Rw2G002030

F-box LRR-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0000551
Physical Location & Seq
Reverse (-)
1704110 .. 1709183
5074 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G002030.1

Sequence Viewer

Length: 1698 bp
ATGATGGGGGGTATTTGCTCGAGAAAGAGAGACCAACCGGTCATTGAAGAAGGCGGTGTTAGTAGGGTAGTGTCTGGAAGATATGGTAAAAGTAGCAGTTCAAAATGGCTAGGAACTTCGTCTTTTCGTTCCACTGTAGAACAGTCTCCAGGAGTAGCAGGCATTTGCCCATCTCTCTTGGAATTATGCATTTCCAAATATGCCGGTCAGCAAATCTTTAATGAATTGGTCTCTTCCCAATCTCTCACTCATGATTCTCTCCAGGCTTTTAGAGATTGTGCTCTTGAGGATGTTTGTTTGGGTGAATACCCTTCTGTGAATGATAATTGGATGGATGCCATCTGTTCACAAGGTTCATCTCTACTATCTGTTGATCTCTCTGGTTCCGAGGTGACAGATGCTGGATTGGATCTTCTAAAAGACTGCTCGAACCTCCAATCATTAACTTATAATTACTGTGACCATGTTTCAGAACATGGACTTAAGCACATCAGTGTTTTAAAAGGAGCAGTGCAAATTAGTGCTGAAGGGATGCACGCTTTCTCCAGCCTTTTTAACTTAGAAAAGTTGGACTTGGAGAGGTGTCCTGAGATTCATGGTGGCTTTGTTCATCTTAAAGGTTTGACGAAACTAAAGTCTCTTAATGTTAGATGTTGTAAATGCGTCACAGATTCAGATTTAAAGGCCATCTCAGGGCTTACAAACCTCAATGAATTACAGATATCTAACTGCAACATTACTGATTCTGGAATCTCTTATTTGAAAGGCTTGCAGAAGCTTAATATGTTGAACTTAGAGGGATGCAATGTTACTTCTGCATGTTTGGAATCTATTTCAGCTCTTGTTGCCCTGGCTTACTTAAATCTCAACAGATGCAGTTTATCTGATGAAGGATGTGATAAGTTTTCTGGCCTTACAAACTTGAAGGTTTTGAGCTTGGGATTCAACAGTATCACAGATGCATGTTTGTTGCATCTAAAAGGTTTGACAAATTTGGAGAGCTTGAACCTGGATTCTTGTAAGATAGGTGACGAGGGGCTTGCAAGCTTGGCAGGGTCTGTACTCTTGAAGAATTTGGAGTTATCTGATACTGAAGTTGGAAGCAATGGGATTCGTCATCTCTCAGGGTTAAAGAATCTGGAGAACTTAAACTTGTCGTTCACCCTGGTAACTGACGGTAGTCTGAAAAAGTTATCTGGACTGACATCTCTCAAATCACTTAATCTGGATGCTCGCCAAATTACTGATGCTGGACTTGCAGCTATTACAGGTCTTACAAAATTGACGCATCTGGATCTCTTTGGCGCTCGGATTTCAGACTCTGGAGCAAGCTCTTTAAAACACTTCAAGAACCTTCAATCTCTTGAAATATGTGGTGGAGGATTGACTGATGCTGGTGTGAAGAATCTCAAAGATCTTGTCTGTCTGACATGGCTAAATTTGTCGCAGAACTGCAACTTGACTGATAAATCCTTGGAAGTGATTTCTGGTTTGACTGCATTGGTGTCATTAAACATTTCAAATTCACGCATTACCAATGAGGGATTGCAGTATTTAAAGCCTTTGAAGAATCTACGTTCCTTAACCTTGGAGTCCAGTAAGGTGACTGCATCTGAAATCAGGAAGCTACAGTTTGCTGACCTCCCTAATCTTGTAAGCTTTCGACCAGAATATATTGAAGGCTCGAAGCTTTGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_5 LRR_6 LRR_8

