pycom02g03510

The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0001510
Physical Location & Seq
Reverse (-)
2336525 .. 2338171
1647 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g03510.2

Sequence Viewer

Length: 1509 bp
ATGCTGAAGCAAATCCTAAGCAAACTTCCTCGCAAGTCGTCAAAGTCTGATTCACTAGATTCTATAGGGAGTGATTCTGGCAGCAATACTTCCAATTTGGGGAGTGGCTTCCCATGTACAATTGGTGGGAGTTCTCTTACAAGCAAATTAAATGTTGTCAAGCGGGTGTCTTCAGCAGTTTTCCCATCAAGCATCGCAGCCGGAGCTGAGGCAGTGGAGCCCCATCTCTCCTTCAAGGAGGTTTCAAACCCACAGAAGCATAATCTATTTATCAGCAAGCTGAACCTTTGCTGTGAGTTCGTCGATTCCAGCGATCCAGTTAAGCAAGATCTCAAACGTGAAACACTGATAGAACTTGTTGATTATGTTTCTTCTGGGACTGTGAAGTTCACTGAACCAGCAATTTCTGCACTGTGTAAAATGTGTGCAGTCAACCTGTTTAGAGTTTTCCCACCTAAGTATCGATCTAATTTTACTGGGGGTGAAACAGAAGATGAAGAACCAATGTTTGATCCTGCTTGGTCATATCTGCAAATTGTGTATGATCTTCTGGTTCGGTTTATCAGTTATAGTTCACTTGATGTAAAGGTGGCAAAAAAGCATGTGGATCACTCTTTTATCGTAAAATTACTTGACCTCTTTGACTCTGAGGACCCAAGAGAAAGAGATTGTTTGAAAACCATTCTGCATAGGATTTATGGAAAGTTCATGGTACACAGGCCATTTATTCGGAAAGCTGTCAGCAATATCATATATCGTTTTGTTTTCGAAACTGAAAGGCATAATGGAATTTCTGAGCTGTTGGAGATTTTTGGAAGTGTAATTAGTGGGTTTGCTTTGCCACTGAAGGAGGAACACAAGATATTTTTGTGGAGGGCTCTGATTCCTCTACACAAACCAAAGTCCGTGGGTATTTATCATCAGCAGTTGACATATTGTGTTGTACAGTTTATAGATAAGGATCCGAAATTGGCGAGTACTGTAATAAAGGGGTTGTTGAAGTACTGGCCTGTAACAAATAGCCAGAAGGAGTTGATGTTTTTAAGCGAGTTAGAAGAGGTTTTGGAAATGACTAGCATGGATGAGTTTCAAAGGGTCATGGTTCCCCTGTTCCGGCGGATAAGATGCTGCCTCAATAGTTTTCATTACCAGGTGGCTGAACGAGCCCACTTGCTGTGGAACAATGAACACATCCTTAACCTTGTCGCACATAACCGCCAGGTGATCTTGCCGCTTGTCTTCCCAGCTATTGAGCGAAACACCCAACACCACTGGAATCAAGCAGTGCTGAACCTGACCCTGAATGTGAAGAAGACATTTTGCGATATGGATGAGGAGCTGGTAATTGCCTGCCAATGTAAGTTGGAGGAGGAAAATTTAAGGTCAAGCGCTGCAGCTGAGAAGCGAAGGTTAACATGGGAACGCTTGGAAACTGCGGCTGGGGTCCAACCTGTAGCCTGTAACTTTTTATCTCCCATAAAACCTCCTGCTTACCCTGTTGTCTGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03015 ko04071 ko04113 ko04114 ko04151 ko04152 ko04261 ko04728 ko05165 map03015 map04071 map04113 map04114 map04151 map04152 map04261 map04728 map05165
Pfam Domains
Protein Families
B56

Protein Analysis

503

Amino Acids

57.16

Weight (kDa)

8.11

Isoelectric Point (pI)

