RLG00000016055

The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0001510
Physical Location & Seq
Reverse (-)
3520823 .. 3523871
3049 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000016055

Sequence Viewer

Length: 1632 bp
ATGCCACACCGTCTCTTCTCTTCAATCGAGGAGGACCTCTTATTGATCTTCCATGGAGGTCTCTCTCTGCTGTTTCTTCACTCTTACGTTCATACCCAGAAGTTCAAAATACAGGACTCCACCATGCTTAAGCAAATTCTGAGCAAATTCCCTCGCAAGCCAAAATCAGAGTCACTAGATTCCACCGGGAGTGATTCTGGCAACAATAGTCCCAATTTGGGCAGTGGTTTCCAATGTACAATTGGTGGGAGTTCTCTTACAAGCAAATTAAATGTTGTCAAACGAGTGTCTTCAGCTGTTTTCCCAGCAAGCATTTCAGCTGGAGCAGAGGTAGTGGAGCCTCATCTGTCTTTCAAAGAGGTTTCAAATCCACAGAAGCAGAATCTGTTTATCAGTAAGTTGAATGTTTGCTGCGAGATCATTGATTCTAGCGATCCAGCTAAGCAAGATCTTAAGCGTGAAACGTTGATTGAACTTGTTGATTTTGTTTCTTCTGGATCTGCAAAGTTTAATGAACCAGCAATTTCTGCGATGTGTAAAATGTGTGCAACCAACCTGTTTAGAGTTTTCCCACCTAAGTACCGATCTAATTTTACTGGTGGTGAAACCGAAGATGAAGAACCAATGTTTGATCCTGCTTGGTCACATCTGCAAATTGTGTATGATCTACTGGTTCGGTTTATCAGTTACAATTCGCTTGACATAAAGGTGGCGAAAAAGCATGTAGATCATTCTTTTATTGTACGATTACTTGACCTCTTTGATTCCGAGGACCCAAGAGAAAGAGACTGCTTGAAAACTATTCTGCATAGGATCTATGGAAAATTCATGGTGCATAGACCTTTTATACGAAAAGCTGTCAGCAATATCATCTATCGTTTTGTTTTTGAAACTGAAAGGCATAATGGTATGTCTGAGCTGTTGGAGATTTTTGGAAGTGTAATTAGCGGGTTTGCTTTGCCATTGAAGGAGGAGCACAAGATATTTTTGTGGAGGGCTCTGATTCCTCTACACAAACCAAAATCAGTGGGAATTTATCATCAGCAATTGACATATTGCGTTGTACAGTTTATAGAGAAGGACCCGAAGTTGGCTAGCACTGTTATAAAGGGATTGTTGAAGTACTGGCCAGTAACAAATAGCCAGAAGGAGTTGATGTTTCTAAGTGAGTTGGAAGAGGTTTTGGAAATGACTAGTATGGACGAGTTCCAAAAGATCATGGTCCCTTTGTTCCGAAGAATTAGATGCTGCCTCAATAGTTCCCATTATCAGGTGGCTGAGCGAGCTCACTTGCTATGGAACAATGAGCACATCCTTAACCTTATCGGGCATAACCGCCAGGTGATTTTGCCACTTGTCTTCCCAGCTATTGAGCGGAACACCCAAAACCACTGGAATCAAGCAGTGCTCAACCTGACAATGAATGTGAGGAAGATGTTTTGCGAGATGGATGAGGAGCTGGTACTTGCCTGCCAATGTAAGTTGGAGGAGGAACATATAAAGTCAAATTCTGCAGCCGAGAAGCGAAGGTTAACATGGGAACGCTTGGAAACTGCTGCTGGCGTCCAACCTGTTGTAGCTTGTAACTTTGTAGCTCCCATCAAGCCTCCTACTTGTCCTGTTGTCTGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03015 ko04071 ko04113 ko04114 ko04151 ko04152 ko04261 ko04728 ko05165 map03015 map04071 map04113 map04114 map04151 map04152 map04261 map04728 map05165
Pfam Domains
Protein Families
B56

