FvH4_1g02020

F-box LRR-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0000551
Physical Location & Seq
Reverse (-)
1061449 .. 1066957
5509 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g02020.t3

Sequence Viewer

Length: 1752 bp
ATGATGGGGAGTATATGCTCTAGGAAGAGAGAGCAATCGGTGATTGAGGAAGAAGTAGCAACTCGTAGACTAGTGTCCGGGAGGTATTGTAAAAGTAGCAGCTCGAAATGGCTAGGAGCGGCATCTTTTCACTGTCGCAATGTGGAGCATTCTCCAGGAGTAGCAGGCATTTGCCCTTCTCTATTGGAATTGTGCATTTCCAAAATATGCATGGACATTGACAAATATAGTTCGTTCTCAATGCTGCCGAGGGATGTGAGTCAGCAGGTCTTTAACGAATTGGTCTGTTCACAATCTCTCAGTGATGTTTCTCTCGAGGCTTTTAGAGATTGTGCTCTTGAGGATATTGGTTTGGGCGAATACCCTTGTGTGGAAGATAGTTGGATGGAGGCCATCTGTTCACAAGGCTCATCTCTACTATCTATTGATATTTCAGGTTCTGAGGTGACAGACTCTGGGTTGGCTCTTTTAAAAGACTGCTCGAACCTCCAATCATTTACTTATAATTACTGTGACCATGTTTCAGAACATGGACTTAAGCACATCAGCGATCTTTCAAATTTGAAAATCTTGAGTTTTAGAAGGAGCAGTGCAATTACTGCTGATGGGATGCGCACTTTCTCCAGCCTTTATAACTTAGAAAAGTTGGATTTGGAGAGGTGTCCAGAGATTCGTGGTGGTTTTGATCATCTTAAAGGTTTGACGAAACTGAAGTCTCTTAATGTTAGATGTTGTAAATGCATCACAGATTCAGACTTAAAGTCCATCTCAGGGCTTACAAACCTAAATGAATTACAGTTATCCAACTGCAACATTACTGATTCTGGAATCTCTTATTTGAAAGGCTTGCAGAAGCTTAATATGTTGAACTTAGAGGGATGCGATGTTACTTCTGCATGTTTGAAATCTATTTCAGCTCTTGTTGCCCTGGCTTACTTGAATCTCAACAGATGCAATGTATCAGATGAAGGATGTGATAAGTTTTCTGGCCTTACAAATTTGAAGGTTCTGAGCCTGGGATTCAACAGAATCACAGATGCGTGTTTGGTGCATCTAAAAGGTTTGACAAATTTGGAGAGCTTGAACCTGGATTCTTGTAAGATTGGTGACCAGGGGCTTGCGAATTTGTCAGGTCTGGTACTCTTAAAGAATTTAGAGTTGTCTGATACTGAAGTTGGAAGCAATGGGATTCGTCATCTCTCAGGGTTAAAGAATCTGGAGAAATTAAACTTGTCATTCACCCAGGTAAGTGACAGTAGTCTGCAAAAGTTAAATGGGCTGACATCTCTCAAATGGCTTAATCTGGATGCTCGTCAAATTACTGATGCTGGACTTGCAGCTATTACAGGTCTTACAAAATTGACGCATCTGGATCTCTTTGGTGCTCGGATATCAGACTCTGGAGCAAACACATTAAAACACTTCAAGAACCTTCAGTCTCTTGAAATATGTGGTGGAGAATTGACTGATGCTGGTGTGAAGAATCTGAAAGATCTTGTCTGTCTGAGATGGCTAAATTTGTCGCAGAACCGCAGCTTGACAGATAAATCTTTGGAAGTGATTTCTGGTTTAACTGCATTGGTGTCGTTAAACATTTCAAATTCATGCATTACCGATGAGGGATTGCATTATTTAAAGCCTTTGAAGAATCTTCGTTCCTTAACCTTGGAGTCCAGTATGGTGACTGCATCTGAAATTAGGAACCTACAGTCTATCGCCCTCCCTAATTTGGTAAGCTTTCGACCAGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_5 LRR_6 LRR_8

Protein Analysis

584

Amino Acids

63.57

Weight (kDa)

5.95

Isoelectric Point (pI)

