RchiOBHm_Chr7g0212891

Ribosomal protein L35

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0003348
Physical Location & Seq
Forward (+)
30065007 .. 30072154
7148 bp
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UTR
Exon/CDS
Intron
PRQ19053

Sequence Viewer

Length: 507 bp
ATGATGCAGCGATTATGCTCCAAGCTTCGCTCTTTACCCTTACAATCAGCCCACAAGACTCTACCATCTCTCTCCTCTTCCTCTTCCTGGCTTCGATCTTCTCGCCCTCTTCATTTCGCTTCAAATAGCAGCCACACATGGAGCTCAACGTCCCTCTTCAATGCCCATTCACCAACCCAACCTTCACTGCTCCTCGCCTCTCTTCGCTCTTCAACATTTCCCATCTCAATGGTTCAAGTGCGGCATGTTTCGTCAAGCGAGCGTAAGAAGAGGAGAAAGCCAATGACACCAGTTACTTCCAAGATAAAGAAATACAAAATCAAGGGTTACTCGTCTTACAAGTCCCGGTTTAAGTTGTTGAATGATGGCACTATTCGACGCTGGAGGGAGGGCAAGCGGCACAATGCACATTTGAAGTCGAAGATATCCAAGCGTAGACTGAGACTACCTGCCCTCGTCCATCCTGCTTATGCCAAAGTGATGAAGAAGCTCAATTTTTGTGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ribosomal_L35p

Protein Analysis

168

Amino Acids

19.27

Weight (kDa)

11.88

Isoelectric Point (pI)