Protein Analysis

544

Amino Acids

62.03

Weight (kDa)

7.87

Isoelectric Point (pI)

47.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B56 PF01603 117 - 520 3e-172 Protein phosphatase 2A regulatory B subunit (B56 family)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1106
AccBSI CCGCTC 1 cut(s) 1375
AciI CCGC 3 cut(s) 948, 1336, 1375
AclI AACGTT 1 cut(s) 464
AclWI GGATC 4 cut(s) 428, 505, 626, 821
AcoI YGGCCR 1 cut(s) 1127
AcsI RAATTY 5 cut(s) 135, 146, 824, 1032, 1507
AcuI CTGAAG 1 cut(s) 276
AcyI GRCGYC 1 cut(s) 1563
AfaI GTAC 6 cut(s) 238, 581, 744, 1065, 1124, 1464
AfiI CCNNNNNNNGG 3 cut(s) 218, 1090, 1270
AflII CTTAAG 2 cut(s) 128, 452
AhlI ACTAGT 1 cut(s) 1193
AjnI CCWGG 1 cut(s) 1338
Alw21I GWGCWC 4 cut(s) 978, 1288, 1311, 1410
Alw26I GTCTC 3 cut(s) 17, 65, 780
AlwI GGATC 4 cut(s) 428, 505, 626, 821
AlwNI CAGNNNCTG 1 cut(s) 385
AoxI GGCC 1 cut(s) 1127
ApeKI GCWGC 4 cut(s) 411, 1248, 1514, 1556
ApoI RAATTY 5 cut(s) 135, 146, 824, 1032, 1507
ArsI GACNNNNNNTTYG 2 cut(s) 155, 187
AspS9I GGNCC 4 cut(s) 34, 772, 1081, 1222
AsuC2I CCSGG 1 cut(s) 187
AsuHPI GGTGA 2 cut(s) 614, 1354
AsuNHI GCTAGC 1 cut(s) 1094
AvaII GGWCC 4 cut(s) 34, 772, 1081, 1222
BaeI ACNNNNGTAYC 2 cut(s) 563, 596
BalI TGGCCA 1 cut(s) 1129
BanII GRGCYC 2 cut(s) 1000, 1288
BbsI GAAGAC 2 cut(s) 282, 1351
Bbv12I GWGCWC 4 cut(s) 978, 1288, 1311, 1410
BbvI GCAGC 4 cut(s) 398, 1235, 1526, 1543
BccI CCATC 2 cut(s) 1441, 1607
BciT130I CCWGG 1 cut(s) 1340
BcnI CCSGG 1 cut(s) 187
BcoDI GTCTC 3 cut(s) 17, 65, 780
BcuI ACTAGT 1 cut(s) 1193
BfaI CTAG 4 cut(s) 176, 429, 1095, 1194
BfmI CTRYAG 1 cut(s) 1512
BfrI CTTAAG 2 cut(s) 128, 452
BglII AGATCT 1 cut(s) 448
BisI GCNGC 4 cut(s) 412, 1249, 1515, 1557
BlpI GCTNAGC 2 cut(s) 441, 1278
BlsI GCNGC 4 cut(s) 413, 1250, 1516, 1558
BmcAI AGTACT 1 cut(s) 1124
Bme1390I CCNGG 2 cut(s) 187, 1340
Bme18I GGWCC 4 cut(s) 34, 772, 1081, 1222
BmgT120I GGNCC 4 cut(s) 34, 772, 1081, 1222
BmiI GGNNCC 4 cut(s) 339, 774, 1083, 1224
BmrFI CCNGG 2 cut(s) 187, 1340
BmsI GCATC 1 cut(s) 1235
BmtI GCTAGC 1 cut(s) 1098