40.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7885 PF25372 234 - 294 1.5e-07 Leucine Rich Repeat Domain of unknown function (DUF7885)
LRR_8 PF13855 236 - 295 2.8e-06 Leucine rich repeat
LRR_14 PF23598 277 - 511 1.5e-13 Leucine-rich repeat region
LRR_8 PF13855 310 - 368 1.2e-07 Leucine rich repeat
LRR_4 PF12799 332 - 370 8.1e-06 Leucine Rich repeats (2 copies)
LRR_8 PF13855 477 - 534 3.8e-07 Leucine rich repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000551)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G15740
fragaria_vesca FvH4_1g02020 FvH4_1g02020 FvH4_1g02020 FvH4_1g02020 FvH4_1g02020 FvH4_1g02020 FvH4_1g02020 FvH4_6g29180 FvH4_6g29180 FvH4_6g29180 FvH4_6g29180 FvH4_6g29180 FvH4_6g29180 FvH4_6g29180 FvH4_6g29180 FvH4_7g04581
malus_domestica MD02G1018400.v1.1 MD15G1162400.v1.1 MD17G1217000.v1.1
prunus_persica Prupe.3G092900_v2.0.a1 Prupe.3G092900_v2.0.a1 Prupe.3G092900_v2.0.a1 Prupe.3G092900_v2.0.a1 Prupe.3G092900_v2.0.a1 Prupe.3G092900_v2.0.a1 Prupe.3G092900_v2.0.a1 Prupe.3G092900_v2.0.a1 Prupe.3G092900_v2.0.a1 Prupe.3G092900_v2.0.a1 Prupe.7G252400_v2.0.a1 Prupe.7G252400_v2.0.a1 Prupe.7G252400_v2.0.a1 Prupe.7G252400_v2.0.a1 Prupe.7G252400_v2.0.a1 Prupe.7G252400_v2.0.a1 Prupe.7G252400_v2.0.a1 Prupe.7G252400_v2.0.a1 Prupe.7G252400_v2.0.a1 Prupe.7G252400_v2.0.a1 Prupe.7G252400_v2.0.a1 Prupe.7G252400_v2.0.a1 Prupe.7G252400_v2.0.a1 Prupe.7G252400_v2.0.a1 Prupe.7G252400_v2.0.a1 Prupe.7G252400_v2.0.a1
pyrus_communis pycom15g14600 pycom17g22160
rosa_chinensis RchiOBHm_Chr1g0325881 RchiOBHm_Chr2g0087081 RchiOBHm_Chr2g0134401 RchiOBHm_Chr6g0252571
rosa_laevigata RLG00000015828 RLG00000019390
rosa_multiflora Rmu_co8182664.1_g000001 Rmu_sc0005550.1_g000003 Rmu_sc0013657.1_g000009 Rmu_ssc0000064.1_g000031
rosa_roxburghii Rroxscaffold_2G00110270 Rroxscaffold_3G00253750 Rroxscaffold_7G00171870
rosa_rugosa Rorug01G0472200 Rorug01G0472300 Rorug02G0316800 Rorug05G0039000 Rorug05G0039100 Rorug06G0116400
rosa_samantha Rh1DG076800 Rh2AG025600 Rh2AG369200 Rh2BG025300 Rh2BG375300 Rh2CG025900 Rh2CG352900 Rh2CG433900 Rh2DG025800 Rh2DG392400 Rh3DG227300 Rh7BG234800 Rh7CG498200
rosa_wichuraiana Rw1G005860 Rw2G002030 Rw2G030080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 504, 633
Acc16I TGCGCA 1 cut(s) 614
Acc36I ACCTGC 1 cut(s) 256
