Rorug02G0003200

The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0001510
Physical Location & Seq
Forward (+)
330211 .. 333535
3325 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0003200.1

Sequence Viewer

Length: 1050 bp
ATGGCGGAGTTTGAAGTGAAGAACAAGCAAGTGTTGTTCAACAACTATGTCGAAGGCTTTCCCAAAGAGTCGGACATGATCGTTACCGATGCTTCCACCATCAGACTCAAAGTTCCGGCAGATGCTCCCAATTCCGTTTTGCTCAAGAACCTCTACTTGTCCTGCGATCCAGTCATGCGCATCCTCATGAGGAAACCTGGAGACGAAACTTCATCTTATTTTACGCCCTATGCCCCTGGCTCCCCTTTGAGTGGGTATGGAGTGGGTGAAGTTGTAGATTCTGGGCACCCAGACTTTAAGGAAGGTGACCTTGTCTGGGGAACAACCAAATGGGAACACTACACCTTAATCACGCAACCAGAAAGCCTCCATAAGATCCAACACGCTGACGTTCCTCTTTCCTACTATACCGGTATACTTGGTATGCCTGGGATGACCGCCTATGCAGGCTTCAATGAAGTTTGTTCTCCTAAGAAGGGAGAATATGTCTTCATTTCAGCGGCATCAGGTGCCGTTGGTCAGCTGGTTGGACAATTAGCAAAATTGTTGGGTTGTTATGTCGTTGGAAGTGCTGGAAGTAAAGAAAAGGTTGACCTTTTGAAGAATAAGTTAAATTTTGATGAAGCTTTCAACTATAAGGAAGAGCATGACTTAACCGCAGCTCTGAAAAGGTATTTTCCAGAAGGCATTGACATATACTTTGAGAATGTTGGTGGAAAATTTCTGGATGCAGTCCTGCTCAACATGAGAGTCCACGGCCGGATTGCAGTGTGTGGAATGATTTCACAATACCATCTTGAAAAGCCTGAAGGTGTGACAAATTTGATGTGCCTTGTATACAAGCGGATTCATATGCAAGGATTTACAGTCCGCGATTACTATCACCTCTATCCCAAGTACCTGGACTATATTCTGCCTTGCATAAGAGAGGGAAAGGTGATATACTTGGAAGACATTGCTAAAGGCCTTGAGAATGGACCATCAGCGTTGGTAGGACTCTTTAGTGGTCACAATGTTGGCAAACAACTAGTTTCAATTTCCCACCAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03015 ko04071 ko04113 ko04114 ko04151 ko04152 ko04261 ko04728 ko05165 map03015 map04071 map04113 map04114 map04151 map04152 map04261 map04728 map05165
Pfam Domains
Protein Families
B56

Protein Analysis

349

Amino Acids

38.8

Weight (kDa)

6.13

Isoelectric Point (pI)

