Rh2BG047300

Aux IAA proteins are short-lived transcriptional factors that function as repressors of early auxin response genes at low auxin concentrations

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0001510
Physical Location & Seq
Reverse (-)
3346618 .. 3348455
1838 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG047300.1

Sequence Viewer

Length: 861 bp
ATGCTCTATTTATGTCTAAAATGTGCAGGTAGCAGAGTTGCGCGCGGGTTTGGATCTGATACGTACGTACCAGTTAAGAACAAGGAACAAATCCTACGCCGTACTTACACGTACTGGGCTCGCCGGAAAGCTGACGTGAACTTGTGGAATATGACGATGAATTACAAGGACACTGAGCTGACCTTGGGATTACCTGGTGGTGGAAGCCGTGGTTGTTCAACAGGTGTAGTGGTCTTCGGAGAGTTGATGAAGAGCTCGATGAAACGTGGGTTCATGGATACCGATGATGATCTCAACAACCTTGACCTGGAAAGCTCTAATGGCGAGCCTCGTGGCCAAGCTAAATTGGATGATGGGCCGTCGATGGAAGCAGCTAAGTACTCTTCCAGCAGTACCAGTACTACTGCCGTGAAAGCCCCAACTGCAAAGGGTCAAGTAGTTGGGTGGCCGCCGGTGAGATCGACGAGAAATAAGGCCTTGGACTCGAAGAACTTGAAGAACAACAGCAGCAGTAAATATGTGAAAGTGGGTGCTGATGGAGCTCCTTACCTGCGGAAAGTAGATTTAGAGATGTCTAAAAGTTACGAGGAGCTATTAACAGCCTTGGAGAAAATTTTCACTTCCTTCACCATAAACGGTAAGTACATGGAGGATGATAGGAAGCAACAGATGCTTTTTATGGAGCATGCAGTAGTGAATGGAACAGTGGAATATAATCAATATGTGCTGACATATGAAGATAAGGATAATGACTGGATGTTAGTTGGAGACGTTCCTTGGAATATTGGGTGCAGAATGTTTAAAGATTCGTGCAAGCGTATCCGGTTGATGAAAAGTTCAGAAGCTGCGGTCAGAAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0009719 GO:0009725 GO:0009733 GO:0010033 GO:0042221 GO:0050896
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
AUX_IAA

Protein Analysis

286

Amino Acids

32.09

Weight (kDa)

9.0

Isoelectric Point (pI)

