MD06G1023100.v1.1

Ribosomal protein L35

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0003348
Physical Location & Seq
Reverse (-)
2887489 .. 2888464
976 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1023100.v1.1.491

Sequence Viewer

Length: 447 bp
ATGGCGATGCAGAGATTATTCTCCAAGCTTCGTTCTTTCTCTGTGCACTCAGCCCAAACTCTCCCATCATCTCTCTCTTCTCCTTCATTGCTTCGGTCTTGCCCTCCGCTGCTTCACCAGCCTCTCCCTTTCGCATCAATTGATAACAGATGGATCGTCAGGTCCCTTTTCAGTGCCCATTCACCGACCCAACCTTCACTGGCTGTCACTGCTCTTCGCTCCTCGCCATTCCCTTTCTCGATGGTTCAAGTGCGGCACGTTTCGTCAAGGGAGCGTAAGAAGCGGAGAAAGCCGATGACTCCCGTCACATCCAAGTTAAAGAAAACCAAATTAAAGTCTTACTCGTCTTACAAGTCCAGGTTTAGGACAATGAATGATGGGACTATTCGGCGGTGGAGGGCTGGCAAGCGGCACAATGCCCATTCGAAGGTGTGCTTTGCATTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ribosomal_L35p

Protein Analysis

149

Amino Acids

16.97

Weight (kDa)

12.0

Isoelectric Point (pI)

