Rw2G004200

The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0001510
Physical Location & Seq
Forward (+)
4004944 .. 4008693
3750 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G004200.1

Sequence Viewer

Length: 1509 bp
ATGCTTAAGCAAATTCTGAGCAAATTCCCTCGCAAGCCAAAATCAGAGTCGCTAGATTCCACCGGGAGTGATTCTGGCAACAATAGTCCCAATTTGGGCAGTGGTTTCCAATGTACAATTGGTGGGAGTTCTCTTACAAGCAAATTAAATGTTGTCAAACGAGTGTCTTCAGCTGTTTTCCCAGCAAGCATTTCAGCTGGAGCAGAGGTAGTGGAGCCTCATCTGTCTTTCAAAGAGGTTTCAAATCCACAGAAGCAGAATCTGTTTATCAGTAAGTTGAATGTTTGCTGCGAGATCATTGATTCTAGCGATCCAGCTAAGCAAGATCTTAAGCGTGAAACGTTGATTGAACTTGTTGATTTTGTTTCTTCTGGATCTGCAAAGTTTAATGAACCAGCAATATCTGCAATGTGTAAAATGTGTGCAACCAACCTGTTTAGAGTTTTCCCACCTAAGTACCGATCTAATTTTACTGGTGGTGAAACCGAAGATGAAGAACCAATGTTTGATCCTGCTTGGTCACATCTGCAAATTGTGTATGATCTACTGGTTCGGTTTATCAGTTACAATTCGCTTGACATAAAGGTGGCGAAAAAGCATGTAGATCATTCTTTTATCGTACGATTACTTGACCTCTTTGATTCCGAGGACCCAAGAGAAAGAGACTGCTTGAAAACTATTCTGCATAGGATCTATGGAAAATTCATGGTGCATAGACCTTTTATACGAAAAGCTGTCAGCAATATCATCTATCGTTTTGTTTTTGAAACTGAAAGGCATAATGGTATGTCTGAGCTGTTGGAAATTTTTGGAAGTGTAATTAGCGGGTTTGCTTTGCCATTGAAGGAGGAGCACAAGATATTTTTGTGGAGGGCTCTGATTCCTCTACACAAACCAAAATCAGTGGGAATTTATCATCAGCAATTGACATATTGCGTTGTACAGTTTATAGAGAAGGACCCGAAGTTGGCTAGCACTGTTATAAAGGGATTGTTGAAGTACTGGCCAGTAACAAATAGCCAGAAGGAGTTGATGTTTCTAAGTGAGTTAGAAGAGGTTTTGGAAATGACTAGTATGGACGAGTTCCAAAAGATCATGGTCCCTTTGTTCCGAAGAATTAGATGCTGCCTCAATAGTTCCCATTACCAGGTGGCTGAGCGAGCTCACTTGCTATGGAACAATGAGCACATCCTTAACCTTATCGGGCATAACCGCCAGGTGATTTTGCCACTTGTCTTCCCAGCTATTGAGCGAAACACCCAAAACCACTGGAATCAAGCGGTGCTCAACCTGACAATGAATGTGAGGAAGATGTTTTGCGAGATGGATGAGGAGCTGGTACTTGCCTGCCAATGTAAGTTGGAGGAGGAACACATAAAGTCAAATTCTGCAGCCGAGAAGCGAAGGTTAACATGGGAACGCTTGGAAACTGCTGCTGGCGTTCAACCTGTTGTAGCTTGTAACTTTGTAGCTCCCATCAAGCCTTCCACTTGTCCTGTTGTCTGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03015 ko04071 ko04113 ko04114 ko04151 ko04152 ko04261 ko04728 ko05165 map03015 map04071 map04113 map04114 map04151 map04152 map04261 map04728 map05165
Pfam Domains
Protein Families
B56

Protein Analysis

502

Amino Acids

57.24

Weight (kDa)

8.1

Isoelectric Point (pI)

