Rh2AG048500

Aux IAA proteins are short-lived transcriptional factors that function as repressors of early auxin response genes at low auxin concentrations

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0001510
Physical Location & Seq
Reverse (-)
3815461 .. 3816437
977 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG048500.1

Sequence Viewer

Length: 690 bp
ATGACGATGAATTACAAGGACACTGAGCTGACCTTGGGATTACCCGGTGGTGGAAGCCGTGGTTGTTCAACAGGTGTAGTGGTCTTCGGAGAGTTGATGAAGAGCTCGATGAAACGTGGGTTCATGGATACCGATGATGATCTCAACAACCTTGACCTGGAAAGCTCTAATGGCGAGCCTCGTGGCCAAGCTAAATTGGATGATGGGCCGTCGATGGAAGCAGCTAAGTACTCTTCCAGCAGTACCAGTACTACTGCCGTGAAAGCCCCAACTGCAGAGGGTCAAGTAGTTGGGTGGCCGCCGGTGAGATCGACAAGAAATAAGGCCTTGGACTCGAAGAACAACAGCAGCAGTAAATATGTGAAAGTGGGTGCTGATGGAGCTCCTTACCTGCGGAAAGTAGATTTAGAGATGTATAAAAGTTACGAGGAGCTATTAACAGCCTTGGATAAAATTTTCACTTCCTTCACCATAAACGGTAGTACTATGGAGGATGATAGGAAGCAACAGATGCTTTTTATGGAGCATGCAGTAGTGAATGGAACAGTGGAATATAATCAATATGTGCTGACATATGAAGATAAGGATAATGACTGGATGTTAGTTGGAGACGTTCCTTGGAAAATGTTTATAGATTCGTGCAAGCGTATCCGGTTGATGAAAAGTTCAGAAGCTGCGGTCAGAAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0009719 GO:0009725 GO:0009733 GO:0010033 GO:0042221 GO:0050896
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
AUX_IAA

Protein Analysis

229

Amino Acids

25.37

Weight (kDa)

5.1

Isoelectric Point (pI)

