FvH4_1g04230

The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0001510
Physical Location & Seq
Reverse (-)
2239678 .. 2243647
3970 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g04230.t2

Sequence Viewer

Length: 1509 bp
ATGCTCAAGCAAATTTTGAGCAAGTTCCCTCGCAAGCCAAAATCAGAGTCACTAGATTCCGCTGGGAGTGACTCTGGCAACAATAGTCCAATATTGGGAAGTGGTTTTCAATGTACAATCGGTGGGAGTTCTCTTACAAGCAAATTAAATGTTGTCAAGCGGGTGTCTTCAGCTGTTTTCCCAGCAAGCATTGCAGCTGGAGCAGAGGTAGTGGAACCTCATCTGTCTTTCAAAGAGGTTTCAAATCCACAGAAGCAGAATCTGTTTATCAGTAAGTTGAACGTTTGCTGCGAGATCATTGATTCTAGTGATCCAGTTAAGCAAGATCTTAAGCGTGAAACCTTGATTGAACTAGTTGATTTTGTTTCTTCTGGATCTGCAAAGTTTACTGAACCAGCAATATCTGCCATGTGTAAAATGTGTGCAACCAACCTGTTTAGAGTTTTCCCACCCAAGTACCGATCTAATTTTACTGGTGGTGAAACTGAAGATGAAGAACCAATGTTTGATCCTGCTTGGTCACATCTGCAAATTGTGTATGATCTACTTGTTCGGTTTATCAGTTACAGTTCGCTTGACATAAAGGTGGCGAAAAAGCATGTAGATCATTCTTTTATCGTGCGATTACTTGACCTCTTTGATTCTGAGGACCCAAGAGAAAGAGACTGCTTGAAAACTATTCTGCATAGGATCTATGGAAAATTCATGGTGCACAGACCTTTTATAAGGAAAGCTGTCAGCAATATCATATATCGTTTTGTCTTTGAAACTGAAAGGCATAATGGTATGTCTGAGCTGTTGGAGATTTTCGGGAGTGTAATTAGTGGGTTTGCTTTGCCATTGAAGGAAGAGCACAAGATATTTTTGTGGAGGGCTCTGATTCCTCTACACAAACCAAAATCAGTGGGTATTTATCACCAGCAATTGACATATTGCGTTGTACAGTTTATAGAGAAGGACCCAAAGTTGGCTAGTACTGTAATAAAGGGGTTGTTGAAATATTGGCCAGTAACAAATAGCCAGAAGGAGTTGATGTTTCTAAGTGAGTTAGAAGAGGTTTTGGAAATGACTAGCATGGACGAGTTCCAAAAGATCATGGTCCCTCTGTTCCGAAGAATCAGATGCTGCCTCAATAGTTCCCATTACCAGGTGGCTGAGCGAGCGCACTTGTTATGGAACAATGAACACATCCTTAACCTTATTGGGCATAACCGACAGGTGATTTTGCCACTTGTCTTCCCAGCTATTGAGCGAAATACCCAAAACCACTGGAATCAAGCAGTACTCAACCTGACAATGAATGTGAGGAAGATGTTTTGTGAGATGGATGAGGAGCTGGTACTTGCCTGCCAATGTAAATTGGAGGAGGAACATATTAAGTCAAATTCTGCAGCCGAGAAGAGAAGGATAACATGGGAACGCTTGGAAACTGCTGCTGGCGTCCAACCTGTTGTAGCTTGTAACTTTTTAGCTCCCATCAAGCCTTCTACTTGTCCTGTTGTCTGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03015 ko04071 ko04113 ko04114 ko04151 ko04152 ko04261 ko04728 ko05165 map03015 map04071 map04113 map04114 map04151 map04152 map04261 map04728 map05165
Pfam Domains
Protein Families
B56

Protein Analysis

503

Amino Acids

57.19

Weight (kDa)

8.1

Isoelectric Point (pI)