47.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B56 PF01603 76 - 479 2.3e-172 Protein phosphatase 2A regulatory B subunit (B56 family)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 983
AciI CCGC 3 cut(s) 825, 1213, 1280
AclI AACGTT 1 cut(s) 341
AclWI GGATC 4 cut(s) 305, 382, 503, 698
AcoI YGGCCR 1 cut(s) 1004
AcsI RAATTY 6 cut(s) 12, 23, 701, 804, 909, 1384
AcuI CTGAAG 1 cut(s) 153
AfaI GTAC 6 cut(s) 115, 458, 621, 942, 1001, 1341
AfiI CCNNNNNNNGG 3 cut(s) 95, 967, 1147
AflII CTTAAG 2 cut(s) 5, 329
AgsI TTSAA 9 cut(s) 232, 243, 280, 350, 673, 767, 844, 997, 1445
AhlI ACTAGT 1 cut(s) 1070
AjnI CCWGG 2 cut(s) 1146, 1215
Alw21I GWGCWC 4 cut(s) 855, 1165, 1188, 1287
Alw26I GTCTC 1 cut(s) 657
AlwI GGATC 4 cut(s) 305, 382, 503, 698
AlwNI CAGNNNCTG 1 cut(s) 262
AoxI GGCC 1 cut(s) 1004
ApeKI GCWGC 4 cut(s) 288, 1125, 1391, 1433
ApoI RAATTY 6 cut(s) 12, 23, 701, 804, 909, 1384
ArsI GACNNNNNNTTYG 2 cut(s) 32, 64
AspS9I GGNCC 3 cut(s) 649, 958, 1099
AsuC2I CCSGG 1 cut(s) 64
AsuHPI GGTGA 2 cut(s) 491, 1231
AsuNHI GCTAGC 1 cut(s) 971
AvaII GGWCC 3 cut(s) 649, 958, 1099
BaeI ACNNNNGTAYC 2 cut(s) 440, 473
BalI TGGCCA 1 cut(s) 1006
BanII GRGCYC 2 cut(s) 877, 1165
BbsI GAAGAC 2 cut(s) 159, 1228
Bbv12I GWGCWC 4 cut(s) 855, 1165, 1188, 1287
BbvI GCAGC 4 cut(s) 275, 1112, 1403, 1420
BccI CCATC 2 cut(s) 1318, 1484
BciT130I CCWGG 2 cut(s) 1148, 1217
BcnI CCSGG 1 cut(s) 64
BcoDI GTCTC 1 cut(s) 657
BcuI ACTAGT 1 cut(s) 1070
BfaI CTAG 4 cut(s) 53, 306, 972, 1071
BfmI CTRYAG 1 cut(s) 1389
BfrI CTTAAG 2 cut(s) 5, 329
BglII AGATCT 1 cut(s) 325
BisI GCNGC 4 cut(s) 289, 1126, 1392, 1434
BlpI GCTNAGC 2 cut(s) 318, 1155
BlsI GCNGC 4 cut(s) 290, 1127, 1393, 1435
BmcAI AGTACT 1 cut(s) 1001
Bme1390I CCNGG 3 cut(s) 64, 1148, 1217
Bme18I GGWCC 3 cut(s) 649, 958, 1099
BmgT120I GGNCC 3 cut(s) 649, 958, 1099
BmiI GGNNCC 4 cut(s) 216, 651, 960, 1101
BmrFI CCNGG 3 cut(s) 64, 1148, 1217
BmsI GCATC 1 cut(s) 1112
BmtI GCTAGC 1 cut(s) 975
BpiI GAAGAC 2 cut(s) 159, 1228
BpmI CTGGAG 1 cut(s) 219
Bpu1102I GCTNAGC 2 cut(s) 318, 1155
BpuMI CCSGG 1 cut(s) 64
BsaJI CCNNGG 1 cut(s) 645
BsaXI ACNNNNNCTCC 4 cut(s) 192, 222, 1019, 1049
Bsc4I CCNNNNNNNGG 3 cut(s) 95, 967, 1147
Bse1I ACTGG 5 cut(s) 478, 552, 1007, 1007, 1274
Bse3DI GCAATG 1 cut(s) 414
BseBI CCWGG 2 cut(s) 1148, 1217
BseDI CCNNGG 1 cut(s) 645
BseGI GGATG 2 cut(s) 1188, 1333
BseLI CCNNNNNNNGG 3 cut(s) 95, 967, 1147