47.12

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 5 cut(s) 163, 1117, 1216, 1232, 1436
AclWI GGATC 5 cut(s) 308, 506, 615, 956, 969
AcsI RAATTY 2 cut(s) 789, 1375
AcuI CTGAAG 3 cut(s) 26, 156, 866
AfaI GTAC 5 cut(s) 118, 714, 945, 979, 1004
AfeI AGCGCT 1 cut(s) 1390
AfiI CCNNNNNNNGG 2 cut(s) 99, 1113
AgsI TTSAA 5 cut(s) 235, 246, 676, 1000, 1091
AjnI CCWGG 2 cut(s) 1149, 1218
AluBI AGCT 7 cut(s) 206, 280, 737, 799, 1247, 1339, 1397
AluI AGCT 7 cut(s) 206, 280, 737, 799, 1247, 1339, 1397
AlwI GGATC 5 cut(s) 308, 506, 615, 956, 969
Aor51HI AGCGCT 1 cut(s) 1390
AoxI GGCC 2 cut(s) 719, 1007
ApeKI GCWGC 5 cut(s) 81, 197, 1128, 1391, 1394
ApoI RAATTY 2 cut(s) 789, 1375
AspLEI GCGC 1 cut(s) 1391
AspS9I GGNCC 2 cut(s) 652, 1444
AsuHPI GGTGA 2 cut(s) 494, 1234
AsuII TTCGAA 1 cut(s) 768
AvaII GGWCC 2 cut(s) 652, 1444
BaeI ACNNNNGTAYC 2 cut(s) 443, 476
BamHI GGATCC 1 cut(s) 961
BanII GRGCYC 3 cut(s) 222, 880, 1168
BbsI GAAGAC 3 cut(s) 162, 1231, 1319
BbvCI CCTCAGC 1 cut(s) 207
BbvI GCAGC 5 cut(s) 93, 209, 1115, 1378, 1406
BccI CCATC 2 cut(s) 193, 231
BciT130I CCWGG 2 cut(s) 1151, 1220
BfaI CTAG 2 cut(s) 56, 1074
BfmI CTRYAG 3 cut(s) 63, 1392, 1452
BfoI RGCGCY 1 cut(s) 1392
BglII AGATCT 1 cut(s) 328
BisI GCNGC 7 cut(s) 82, 198, 1129, 1232, 1392, 1395, 1437
BlsI GCNGC 7 cut(s) 83, 199, 1130, 1233, 1393, 1396, 1438
BmcAI AGTACT 2 cut(s) 979, 1004
Bme1390I CCNGG 2 cut(s) 1151, 1220
Bme18I GGWCC 2 cut(s) 652, 1444
BmgT120I GGNCC 2 cut(s) 652, 1444
BmiI GGNNCC 5 cut(s) 219, 654, 963, 1104, 1445
BmrFI CCNGG 2 cut(s) 1151, 1220
BmrI ACTGGG 1 cut(s) 486
BmsI GCATC 2 cut(s) 201, 1115
BmuI ACTGGG 1 cut(s) 486
BpiI GAAGAC 3 cut(s) 162, 1231, 1319
Bpu10I CCTNAGC 2 cut(s) 17, 207
Bpu14I TTCGAA 1 cut(s) 768
Bsa29I ATCGAT 1 cut(s) 463
BsaBI GATNNNNATC 1 cut(s) 960
BsaJI CCNNGG 1 cut(s) 906
BsaXI ACNNNNNCTCC 2 cut(s) 1022, 1052
Bsc4I CCNNNNNNNGG 2 cut(s) 99, 1113
Bse1I ACTGG 4 cut(s) 317, 481, 1010, 1277
Bse8I GATNNNNATC 1 cut(s) 960
BseBI CCWGG 2 cut(s) 1151, 1220
BseCI ATCGAT 1 cut(s) 463
BseDI CCNNGG 1 cut(s) 906
BseGI GGATG 3 cut(s) 1087, 1191, 1336
BseJI GATNNNNATC 1 cut(s) 960
BseLI CCNNNNNNNGG 2 cut(s) 99, 1113
BseMII CTCAG 4 cut(s) 198, 639, 786, 1389
BseNI ACTGG 4 cut(s) 317, 481, 1010, 1277
BseRI GAGGAG 2 cut(s) 1349, 1382
BseXI GCAGC 5 cut(s) 93, 209, 1115, 1378, 1406
BseYI CCCAGC 2 cut(s) 1243, 1439
BsgI GTGCAG 2 cut(s) 393, 447
BshFI GGCC 2 cut(s) 721, 1009