24.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ADH_N_2 PF16884 9 - 125 2.7e-22 N-terminal domain of oxidoreductase
ADH_zinc_N PF00107 171 - 302 3.9e-23 Zinc-binding dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 179
AccB1I GGYRCC 2 cut(s) 285, 509
AccI GTMKAC 2 cut(s) 415, 837
AccII CGCG 1 cut(s) 873
AciI CCGC 6 cut(s) 5, 438, 500, 657, 844, 871
AclWI GGATC 2 cut(s) 161, 370
AcoI YGGCCR 1 cut(s) 757
AcsI RAATTY 3 cut(s) 613, 719, 820
AcuI CTGAAG 1 cut(s) 828
AfaI GTAC 1 cut(s) 899
AfiI CCNNNNNNNGG 3 cut(s) 251, 316, 476
AgeI ACCGGT 1 cut(s) 410
AgsI TTSAA 7 cut(s) 14, 40, 454, 601, 631, 800, 1035
AhlI ACTAGT 1 cut(s) 1027
AjnI CCWGG 4 cut(s) 196, 235, 427, 900
AluBI AGCT 3 cut(s) 523, 626, 662
AluI AGCT 3 cut(s) 523, 626, 662
Alw26I GTCTC 1 cut(s) 195
AlwI GGATC 2 cut(s) 161, 370
AoxI GGCC 2 cut(s) 757, 964
ApeKI GCWGC 1 cut(s) 659
ApoI RAATTY 3 cut(s) 613, 719, 820
ArsI GACNNNNNNTTYG 2 cut(s) 683, 715
AsiGI ACCGGT 1 cut(s) 410
Asp700I GAANNNNTTC 2 cut(s) 57, 781
AspLEI GCGC 1 cut(s) 180
AspS9I GGNCC 1 cut(s) 977
AsuHPI GGTGA 4 cut(s) 278, 317, 875, 949
AvaII GGWCC 1 cut(s) 977
BaeGI GKGCMC 1 cut(s) 288
BanI GGYRCC 2 cut(s) 285, 509
BbsI GAAGAC 2 cut(s) 481, 957
BbvI GCAGC 1 cut(s) 671
BccI CCATC 3 cut(s) 107, 801, 988
BceAI ACGGC 2 cut(s) 497, 772
BciT130I CCWGG 4 cut(s) 198, 237, 429, 902
BcoDI GTCTC 1 cut(s) 195
BcuI ACTAGT 1 cut(s) 1027
BfaI CTAG 1 cut(s) 1028
BisI GCNGC 2 cut(s) 501, 660
BlsI GCNGC 2 cut(s) 502, 661
Bme1390I CCNGG 4 cut(s) 198, 237, 429, 902
Bme18I GGWCC 1 cut(s) 977
BmgT120I GGNCC 1 cut(s) 977
BmiI GGNNCC 3 cut(s) 241, 287, 511
BmrFI CCNGG 4 cut(s) 198, 237, 429, 902
BmsI GCATC 5 cut(s) 79, 112, 189, 512, 718
BpiI GAAGAC 2 cut(s) 481, 957
BpmI CTGGAG 1 cut(s) 219
BpuEI CTTGAG 2 cut(s) 128, 989
BsaBI GATNNNNATC 1 cut(s) 879
BsaJI CCNNGG 3 cut(s) 235, 428, 754
BsaWI WCCGGW 1 cut(s) 410
BsaXI ACNNNNNCTCC 2 cut(s) 471, 501
Bsc4I CCNNNNNNNGG 3 cut(s) 251, 316, 476
Bse118I RCCGGY 1 cut(s) 410
Bse1I ACTGG 2 cut(s) 170, 1045
Bse3DI GCAATG 1 cut(s) 954
Bse8I GATNNNNATC 1 cut(s) 879
BseBI CCWGG 4 cut(s) 198, 237, 429, 902
BseDI CCNNGG 3 cut(s) 235, 428, 754
BseGI GGATG 3 cut(s) 180, 438, 733
BseJI GATNNNNATC 1 cut(s) 879
BseLI CCNNNNNNNGG 3 cut(s) 251, 316, 476
BseMI GCAATG 1 cut(s) 954
BseNI ACTGG 2 cut(s) 170, 1045
BseSI GKGCMC 1 cut(s) 288
