The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 1016 |
| AasI | GACNNNNNNGTC | 2 cut(s) | 106, 1223 |
| AatII | GACGTC | 1 cut(s) | 263 |
| Acc16I | TGCGCA | 1 cut(s) | 1239 |
| AccB1I | GGYRCC | 2 cut(s) | 125, 238 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 1396 |
| AccBSI | CCGCTC | 1 cut(s) | 87 |
| AccI | GTMKAC | 1 cut(s) | 636 |
| AccII | CGCG | 4 cut(s) | 31, 70, 493, 614 |
| AclI | AACGTT | 1 cut(s) | 311 |
| AclWI | GGATC | 3 cut(s) | 129, 677, 690 |
| AcoI | YGGCCR | 3 cut(s) | 219, 551, 1037 |
| AcsI | RAATTY | 3 cut(s) | 337, 587, 1115 |
| AcyI | GRCGYC | 1 cut(s) | 260 |
| AfiI | CCNNNNNNNGG | 9 cut(s) | 54, 89, 90, 96, 121, 1180, 1284, 1396, 1487 |
| AgeI | ACCGGT | 1 cut(s) | 486 |
| AgsI | TTSAA | 5 cut(s) | 43, 247, 706, 877, 1030 |
| AjiI | CACGTC | 1 cut(s) | 253 |
| AjnI | CCWGG | 2 cut(s) | 548, 1179 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 322, 354 |
| AluBI | AGCT | 5 cut(s) | 153, 1187, 1369, 1419, 1469 |
| AluI | AGCT | 5 cut(s) | 153, 1187, 1369, 1419, 1469 |
| Alw21I | GWGCWC | 1 cut(s) | 888 |
| Alw26I | GTCTC | 3 cut(s) | 794, 802, 1478 |
| AlwI | GGATC | 3 cut(s) | 129, 677, 690 |
| Ama87I | CYCGRG | 2 cut(s) | 154, 160 |
| ApaI | GGGCCC | 2 cut(s) | 161, 1198 |
| ApeKI | GCWGC | 5 cut(s) | 580, 1158, 1366, 1424, 1466 |
| ApoI | RAATTY | 3 cut(s) | 337, 587, 1115 |
| ArsI | GACNNNNNNTTYG | 4 cut(s) | 173, 205, 473, 505 |
| AseI | ATTAAT | 1 cut(s) | 456 |
| AsiGI | ACCGGT | 1 cut(s) | 486 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 278 |
| AspA2I | CCTAGG | 1 cut(s) | 686 |
| AspLEI | GCGC | 1 cut(s) | 1240 |
| AspS9I | GGNCC | 7 cut(s) | 104, 157, 158, 325, 1132, 1194, 1195 |
| AsuC2I | CCSGG | 3 cut(s) | 91, 161, 162 |
| AsuHPI | GGTGA | 5 cut(s) | 304, 524, 1075, 1088, 1219 |
| AsuNHI | GCTAGC | 1 cut(s) | 209 |
| AvaI | CYCGRG | 2 cut(s) | 154, 160 |
| AvaII | GGWCC | 1 cut(s) | 325 |
| AvrII | CCTAGG | 1 cut(s) | 686 |
| BaeGI | GKGCMC | 3 cut(s) | 128, 161, 1198 |
| BamHI | GGATCC | 1 cut(s) | 682 |
| BanI | GGYRCC | 2 cut(s) | 125, 238 |
| BanII | GRGCYC | 3 cut(s) | 161, 534, 1198 |
| BbsI | GAAGAC | 1 cut(s) | 713 |
| Bbv12I | GWGCWC | 1 cut(s) | 888 |
| BbvI | GCAGC | 5 cut(s) | 567, 1145, 1378, 1436, 1478 |
| BccI | CCATC | 1 cut(s) | 1351 |
| BceAI | ACGGC | 2 cut(s) | 91, 226 |
| BcgI | CGANNNNNNTGC | 4 cut(s) | 135, 169, 384, 418 |
| BciT130I | CCWGG | 2 cut(s) | 550, 1181 |
| BcnI | CCSGG | 3 cut(s) | 91, 161, 162 |
| BcoDI | GTCTC | 3 cut(s) | 794, 802, 1478 |
| BfaI | CTAG | 4 cut(s) | 210, 687, 1005, 1470 |
| BfmI | CTRYAG | 2 cut(s) | 439, 1515 |