BshVI ATCGAT 1 cut(s) 463
BsiSI CCGG 2 cut(s) 201, 1114
BslFI GGGAC 1 cut(s) 391
BslI CCNNNNNNNGG 2 cut(s) 99, 1113
BsmFI GGGAC 1 cut(s) 391
BsnI GGCC 2 cut(s) 721, 1009
Bsp119I TTCGAA 1 cut(s) 768
Bsp1286I GDGCHC 3 cut(s) 222, 880, 1168
Bsp1407I TGTACA 2 cut(s) 116, 943
Bsp143I GATC 8 cut(s) 313, 328, 464, 511, 544, 607, 961, 1224
BspACI CCGC 5 cut(s) 163, 1117, 1216, 1232, 1436
BspANI GGCC 2 cut(s) 721, 1009
BspCNI CTCAG 4 cut(s) 199, 640, 787, 1390
BspDI ATCGAT 1 cut(s) 463
BspLI GGNNCC 5 cut(s) 219, 654, 963, 1104, 1445
BspMAI CTGCAG 1 cut(s) 1396
BspPI GGATC 5 cut(s) 308, 506, 615, 956, 969
BspT104I TTCGAA 1 cut(s) 768
BsrGI TGTACA 2 cut(s) 116, 943
BsrI ACTGG 4 cut(s) 317, 481, 1010, 1277
BssECI CCNNGG 1 cut(s) 906
BssMI GATC 8 cut(s) 313, 328, 464, 511, 544, 607, 961, 1224
Bst2UI CCWGG 2 cut(s) 1151, 1220
Bst4CI ACNGT 4 cut(s) 382, 414, 948, 982
Bst6I CTCTTC 1 cut(s) 1050
BstAPI GCANNNNNTGC 1 cut(s) 407
BstAUI TGTACA 2 cut(s) 116, 943
BstBI TTCGAA 1 cut(s) 768
BstC8I GCNNGC 2 cut(s) 278, 1351
BstDEI CTNAG 6 cut(s) 17, 207, 456, 648, 795, 1398
BstDSI CCRYGG 1 cut(s) 906
BstF5I GGATG 3 cut(s) 1087, 1191, 1336
BstH2I RGCGCY 1 cut(s) 1392
BstHHI GCGC 1 cut(s) 1391
BstKTI GATC 8 cut(s) 316, 331, 467, 514, 547, 610, 964, 1227
BstMBI GATC 8 cut(s) 313, 328, 464, 511, 544, 607, 961, 1224
BstMWI GCNNNNNNNGC 3 cut(s) 203, 407, 1163
BstNI CCWGG 2 cut(s) 1151, 1220
BstNSI RCATGY 1 cut(s) 605
BstSCI CCNGG 2 cut(s) 1149, 1218
BstSFI CTRYAG 3 cut(s) 63, 1392, 1452
BstV1I GCAGC 5 cut(s) 93, 209, 1115, 1378, 1406
BstV2I GAAGAC 3 cut(s) 162, 1231, 1319
BstX2I RGATCY 2 cut(s) 328, 961
BstYI RGATCY 2 cut(s) 328, 961
Bsu15I ATCGAT 1 cut(s) 463
BsuRI GGCC 2 cut(s) 721, 1009
BsuTUI ATCGAT 1 cut(s) 463
BtgI CCRYGG 1 cut(s) 906
BtgZI GCGATG 1 cut(s) 178
BtsCI GGATG 3 cut(s) 1087, 1191, 1336
BtsI GCAGTG 2 cut(s) 219, 1290
BtsIMutI CAGTG 7 cut(s) 219, 344, 390, 410, 842, 1270, 1290
Cac8I GCNNGC 2 cut(s) 278, 1351
CfoI GCGC 1 cut(s) 1391
Cfr13I GGNCC 2 cut(s) 652, 1444
ClaI ATCGAT 1 cut(s) 463
CsiI ACCWGGT 1 cut(s) 1149
Csp6I GTAC 5 cut(s) 117, 713, 944, 978, 1003
CspCI CAANNNNNGTGG 2 cut(s) 888, 923
CviAII CATG 6 cut(s) 114, 602, 709, 1078, 1099, 1416
CviQI GTAC 5 cut(s) 117, 713, 944, 978, 1003
DdeI CTNAG 6 cut(s) 17, 207, 456, 648, 795, 1398
DpnI GATC 8 cut(s) 315, 330, 466, 513, 546, 609, 963, 1226
DpnII GATC 8 cut(s) 313, 328, 464, 511, 544, 607, 961, 1224
Eam1104I CTCTTC 1 cut(s) 1050