39.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AUX_IAA PF02309 57 - 283 3.7e-55 AUX/IAA family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 17, 558
AccII CGCG 2 cut(s) 43, 45
AciI CCGC 4 cut(s) 45, 449, 553, 848
AclWI GGATC 1 cut(s) 61
AcoI YGGCCR 2 cut(s) 334, 446
AcsI RAATTY 1 cut(s) 612
AfaI GTAC 8 cut(s) 65, 69, 103, 113, 380, 394, 400, 644
AfiI CCNNNNNNNGG 2 cut(s) 200, 307
AflIII ACRYGT 1 cut(s) 108
AgsI TTSAA 2 cut(s) 219, 496
AjiI CACGTC 1 cut(s) 136
AjnI CCWGG 2 cut(s) 193, 306
AluBI AGCT 9 cut(s) 131, 178, 255, 315, 341, 374, 542, 592, 845
AluI AGCT 9 cut(s) 131, 178, 255, 315, 341, 374, 542, 592, 845
Alw21I GWGCWC 2 cut(s) 257, 544
Alw26I GTCTC 1 cut(s) 762
AlwI GGATC 1 cut(s) 61
AlwNI CAGNNNCTG 1 cut(s) 845
AoxI GGCC 4 cut(s) 334, 356, 446, 474
ApeKI GCWGC 3 cut(s) 371, 507, 845
ApoI RAATTY 1 cut(s) 612
Asp700I GAANNNNTTC 1 cut(s) 614
AspLEI GCGC 2 cut(s) 43, 45
AspS9I GGNCC 1 cut(s) 356
AsuHPI GGTGA 2 cut(s) 466, 619
BaeI ACNNNNGTAYC 4 cut(s) 51, 51, 84, 84
BalI TGGCCA 1 cut(s) 336
BanII GRGCYC 3 cut(s) 121, 257, 544
BauI CACGAG 1 cut(s) 330
BbsI GAAGAC 1 cut(s) 226
Bbv12I GWGCWC 2 cut(s) 257, 544
BbvI GCAGC 3 cut(s) 383, 519, 832
BccI CCATC 3 cut(s) 347, 358, 530
BceAI ACGGC 4 cut(s) 84, 192, 343, 392
BciT130I CCWGG 2 cut(s) 195, 308
BciVI GTATCC 2 cut(s) 271, 830
BcoDI GTCTC 1 cut(s) 762
BfuAI ACCTGC 2 cut(s) 17, 558
BfuI GTATCC 2 cut(s) 271, 830
BisI GCNGC 4 cut(s) 372, 449, 508, 846
BlsI GCNGC 4 cut(s) 373, 450, 509, 847
BmcAI AGTACT 2 cut(s) 380, 400
Bme1390I CCNGG 2 cut(s) 195, 308
BmgBI CACGTC 1 cut(s) 136
BmgT120I GGNCC 1 cut(s) 356
BmrFI CCNGG 2 cut(s) 195, 308
BmrI ACTGGG 1 cut(s) 124
BmsI GCATC 1 cut(s) 660
BmuI ACTGGG 1 cut(s) 124
BpiI GAAGAC 1 cut(s) 226
BsaAI YACGTR 3 cut(s) 63, 67, 111
BsaBI GATNNNNATC 1 cut(s) 288
BsaJI CCNNGG 5 cut(s) 183, 208, 477, 603, 776
BsaWI WCCGGW 1 cut(s) 822
Bsc4I CCNNNNNNNGG 2 cut(s) 200, 307
Bse118I RCCGGY 1 cut(s) 451
Bse1I ACTGG 4 cut(s) 71, 119, 396, 758
Bse8I GATNNNNATC 1 cut(s) 288
BseBI CCWGG 2 cut(s) 195, 308
BseDI CCNNGG 5 cut(s) 183, 208, 477, 603, 776
BseGI GGATG 3 cut(s) 355, 658, 762
BseJI GATNNNNATC 1 cut(s) 288
BseLI CCNNNNNNNGG 2 cut(s) 200, 307
BseMII CTCAG 1 cut(s) 165
BseNI ACTGG 4 cut(s) 71, 119, 396, 758
BsePI GCGCGC 1 cut(s) 41
BseRI GAGGAG 1 cut(s) 602
BseXI GCAGC 3 cut(s) 383, 519, 832
