MD02G1042300.v1.1

The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0001510
Physical Location & Seq
Reverse (-)
3472999 .. 3476509
3511 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1042300.v1.1.491

Sequence Viewer

Length: 1545 bp
ATGTTTGTGTTCAGTTTAGCTAACTTGGGGTCCACCATGCTGAAGCAAATCCTAAGCAAACTTCCTCGCAAGTCGTCAAAGTGTGATTCACTAGATTCTATAGGGAGTGATTCTGGCAGCAATACTTCCAATTTGGGGAGTGGGTTCCCATGTACAATTGGTGGGAGTTCTCTTACAAGCAAATTAAATGTTGTCAAGCGGGTGTCTTCAGCAGTTTTCCCATCAAGCATTGCAGCTGGAGCTGAGGCAGTGGAGCCCCATCTATCCTTCAAGGAGGTTTCAAACCCACAGAAGCATAATCTATTTATCAGCAAGCTGAACCTTTGCTGTGAGTTCGTTGATTCCAGCGATCCAGTTAAGCAAGATCTCAAACGTGAAACATTAATAGAACTTGTTGATTATGTTTCTTCTGGGACTGTGAAGTTCACTGAACCAGCAATTTCTGCAGTGTGTAAAATGTGTGCAGTCAACCTATTTAGAGTTTTCCCACCTAAGTATCGATCTAATTTTACTGGCGGTGAAACAGAAGATGAAGAACCAATGTTTGATCCTGCTTGGTCATATCTGCAAATTGTGTATGATCTTCTGGTTCGGTTTATCAGTTATAGTTCACTTGATGTAAAGGTGGCAAAAAAGCATGTGGATCACTCTTTTATCGTAAAATTACTTGACCTCTTTGACTCTGAGGACCCAAGAGAAAGAGATTGTTTGAAAACCATTCTGCATAGGATTTATGGAAAGTTCATGGTACACAGACCATTTATCCGGAAAGCTGTAAGCAATATCATATATCGTTTTGTTTTCGAAACTGAAAGGCATAATGGAATTTCTGAGCTGTTGGAGATATTTGGAAGTGTAATTAGTGGGTTTGCTTTGCCACTGAAGGAGGAACACAAGATATTTTTGTGGAGGGCTCTGATTCCTCTACACAAACCAAAGTCCGTGGGTATTTATCATCAGCAGTTGACATATTGTGTTGTACAGTTTATAGATAAGGATCCGAAATTGGCGAGTACTGTAATAAAGGGCTTGTTGAAGTACTGGCCTGTAACAAATAGCCAGAAGGAGTTGATGTTTTTAAGCGAGTTAGAAGAGGTTTTGGAAATGACTAGCATGGACGAATTTCAAAGGATCATGGTTCCCCTGTTCCGGCGGATAAGATGCTGCCTTAATAGTTTTCATTACCAGGTGGCTGAACGAGCCCACTTGCTGTGGAACAATGAACACATCCTTAACCTTGTCGCACATAACCGCCAGGTGATCTTGCCGCTTGTCTTCCCAGCTATTGAGCGAAACACCCAACACCACTGGAATCAAGCGGTGCTGAACCTGACCCTGAATATGAAGAAGACATTTTGCGATATGGATGAGGAGCTGGTAATTGCCTGCCAATGTAAGTTGGAGGAGGAAAATCTAAGGTCAAGCGCTGCAGCTGAGAAGCGAAGGTTAACATGGGAACGCTTGGAAACTGCGGCCGGGGTCCAACCTGTAGCCTGTAACTTTTTATCTCCTATAAAACCTCCTGCTTACCATGTTGTCTGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03015 ko04071 ko04113 ko04114 ko04151 ko04152 ko04261 ko04728 ko05165 map03015 map04071 map04113 map04114 map04151 map04152 map04261 map04728 map05165
Pfam Domains
Protein Families
B56

Protein Analysis

515

Amino Acids

58.51

Weight (kDa)

8.09

Isoelectric Point (pI)