BpiI GAAGAC 2 cut(s) 282, 1351
BpmI CTGGAG 1 cut(s) 342
Bpu1102I GCTNAGC 2 cut(s) 441, 1278
BpuMI CCSGG 1 cut(s) 187
BsaHI GRCGYC 1 cut(s) 1563
BsaI GGTCTC 1 cut(s) 65
BsaJI CCNNGG 2 cut(s) 52, 768
BsaXI ACNNNNNCTCC 4 cut(s) 315, 345, 1142, 1172
Bsc4I CCNNNNNNNGG 3 cut(s) 218, 1090, 1270
Bse1I ACTGG 5 cut(s) 601, 675, 1130, 1130, 1397
BseBI CCWGG 1 cut(s) 1340
BseDI CCNNGG 2 cut(s) 52, 768
BseGI GGATG 2 cut(s) 1311, 1456
BseLI CCNNNNNNNGG 3 cut(s) 218, 1090, 1270
BseMII CTCAG 3 cut(s) 131, 906, 1269
BseNI ACTGG 5 cut(s) 601, 675, 1130, 1130, 1397
BseRI GAGGAG 4 cut(s) 44, 986, 1469, 1502
BseXI GCAGC 4 cut(s) 398, 1235, 1526, 1543
BseYI CCCAGC 2 cut(s) 304, 1363
BshFI GGCC 1 cut(s) 1129
BsiHKAI GWGCWC 4 cut(s) 978, 1288, 1311, 1410
BsiSI CCGG 1 cut(s) 186
BslFI GGGAC 2 cut(s) 195, 1208
BslI CCNNNNNNNGG 3 cut(s) 218, 1090, 1270
BsmAI GTCTC 3 cut(s) 17, 65, 780
BsmBI CGTCTC 1 cut(s) 17
BsmFI GGGAC 2 cut(s) 195, 1208
BsnI GGCC 1 cut(s) 1129
Bso31I GGTCTC 1 cut(s) 65
Bsp1286I GDGCHC 5 cut(s) 978, 1000, 1288, 1311, 1410
Bsp1407I TGTACA 2 cut(s) 236, 1063
Bsp1720I GCTNAGC 2 cut(s) 441, 1278
Bsp19I CCATGG 1 cut(s) 52
BspACI CCGC 3 cut(s) 948, 1336, 1375
BspANI GGCC 1 cut(s) 1129
BspCNI CTCAG 3 cut(s) 132, 907, 1270
BspLI GGNNCC 4 cut(s) 339, 774, 1083, 1224
BspMAI CTGCAG 1 cut(s) 1516
BspOI GCTAGC 1 cut(s) 1098
BspPI GGATC 4 cut(s) 428, 505, 626, 821
BspTI CTTAAG 2 cut(s) 128, 452
BspTNI GGTCTC 1 cut(s) 65
BsrBI CCGCTC 1 cut(s) 1375
BsrGI TGTACA 2 cut(s) 236, 1063
BsrI ACTGG 5 cut(s) 601, 675, 1130, 1130, 1397
BssECI CCNNGG 2 cut(s) 52, 768
BssNI GRCGYC 1 cut(s) 1563
BssT1I CCWWGG 1 cut(s) 52
Bst2UI CCWGG 1 cut(s) 1340
Bst4CI ACNGT 3 cut(s) 11, 1068, 1102
Bst6I CTCTTC 3 cut(s) 20, 25, 1170
BstACI GRCGYC 1 cut(s) 1563
BstAFI CTTAAG 2 cut(s) 128, 452
BstAPI GCANNNNNTGC 1 cut(s) 527
BstAUI TGTACA 2 cut(s) 236, 1063
BstC8I GCNNGC 6 cut(s) 158, 310, 1096, 1284, 1471, 1561
BstDEI CTNAG 6 cut(s) 140, 441, 576, 915, 1163, 1278
BstDSI CCRYGG 1 cut(s) 52
BstF5I GGATG 2 cut(s) 1311, 1456
BstMAI GTCTC 3 cut(s) 17, 65, 780