BsgI GTGCAG 2 cut(s) 45, 811
Bsh1236I CGCG 2 cut(s) 43, 45
BshFI GGCC 4 cut(s) 336, 358, 448, 476
BsiHKAI GWGCWC 2 cut(s) 257, 544
BsiSI CCGG 3 cut(s) 124, 452, 823
BsiWI CGTACG 1 cut(s) 63
BslI CCNNNNNNNGG 2 cut(s) 200, 307
BsmAI GTCTC 1 cut(s) 762
BsmBI CGTCTC 1 cut(s) 762
BsnI GGCC 4 cut(s) 336, 358, 448, 476
Bsp1286I GDGCHC 3 cut(s) 121, 257, 544
Bsp143I GATC 3 cut(s) 53, 289, 458
BspACI CCGC 4 cut(s) 45, 449, 553, 848
BspANI GGCC 4 cut(s) 336, 358, 448, 476
BspCNI CTCAG 1 cut(s) 166
BspFNI CGCG 2 cut(s) 43, 45
BspMI ACCTGC 2 cut(s) 17, 558
BspPI GGATC 1 cut(s) 61
BspQI GCTCTTC 1 cut(s) 245
BsrFI RCCGGY 1 cut(s) 451
BsrI ACTGG 4 cut(s) 71, 119, 396, 758
BssAI RCCGGY 1 cut(s) 451
BssECI CCNNGG 5 cut(s) 183, 208, 477, 603, 776
BssHII GCGCGC 1 cut(s) 41
BssMI GATC 3 cut(s) 53, 289, 458
BssSI CACGAG 1 cut(s) 330
BssT1I CCWWGG 4 cut(s) 183, 477, 603, 776
Bst2BI CACGAG 1 cut(s) 330
Bst2UI CCWGG 2 cut(s) 195, 308
Bst4CI ACNGT 2 cut(s) 638, 706
Bst6I CTCTTC 2 cut(s) 245, 388
BstAPI GCANNNNNTGC 1 cut(s) 670
BstBAI YACGTR 3 cut(s) 63, 67, 111
BstC8I GCNNGC 5 cut(s) 43, 121, 326, 687, 815
BstDEI CTNAG 2 cut(s) 174, 375
BstDSI CCRYGG 1 cut(s) 208
BstF5I GGATG 3 cut(s) 355, 658, 762
BstFNI CGCG 2 cut(s) 43, 45
BstHHI GCGC 2 cut(s) 43, 45
BstKTI GATC 3 cut(s) 56, 292, 461
BstMAI GTCTC 1 cut(s) 762
BstMBI GATC 3 cut(s) 53, 289, 458
BstMWI GCNNNNNNNGC 5 cut(s) 321, 413, 422, 539, 670
BstNI CCWGG 2 cut(s) 195, 308
BstNSI RCATGY 1 cut(s) 689
BstSCI CCNGG 2 cut(s) 193, 306
BstSNI TACGTA 2 cut(s) 63, 67
BstUI CGCG 2 cut(s) 43, 45
BstV1I GCAGC 3 cut(s) 383, 519, 832
BstV2I GAAGAC 1 cut(s) 226
BstX2I RGATCY 1 cut(s) 53
BstYI RGATCY 1 cut(s) 53
BsuI GTATCC 2 cut(s) 271, 830
BsuRI GGCC 4 cut(s) 336, 358, 448, 476
BtgI CCRYGG 1 cut(s) 208
BtrI CACGTC 1 cut(s) 136
BtsCI GGATG 3 cut(s) 355, 658, 762
BtsIMutI CAGTG 2 cut(s) 171, 711
BveI ACCTGC 2 cut(s) 17, 558
Cac8I GCNNGC 5 cut(s) 43, 121, 326, 687, 815
CaiI CAGNNNCTG 1 cut(s) 845
CfoI GCGC 2 cut(s) 43, 45
Cfr10I RCCGGY 1 cut(s) 451
Cfr13I GGNCC 1 cut(s) 356
CsiI ACCWGGT 1 cut(s) 193
Csp6I GTAC 8 cut(s) 64, 68, 102, 112, 379, 393, 399, 643
CviAII CATG 3 cut(s) 274, 646, 686
CviQI GTAC 8 cut(s) 64, 68, 102, 112, 379, 393, 399, 643
DdeI CTNAG 2 cut(s) 174, 375
DpnI GATC 3 cut(s) 55, 291, 460
DpnII GATC 3 cut(s) 53, 289, 458
