MD00G1000200.v1.1
ERF Family

Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0003348
Physical Location & Seq
Forward (+)
38005 .. 43167
5163 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1000200.v1.1.491

Sequence Viewer

Length: 522 bp
ATGGCGATGCAGAGATTATTCTCCAAGCTTCGTTCTTTCGCTGTGCGCTCAGCCCAGACTCTCCCATCATCACTCTCCTCTCCTGCATTTCTTCGGTCTTGCCCTCCTCTGCTTCACCACCCTCTCCCTTTCGCTTCAATTCGTAACAAATGGACCGTCAGGTCCCTTTTCATTGCCCATTCACCGATCCAATCATTGCCGCCGGCTCTCACGGCGCTTCATTCCTCGGCATTCCCTTTCTCGATGGTTCAACTGCGGCATGTTTCGTCGAGGGAACGAAAGAAGAGGAGAAAGCCAATGACTCCAGTCACTTCCAAGTTAAAGAAAACCAAAATGAAGTCTTACTCGTCTTACAAGTCCAGGTTTAGGACAATGAATGATGGGACCATTCGGCGGTGGAGGGCGGGCAAGCGGCACAATGCCCATTTGAAGTCAAAGATATCTAAGCGTAGACTCAGACTACCTGCCCTTGTCCATCCTGCTTACGCTACAGTGATGAAGAAGCTCAATTTTTGCGGTTAA

Protein Analysis

174

Amino Acids

19.88

Weight (kDa)

12.0

Isoelectric Point (pI)