EarI CTCTTC 1 cut(s) 1050
EciI GGCGGA 1 cut(s) 1132
Eco24I GRGCYC 3 cut(s) 222, 880, 1168
Eco47I GGWCC 2 cut(s) 652, 1444
Eco47III AGCGCT 1 cut(s) 1390
Eco57I CTGAAG 3 cut(s) 26, 156, 866
EcoO109I RGGNCCY 1 cut(s) 652
EcoRII CCWGG 2 cut(s) 1149, 1218
EcoT38I GRGCYC 3 cut(s) 222, 880, 1168
FaeI CATG 6 cut(s) 117, 605, 712, 1081, 1102, 1419
FaqI GGGAC 1 cut(s) 391
FatI CATG 6 cut(s) 113, 601, 708, 1077, 1098, 1415
FauI CCCGC 1 cut(s) 156
Fnu4HI GCNGC 7 cut(s) 82, 198, 1129, 1232, 1392, 1395, 1437
FokI GGATG 3 cut(s) 1094, 1178, 1343
FriOI GRGCYC 3 cut(s) 222, 880, 1168
Fsp4HI GCNGC 7 cut(s) 82, 198, 1129, 1232, 1392, 1395, 1437
FspBI CTAG 2 cut(s) 56, 1074
GlaI GCGC 1 cut(s) 1390
GluI GCNGC 7 cut(s) 82, 198, 1129, 1232, 1392, 1395, 1437
GsaI CCCAGC 2 cut(s) 1247, 1443
HaeII RGCGCY 1 cut(s) 1392
HaeIII GGCC 2 cut(s) 721, 1009
HapII CCGG 2 cut(s) 201, 1114
HhaI GCGC 1 cut(s) 1391
Hin1II CATG 6 cut(s) 117, 605, 712, 1081, 1102, 1419
Hin6I GCGC 1 cut(s) 1389
HinP1I GCGC 1 cut(s) 1389
HincII GTYRAC 3 cut(s) 433, 930, 1413
HindII GTYRAC 3 cut(s) 433, 930, 1413
HinfI GANTC 7 cut(s) 50, 59, 74, 305, 644, 883, 1276
HpaI GTTAAC 1 cut(s) 1413
HpaII CCGG 2 cut(s) 201, 1114
HphI GGTGA 2 cut(s) 494, 1234
Hpy166II GTNNAC 6 cut(s) 390, 433, 575, 715, 930, 1413
Hpy188I TCNGA 6 cut(s) 49, 649, 732, 796, 882, 966
Hpy8I GTNNAC 6 cut(s) 390, 433, 575, 715, 930, 1413
Hpy99I CGWCG 1 cut(s) 305
HpyAV CCTTC 4 cut(s) 241, 841, 1021, 1401
HpyCH4III ACNGT 4 cut(s) 382, 414, 948, 982
HpyCH4IV ACGT 1 cut(s) 337
HpyCH4V TGCA 5 cut(s) 410, 428, 532, 688, 1394
HpyF10VI GCNNNNNNNGC 3 cut(s) 203, 407, 1163
HpyF3I CTNAG 6 cut(s) 17, 207, 456, 648, 795, 1398
HpySE526I ACGT 1 cut(s) 337
Hsp92II CATG 6 cut(s) 117, 605, 712, 1081, 1102, 1419
HspAI GCGC 1 cut(s) 1389
KspAI GTTAAC 1 cut(s) 1413
Kzo9I GATC 8 cut(s) 313, 328, 464, 511, 544, 607, 961, 1224
LmnI GCTCC 3 cut(s) 203, 217, 1336
Lsp1109I GCAGC 5 cut(s) 93, 209, 1115, 1378, 1406
LweI GCATC 2 cut(s) 201, 1115
MabI ACCWGGT 1 cut(s) 1149
MaeI CTAG 2 cut(s) 56, 1074
MaeII ACGT 1 cut(s) 337
MaeIII GTNAC 2 cut(s) 1012, 1460
MalI GATC 8 cut(s) 315, 330, 466, 513, 546, 609, 963, 1226
MboI GATC 8 cut(s) 313, 328, 464, 511, 544, 607, 961, 1224
MboII GAAGA 9 cut(s) 162, 363, 503, 509, 539, 1067, 1231, 1321, 1324
MfeI CAATTG 1 cut(s) 120
MflI RGATCY 2 cut(s) 328, 961
MhlI GDGCHC 3 cut(s) 222, 880, 1168
MlyI GAGTC 1 cut(s) 638
MmeI TCCRAC 3 cut(s) 783, 1344, 1471