BseMI GCAATG 1 cut(s) 414
BseMII CTCAG 3 cut(s) 8, 783, 1146
BseNI ACTGG 5 cut(s) 478, 552, 1007, 1007, 1274
BseRI GAGGAG 3 cut(s) 863, 1346, 1379
BseXI GCAGC 4 cut(s) 275, 1112, 1403, 1420
BseYI CCCAGC 2 cut(s) 181, 1240
BshFI GGCC 1 cut(s) 1006
BsiHKAI GWGCWC 4 cut(s) 855, 1165, 1188, 1287
BsiSI CCGG 1 cut(s) 63
BsiWI CGTACG 1 cut(s) 619
BslFI GGGAC 2 cut(s) 72, 1085
BslI CCNNNNNNNGG 3 cut(s) 95, 967, 1147
BsmAI GTCTC 1 cut(s) 657
BsmFI GGGAC 2 cut(s) 72, 1085
BsnI GGCC 1 cut(s) 1006
Bsp1286I GDGCHC 5 cut(s) 855, 877, 1165, 1188, 1287
Bsp1407I TGTACA 2 cut(s) 113, 940
Bsp1720I GCTNAGC 2 cut(s) 318, 1155
BspACI CCGC 3 cut(s) 825, 1213, 1280
BspANI GGCC 1 cut(s) 1006
BspCNI CTCAG 3 cut(s) 9, 784, 1147
BspLI GGNNCC 4 cut(s) 216, 651, 960, 1101
BspMAI CTGCAG 1 cut(s) 1393
BspOI GCTAGC 1 cut(s) 975
BspPI GGATC 4 cut(s) 305, 382, 503, 698
BspTI CTTAAG 2 cut(s) 5, 329
BsrDI GCAATG 1 cut(s) 414
BsrGI TGTACA 2 cut(s) 113, 940
BsrI ACTGG 5 cut(s) 478, 552, 1007, 1007, 1274
BssECI CCNNGG 1 cut(s) 645
Bst2UI CCWGG 2 cut(s) 1148, 1217
Bst4CI ACNGT 2 cut(s) 945, 979
Bst6I CTCTTC 1 cut(s) 1047
BstAFI CTTAAG 2 cut(s) 5, 329
BstAPI GCANNNNNTGC 1 cut(s) 404
BstAUI TGTACA 2 cut(s) 113, 940
BstC8I GCNNGC 6 cut(s) 35, 187, 973, 1161, 1348, 1438
BstDEI CTNAG 6 cut(s) 17, 318, 453, 792, 1040, 1155
BstF5I GGATG 2 cut(s) 1188, 1333
BstMAI GTCTC 1 cut(s) 657
BstMWI GCNNNNNNNGC 2 cut(s) 404, 1160
BstNI CCWGG 2 cut(s) 1148, 1217
BstNSI RCATGY 1 cut(s) 602
BstSCI CCNGG 3 cut(s) 62, 1146, 1215
BstSFI CTRYAG 1 cut(s) 1389
BstV1I GCAGC 4 cut(s) 275, 1112, 1403, 1420
BstV2I GAAGAC 2 cut(s) 159, 1228
BstX2I RGATCY 3 cut(s) 325, 374, 690
BstYI RGATCY 3 cut(s) 325, 374, 690
BsuRI GGCC 1 cut(s) 1006
BtsCI GGATG 2 cut(s) 1188, 1333
BtsI GCAGTG 1 cut(s) 106
BtsIMutI CAGTG 4 cut(s) 106, 909, 975, 1267
Cac8I GCNNGC 6 cut(s) 35, 187, 973, 1161, 1348, 1438
CaiI CAGNNNCTG 1 cut(s) 262
Cfr13I GGNCC 3 cut(s) 649, 958, 1099
CsiI ACCWGGT 1 cut(s) 1146
Csp6I GTAC 6 cut(s) 114, 457, 620, 941, 1000, 1340
CspCI CAANNNNNGTGG 2 cut(s) 885, 920
CviAII CATG 4 cut(s) 599, 706, 1096, 1413
CviQI GTAC 6 cut(s) 114, 457, 620, 941, 1000, 1340
DdeI CTNAG 6 cut(s) 17, 318, 453, 792, 1040, 1155
EaeI YGGCCR 1 cut(s) 1004
Eam1104I CTCTTC 1 cut(s) 1047
EarI CTCTTC 1 cut(s) 1047
Ecl136II GAGCTC 1 cut(s) 1163
Eco24I GRGCYC 2 cut(s) 877, 1165
Eco47I GGWCC 3 cut(s) 649, 958, 1099