BseX3I CGGCCG 1 cut(s) 757
BseXI GCAGC 1 cut(s) 671
Bsh1236I CGCG 1 cut(s) 873
Bsh1285I CGRYCG 1 cut(s) 760
BshFI GGCC 2 cut(s) 759, 966
BshNI GGYRCC 2 cut(s) 285, 509
BshTI ACCGGT 1 cut(s) 410
BsiEI CGRYCG 1 cut(s) 760
BsiSI CCGG 3 cut(s) 116, 411, 760
BslI CCNNNNNNNGG 3 cut(s) 251, 316, 476
BsmAI GTCTC 1 cut(s) 195
BsmBI CGTCTC 1 cut(s) 195
BsnI GGCC 2 cut(s) 759, 966
Bsp1286I GDGCHC 1 cut(s) 288
Bsp143I GATC 3 cut(s) 78, 166, 375
BspACI CCGC 6 cut(s) 5, 438, 500, 657, 844, 871
BspANI GGCC 2 cut(s) 759, 966
BspFNI CGCG 1 cut(s) 873
BspHI TCATGA 1 cut(s) 186
BspLI GGNNCC 3 cut(s) 241, 287, 511
BspPI GGATC 2 cut(s) 161, 370
BspQI GCTCTTC 1 cut(s) 636
BspT107I GGYRCC 2 cut(s) 285, 509
BsrDI GCAATG 1 cut(s) 954
BsrFI RCCGGY 1 cut(s) 410
BsrI ACTGG 2 cut(s) 170, 1045
BssAI RCCGGY 1 cut(s) 410
BssECI CCNNGG 3 cut(s) 235, 428, 754
BssMI GATC 3 cut(s) 78, 166, 375
BssNAI GTATAC 2 cut(s) 416, 838
Bst1107I GTATAC 2 cut(s) 416, 838
Bst2UI CCWGG 4 cut(s) 198, 237, 429, 902
Bst4CI ACNGT 1 cut(s) 868
Bst6I CTCTTC 1 cut(s) 636
BstAPI GCANNNNNTGC 1 cut(s) 509
BstC8I GCNNGC 1 cut(s) 448
BstDEI CTNAG 1 cut(s) 471
BstDSI CCRYGG 1 cut(s) 754
BstEII GGTNACC 1 cut(s) 305
BstF5I GGATG 3 cut(s) 180, 438, 733
BstFNI CGCG 1 cut(s) 873
BstHHI GCGC 1 cut(s) 180
BstKTI GATC 3 cut(s) 81, 169, 378
BstMAI GTCTC 1 cut(s) 195
BstMBI GATC 3 cut(s) 78, 166, 375
BstMCI CGRYCG 1 cut(s) 760
BstMWI GCNNNNNNNGC 1 cut(s) 509
BstNI CCWGG 4 cut(s) 198, 237, 429, 902
BstPI GGTNACC 1 cut(s) 305
BstSCI CCNGG 4 cut(s) 196, 235, 427, 900
BstSLI GKGCMC 1 cut(s) 288
BstUI CGCG 1 cut(s) 873
BstV1I GCAGC 1 cut(s) 671
BstV2I GAAGAC 2 cut(s) 481, 957
BstX2I RGATCY 1 cut(s) 375
BstXI CCANNNNNNTGG 1 cut(s) 901
BstYI RGATCY 1 cut(s) 375
BstZ17I GTATAC 2 cut(s) 416, 838
BstZI CGGCCG 1 cut(s) 757
BsuRI GGCC 2 cut(s) 759, 966
BtgI CCRYGG 1 cut(s) 754
BtsCI GGATG 3 cut(s) 180, 438, 733
BtsI GCAGTG 1 cut(s) 774
BtsIMutI CAGTG 1 cut(s) 774
Cac8I GCNNGC 1 cut(s) 448
CciI TCATGA 1 cut(s) 186
CfoI GCGC 1 cut(s) 180
Cfr10I RCCGGY 1 cut(s) 410
Cfr13I GGNCC 1 cut(s) 977
Csp6I GTAC 1 cut(s) 898
CspAI ACCGGT 1 cut(s) 410
CviAII CATG 5 cut(s) 76, 175, 187, 647, 745
CviQI GTAC 1 cut(s) 898
DdeI CTNAG 1 cut(s) 471
DpnI GATC 3 cut(s) 80, 168, 377
DpnII GATC 3 cut(s) 78, 166, 375
EaeI YGGCCR 1 cut(s) 757
EagI CGGCCG 1 cut(s) 757
Eam1104I CTCTTC 1 cut(s) 636
EarI CTCTTC 1 cut(s) 636
