Rh2DG048800

Aux IAA proteins are short-lived transcriptional factors that function as repressors of early auxin response genes at low auxin concentrations

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0001510
Physical Location & Seq
Reverse (-)
3482593 .. 3483490
898 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG048800.1

Sequence Viewer

Length: 672 bp
ATGTACCGACAGACGGCACGAACTTCTAATCTGCTGCTGCCAAAAAATTCAAGCAACCGTAGCAGAGTTGCGCGCGGGTGTGGATCTGATACGTACGTACCAGTTAAGAACAAGGAGCAAATCCTACGCCGTATTTACACGTACTGGGCTCGCCGGAAAGCTGACGTGAACTTGTGGAATATGACGATGAATTACAAGGACACTGAGCTGACCTTGGGATTACCTGGTGGTGGAAGCCGTGGTTGTTCAACAGGTGTAGTGGTCTTCGGAGAGTTGATGAAGAGCTCGATGAAACGTGGGTTCATGGATACCGATGATGATCTCAACAACCTTGACCTGGAAAGCTCTAATGGCGAGCCTCGTGGCCAAGCTAAATTGGATGATGGGCCGTCGATGGAAGCAGCTAAGTACTCTTCCAGCAGTACCAGTACTACTGCCGTGAAAGCCCCAACTGCAAAGGGTCAAGTAGTTGGGTGGCCGCCGGTGAGATCGACGAGAAATAAGGCCTTGGACTCGAAGAACTTGAAGAACAACAGCAGCAGTAAATATGTGAAAGTGGGTGCTGATGGAGCTCCTTACCTGCGGAAAGTAGATTTAGAGATGTATAAAAGTTACGAGGAGCTATTAACAGCCTTGGAAAAAATTTTCACTTCCTTCACCATAAACGGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0009719 GO:0009725 GO:0009733 GO:0010033 GO:0042221 GO:0050896
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
AUX_IAA

Protein Analysis

223

Amino Acids

24.65

Weight (kDa)

9.61

Isoelectric Point (pI)

