MD02G1061700.v1.1

The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0001510
Physical Location & Seq
Reverse (-)
4949664 .. 4952634
2971 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1061700.v1.1.491

Sequence Viewer

Length: 1644 bp
ATGTCTTTCTTTCCCTTTTGTTCCAAATTTTGTGGATACCAAGAGCTCCTCCTCTGCTCCTCTCATTTGCTAAAAAGGGTCTCTCTGTGTTGTTTTCACAAGCACACCCAGAAGCTCCAAAACAGGGTTTCCACCATGCTGAAGCAAATTCTAAGCAAACTTCCTCGCAAGTCGTCAAAATCTGATTCACTAGATTCTGCAGGGAGTGATTCTGGCAACAATACTCCCAATTTGGGGAGTGGGTTCCAATGTACAATTGGTGGGAGTTCTCTTACAAGCAAATTAAATGTTGTCAAGCGAGTGTCTTCAGCAGTTTTCCCGTCAAGCATTGCAGCTGGAGCTGAGGCAGTGGAGCCCCATCTATCCTTCAAGGAGGTTTCCAACCCTCAGAAGCATAGTCTATTTATCAGTAAGCTGAACCTTTGCTGTGAGTTTGTTGATTCCAGTGATCCAGTTAAGCAAGATCTCAAACGCGAAACATTGATAGAACTTGTTGATTATGTTTCTTCTGGGACTGTGAAGTTCACTGAACCAGCAATTTCTGCTGTGTGTAAAATGTGTGCAGTCAACCTGTTTAGAGTTTTTCCACCTAAGTACCGATCTAATTTTACTGGGGGTGAAACAGATGATGAAGAACCAATGTTTGATCCTTCTTGGTCATACCTGCAAATTGTGTATGATCTTCTGGTTCGGTTTATCAGTTATACTTCACTTGACATAAAGGTGGCAAAAAAGCATGTGGATCACTCTTTTATCGTAAAATTACTTGACCTCTTTGACTCTGAGGACCCAAGAGAAAGAGACTGTTTGAAAACCATTCTGCATAGGATTTATGGAAAGTTCATGGTACACAGACCCTTTATTCGGAAAGCTGTCAGCAATATCATATATCGTTTTGTTTTCGAAACTGAAAGGCATAATGGAATTTCTGAGCTGTTGGAAATTTTTGGAAGTGTAATTAGTGGGTTTGCTTTGCCATTGAAGGAGGAACACAAGATATTTTTGTGGAGGGCTCTTATTCCTCTACACAAACCAAAGTCAGTGGGTATTTATCATCAGCAATTGACATATTGTGTTGTACAGTTCATAGATAAGGATCCGAAGTTGGCAAGTACTGTAATAAAGGGTTTGTTGAAGTTCTGGCCTGTAACAAATAGCCAGAAGGAGTTGATGTTTCTAAGTGAGTTAGAAGAGGTTTTGGAAATGACTAGCATGGATGAGTTTCAAAGGATCATGGTTCCATTGTTCCGGCGCATAAGATGCTGCCTCAATAGTTCTCATTACCAGGTGGCTGAACGAGCCCACTTGCTGTGGAACAATGAACACATCCTTAACCTTATCGCACATAATCGCCAGGTAATCTTTCCTCTTGTCTTCCCAGCTATTGAGCGAAACACCCAAAACCACTGGAATCAAGCAGTGCTGAACCTGACCCTCAATGTGAAGAAGACATTTTGCGATATGGATGAGGAGCTGGTACTTGCCTGTCAATTTAAGTTGGAGGAGGAAAATATAAGGTCAAGCGCCGCAGCTGAGAAGCGAAGGTTAACATGGGAACGCTTGGAAACTGCTGCTGGTGTCCAACCTGTAGCCTGTAACTTTTTATCTCCCATAAAGCCTCCCGCGTGTCCTGTTGTCTGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03015 ko04071 ko04113 ko04114 ko04151 ko04152 ko04261 ko04728 ko05165 map03015 map04071 map04113 map04114 map04151 map04152 map04261 map04728 map05165
Pfam Domains
Protein Families
B56

Protein Analysis

548

Amino Acids

62.4

Weight (kDa)

8.58

Isoelectric Point (pI)

