1 cut(s) 367
PpsI GAGTC 2 cut(s) 117, 295
Psp6I CCWGG 1 cut(s) 463
PspGI CCWGG 1 cut(s) 463
PspN4I GGNNCC 1 cut(s) 385
RsaI GTAC 3 cut(s) 243, 385, 474
RsaNI GTAC 3 cut(s) 242, 384, 473
SaqAI TTAA 3 cut(s) 119, 336, 366
SatI GCNGC 1 cut(s) 174
SchI GAGTC 2 cut(s) 118, 295
ScrFI CCNGG 1 cut(s) 465
SduI GDGCHC 1 cut(s) 212
SpeI ACTAGT 1 cut(s) 469
Sse9I AATT 3 cut(s) 145, 508, 517
SsiI CCGC 2 cut(s) 171, 174
SspMI CTAG 3 cut(s) 134, 297, 470
StyD4I CCNGG 1 cut(s) 463
StyI CCWWGG 3 cut(s) 133, 204, 296
TaaI ACNGT 2 cut(s) 68, 83
TaiI ACGT 1 cut(s) 28
TaqI TCGA 2 cut(s) 191, 443
TasI AATT 3 cut(s) 145, 508, 517
TauI GCSGC 1 cut(s) 176
TfiI GAWTC 1 cut(s) 52
Tru1I TTAA 3 cut(s) 119, 336, 366
Tru9I TTAA 3 cut(s) 119, 336, 366
TseFI GTSAC 1 cut(s) 113
Tsp45I GTSAC 1 cut(s) 113
TspDTI ATGAA 1 cut(s) 512
TspGWI ACGGA 2 cut(s) 177, 380
Van91I CCANNNNNTGG 1 cut(s) 156
XmaJI CCTAGG 2 cut(s) 133, 296
XspI CTAG 3 cut(s) 134, 297, 470
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.