Protein Analysis

565

Amino Acids

60.62

Weight (kDa)

5.83

Isoelectric Point (pI)

36.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 174 - 417 8.6e-11 Leucine-rich repeat region
DUF7885 PF25372 206 - 269 2.5e-06 Leucine Rich Repeat Domain of unknown function (DUF7885)
LRR_8 PF13855 284 - 342 1.4e-06 Leucine rich repeat
DUF7885 PF25372 385 - 449 6e-07 Leucine Rich Repeat Domain of unknown function (DUF7885)
LRR_14 PF23598 398 - 545 1.9e-07 Leucine-rich repeat region
LRR_8 PF13855 451 - 511 1e-07 Leucine rich repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000551)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G15740
fragaria_vesca FvH4_1g02020 FvH4_1g02020 FvH4_1g02020 FvH4_1g02020 FvH4_1g02020 FvH4_1g02020 FvH4_1g02020 FvH4_6g29180 FvH4_6g29180 FvH4_6g29180 FvH4_6g29180 FvH4_6g29180 FvH4_6g29180 FvH4_6g29180 FvH4_6g29180 FvH4_7g04581
malus_domestica MD02G1018400.v1.1 MD15G1162400.v1.1 MD17G1217000.v1.1
prunus_persica Prupe.3G092900_v2.0.a1 Prupe.3G092900_v2.0.a1 Prupe.3G092900_v2.0.a1 Prupe.3G092900_v2.0.a1 Prupe.3G092900_v2.0.a1 Prupe.3G092900_v2.0.a1 Prupe.3G092900_v2.0.a1 Prupe.3G092900_v2.0.a1 Prupe.3G092900_v2.0.a1 Prupe.3G092900_v2.0.a1 Prupe.7G252400_v2.0.a1 Prupe.7G252400_v2.0.a1 Prupe.7G252400_v2.0.a1 Prupe.7G252400_v2.0.a1 Prupe.7G252400_v2.0.a1 Prupe.7G252400_v2.0.a1 Prupe.7G252400_v2.0.a1 Prupe.7G252400_v2.0.a1 Prupe.7G252400_v2.0.a1 Prupe.7G252400_v2.0.a1 Prupe.7G252400_v2.0.a1 Prupe.7G252400_v2.0.a1 Prupe.7G252400_v2.0.a1 Prupe.7G252400_v2.0.a1 Prupe.7G252400_v2.0.a1 Prupe.7G252400_v2.0.a1
pyrus_communis pycom15g14600 pycom17g22160
rosa_chinensis RchiOBHm_Chr1g0325881 RchiOBHm_Chr2g0087081 RchiOBHm_Chr2g0134401 RchiOBHm_Chr6g0252571
rosa_laevigata RLG00000015828 RLG00000019390
rosa_multiflora Rmu_co8182664.1_g000001 Rmu_sc0005550.1_g000003 Rmu_sc0013657.1_g000009 Rmu_ssc0000064.1_g000031
rosa_roxburghii Rroxscaffold_2G00110270 Rroxscaffold_3G00253750 Rroxscaffold_7G00171870
rosa_rugosa Rorug01G0472200 Rorug01G0472300 Rorug02G0316800 Rorug05G0039000 Rorug05G0039100 Rorug06G0116400
rosa_samantha Rh1DG076800 Rh2AG025600 Rh2AG369200 Rh2BG025300 Rh2BG375300 Rh2CG025900 Rh2CG352900 Rh2CG433900 Rh2DG025800 Rh2DG392400 Rh3DG227300 Rh7BG234800 Rh7CG498200