46.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B56 PF01603 122 - 525 1.5e-168 Protein phosphatase 2A regulatory B subunit (B56 family)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 672
AccII CGCG 2 cut(s) 474, 1625
AciI CCGC 2 cut(s) 1527, 1623
AclWI GGATC 6 cut(s) 443, 641, 750, 1091, 1104, 1238
AcsI RAATTY 4 cut(s) 26, 147, 924, 942
AcuI CTGAAG 2 cut(s) 161, 291
AfaI GTAC 6 cut(s) 253, 596, 849, 1080, 1114, 1479
AfiI CCNNNNNNNGG 4 cut(s) 124, 233, 234, 864
AgsI TTSAA 5 cut(s) 370, 811, 982, 1135, 1226
AjnI CCWGG 2 cut(s) 1284, 1353
Alw21I GWGCWC 1 cut(s) 48
Alw26I GTCTC 2 cut(s) 85, 795
AlwI GGATC 6 cut(s) 443, 641, 750, 1091, 1104, 1238
AoxI GGCC 1 cut(s) 1142
ApeKI GCWGC 4 cut(s) 332, 1263, 1529, 1571
ApoI RAATTY 4 cut(s) 26, 147, 924, 942
ArsI GACNNNNNNTTYG 2 cut(s) 846, 878
AspLEI GCGC 2 cut(s) 1254, 1526
AspS9I GGNCC 1 cut(s) 787
AsuHPI GGTGA 1 cut(s) 629
AsuII TTCGAA 1 cut(s) 903
AvaII GGWCC 1 cut(s) 787
BaeI ACNNNNGTAYC 2 cut(s) 578, 611
BamHI GGATCC 1 cut(s) 1096
BanII GRGCYC 4 cut(s) 48, 357, 1015, 1303
BbsI GAAGAC 3 cut(s) 297, 1366, 1454
Bbv12I GWGCWC 1 cut(s) 48
BbvCI CCTCAGC 1 cut(s) 342
BbvI GCAGC 4 cut(s) 344, 1250, 1541, 1558
BccI CCATC 1 cut(s) 366
BciT130I CCWGG 2 cut(s) 1286, 1355
BciVI GTATCC 1 cut(s) 29
BcoDI GTCTC 2 cut(s) 85, 795
BfaI CTAG 2 cut(s) 191, 1209
BfmI CTRYAG 2 cut(s) 198, 1587
BfoI RGCGCY 1 cut(s) 1527
BfuAI ACCTGC 1 cut(s) 672
BfuI GTATCC 1 cut(s) 29
BglII AGATCT 1 cut(s) 463
BisI GCNGC 5 cut(s) 333, 1264, 1527, 1530, 1572
BlsI GCNGC 5 cut(s) 334, 1265, 1528, 1531, 1573
BmcAI AGTACT 1 cut(s) 1114
Bme1390I CCNGG 2 cut(s) 1286, 1355
Bme18I GGWCC 1 cut(s) 787
BmgT120I GGNCC 1 cut(s) 787
BmiI GGNNCC 5 cut(s) 245, 354, 789, 1098, 1239
BmrFI CCNGG 2 cut(s) 1286, 1355
BmrI ACTGGG 1 cut(s) 621
BmsI GCATC 1 cut(s) 1250
BmuI ACTGGG 1 cut(s) 621
BpiI GAAGAC 3 cut(s) 297, 1366, 1454
BpmI CTGGAG 1 cut(s) 357
Bpu10I CCTNAGC 1 cut(s) 342
Bpu14I TTCGAA 1 cut(s) 903
BsaBI GATNNNNATC 1 cut(s) 1095
BsaI GGTCTC 1 cut(s) 85
BsaXI ACNNNNNCTCC 2 cut(s) 1157, 1187
Bsc4I CCNNNNNNNGG 4 cut(s) 124, 233, 234, 864
Bse1I ACTGG 4 cut(s) 444, 452, 616, 1412
Bse3DI GCAATG 1 cut(s) 327
Bse8I GATNNNNATC 1 cut(s) 1095
BseBI CCWGG 2 cut(s) 1286, 1355
BseGI GGATG 3 cut(s) 1222, 1326, 1471
BseJI GATNNNNATC 1 cut(s) 1095
BseLI CCNNNNNNNGG 4 cut(s) 124, 233, 234, 864
BseMI GCAATG 1 cut(s) 327
BseMII CTCAG 5 cut(s) 333, 401, 774, 921, 1524
BseNI ACTGG 4 cut(s) 444, 452, 616, 1412
BseRI GAGGAG 5 cut(s) 38, 41, 49, 1484, 1517