AccBSI CCGCTC 1 cut(s) 119
AccI GTMKAC 1 cut(s) 67
AciI CCGC 2 cut(s) 119, 1531
AclWI GGATC 1 cut(s) 1380
AcsI RAATTY 7 cut(s) 559, 997, 1069, 1123, 1150, 1516, 1600
AcuI CTGAAG 3 cut(s) 731, 1191, 1418
AfaI GTAC 1 cut(s) 1140
AfiI CCNNNNNNNGG 3 cut(s) 370, 771, 1729
AflII CTTAAG 1 cut(s) 536
AhdI GACNNNNNGTC 1 cut(s) 760
AhlI ACTAGT 1 cut(s) 70
AjnI CCWGG 6 cut(s) 154, 927, 1014, 1086, 1110, 1242
AluBI AGCT 7 cut(s) 102, 856, 917, 1080, 1340, 1536, 1737
AluI AGCT 7 cut(s) 102, 856, 917, 1080, 1340, 1536, 1737
Alw21I GWGCWC 2 cut(s) 337, 1387
Alw26I GTCTC 2 cut(s) 720, 1443
AlwI GGATC 1 cut(s) 1380
AlwNI CAGNNNCTG 3 cut(s) 440, 455, 1400
Ama87I CYCGRG 1 cut(s) 314
AoxI GGCC 2 cut(s) 390, 988
ApeKI GCWGC 4 cut(s) 99, 244, 1337, 1533
ApoI RAATTY 7 cut(s) 559, 997, 1069, 1123, 1150, 1516, 1600
ArsI GACNNNNNNTTYG 2 cut(s) 698, 730
AspLEI GCGC 1 cut(s) 615
AsuC2I CCSGG 1 cut(s) 79
AsuHPI GGTGA 5 cut(s) 52, 457, 1118, 1231, 1693
AvaI CYCGRG 1 cut(s) 314
BaeI ACNNNNGTAYC 2 cut(s) 1158, 1191
Bbv12I GWGCWC 2 cut(s) 337, 1387
BbvI GCAGC 4 cut(s) 111, 231, 1349, 1545
BccI CCATC 5 cut(s) 379, 401, 599, 773, 1503
BcgI CGANNNNNNTGC 2 cut(s) 1566, 1600
BciT130I CCWGG 6 cut(s) 156, 929, 1016, 1088, 1112, 1244
BclI TGATCA 1 cut(s) 685
BcnI CCSGG 1 cut(s) 79
BcoDI GTCTC 2 cut(s) 720, 1443
BcuI ACTAGT 1 cut(s) 70
BfaI CTAG 3 cut(s) 21, 71, 113
BfmI CTRYAG 1 cut(s) 1706
BfrI CTTAAG 1 cut(s) 536
BfuAI ACCTGC 1 cut(s) 256
BglII AGATCT 1 cut(s) 1492
BisI GCNGC 5 cut(s) 100, 120, 245, 1338, 1534
BlsI GCNGC 5 cut(s) 101, 121, 246, 1339, 1535
Bme1390I CCNGG 7 cut(s) 79, 156, 929, 1016, 1088, 1112, 1244
BmeRI GACNNNNNGTC 1 cut(s) 760
BmeT110I CYCGRG 1 cut(s) 314
BmiI GGNNCC 1 cut(s) 1703
BmrFI CCNGG 7 cut(s) 79, 156, 929, 1016, 1088, 1112, 1244
BoxI GACNNNNGTC 2 cut(s) 73, 1257
BpmI CTGGAG 4 cut(s) 138, 607, 1238, 1422
BpuEI CTTGAG 2 cut(s) 359, 592
BpuMI CCSGG 1 cut(s) 79
BsaJI CCNNGG 6 cut(s) 248, 927, 1015, 1111, 1242, 1665
BsaXI ACNNNNNCTCC 2 cut(s) 73, 103
Bsc4I CCNNNNNNNGG 3 cut(s) 370, 771, 1729
Bse1I ACTGG 1 cut(s) 1674
Bse3DI GCAATG 3 cut(s) 145, 961, 1189
BseBI CCWGG 6 cut(s) 156, 929, 1016, 1088, 1112, 1244
BseDI CCNNGG 6 cut(s) 248, 927, 1015, 1111, 1242, 1665
BseGI GGATG 6 cut(s) 259, 390, 615, 884, 977, 1312
BseLI CCNNNNNNNGG 3 cut(s) 370, 771, 1729
BseMI GCAATG 3 cut(s) 145, 961, 1189