39.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AUX_IAA PF02309 67 - 221 1.2e-30 AUX/IAA family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 588
AccII CGCG 2 cut(s) 73, 75
AciI CCGC 3 cut(s) 75, 479, 583
AclWI GGATC 1 cut(s) 91
AcoI YGGCCR 2 cut(s) 364, 476
AcsI RAATTY 2 cut(s) 46, 642
AfaI GTAC 7 cut(s) 5, 95, 99, 143, 410, 424, 430
AfiI CCNNNNNNNGG 3 cut(s) 13, 230, 337
AflIII ACRYGT 1 cut(s) 138
AgsI TTSAA 3 cut(s) 51, 249, 526
AjiI CACGTC 1 cut(s) 166
AjnI CCWGG 2 cut(s) 223, 336
AluBI AGCT 8 cut(s) 161, 208, 285, 345, 371, 404, 572, 622
AluI AGCT 8 cut(s) 161, 208, 285, 345, 371, 404, 572, 622
Alw21I GWGCWC 2 cut(s) 287, 574
AlwI GGATC 1 cut(s) 91
AoxI GGCC 4 cut(s) 364, 386, 476, 504
ApeKI GCWGC 4 cut(s) 34, 37, 401, 537
ApoI RAATTY 2 cut(s) 46, 642
AspLEI GCGC 2 cut(s) 73, 75
AspS9I GGNCC 1 cut(s) 386
AsuHPI GGTGA 2 cut(s) 496, 649
BaeI ACNNNNGTAYC 4 cut(s) 81, 81, 114, 114
BalI TGGCCA 1 cut(s) 366
BanII GRGCYC 3 cut(s) 151, 287, 574
BauI CACGAG 1 cut(s) 360
BbsI GAAGAC 1 cut(s) 256
Bbv12I GWGCWC 2 cut(s) 287, 574
BbvI GCAGC 4 cut(s) 21, 24, 413, 549
BccI CCATC 3 cut(s) 377, 388, 560
BceAI ACGGC 5 cut(s) 30, 114, 222, 373, 422
BciT130I CCWGG 2 cut(s) 225, 338
BciVI GTATCC 1 cut(s) 301
BfuAI ACCTGC 1 cut(s) 588
BfuI GTATCC 1 cut(s) 301
BisI GCNGC 5 cut(s) 35, 38, 402, 479, 538
BlsI GCNGC 5 cut(s) 36, 39, 403, 480, 539
BmcAI AGTACT 2 cut(s) 410, 430
Bme1390I CCNGG 2 cut(s) 225, 338
BmgBI CACGTC 1 cut(s) 166
BmgT120I GGNCC 1 cut(s) 386
BmrFI CCNGG 2 cut(s) 225, 338
BmrI ACTGGG 1 cut(s) 154
BmuI ACTGGG 1 cut(s) 154
BpiI GAAGAC 1 cut(s) 256
BsaAI YACGTR 3 cut(s) 93, 97, 141
BsaBI GATNNNNATC 1 cut(s) 318
BsaJI CCNNGG 4 cut(s) 213, 238, 507, 633
Bsc4I CCNNNNNNNGG 3 cut(s) 13, 230, 337
Bse118I RCCGGY 1 cut(s) 481
Bse1I ACTGG 3 cut(s) 101, 149, 426
Bse8I GATNNNNATC 1 cut(s) 318
BseBI CCWGG 2 cut(s) 225, 338
BseDI CCNNGG 4 cut(s) 213, 238, 507, 633
BseGI GGATG 1 cut(s) 385
BseJI GATNNNNATC 1 cut(s) 318
BseLI CCNNNNNNNGG 3 cut(s) 13, 230, 337
BseMII CTCAG 1 cut(s) 195
BseNI ACTGG 3 cut(s) 101, 149, 426
BsePI GCGCGC 1 cut(s) 71
BseRI GAGGAG 1 cut(s) 632
BseXI GCAGC 4 cut(s) 21, 24, 413, 549
Bsh1236I CGCG 2 cut(s) 73, 75
BshFI GGCC 4 cut(s) 366, 388, 478, 506
BsiHKAI GWGCWC 2 cut(s) 287, 574
BsiSI CCGG 2 cut(s) 154, 482
BsiWI CGTACG 1 cut(s) 93
BslI CCNNNNNNNGG 3 cut(s) 13, 230, 337
BsnI GGCC 4 cut(s) 366, 388, 478, 506
Bsp1286I GDGCHC 3 cut(s) 151, 287, 574
Bsp143I GATC 3 cut(s) 83, 319, 488
BspACI CCGC 3 cut(s) 75, 479, 583
BspANI GGCC 4 cut(s) 366, 388, 478, 506
BspCNI CTCAG 1 cut(s) 196
BspFNI CGCG 2 cut(s) 73, 75
BspMI ACCTGC 1 cut(s) 588
BspPI GGATC 1 cut(s) 91
BspQI GCTCTTC 1 cut(s) 275
BsrFI RCCGGY 1 cut(s) 481
BsrI ACTGG 3 cut(s) 101, 149, 426
