RchiOBHm_Chr2g0089801

Aux IAA proteins are short-lived transcriptional factors that function as repressors of early auxin response genes at low auxin concentrations

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0001510
Physical Location & Seq
Reverse (-)
3858295 .. 3863898
5604 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ46510

Sequence Viewer

Length: 963 bp
ATGAACTATGAAGATAAGGATGGCGACTGGATGTTAGTTGGAGAGGTTCCTTGGAAAATGCTTAAGGAATCATGCAAGCGTATCAGGAATACAAAGTTCATGCATTACCTTATGCTCTATTTATGTCTAAAATGTGCAGGTAGCAGAGTTGCGCGGGGGTTTGGATCTGATACATACGTACCAGTTAAGAACAAGGAACAAATCCTACGCCGTACTTACACGTACTGGGCTCGCCGGAAAGCTGACGTGAACTGGTGGAATATGACGATGAATTACAAGGACACTGAGCTGACCTTGGGATTACCTGGTGGTGGAAGCCGTGGTTGTTCAACAGGTGTAGTGGTCTTCGGAGAGTTGATGAAGAGCTCGATGAAACGTGGGTTCATGGATACCGATGATGATCTCAACAACCTTGACCTGGAAAGCTCTAATGGAGATCGCGAGCCTCGTGGCCAAGCTAAATTGGATGATGGGCCGTCGATGGAAGCAGCTAAGTACTCTTCCAGCAGTACCAGTACTACTGCCGTGAAAGCCCCAACTGCAAAGGGTCAAGTAGTTGGGTGGCCGCCGGTGAGATCGACGAGAAATAAGGCCTTGGACTCGAAGAACCTGAAGAACAACAGCAGCAGTAAATATGTGAAAGTCGGTGCTGATGGAGCTCCTTACCTGCGGAAAGTAGATTTAGAGATGTATAAAAGTTACGAGGAGCTATTAACAGCCTTGGAGAAAATTTTCACTTCCTTCACCATAAACGGTAAGTACATGGAGGATGATAGGAAGCAACAGATGCTTTTTATGGAGCATGCAGTAGTGAATGGAACAGTGGAATATAATCAATATGTGCTGACATATGAAGATAAGGATAATGACTGGATGTTAGTTGGAGACGTTCCTTGGAAAATGTTTAAAGATTCGTGCAAGCGTATACGGTTGATGAAAAGTTCAGAAGCTGCGGATGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0009719 GO:0009725 GO:0009733 GO:0010033 GO:0042221 GO:0050896
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
AUX_IAA

Protein Analysis

320

Amino Acids

36.55

Weight (kDa)

8.71

Isoelectric Point (pI)

