Prupe.7G234900_v2.0.a1

The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp07
OG0001510
Physical Location & Seq
Forward (+)
20441531 .. 20445251
3721 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.7G234900.2

Sequence Viewer

Length: 1509 bp
ATGCTTAAGCAAATTCTAAGCAAACTCCCCCGCAAGTCGCCAAAATCTGATTCATTAGATTCTGCAGGGAGTGATTCTGGCAGCAATACTCCCAATTTGGGCAATGGGTTCCAATGTACAATTGGTGGCAGTTCTCTTACAAGCAAATTAAATGTTGTCAAACGGGTGTCTTCAGCAGTTTTCCCATCAAGCATTGCAGCTGGAGCTGAGGCAGTAGAGCCCCATCTATCCTTCAAAGAGGTTTCAAATCCACAGAAGCATAATCTGTTTATCAGCAAGCTGAACCTTTGCTGCGAGTTCATTGATTCCAGTGATCCAGTTAAGCAAGATCTTAAACGTGAAACATTGATAGAACTTGTTGATTTTGTTTCTTCTGGATCTGCAAAGTTCAGTGAACCAGCGATTTCTGCCTTGTGTAAGATGTGTGCAATGAACCTGTTTAGAGTTTTCCCACCTAAATACCGATCTAATTTTACTGGGGGTGAAACAGACGATGAAGAACCATTGTTTGATCCTGCTTGGTCACATCTGCAAATTGTGTATGATCTTCTGGTTCGGTTTATCAGTTATAGTTCACTTGACATAAAGGTGGCGAAAAAGCATGTGGATCATTCTTTTATCGTAAAATTACTTGATCTCTTTGACTCTGAGGACCCAAGAGAAAGAGACTGTTTGAAAACCATTCTGCATAGGATTTATGGAAAATTCATGGTACACAGACCTTTTATAAGAAAAGCTGTCAGCAATATCATATATCGGTTTGTTTTCGAAACTGAAAGGCATAATGGAATTTCGGAGCTGTTGGAGATTTTTGGGAGTGTAATTAGCGGGTTTGCTTTGCCACTGAAGGAGGAACACAAGATATTTTTGTGGAGGGCTCTGATTCCTCTACACAAACCAAAGTCAGTGGGTATTTATCATCAGCAATTGACATATTGTGTTGTACAGTTTATAGATAAGGATCCGAAGTTGGCAAGTACTGTAATAAAAGGGTTGTTGAAGTACTGGCCAGTAACAAATAGCCAGAAGGAGTTGATGTTTTTGAGTGAGTTAGAAGAGGTTTTGGAAATGACTAGCATGGACGAGTTCCAAAAGGTCATGGTCCCACTGTTCCGGCGAATAAGATGCTGCCTCAATAGTTCCCATTACCAGGTGGCTGAACGATCTCACTTGCTGTGGAACAATGAACACATCCTTAACCTTATTGCGCATAACCGCCAGGTGATCTTGCCGCTTGTCTTCCCAGCGATTGAGCGAAACACCCAAAACCACTGGAATCAAGCAGTGTTGAACCTGACGCTGAACGTGAAGAAGATGTTTAGCGATATGGATGAGGAGCTGGTACTTGCCTGCCAATGTAAGTTGGAGGAGGAAGATATAAGGTCAAGCGCTGCAGCTGAGAAGCGCAGGTTAACATGGGAACGCTTGGAAACTGCTGCTGGTGTCCAACCCGTAGCTTGTAACTTTTTAGCTCCGATAAAGACTGCTGCTTGTCCTGTTGTCTGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03015 ko04071 ko04113 ko04114 ko04151 ko04152 ko04261 ko04728 ko05165 map03015 map04071 map04113 map04114 map04151 map04152 map04261 map04728 map05165
Pfam Domains
Protein Families
B56

Protein Analysis

503

Amino Acids

56.96

Weight (kDa)

7.88

Isoelectric Point (pI)