BssAI RCCGGY 1 cut(s) 481
BssECI CCNNGG 4 cut(s) 213, 238, 507, 633
BssHII GCGCGC 1 cut(s) 71
BssMI GATC 3 cut(s) 83, 319, 488
BssSI CACGAG 1 cut(s) 360
BssT1I CCWWGG 3 cut(s) 213, 507, 633
Bst2BI CACGAG 1 cut(s) 360
Bst2UI CCWGG 2 cut(s) 225, 338
Bst4CI ACNGT 2 cut(s) 59, 668
Bst6I CTCTTC 2 cut(s) 275, 418
BstBAI YACGTR 3 cut(s) 93, 97, 141
BstC8I GCNNGC 3 cut(s) 73, 151, 356
BstDEI CTNAG 2 cut(s) 204, 405
BstDSI CCRYGG 1 cut(s) 238
BstF5I GGATG 1 cut(s) 385
BstFNI CGCG 2 cut(s) 73, 75
BstHHI GCGC 2 cut(s) 73, 75
BstKTI GATC 3 cut(s) 86, 322, 491
BstMBI GATC 3 cut(s) 83, 319, 488
BstMWI GCNNNNNNNGC 5 cut(s) 60, 351, 443, 452, 569
BstNI CCWGG 2 cut(s) 225, 338
BstSCI CCNGG 2 cut(s) 223, 336
BstSNI TACGTA 2 cut(s) 93, 97
BstUI CGCG 2 cut(s) 73, 75
BstV1I GCAGC 4 cut(s) 21, 24, 413, 549
BstV2I GAAGAC 1 cut(s) 256
BstX2I RGATCY 1 cut(s) 83
BstYI RGATCY 1 cut(s) 83
BsuI GTATCC 1 cut(s) 301
BsuRI GGCC 4 cut(s) 366, 388, 478, 506
BtgI CCRYGG 1 cut(s) 238
BtrI CACGTC 1 cut(s) 166
BtsCI GGATG 1 cut(s) 385
BtsIMutI CAGTG 1 cut(s) 201
BveI ACCTGC 1 cut(s) 588
Cac8I GCNNGC 3 cut(s) 73, 151, 356
CfoI GCGC 2 cut(s) 73, 75
Cfr10I RCCGGY 1 cut(s) 481
Cfr13I GGNCC 1 cut(s) 386
CsiI ACCWGGT 1 cut(s) 223
Csp6I GTAC 7 cut(s) 4, 94, 98, 142, 409, 423, 429
CviAII CATG 1 cut(s) 304
CviQI GTAC 7 cut(s) 4, 94, 98, 142, 409, 423, 429
DdeI CTNAG 2 cut(s) 204, 405
DpnI GATC 3 cut(s) 85, 321, 490
DpnII GATC 3 cut(s) 83, 319, 488
EaeI YGGCCR 2 cut(s) 364, 476
Eam1104I CTCTTC 2 cut(s) 275, 418
EarI CTCTTC 2 cut(s) 275, 418
Ecl136II GAGCTC 2 cut(s) 285, 572
Eco105I TACGTA 2 cut(s) 93, 97
Eco130I CCWWGG 3 cut(s) 213, 507, 633
Eco147I AGGCCT 1 cut(s) 506
Eco24I GRGCYC 3 cut(s) 151, 287, 574
Eco53kI GAGCTC 2 cut(s) 285, 572
EcoICRI GAGCTC 2 cut(s) 285, 572
EcoRII CCWGG 2 cut(s) 223, 336
EcoT14I CCWWGG 3 cut(s) 213, 507, 633
EcoT38I GRGCYC 3 cut(s) 151, 287, 574
ErhI CCWWGG 3 cut(s) 213, 507, 633
FaeI CATG 1 cut(s) 307
FaiI YATR 5 cut(s) 182, 305, 549, 606, 662
FatI CATG 1 cut(s) 303
FauI CCCGC 1 cut(s) 68
Fnu4HI GCNGC 5 cut(s) 35, 38, 402, 479, 538
FokI GGATG 1 cut(s) 392
FriOI GRGCYC 3 cut(s) 151, 287, 574
Fsp4HI GCNGC 5 cut(s) 35, 38, 402, 479, 538
GlaI GCGC 2 cut(s) 72, 74
GluI GCNGC 5 cut(s) 35, 38, 402, 479, 538
HaeIII GGCC 4 cut(s) 366, 388, 478, 506
HapII CCGG 2 cut(s) 154, 482
HhaI GCGC 2 cut(s) 73, 75
Hin1II CATG 1 cut(s) 307
Hin6I GCGC 2 cut(s) 71, 73
HinP1I GCGC 2 cut(s) 71, 73
HinfI GANTC 1 cut(s) 512
HpaII CCGG 2 cut(s) 154, 482
HphI GGTGA 2 cut(s) 496, 649
Hpy166II GTNNAC 1 cut(s) 169
Hpy188I TCNGA 2 cut(s) 88, 269
Hpy8I GTNNAC 1 cut(s) 169
Hpy99I CGWCG 2 cut(s) 394, 496
HpyAV CCTTC 1 cut(s) 664
HpyCH4III ACNGT 2 cut(s) 59, 668
HpyCH4IV ACGT 5 cut(s) 92, 96, 140, 165, 295