46.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B56 PF01603 89 - 492 1.6e-167 Protein phosphatase 2A regulatory B subunit (B56 family)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 765
AciI CCGC 7 cut(s) 199, 516, 1153, 1252, 1268, 1319, 1472
AclWI GGATC 6 cut(s) 344, 542, 651, 992, 1005, 1139
AcoI YGGCCR 1 cut(s) 1473
AcsI RAATTY 2 cut(s) 825, 1121
AcuI CTGAAG 3 cut(s) 62, 192, 902
AfaI GTAC 5 cut(s) 154, 750, 981, 1015, 1040
AfeI AGCGCT 1 cut(s) 1426
AfiI CCNNNNNNNGG 2 cut(s) 135, 1149
AgsI TTSAA 5 cut(s) 271, 282, 712, 1036, 1127
AjnI CCWGG 2 cut(s) 1185, 1254
AluBI AGCT 9 cut(s) 20, 236, 242, 316, 773, 835, 1283, 1375, 1433
AluI AGCT 9 cut(s) 20, 236, 242, 316, 773, 835, 1283, 1375, 1433
AlwI GGATC 6 cut(s) 344, 542, 651, 992, 1005, 1139
Aor13HI TCCGGA 1 cut(s) 765
Aor51HI AGCGCT 1 cut(s) 1426
AoxI GGCC 2 cut(s) 1043, 1473
ApeKI GCWGC 5 cut(s) 117, 233, 1164, 1427, 1430
ApoI RAATTY 2 cut(s) 825, 1121
AseI ATTAAT 1 cut(s) 383
AspLEI GCGC 1 cut(s) 1427
AspS9I GGNCC 3 cut(s) 30, 688, 1480
AsuC2I CCSGG 1 cut(s) 1477
AsuHPI GGTGA 2 cut(s) 530, 1270
AsuII TTCGAA 1 cut(s) 804
AvaII GGWCC 3 cut(s) 30, 688, 1480
BaeI ACNNNNGTAYC 2 cut(s) 479, 512
BamHI GGATCC 1 cut(s) 997
BanII GRGCYC 3 cut(s) 258, 916, 1204
BbsI GAAGAC 3 cut(s) 198, 1267, 1355
BbvCI CCTCAGC 1 cut(s) 243
BbvI GCAGC 5 cut(s) 129, 245, 1151, 1414, 1442
BccI CCATC 2 cut(s) 229, 267
BciT130I CCWGG 2 cut(s) 1187, 1256
BcnI CCSGG 1 cut(s) 1477
BfaI CTAG 2 cut(s) 92, 1110
BfmI CTRYAG 4 cut(s) 99, 444, 1428, 1488
BfoI RGCGCY 1 cut(s) 1428
BglII AGATCT 1 cut(s) 364
BisI GCNGC 7 cut(s) 118, 234, 1165, 1268, 1428, 1431, 1473
BlsI GCNGC 7 cut(s) 119, 235, 1166, 1269, 1429, 1432, 1474
BmcAI AGTACT 2 cut(s) 1015, 1040
Bme1390I CCNGG 3 cut(s) 1187, 1256, 1477
Bme18I GGWCC 3 cut(s) 30, 688, 1480
BmgT120I GGNCC 3 cut(s) 30, 688, 1480
BmiI GGNNCC 7 cut(s) 31, 146, 255, 690, 999, 1140, 1481
BmrFI CCNGG 3 cut(s) 1187, 1256, 1477
BmsI GCATC 1 cut(s) 1151
BpiI GAAGAC 3 cut(s) 198, 1267, 1355
BpmI CTGGAG 1 cut(s) 258
Bpu10I CCTNAGC 2 cut(s) 53, 243
Bpu14I TTCGAA 1 cut(s) 804
BpuMI CCSGG 1 cut(s) 1477
Bsa29I ATCGAT 1 cut(s) 499
BsaBI GATNNNNATC 1 cut(s) 996