DraI TTTAAA 1 cut(s) 802
EaeI YGGCCR 2 cut(s) 334, 446
Eam1104I CTCTTC 2 cut(s) 245, 388
EarI CTCTTC 2 cut(s) 245, 388
Ecl136II GAGCTC 2 cut(s) 255, 542
Eco105I TACGTA 2 cut(s) 63, 67
Eco130I CCWWGG 4 cut(s) 183, 477, 603, 776
Eco147I AGGCCT 1 cut(s) 476
Eco24I GRGCYC 3 cut(s) 121, 257, 544
Eco53kI GAGCTC 2 cut(s) 255, 542
EcoICRI GAGCTC 2 cut(s) 255, 542
EcoRII CCWGG 2 cut(s) 193, 306
EcoT14I CCWWGG 4 cut(s) 183, 477, 603, 776
EcoT38I GRGCYC 3 cut(s) 121, 257, 544
ErhI CCWWGG 4 cut(s) 183, 477, 603, 776
Esp3I CGTCTC 1 cut(s) 762
FaeI CATG 3 cut(s) 277, 649, 689
FatI CATG 3 cut(s) 273, 645, 685
FauI CCCGC 1 cut(s) 38
FauNDI CATATG 1 cut(s) 733
Fnu4HI GCNGC 4 cut(s) 372, 449, 508, 846
FokI GGATG 3 cut(s) 362, 665, 769
FriOI GRGCYC 3 cut(s) 121, 257, 544
Fsp4HI GCNGC 4 cut(s) 372, 449, 508, 846
GlaI GCGC 2 cut(s) 42, 44
GluI GCNGC 4 cut(s) 372, 449, 508, 846
HaeIII GGCC 4 cut(s) 336, 358, 448, 476
HapII CCGG 3 cut(s) 124, 452, 823
HhaI GCGC 2 cut(s) 43, 45
Hin1II CATG 3 cut(s) 277, 649, 689
Hin6I GCGC 2 cut(s) 41, 43
HinP1I GCGC 2 cut(s) 41, 43
HinfI GANTC 2 cut(s) 482, 806
HpaII CCGG 3 cut(s) 124, 452, 823
HphI GGTGA 2 cut(s) 466, 619
Hpy166II GTNNAC 1 cut(s) 139
Hpy188I TCNGA 4 cut(s) 58, 239, 841, 854
Hpy8I GTNNAC 1 cut(s) 139
Hpy99I CGWCG 2 cut(s) 364, 466
HpyAV CCTTC 1 cut(s) 634
HpyCH4III ACNGT 2 cut(s) 638, 706
HpyCH4IV ACGT 6 cut(s) 62, 66, 110, 135, 265, 771
HpyCH4V TGCA 5 cut(s) 26, 425, 689, 792, 813
HpyF10VI GCNNNNNNNGC 5 cut(s) 321, 413, 422, 539, 670
HpyF3I CTNAG 2 cut(s) 174, 375
HpySE526I ACGT 6 cut(s) 62, 66, 110, 135, 265, 771
Hsp92II CATG 3 cut(s) 277, 649, 689
HspAI GCGC 2 cut(s) 41, 43
Kzo9I GATC 3 cut(s) 53, 289, 458
LguI GCTCTTC 1 cut(s) 245
LmnI GCTCC 4 cut(s) 539, 547, 589, 682
Lsp1109I GCAGC 3 cut(s) 383, 519, 832
LweI GCATC 1 cut(s) 660
MabI ACCWGGT 1 cut(s) 193
MaeII ACGT 6 cut(s) 62, 66, 110, 135, 265, 771
MaeIII GTNAC 1 cut(s) 581
MalI GATC 3 cut(s) 55, 291, 460
MboI GATC 3 cut(s) 53, 289, 458
MboII GAAGA 6 cut(s) 226, 262, 375, 499, 508, 749
MflI RGATCY 1 cut(s) 53
MhlI GDGCHC 3 cut(s) 121, 257, 544
MlsI TGGCCA 1 cut(s) 336
MluCI AATT 4 cut(s) 160, 344, 612, 856
MluNI TGGCCA 1 cut(s) 336
MlyI GAGTC 1 cut(s) 476
MmeI TCCRAC 1 cut(s) 745
MnlI CCTC 3 cut(s) 339, 580, 643
Mox20I TGGCCA 1 cut(s) 336
MroXI GAANNNNTTC 1 cut(s) 614
MscI TGGCCA 1 cut(s) 336