BstMWI GCNNNNNNNGC 2 cut(s) 527, 1283
BstNI CCWGG 1 cut(s) 1340
BstNSI RCATGY 1 cut(s) 725
BstSCI CCNGG 2 cut(s) 185, 1338
BstSFI CTRYAG 1 cut(s) 1512
BstV1I GCAGC 4 cut(s) 398, 1235, 1526, 1543
BstV2I GAAGAC 2 cut(s) 282, 1351
BstX2I RGATCY 3 cut(s) 448, 497, 813
BstYI RGATCY 3 cut(s) 448, 497, 813
BsuRI GGCC 1 cut(s) 1129
BtgI CCRYGG 1 cut(s) 52
BtgZI GCGATG 1 cut(s) 545
BtsCI GGATG 2 cut(s) 1311, 1456
BtsI GCAGTG 2 cut(s) 229, 1410
BtsIMutI CAGTG 5 cut(s) 229, 1032, 1098, 1390, 1410
Cac8I GCNNGC 6 cut(s) 158, 310, 1096, 1284, 1471, 1561
CaiI CAGNNNCTG 1 cut(s) 385
Cfr13I GGNCC 4 cut(s) 34, 772, 1081, 1222
CseI GACGC 1 cut(s) 1552
Csp6I GTAC 6 cut(s) 237, 580, 743, 1064, 1123, 1463
CspCI CAANNNNNGTGG 2 cut(s) 1008, 1043
CviAII CATG 6 cut(s) 53, 124, 722, 829, 1219, 1536
CviQI GTAC 6 cut(s) 237, 580, 743, 1064, 1123, 1463
DdeI CTNAG 6 cut(s) 140, 441, 576, 915, 1163, 1278
EaeI YGGCCR 1 cut(s) 1127
Eam1104I CTCTTC 3 cut(s) 20, 25, 1170
EarI CTCTTC 3 cut(s) 20, 25, 1170
Ecl136II GAGCTC 1 cut(s) 1286
Eco130I CCWWGG 1 cut(s) 52
Eco24I GRGCYC 2 cut(s) 1000, 1288
Eco31I GGTCTC 1 cut(s) 65
Eco47I GGWCC 4 cut(s) 34, 772, 1081, 1222
Eco53kI GAGCTC 1 cut(s) 1286
Eco57I CTGAAG 1 cut(s) 276
EcoICRI GAGCTC 1 cut(s) 1286
EcoO109I RGGNCCY 3 cut(s) 34, 772, 1081
EcoRII CCWGG 1 cut(s) 1338
EcoT14I CCWWGG 1 cut(s) 52
EcoT38I GRGCYC 2 cut(s) 1000, 1288
ErhI CCWWGG 1 cut(s) 52
Esp3I CGTCTC 1 cut(s) 17
FaeI CATG 6 cut(s) 56, 127, 725, 832, 1222, 1539
FaqI GGGAC 2 cut(s) 195, 1208
FatI CATG 6 cut(s) 52, 123, 721, 828, 1218, 1535
FauI CCCGC 1 cut(s) 941
Fnu4HI GCNGC 4 cut(s) 412, 1249, 1515, 1557
FokI GGATG 2 cut(s) 1298, 1463
FriOI GRGCYC 2 cut(s) 1000, 1288
Fsp4HI GCNGC 4 cut(s) 412, 1249, 1515, 1557
FspBI CTAG 4 cut(s) 176, 429, 1095, 1194
GluI GCNGC 4 cut(s) 412, 1249, 1515, 1557
GsaI CCCAGC 2 cut(s) 308, 1367
GsuI CTGGAG 1 cut(s) 342
HaeIII GGCC 1 cut(s) 1129
HapII CCGG 1 cut(s) 186
HgaI GACGC 1 cut(s) 1552
Hin1I GRCGYC 1 cut(s) 1563
Hin1II CATG 6 cut(s) 56, 127, 725, 832, 1222, 1539
HincII GTYRAC 1 cut(s) 1533
HindII GTYRAC 1 cut(s) 1533