48.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B56 PF01603 76 - 479 2e-172 Protein phosphatase 2A regulatory B subunit (B56 family)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 725
AciI CCGC 2 cut(s) 60, 160
AclI AACGTT 1 cut(s) 282
AclWI GGATC 4 cut(s) 305, 382, 503, 698
AcoI YGGCCR 1 cut(s) 1004
AcsI RAATTY 3 cut(s) 12, 701, 1384
AcuI CTGAAG 2 cut(s) 153, 507
AcyI GRCGYC 1 cut(s) 1440
AfaI GTAC 6 cut(s) 115, 458, 942, 976, 1284, 1341
AfiI CCNNNNNNNGG 3 cut(s) 95, 967, 1147
AflII CTTAAG 1 cut(s) 329
AgsI TTSAA 9 cut(s) 110, 232, 243, 280, 350, 673, 767, 844, 997
AhlI ACTAGT 1 cut(s) 352
AjnI CCWGG 1 cut(s) 1146
AluBI AGCT 8 cut(s) 173, 197, 734, 796, 1244, 1336, 1457, 1472
AluI AGCT 8 cut(s) 173, 197, 734, 796, 1244, 1336, 1457, 1472
Alw21I GWGCWC 2 cut(s) 714, 855
Alw26I GTCTC 1 cut(s) 657
Alw44I GTGCAC 1 cut(s) 710
AlwI GGATC 4 cut(s) 305, 382, 503, 698
AlwNI CAGNNNCTG 2 cut(s) 262, 1125
AoxI GGCC 1 cut(s) 1004
ApaLI GTGCAC 1 cut(s) 710
ApeKI GCWGC 5 cut(s) 194, 288, 1125, 1391, 1433
ApoI RAATTY 3 cut(s) 12, 701, 1384
ArsI GACNNNNNNTTYG 2 cut(s) 32, 64
AspLEI GCGC 1 cut(s) 1165
AspS9I GGNCC 3 cut(s) 649, 958, 1099
AsuHPI GGTGA 3 cut(s) 491, 908, 1231
AvaII GGWCC 3 cut(s) 649, 958, 1099
BaeGI GKGCMC 1 cut(s) 714
BaeI ACNNNNGTAYC 2 cut(s) 440, 473
BalI TGGCCA 1 cut(s) 1006
BanII GRGCYC 1 cut(s) 877
BbsI GAAGAC 2 cut(s) 159, 1228
Bbv12I GWGCWC 2 cut(s) 714, 855
BbvI GCAGC 5 cut(s) 206, 275, 1112, 1403, 1420
BccI CCATC 2 cut(s) 1318, 1484
BciT130I CCWGG 1 cut(s) 1148
BcoDI GTCTC 1 cut(s) 657
BcuI ACTAGT 1 cut(s) 352
BfaI CTAG 6 cut(s) 53, 306, 353, 972, 1071, 1507
BfmI CTRYAG 1 cut(s) 1389
BfrI CTTAAG 1 cut(s) 329
BglII AGATCT 1 cut(s) 325
BisI GCNGC 5 cut(s) 195, 289, 1126, 1392, 1434
BlpI GCTNAGC 1 cut(s) 1155
BlsI GCNGC 5 cut(s) 196, 290, 1127, 1393, 1435
BmcAI AGTACT 2 cut(s) 976, 1284
Bme1390I CCNGG 1 cut(s) 1148
Bme18I GGWCC 3 cut(s) 649, 958, 1099
BmgT120I GGNCC 3 cut(s) 649, 958, 1099
BmiI GGNNCC 4 cut(s) 216, 651, 960, 1101
BmrFI CCNGG 1 cut(s) 1148
BmsI GCATC 1 cut(s) 1112
BpiI GAAGAC 2 cut(s) 159, 1228
BpmI CTGGAG 1 cut(s) 219
Bpu1102I GCTNAGC 1 cut(s) 1155
BsaHI GRCGYC 1 cut(s) 1440
BsaXI ACNNNNNCTCC 4 cut(s) 192, 222, 1019, 1049
Bsc4I CCNNNNNNNGG 3 cut(s) 95, 967, 1147
Bse1I ACTGG 4 cut(s) 314, 478, 1007, 1274
Bse3DI GCAATG 1 cut(s) 189
BseBI CCWGG 1 cut(s) 1148
BseGI GGATG 2 cut(s) 1188, 1333
BseLI CCNNNNNNNGG 3 cut(s) 95, 967, 1147