79.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L35p PF01632 111 - 169 9.6e-15 Ribosomal protein L35
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 160
Acc36I ACCTGC 1 cut(s) 472
AccI GTMKAC 1 cut(s) 451
AciI CCGC 6 cut(s) 200, 256, 394, 404, 412, 516
AclWI GGATC 1 cut(s) 181
AfiI CCNNNNNNNGG 2 cut(s) 366, 393
AgsI TTSAA 3 cut(s) 138, 251, 430
AjnI CCWGG 1 cut(s) 359
AluBI AGCT 2 cut(s) 28, 505
AluI AGCT 2 cut(s) 28, 505
AlwI GGATC 1 cut(s) 181
ArsI GACNNNNNNTTYG 2 cut(s) 141, 173
AspLEI GCGC 2 cut(s) 48, 217
AspS9I GGNCC 3 cut(s) 153, 162, 384
AsuHPI GGTGA 2 cut(s) 107, 174
AvaII GGWCC 3 cut(s) 153, 162, 384
BccI CCATC 4 cut(s) 73, 238, 374, 483
BceAI ACGGC 1 cut(s) 228
BciT130I CCWGG 1 cut(s) 361
BfmI CTRYAG 1 cut(s) 489
BfoI RGCGCY 1 cut(s) 218
BfuAI ACCTGC 1 cut(s) 472
BisI GCNGC 3 cut(s) 200, 257, 413
BlpI GCTNAGC 1 cut(s) 49
BlsI GCNGC 3 cut(s) 201, 258, 414
Bme1390I CCNGG 1 cut(s) 361
Bme18I GGWCC 3 cut(s) 153, 162, 384
BmgT120I GGNCC 3 cut(s) 153, 162, 384
BmiI GGNNCC 2 cut(s) 164, 385
BmrFI CCNGG 1 cut(s) 361
BoxI GACNNNNGTC 1 cut(s) 305
BpmI CTGGAG 1 cut(s) 288
Bpu1102I GCTNAGC 1 cut(s) 49
BsaJI CCNNGG 1 cut(s) 225
Bsc4I CCNNNNNNNGG 2 cut(s) 366, 393
Bse118I RCCGGY 1 cut(s) 202
Bse1I ACTGG 1 cut(s) 305
Bse3DI GCAATG 2 cut(s) 171, 194
BseBI CCWGG 1 cut(s) 361
BseDI CCNNGG 1 cut(s) 225
BseGI GGATG 1 cut(s) 475
BseLI CCNNNNNNNGG 2 cut(s) 366, 393
BseMI GCAATG 2 cut(s) 171, 194
BseMII CTCAG 2 cut(s) 63, 469
BseNI ACTGG 1 cut(s) 305
BseRI GAGGAG 3 cut(s) 67, 96, 301
BsiSI CCGG 1 cut(s) 203
BslFI GGGAC 2 cut(s) 148, 397
BslI CCNNNNNNNGG 2 cut(s) 366, 393
BsmFI GGGAC 2 cut(s) 148, 397
BsmI GAATGC 1 cut(s) 230
Bsp143I GATC 1 cut(s) 186
Bsp1720I GCTNAGC 1 cut(s) 49
BspACI CCGC 6 cut(s) 200, 256, 394, 404, 412, 516
BspCNI CTCAG 2 cut(s) 62, 468
BspLI GGNNCC 2 cut(s) 164, 385
BspMI ACCTGC 1 cut(s) 472
BspPI GGATC 1 cut(s) 181
BsrDI GCAATG 2 cut(s) 171, 194
BsrFI RCCGGY 1 cut(s) 202
BsrI ACTGG 1 cut(s) 305
BssAI RCCGGY 1 cut(s) 202
BssECI CCNNGG 1 cut(s) 225
BssMI GATC 1 cut(s) 186
Bst2UI CCWGG 1 cut(s) 361
Bst4CI ACNGT 2 cut(s) 157, 493
Bst6I CTCTTC 1 cut(s) 278
BstC8I GCNNGC 3 cut(s) 204, 406, 410
BstDEI CTNAG 3 cut(s) 49, 444, 455
BstF5I GGATG 1 cut(s) 475
BstH2I RGCGCY 1 cut(s) 218
BstHHI GCGC 2 cut(s) 48, 217
BstKTI GATC 1 cut(s) 189
BstMBI GATC 1 cut(s) 186
BstMWI GCNNNNNNNGC 1 cut(s) 212
BstNI CCWGG 1 cut(s) 361
BstNSI RCATGY 1 cut(s) 263
BstPAI GACNNNNGTC 1 cut(s) 305
BstSCI CCNGG 1 cut(s) 359
BstSFI CTRYAG 1 cut(s) 489
BtgZI GCGATG 1 cut(s) 20
BtsCI GGATG 1 cut(s) 475
BtsIMutI CAGTG 1 cut(s) 498
BveI ACCTGC 1 cut(s) 472
Cac8I GCNNGC 3 cut(s) 204, 406, 410
CfoI GCGC 2 cut(s) 48, 217
Cfr10I RCCGGY 1 cut(s) 202
Cfr13I GGNCC 3 cut(s) 153, 162, 384
CviAII CATG 1 cut(s) 260
CviJI RGCY 5 cut(s) 28, 53, 206, 295, 505
CviKI_1 RGCY 5 cut(s) 28, 53, 206, 295, 505
DdeI CTNAG 3 cut(s) 49, 444, 455
DpnI GATC 1 cut(s) 188
DpnII GATC 1 cut(s) 186
DrdI GACNNNNNNGTC 1 cut(s) 160
DseDI GACNNNNNNGTC 1 cut(s) 160
Eam1104I CTCTTC 1 cut(s) 278
EarI CTCTTC 1 cut(s) 278
Eco32I GATATC 1 cut(s) 441