| BisI | GCNGC | 8 cut(s) | 29, 68, 554, 581, 1159, 1367, 1425, 1467 |
| BlnI | CCTAGG | 1 cut(s) | 686 |
| BlsI | GCNGC | 8 cut(s) | 30, 69, 555, 582, 1160, 1368, 1426, 1468 |
| Bme1390I | CCNGG | 5 cut(s) | 91, 161, 162, 550, 1181 |
| Bme18I | GGWCC | 1 cut(s) | 325 |
| BmeT110I | CYCGRG | 2 cut(s) | 154, 160 |
| BmgBI | CACGTC | 1 cut(s) | 253 |
| BmgT120I | GGNCC | 7 cut(s) | 104, 157, 158, 325, 1132, 1194, 1195 |
| BmrFI | CCNGG | 5 cut(s) | 91, 161, 162, 550, 1181 |
| BmrI | ACTGGG | 2 cut(s) | 111, 408 |
| BmtI | GCTAGC | 1 cut(s) | 213 |
| BmuI | ACTGGG | 2 cut(s) | 111, 408 |
| BpiI | GAAGAC | 1 cut(s) | 713 |
| BplI | GAGNNNNNCTC | 4 cut(s) | 1274, 1306, 1276, 1308 |
| BpmI | CTGGAG | 3 cut(s) | 854, 1363, 1460 |
| BpuEI | CTTGAG | 1 cut(s) | 683 |
| BpuMI | CCSGG | 3 cut(s) | 91, 161, 162 |
| BsaHI | GRCGYC | 1 cut(s) | 260 |
| BsaJI | CCNNGG | 7 cut(s) | 29, 68, 115, 160, 678, 686, 1278 |
| BsaWI | WCCGGW | 2 cut(s) | 486, 1487 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 983, 1013 |
| Bsc4I | CCNNNNNNNGG | 9 cut(s) | 54, 89, 90, 96, 121, 1180, 1284, 1396, 1487 |
| Bse118I | RCCGGY | 1 cut(s) | 486 |
| Bse1I | ACTGG | 4 cut(s) | 106, 403, 1307, 1481 |
| Bse3DI | GCAATG | 1 cut(s) | 368 |
| BseBI | CCWGG | 2 cut(s) | 550, 1181 |
| BseDI | CCNNGG | 7 cut(s) | 29, 68, 115, 160, 678, 686, 1278 |
| BseGI | GGATG | 1 cut(s) | 1366 |
| BseLI | CCNNNNNNNGG | 9 cut(s) | 54, 89, 90, 96, 121, 1180, 1284, 1396, 1487 |
| BseMI | GCAATG | 1 cut(s) | 368 |
| BseMII | CTCAG | 2 cut(s) | 31, 816 |
| BseNI | ACTGG | 4 cut(s) | 106, 403, 1307, 1481 |
| BseRI | GAGGAG | 1 cut(s) | 896 |
| BseSI | GKGCMC | 3 cut(s) | 128, 161, 1198 |
| BseXI | GCAGC | 5 cut(s) | 567, 1145, 1378, 1436, 1478 |
| Bsh1236I | CGCG | 4 cut(s) | 31, 70, 493, 614 |
| Bsh1285I | CGRYCG | 1 cut(s) | 491 |
| BshNI | GGYRCC | 2 cut(s) | 125, 238 |
| BshTI | ACCGGT | 1 cut(s) | 486 |
| BsiEI | CGRYCG | 1 cut(s) | 491 |
| BsiHKAI | GWGCWC | 1 cut(s) | 888 |
| BsiHKCI | CYCGRG | 2 cut(s) | 154, 160 |
| BsiSI | CCGG | 8 cut(s) | 59, 90, 97, 161, 206, 222, 487, 1488 |
| BslI | CCNNNNNNNGG | 9 cut(s) | 54, 89, 90, 96, 121, 1180, 1284, 1396, 1487 |
| BsmAI | GTCTC | 3 cut(s) | 794, 802, 1478 |
| BsmI | GAATGC | 2 cut(s) | 178, 1468 |
| BsoBI | CYCGRG | 2 cut(s) | 154, 160 |
| Bsp120I | GGGCCC | 2 cut(s) | 157, 1194 |
| Bsp1286I | GDGCHC | 5 cut(s) | 128, 161, 534, 888, 1198 |
| Bsp143I | GATC | 2 cut(s) | 134, 682 |
| BspCNI | CTCAG | 2 cut(s) | 30, 817 |
| BspFNI | CGCG | 4 cut(s) | 31, 70, 493, 614 |
| BspOI | GCTAGC | 1 cut(s) | 213 |