rosa_wichuraiana Rw1G005860 Rw2G002030 Rw2G030080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 450
AasI GACNNNNNNGTC 1 cut(s) 38
AciI CCGC 1 cut(s) 54
AclWI GGATC 2 cut(s) 417, 1302
AcsI RAATTY 4 cut(s) 991, 1072, 1438, 1522
AcuI CTGAAG 2 cut(s) 546, 1113
AfaI GTAC 1 cut(s) 1062
AfiI CCNNNNNNNGG 1 cut(s) 693
AflII CTTAAG 1 cut(s) 482
AgeI ACCGGT 1 cut(s) 37
AjnI CCWGG 5 cut(s) 148, 261, 849, 1008, 1164
AleI CACNNNNGTG 1 cut(s) 492
Alw21I GWGCWC 1 cut(s) 283
Alw26I GTCTC 4 cut(s) 24, 150, 235, 642
AlwI GGATC 2 cut(s) 417, 1302
AlwNI CAGNNNCTG 3 cut(s) 401, 1058, 1322
Ama87I CYCGRG 1 cut(s) 19
AoxI GGCC 2 cut(s) 684, 910
ApeKI GCWGC 1 cut(s) 1259
ApoI RAATTY 4 cut(s) 991, 1072, 1438, 1522
ArsI GACNNNNNNTTYG 4 cut(s) 620, 652, 1404, 1436
AsiGI ACCGGT 1 cut(s) 37
AspLEI GCGC 1 cut(s) 1307
AsuHPI GGTGA 5 cut(s) 314, 403, 1040, 1153, 1615
AvaI CYCGRG 1 cut(s) 19
BaeI ACNNNNGTAYC 2 cut(s) 1080, 1113
Bbv12I GWGCWC 1 cut(s) 283
BbvI GCAGC 1 cut(s) 1271
BccI CCATC 4 cut(s) 178, 325, 347, 695
BcgI CGANNNNNNTGC 2 cut(s) 1022, 1056
BciT130I CCWGG 5 cut(s) 150, 263, 851, 1010, 1166
BcoDI GTCTC 4 cut(s) 24, 150, 235, 642
BfaI CTAG 1 cut(s) 110
BfmI CTRYAG 2 cut(s) 135, 1628
BfoI RGCGCY 1 cut(s) 1308
BfrI CTTAAG 1 cut(s) 482
BglII AGATCT 1 cut(s) 1414
BisI GCNGC 1 cut(s) 1260
BlsI GCNGC 1 cut(s) 1261
Bme1390I CCNGG 5 cut(s) 150, 263, 851, 1010, 1166
BmeT110I CYCGRG 1 cut(s) 19
BmiI GGNNCC 1 cut(s) 385
BmrFI CCNGG 5 cut(s) 150, 263, 851, 1010, 1166
BoxI GACNNNNGTC 1 cut(s) 1179
BpmI CTGGAG 5 cut(s) 132, 245, 529, 1160, 1344
BpuEI CTTGAG 1 cut(s) 305
BsaI GGTCTC 2 cut(s) 24, 235
BsaJI CCNNGG 5 cut(s) 387, 849, 1164, 1473, 1587
BsaWI WCCGGW 1 cut(s) 37
BsaXI ACNNNNNCTCC 2 cut(s) 527, 557
Bsc4I CCNNNNNNNGG 1 cut(s) 693
Bse118I RCCGGY 2 cut(s) 37, 203
Bse1I ACTGG 1 cut(s) 1596
Bse3DI GCAATG 2 cut(s) 811, 1111
BseBI CCWGG 5 cut(s) 150, 263, 851, 1010, 1166
BseDI CCNNGG 5 cut(s) 387, 849, 1164, 1473, 1587
BseGI GGATG 7 cut(s) 295, 336, 340, 537, 806, 899, 1234
BseLI CCNNNNNNNGG 1 cut(s) 693
BseMI GCAATG 2 cut(s) 811, 1111
BseMII CTCAG 3 cut(s) 579, 705, 1137
BseNI ACTGG 1 cut(s) 1596
BseXI GCAGC 1 cut(s) 1271