BsaJI CCNNGG 2 cut(s) 942, 1476
BsaWI WCCGGW 1 cut(s) 765
BsaXI ACNNNNNCTCC 2 cut(s) 1058, 1088
Bsc4I CCNNNNNNNGG 2 cut(s) 135, 1149
Bse1I ACTGG 4 cut(s) 353, 517, 1046, 1313
Bse3DI GCAATG 1 cut(s) 228
Bse8I GATNNNNATC 1 cut(s) 996
BseAI TCCGGA 1 cut(s) 765
BseBI CCWGG 2 cut(s) 1187, 1256
BseCI ATCGAT 1 cut(s) 499
BseDI CCNNGG 2 cut(s) 942, 1476
BseGI GGATG 2 cut(s) 1227, 1372
BseJI GATNNNNATC 1 cut(s) 996
BseLI CCNNNNNNNGG 2 cut(s) 135, 1149
BseMI GCAATG 1 cut(s) 228
BseMII CTCAG 4 cut(s) 234, 675, 822, 1425
BseNI ACTGG 4 cut(s) 353, 517, 1046, 1313
BseRI GAGGAG 2 cut(s) 1385, 1418
BseX3I CGGCCG 1 cut(s) 1473
BseXI GCAGC 5 cut(s) 129, 245, 1151, 1414, 1442
BseYI CCCAGC 1 cut(s) 1279
BsgI GTGCAG 1 cut(s) 483
Bsh1285I CGRYCG 1 cut(s) 1476
BshFI GGCC 2 cut(s) 1045, 1475
BshVI ATCGAT 1 cut(s) 499
BsiEI CGRYCG 1 cut(s) 1476
BsiSI CCGG 3 cut(s) 766, 1150, 1476
BslFI GGGAC 1 cut(s) 427
BslI CCNNNNNNNGG 2 cut(s) 135, 1149
BsmFI GGGAC 1 cut(s) 427
BsnI GGCC 2 cut(s) 1045, 1475
Bsp119I TTCGAA 1 cut(s) 804
Bsp1286I GDGCHC 3 cut(s) 258, 916, 1204
Bsp13I TCCGGA 1 cut(s) 765
Bsp1407I TGTACA 2 cut(s) 152, 979
Bsp143I GATC 9 cut(s) 349, 364, 500, 547, 580, 643, 997, 1131, 1260
BspACI CCGC 7 cut(s) 199, 516, 1153, 1252, 1268, 1319, 1472
BspANI GGCC 2 cut(s) 1045, 1475
BspCNI CTCAG 4 cut(s) 235, 676, 823, 1426
BspDI ATCGAT 1 cut(s) 499
BspEI TCCGGA 1 cut(s) 765
BspLI GGNNCC 7 cut(s) 31, 146, 255, 690, 999, 1140, 1481
BspMAI CTGCAG 2 cut(s) 448, 1432
BspPI GGATC 6 cut(s) 344, 542, 651, 992, 1005, 1139
BspT104I TTCGAA 1 cut(s) 804
BsrDI GCAATG 1 cut(s) 228
BsrGI TGTACA 2 cut(s) 152, 979
BsrI ACTGG 4 cut(s) 353, 517, 1046, 1313
BssECI CCNNGG 2 cut(s) 942, 1476
BssMI GATC 9 cut(s) 349, 364, 500, 547, 580, 643, 997, 1131, 1260
Bst2UI CCWGG 2 cut(s) 1187, 1256
Bst4CI ACNGT 3 cut(s) 418, 984, 1018
Bst6I CTCTTC 1 cut(s) 1086
BstAPI GCANNNNNTGC 1 cut(s) 443
BstAUI TGTACA 2 cut(s) 152, 979
BstBI TTCGAA 1 cut(s) 804
BstC8I GCNNGC 2 cut(s) 314, 1387
BstDEI CTNAG 7 cut(s) 53, 243, 492, 684, 831, 1415, 1434
BstDSI CCRYGG 1 cut(s) 942
BstF5I GGATG 2 cut(s) 1227, 1372
BstH2I RGCGCY 1 cut(s) 1428
BstHHI GCGC 1 cut(s) 1427
BstKTI GATC 9 cut(s) 352, 367, 503, 550, 583, 646, 1000, 1134, 1263