33.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AUX_IAA PF02309 7 - 226 2.3e-58 AUX/IAA family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 399
AciI CCGC 3 cut(s) 299, 394, 677
AcoI YGGCCR 2 cut(s) 184, 296
AcsI RAATTY 1 cut(s) 453
AfaI GTAC 4 cut(s) 230, 244, 250, 484
AfiI CCNNNNNNNGG 2 cut(s) 50, 157
AgsI TTSAA 1 cut(s) 69
AjnI CCWGG 1 cut(s) 156
AluBI AGCT 8 cut(s) 28, 105, 165, 191, 224, 383, 433, 674
AluI AGCT 8 cut(s) 28, 105, 165, 191, 224, 383, 433, 674
Alw21I GWGCWC 2 cut(s) 107, 385
Alw26I GTCTC 1 cut(s) 603
AlwNI CAGNNNCTG 1 cut(s) 674
AoxI GGCC 4 cut(s) 184, 206, 296, 324
ApeKI GCWGC 3 cut(s) 221, 348, 674
ApoI RAATTY 1 cut(s) 453
AspS9I GGNCC 1 cut(s) 206
AsuC2I CCSGG 1 cut(s) 45
AsuHPI GGTGA 2 cut(s) 316, 460
BalI TGGCCA 1 cut(s) 186
BanII GRGCYC 2 cut(s) 107, 385
BauI CACGAG 1 cut(s) 180
BbsI GAAGAC 1 cut(s) 76
Bbv12I GWGCWC 2 cut(s) 107, 385
BbvI GCAGC 3 cut(s) 233, 360, 661
BccI CCATC 3 cut(s) 197, 208, 371
BceAI ACGGC 3 cut(s) 42, 193, 242
BciT130I CCWGG 1 cut(s) 158
BciVI GTATCC 2 cut(s) 121, 659
BcnI CCSGG 1 cut(s) 45
BcoDI GTCTC 1 cut(s) 603
BfmI CTRYAG 1 cut(s) 273
BfuAI ACCTGC 1 cut(s) 399
BfuI GTATCC 2 cut(s) 121, 659
BisI GCNGC 4 cut(s) 222, 299, 349, 675
BlsI GCNGC 4 cut(s) 223, 300, 350, 676
BmcAI AGTACT 3 cut(s) 230, 250, 484
Bme1390I CCNGG 2 cut(s) 45, 158
BmgT120I GGNCC 1 cut(s) 206
BmrFI CCNGG 2 cut(s) 45, 158
BmsI GCATC 1 cut(s) 501
BpiI GAAGAC 1 cut(s) 76
BpuMI CCSGG 1 cut(s) 45
BsaBI GATNNNNATC 1 cut(s) 138
BsaJI CCNNGG 5 cut(s) 33, 58, 327, 444, 617
BsaWI WCCGGW 1 cut(s) 651
Bsc4I CCNNNNNNNGG 2 cut(s) 50, 157
Bse118I RCCGGY 1 cut(s) 301
Bse1I ACTGG 2 cut(s) 246, 599
Bse8I GATNNNNATC 1 cut(s) 138
BseBI CCWGG 1 cut(s) 158
BseDI CCNNGG 5 cut(s) 33, 58, 327, 444, 617
BseGI GGATG 3 cut(s) 205, 499, 603
BseJI GATNNNNATC 1 cut(s) 138
BseLI CCNNNNNNNGG 2 cut(s) 50, 157
BseMII CTCAG 1 cut(s) 15
BseNI ACTGG 2 cut(s) 246, 599
BseRI GAGGAG 1 cut(s) 443
BseXI GCAGC 3 cut(s) 233, 360, 661
BshFI GGCC 4 cut(s) 186, 208, 298, 326
BsiHKAI GWGCWC 2 cut(s) 107, 385
BsiSI CCGG 3 cut(s) 45, 302, 652
BslI CCNNNNNNNGG 2 cut(s) 50, 157
BsmAI GTCTC 1 cut(s) 603
BsmBI CGTCTC 1 cut(s) 603
BsnI GGCC 4 cut(s) 186, 208, 298, 326
Bsp1286I GDGCHC 2 cut(s) 107, 385
Bsp143I GATC 2 cut(s) 139, 308
BspACI CCGC 3 cut(s) 299, 394, 677
BspANI GGCC 4 cut(s) 186, 208, 298, 326
BspCNI CTCAG 1 cut(s) 16
BspMAI CTGCAG 1 cut(s) 277
BspMI ACCTGC 1 cut(s) 399
BspQI GCTCTTC 1 cut(s) 95
BsrFI RCCGGY 1 cut(s) 301
BsrI ACTGG 2 cut(s) 246, 599