BshFI GGCC 2 cut(s) 686, 912
BshTI ACCGGT 1 cut(s) 37
BsiHKAI GWGCWC 1 cut(s) 283
BsiHKCI CYCGRG 1 cut(s) 19
BsiSI CCGG 2 cut(s) 38, 204
BslI CCNNNNNNNGG 1 cut(s) 693
BsmAI GTCTC 4 cut(s) 24, 150, 235, 642
BsnI GGCC 2 cut(s) 686, 912
Bso31I GGTCTC 2 cut(s) 24, 235
BsoBI CYCGRG 1 cut(s) 19
Bsp1286I GDGCHC 1 cut(s) 283
Bsp143I GATC 4 cut(s) 373, 409, 1294, 1414
BspACI CCGC 1 cut(s) 54
BspANI GGCC 2 cut(s) 686, 912
BspCNI CTCAG 3 cut(s) 580, 704, 1136
BspHI TCATGA 1 cut(s) 250
BspLI GGNNCC 1 cut(s) 385
BspPI GGATC 2 cut(s) 417, 1302
BspTI CTTAAG 1 cut(s) 482
BspTNI GGTCTC 2 cut(s) 24, 235
BsrDI GCAATG 2 cut(s) 811, 1111
BsrFI RCCGGY 2 cut(s) 37, 203
BsrI ACTGG 1 cut(s) 1596
BssAI RCCGGY 2 cut(s) 37, 203
BssECI CCNNGG 5 cut(s) 387, 849, 1164, 1473, 1587
BssMI GATC 4 cut(s) 373, 409, 1294, 1414
BssT1I CCWWGG 2 cut(s) 1473, 1587
Bst2UI CCWGG 5 cut(s) 150, 263, 851, 1010, 1166
Bst4CI ACNGT 6 cut(s) 136, 144, 458, 950, 1178, 1632
Bst6I CTCTTC 1 cut(s) 238
BstAFI CTTAAG 1 cut(s) 482
BstC8I GCNNGC 7 cut(s) 160, 537, 770, 1041, 1045, 1234, 1330
BstDEI CTNAG 5 cut(s) 559, 588, 691, 793, 1123
BstF5I GGATG 7 cut(s) 295, 336, 340, 537, 806, 899, 1234
BstH2I RGCGCY 1 cut(s) 1308
BstHHI GCGC 1 cut(s) 1307
BstKTI GATC 4 cut(s) 376, 412, 1297, 1417
BstMAI GTCTC 4 cut(s) 24, 150, 235, 642
BstMBI GATC 4 cut(s) 373, 409, 1294, 1414
BstMWI GCNNNNNNNGC 3 cut(s) 845, 1049, 1256
BstNI CCWGG 5 cut(s) 150, 263, 851, 1010, 1166
BstNSI RCATGY 2 cut(s) 822, 966
BstPAI GACNNNNGTC 1 cut(s) 1179
BstSCI CCNGG 5 cut(s) 148, 261, 849, 1008, 1164
BstSFI CTRYAG 2 cut(s) 135, 1628
BstV1I GCAGC 1 cut(s) 1271
BstX2I RGATCY 3 cut(s) 409, 1294, 1414
BstYI RGATCY 3 cut(s) 409, 1294, 1414
BsuRI GGCC 2 cut(s) 686, 912
BtsCI GGATG 7 cut(s) 295, 336, 340, 537, 806, 899, 1234
BtsI GCAGTG 1 cut(s) 516
BtsIMutI CAGTG 3 cut(s) 132, 499, 516
Cac8I GCNNGC 7 cut(s) 160, 537, 770, 1041, 1045, 1234, 1330
CaiI CAGNNNCTG 3 cut(s) 401, 1058, 1322
CciI TCATGA 1 cut(s) 250
CfoI GCGC 1 cut(s) 1307
Cfr10I RCCGGY 2 cut(s) 37, 203
CseI GACGC 2 cut(s) 652, 1294
Csp6I GTAC 1 cut(s) 1061
CspAI ACCGGT 1 cut(s) 37
CviAII CATG 7 cut(s) 251, 464, 476, 596, 819, 963, 1431
CviQI GTAC 1 cut(s) 1061