| BspPI | GGATC | 3 cut(s) | 129, 677, 690 |
| BspT107I | GGYRCC | 2 cut(s) | 125, 238 |
| BsrBI | CCGCTC | 1 cut(s) | 87 |
| BsrDI | GCAATG | 1 cut(s) | 368 |
| BsrFI | RCCGGY | 1 cut(s) | 486 |
| BsrI | ACTGG | 4 cut(s) | 106, 403, 1307, 1481 |
| BssAI | RCCGGY | 1 cut(s) | 486 |
| BssECI | CCNNGG | 7 cut(s) | 29, 68, 115, 160, 678, 686, 1278 |
| BssMI | GATC | 2 cut(s) | 134, 682 |
| BssNI | GRCGYC | 1 cut(s) | 260 |
| BssT1I | CCWWGG | 2 cut(s) | 686, 1278 |
| Bst2UI | CCWGG | 2 cut(s) | 550, 1181 |
| Bst4CI | ACNGT | 5 cut(s) | 143, 957, 1169, 1247, 1455 |
| Bst6I | CTCTTC | 2 cut(s) | 284, 1080 |
| BstACI | GRCGYC | 1 cut(s) | 260 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 370 |
| BstC8I | GCNNGC | 4 cut(s) | 211, 419, 423, 1156 |
| BstDEI | CTNAG | 3 cut(s) | 17, 333, 825 |
| BstDSI | CCRYGG | 2 cut(s) | 29, 68 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 1282 |
| BstF5I | GGATG | 1 cut(s) | 1366 |
| BstFNI | CGCG | 4 cut(s) | 31, 70, 493, 614 |
| BstHHI | GCGC | 1 cut(s) | 1240 |
| BstKTI | GATC | 2 cut(s) | 137, 685 |
| BstMAI | GTCTC | 3 cut(s) | 794, 802, 1478 |
| BstMBI | GATC | 2 cut(s) | 134, 682 |
| BstMCI | CGRYCG | 1 cut(s) | 491 |
| BstNI | CCWGG | 2 cut(s) | 550, 1181 |
| BstNSI | RCATGY | 2 cut(s) | 190, 1158 |
| BstSCI | CCNGG | 5 cut(s) | 89, 159, 160, 548, 1179 |
| BstSFI | CTRYAG | 2 cut(s) | 439, 1515 |
| BstSLI | GKGCMC | 3 cut(s) | 128, 161, 1198 |
| BstUI | CGCG | 4 cut(s) | 31, 70, 493, 614 |
| BstV1I | GCAGC | 5 cut(s) | 567, 1145, 1378, 1436, 1478 |
| BstV2I | GAAGAC | 1 cut(s) | 713 |
| BstX2I | RGATCY | 1 cut(s) | 682 |
| BstYI | RGATCY | 1 cut(s) | 682 |
| BtgI | CCRYGG | 2 cut(s) | 29, 68 |
| BtrI | CACGTC | 1 cut(s) | 253 |
| BtsCI | GGATG | 1 cut(s) | 1366 |
| BtsI | GCAGTG | 1 cut(s) | 1494 |
| BtsIMutI | CAGTG | 2 cut(s) | 1300, 1494 |
| Cac8I | GCNNGC | 4 cut(s) | 211, 419, 423, 1156 |
| CfoI | GCGC | 1 cut(s) | 1240 |
| Cfr10I | RCCGGY | 1 cut(s) | 486 |
| Cfr13I | GGNCC | 7 cut(s) | 104, 157, 158, 325, 1132, 1194, 1195 |
| Cfr42I | CCGCGG | 2 cut(s) | 32, 71 |
| Cfr9I | CCCGGG | 1 cut(s) | 160 |
| CseI | GACGC | 2 cut(s) | 620, 958 |
| CspAI | ACCGGT | 1 cut(s) | 486 |
| CviAII | CATG | 9 cut(s) | 187, 535, 739, 1108, 1129, 1155, 1385, 1396, 1446 |
| DdeI | CTNAG | 3 cut(s) | 17, 333, 825 |
| DpnI | GATC | 2 cut(s) | 136, 684 |
| DpnII | GATC | 2 cut(s) | 134, 682 |
| DraI | TTTAAA | 1 cut(s) | 1332 |
| DrdI | GACNNNNNNGTC | 2 cut(s) | 106, 1223 |
| DseDI | GACNNNNNNGTC | 2 cut(s) | 106, 1223 |
| EaeI | YGGCCR | 3 cut(s) | 219, 551, 1037 |