BssAI RCCGGY 1 cut(s) 301
BssECI CCNNGG 5 cut(s) 33, 58, 327, 444, 617
BssMI GATC 2 cut(s) 139, 308
BssSI CACGAG 1 cut(s) 180
BssT1I CCWWGG 4 cut(s) 33, 327, 444, 617
Bst2BI CACGAG 1 cut(s) 180
Bst2UI CCWGG 1 cut(s) 158
Bst4CI ACNGT 2 cut(s) 479, 547
Bst6I CTCTTC 2 cut(s) 95, 238
BstAPI GCANNNNNTGC 1 cut(s) 511
BstC8I GCNNGC 3 cut(s) 176, 528, 644
BstDEI CTNAG 2 cut(s) 24, 225
BstDSI CCRYGG 1 cut(s) 58
BstF5I GGATG 3 cut(s) 205, 499, 603
BstKTI GATC 2 cut(s) 142, 311
BstMAI GTCTC 1 cut(s) 603
BstMBI GATC 2 cut(s) 139, 308
BstMWI GCNNNNNNNGC 5 cut(s) 171, 263, 272, 380, 511
BstNI CCWGG 1 cut(s) 158
BstNSI RCATGY 1 cut(s) 530
BstSCI CCNGG 2 cut(s) 43, 156
BstSFI CTRYAG 1 cut(s) 273
BstV1I GCAGC 3 cut(s) 233, 360, 661
BstV2I GAAGAC 1 cut(s) 76
BsuI GTATCC 2 cut(s) 121, 659
BsuRI GGCC 4 cut(s) 186, 208, 298, 326
BtgI CCRYGG 1 cut(s) 58
BtsCI GGATG 3 cut(s) 205, 499, 603
BtsIMutI CAGTG 2 cut(s) 21, 552
BveI ACCTGC 1 cut(s) 399
Cac8I GCNNGC 3 cut(s) 176, 528, 644
CaiI CAGNNNCTG 1 cut(s) 674
Cfr10I RCCGGY 1 cut(s) 301
Cfr13I GGNCC 1 cut(s) 206
Csp6I GTAC 4 cut(s) 229, 243, 249, 483
CviAII CATG 2 cut(s) 124, 527
CviQI GTAC 4 cut(s) 229, 243, 249, 483
DdeI CTNAG 2 cut(s) 24, 225
DpnI GATC 2 cut(s) 141, 310
DpnII GATC 2 cut(s) 139, 308
EaeI YGGCCR 2 cut(s) 184, 296
Eam1104I CTCTTC 2 cut(s) 95, 238
EarI CTCTTC 2 cut(s) 95, 238
Ecl136II GAGCTC 2 cut(s) 105, 383
Eco130I CCWWGG 4 cut(s) 33, 327, 444, 617
Eco147I AGGCCT 1 cut(s) 326
Eco24I GRGCYC 2 cut(s) 107, 385
Eco53kI GAGCTC 2 cut(s) 105, 383
EcoICRI GAGCTC 2 cut(s) 105, 383
EcoRII CCWGG 1 cut(s) 156
EcoT14I CCWWGG 4 cut(s) 33, 327, 444, 617
EcoT38I GRGCYC 2 cut(s) 107, 385
ErhI CCWWGG 4 cut(s) 33, 327, 444, 617
Esp3I CGTCTC 1 cut(s) 603
FaeI CATG 2 cut(s) 127, 530
FatI CATG 2 cut(s) 123, 526
FauNDI CATATG 1 cut(s) 574
Fnu4HI GCNGC 4 cut(s) 222, 299, 349, 675
FokI GGATG 3 cut(s) 212, 506, 610
FriOI GRGCYC 2 cut(s) 107, 385
Fsp4HI GCNGC 4 cut(s) 222, 299, 349, 675
GluI GCNGC 4 cut(s) 222, 299, 349, 675
HaeIII GGCC 4 cut(s) 186, 208, 298, 326
HapII CCGG 3 cut(s) 45, 302, 652
Hin1II CATG 2 cut(s) 127, 530
HinfI GANTC 2 cut(s) 332, 635
HpaII CCGG 3 cut(s) 45, 302, 652
HphI GGTGA 2 cut(s) 316, 460
Hpy188I TCNGA 3 cut(s) 89, 670, 683
Hpy99I CGWCG 1 cut(s) 214
HpyAV CCTTC 1 cut(s) 475
HpyCH4III ACNGT 2 cut(s) 479, 547
HpyCH4IV ACGT 2 cut(s) 115, 612
HpyCH4V TGCA 3 cut(s) 275, 530, 642
HpyF10VI GCNNNNNNNGC 5 cut(s) 171, 263, 272, 380, 511
HpyF3I CTNAG 2 cut(s) 24, 225
HpySE526I ACGT 2 cut(s) 115, 612