BstMBI GATC 9 cut(s) 349, 364, 500, 547, 580, 643, 997, 1131, 1260
BstMCI CGRYCG 1 cut(s) 1476
BstMWI GCNNNNNNNGC 3 cut(s) 239, 443, 1199
BstNI CCWGG 2 cut(s) 1187, 1256
BstNSI RCATGY 1 cut(s) 641
BstSCI CCNGG 3 cut(s) 1185, 1254, 1475
BstSFI CTRYAG 4 cut(s) 99, 444, 1428, 1488
BstV1I GCAGC 5 cut(s) 129, 245, 1151, 1414, 1442
BstV2I GAAGAC 3 cut(s) 198, 1267, 1355
BstX2I RGATCY 2 cut(s) 364, 997
BstYI RGATCY 2 cut(s) 364, 997
BstZI CGGCCG 1 cut(s) 1473
Bsu15I ATCGAT 1 cut(s) 499
BsuRI GGCC 2 cut(s) 1045, 1475
BsuTUI ATCGAT 1 cut(s) 499
BtgI CCRYGG 1 cut(s) 942
BtsCI GGATG 2 cut(s) 1227, 1372
BtsI GCAGTG 2 cut(s) 255, 453
BtsIMutI CAGTG 5 cut(s) 255, 426, 453, 878, 1306
Cac8I GCNNGC 2 cut(s) 314, 1387
CfoI GCGC 1 cut(s) 1427
Cfr13I GGNCC 3 cut(s) 30, 688, 1480
ClaI ATCGAT 1 cut(s) 499
CsiI ACCWGGT 1 cut(s) 1185
Csp6I GTAC 5 cut(s) 153, 749, 980, 1014, 1039
CspCI CAANNNNNGTGG 2 cut(s) 924, 959
CviAII CATG 8 cut(s) 37, 150, 638, 745, 1114, 1135, 1452, 1532
CviQI GTAC 5 cut(s) 153, 749, 980, 1014, 1039
DdeI CTNAG 7 cut(s) 53, 243, 492, 684, 831, 1415, 1434
DpnI GATC 9 cut(s) 351, 366, 502, 549, 582, 645, 999, 1133, 1262
DpnII GATC 9 cut(s) 349, 364, 500, 547, 580, 643, 997, 1131, 1260
EaeI YGGCCR 1 cut(s) 1473
EagI CGGCCG 1 cut(s) 1473
Eam1104I CTCTTC 1 cut(s) 1086
EarI CTCTTC 1 cut(s) 1086
EciI GGCGGA 1 cut(s) 1168
EclXI CGGCCG 1 cut(s) 1473
Eco24I GRGCYC 3 cut(s) 258, 916, 1204
Eco47I GGWCC 3 cut(s) 30, 688, 1480
Eco47III AGCGCT 1 cut(s) 1426
Eco52I CGGCCG 1 cut(s) 1473
Eco57I CTGAAG 3 cut(s) 62, 192, 902
EcoO109I RGGNCCY 1 cut(s) 688
EcoRII CCWGG 2 cut(s) 1185, 1254
EcoT38I GRGCYC 3 cut(s) 258, 916, 1204
FaeI CATG 8 cut(s) 40, 153, 641, 748, 1117, 1138, 1455, 1535
FaqI GGGAC 1 cut(s) 427
FatI CATG 8 cut(s) 36, 149, 637, 744, 1113, 1134, 1451, 1531
FauI CCCGC 1 cut(s) 192
Fnu4HI GCNGC 7 cut(s) 118, 234, 1165, 1268, 1428, 1431, 1473
FokI GGATG 2 cut(s) 1214, 1379
FriOI GRGCYC 3 cut(s) 258, 916, 1204
Fsp4HI GCNGC 7 cut(s) 118, 234, 1165, 1268, 1428, 1431, 1473
FspBI CTAG 2 cut(s) 92, 1110
GlaI GCGC 1 cut(s) 1426
GluI GCNGC 7 cut(s) 118, 234, 1165, 1268, 1428, 1431, 1473
GsaI CCCAGC 1 cut(s) 1283