| Eam1104I | CTCTTC | 2 cut(s) | 284, 1080 |
| EarI | CTCTTC | 2 cut(s) | 284, 1080 |
| Eco130I | CCWWGG | 2 cut(s) | 686, 1278 |
| Eco147I | AGGCCT | 1 cut(s) | 38 |
| Eco24I | GRGCYC | 3 cut(s) | 161, 534, 1198 |
| Eco47I | GGWCC | 1 cut(s) | 325 |
| Eco88I | CYCGRG | 2 cut(s) | 154, 160 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 1282 |
| EcoO109I | RGGNCCY | 2 cut(s) | 1194, 1195 |
| EcoRI | GAATTC | 1 cut(s) | 337 |
| EcoRII | CCWGG | 2 cut(s) | 548, 1179 |
| EcoT14I | CCWWGG | 2 cut(s) | 686, 1278 |
| EcoT38I | GRGCYC | 3 cut(s) | 161, 534, 1198 |
| ErhI | CCWWGG | 2 cut(s) | 686, 1278 |
| FaeI | CATG | 9 cut(s) | 190, 538, 742, 1111, 1132, 1158, 1388, 1399, 1449 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 1302, 1334, 1403, 1435 |
| FatI | CATG | 9 cut(s) | 186, 534, 738, 1107, 1128, 1154, 1384, 1395, 1445 |
| FauI | CCCGC | 6 cut(s) | 92, 113, 483, 502, 553, 851 |
| FblI | GTMKAC | 1 cut(s) | 636 |
| Fnu4HI | GCNGC | 8 cut(s) | 29, 68, 554, 581, 1159, 1367, 1425, 1467 |
| FokI | GGATG | 1 cut(s) | 1373 |
| FriOI | GRGCYC | 3 cut(s) | 161, 534, 1198 |
| Fsp4HI | GCNGC | 8 cut(s) | 29, 68, 554, 581, 1159, 1367, 1425, 1467 |
| FspBI | CTAG | 4 cut(s) | 210, 687, 1005, 1470 |
| FspI | TGCGCA | 1 cut(s) | 1239 |
| GlaI | GCGC | 1 cut(s) | 1239 |
| GluI | GCNGC | 8 cut(s) | 29, 68, 554, 581, 1159, 1367, 1425, 1467 |
| GsuI | CTGGAG | 3 cut(s) | 854, 1363, 1460 |
| HapII | CCGG | 8 cut(s) | 59, 90, 97, 161, 206, 222, 487, 1488 |
| HgaI | GACGC | 2 cut(s) | 620, 958 |
| HhaI | GCGC | 1 cut(s) | 1240 |
| Hin1I | GRCGYC | 1 cut(s) | 260 |
| Hin1II | CATG | 9 cut(s) | 190, 538, 742, 1111, 1132, 1158, 1388, 1399, 1449 |
| Hin6I | GCGC | 1 cut(s) | 1238 |
| HinP1I | GCGC | 1 cut(s) | 1238 |
| HincII | GTYRAC | 1 cut(s) | 1105 |
| HindII | GTYRAC | 1 cut(s) | 1105 |
| HindIII | AAGCTT | 1 cut(s) | 1417 |
| HinfI | GANTC | 7 cut(s) | 62, 225, 411, 674, 913, 934, 1097 |
| HpaI | GTTAAC | 1 cut(s) | 1105 |
| HpaII | CCGG | 8 cut(s) | 59, 90, 97, 161, 206, 222, 487, 1488 |
| HphI | GGTGA | 5 cut(s) | 304, 524, 1075, 1088, 1219 |
| Hpy166II | GTNNAC | 4 cut(s) | 23, 385, 637, 1105 |
| Hpy188I | TCNGA | 7 cut(s) | 49, 265, 467, 679, 1056, 1122, 1340 |
| Hpy188III | TCNNGA | 2 cut(s) | 592, 608 |
| Hpy8I | GTNNAC | 4 cut(s) | 23, 385, 637, 1105 |
| Hpy99I | CGWCG | 3 cut(s) | 53, 262, 265 |
| HpyAV | CCTTC | 3 cut(s) | 28, 982, 1117 |
| HpyCH4III | ACNGT | 5 cut(s) | 143, 957, 1169, 1247, 1455 |
| HpyCH4IV | ACGT | 3 cut(s) | 252, 260, 311 |
| HpyCH4V | TGCA | 7 cut(s) | 146, 562, 623, 718, 922, 1161, 1466 |