70.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L35p PF01632 105 - 164 1.8e-14 Ribosomal protein L35
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 457
AccI GTMKAC 1 cut(s) 436
AciI CCGC 2 cut(s) 241, 397
AfiI CCNNNNNNNGG 1 cut(s) 87
AgsI TTSAA 6 cut(s) 123, 160, 213, 236, 361, 415
AjnI CCWGG 1 cut(s) 86
AjuI GAANNNNNNNTTGG 2 cut(s) 166, 198
AluBI AGCT 3 cut(s) 25, 144, 490
AluI AGCT 3 cut(s) 25, 144, 490
Alw21I GWGCWC 1 cut(s) 146
Alw26I GTCTC 1 cut(s) 436
ApeKI GCWGC 2 cut(s) 7, 129
AsuC2I CCSGG 1 cut(s) 346
AsuHPI GGTGA 1 cut(s) 162
BanII GRGCYC 1 cut(s) 146
Bbv12I GWGCWC 1 cut(s) 146
BbvI GCAGC 2 cut(s) 19, 141
BccI CCATC 4 cut(s) 73, 230, 359, 468
BciT130I CCWGG 1 cut(s) 88
BcnI CCSGG 1 cut(s) 346
BcoDI GTCTC 1 cut(s) 436
BfuAI ACCTGC 1 cut(s) 457
BisI GCNGC 4 cut(s) 8, 130, 242, 398
BlsI GCNGC 4 cut(s) 9, 131, 243, 399
Bme1390I CCNGG 2 cut(s) 88, 346
BmrFI CCNGG 2 cut(s) 88, 346
BpmI CTGGAG 1 cut(s) 403
BpuMI CCSGG 1 cut(s) 346
Bsc4I CCNNNNNNNGG 1 cut(s) 87
Bse1I ACTGG 1 cut(s) 290
BseBI CCWGG 1 cut(s) 88
BseGI GGATG 1 cut(s) 460
BseLI CCNNNNNNNGG 1 cut(s) 87
BseMII CTCAG 1 cut(s) 431
BseNI ACTGG 1 cut(s) 290
BseRI GAGGAG 3 cut(s) 64, 182, 286
BseXI GCAGC 2 cut(s) 19, 141
BsiHKAI GWGCWC 1 cut(s) 146
BsiSI CCGG 1 cut(s) 346
BslFI GGGAC 2 cut(s) 136, 328
BslI CCNNNNNNNGG 1 cut(s) 87
BsmAI GTCTC 1 cut(s) 436
BsmFI GGGAC 2 cut(s) 136, 328
Bsp1286I GDGCHC 1 cut(s) 146
Bsp143I GATC 1 cut(s) 95
BspACI CCGC 2 cut(s) 241, 397
BspCNI CTCAG 1 cut(s) 432
BspMI ACCTGC 1 cut(s) 457
BspQI GCTCTTC 1 cut(s) 214
BsrI ACTGG 1 cut(s) 290
BssMI GATC 1 cut(s) 95
Bst2UI CCWGG 1 cut(s) 88
Bst6I CTCTTC 7 cut(s) 82, 88, 114, 161, 207, 214, 263
BstC8I GCNNGC 2 cut(s) 260, 395
BstDEI CTNAG 1 cut(s) 440
BstF5I GGATG 1 cut(s) 460
BstKTI GATC 1 cut(s) 98
BstMAI GTCTC 1 cut(s) 436
BstMBI GATC 1 cut(s) 95
BstNI CCWGG 1 cut(s) 88
BstNSI RCATGY 1 cut(s) 248
BstSCI CCNGG 2 cut(s) 86, 344
BstV1I GCAGC 2 cut(s) 19, 141
BstXI CCANNNNNNTGG 1 cut(s) 229
BtsCI GGATG 1 cut(s) 460
BtsI GCAGTG 1 cut(s) 185
BtsIMutI CAGTG 1 cut(s) 185
BveI ACCTGC 1 cut(s) 457
Cac8I GCNNGC 2 cut(s) 260, 395
CseI GACGC 1 cut(s) 387
CviAII CATG 2 cut(s) 138, 245
CviJI RGCY 7 cut(s) 25, 50, 91, 132, 144, 280, 490
CviKI_1 RGCY 7 cut(s) 25, 50, 91, 132, 144, 280, 490
DdeI CTNAG 1 cut(s) 440
DpnI GATC 1 cut(s) 97
DpnII GATC 1 cut(s) 95
Eam1104I CTCTTC 7 cut(s) 82, 88, 114, 161, 207, 214, 263
EarI CTCTTC 7 cut(s) 82, 88, 114, 161, 207, 214, 263
Ecl136II GAGCTC 1 cut(s) 144
Eco24I GRGCYC 1 cut(s) 146
Eco32I GATATC 1 cut(s) 426
Eco53kI GAGCTC 1 cut(s) 144
EcoICRI GAGCTC 1 cut(s) 144
EcoRII CCWGG 1 cut(s) 86
EcoRV GATATC 1 cut(s) 426
EcoT38I GRGCYC 1 cut(s) 146
FaeI CATG 2 cut(s) 141, 248
FaiI YATR 4 cut(s) 16, 139, 246, 471
FaqI GGGAC 2 cut(s) 136, 328
FatI CATG 2 cut(s) 137, 244
FblI GTMKAC 1 cut(s) 436
Fnu4HI GCNGC 4 cut(s) 8, 130, 242, 398
FokI GGATG 1 cut(s) 447
FriOI GRGCYC 1 cut(s) 146
Fsp4HI GCNGC 4 cut(s) 8, 130, 242, 398
GluI GCNGC 4 cut(s) 8, 130, 242, 398
GsuI CTGGAG 1 cut(s) 403
HapII CCGG 1 cut(s) 346
HgaI GACGC 1 cut(s) 387
Hin1II CATG 2 cut(s) 141, 248
HindIII AAGCTT 1 cut(s) 23
HinfI GANTC 1 cut(s) 58
HpaII CCGG 1 cut(s) 346
HphI GGTGA 1 cut(s) 162
Hpy166II GTNNAC 1 cut(s) 437
Hpy8I GTNNAC 1 cut(s) 437
Hpy99I CGWCG 1 cut(s) 381
HpyAV CCTTC 1 cut(s) 192
HpyCH4IV ACGT 1 cut(s) 149
HpyCH4V TGCA 2 cut(s) 7, 407
HpyF3I CTNAG 1 cut(s) 440
HpySE526I ACGT 1 cut(s) 149
Hsp92II CATG 2 cut(s) 141, 248
Kzo9I GATC 1 cut(s) 95
LguI GCTCTTC 1 cut(s) 214
LmnI GCTCC 3 cut(s) 23, 141, 195
LpnPI CCDG 7 cut(s) 73, 100, 303, 359, 367, 462, 477
Lsp1109I GCAGC 2 cut(s) 19, 141
MaeII ACGT 1 cut(s) 149
MaeIII GTNAC 2 cut(s) 292, 326
MalI GATC 1 cut(s) 97
MboI GATC 1 cut(s) 95
MhlI GDGCHC 1 cut(s) 146
MluCI AATT 1 cut(s) 493
MlyI GAGTC 1 cut(s) 52
MseI TTAA 1 cut(s) 351
MslI CAYNNNNRTG 1 cut(s) 227
MspI CCGG 1 cut(s) 346
MspR9I CCNGG 2 cut(s) 88, 346
MvaI CCWGG 1 cut(s) 88
NciI CCSGG 1 cut(s) 346
NdeII GATC 1 cut(s) 95
NlaIII CATG 2 cut(s) 141, 248
NspI RCATGY 1 cut(s) 248
PciSI GCTCTTC 1 cut(s) 214
PkrI GCNGC 4 cut(s) 9, 131, 243, 399
PleI GAGTC 1 cut(s) 52
PpsI GAGTC 1 cut(s) 52
Psp124BI GAGCTC 1 cut(s) 146
Psp6I CCWGG 1 cut(s) 86
PspGI CCWGG 1 cut(s) 86
RseI CAYNNNNRTG 1 cut(s) 227
SacI GAGCTC 1 cut(s) 146
SapI GCTCTTC 1 cut(s) 214
SaqAI TTAA 1 cut(s) 351
SatI GCNGC 4 cut(s) 8, 130, 242, 398
Sau3AI GATC 1 cut(s) 95
SchI GAGTC 1 cut(s) 52
ScrFI CCNGG 2 cut(s) 88, 346
SduI GDGCHC 1 cut(s) 146
SetI ASST 6 cut(s) 27, 146, 152, 184, 451, 492
SmiMI CAYNNNNRTG 1 cut(s) 227
Sse9I AATT 1 cut(s) 493
SsiI CCGC 2 cut(s) 241, 397
SstI GAGCTC 1 cut(s) 146
StyD4I CCNGG 2 cut(s) 86, 344
TaiI ACGT 1 cut(s) 152
TaqI TCGA 3 cut(s) 94, 376, 419
TasI AATT 1 cut(s) 493
TauI GCSGC 2 cut(s) 244, 400
Tru1I TTAA 1 cut(s) 351
Tru9I TTAA 1 cut(s) 351
TscAI CASTG 1 cut(s) 192
TseI GCWGC 2 cut(s) 7, 129
TspDTI ATGAA 2 cut(s) 101, 497
TspRI CASTG 1 cut(s) 192
XceI RCATGY 1 cut(s) 248
XmiI GTMKAC 1 cut(s) 436
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.