HinfI GANTC 9 cut(s) 116, 170, 179, 194, 382, 425, 764, 1003, 1396
HpaI GTTAAC 1 cut(s) 1533
HpaII CCGG 1 cut(s) 186
HphI GGTGA 2 cut(s) 614, 1354
Hpy166II GTNNAC 1 cut(s) 1533
Hpy188I TCNGA 6 cut(s) 141, 169, 769, 916, 1002, 1235
Hpy188III TCNNGA 1 cut(s) 495
Hpy8I GTNNAC 1 cut(s) 1533
HpyAV CCTTC 4 cut(s) 961, 1072, 1141, 1521
HpyCH4III ACNGT 3 cut(s) 11, 1068, 1102
HpyCH4IV ACGT 2 cut(s) 87, 464
HpyCH4V TGCA 6 cut(s) 503, 548, 652, 808, 835, 1514
HpyF10VI GCNNNNNNNGC 2 cut(s) 527, 1283
HpyF3I CTNAG 6 cut(s) 140, 441, 576, 915, 1163, 1278
HpySE526I ACGT 2 cut(s) 87, 464
Hsp92I GRCGYC 1 cut(s) 1563
Hsp92II CATG 6 cut(s) 56, 127, 725, 832, 1222, 1539
KspAI GTTAAC 1 cut(s) 1533
LmnI GCTCC 5 cut(s) 323, 337, 973, 1456, 1600
Lsp1109I GCAGC 4 cut(s) 398, 1235, 1526, 1543
LweI GCATC 1 cut(s) 1235
MaeI CTAG 4 cut(s) 176, 429, 1095, 1194
MaeII ACGT 2 cut(s) 87, 464
MaeIII GTNAC 5 cut(s) 171, 642, 686, 1132, 1583
MbiI CCGCTC 1 cut(s) 1375
MfeI CAATTG 2 cut(s) 240, 1046
MflI RGATCY 3 cut(s) 448, 497, 813
MhlI GDGCHC 5 cut(s) 978, 1000, 1288, 1311, 1410
MlsI TGGCCA 1 cut(s) 1129
MluNI TGGCCA 1 cut(s) 1129
MlyI GAGTC 2 cut(s) 110, 179
MmeI TCCRAC 4 cut(s) 903, 1152, 1464, 1591
Mox20I TGGCCA 1 cut(s) 1129
MscI TGGCCA 1 cut(s) 1129
MseI TTAA 6 cut(s) 129, 269, 453, 510, 1317, 1532
MslI CAYNNNNRTG 1 cut(s) 707
Msp20I TGGCCA 1 cut(s) 1129
MspA1I CMGCKG 2 cut(s) 296, 320
MspCI CTTAAG 2 cut(s) 128, 452
MspI CCGG 1 cut(s) 186
MspR9I CCNGG 2 cut(s) 187, 1340
MunI CAATTG 2 cut(s) 240, 1046
MvaI CCWGG 1 cut(s) 1340
MwoI GCNNNNNNNGC 2 cut(s) 527, 1283
NciI CCSGG 1 cut(s) 187
NcoI CCATGG 1 cut(s) 52
NheI GCTAGC 1 cut(s) 1094
NlaIII CATG 6 cut(s) 56, 127, 725, 832, 1222, 1539
NlaIV GGNNCC 4 cut(s) 339, 774, 1083, 1224
NmeAIII GCCGAG 1 cut(s) 1543
NmuCI GTSAC 2 cut(s) 171, 642
NspI RCATGY 1 cut(s) 725
PfeI GAWTC 7 cut(s) 179, 194, 382, 425, 764, 1003, 1396
PkrI GCNGC 4 cut(s) 413, 1250, 1516, 1558
PleI GAGTC 2 cut(s) 110, 178
PpsI GAGTC 2 cut(s) 110, 178
PpuMI RGGWCCY 3 cut(s) 34, 772, 1081
PsiI TTATAA 1 cut(s) 1106