Eco47I GGWCC 3 cut(s) 153, 162, 384
EcoO109I RGGNCCY 1 cut(s) 162
EcoRII CCWGG 1 cut(s) 359
EcoRV GATATC 1 cut(s) 441
FaeI CATG 1 cut(s) 263
FaiI YATR 1 cut(s) 261
FaqI GGGAC 2 cut(s) 148, 397
FatI CATG 1 cut(s) 259
FauI CCCGC 1 cut(s) 397
FblI GTMKAC 1 cut(s) 451
Fnu4HI GCNGC 3 cut(s) 200, 257, 413
FokI GGATG 1 cut(s) 462
Fsp4HI GCNGC 3 cut(s) 200, 257, 413
GlaI GCGC 2 cut(s) 47, 216
GluI GCNGC 3 cut(s) 200, 257, 413
GsuI CTGGAG 1 cut(s) 288
HaeII RGCGCY 1 cut(s) 218
HapII CCGG 1 cut(s) 203
HhaI GCGC 2 cut(s) 48, 217
Hin1II CATG 1 cut(s) 263
Hin6I GCGC 2 cut(s) 46, 215
HinP1I GCGC 2 cut(s) 46, 215
HindIII AAGCTT 1 cut(s) 26
HinfI GANTC 3 cut(s) 58, 301, 453
HpaII CCGG 1 cut(s) 203
HphI GGTGA 2 cut(s) 107, 174
Hpy166II GTNNAC 1 cut(s) 452
Hpy188I TCNGA 1 cut(s) 458
Hpy188III TCNNGA 1 cut(s) 241
Hpy8I GTNNAC 1 cut(s) 452
Hpy99I CGWCG 1 cut(s) 271
HpyCH4III ACNGT 2 cut(s) 157, 493
HpyCH4V TGCA 2 cut(s) 10, 86
HpyF10VI GCNNNNNNNGC 1 cut(s) 212
HpyF3I CTNAG 3 cut(s) 49, 444, 455
Hsp92II CATG 1 cut(s) 263
HspAI GCGC 2 cut(s) 46, 215
KroI GCCGGC 1 cut(s) 202
KroNI GCCGGC 1 cut(s) 204
Kzo9I GATC 1 cut(s) 186
LpnPI CCDG 9 cut(s) 68, 96, 145, 216, 318, 346, 373, 477, 492
MaeIII GTNAC 2 cut(s) 143, 307
MalI GATC 1 cut(s) 188
MboI GATC 1 cut(s) 186
MboII GAAGA 3 cut(s) 83, 295, 511
MluCI AATT 2 cut(s) 138, 508
MlyI GAGTC 3 cut(s) 52, 295, 447
MnlI CCTC 8 cut(s) 88, 114, 117, 132, 235, 264, 279, 393
MroNI GCCGGC 1 cut(s) 202
MseI TTAA 2 cut(s) 320, 520
MspI CCGG 1 cut(s) 203
MspR9I CCNGG 1 cut(s) 361
Mva1269I GAATGC 1 cut(s) 230
MvaI CCWGG 1 cut(s) 361
MwoI GCNNNNNNNGC 1 cut(s) 212
NaeI GCCGGC 1 cut(s) 204
NdeII GATC 1 cut(s) 186
NgoMIV GCCGGC 1 cut(s) 202
NlaIII CATG 1 cut(s) 263
NlaIV GGNNCC 2 cut(s) 164, 385
NmeAIII GCCGAG 1 cut(s) 206
NmuCI GTSAC 1 cut(s) 307
NspI RCATGY 1 cut(s) 263
PctI GAATGC 1 cut(s) 230
PdiI GCCGGC 1 cut(s) 204
PkrI GCNGC 3 cut(s) 201, 258, 414
PleI GAGTC 3 cut(s) 52, 295, 447
PpsI GAGTC 3 cut(s) 52, 295, 447
PpuMI RGGWCCY 1 cut(s) 162
PshAI GACNNNNGTC 1 cut(s) 305
Psp5II RGGWCCY 1 cut(s) 162
Psp6I CCWGG 1 cut(s) 359
PspGI CCWGG 1 cut(s) 359
PspN4I GGNNCC 2 cut(s) 164, 385
PspPI GGNCC 3 cut(s) 153, 162, 384
PspPPI RGGWCCY 1 cut(s) 162
SaqAI TTAA 2 cut(s) 320, 520
SatI GCNGC 3 cut(s) 200, 257, 413
Sau3AI GATC 1 cut(s) 186
Sau96I GGNCC 3 cut(s) 153, 162, 384
SchI GAGTC 3 cut(s) 52, 295, 447
ScrFI CCNGG 1 cut(s) 361
SetI ASST 5 cut(s) 30, 164, 365, 466, 507
SfcI CTRYAG 1 cut(s) 489
SinI GGWCC 3 cut(s) 153, 162, 384
Sse9I AATT 2 cut(s) 138, 508
SsiI CCGC 6 cut(s) 200, 256, 394, 404, 412, 516
StyD4I CCNGG 1 cut(s) 359
TaaI ACNGT 2 cut(s) 157, 493
TaqI TCGA 2 cut(s) 242, 269
TaqII GACCGA 1 cut(s) 84
TasI AATT 2 cut(s) 138, 508
TauI GCSGC 3 cut(s) 202, 259, 415
Tru1I TTAA 2 cut(s) 320, 520
Tru9I TTAA 2 cut(s) 320, 520
TscAI CASTG 1 cut(s) 498
TseFI GTSAC 1 cut(s) 307
Tsp45I GTSAC 1 cut(s) 307
TspDTI ATGAA 5 cut(s) 160, 209, 350, 389, 512
TspRI CASTG 1 cut(s) 498
VpaK11BI GGWCC 3 cut(s) 153, 162, 384
XceI RCATGY 1 cut(s) 263
XmiI GTMKAC 1 cut(s) 451
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.