BseMII CTCAG 6 cut(s) 313, 432, 783, 1001, 1215, 1496
BseNI ACTGG 1 cut(s) 1674
BseXI GCAGC 4 cut(s) 111, 231, 1349, 1545
BshFI GGCC 2 cut(s) 392, 990
BsiHKAI GWGCWC 2 cut(s) 337, 1387
BsiHKCI CYCGRG 1 cut(s) 314
BsiSI CCGG 1 cut(s) 78
BslI CCNNNNNNNGG 3 cut(s) 370, 771, 1729
BsmAI GTCTC 2 cut(s) 720, 1443
BsmI GAATGC 1 cut(s) 148
BsnI GGCC 2 cut(s) 392, 990
BsoBI CYCGRG 1 cut(s) 314
Bsp1286I GDGCHC 2 cut(s) 337, 1387
Bsp143I GATC 4 cut(s) 550, 685, 1372, 1492
BspACI CCGC 2 cut(s) 119, 1531
BspANI GGCC 2 cut(s) 392, 990
BspCNI CTCAG 6 cut(s) 312, 433, 782, 1002, 1214, 1497
BspLI GGNNCC 1 cut(s) 1703
BspMI ACCTGC 1 cut(s) 256
BspPI GGATC 1 cut(s) 1380
BspTI CTTAAG 1 cut(s) 536
BsrBI CCGCTC 1 cut(s) 119
BsrDI GCAATG 3 cut(s) 145, 961, 1189
BsrI ACTGG 1 cut(s) 1674
BssECI CCNNGG 6 cut(s) 248, 927, 1015, 1111, 1242, 1665
BssMI GATC 4 cut(s) 550, 685, 1372, 1492
BssT1I CCWWGG 1 cut(s) 1665
Bst2UI CCWGG 6 cut(s) 156, 929, 1016, 1088, 1112, 1244
Bst4CI ACNGT 5 cut(s) 134, 512, 798, 1256, 1710
Bst6I CTCTTC 1 cut(s) 20
BstAFI CTTAAG 1 cut(s) 536
BstAPI GCANNNNNTGC 1 cut(s) 599
BstC8I GCNNGC 3 cut(s) 166, 848, 1119
BstDEI CTNAG 8 cut(s) 299, 441, 637, 769, 871, 1010, 1201, 1505
BstEII GGTNACC 1 cut(s) 1106
BstF5I GGATG 6 cut(s) 259, 390, 615, 884, 977, 1312
BstHHI GCGC 1 cut(s) 615
BstKTI GATC 4 cut(s) 553, 688, 1375, 1495
BstMAI GTCTC 2 cut(s) 720, 1443
BstMBI GATC 4 cut(s) 550, 685, 1372, 1492
BstMWI GCNNNNNNNGC 3 cut(s) 599, 923, 1334
BstNI CCWGG 6 cut(s) 156, 929, 1016, 1088, 1112, 1244
BstNSI RCATGY 1 cut(s) 900
BstPAI GACNNNNGTC 2 cut(s) 73, 1257
BstPI GGTNACC 1 cut(s) 1106
BstSCI CCNGG 7 cut(s) 77, 154, 927, 1014, 1086, 1110, 1242
BstSFI CTRYAG 1 cut(s) 1706
BstV1I GCAGC 4 cut(s) 111, 231, 1349, 1545
BstX2I RGATCY 2 cut(s) 1372, 1492
BstYI RGATCY 2 cut(s) 1372, 1492
BsuRI GGCC 2 cut(s) 392, 990
BtgZI GCGATG 1 cut(s) 897
BtsCI GGATG 6 cut(s) 259, 390, 615, 884, 977, 1312
BtsI GCAGTG 1 cut(s) 595
BtsIMutI CAGTG 3 cut(s) 130, 307, 595
BveI ACCTGC 1 cut(s) 256
Cac8I GCNNGC 3 cut(s) 166, 848, 1119
CaiI CAGNNNCTG 3 cut(s) 440, 455, 1400
CfoI GCGC 1 cut(s) 615
CseI GACGC 1 cut(s) 1372
Csp6I GTAC 1 cut(s) 1139
CviAII CATG 5 cut(s) 211, 518, 530, 897, 1605
CviQI GTAC 1 cut(s) 1139
DdeI CTNAG 8 cut(s) 299, 441, 637, 769, 871, 1010, 1201, 1505