BseMI GCAATG 1 cut(s) 189
BseMII CTCAG 3 cut(s) 636, 783, 1146
BseNI ACTGG 4 cut(s) 314, 478, 1007, 1274
BseRI GAGGAG 2 cut(s) 1346, 1379
BseSI GKGCMC 1 cut(s) 714
BseXI GCAGC 5 cut(s) 206, 275, 1112, 1403, 1420
BseYI CCCAGC 3 cut(s) 62, 181, 1240
BshFI GGCC 1 cut(s) 1006
BsiHKAI GWGCWC 2 cut(s) 714, 855
BslFI GGGAC 1 cut(s) 1085
BslI CCNNNNNNNGG 3 cut(s) 95, 967, 1147
BsmAI GTCTC 1 cut(s) 657
BsmFI GGGAC 1 cut(s) 1085
BsnI GGCC 1 cut(s) 1006
Bsp1286I GDGCHC 3 cut(s) 714, 855, 877
Bsp1407I TGTACA 2 cut(s) 113, 940
Bsp1720I GCTNAGC 1 cut(s) 1155
BspACI CCGC 2 cut(s) 60, 160
BspANI GGCC 1 cut(s) 1006
BspCNI CTCAG 3 cut(s) 637, 784, 1147
BspLI GGNNCC 4 cut(s) 216, 651, 960, 1101
BspMAI CTGCAG 1 cut(s) 1393
BspPI GGATC 4 cut(s) 305, 382, 503, 698
BspQI GCTCTTC 1 cut(s) 843
BspTI CTTAAG 1 cut(s) 329
BsrDI GCAATG 1 cut(s) 189
BsrGI TGTACA 2 cut(s) 113, 940
BsrI ACTGG 4 cut(s) 314, 478, 1007, 1274
BssNI GRCGYC 1 cut(s) 1440
Bst2UI CCWGG 1 cut(s) 1148
Bst4CI ACNGT 3 cut(s) 569, 945, 979
Bst6I CTCTTC 3 cut(s) 843, 1047, 1394
BstACI GRCGYC 1 cut(s) 1440
BstAFI CTTAAG 1 cut(s) 329
BstAPI GCANNNNNTGC 2 cut(s) 191, 404
BstAUI TGTACA 2 cut(s) 113, 940
BstC8I GCNNGC 5 cut(s) 35, 187, 1161, 1348, 1438
BstDEI CTNAG 4 cut(s) 645, 792, 1040, 1155
BstF5I GGATG 2 cut(s) 1188, 1333
BstHHI GCGC 1 cut(s) 1165
BstMAI GTCTC 1 cut(s) 657
BstMWI GCNNNNNNNGC 4 cut(s) 191, 200, 404, 1160
BstNI CCWGG 1 cut(s) 1148
BstNSI RCATGY 1 cut(s) 602
BstSCI CCNGG 1 cut(s) 1146
BstSFI CTRYAG 1 cut(s) 1389
BstSLI GKGCMC 1 cut(s) 714
BstV1I GCAGC 5 cut(s) 206, 275, 1112, 1403, 1420
BstV2I GAAGAC 2 cut(s) 159, 1228
BstX2I RGATCY 3 cut(s) 325, 374, 690
BstYI RGATCY 3 cut(s) 325, 374, 690
BsuRI GGCC 1 cut(s) 1006
BtsCI GGATG 2 cut(s) 1188, 1333
BtsIMutI CAGTG 2 cut(s) 909, 1267
Cac8I GCNNGC 5 cut(s) 35, 187, 1161, 1348, 1438
CaiI CAGNNNCTG 2 cut(s) 262, 1125
CfoI GCGC 1 cut(s) 1165
Cfr13I GGNCC 3 cut(s) 649, 958, 1099
CseI GACGC 1 cut(s) 1429
CsiI ACCWGGT 1 cut(s) 1146
Csp6I GTAC 6 cut(s) 114, 457, 941, 975, 1283, 1340
CspCI CAANNNNNGTGG 2 cut(s) 885, 920
CviAII CATG 6 cut(s) 409, 599, 706, 1075, 1096, 1413
CviQI GTAC 6 cut(s) 114, 457, 941, 975, 1283, 1340
DdeI CTNAG 4 cut(s) 645, 792, 1040, 1155
EaeI YGGCCR 1 cut(s) 1004
Eam1104I CTCTTC 3 cut(s) 843, 1047, 1394
EarI CTCTTC 3 cut(s) 843, 1047, 1394
Eco24I GRGCYC 1 cut(s) 877
Eco47I GGWCC 3 cut(s) 649, 958, 1099
Eco57I CTGAAG 2 cut(s) 153, 507