HpyCH4V TGCA 1 cut(s) 455
HpyF10VI GCNNNNNNNGC 5 cut(s) 60, 351, 443, 452, 569
HpyF3I CTNAG 2 cut(s) 204, 405
HpySE526I ACGT 5 cut(s) 92, 96, 140, 165, 295
Hsp92II CATG 1 cut(s) 307
HspAI GCGC 2 cut(s) 71, 73
Kzo9I GATC 3 cut(s) 83, 319, 488
LguI GCTCTTC 1 cut(s) 275
LmnI GCTCC 4 cut(s) 115, 569, 577, 619
Lsp1109I GCAGC 4 cut(s) 21, 24, 413, 549
MabI ACCWGGT 1 cut(s) 223
MaeII ACGT 5 cut(s) 92, 96, 140, 165, 295
MaeIII GTNAC 1 cut(s) 611
MalI GATC 3 cut(s) 85, 321, 490
MboI GATC 3 cut(s) 83, 319, 488
MboII GAAGA 5 cut(s) 256, 292, 405, 529, 538
MflI RGATCY 1 cut(s) 83
MhlI GDGCHC 3 cut(s) 151, 287, 574
MlsI TGGCCA 1 cut(s) 366
MluCI AATT 4 cut(s) 46, 190, 374, 642
MluNI TGGCCA 1 cut(s) 366
MlyI GAGTC 1 cut(s) 506
MnlI CCTC 2 cut(s) 369, 610
Mox20I TGGCCA 1 cut(s) 366
MscI TGGCCA 1 cut(s) 366
MseI TTAA 2 cut(s) 105, 626
Msp20I TGGCCA 1 cut(s) 366
MspI CCGG 2 cut(s) 154, 482
MspR9I CCNGG 2 cut(s) 225, 338
MvaI CCWGG 2 cut(s) 225, 338
MvnI CGCG 2 cut(s) 73, 75
MwoI GCNNNNNNNGC 5 cut(s) 60, 351, 443, 452, 569
NdeII GATC 3 cut(s) 83, 319, 488
NlaIII CATG 1 cut(s) 307
PauI GCGCGC 1 cut(s) 71
PceI AGGCCT 1 cut(s) 506
PciSI GCTCTTC 1 cut(s) 275
Pfl23II CGTACG 1 cut(s) 93
PkrI GCNGC 5 cut(s) 36, 39, 403, 480, 539
PleI GAGTC 1 cut(s) 506
PpsI GAGTC 1 cut(s) 506
Ppu21I YACGTR 3 cut(s) 93, 97, 141
Psp124BI GAGCTC 2 cut(s) 287, 574
Psp6I CCWGG 2 cut(s) 223, 336
PspGI CCWGG 2 cut(s) 223, 336
PspLI CGTACG 1 cut(s) 93
PspPI GGNCC 1 cut(s) 386
PsuI RGATCY 1 cut(s) 83
PteI GCGCGC 1 cut(s) 71
RsaI GTAC 7 cut(s) 5, 95, 99, 143, 410, 424, 430
RsaNI GTAC 7 cut(s) 4, 94, 98, 142, 409, 423, 429
SacI GAGCTC 2 cut(s) 287, 574
SapI GCTCTTC 1 cut(s) 275
SaqAI TTAA 2 cut(s) 105, 626
SatI GCNGC 5 cut(s) 35, 38, 402, 479, 538
Sau3AI GATC 3 cut(s) 83, 319, 488
Sau96I GGNCC 1 cut(s) 386
ScaI AGTACT 2 cut(s) 410, 430
SchI GAGTC 1 cut(s) 506
ScrFI CCNGG 2 cut(s) 225, 338
SduI GDGCHC 3 cut(s) 151, 287, 574
SexAI ACCWGGT 1 cut(s) 223
SgrAI CRCCGGYG 1 cut(s) 481
SnaBI TACGTA 2 cut(s) 93, 97
Sse9I AATT 4 cut(s) 46, 190, 374, 642
SseBI AGGCCT 1 cut(s) 506
SsiI CCGC 3 cut(s) 75, 479, 583
SstI GAGCTC 2 cut(s) 287, 574
StuI AGGCCT 1 cut(s) 506
StyD4I CCNGG 2 cut(s) 223, 336
StyI CCWWGG 3 cut(s) 213, 507, 633
TaaI ACNGT 2 cut(s) 59, 668
TaiI ACGT 5 cut(s) 95, 99, 143, 168, 298
TaqI TCGA 4 cut(s) 287, 392, 491, 515
TasI AATT 4 cut(s) 46, 190, 374, 642
TatI WGTACW 2 cut(s) 408, 428
TauI GCSGC 1 cut(s) 481
Tru1I TTAA 2 cut(s) 105, 626
Tru9I TTAA 2 cut(s) 105, 626
TscAI CASTG 1 cut(s) 208
TseI GCWGC 4 cut(s) 34, 37, 401, 537
TspDTI ATGAA 4 cut(s) 203, 292, 293, 305
TspRI CASTG 1 cut(s) 208
XapI RAATTY 2 cut(s) 46, 642
ZrmI AGTACT 2 cut(s) 410, 430
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.