Psp124BI GAGCTC 1 cut(s) 1288
Psp1406I AACGTT 1 cut(s) 464
Psp5II RGGWCCY 3 cut(s) 34, 772, 1081
Psp6I CCWGG 1 cut(s) 1338
PspFI CCCAGC 2 cut(s) 304, 1363
PspGI CCWGG 1 cut(s) 1338
PspN4I GGNNCC 4 cut(s) 339, 774, 1083, 1224
PspPI GGNCC 4 cut(s) 34, 772, 1081, 1222
PspPPI RGGWCCY 3 cut(s) 34, 772, 1081
PstI CTGCAG 1 cut(s) 1516
PstNI CAGNNNCTG 1 cut(s) 385
PsuI RGATCY 3 cut(s) 448, 497, 813
PvuII CAGCTG 2 cut(s) 296, 320
RsaI GTAC 6 cut(s) 238, 581, 744, 1065, 1124, 1464
RsaNI GTAC 6 cut(s) 237, 580, 743, 1064, 1123, 1463
RseI CAYNNNNRTG 1 cut(s) 707
SacI GAGCTC 1 cut(s) 1288
SaqAI TTAA 6 cut(s) 129, 269, 453, 510, 1317, 1532
SatI GCNGC 4 cut(s) 412, 1249, 1515, 1557
Sau96I GGNCC 4 cut(s) 34, 772, 1081, 1222
ScaI AGTACT 1 cut(s) 1124
SchI GAGTC 2 cut(s) 110, 179
ScrFI CCNGG 2 cut(s) 187, 1340
SduI GDGCHC 5 cut(s) 978, 1000, 1288, 1311, 1410
SfaNI GCATC 1 cut(s) 1235
SfcI CTRYAG 1 cut(s) 1512
SinI GGWCC 4 cut(s) 34, 772, 1081, 1222
SmiMI CAYNNNNRTG 1 cut(s) 707
SmlI CTYRAG 2 cut(s) 128, 452
SmoI CTYRAG 2 cut(s) 128, 452
SpeI ACTAGT 1 cut(s) 1193
SsiI CCGC 3 cut(s) 948, 1336, 1375
SspMI CTAG 4 cut(s) 176, 429, 1095, 1194
SstI GAGCTC 1 cut(s) 1288
StyD4I CCNGG 2 cut(s) 185, 1338
StyI CCWWGG 1 cut(s) 52
TaaI ACNGT 3 cut(s) 11, 1068, 1102
TaiI ACGT 2 cut(s) 90, 467
TaqI TCGA 1 cut(s) 27
TatI WGTACW 3 cut(s) 236, 1063, 1122
TfiI GAWTC 7 cut(s) 179, 194, 382, 425, 764, 1003, 1396
Tru1I TTAA 6 cut(s) 129, 269, 453, 510, 1317, 1532
Tru9I TTAA 6 cut(s) 129, 269, 453, 510, 1317, 1532
TscAI CASTG 5 cut(s) 229, 1032, 1105, 1397, 1410
TseFI GTSAC 2 cut(s) 171, 642
TseI GCWGC 4 cut(s) 411, 1248, 1514, 1556
Tsp45I GTSAC 2 cut(s) 171, 642
TspDTI ATGAA 5 cut(s) 80, 528, 630, 817, 1436
TspRI CASTG 5 cut(s) 229, 1032, 1105, 1397, 1410
Vha464I CTTAAG 2 cut(s) 128, 452
VpaK11BI GGWCC 4 cut(s) 34, 772, 1081, 1222
XapI RAATTY 5 cut(s) 135, 146, 824, 1032, 1507
XceI RCATGY 1 cut(s) 725
XcmI CCANNNNNNNNNTGG 1 cut(s) 239
XspI CTAG 4 cut(s) 176, 429, 1095, 1194
ZrmI AGTACT 1 cut(s) 1124
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.