MseI TTAA 3 cut(s) 75, 596, 801
Msp20I TGGCCA 1 cut(s) 336
MspI CCGG 3 cut(s) 124, 452, 823
MspR9I CCNGG 2 cut(s) 195, 308
MvaI CCWGG 2 cut(s) 195, 308
MvnI CGCG 2 cut(s) 43, 45
MwoI GCNNNNNNNGC 5 cut(s) 321, 413, 422, 539, 670
NdeI CATATG 1 cut(s) 733
NdeII GATC 3 cut(s) 53, 289, 458
NlaIII CATG 3 cut(s) 277, 649, 689
NspI RCATGY 1 cut(s) 689
PaeI GCATGC 1 cut(s) 689
PauI GCGCGC 1 cut(s) 41
PceI AGGCCT 1 cut(s) 476
PciSI GCTCTTC 1 cut(s) 245
PdmI GAANNNNTTC 1 cut(s) 614
PfeI GAWTC 1 cut(s) 806
Pfl23II CGTACG 1 cut(s) 63
PkrI GCNGC 4 cut(s) 373, 450, 509, 847
PleI GAGTC 1 cut(s) 476
PpsI GAGTC 1 cut(s) 476
Ppu21I YACGTR 3 cut(s) 63, 67, 111
Psp124BI GAGCTC 2 cut(s) 257, 544
Psp6I CCWGG 2 cut(s) 193, 306
PspGI CCWGG 2 cut(s) 193, 306
PspLI CGTACG 1 cut(s) 63
PspPI GGNCC 1 cut(s) 356
PsrI GAACNNNNNNTAC 2 cut(s) 78, 110
PstNI CAGNNNCTG 1 cut(s) 845
PsuI RGATCY 1 cut(s) 53
PteI GCGCGC 1 cut(s) 41
RsaI GTAC 8 cut(s) 65, 69, 103, 113, 380, 394, 400, 644
RsaNI GTAC 8 cut(s) 64, 68, 102, 112, 379, 393, 399, 643
SacI GAGCTC 2 cut(s) 257, 544
SapI GCTCTTC 1 cut(s) 245
SaqAI TTAA 3 cut(s) 75, 596, 801
SatI GCNGC 4 cut(s) 372, 449, 508, 846
Sau3AI GATC 3 cut(s) 53, 289, 458
Sau96I GGNCC 1 cut(s) 356
ScaI AGTACT 2 cut(s) 380, 400
SchI GAGTC 1 cut(s) 476
ScrFI CCNGG 2 cut(s) 195, 308
SduI GDGCHC 3 cut(s) 121, 257, 544
SexAI ACCWGGT 1 cut(s) 193
SfaNI GCATC 1 cut(s) 660
SgrAI CRCCGGYG 1 cut(s) 451
SnaBI TACGTA 2 cut(s) 63, 67
SphI GCATGC 1 cut(s) 689
Sse9I AATT 4 cut(s) 160, 344, 612, 856
SseBI AGGCCT 1 cut(s) 476
SsiI CCGC 4 cut(s) 45, 449, 553, 848
SspI AATATT 1 cut(s) 784
SstI GAGCTC 2 cut(s) 257, 544
StuI AGGCCT 1 cut(s) 476
StyD4I CCNGG 2 cut(s) 193, 306
StyI CCWWGG 4 cut(s) 183, 477, 603, 776
TaaI ACNGT 2 cut(s) 638, 706
TaiI ACGT 6 cut(s) 65, 69, 113, 138, 268, 774
TaqI TCGA 4 cut(s) 257, 362, 461, 485
TasI AATT 4 cut(s) 160, 344, 612, 856
TatI WGTACW 3 cut(s) 378, 398, 642
TauI GCSGC 1 cut(s) 451
TfiI GAWTC 1 cut(s) 806
Tru1I TTAA 3 cut(s) 75, 596, 801
Tru9I TTAA 3 cut(s) 75, 596, 801
TscAI CASTG 2 cut(s) 178, 711
TseI GCWGC 3 cut(s) 371, 507, 845
TspDTI ATGAA 6 cut(s) 173, 262, 263, 275, 750, 845
TspRI CASTG 2 cut(s) 178, 711
XapI RAATTY 1 cut(s) 612
XceI RCATGY 1 cut(s) 689
XmnI GAANNNNTTC 1 cut(s) 614
ZrmI AGTACT 2 cut(s) 380, 400
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.