78.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L35p PF01632 110 - 143 3.1e-07 Ribosomal protein L35
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 5 cut(s) 107, 253, 283, 391, 409
AclWI GGATC 1 cut(s) 161
AfiI CCNNNNNNNGG 2 cut(s) 363, 427
AgsI TTSAA 1 cut(s) 248
AjnI CCWGG 1 cut(s) 356
AluBI AGCT 1 cut(s) 28
AluI AGCT 1 cut(s) 28
Alw21I GWGCWC 1 cut(s) 48
Alw44I GTGCAC 1 cut(s) 44
AlwI GGATC 1 cut(s) 161
ApaLI GTGCAC 1 cut(s) 44
ApeKI GCWGC 1 cut(s) 109
AspS9I GGNCC 1 cut(s) 162
AsuHPI GGTGA 2 cut(s) 107, 174
AsuII TTCGAA 1 cut(s) 425
AvaII GGWCC 1 cut(s) 162
BaeGI GKGCMC 2 cut(s) 48, 178
Bbv12I GWGCWC 1 cut(s) 48
BbvI GCAGC 1 cut(s) 96
BccI CCATC 4 cut(s) 73, 144, 235, 371
BciT130I CCWGG 1 cut(s) 358
BisI GCNGC 3 cut(s) 110, 254, 410
BlsI GCNGC 3 cut(s) 111, 255, 411
Bme1390I CCNGG 1 cut(s) 358
Bme18I GGWCC 1 cut(s) 162
BmgT120I GGNCC 1 cut(s) 162
BmiI GGNNCC 1 cut(s) 164
BmrFI CCNGG 1 cut(s) 358
BmsI GCATC 1 cut(s) 143
BoxI GACNNNNGTC 1 cut(s) 302
Bpu14I TTCGAA 1 cut(s) 425
Bsc4I CCNNNNNNNGG 2 cut(s) 363, 427
Bse1I ACTGG 1 cut(s) 204
Bse3DI GCAATG 1 cut(s) 86
BseBI CCWGG 1 cut(s) 358
BseGI GGATG 1 cut(s) 308
BseLI CCNNNNNNNGG 2 cut(s) 363, 427
BseMI GCAATG 1 cut(s) 86
BseMII CTCAG 1 cut(s) 63
BseNI ACTGG 1 cut(s) 204
BseRI GAGGAG 1 cut(s) 211
BseSI GKGCMC 2 cut(s) 48, 178
BseXI GCAGC 1 cut(s) 96
BsiHKAI GWGCWC 1 cut(s) 48
BslFI GGGAC 2 cut(s) 148, 394
BslI CCNNNNNNNGG 2 cut(s) 363, 427
BsmFI GGGAC 2 cut(s) 148, 394
Bsp119I TTCGAA 1 cut(s) 425
Bsp1286I GDGCHC 2 cut(s) 48, 178
Bsp143I GATC 1 cut(s) 153
BspACI CCGC 5 cut(s) 107, 253, 283, 391, 409
BspCNI CTCAG 1 cut(s) 62
BspLI GGNNCC 1 cut(s) 164
BspPI GGATC 1 cut(s) 161
BspQI GCTCTTC 1 cut(s) 219
BspT104I TTCGAA 1 cut(s) 425
BsrDI GCAATG 1 cut(s) 86
BsrI ACTGG 1 cut(s) 204
BssMI GATC 1 cut(s) 153
Bst2UI CCWGG 1 cut(s) 358
Bst6I CTCTTC 2 cut(s) 82, 219
BstBI TTCGAA 1 cut(s) 425
BstC8I GCNNGC 2 cut(s) 403, 407
BstDEI CTNAG 1 cut(s) 49
BstF5I GGATG 1 cut(s) 308
BstKTI GATC 1 cut(s) 156
BstMBI GATC 1 cut(s) 153
BstMWI GCNNNNNNNGC 4 cut(s) 118, 209, 280, 289
BstNI CCWGG 1 cut(s) 358
BstPAI GACNNNNGTC 1 cut(s) 302
BstSCI CCNGG 1 cut(s) 356
BstSLI GKGCMC 2 cut(s) 48, 178
BstV1I GCAGC 1 cut(s) 96
BtgZI GCGATG 1 cut(s) 20
BtsCI GGATG 1 cut(s) 308
BtsI GCAGTG 1 cut(s) 207
BtsIMutI CAGTG 3 cut(s) 178, 197, 207
Cac8I GCNNGC 2 cut(s) 403, 407
Cfr13I GGNCC 1 cut(s) 162
CviJI RGCY 6 cut(s) 28, 53, 121, 203, 292, 401
CviKI_1 RGCY 6 cut(s) 28, 53, 121, 203, 292, 401
DdeI CTNAG 1 cut(s) 49
DpnI GATC 1 cut(s) 155
DpnII GATC 1 cut(s) 153
Eam1104I CTCTTC 2 cut(s) 82, 219
EarI CTCTTC 2 cut(s) 82, 219
Eco47I GGWCC 1 cut(s) 162
EcoO109I RGGNCCY 1 cut(s) 162
EcoRII CCWGG 1 cut(s) 356
FalI AAGNNNNNCTT 1 cut(s) 419
FaqI GGGAC 2 cut(s) 148, 394
Fnu4HI GCNGC 3 cut(s) 110, 254, 410
FokI GGATG 1 cut(s) 295
Fsp4HI GCNGC 3 cut(s) 110, 254, 410
GluI GCNGC 3 cut(s) 110, 254, 410
HindIII AAGCTT 1 cut(s) 26
HinfI GANTC 1 cut(s) 298
HphI GGTGA 2 cut(s) 107, 174
Hpy166II GTNNAC 1 cut(s) 46
Hpy188III TCNNGA 1 cut(s) 238
Hpy8I GTNNAC 1 cut(s) 46
HpyAV CCTTC 3 cut(s) 93, 204, 421
HpyCH4IV ACGT 1 cut(s) 258
HpyCH4V TGCA 3 cut(s) 10, 46, 440
HpyF10VI GCNNNNNNNGC 4 cut(s) 118, 209, 280, 289
HpyF3I CTNAG 1 cut(s) 49
HpySE526I ACGT 1 cut(s) 258
Kzo9I GATC 1 cut(s) 153
LguI GCTCTTC 1 cut(s) 219
LmnI GCTCC 2 cut(s) 224, 271
LpnPI CCDG 6 cut(s) 131, 145, 185, 343, 370, 387
Lsp1109I GCAGC 1 cut(s) 96
LweI GCATC 1 cut(s) 143
MaeII ACGT 1 cut(s) 258
MaeIII GTNAC 2 cut(s) 205, 304
MalI GATC 1 cut(s) 155
MboI GATC 1 cut(s) 153
MboII GAAGA 2 cut(s) 69, 206
MfeI CAATTG 1 cut(s) 138
MhlI GDGCHC 2 cut(s) 48, 178
MluCI AATT 2 cut(s) 138, 329
MlyI GAGTC 1 cut(s) 292
MnlI CCTC 4 cut(s) 114, 132, 232, 390
MseI TTAA 2 cut(s) 317, 332
MspA1I CMGCKG 1 cut(s) 109
MspR9I CCNGG 1 cut(s) 358
MunI CAATTG 1 cut(s) 138
MvaI CCWGG 1 cut(s) 358
MwoI GCNNNNNNNGC 4 cut(s) 118, 209, 280, 289
NdeII GATC 1 cut(s) 153
NlaIV GGNNCC 1 cut(s) 164
NmuCI GTSAC 2 cut(s) 205, 304
NspV TTCGAA 1 cut(s) 425
PciSI GCTCTTC 1 cut(s) 219
PkrI GCNGC 3 cut(s) 111, 255, 411
PleI GAGTC 1 cut(s) 292
PpsI GAGTC 1 cut(s) 292
PpuMI RGGWCCY 1 cut(s) 162
PshAI GACNNNNGTC 1 cut(s) 302
Psp5II RGGWCCY 1 cut(s) 162
Psp6I CCWGG 1 cut(s) 356
PspGI CCWGG 1 cut(s) 356
PspN4I GGNNCC 1 cut(s) 164
PspPI GGNCC 1 cut(s) 162
PspPPI RGGWCCY 1 cut(s) 162
SapI GCTCTTC 1 cut(s) 219
SaqAI TTAA 2 cut(s) 317, 332
SatI GCNGC 3 cut(s) 110, 254, 410
Sau3AI GATC 1 cut(s) 153
Sau96I GGNCC 1 cut(s) 162
SchI GAGTC 1 cut(s) 292
ScrFI CCNGG 1 cut(s) 358
SduI GDGCHC 2 cut(s) 48, 178
SetI ASST 6 cut(s) 30, 164, 196, 261, 362, 432
SfaNI GCATC 1 cut(s) 143
SfuI TTCGAA 1 cut(s) 425
SinI GGWCC 1 cut(s) 162
Sse9I AATT 2 cut(s) 138, 329
SsiI CCGC 5 cut(s) 107, 253, 283, 391, 409
StyD4I CCNGG 1 cut(s) 356
TaiI ACGT 1 cut(s) 261
TaqI TCGA 2 cut(s) 239, 425
TaqII GACCGA 1 cut(s) 84
TasI AATT 2 cut(s) 138, 329
TauI GCSGC 2 cut(s) 256, 412
Tru1I TTAA 2 cut(s) 317, 332
Tru9I TTAA 2 cut(s) 317, 332
TscAI CASTG 3 cut(s) 178, 204, 214
TseFI GTSAC 2 cut(s) 205, 304
TseI GCWGC 1 cut(s) 109
Tsp45I GTSAC 2 cut(s) 205, 304
TspDTI ATGAA 2 cut(s) 75, 386
TspRI CASTG 3 cut(s) 178, 204, 214
VneI GTGCAC 1 cut(s) 44
VpaK11BI GGWCC 1 cut(s) 162
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.