DdeI CTNAG 5 cut(s) 559, 588, 691, 793, 1123
DpnI GATC 4 cut(s) 375, 411, 1296, 1416
DpnII GATC 4 cut(s) 373, 409, 1294, 1414
DraI TTTAAA 4 cut(s) 501, 681, 1338, 1557
DrdI GACNNNNNNGTC 1 cut(s) 38
DseDI GACNNNNNNGTC 1 cut(s) 38
Eam1104I CTCTTC 1 cut(s) 238
EarI CTCTTC 1 cut(s) 238
Eco130I CCWWGG 2 cut(s) 1473, 1587
Eco31I GGTCTC 2 cut(s) 24, 235
Eco32I GATATC 1 cut(s) 723
Eco57I CTGAAG 2 cut(s) 546, 1113
Eco88I CYCGRG 1 cut(s) 19
EcoRII CCWGG 5 cut(s) 148, 261, 849, 1008, 1164
EcoRV GATATC 1 cut(s) 723
EcoT14I CCWWGG 2 cut(s) 1473, 1587
EcoT22I ATGCAT 2 cut(s) 191, 964
ErhI CCWWGG 2 cut(s) 1473, 1587
FaeI CATG 7 cut(s) 254, 467, 479, 599, 822, 966, 1434
FalI AAGNNNNNCTT 2 cut(s) 557, 589
FatI CATG 7 cut(s) 250, 463, 475, 595, 818, 962, 1430
Fnu4HI GCNGC 1 cut(s) 1260
FokI GGATG 7 cut(s) 302, 343, 347, 544, 813, 906, 1241
Fsp4HI GCNGC 1 cut(s) 1260
FspBI CTAG 1 cut(s) 110
GlaI GCGC 1 cut(s) 1306
GluI GCNGC 1 cut(s) 1260
GsuI CTGGAG 5 cut(s) 132, 245, 529, 1160, 1344
HaeII RGCGCY 1 cut(s) 1308
HaeIII GGCC 2 cut(s) 686, 912
HapII CCGG 2 cut(s) 38, 204
HgaI GACGC 2 cut(s) 652, 1294
HhaI GCGC 1 cut(s) 1307
Hin1II CATG 7 cut(s) 254, 467, 479, 599, 822, 966, 1434
Hin6I GCGC 1 cut(s) 1305
HinP1I GCGC 1 cut(s) 1305
HindIII AAGCTT 4 cut(s) 776, 1045, 1657, 1688
HpaII CCGG 2 cut(s) 38, 204
HphI GGTGA 5 cut(s) 314, 403, 1040, 1153, 1615
Hpy166II GTNNAC 2 cut(s) 347, 1161
Hpy8I GTNNAC 2 cut(s) 347, 1161
HpyAV CCTTC 7 cut(s) 44, 321, 521, 884, 919, 1364, 1673
HpyCH4III ACNGT 6 cut(s) 136, 144, 458, 950, 1178, 1632
HpyCH4IV ACGT 1 cut(s) 1576
HpyF10VI GCNNNNNNNGC 3 cut(s) 845, 1049, 1256
HpyF3I CTNAG 5 cut(s) 559, 588, 691, 793, 1123
HpySE526I ACGT 1 cut(s) 1576
Hsp92II CATG 7 cut(s) 254, 467, 479, 599, 822, 966, 1434
HspAI GCGC 1 cut(s) 1305
Kzo9I GATC 4 cut(s) 373, 409, 1294, 1414
LmnI GCTCC 2 cut(s) 506, 1325
Lsp1109I GCAGC 1 cut(s) 1271
MaeI CTAG 1 cut(s) 110
MaeII ACGT 1 cut(s) 1576
MaeIII GTNAC 7 cut(s) 391, 458, 664, 808, 1028, 1168, 1603
MalI GATC 4 cut(s) 375, 411, 1296, 1416
MboI GATC 4 cut(s) 373, 409, 1294, 1414
MboII GAAGA 7 cut(s) 59, 90, 225, 404, 1081, 1414, 1579
MflI RGATCY 3 cut(s) 409, 1294, 1414
MhlI GDGCHC 1 cut(s) 283