Eco53kI GAGCTC 1 cut(s) 1163
Eco57I CTGAAG 1 cut(s) 153
EcoICRI GAGCTC 1 cut(s) 1163
EcoO109I RGGNCCY 2 cut(s) 649, 958
EcoRII CCWGG 2 cut(s) 1146, 1215
EcoT38I GRGCYC 2 cut(s) 877, 1165
FaeI CATG 4 cut(s) 602, 709, 1099, 1416
FaqI GGGAC 2 cut(s) 72, 1085
FatI CATG 4 cut(s) 598, 705, 1095, 1412
FauI CCCGC 1 cut(s) 818
Fnu4HI GCNGC 4 cut(s) 289, 1126, 1392, 1434
FokI GGATG 2 cut(s) 1175, 1340
FriOI GRGCYC 2 cut(s) 877, 1165
Fsp4HI GCNGC 4 cut(s) 289, 1126, 1392, 1434
FspBI CTAG 4 cut(s) 53, 306, 972, 1071
GluI GCNGC 4 cut(s) 289, 1126, 1392, 1434
GsaI CCCAGC 2 cut(s) 185, 1244
GsuI CTGGAG 1 cut(s) 219
HaeIII GGCC 1 cut(s) 1006
HapII CCGG 1 cut(s) 63
Hin1II CATG 4 cut(s) 602, 709, 1099, 1416
HincII GTYRAC 1 cut(s) 1410
HindII GTYRAC 1 cut(s) 1410
HinfI GANTC 8 cut(s) 47, 56, 71, 259, 302, 641, 880, 1273
HpaI GTTAAC 1 cut(s) 1410
HpaII CCGG 1 cut(s) 63
HphI GGTGA 2 cut(s) 491, 1231
Hpy166II GTNNAC 1 cut(s) 1410
Hpy188I TCNGA 6 cut(s) 18, 46, 646, 793, 879, 1112
Hpy188III TCNNGA 1 cut(s) 372
Hpy8I GTNNAC 1 cut(s) 1410
HpyAV CCTTC 5 cut(s) 838, 949, 1018, 1398, 1494
HpyCH4III ACNGT 2 cut(s) 945, 979
HpyCH4IV ACGT 1 cut(s) 341
HpyCH4V TGCA 7 cut(s) 380, 407, 425, 529, 685, 712, 1391
HpyF10VI GCNNNNNNNGC 2 cut(s) 404, 1160
HpyF3I CTNAG 6 cut(s) 17, 318, 453, 792, 1040, 1155
HpySE526I ACGT 1 cut(s) 341
Hsp92II CATG 4 cut(s) 602, 709, 1099, 1416
KspAI GTTAAC 1 cut(s) 1410
LmnI GCTCC 5 cut(s) 200, 214, 850, 1333, 1477
Lsp1109I GCAGC 4 cut(s) 275, 1112, 1403, 1420
LweI GCATC 1 cut(s) 1112
MabI ACCWGGT 1 cut(s) 1146
MaeI CTAG 4 cut(s) 53, 306, 972, 1071
MaeII ACGT 1 cut(s) 341
MaeIII GTNAC 4 cut(s) 519, 563, 1009, 1460
MboII GAAGA 8 cut(s) 159, 360, 500, 506, 1064, 1125, 1228, 1321
MfeI CAATTG 2 cut(s) 117, 923
MflI RGATCY 3 cut(s) 325, 374, 690
MhlI GDGCHC 5 cut(s) 855, 877, 1165, 1188, 1287
MlsI TGGCCA 1 cut(s) 1006
MluNI TGGCCA 1 cut(s) 1006
MlyI GAGTC 1 cut(s) 56
MmeI TCCRAC 2 cut(s) 780, 1341
Mox20I TGGCCA 1 cut(s) 1006
MscI TGGCCA 1 cut(s) 1006
MseI TTAA 6 cut(s) 6, 146, 330, 387, 1194, 1409
MslI CAYNNNNRTG 1 cut(s) 584
Msp20I TGGCCA 1 cut(s) 1006
MspA1I CMGCKG 2 cut(s) 173, 197
MspCI CTTAAG 2 cut(s) 5, 329
MspI CCGG 1 cut(s) 63
MspR9I CCNGG 3 cut(s) 64, 1148, 1217
MunI CAATTG 2 cut(s) 117, 923
MvaI CCWGG 2 cut(s) 1148, 1217
MwoI GCNNNNNNNGC 2 cut(s) 404, 1160
NciI CCSGG 1 cut(s) 64
NheI GCTAGC 1 cut(s) 971