MseI TTAA 6 cut(s) 149, 321, 1043, 1197, 1379, 1412
MspA1I CMGCKG 1 cut(s) 1397
MspI CCGG 2 cut(s) 201, 1114
MspR9I CCNGG 2 cut(s) 1151, 1220
MunI CAATTG 1 cut(s) 120
MvaI CCWGG 2 cut(s) 1151, 1220
MwoI GCNNNNNNNGC 3 cut(s) 203, 407, 1163
NdeII GATC 8 cut(s) 313, 328, 464, 511, 544, 607, 961, 1224
NlaIII CATG 6 cut(s) 117, 605, 712, 1081, 1102, 1419
NlaIV GGNNCC 5 cut(s) 219, 654, 963, 1104, 1445
NspI RCATGY 1 cut(s) 605
NspV TTCGAA 1 cut(s) 768
PcsI WCGNNNNNNNCGW 1 cut(s) 309
PfeI GAWTC 6 cut(s) 50, 59, 74, 305, 883, 1276
PkrI GCNGC 7 cut(s) 83, 199, 1130, 1233, 1393, 1396, 1438
PleI GAGTC 1 cut(s) 638
PpsI GAGTC 1 cut(s) 638
PpuMI RGGWCCY 1 cut(s) 652
Psp5II RGGWCCY 1 cut(s) 652
Psp6I CCWGG 2 cut(s) 1149, 1218
PspFI CCCAGC 2 cut(s) 1243, 1439
PspGI CCWGG 2 cut(s) 1149, 1218
PspN4I GGNNCC 5 cut(s) 219, 654, 963, 1104, 1445
PspPI GGNCC 2 cut(s) 652, 1444
PspPPI RGGWCCY 1 cut(s) 652
PstI CTGCAG 1 cut(s) 1396
PsuI RGATCY 2 cut(s) 328, 961
PvuII CAGCTG 1 cut(s) 1397
RsaI GTAC 5 cut(s) 118, 714, 945, 979, 1004
RsaNI GTAC 5 cut(s) 117, 713, 944, 978, 1003
SaqAI TTAA 6 cut(s) 149, 321, 1043, 1197, 1379, 1412
SatI GCNGC 7 cut(s) 82, 198, 1129, 1232, 1392, 1395, 1437
Sau3AI GATC 8 cut(s) 313, 328, 464, 511, 544, 607, 961, 1224
Sau96I GGNCC 2 cut(s) 652, 1444
ScaI AGTACT 2 cut(s) 979, 1004
SchI GAGTC 1 cut(s) 638
ScrFI CCNGG 2 cut(s) 1151, 1220
SduI GDGCHC 3 cut(s) 222, 880, 1168
SexAI ACCWGGT 1 cut(s) 1149
SfaNI GCATC 2 cut(s) 201, 1115
SfcI CTRYAG 3 cut(s) 63, 1392, 1452
SfuI TTCGAA 1 cut(s) 768
SinI GGWCC 2 cut(s) 652, 1444
SsiI CCGC 5 cut(s) 163, 1117, 1216, 1232, 1436
SspMI CTAG 2 cut(s) 56, 1074
StyD4I CCNGG 2 cut(s) 1149, 1218
TaaI ACNGT 4 cut(s) 382, 414, 948, 982
TaiI ACGT 1 cut(s) 340
TaqI TCGA 3 cut(s) 303, 463, 768
TatI WGTACW 4 cut(s) 116, 943, 977, 1002
TauI GCSGC 2 cut(s) 1234, 1439
TfiI GAWTC 6 cut(s) 50, 59, 74, 305, 883, 1276
Tru1I TTAA 6 cut(s) 149, 321, 1043, 1197, 1379, 1412
Tru9I TTAA 6 cut(s) 149, 321, 1043, 1197, 1379, 1412
TscAI CASTG 7 cut(s) 219, 351, 397, 417, 849, 1277, 1290
TseI GCWGC 5 cut(s) 81, 197, 1128, 1391, 1394
TspDTI ATGAA 4 cut(s) 510, 697, 1133, 1200
TspGWI ACGGA 1 cut(s) 895
TspRI CASTG 7 cut(s) 219, 351, 397, 417, 849, 1277, 1290
VpaK11BI GGWCC 2 cut(s) 652, 1444
XapI RAATTY 2 cut(s) 789, 1375
XceI RCATGY 1 cut(s) 605
XspI CTAG 2 cut(s) 56, 1074
ZrmI AGTACT 2 cut(s) 979, 1004
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.