| HpyF3I | CTNAG | 3 cut(s) | 17, 333, 825 |
| HpySE526I | ACGT | 3 cut(s) | 252, 260, 311 |
| Hsp92I | GRCGYC | 1 cut(s) | 260 |
| Hsp92II | CATG | 9 cut(s) | 190, 538, 742, 1111, 1132, 1158, 1388, 1399, 1449 |
| HspAI | GCGC | 1 cut(s) | 1238 |
| KspAI | GTTAAC | 1 cut(s) | 1105 |
| KspI | CCGCGG | 2 cut(s) | 32, 71 |
| Kzo9I | GATC | 2 cut(s) | 134, 682 |
| LmnI | GCTCC | 4 cut(s) | 92, 93, 883, 991 |
| Lsp1109I | GCAGC | 5 cut(s) | 567, 1145, 1378, 1436, 1478 |
| MaeI | CTAG | 4 cut(s) | 210, 687, 1005, 1470 |
| MaeII | ACGT | 3 cut(s) | 252, 260, 311 |
| MaeIII | GTNAC | 6 cut(s) | 307, 592, 970, 1225, 1483, 1507 |
| MalI | GATC | 2 cut(s) | 136, 684 |
| MbiI | CCGCTC | 1 cut(s) | 87 |
| MboI | GATC | 2 cut(s) | 134, 682 |
| MboII | GAAGA | 8 cut(s) | 271, 280, 591, 628, 718, 943, 1097, 1415 |
| MflI | RGATCY | 1 cut(s) | 682 |
| MhlI | GDGCHC | 5 cut(s) | 128, 161, 534, 888, 1198 |
| MluCI | AATT | 9 cut(s) | 11, 337, 481, 575, 587, 1115, 1233, 1254, 1321 |
| MlyI | GAGTC | 1 cut(s) | 71 |
| MmeI | TCCRAC | 4 cut(s) | 72, 162, 243, 976 |
| MroXI | GAANNNNTTC | 1 cut(s) | 278 |
| MseI | TTAA | 9 cut(s) | 357, 456, 585, 1104, 1164, 1232, 1320, 1331, 1537 |
| MspA1I | CMGCKG | 2 cut(s) | 31, 70 |
| MspI | CCGG | 8 cut(s) | 59, 90, 97, 161, 206, 222, 487, 1488 |
| MspR9I | CCNGG | 5 cut(s) | 91, 161, 162, 550, 1181 |
| Mva1269I | GAATGC | 2 cut(s) | 178, 1468 |
| MvaI | CCWGG | 2 cut(s) | 550, 1181 |
| MvnI | CGCG | 4 cut(s) | 31, 70, 493, 614 |
| NciI | CCSGG | 3 cut(s) | 91, 161, 162 |
| NdeII | GATC | 2 cut(s) | 134, 682 |
| NheI | GCTAGC | 1 cut(s) | 209 |
| NlaIII | CATG | 9 cut(s) | 190, 538, 742, 1111, 1132, 1158, 1388, 1399, 1449 |
| NmeAIII | GCCGAG | 1 cut(s) | 96 |
| NmuCI | GTSAC | 3 cut(s) | 592, 970, 1225 |
| NsbI | TGCGCA | 1 cut(s) | 1239 |
| NspI | RCATGY | 2 cut(s) | 190, 1158 |
| PaeI | GCATGC | 1 cut(s) | 1158 |
| PceI | AGGCCT | 1 cut(s) | 38 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 71 |
| PctI | GAATGC | 2 cut(s) | 178, 1468 |
| PdmI | GAANNNNTTC | 1 cut(s) | 278 |
| PfeI | GAWTC | 6 cut(s) | 225, 411, 674, 913, 934, 1097 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 1396 |
| PfoI | TCCNGGA | 1 cut(s) | 89 |
| PinAI | ACCGGT | 1 cut(s) | 486 |
| PkrI | GCNGC | 8 cut(s) | 30, 69, 555, 582, 1160, 1368, 1426, 1468 |
| PleI | GAGTC | 1 cut(s) | 70 |
| PpsI | GAGTC | 1 cut(s) | 70 |
| PshBI | ATTAAT | 1 cut(s) | 456 |
| PsiI | TTATAA | 1 cut(s) | 1016 |
| Psp1406I | AACGTT | 1 cut(s) | 311 |
| Psp6I | CCWGG | 2 cut(s) | 548, 1179 |
| PspGI | CCWGG | 2 cut(s) | 548, 1179 |
| PspOMI | GGGCCC | 2 cut(s) | 157, 1194 |