40.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AUX_IAA PF02309 2 - 30 6.3e-12 AUX/IAA family
AUX_IAA PF02309 94 - 318 7.1e-59 AUX/IAA family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 128, 675
AccI GTMKAC 1 cut(s) 925
AccII CGCG 2 cut(s) 154, 441
AciI CCGC 4 cut(s) 154, 566, 670, 953
AclWI GGATC 1 cut(s) 172
AcoI YGGCCR 2 cut(s) 451, 563
AcsI RAATTY 1 cut(s) 729
AcuI CTGAAG 1 cut(s) 632
AfaI GTAC 7 cut(s) 180, 214, 224, 497, 511, 517, 761
AfiI CCNNNNNNNGG 2 cut(s) 311, 418
AflII CTTAAG 1 cut(s) 62
AflIII ACRYGT 1 cut(s) 219
AgsI TTSAA 1 cut(s) 330
AjiI CACGTC 1 cut(s) 247
AjnI CCWGG 2 cut(s) 304, 417
AluBI AGCT 9 cut(s) 242, 289, 366, 426, 458, 491, 659, 709, 950
AluI AGCT 9 cut(s) 242, 289, 366, 426, 458, 491, 659, 709, 950
Alw21I GWGCWC 2 cut(s) 368, 661
Alw26I GTCTC 1 cut(s) 879
AlwI GGATC 1 cut(s) 172
AlwNI CAGNNNCTG 1 cut(s) 950
AoxI GGCC 4 cut(s) 451, 473, 563, 591
ApeKI GCWGC 3 cut(s) 488, 624, 950
ApoI RAATTY 1 cut(s) 729
Asp700I GAANNNNTTC 1 cut(s) 731
AspLEI GCGC 1 cut(s) 154
AspS9I GGNCC 1 cut(s) 473
AsuHPI GGTGA 2 cut(s) 583, 736
BaeI ACNNNNGTAYC 4 cut(s) 162, 162, 195, 195
BalI TGGCCA 1 cut(s) 453
BanII GRGCYC 3 cut(s) 232, 368, 661
BauI CACGAG 1 cut(s) 447
BbsI GAAGAC 1 cut(s) 337
Bbv12I GWGCWC 2 cut(s) 368, 661
BbvI GCAGC 3 cut(s) 500, 636, 937
BccI CCATC 4 cut(s) 14, 464, 475, 647
BceAI ACGGC 4 cut(s) 195, 303, 460, 509
BciT130I CCWGG 2 cut(s) 306, 419
BciVI GTATCC 1 cut(s) 382
BcoDI GTCTC 1 cut(s) 879
BfrI CTTAAG 1 cut(s) 62
BfuAI ACCTGC 2 cut(s) 128, 675
BfuI GTATCC 1 cut(s) 382
BisI GCNGC 4 cut(s) 489, 566, 625, 951
BlsI GCNGC 4 cut(s) 490, 567, 626, 952
BmcAI AGTACT 2 cut(s) 497, 517
Bme1390I CCNGG 2 cut(s) 306, 419
BmgBI CACGTC 1 cut(s) 247
BmgT120I GGNCC 1 cut(s) 473
BmiI GGNNCC 1 cut(s) 48
BmrFI CCNGG 2 cut(s) 306, 419
BmrI ACTGGG 1 cut(s) 235
BmsI GCATC 1 cut(s) 777
BmuI ACTGGG 1 cut(s) 235
BpiI GAAGAC 1 cut(s) 337
BsaAI YACGTR 2 cut(s) 178, 222
BsaBI GATNNNNATC 1 cut(s) 399
BsaJI CCNNGG 6 cut(s) 50, 294, 319, 594, 720, 893
Bsc4I CCNNNNNNNGG 2 cut(s) 311, 418
Bse118I RCCGGY 1 cut(s) 568
Bse1I ACTGG 6 cut(s) 32, 182, 230, 257, 513, 875
Bse8I GATNNNNATC 1 cut(s) 399
BseBI CCWGG 2 cut(s) 306, 419
BseDI CCNNGG 6 cut(s) 50, 294, 319, 594, 720, 893
BseGI GGATG 6 cut(s) 25, 36, 472, 775, 879, 961
BseJI GATNNNNATC 1 cut(s) 399
BseLI CCNNNNNNNGG 2 cut(s) 311, 418
BseMII CTCAG 1 cut(s) 276
BseNI ACTGG 6 cut(s) 32, 182, 230, 257, 513, 875
BseRI GAGGAG 1 cut(s) 719
BseXI GCAGC 3 cut(s) 500, 636, 937
BsgI GTGCAG 1 cut(s) 156
Bsh1236I CGCG 2 cut(s) 154, 441