GsuI CTGGAG 1 cut(s) 258
HaeII RGCGCY 1 cut(s) 1428
HaeIII GGCC 2 cut(s) 1045, 1475
HapII CCGG 3 cut(s) 766, 1150, 1476
HhaI GCGC 1 cut(s) 1427
Hin1II CATG 8 cut(s) 40, 153, 641, 748, 1117, 1138, 1455, 1535
Hin6I GCGC 1 cut(s) 1425
HinP1I GCGC 1 cut(s) 1425
HincII GTYRAC 3 cut(s) 469, 966, 1449
HindII GTYRAC 3 cut(s) 469, 966, 1449
HinfI GANTC 7 cut(s) 86, 95, 110, 341, 680, 919, 1312
HpaI GTTAAC 1 cut(s) 1449
HpaII CCGG 3 cut(s) 766, 1150, 1476
HphI GGTGA 2 cut(s) 530, 1270
Hpy166II GTNNAC 7 cut(s) 33, 426, 469, 611, 751, 966, 1449
Hpy188I TCNGA 4 cut(s) 685, 832, 918, 1002
Hpy188III TCNNGA 1 cut(s) 766
Hpy8I GTNNAC 7 cut(s) 33, 426, 469, 611, 751, 966, 1449
HpyAV CCTTC 4 cut(s) 277, 877, 1057, 1437
HpyCH4III ACNGT 3 cut(s) 418, 984, 1018
HpyCH4IV ACGT 1 cut(s) 373
HpyCH4V TGCA 6 cut(s) 233, 446, 464, 568, 724, 1430
HpyF10VI GCNNNNNNNGC 3 cut(s) 239, 443, 1199
HpyF3I CTNAG 7 cut(s) 53, 243, 492, 684, 831, 1415, 1434
HpySE526I ACGT 1 cut(s) 373
Hsp92II CATG 8 cut(s) 40, 153, 641, 748, 1117, 1138, 1455, 1535
HspAI GCGC 1 cut(s) 1425
Kpn2I TCCGGA 1 cut(s) 765
KspAI GTTAAC 1 cut(s) 1449
Kzo9I GATC 9 cut(s) 349, 364, 500, 547, 580, 643, 997, 1131, 1260
LmnI GCTCC 3 cut(s) 239, 253, 1372
Lsp1109I GCAGC 5 cut(s) 129, 245, 1151, 1414, 1442
LweI GCATC 1 cut(s) 1151
MabI ACCWGGT 1 cut(s) 1185
MaeI CTAG 2 cut(s) 92, 1110
MaeII ACGT 1 cut(s) 373
MaeIII GTNAC 2 cut(s) 1048, 1496
MalI GATC 9 cut(s) 351, 366, 502, 549, 582, 645, 999, 1133, 1262
MboI GATC 9 cut(s) 349, 364, 500, 547, 580, 643, 997, 1131, 1260
MboII GAAGA 9 cut(s) 198, 399, 539, 545, 575, 1103, 1267, 1357, 1360
MfeI CAATTG 1 cut(s) 156
MflI RGATCY 2 cut(s) 364, 997
MhlI GDGCHC 3 cut(s) 258, 916, 1204
MlyI GAGTC 1 cut(s) 674
MmeI TCCRAC 3 cut(s) 819, 1380, 1507
MroI TCCGGA 1 cut(s) 765
MseI TTAA 7 cut(s) 185, 357, 383, 1079, 1170, 1233, 1448
MspA1I CMGCKG 2 cut(s) 236, 1433
MspI CCGG 3 cut(s) 766, 1150, 1476
MspR9I CCNGG 3 cut(s) 1187, 1256, 1477
MunI CAATTG 1 cut(s) 156
MvaI CCWGG 2 cut(s) 1187, 1256
MwoI GCNNNNNNNGC 3 cut(s) 239, 443, 1199
NciI CCSGG 1 cut(s) 1477
NdeII GATC 9 cut(s) 349, 364, 500, 547, 580, 643, 997, 1131, 1260
NlaIII CATG 8 cut(s) 40, 153, 641, 748, 1117, 1138, 1455, 1535