| PspPI | GGNCC | 7 cut(s) | 104, 157, 158, 325, 1132, 1194, 1195 |
| PsuI | RGATCY | 1 cut(s) | 682 |
| SacII | CCGCGG | 2 cut(s) | 32, 71 |
| SaqAI | TTAA | 9 cut(s) | 357, 456, 585, 1104, 1164, 1232, 1320, 1331, 1537 |
| SatI | GCNGC | 8 cut(s) | 29, 68, 554, 581, 1159, 1367, 1425, 1467 |
| Sau3AI | GATC | 2 cut(s) | 134, 682 |
| Sau96I | GGNCC | 7 cut(s) | 104, 157, 158, 325, 1132, 1194, 1195 |
| SchI | GAGTC | 1 cut(s) | 71 |
| ScrFI | CCNGG | 5 cut(s) | 91, 161, 162, 550, 1181 |
| SduI | GDGCHC | 5 cut(s) | 128, 161, 534, 888, 1198 |
| SfcI | CTRYAG | 2 cut(s) | 439, 1515 |
| Sfr303I | CCGCGG | 2 cut(s) | 32, 71 |
| SgrBI | CCGCGG | 2 cut(s) | 32, 71 |
| SinI | GGWCC | 1 cut(s) | 325 |
| SmaI | CCCGGG | 1 cut(s) | 162 |
| SmlI | CTYRAG | 2 cut(s) | 5, 662 |
| SmoI | CTYRAG | 2 cut(s) | 5, 662 |
| SphI | GCATGC | 1 cut(s) | 1158 |
| Sse9I | AATT | 9 cut(s) | 11, 337, 481, 575, 587, 1115, 1233, 1254, 1321 |
| SseBI | AGGCCT | 1 cut(s) | 38 |
| SspI | AATATT | 1 cut(s) | 1034 |
| SspMI | CTAG | 4 cut(s) | 210, 687, 1005, 1470 |
| StuI | AGGCCT | 1 cut(s) | 38 |
| StyD4I | CCNGG | 5 cut(s) | 89, 159, 160, 548, 1179 |
| StyI | CCWWGG | 2 cut(s) | 686, 1278 |
| TaaI | ACNGT | 5 cut(s) | 143, 957, 1169, 1247, 1455 |
| TaiI | ACGT | 3 cut(s) | 255, 263, 314 |
| TaqI | TCGA | 6 cut(s) | 133, 228, 341, 375, 404, 540 |
| TasI | AATT | 9 cut(s) | 11, 337, 481, 575, 587, 1115, 1233, 1254, 1321 |
| TauI | GCSGC | 3 cut(s) | 31, 70, 556 |
| TfiI | GAWTC | 6 cut(s) | 225, 411, 674, 913, 934, 1097 |
| Tru1I | TTAA | 9 cut(s) | 357, 456, 585, 1104, 1164, 1232, 1320, 1331, 1537 |
| Tru9I | TTAA | 9 cut(s) | 357, 456, 585, 1104, 1164, 1232, 1320, 1331, 1537 |
| TscAI | CASTG | 2 cut(s) | 1307, 1501 |
| TseFI | GTSAC | 3 cut(s) | 592, 970, 1225 |
| TseI | GCWGC | 5 cut(s) | 580, 1158, 1366, 1424, 1466 |
| Tsp45I | GTSAC | 3 cut(s) | 592, 970, 1225 |
| TspDTI | ATGAA | 5 cut(s) | 287, 588, 727, 734, 1384 |
| TspGWI | ACGGA | 1 cut(s) | 338 |
| TspMI | CCCGGG | 1 cut(s) | 160 |
| TspRI | CASTG | 2 cut(s) | 1307, 1501 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 1396 |
| VpaK11BI | GGWCC | 1 cut(s) | 325 |
| VspI | ATTAAT | 1 cut(s) | 456 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 1282 |
| XapI | RAATTY | 3 cut(s) | 337, 587, 1115 |
| XceI | RCATGY | 2 cut(s) | 190, 1158 |
| XmaI | CCCGGG | 1 cut(s) | 160 |
| XmaJI | CCTAGG | 1 cut(s) | 686 |
| XmiI | GTMKAC | 1 cut(s) | 636 |
| XmnI | GAANNNNTTC | 1 cut(s) | 278 |
| XspI | CTAG | 4 cut(s) | 210, 687, 1005, 1470 |
| ZraI | GACGTC | 1 cut(s) | 261 |