FvH4_5g23590

Ribosomal protein L35

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0003348
Physical Location & Seq
Forward (+)
14916478 .. 14923467
6990 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g23590.t2

Sequence Viewer

Length: 462 bp
ATGCTGCGATTATGCTCCAAGCTCCGCTCTTTACCCTTGCAATCTCTCTCCTCTTCTTCTTCGTTTCTTCGATCTTCTCCTCGGCTCCACCGCCCTCTCCATTTCGCTTCAAGTAGCGCCAGTTCATGGAGCTCCGTCTTCAATTCACCGACCCAACCGTCACTGCTCGCCGCCACTCTTCGCTCTCAACCATTTCCCATCTCAATGGTTCAAGTGCGGCATGTTTCGTCAAGAGAGCGTAAGAAGAGGAGAAAGCCAATGACACCGGTTACTTCCAAAGTAAAGAAAGTCAAAATCAAGGGTTACTCGTTATTGAATGATGGCACTATTCGACGCTGGAGGGAGGGCAAGCGGCACAATGCACATTTGAAGTCGAAGATATCCAAACGTAGACTTAGACTACCTGCCCTTGTCCATCCTGCTTACGCCACAGTGATGAAGAAGCTTGGTTTTTGCGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ribosomal_L35p

Protein Analysis

154

Amino Acids

17.36

Weight (kDa)

12.0

Isoelectric Point (pI)