BshFI GGCC 4 cut(s) 453, 475, 565, 593
BsiHKAI GWGCWC 2 cut(s) 368, 661
BsiSI CCGG 2 cut(s) 235, 569
BslI CCNNNNNNNGG 2 cut(s) 311, 418
BsmAI GTCTC 1 cut(s) 879
BsmBI CGTCTC 1 cut(s) 879
BsnI GGCC 4 cut(s) 453, 475, 565, 593
Bsp1286I GDGCHC 3 cut(s) 232, 368, 661
Bsp143I GATC 4 cut(s) 164, 400, 436, 575
Bsp68I TCGCGA 1 cut(s) 441
BspACI CCGC 4 cut(s) 154, 566, 670, 953
BspANI GGCC 4 cut(s) 453, 475, 565, 593
BspCNI CTCAG 1 cut(s) 277
BspFNI CGCG 2 cut(s) 154, 441
BspLI GGNNCC 1 cut(s) 48
BspMI ACCTGC 2 cut(s) 128, 675
BspPI GGATC 1 cut(s) 172
BspQI GCTCTTC 1 cut(s) 356
BspTI CTTAAG 1 cut(s) 62
BsrFI RCCGGY 1 cut(s) 568
BsrI ACTGG 6 cut(s) 32, 182, 230, 257, 513, 875
BssAI RCCGGY 1 cut(s) 568
BssECI CCNNGG 6 cut(s) 50, 294, 319, 594, 720, 893
BssMI GATC 4 cut(s) 164, 400, 436, 575
BssNAI GTATAC 1 cut(s) 926
BssSI CACGAG 1 cut(s) 447
BssT1I CCWWGG 5 cut(s) 50, 294, 594, 720, 893
Bst1107I GTATAC 1 cut(s) 926
Bst2BI CACGAG 1 cut(s) 447
Bst2UI CCWGG 2 cut(s) 306, 419
Bst4CI ACNGT 3 cut(s) 755, 823, 930
Bst6I CTCTTC 2 cut(s) 356, 505
BstAFI CTTAAG 1 cut(s) 62
BstAPI GCANNNNNTGC 1 cut(s) 787
BstBAI YACGTR 2 cut(s) 178, 222
BstC8I GCNNGC 5 cut(s) 77, 232, 443, 804, 920
BstDEI CTNAG 2 cut(s) 285, 492
BstDSI CCRYGG 1 cut(s) 319
BstF5I GGATG 6 cut(s) 25, 36, 472, 775, 879, 961
BstFNI CGCG 2 cut(s) 154, 441
BstHHI GCGC 1 cut(s) 154
BstKTI GATC 4 cut(s) 167, 403, 439, 578
BstMAI GTCTC 1 cut(s) 879
BstMBI GATC 4 cut(s) 164, 400, 436, 575
BstMWI GCNNNNNNNGC 4 cut(s) 530, 539, 656, 787
BstNI CCWGG 2 cut(s) 306, 419
BstNSI RCATGY 1 cut(s) 806
BstSCI CCNGG 2 cut(s) 304, 417
BstSNI TACGTA 1 cut(s) 178
BstUI CGCG 2 cut(s) 154, 441
BstV1I GCAGC 3 cut(s) 500, 636, 937
BstV2I GAAGAC 1 cut(s) 337
BstX2I RGATCY 1 cut(s) 164
BstYI RGATCY 1 cut(s) 164
BstZ17I GTATAC 1 cut(s) 926
BsuI GTATCC 1 cut(s) 382
BsuRI GGCC 4 cut(s) 453, 475, 565, 593
BtgI CCRYGG 1 cut(s) 319
BtrI CACGTC 1 cut(s) 247
BtsCI GGATG 6 cut(s) 25, 36, 472, 775, 879, 961
BtsIMutI CAGTG 2 cut(s) 282, 828
BtuMI TCGCGA 1 cut(s) 441
BveI ACCTGC 2 cut(s) 128, 675
Cac8I GCNNGC 5 cut(s) 77, 232, 443, 804, 920
CaiI CAGNNNCTG 1 cut(s) 950
CfoI GCGC 1 cut(s) 154
Cfr10I RCCGGY 1 cut(s) 568
Cfr13I GGNCC 1 cut(s) 473
CsiI ACCWGGT 1 cut(s) 304
Csp6I GTAC 7 cut(s) 179, 213, 223, 496, 510, 516, 760
CviAII CATG 5 cut(s) 72, 100, 385, 763, 803
CviQI GTAC 7 cut(s) 179, 213, 223, 496, 510, 516, 760
DdeI CTNAG 2 cut(s) 285, 492
DpnI GATC 4 cut(s) 166, 402, 438, 577
DpnII GATC 4 cut(s) 164, 400, 436, 575
DraI TTTAAA 1 cut(s) 907