DpnI GATC 4 cut(s) 552, 687, 1374, 1494
DpnII GATC 4 cut(s) 550, 685, 1372, 1492
DraI TTTAAA 2 cut(s) 471, 1635
DriI GACNNNNNGTC 1 cut(s) 760
Eam1104I CTCTTC 1 cut(s) 20
Eam1105I GACNNNNNGTC 1 cut(s) 760
EarI CTCTTC 1 cut(s) 20
Eco130I CCWWGG 1 cut(s) 1665
Eco32I GATATC 1 cut(s) 1392
Eco57I CTGAAG 3 cut(s) 731, 1191, 1418
Eco88I CYCGRG 1 cut(s) 314
Eco91I GGTNACC 1 cut(s) 1106
EcoO65I GGTNACC 1 cut(s) 1106
EcoRII CCWGG 6 cut(s) 154, 927, 1014, 1086, 1110, 1242
EcoRV GATATC 1 cut(s) 1392
EcoT14I CCWWGG 1 cut(s) 1665
EcoT22I ATGCAT 3 cut(s) 212, 743, 1610
ErhI CCWWGG 1 cut(s) 1665
FaeI CATG 5 cut(s) 214, 521, 533, 900, 1608
FatI CATG 5 cut(s) 210, 517, 529, 896, 1604
FbaI TGATCA 1 cut(s) 685
FblI GTMKAC 1 cut(s) 67
Fnu4HI GCNGC 5 cut(s) 100, 120, 245, 1338, 1534
FokI GGATG 6 cut(s) 266, 397, 622, 891, 984, 1319
Fsp4HI GCNGC 5 cut(s) 100, 120, 245, 1338, 1534
FspAI RTGCGCAY 1 cut(s) 614
FspBI CTAG 3 cut(s) 21, 71, 113
FspI TGCGCA 1 cut(s) 614
GlaI GCGC 1 cut(s) 614
GluI GCNGC 5 cut(s) 100, 120, 245, 1338, 1534
GsuI CTGGAG 4 cut(s) 138, 607, 1238, 1422
HaeIII GGCC 2 cut(s) 392, 990
HapII CCGG 1 cut(s) 78
HgaI GACGC 1 cut(s) 1372
HhaI GCGC 1 cut(s) 615
Hin1II CATG 5 cut(s) 214, 521, 533, 900, 1608
Hin6I GCGC 1 cut(s) 613
HinP1I GCGC 1 cut(s) 613
HindIII AAGCTT 2 cut(s) 854, 1735
HpaII CCGG 1 cut(s) 78
HphI GGTGA 5 cut(s) 52, 457, 1118, 1231, 1693
Hpy166II GTNNAC 3 cut(s) 68, 290, 401
Hpy8I GTNNAC 3 cut(s) 68, 290, 401
HpyAV CCTTC 5 cut(s) 186, 576, 962, 997, 1442
HpyCH4III ACNGT 5 cut(s) 134, 512, 798, 1256, 1710
HpyF10VI GCNNNNNNNGC 3 cut(s) 599, 923, 1334
HpyF3I CTNAG 8 cut(s) 299, 441, 637, 769, 871, 1010, 1201, 1505
Hsp92II CATG 5 cut(s) 214, 521, 533, 900, 1608
HspAI GCGC 1 cut(s) 613
Ksp22I TGATCA 1 cut(s) 685
Kzo9I GATC 4 cut(s) 550, 685, 1372, 1492
LmnI GCTCC 4 cut(s) 116, 145, 585, 1403
Lsp1109I GCAGC 4 cut(s) 111, 231, 1349, 1545
MaeI CTAG 3 cut(s) 21, 71, 113
MaeIII GTNAC 6 cut(s) 445, 512, 886, 1106, 1250, 1681
MalI GATC 4 cut(s) 552, 687, 1374, 1494
MbiI CCGCTC 1 cut(s) 119
MboI GATC 4 cut(s) 550, 685, 1372, 1492
MboII GAAGA 6 cut(s) 37, 62, 386, 1492, 1643, 1657
MflI RGATCY 2 cut(s) 1372, 1492
MhlI GDGCHC 2 cut(s) 337, 1387
MlyI GAGTC 4 cut(s) 268, 446, 1391, 1679
MmeI TCCRAC 4 cut(s) 362, 627, 828, 1156
Mph1103I ATGCAT 3 cut(s) 212, 743, 1610
MspCI CTTAAG 1 cut(s) 536