NlaIV GGNNCC 7 cut(s) 31, 146, 255, 690, 999, 1140, 1481
NspI RCATGY 1 cut(s) 641
NspV TTCGAA 1 cut(s) 804
PfeI GAWTC 6 cut(s) 86, 95, 110, 341, 919, 1312
PkrI GCNGC 7 cut(s) 119, 235, 1166, 1269, 1429, 1432, 1474
PleI GAGTC 1 cut(s) 674
PpsI GAGTC 1 cut(s) 674
PpuMI RGGWCCY 1 cut(s) 688
PshBI ATTAAT 1 cut(s) 383
Psp5II RGGWCCY 1 cut(s) 688
Psp6I CCWGG 2 cut(s) 1185, 1254
PspFI CCCAGC 1 cut(s) 1279
PspGI CCWGG 2 cut(s) 1185, 1254
PspN4I GGNNCC 7 cut(s) 31, 146, 255, 690, 999, 1140, 1481
PspPI GGNCC 3 cut(s) 30, 688, 1480
PspPPI RGGWCCY 1 cut(s) 688
PstI CTGCAG 2 cut(s) 448, 1432
PsuI RGATCY 2 cut(s) 364, 997
PvuII CAGCTG 2 cut(s) 236, 1433
RsaI GTAC 5 cut(s) 154, 750, 981, 1015, 1040
RsaNI GTAC 5 cut(s) 153, 749, 980, 1014, 1039
SaqAI TTAA 7 cut(s) 185, 357, 383, 1079, 1170, 1233, 1448
SatI GCNGC 7 cut(s) 118, 234, 1165, 1268, 1428, 1431, 1473
Sau3AI GATC 9 cut(s) 349, 364, 500, 547, 580, 643, 997, 1131, 1260
Sau96I GGNCC 3 cut(s) 30, 688, 1480
ScaI AGTACT 2 cut(s) 1015, 1040
SchI GAGTC 1 cut(s) 674
ScrFI CCNGG 3 cut(s) 1187, 1256, 1477
SduI GDGCHC 3 cut(s) 258, 916, 1204
SexAI ACCWGGT 1 cut(s) 1185
SfaNI GCATC 1 cut(s) 1151
SfcI CTRYAG 4 cut(s) 99, 444, 1428, 1488
SfuI TTCGAA 1 cut(s) 804
SinI GGWCC 3 cut(s) 30, 688, 1480
SsiI CCGC 7 cut(s) 199, 516, 1153, 1252, 1268, 1319, 1472
SspMI CTAG 2 cut(s) 92, 1110
StyD4I CCNGG 3 cut(s) 1185, 1254, 1475
TaaI ACNGT 3 cut(s) 418, 984, 1018
TaiI ACGT 1 cut(s) 376
TaqI TCGA 2 cut(s) 499, 804
TatI WGTACW 4 cut(s) 152, 979, 1013, 1038
TauI GCSGC 2 cut(s) 1270, 1475
TfiI GAWTC 6 cut(s) 86, 95, 110, 341, 919, 1312
Tru1I TTAA 7 cut(s) 185, 357, 383, 1079, 1170, 1233, 1448
Tru9I TTAA 7 cut(s) 185, 357, 383, 1079, 1170, 1233, 1448
TscAI CASTG 5 cut(s) 255, 433, 453, 885, 1313
TseI GCWGC 5 cut(s) 117, 233, 1164, 1427, 1430
TspDTI ATGAA 5 cut(s) 546, 733, 1169, 1236, 1358
TspGWI ACGGA 1 cut(s) 931
TspRI CASTG 5 cut(s) 255, 433, 453, 885, 1313
VpaK11BI GGWCC 3 cut(s) 30, 688, 1480
VspI ATTAAT 1 cut(s) 383
XapI RAATTY 2 cut(s) 825, 1121
XceI RCATGY 1 cut(s) 641
XspI CTAG 2 cut(s) 92, 1110
ZrmI AGTACT 2 cut(s) 1015, 1040
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.