EaeI YGGCCR 2 cut(s) 451, 563
Eam1104I CTCTTC 2 cut(s) 356, 505
EarI CTCTTC 2 cut(s) 356, 505
Ecl136II GAGCTC 2 cut(s) 366, 659
Eco105I TACGTA 1 cut(s) 178
Eco130I CCWWGG 5 cut(s) 50, 294, 594, 720, 893
Eco147I AGGCCT 1 cut(s) 593
Eco24I GRGCYC 3 cut(s) 232, 368, 661
Eco53kI GAGCTC 2 cut(s) 366, 659
Eco57I CTGAAG 1 cut(s) 632
EcoICRI GAGCTC 2 cut(s) 366, 659
EcoRII CCWGG 2 cut(s) 304, 417
EcoT14I CCWWGG 5 cut(s) 50, 294, 594, 720, 893
EcoT22I ATGCAT 1 cut(s) 105
EcoT38I GRGCYC 3 cut(s) 232, 368, 661
ErhI CCWWGG 5 cut(s) 50, 294, 594, 720, 893
Esp3I CGTCTC 1 cut(s) 879
FaeI CATG 5 cut(s) 75, 103, 388, 766, 806
FatI CATG 5 cut(s) 71, 99, 384, 762, 802
FauI CCCGC 1 cut(s) 147
FauNDI CATATG 1 cut(s) 850
FblI GTMKAC 1 cut(s) 925
Fnu4HI GCNGC 4 cut(s) 489, 566, 625, 951
FokI GGATG 5 cut(s) 32, 43, 479, 782, 886
FriOI GRGCYC 3 cut(s) 232, 368, 661
Fsp4HI GCNGC 4 cut(s) 489, 566, 625, 951
GlaI GCGC 1 cut(s) 153
GluI GCNGC 4 cut(s) 489, 566, 625, 951
HaeIII GGCC 4 cut(s) 453, 475, 565, 593
HapII CCGG 2 cut(s) 235, 569
HhaI GCGC 1 cut(s) 154
Hin1II CATG 5 cut(s) 75, 103, 388, 766, 806
Hin6I GCGC 1 cut(s) 152
HinP1I GCGC 1 cut(s) 152
HinfI GANTC 3 cut(s) 68, 599, 911
HpaII CCGG 2 cut(s) 235, 569
HphI GGTGA 2 cut(s) 583, 736
Hpy166II GTNNAC 2 cut(s) 250, 926
Hpy188I TCNGA 3 cut(s) 169, 350, 946
Hpy188III TCNNGA 2 cut(s) 85, 440
Hpy8I GTNNAC 2 cut(s) 250, 926
Hpy99I CGWCG 2 cut(s) 481, 583
HpyAV CCTTC 1 cut(s) 751
HpyCH4III ACNGT 3 cut(s) 755, 823, 930
HpyCH4IV ACGT 5 cut(s) 177, 221, 246, 376, 888
HpyCH4V TGCA 6 cut(s) 75, 103, 137, 542, 806, 918
HpyF10VI GCNNNNNNNGC 4 cut(s) 530, 539, 656, 787
HpyF3I CTNAG 2 cut(s) 285, 492
HpySE526I ACGT 5 cut(s) 177, 221, 246, 376, 888
Hsp92II CATG 5 cut(s) 75, 103, 388, 766, 806
HspAI GCGC 1 cut(s) 152
Kzo9I GATC 4 cut(s) 164, 400, 436, 575
LguI GCTCTTC 1 cut(s) 356
LmnI GCTCC 4 cut(s) 656, 664, 706, 799
Lsp1109I GCAGC 3 cut(s) 500, 636, 937
LweI GCATC 1 cut(s) 777
MabI ACCWGGT 1 cut(s) 304
MaeII ACGT 5 cut(s) 177, 221, 246, 376, 888
MaeIII GTNAC 1 cut(s) 698
MalI GATC 4 cut(s) 166, 402, 438, 577
MboI GATC 4 cut(s) 164, 400, 436, 575
MboII GAAGA 7 cut(s) 23, 337, 373, 492, 616, 625, 866
MflI RGATCY 1 cut(s) 164
MhlI GDGCHC 3 cut(s) 232, 368, 661
MlsI TGGCCA 1 cut(s) 453
MluCI AATT 3 cut(s) 271, 461, 729
MluNI TGGCCA 1 cut(s) 453
MlyI GAGTC 1 cut(s) 593
MmeI TCCRAC 2 cut(s) 19, 862
MnlI CCTC 4 cut(s) 37, 456, 697, 760
Mox20I TGGCCA 1 cut(s) 453
Mph1103I ATGCAT 1 cut(s) 105
MroXI GAANNNNTTC 1 cut(s) 731
MscI TGGCCA 1 cut(s) 453
MseI TTAA 4 cut(s) 63, 186, 713, 906