EcoO109I RGGNCCY 2 cut(s) 649, 958
EcoRII CCWGG 1 cut(s) 1146
EcoT38I GRGCYC 1 cut(s) 877
FaeI CATG 6 cut(s) 412, 602, 709, 1078, 1099, 1416
FaqI GGGAC 1 cut(s) 1085
FatI CATG 6 cut(s) 408, 598, 705, 1074, 1095, 1412
FauI CCCGC 1 cut(s) 153
Fnu4HI GCNGC 5 cut(s) 195, 289, 1126, 1392, 1434
FokI GGATG 2 cut(s) 1175, 1340
FriOI GRGCYC 1 cut(s) 877
Fsp4HI GCNGC 5 cut(s) 195, 289, 1126, 1392, 1434
FspBI CTAG 6 cut(s) 53, 306, 353, 972, 1071, 1507
GlaI GCGC 1 cut(s) 1164
GluI GCNGC 5 cut(s) 195, 289, 1126, 1392, 1434
GsaI CCCAGC 3 cut(s) 66, 185, 1244
GsuI CTGGAG 1 cut(s) 219
HaeIII GGCC 1 cut(s) 1006
HgaI GACGC 1 cut(s) 1429
HhaI GCGC 1 cut(s) 1165
Hin1I GRCGYC 1 cut(s) 1440
Hin1II CATG 6 cut(s) 412, 602, 709, 1078, 1099, 1416
Hin6I GCGC 1 cut(s) 1163
HinP1I GCGC 1 cut(s) 1163
HinfI GANTC 9 cut(s) 47, 56, 71, 259, 302, 641, 880, 1116, 1273
HphI GGTGA 3 cut(s) 491, 908, 1231
Hpy166II GTNNAC 2 cut(s) 387, 712
Hpy188I TCNGA 6 cut(s) 46, 646, 793, 879, 1112, 1121
Hpy188III TCNNGA 2 cut(s) 372, 811
Hpy8I GTNNAC 2 cut(s) 387, 712
HpyAV CCTTC 5 cut(s) 838, 949, 1018, 1398, 1494
HpyCH4III ACNGT 3 cut(s) 569, 945, 979
HpyCH4IV ACGT 1 cut(s) 282
HpyCH4V TGCA 7 cut(s) 194, 380, 425, 529, 685, 712, 1391
HpyF10VI GCNNNNNNNGC 4 cut(s) 191, 200, 404, 1160
HpyF3I CTNAG 4 cut(s) 645, 792, 1040, 1155
HpySE526I ACGT 1 cut(s) 282
Hsp92I GRCGYC 1 cut(s) 1440
Hsp92II CATG 6 cut(s) 412, 602, 709, 1078, 1099, 1416
HspAI GCGC 1 cut(s) 1163
LguI GCTCTTC 1 cut(s) 843
LmnI GCTCC 3 cut(s) 200, 1333, 1477
Lsp1109I GCAGC 5 cut(s) 206, 275, 1112, 1403, 1420
LweI GCATC 1 cut(s) 1112
MabI ACCWGGT 1 cut(s) 1146
MaeI CTAG 6 cut(s) 53, 306, 353, 972, 1071, 1507
MaeII ACGT 1 cut(s) 282
MaeIII GTNAC 6 cut(s) 48, 68, 519, 563, 1009, 1460
MfeI CAATTG 1 cut(s) 923
MflI RGATCY 3 cut(s) 325, 374, 690
MhlI GDGCHC 3 cut(s) 714, 855, 877
MlsI TGGCCA 1 cut(s) 1006
MluCI AATT 9 cut(s) 12, 143, 466, 531, 701, 819, 923, 1358, 1384
MluNI TGGCCA 1 cut(s) 1006
MlyI GAGTC 2 cut(s) 56, 65
MmeI TCCRAC 2 cut(s) 780, 1468
Mox20I TGGCCA 1 cut(s) 1006
MscI TGGCCA 1 cut(s) 1006
MseI TTAA 5 cut(s) 146, 318, 330, 1194, 1377
MslI CAYNNNNRTG 1 cut(s) 584
Msp20I TGGCCA 1 cut(s) 1006
MspA1I CMGCKG 3 cut(s) 62, 173, 197
MspCI CTTAAG 1 cut(s) 329
MspR9I CCNGG 1 cut(s) 1148
MunI CAATTG 1 cut(s) 923
MvaI CCWGG 1 cut(s) 1148
MwoI GCNNNNNNNGC 4 cut(s) 191, 200, 404, 1160
NlaIII CATG 6 cut(s) 412, 602, 709, 1078, 1099, 1416
NlaIV GGNNCC 4 cut(s) 216, 651, 960, 1101