MspI CCGG 1 cut(s) 78
MspR9I CCNGG 7 cut(s) 79, 156, 929, 1016, 1088, 1112, 1244
Mva1269I GAATGC 1 cut(s) 148
MvaI CCWGG 6 cut(s) 156, 929, 1016, 1088, 1112, 1244
MwoI GCNNNNNNNGC 3 cut(s) 599, 923, 1334
NciI CCSGG 1 cut(s) 79
NdeII GATC 4 cut(s) 550, 685, 1372, 1492
NlaIII CATG 5 cut(s) 214, 521, 533, 900, 1608
NlaIV GGNNCC 1 cut(s) 1703
NmeAIII GCCGAG 1 cut(s) 273
NmuCI GTSAC 5 cut(s) 445, 512, 1106, 1250, 1681
NsbI TGCGCA 1 cut(s) 614
NsiI ATGCAT 3 cut(s) 212, 743, 1610
NspI RCATGY 1 cut(s) 900
PaeR7I CTCGAG 1 cut(s) 314
PctI GAATGC 1 cut(s) 148
PfoI TCCNGGA 2 cut(s) 77, 154
PkrI GCNGC 5 cut(s) 101, 121, 246, 1339, 1535
PleI GAGTC 4 cut(s) 267, 446, 1391, 1678
PpsI GAGTC 4 cut(s) 267, 446, 1391, 1678
PshAI GACNNNNGTC 2 cut(s) 73, 1257
PsiI TTATAA 2 cut(s) 504, 633
Psp6I CCWGG 6 cut(s) 154, 927, 1014, 1086, 1110, 1242
PspEI GGTNACC 1 cut(s) 1106
PspGI CCWGG 6 cut(s) 154, 927, 1014, 1086, 1110, 1242
PspN4I GGNNCC 1 cut(s) 1703
PstNI CAGNNNCTG 3 cut(s) 440, 455, 1400
PsuI RGATCY 2 cut(s) 1372, 1492
RsaI GTAC 1 cut(s) 1140
RsaNI GTAC 1 cut(s) 1139
SatI GCNGC 5 cut(s) 100, 120, 245, 1338, 1534
Sau3AI GATC 4 cut(s) 550, 685, 1372, 1492
SchI GAGTC 4 cut(s) 268, 446, 1391, 1679
ScrFI CCNGG 7 cut(s) 79, 156, 929, 1016, 1088, 1112, 1244
SduI GDGCHC 2 cut(s) 337, 1387
SfcI CTRYAG 1 cut(s) 1706
Sfr274I CTCGAG 1 cut(s) 314
SlaI CTCGAG 1 cut(s) 314
SmlI CTYRAG 4 cut(s) 314, 338, 536, 571
SmoI CTYRAG 4 cut(s) 314, 338, 536, 571
SpeI ACTAGT 1 cut(s) 70
SsiI CCGC 2 cut(s) 119, 1531
SspMI CTAG 3 cut(s) 21, 71, 113
StyD4I CCNGG 7 cut(s) 77, 154, 927, 1014, 1086, 1110, 1242
StyI CCWWGG 1 cut(s) 1665
TaaI ACNGT 5 cut(s) 134, 512, 798, 1256, 1710
TaqI TCGA 4 cut(s) 104, 315, 482, 1741
TauI GCSGC 1 cut(s) 122
TscAI CASTG 3 cut(s) 137, 307, 595
TseFI GTSAC 5 cut(s) 445, 512, 1106, 1250, 1681
TseI GCWGC 4 cut(s) 99, 244, 1337, 1533
Tsp45I GTSAC 5 cut(s) 445, 512, 1106, 1250, 1681
TspDTI ATGAA 3 cut(s) 804, 981, 1593
TspRI CASTG 3 cut(s) 137, 307, 595
Vha464I CTTAAG 1 cut(s) 536
XapI RAATTY 7 cut(s) 559, 997, 1069, 1123, 1150, 1516, 1600
XceI RCATGY 1 cut(s) 900
XcmI CCANNNNNNNNNTGG 1 cut(s) 208
XhoI CTCGAG 1 cut(s) 314
XmiI GTMKAC 1 cut(s) 67
XspI CTAG 3 cut(s) 21, 71, 113
Zsp2I ATGCAT 3 cut(s) 212, 743, 1610
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.