BseXI GCAGC 4 cut(s) 344, 1250, 1541, 1558
BseYI CCCAGC 1 cut(s) 1378
BsgI GTGCAG 1 cut(s) 582
Bsh1236I CGCG 2 cut(s) 474, 1625
BshFI GGCC 1 cut(s) 1144
BsiHKAI GWGCWC 1 cut(s) 48
BsiSI CCGG 1 cut(s) 1249
BslFI GGGAC 1 cut(s) 526
BslI CCNNNNNNNGG 4 cut(s) 124, 233, 234, 864
BsmAI GTCTC 2 cut(s) 85, 795
BsmFI GGGAC 1 cut(s) 526
BsnI GGCC 1 cut(s) 1144
Bso31I GGTCTC 1 cut(s) 85
Bsp119I TTCGAA 1 cut(s) 903
Bsp1286I GDGCHC 4 cut(s) 48, 357, 1015, 1303
Bsp1407I TGTACA 2 cut(s) 251, 1078
Bsp143I GATC 8 cut(s) 448, 463, 599, 646, 679, 742, 1096, 1230
BspACI CCGC 2 cut(s) 1527, 1623
BspANI GGCC 1 cut(s) 1144
BspCNI CTCAG 5 cut(s) 334, 400, 775, 922, 1525
BspFNI CGCG 2 cut(s) 474, 1625
BspLI GGNNCC 5 cut(s) 245, 354, 789, 1098, 1239
BspMAI CTGCAG 1 cut(s) 202
BspMI ACCTGC 1 cut(s) 672
BspPI GGATC 6 cut(s) 443, 641, 750, 1091, 1104, 1238
BspT104I TTCGAA 1 cut(s) 903
BspTNI GGTCTC 1 cut(s) 85
BsrDI GCAATG 1 cut(s) 327
BsrGI TGTACA 2 cut(s) 251, 1078
BsrI ACTGG 4 cut(s) 444, 452, 616, 1412
BssMI GATC 8 cut(s) 448, 463, 599, 646, 679, 742, 1096, 1230
Bst2UI CCWGG 2 cut(s) 1286, 1355
Bst4CI ACNGT 4 cut(s) 517, 806, 1083, 1117
Bst6I CTCTTC 1 cut(s) 1185
BstAPI GCANNNNNTGC 2 cut(s) 542, 1260
BstAUI TGTACA 2 cut(s) 251, 1078
BstBI TTCGAA 1 cut(s) 903
BstDEI CTNAG 8 cut(s) 152, 342, 387, 591, 783, 930, 1178, 1533
BstF5I GGATG 3 cut(s) 1222, 1326, 1471
BstFNI CGCG 2 cut(s) 474, 1625
BstH2I RGCGCY 1 cut(s) 1527
BstHHI GCGC 2 cut(s) 1254, 1526
BstKTI GATC 8 cut(s) 451, 466, 602, 649, 682, 745, 1099, 1233
BstMAI GTCTC 2 cut(s) 85, 795
BstMBI GATC 8 cut(s) 448, 463, 599, 646, 679, 742, 1096, 1230
BstMWI GCNNNNNNNGC 4 cut(s) 338, 542, 1260, 1298
BstNI CCWGG 2 cut(s) 1286, 1355
BstNSI RCATGY 1 cut(s) 740
BstSCI CCNGG 2 cut(s) 1284, 1353
BstSFI CTRYAG 2 cut(s) 198, 1587
BstUI CGCG 2 cut(s) 474, 1625
BstV1I GCAGC 4 cut(s) 344, 1250, 1541, 1558
BstV2I GAAGAC 3 cut(s) 297, 1366, 1454
BstX2I RGATCY 2 cut(s) 463, 1096
BstYI RGATCY 2 cut(s) 463, 1096
BsuI GTATCC 1 cut(s) 29
BsuRI GGCC 1 cut(s) 1144
BtsCI GGATG 3 cut(s) 1222, 1326, 1471
BtsI GCAGTG 2 cut(s) 354, 1425
BtsIMutI CAGTG 6 cut(s) 354, 451, 525, 1047, 1405, 1425
BveI ACCTGC 1 cut(s) 672
CfoI GCGC 2 cut(s) 1254, 1526
Cfr13I GGNCC 1 cut(s) 787
CsiI ACCWGGT 1 cut(s) 1284
Csp6I GTAC 6 cut(s) 252, 595, 848, 1079, 1113, 1478
CspCI CAANNNNNGTGG 4 cut(s) 13, 48, 1023, 1058
CviAII CATG 6 cut(s) 136, 737, 844, 1213, 1234, 1551
CviQI GTAC 6 cut(s) 252, 595, 848, 1079, 1113, 1478
DdeI CTNAG 8 cut(s) 152, 342, 387, 591, 783, 930, 1178, 1533