EciI GGCGGA 1 cut(s) 20
EclXI CGGCCG 1 cut(s) 757
Eco147I AGGCCT 1 cut(s) 966
Eco47I GGWCC 1 cut(s) 977
Eco52I CGGCCG 1 cut(s) 757
Eco57I CTGAAG 1 cut(s) 828
Eco91I GGTNACC 1 cut(s) 305
EcoO65I GGTNACC 1 cut(s) 305
EcoRII CCWGG 4 cut(s) 196, 235, 427, 900
Esp3I CGTCTC 1 cut(s) 195
FaeI CATG 5 cut(s) 79, 178, 190, 650, 748
FalI AAGNNNNNCTT 2 cut(s) 294, 326
FatI CATG 5 cut(s) 75, 174, 186, 646, 744
FauNDI CATATG 1 cut(s) 852
FblI GTMKAC 2 cut(s) 415, 837
Fnu4HI GCNGC 2 cut(s) 501, 660
FokI GGATG 3 cut(s) 167, 445, 740
Fsp4HI GCNGC 2 cut(s) 501, 660
FspAI RTGCGCAY 1 cut(s) 179
FspBI CTAG 1 cut(s) 1028
FspI TGCGCA 1 cut(s) 179
GlaI GCGC 1 cut(s) 179
GluI GCNGC 2 cut(s) 501, 660
GsuI CTGGAG 1 cut(s) 219
HaeIII GGCC 2 cut(s) 759, 966
HapII CCGG 3 cut(s) 116, 411, 760
HhaI GCGC 1 cut(s) 180
Hin1II CATG 5 cut(s) 79, 178, 190, 650, 748
Hin6I GCGC 1 cut(s) 178
HinP1I GCGC 1 cut(s) 178
HincII GTYRAC 1 cut(s) 592
HindII GTYRAC 1 cut(s) 592
HindIII AAGCTT 1 cut(s) 624
HinfI GANTC 6 cut(s) 68, 105, 278, 750, 847, 996
HpaII CCGG 3 cut(s) 116, 411, 760
HphI GGTGA 4 cut(s) 278, 317, 875, 949
Hpy166II GTNNAC 4 cut(s) 416, 592, 754, 838
Hpy188I TCNGA 3 cut(s) 73, 104, 666
Hpy188III TCNNGA 5 cut(s) 145, 187, 680, 725, 797
Hpy8I GTNNAC 4 cut(s) 416, 592, 754, 838
HpyAV CCTTC 5 cut(s) 47, 296, 469, 677, 803
HpyCH4III ACNGT 1 cut(s) 868
HpyCH4IV ACGT 1 cut(s) 390
HpyCH4V TGCA 5 cut(s) 446, 731, 767, 856, 921
HpyF10VI GCNNNNNNNGC 1 cut(s) 509
HpyF3I CTNAG 1 cut(s) 471
HpySE526I ACGT 1 cut(s) 390
Hsp92II CATG 5 cut(s) 79, 178, 190, 650, 748
HspAI GCGC 1 cut(s) 178
Kzo9I GATC 3 cut(s) 78, 166, 375
LguI GCTCTTC 1 cut(s) 636
LmnI GCTCC 2 cut(s) 130, 245
Lsp1109I GCAGC 1 cut(s) 671
LweI GCATC 5 cut(s) 79, 112, 189, 512, 718
MaeI CTAG 1 cut(s) 1028
MaeII ACGT 1 cut(s) 390
MaeIII GTNAC 4 cut(s) 82, 305, 814, 1007
MalI GATC 3 cut(s) 80, 168, 377
MboI GATC 3 cut(s) 78, 166, 375
MboII GAAGA 5 cut(s) 31, 481, 613, 653, 962
MflI RGATCY 1 cut(s) 375
MhlI GDGCHC 1 cut(s) 288
MluCI AATT 7 cut(s) 130, 533, 542, 613, 719, 820, 1035
MlyI GAGTC 4 cut(s) 77, 99, 759, 990
MmeI TCCRAC 4 cut(s) 51, 403, 508, 544
MnlI CCTC 7 cut(s) 161, 183, 194, 377, 405, 896, 922
MroXI GAANNNNTTC 2 cut(s) 57, 781
MseI TTAA 4 cut(s) 297, 347, 611, 653
MslI CAYNNNNRTG 1 cut(s) 185
MspA1I CMGCKG 2 cut(s) 500, 523
MspI CCGG 3 cut(s) 116, 411, 760