Hsp92II CATG 2 cut(s) 127, 530
Kzo9I GATC 2 cut(s) 139, 308
LguI GCTCTTC 1 cut(s) 95
LmnI GCTCC 4 cut(s) 380, 388, 430, 523
Lsp1109I GCAGC 3 cut(s) 233, 360, 661
LweI GCATC 1 cut(s) 501
MaeII ACGT 2 cut(s) 115, 612
MaeIII GTNAC 1 cut(s) 422
MalI GATC 2 cut(s) 141, 310
MboI GATC 2 cut(s) 139, 308
MboII GAAGA 5 cut(s) 76, 112, 225, 349, 590
MhlI GDGCHC 2 cut(s) 107, 385
MlsI TGGCCA 1 cut(s) 186
MluCI AATT 4 cut(s) 10, 194, 453, 685
MluNI TGGCCA 1 cut(s) 186
MlyI GAGTC 1 cut(s) 326
MmeI TCCRAC 1 cut(s) 586
MnlI CCTC 4 cut(s) 189, 271, 421, 484
Mox20I TGGCCA 1 cut(s) 186
MscI TGGCCA 1 cut(s) 186
MseI TTAA 1 cut(s) 437
Msp20I TGGCCA 1 cut(s) 186
MspI CCGG 3 cut(s) 45, 302, 652
MspR9I CCNGG 2 cut(s) 45, 158
MvaI CCWGG 1 cut(s) 158
MwoI GCNNNNNNNGC 5 cut(s) 171, 263, 272, 380, 511
NciI CCSGG 1 cut(s) 45
NdeI CATATG 1 cut(s) 574
NdeII GATC 2 cut(s) 139, 308
NlaIII CATG 2 cut(s) 127, 530
NspI RCATGY 1 cut(s) 530
PaeI GCATGC 1 cut(s) 530
PceI AGGCCT 1 cut(s) 326
PciSI GCTCTTC 1 cut(s) 95
PfeI GAWTC 1 cut(s) 635
PkrI GCNGC 4 cut(s) 223, 300, 350, 676
PleI GAGTC 1 cut(s) 326
PpsI GAGTC 1 cut(s) 326
Psp124BI GAGCTC 2 cut(s) 107, 385
Psp6I CCWGG 1 cut(s) 156
PspGI CCWGG 1 cut(s) 156
PspPI GGNCC 1 cut(s) 206
PstI CTGCAG 1 cut(s) 277
PstNI CAGNNNCTG 1 cut(s) 674
RsaI GTAC 4 cut(s) 230, 244, 250, 484
RsaNI GTAC 4 cut(s) 229, 243, 249, 483
SacI GAGCTC 2 cut(s) 107, 385
SapI GCTCTTC 1 cut(s) 95
SaqAI TTAA 1 cut(s) 437
SatI GCNGC 4 cut(s) 222, 299, 349, 675
Sau3AI GATC 2 cut(s) 139, 308
Sau96I GGNCC 1 cut(s) 206
ScaI AGTACT 3 cut(s) 230, 250, 484
SchI GAGTC 1 cut(s) 326
ScrFI CCNGG 2 cut(s) 45, 158
SduI GDGCHC 2 cut(s) 107, 385
SfaNI GCATC 1 cut(s) 501
SfcI CTRYAG 1 cut(s) 273
SgrAI CRCCGGYG 1 cut(s) 301
SphI GCATGC 1 cut(s) 530
Sse9I AATT 4 cut(s) 10, 194, 453, 685
SseBI AGGCCT 1 cut(s) 326
SsiI CCGC 3 cut(s) 299, 394, 677
SstI GAGCTC 2 cut(s) 107, 385
StuI AGGCCT 1 cut(s) 326
StyD4I CCNGG 2 cut(s) 43, 156
StyI CCWWGG 4 cut(s) 33, 327, 444, 617
TaaI ACNGT 2 cut(s) 479, 547
TaiI ACGT 2 cut(s) 118, 615
TaqI TCGA 4 cut(s) 107, 212, 311, 335
TasI AATT 4 cut(s) 10, 194, 453, 685
TatI WGTACW 3 cut(s) 228, 248, 482
TauI GCSGC 1 cut(s) 301
TfiI GAWTC 1 cut(s) 635
Tru1I TTAA 1 cut(s) 437
Tru9I TTAA 1 cut(s) 437
TscAI CASTG 2 cut(s) 28, 552
TseI GCWGC 3 cut(s) 221, 348, 674
TspDTI ATGAA 6 cut(s) 23, 112, 113, 125, 591, 674
TspRI CASTG 2 cut(s) 28, 552
XapI RAATTY 1 cut(s) 453
XceI RCATGY 1 cut(s) 530
ZrmI AGTACT 3 cut(s) 230, 250, 484
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.