MlyI GAGTC 2 cut(s) 1313, 1601
MmeI TCCRAC 2 cut(s) 549, 1078
Mph1103I ATGCAT 2 cut(s) 191, 964
MslI CAYNNNNRTG 1 cut(s) 492
MspCI CTTAAG 1 cut(s) 482
MspI CCGG 2 cut(s) 38, 204
MspR9I CCNGG 5 cut(s) 150, 263, 851, 1010, 1166
MvaI CCWGG 5 cut(s) 150, 263, 851, 1010, 1166
MwoI GCNNNNNNNGC 3 cut(s) 845, 1049, 1256
NdeII GATC 4 cut(s) 373, 409, 1294, 1414
NlaIII CATG 7 cut(s) 254, 467, 479, 599, 822, 966, 1434
NlaIV GGNNCC 1 cut(s) 385
NmuCI GTSAC 5 cut(s) 391, 458, 664, 1028, 1603
NsiI ATGCAT 2 cut(s) 191, 964
NspI RCATGY 2 cut(s) 822, 966
OliI CACNNNNGTG 1 cut(s) 492
PaeR7I CTCGAG 1 cut(s) 19
PagI TCATGA 1 cut(s) 250
PfoI TCCNGGA 1 cut(s) 148
PinAI ACCGGT 1 cut(s) 37
PkrI GCNGC 1 cut(s) 1261
PleI GAGTC 2 cut(s) 1313, 1600
PpsI GAGTC 2 cut(s) 1313, 1600
PshAI GACNNNNGTC 1 cut(s) 1179
PsiI TTATAA 1 cut(s) 450
Psp6I CCWGG 5 cut(s) 148, 261, 849, 1008, 1164
PspGI CCWGG 5 cut(s) 148, 261, 849, 1008, 1164
PspN4I GGNNCC 1 cut(s) 385
PstNI CAGNNNCTG 3 cut(s) 401, 1058, 1322
PsuI RGATCY 3 cut(s) 409, 1294, 1414
RsaI GTAC 1 cut(s) 1062
RsaNI GTAC 1 cut(s) 1061
RseI CAYNNNNRTG 1 cut(s) 492
SatI GCNGC 1 cut(s) 1260
Sau3AI GATC 4 cut(s) 373, 409, 1294, 1414
SchI GAGTC 2 cut(s) 1313, 1601
ScrFI CCNGG 5 cut(s) 150, 263, 851, 1010, 1166
SduI GDGCHC 1 cut(s) 283
SfcI CTRYAG 2 cut(s) 135, 1628
Sfr274I CTCGAG 1 cut(s) 19
SlaI CTCGAG 1 cut(s) 19
SmiMI CAYNNNNRTG 1 cut(s) 492
SmlI CTYRAG 3 cut(s) 19, 284, 482
SmoI CTYRAG 3 cut(s) 19, 284, 482
SsiI CCGC 1 cut(s) 54
SspMI CTAG 1 cut(s) 110
StyD4I CCNGG 5 cut(s) 148, 261, 849, 1008, 1164
StyI CCWWGG 2 cut(s) 1473, 1587
TaaI ACNGT 6 cut(s) 136, 144, 458, 950, 1178, 1632
TaiI ACGT 1 cut(s) 1579
TaqI TCGA 4 cut(s) 20, 428, 1663, 1685
TatI WGTACW 1 cut(s) 1060
TscAI CASTG 3 cut(s) 139, 499, 516
TseFI GTSAC 5 cut(s) 391, 458, 664, 1028, 1603
TseI GCWGC 1 cut(s) 1259
Tsp45I GTSAC 5 cut(s) 391, 458, 664, 1028, 1603
TspDTI ATGAA 6 cut(s) 237, 345, 584, 599, 726, 903
TspRI CASTG 3 cut(s) 139, 499, 516
Vha464I CTTAAG 1 cut(s) 482
XapI RAATTY 4 cut(s) 991, 1072, 1438, 1522
XceI RCATGY 2 cut(s) 822, 966
XhoI CTCGAG 1 cut(s) 19
XspI CTAG 1 cut(s) 110
Zsp2I ATGCAT 2 cut(s) 191, 964
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.