NlaIII CATG 4 cut(s) 602, 709, 1099, 1416
NlaIV GGNNCC 4 cut(s) 216, 651, 960, 1101
NmeAIII GCCGAG 1 cut(s) 1420
NmuCI GTSAC 1 cut(s) 519
NspI RCATGY 1 cut(s) 602
PfeI GAWTC 7 cut(s) 56, 71, 259, 302, 641, 880, 1273
Pfl23II CGTACG 1 cut(s) 619
PkrI GCNGC 4 cut(s) 290, 1127, 1393, 1435
PleI GAGTC 1 cut(s) 55
PpsI GAGTC 1 cut(s) 55
PpuMI RGGWCCY 2 cut(s) 649, 958
PsiI TTATAA 1 cut(s) 983
Psp124BI GAGCTC 1 cut(s) 1165
Psp1406I AACGTT 1 cut(s) 341
Psp5II RGGWCCY 2 cut(s) 649, 958
Psp6I CCWGG 2 cut(s) 1146, 1215
PspFI CCCAGC 2 cut(s) 181, 1240
PspGI CCWGG 2 cut(s) 1146, 1215
PspLI CGTACG 1 cut(s) 619
PspN4I GGNNCC 4 cut(s) 216, 651, 960, 1101
PspPI GGNCC 3 cut(s) 649, 958, 1099
PspPPI RGGWCCY 2 cut(s) 649, 958
PstI CTGCAG 1 cut(s) 1393
PstNI CAGNNNCTG 1 cut(s) 262
PsuI RGATCY 3 cut(s) 325, 374, 690
PvuII CAGCTG 2 cut(s) 173, 197
RsaI GTAC 6 cut(s) 115, 458, 621, 942, 1001, 1341
RsaNI GTAC 6 cut(s) 114, 457, 620, 941, 1000, 1340
RseI CAYNNNNRTG 1 cut(s) 584
SacI GAGCTC 1 cut(s) 1165
SaqAI TTAA 6 cut(s) 6, 146, 330, 387, 1194, 1409
SatI GCNGC 4 cut(s) 289, 1126, 1392, 1434
Sau96I GGNCC 3 cut(s) 649, 958, 1099
ScaI AGTACT 1 cut(s) 1001
SchI GAGTC 1 cut(s) 56
ScrFI CCNGG 3 cut(s) 64, 1148, 1217
SduI GDGCHC 5 cut(s) 855, 877, 1165, 1188, 1287
SexAI ACCWGGT 1 cut(s) 1146
SfaNI GCATC 1 cut(s) 1112
SfcI CTRYAG 1 cut(s) 1389
SinI GGWCC 3 cut(s) 649, 958, 1099
SmiMI CAYNNNNRTG 1 cut(s) 584
SmlI CTYRAG 2 cut(s) 5, 329
SmoI CTYRAG 2 cut(s) 5, 329
SpeI ACTAGT 1 cut(s) 1070
SsiI CCGC 3 cut(s) 825, 1213, 1280
SspMI CTAG 4 cut(s) 53, 306, 972, 1071
SstI GAGCTC 1 cut(s) 1165
StyD4I CCNGG 3 cut(s) 62, 1146, 1215
TaaI ACNGT 2 cut(s) 945, 979
TaiI ACGT 1 cut(s) 344
TatI WGTACW 3 cut(s) 113, 940, 999
TfiI GAWTC 7 cut(s) 56, 71, 259, 302, 641, 880, 1273
Tru1I TTAA 6 cut(s) 6, 146, 330, 387, 1194, 1409
Tru9I TTAA 6 cut(s) 6, 146, 330, 387, 1194, 1409
TscAI CASTG 4 cut(s) 106, 909, 982, 1274
TseFI GTSAC 1 cut(s) 519
TseI GCWGC 4 cut(s) 288, 1125, 1391, 1433
Tsp45I GTSAC 1 cut(s) 519
TspDTI ATGAA 4 cut(s) 405, 507, 694, 1313
TspRI CASTG 4 cut(s) 106, 909, 982, 1274
Vha464I CTTAAG 2 cut(s) 5, 329
VpaK11BI GGWCC 3 cut(s) 649, 958, 1099
XapI RAATTY 6 cut(s) 12, 23, 701, 804, 909, 1384
XceI RCATGY 1 cut(s) 602
XcmI CCANNNNNNNNNTGG 1 cut(s) 116
XspI CTAG 4 cut(s) 53, 306, 972, 1071
ZrmI AGTACT 1 cut(s) 1001
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.