NmeAIII GCCGAG 1 cut(s) 1420
NmuCI GTSAC 3 cut(s) 48, 68, 519
NspI RCATGY 1 cut(s) 602
PciSI GCTCTTC 1 cut(s) 843
PcsI WCGNNNNNNNCGW 1 cut(s) 288
PfeI GAWTC 7 cut(s) 56, 259, 302, 641, 880, 1116, 1273
PkrI GCNGC 5 cut(s) 196, 290, 1127, 1393, 1435
PleI GAGTC 2 cut(s) 55, 65
PpsI GAGTC 2 cut(s) 55, 65
PpuMI RGGWCCY 2 cut(s) 649, 958
PsiI TTATAA 1 cut(s) 725
Psp1406I AACGTT 1 cut(s) 282
Psp5II RGGWCCY 2 cut(s) 649, 958
Psp6I CCWGG 1 cut(s) 1146
PspFI CCCAGC 3 cut(s) 62, 181, 1240
PspGI CCWGG 1 cut(s) 1146
PspN4I GGNNCC 4 cut(s) 216, 651, 960, 1101
PspPI GGNCC 3 cut(s) 649, 958, 1099
PspPPI RGGWCCY 2 cut(s) 649, 958
PstI CTGCAG 1 cut(s) 1393
PstNI CAGNNNCTG 2 cut(s) 262, 1125
PsuI RGATCY 3 cut(s) 325, 374, 690
PvuII CAGCTG 2 cut(s) 173, 197
RsaI GTAC 6 cut(s) 115, 458, 942, 976, 1284, 1341
RsaNI GTAC 6 cut(s) 114, 457, 941, 975, 1283, 1340
RseI CAYNNNNRTG 1 cut(s) 584
SapI GCTCTTC 1 cut(s) 843
SaqAI TTAA 5 cut(s) 146, 318, 330, 1194, 1377
SatI GCNGC 5 cut(s) 195, 289, 1126, 1392, 1434
Sau96I GGNCC 3 cut(s) 649, 958, 1099
ScaI AGTACT 2 cut(s) 976, 1284
SchI GAGTC 2 cut(s) 56, 65
ScrFI CCNGG 1 cut(s) 1148
SduI GDGCHC 3 cut(s) 714, 855, 877
SexAI ACCWGGT 1 cut(s) 1146
SfaNI GCATC 1 cut(s) 1112
SfcI CTRYAG 1 cut(s) 1389
SinI GGWCC 3 cut(s) 649, 958, 1099
SmiMI CAYNNNNRTG 1 cut(s) 584
SmlI CTYRAG 2 cut(s) 5, 329
SmoI CTYRAG 2 cut(s) 5, 329
SpeI ACTAGT 1 cut(s) 352
Sse9I AATT 9 cut(s) 12, 143, 466, 531, 701, 819, 923, 1358, 1384
SsiI CCGC 2 cut(s) 60, 160
SspI AATATT 2 cut(s) 93, 1001
SspMI CTAG 6 cut(s) 53, 306, 353, 972, 1071, 1507
StyD4I CCNGG 1 cut(s) 1146
TaaI ACNGT 3 cut(s) 569, 945, 979
TaiI ACGT 1 cut(s) 285
TasI AATT 9 cut(s) 12, 143, 466, 531, 701, 819, 923, 1358, 1384
TatI WGTACW 4 cut(s) 113, 940, 974, 1282
TfiI GAWTC 7 cut(s) 56, 259, 302, 641, 880, 1116, 1273
Tru1I TTAA 5 cut(s) 146, 318, 330, 1194, 1377
Tru9I TTAA 5 cut(s) 146, 318, 330, 1194, 1377
TscAI CASTG 2 cut(s) 909, 1274
TseFI GTSAC 3 cut(s) 48, 68, 519
TseI GCWGC 5 cut(s) 194, 288, 1125, 1391, 1433
Tsp45I GTSAC 3 cut(s) 48, 68, 519
TspDTI ATGAA 4 cut(s) 507, 694, 1197, 1313
TspRI CASTG 2 cut(s) 909, 1274
Vha464I CTTAAG 1 cut(s) 329
VneI GTGCAC 1 cut(s) 710
VpaK11BI GGWCC 3 cut(s) 649, 958, 1099
XapI RAATTY 3 cut(s) 12, 701, 1384
XceI RCATGY 1 cut(s) 602
XspI CTAG 6 cut(s) 53, 306, 353, 972, 1071, 1507
ZrmI AGTACT 2 cut(s) 976, 1284
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.