Msp20I TGGCCA 1 cut(s) 453
MspCI CTTAAG 1 cut(s) 62
MspI CCGG 2 cut(s) 235, 569
MspR9I CCNGG 2 cut(s) 306, 419
MvaI CCWGG 2 cut(s) 306, 419
MvnI CGCG 2 cut(s) 154, 441
MwoI GCNNNNNNNGC 4 cut(s) 530, 539, 656, 787
NdeI CATATG 1 cut(s) 850
NdeII GATC 4 cut(s) 164, 400, 436, 575
NlaIII CATG 5 cut(s) 75, 103, 388, 766, 806
NlaIV GGNNCC 1 cut(s) 48
NruI TCGCGA 1 cut(s) 441
NsiI ATGCAT 1 cut(s) 105
NspI RCATGY 1 cut(s) 806
PaeI GCATGC 1 cut(s) 806
PceI AGGCCT 1 cut(s) 593
PciSI GCTCTTC 1 cut(s) 356
PcsI WCGNNNNNNNCGW 1 cut(s) 445
PdmI GAANNNNTTC 1 cut(s) 731
PfeI GAWTC 2 cut(s) 68, 911
PkrI GCNGC 4 cut(s) 490, 567, 626, 952
PleI GAGTC 1 cut(s) 593
PpsI GAGTC 1 cut(s) 593
Ppu21I YACGTR 2 cut(s) 178, 222
Psp124BI GAGCTC 2 cut(s) 368, 661
Psp6I CCWGG 2 cut(s) 304, 417
PspGI CCWGG 2 cut(s) 304, 417
PspN4I GGNNCC 1 cut(s) 48
PspPI GGNCC 1 cut(s) 473
PsrI GAACNNNNNNTAC 2 cut(s) 189, 221
PstNI CAGNNNCTG 1 cut(s) 950
PsuI RGATCY 1 cut(s) 164
RruI TCGCGA 1 cut(s) 441
RsaI GTAC 7 cut(s) 180, 214, 224, 497, 511, 517, 761
RsaNI GTAC 7 cut(s) 179, 213, 223, 496, 510, 516, 760
SacI GAGCTC 2 cut(s) 368, 661
SapI GCTCTTC 1 cut(s) 356
SaqAI TTAA 4 cut(s) 63, 186, 713, 906
SatI GCNGC 4 cut(s) 489, 566, 625, 951
Sau3AI GATC 4 cut(s) 164, 400, 436, 575
Sau96I GGNCC 1 cut(s) 473
ScaI AGTACT 2 cut(s) 497, 517
SchI GAGTC 1 cut(s) 593
ScrFI CCNGG 2 cut(s) 306, 419
SduI GDGCHC 3 cut(s) 232, 368, 661
SexAI ACCWGGT 1 cut(s) 304
SfaNI GCATC 1 cut(s) 777
SgrAI CRCCGGYG 1 cut(s) 568
SmlI CTYRAG 1 cut(s) 62
SmoI CTYRAG 1 cut(s) 62
SnaBI TACGTA 1 cut(s) 178
SphI GCATGC 1 cut(s) 806
Sse9I AATT 3 cut(s) 271, 461, 729
SseBI AGGCCT 1 cut(s) 593
SsiI CCGC 4 cut(s) 154, 566, 670, 953
SstI GAGCTC 2 cut(s) 368, 661
StuI AGGCCT 1 cut(s) 593
StyD4I CCNGG 2 cut(s) 304, 417
StyI CCWWGG 5 cut(s) 50, 294, 594, 720, 893
TaaI ACNGT 3 cut(s) 755, 823, 930
TaiI ACGT 5 cut(s) 180, 224, 249, 379, 891
TaqI TCGA 4 cut(s) 368, 479, 578, 602
TasI AATT 3 cut(s) 271, 461, 729
TatI WGTACW 3 cut(s) 495, 515, 759
TauI GCSGC 1 cut(s) 568
TfiI GAWTC 2 cut(s) 68, 911
Tru1I TTAA 4 cut(s) 63, 186, 713, 906
Tru9I TTAA 4 cut(s) 63, 186, 713, 906
TscAI CASTG 2 cut(s) 289, 828
TseI GCWGC 3 cut(s) 488, 624, 950
TspDTI ATGAA 9 cut(s) 17, 24, 88, 284, 373, 374, 386, 867, 950
TspRI CASTG 2 cut(s) 289, 828
Vha464I CTTAAG 1 cut(s) 62
XapI RAATTY 1 cut(s) 729
XceI RCATGY 1 cut(s) 806
XmiI GTMKAC 1 cut(s) 925
XmnI GAANNNNTTC 1 cut(s) 731
ZrmI AGTACT 2 cut(s) 497, 517
Zsp2I ATGCAT 1 cut(s) 105
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.