MspR9I CCNGG 4 cut(s) 198, 237, 429, 902
MvaI CCWGG 4 cut(s) 198, 237, 429, 902
MvnI CGCG 1 cut(s) 873
MwoI GCNNNNNNNGC 1 cut(s) 509
NdeI CATATG 1 cut(s) 852
NdeII GATC 3 cut(s) 78, 166, 375
NlaIII CATG 5 cut(s) 79, 178, 190, 650, 748
NlaIV GGNNCC 3 cut(s) 241, 287, 511
NmuCI GTSAC 3 cut(s) 305, 814, 1007
NsbI TGCGCA 1 cut(s) 179
PagI TCATGA 1 cut(s) 186
PceI AGGCCT 1 cut(s) 966
PciSI GCTCTTC 1 cut(s) 636
PdmI GAANNNNTTC 2 cut(s) 57, 781
PfeI GAWTC 2 cut(s) 278, 847
PflFI GACNNNGTC 1 cut(s) 311
PinAI ACCGGT 1 cut(s) 410
PkrI GCNGC 2 cut(s) 502, 661
PleI GAGTC 4 cut(s) 76, 99, 758, 990
PpsI GAGTC 4 cut(s) 76, 99, 758, 990
Psp6I CCWGG 4 cut(s) 196, 235, 427, 900
PspEI GGTNACC 1 cut(s) 305
PspGI CCWGG 4 cut(s) 196, 235, 427, 900
PspN4I GGNNCC 3 cut(s) 241, 287, 511
PspPI GGNCC 1 cut(s) 977
PsuI RGATCY 1 cut(s) 375
PsyI GACNNNGTC 1 cut(s) 311
PvuII CAGCTG 1 cut(s) 523
RsaI GTAC 1 cut(s) 899
RsaNI GTAC 1 cut(s) 898
RseI CAYNNNNRTG 1 cut(s) 185
SapI GCTCTTC 1 cut(s) 636
SaqAI TTAA 4 cut(s) 297, 347, 611, 653
SatI GCNGC 2 cut(s) 501, 660
Sau3AI GATC 3 cut(s) 78, 166, 375
Sau96I GGNCC 1 cut(s) 977
SchI GAGTC 4 cut(s) 77, 99, 759, 990
ScrFI CCNGG 4 cut(s) 198, 237, 429, 902
SduI GDGCHC 1 cut(s) 288
SfaNI GCATC 5 cut(s) 79, 112, 189, 512, 718
SinI GGWCC 1 cut(s) 977
SmiMI CAYNNNNRTG 1 cut(s) 185
SmlI CTYRAG 2 cut(s) 143, 968
SmoI CTYRAG 2 cut(s) 143, 968
SpeI ACTAGT 1 cut(s) 1027
Sse9I AATT 7 cut(s) 130, 533, 542, 613, 719, 820, 1035
SseBI AGGCCT 1 cut(s) 966
SsiI CCGC 6 cut(s) 5, 438, 500, 657, 844, 871
SspMI CTAG 1 cut(s) 1028
StuI AGGCCT 1 cut(s) 966
StyD4I CCNGG 4 cut(s) 196, 235, 427, 900
TaaI ACNGT 1 cut(s) 868
TaiI ACGT 1 cut(s) 393
TaqI TCGA 1 cut(s) 51
TasI AATT 7 cut(s) 130, 533, 542, 613, 719, 820, 1035
TauI GCSGC 1 cut(s) 503
TfiI GAWTC 2 cut(s) 278, 847
Tru1I TTAA 4 cut(s) 297, 347, 611, 653
Tru9I TTAA 4 cut(s) 297, 347, 611, 653
TscAI CASTG 1 cut(s) 774
TseFI GTSAC 3 cut(s) 305, 814, 1007
TseI GCWGC 1 cut(s) 659
Tsp45I GTSAC 3 cut(s) 305, 814, 1007
TspDTI ATGAA 5 cut(s) 201, 471, 481, 636, 839
TspGWI ACGGA 1 cut(s) 124
TspRI CASTG 1 cut(s) 774
Tth111I GACNNNGTC 1 cut(s) 311
VpaK11BI GGWCC 1 cut(s) 977
XapI RAATTY 3 cut(s) 613, 719, 820
XmiI GTMKAC 2 cut(s) 415, 837
XmnI GAANNNNTTC 2 cut(s) 57, 781
XspI CTAG 1 cut(s) 1028
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.