DpnI GATC 8 cut(s) 450, 465, 601, 648, 681, 744, 1098, 1232
DpnII GATC 8 cut(s) 448, 463, 599, 646, 679, 742, 1096, 1230
Eam1104I CTCTTC 1 cut(s) 1185
EarI CTCTTC 1 cut(s) 1185
Ecl136II GAGCTC 1 cut(s) 46
Eco24I GRGCYC 4 cut(s) 48, 357, 1015, 1303
Eco31I GGTCTC 1 cut(s) 85
Eco47I GGWCC 1 cut(s) 787
Eco53kI GAGCTC 1 cut(s) 46
Eco57I CTGAAG 2 cut(s) 161, 291
EcoICRI GAGCTC 1 cut(s) 46
EcoO109I RGGNCCY 1 cut(s) 787
EcoRII CCWGG 2 cut(s) 1284, 1353
EcoT38I GRGCYC 4 cut(s) 48, 357, 1015, 1303
FaeI CATG 6 cut(s) 139, 740, 847, 1216, 1237, 1554
FaqI GGGAC 1 cut(s) 526
FatI CATG 6 cut(s) 135, 736, 843, 1212, 1233, 1550
FauI CCCGC 1 cut(s) 1630
Fnu4HI GCNGC 5 cut(s) 333, 1264, 1527, 1530, 1572
FokI GGATG 3 cut(s) 1229, 1313, 1478
FriOI GRGCYC 4 cut(s) 48, 357, 1015, 1303
Fsp4HI GCNGC 5 cut(s) 333, 1264, 1527, 1530, 1572
FspBI CTAG 2 cut(s) 191, 1209
GlaI GCGC 2 cut(s) 1253, 1525
GluI GCNGC 5 cut(s) 333, 1264, 1527, 1530, 1572
GsaI CCCAGC 1 cut(s) 1382
GsuI CTGGAG 1 cut(s) 357
HaeII RGCGCY 1 cut(s) 1527
HaeIII GGCC 1 cut(s) 1144
HapII CCGG 1 cut(s) 1249
HhaI GCGC 2 cut(s) 1254, 1526
Hin1II CATG 6 cut(s) 139, 740, 847, 1216, 1237, 1554
Hin6I GCGC 2 cut(s) 1252, 1524
HinP1I GCGC 2 cut(s) 1252, 1524
HincII GTYRAC 2 cut(s) 568, 1548
HindII GTYRAC 2 cut(s) 568, 1548
HinfI GANTC 6 cut(s) 185, 194, 209, 440, 779, 1411
HpaI GTTAAC 1 cut(s) 1548
HpaII CCGG 1 cut(s) 1249
HphI GGTGA 1 cut(s) 629
Hpy166II GTNNAC 4 cut(s) 525, 568, 850, 1548
Hpy188I TCNGA 6 cut(s) 184, 390, 784, 867, 931, 1101
Hpy8I GTNNAC 4 cut(s) 525, 568, 850, 1548
HpyAV CCTTC 5 cut(s) 376, 660, 976, 1156, 1536
HpyCH4III ACNGT 4 cut(s) 517, 806, 1083, 1117
HpyCH4V TGCA 5 cut(s) 200, 332, 563, 667, 823
HpyF10VI GCNNNNNNNGC 4 cut(s) 338, 542, 1260, 1298
HpyF3I CTNAG 8 cut(s) 152, 342, 387, 591, 783, 930, 1178, 1533
Hsp92II CATG 6 cut(s) 139, 740, 847, 1216, 1237, 1554
HspAI GCGC 2 cut(s) 1252, 1524
KspAI GTTAAC 1 cut(s) 1548
Kzo9I GATC 8 cut(s) 448, 463, 599, 646, 679, 742, 1096, 1230
LmnI GCTCC 6 cut(s) 51, 62, 120, 338, 352, 1471
Lsp1109I GCAGC 4 cut(s) 344, 1250, 1541, 1558
LweI GCATC 1 cut(s) 1250
MabI ACCWGGT 1 cut(s) 1284
MaeI CTAG 2 cut(s) 191, 1209
MaeIII GTNAC 2 cut(s) 1147, 1595
MalI GATC 8 cut(s) 450, 465, 601, 648, 681, 744, 1098, 1232
MboI GATC 8 cut(s) 448, 463, 599, 646, 679, 742, 1096, 1230
MboII GAAGA 8 cut(s) 297, 498, 644, 674, 1202, 1366, 1456, 1459
MfeI CAATTG 2 cut(s) 255, 1061
MflI RGATCY 2 cut(s) 463, 1096
MhlI GDGCHC 4 cut(s) 48, 357, 1015, 1303
MlyI GAGTC 1 cut(s) 773