79.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L35p PF01632 98 - 156 1.2e-13 Ribosomal protein L35
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 157
Acc36I ACCTGC 1 cut(s) 412
AccB7I CCANNNNNTGG 1 cut(s) 126
AccBSI CCGCTC 1 cut(s) 27
AccI GTMKAC 1 cut(s) 391
AciI CCGC 5 cut(s) 25, 91, 171, 217, 352
AfiI CCNNNNNNNGG 1 cut(s) 126
AgeI ACCGGT 1 cut(s) 265
AgsI TTSAA 5 cut(s) 111, 142, 212, 316, 370
AluBI AGCT 3 cut(s) 22, 132, 445
AluI AGCT 3 cut(s) 22, 132, 445
Alw21I GWGCWC 1 cut(s) 134
ApeKI GCWGC 1 cut(s) 4
AsiGI ACCGGT 1 cut(s) 265
AspLEI GCGC 2 cut(s) 119, 458
AsuHPI GGTGA 1 cut(s) 138
BanII GRGCYC 1 cut(s) 134
BbsI GAAGAC 1 cut(s) 130
Bbv12I GWGCWC 1 cut(s) 134
BccI CCATC 3 cut(s) 206, 314, 423
BfoI RGCGCY 1 cut(s) 120
BfuAI ACCTGC 1 cut(s) 412
BisI GCNGC 4 cut(s) 5, 171, 218, 353
BlsI GCNGC 4 cut(s) 6, 172, 219, 354
BmiI GGNNCC 1 cut(s) 86
BpiI GAAGAC 1 cut(s) 130
BpmI CTGGAG 1 cut(s) 358
BsaJI CCNNGG 1 cut(s) 80
BsaWI WCCGGW 1 cut(s) 265
Bsc4I CCNNNNNNNGG 1 cut(s) 126
Bse118I RCCGGY 1 cut(s) 265
Bse1I ACTGG 1 cut(s) 120
BseDI CCNNGG 1 cut(s) 80
BseGI GGATG 1 cut(s) 415
BseLI CCNNNNNNNGG 1 cut(s) 126
BseNI ACTGG 1 cut(s) 120
BseRI GAGGAG 3 cut(s) 40, 69, 262
BshTI ACCGGT 1 cut(s) 265
BsiHKAI GWGCWC 1 cut(s) 134
BsiSI CCGG 1 cut(s) 266
BslI CCNNNNNNNGG 1 cut(s) 126
Bsp1286I GDGCHC 1 cut(s) 134
Bsp143I GATC 1 cut(s) 71
BspACI CCGC 5 cut(s) 25, 91, 171, 217, 352
BspLI GGNNCC 1 cut(s) 86
BspMI ACCTGC 1 cut(s) 412
BsrBI CCGCTC 1 cut(s) 27
BsrFI RCCGGY 1 cut(s) 265
BsrI ACTGG 1 cut(s) 120
BssAI RCCGGY 1 cut(s) 265
BssECI CCNNGG 1 cut(s) 80
BssMI GATC 1 cut(s) 71
Bst4CI ACNGT 2 cut(s) 159, 433
Bst6I CTCTTC 3 cut(s) 58, 183, 239
BstC8I GCNNGC 2 cut(s) 168, 350
BstDEI CTNAG 1 cut(s) 395
BstF5I GGATG 1 cut(s) 415
BstH2I RGCGCY 1 cut(s) 120
BstHHI GCGC 2 cut(s) 119, 458
BstKTI GATC 1 cut(s) 74
BstMBI GATC 1 cut(s) 71
BstNSI RCATGY 1 cut(s) 224
BstV2I GAAGAC 1 cut(s) 130
BstXI CCANNNNNNTGG 1 cut(s) 205
BtsCI GGATG 1 cut(s) 415
BtsI GCAGTG 1 cut(s) 161
BtsIMutI CAGTG 2 cut(s) 161, 438
BveI ACCTGC 1 cut(s) 412
Cac8I GCNNGC 2 cut(s) 168, 350
CfoI GCGC 2 cut(s) 119, 458
Cfr10I RCCGGY 1 cut(s) 265
CseI GACGC 1 cut(s) 342
CspAI ACCGGT 1 cut(s) 265
CviAII CATG 2 cut(s) 126, 221
CviJI RGCY 5 cut(s) 22, 85, 132, 256, 445
CviKI_1 RGCY 5 cut(s) 22, 85, 132, 256, 445
DdeI CTNAG 1 cut(s) 395
DpnI GATC 1 cut(s) 73
DpnII GATC 1 cut(s) 71
DrdI GACNNNNNNGTC 1 cut(s) 157
DseDI GACNNNNNNGTC 1 cut(s) 157
Eam1104I CTCTTC 3 cut(s) 58, 183, 239
EarI CTCTTC 3 cut(s) 58, 183, 239
Ecl136II GAGCTC 1 cut(s) 132
Eco24I GRGCYC 1 cut(s) 134
Eco32I GATATC 1 cut(s) 381
Eco53kI GAGCTC 1 cut(s) 132
EcoICRI GAGCTC 1 cut(s) 132
EcoRV GATATC 1 cut(s) 381
EcoT38I GRGCYC 1 cut(s) 134
FaeI CATG 2 cut(s) 129, 224
FaiI YATR 3 cut(s) 13, 127, 222
FatI CATG 2 cut(s) 125, 220
FblI GTMKAC 1 cut(s) 391
Fnu4HI GCNGC 4 cut(s) 5, 171, 218, 353
FokI GGATG 1 cut(s) 402
FriOI GRGCYC 1 cut(s) 134
Fsp4HI GCNGC 4 cut(s) 5, 171, 218, 353
GlaI GCGC 2 cut(s) 118, 457
GluI GCNGC 4 cut(s) 5, 171, 218, 353
GsuI CTGGAG 1 cut(s) 358
HaeII RGCGCY 1 cut(s) 120
HapII CCGG 1 cut(s) 266
HgaI GACGC 1 cut(s) 342
HhaI GCGC 2 cut(s) 119, 458
Hin1II CATG 2 cut(s) 129, 224
Hin6I GCGC 2 cut(s) 117, 456
HinP1I GCGC 2 cut(s) 117, 456
HindIII AAGCTT 1 cut(s) 443
HpaII CCGG 1 cut(s) 266
HphI GGTGA 1 cut(s) 138
Hpy166II GTNNAC 1 cut(s) 392
Hpy188III TCNNGA 1 cut(s) 231
Hpy8I GTNNAC 1 cut(s) 392
Hpy99I CGWCG 1 cut(s) 336
HpyCH4III ACNGT 2 cut(s) 159, 433
HpyCH4IV ACGT 1 cut(s) 388
HpyCH4V TGCA 2 cut(s) 40, 362
HpyF3I CTNAG 1 cut(s) 395
HpySE526I ACGT 1 cut(s) 388
Hsp92II CATG 2 cut(s) 129, 224
HspAI GCGC 2 cut(s) 117, 456
Kzo9I GATC 1 cut(s) 71
LmnI GCTCC 5 cut(s) 20, 27, 90, 129, 137
LpnPI CCDG 5 cut(s) 133, 279, 322, 417, 432
MaeII ACGT 1 cut(s) 388
MaeIII GTNAC 3 cut(s) 159, 268, 302
MalI GATC 1 cut(s) 73
MbiI CCGCTC 1 cut(s) 27
MboI GATC 1 cut(s) 71
MhlI GDGCHC 1 cut(s) 134
MluCI AATT 1 cut(s) 142
MnlI CCTC 6 cut(s) 61, 90, 105, 240, 333, 337
MslI CAYNNNNRTG 2 cut(s) 203, 434
MspI CCGG 1 cut(s) 266
NdeII GATC 1 cut(s) 71
NlaIII CATG 2 cut(s) 129, 224
NlaIV GGNNCC 1 cut(s) 86
NmeAIII GCCGAG 1 cut(s) 61
NmuCI GTSAC 1 cut(s) 159
NspI RCATGY 1 cut(s) 224
PflMI CCANNNNNTGG 1 cut(s) 126
PinAI ACCGGT 1 cut(s) 265
PkrI GCNGC 4 cut(s) 6, 172, 219, 354
Psp124BI GAGCTC 1 cut(s) 134
PspN4I GGNNCC 1 cut(s) 86
PsrI GAACNNNNNNTAC 2 cut(s) 106, 138
RseI CAYNNNNRTG 2 cut(s) 203, 434
SacI GAGCTC 1 cut(s) 134
SatI GCNGC 4 cut(s) 5, 171, 218, 353
Sau3AI GATC 1 cut(s) 71
SduI GDGCHC 1 cut(s) 134
SetI ASST 5 cut(s) 24, 134, 391, 406, 447
SmiMI CAYNNNNRTG 2 cut(s) 203, 434
Sse9I AATT 1 cut(s) 142
SsiI CCGC 5 cut(s) 25, 91, 171, 217, 352
SstI GAGCTC 1 cut(s) 134
TaaI ACNGT 2 cut(s) 159, 433
TaiI ACGT 1 cut(s) 391
TaqI TCGA 3 cut(s) 70, 331, 374
TasI AATT 1 cut(s) 142
TauI GCSGC 3 cut(s) 173, 220, 355
TscAI CASTG 2 cut(s) 168, 438
TseFI GTSAC 1 cut(s) 159
TseI GCWGC 1 cut(s) 4
Tsp45I GTSAC 1 cut(s) 159
TspDTI ATGAA 2 cut(s) 114, 452
TspGWI ACGGA 1 cut(s) 124
TspRI CASTG 2 cut(s) 168, 438
Van91I CCANNNNNTGG 1 cut(s) 126
XceI RCATGY 1 cut(s) 224
XmiI GTMKAC 1 cut(s) 391
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.