MmeI TCCRAC 4 cut(s) 405, 918, 1479, 1606
MseI TTAA 5 cut(s) 284, 456, 1332, 1494, 1547
MslI CAYNNNNRTG 1 cut(s) 722
MspA1I CMGCKG 2 cut(s) 335, 1532
MspI CCGG 1 cut(s) 1249
MspR9I CCNGG 2 cut(s) 1286, 1355
MunI CAATTG 2 cut(s) 255, 1061
MvaI CCWGG 2 cut(s) 1286, 1355
MvnI CGCG 2 cut(s) 474, 1625
MwoI GCNNNNNNNGC 4 cut(s) 338, 542, 1260, 1298
NdeII GATC 8 cut(s) 448, 463, 599, 646, 679, 742, 1096, 1230
NlaIII CATG 6 cut(s) 139, 740, 847, 1216, 1237, 1554
NlaIV GGNNCC 5 cut(s) 245, 354, 789, 1098, 1239
NspI RCATGY 1 cut(s) 740
NspV TTCGAA 1 cut(s) 903
PfeI GAWTC 5 cut(s) 185, 194, 209, 440, 1411
PkrI GCNGC 5 cut(s) 334, 1265, 1528, 1531, 1573
PleI GAGTC 1 cut(s) 773
PpsI GAGTC 1 cut(s) 773
PpuMI RGGWCCY 1 cut(s) 787
Psp124BI GAGCTC 1 cut(s) 48
Psp5II RGGWCCY 1 cut(s) 787
Psp6I CCWGG 2 cut(s) 1284, 1353
PspFI CCCAGC 1 cut(s) 1378
PspGI CCWGG 2 cut(s) 1284, 1353
PspN4I GGNNCC 5 cut(s) 245, 354, 789, 1098, 1239
PspPI GGNCC 1 cut(s) 787
PspPPI RGGWCCY 1 cut(s) 787
PstI CTGCAG 1 cut(s) 202
PsuI RGATCY 2 cut(s) 463, 1096
PvuII CAGCTG 2 cut(s) 335, 1532
RsaI GTAC 6 cut(s) 253, 596, 849, 1080, 1114, 1479
RsaNI GTAC 6 cut(s) 252, 595, 848, 1079, 1113, 1478
RseI CAYNNNNRTG 1 cut(s) 722
SacI GAGCTC 1 cut(s) 48
SaqAI TTAA 5 cut(s) 284, 456, 1332, 1494, 1547
SatI GCNGC 5 cut(s) 333, 1264, 1527, 1530, 1572
Sau3AI GATC 8 cut(s) 448, 463, 599, 646, 679, 742, 1096, 1230
Sau96I GGNCC 1 cut(s) 787
ScaI AGTACT 1 cut(s) 1114
SchI GAGTC 1 cut(s) 773
ScrFI CCNGG 2 cut(s) 1286, 1355
SduI GDGCHC 4 cut(s) 48, 357, 1015, 1303
SexAI ACCWGGT 1 cut(s) 1284
SfaNI GCATC 1 cut(s) 1250
SfcI CTRYAG 2 cut(s) 198, 1587
SfuI TTCGAA 1 cut(s) 903
SinI GGWCC 1 cut(s) 787
SmiMI CAYNNNNRTG 1 cut(s) 722
SsiI CCGC 2 cut(s) 1527, 1623
SspMI CTAG 2 cut(s) 191, 1209
SstI GAGCTC 1 cut(s) 48
StyD4I CCNGG 2 cut(s) 1284, 1353
TaaI ACNGT 4 cut(s) 517, 806, 1083, 1117
TaqI TCGA 1 cut(s) 903
TatI WGTACW 3 cut(s) 251, 1078, 1112
TauI GCSGC 1 cut(s) 1529
TfiI GAWTC 5 cut(s) 185, 194, 209, 440, 1411
Tru1I TTAA 5 cut(s) 284, 456, 1332, 1494, 1547
Tru9I TTAA 5 cut(s) 284, 456, 1332, 1494, 1547
TscAI CASTG 6 cut(s) 354, 451, 532, 1047, 1412, 1425
TseI GCWGC 4 cut(s) 332, 1263, 1529, 1571
TspDTI ATGAA 4 cut(s) 645, 832, 1075, 1335
TspRI CASTG 6 cut(s) 354, 451, 532, 1047, 1412, 1425
VpaK11BI GGWCC 1 cut(s) 787
XapI RAATTY 4 cut(s) 26, 147, 924, 942
XceI RCATGY 1 cut(s) 740
XcmI CCANNNNNNNNNTGG 1 cut(s) 254
XspI CTAG 2 cut(s) 191, 1209
ZrmI AGTACT 1 cut(s) 1114
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.