47.59

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 728
Acc16I TGCGCA 1 cut(s) 1209
Acc36I ACCTGC 1 cut(s) 1398
AciI CCGC 4 cut(s) 31, 828, 1216, 1232
AclWI GGATC 6 cut(s) 308, 385, 506, 615, 956, 969
AcoI YGGCCR 1 cut(s) 1007
AcsI RAATTY 3 cut(s) 12, 704, 789
AcuI CTGAAG 2 cut(s) 156, 866
AfaI GTAC 6 cut(s) 118, 714, 945, 979, 1004, 1344
AfeI AGCGCT 1 cut(s) 1390
AfiI CCNNNNNNNGG 2 cut(s) 98, 1150
AflII CTTAAG 1 cut(s) 5
AgsI TTSAA 5 cut(s) 235, 246, 676, 1000, 1291
AjnI CCWGG 2 cut(s) 1149, 1218
AjuI GAANNNNNNNTTGG 2 cut(s) 34, 66
AluBI AGCT 9 cut(s) 200, 206, 280, 737, 799, 1339, 1397, 1457, 1472
AluI AGCT 9 cut(s) 200, 206, 280, 737, 799, 1339, 1397, 1457, 1472
Alw26I GTCTC 1 cut(s) 660
AlwI GGATC 6 cut(s) 308, 385, 506, 615, 956, 969
Aor51HI AGCGCT 1 cut(s) 1390
AoxI GGCC 1 cut(s) 1007
ApeKI GCWGC 8 cut(s) 81, 197, 291, 1128, 1391, 1394, 1436, 1487
ApoI RAATTY 3 cut(s) 12, 704, 789
AspLEI GCGC 3 cut(s) 1210, 1391, 1407
AspS9I GGNCC 2 cut(s) 652, 1102
AsuHPI GGTGA 2 cut(s) 494, 1234
AsuII TTCGAA 1 cut(s) 768
AvaII GGWCC 2 cut(s) 652, 1102
BalI TGGCCA 1 cut(s) 1009
BamHI GGATCC 1 cut(s) 961
BanII GRGCYC 2 cut(s) 222, 880
BbsI GAAGAC 2 cut(s) 162, 1231
BbvCI CCTCAGC 1 cut(s) 207
BbvI GCAGC 8 cut(s) 93, 209, 278, 1115, 1378, 1406, 1423, 1474
BccI CCATC 2 cut(s) 193, 231
BcgI CGANNNNNNTGC 2 cut(s) 1107, 1141
BciT130I CCWGG 2 cut(s) 1151, 1220
BcoDI GTCTC 1 cut(s) 660
BfaI CTAG 1 cut(s) 1074
BfmI CTRYAG 2 cut(s) 63, 1392
BfoI RGCGCY 1 cut(s) 1392
BfrI CTTAAG 1 cut(s) 5
BfuAI ACCTGC 1 cut(s) 1398
BglII AGATCT 1 cut(s) 328
BisI GCNGC 9 cut(s) 82, 198, 292, 1129, 1232, 1392, 1395, 1437, 1488
BlsI GCNGC 9 cut(s) 83, 199, 293, 1130, 1233, 1393, 1396, 1438, 1489
BmcAI AGTACT 2 cut(s) 979, 1004
Bme1390I CCNGG 2 cut(s) 1151, 1220
Bme18I GGWCC 2 cut(s) 652, 1102
BmgT120I GGNCC 2 cut(s) 652, 1102
BmiI GGNNCC 4 cut(s) 110, 654, 963, 1104
BmrFI CCNGG 2 cut(s) 1151, 1220
BmrI ACTGGG 1 cut(s) 486
BmsI GCATC 1 cut(s) 1115
BmuI ACTGGG 1 cut(s) 486
BpiI GAAGAC 2 cut(s) 162, 1231
BpmI CTGGAG 1 cut(s) 222
Bpu10I CCTNAGC 1 cut(s) 207
Bpu14I TTCGAA 1 cut(s) 768
BsaBI GATNNNNATC 1 cut(s) 960
BsaXI ACNNNNNCTCC 2 cut(s) 1022, 1052
Bsc4I CCNNNNNNNGG 2 cut(s) 98, 1150
Bse1I ACTGG 6 cut(s) 309, 317, 481, 1010, 1010, 1277
Bse3DI GCAATG 3 cut(s) 109, 192, 435
Bse8I GATNNNNATC 1 cut(s) 960
BseBI CCWGG 2 cut(s) 1151, 1220
BseGI GGATG 2 cut(s) 1191, 1336
BseJI GATNNNNATC 1 cut(s) 960
BseLI CCNNNNNNNGG 2 cut(s) 98, 1150
BseMI GCAATG 3 cut(s) 109, 192, 435
BseMII CTCAG 3 cut(s) 198, 639, 1389
BseNI ACTGG 6 cut(s) 309, 317, 481, 1010, 1010, 1277
BseRI GAGGAG 2 cut(s) 1349, 1382
BseXI GCAGC 8 cut(s) 93, 209, 278, 1115, 1378, 1406, 1423, 1474
BseYI CCCAGC 1 cut(s) 1243
BshFI GGCC 1 cut(s) 1009
BsiSI CCGG 1 cut(s) 1114
BslFI GGGAC 1 cut(s) 1088
BslI CCNNNNNNNGG 2 cut(s) 98, 1150
BsmAI GTCTC 1 cut(s) 660
BsmFI GGGAC 1 cut(s) 1088
BsnI GGCC 1 cut(s) 1009
Bsp119I TTCGAA 1 cut(s) 768
Bsp1286I GDGCHC 2 cut(s) 222, 880
Bsp1407I TGTACA 2 cut(s) 116, 943
BspACI CCGC 4 cut(s) 31, 828, 1216, 1232
BspANI GGCC 1 cut(s) 1009
BspCNI CTCAG 3 cut(s) 199, 640, 1390
BspLI GGNNCC 4 cut(s) 110, 654, 963, 1104
BspMAI CTGCAG 2 cut(s) 67, 1396
BspMI ACCTGC 1 cut(s) 1398
BspPI GGATC 6 cut(s) 308, 385, 506, 615, 956, 969
BspT104I TTCGAA 1 cut(s) 768
BspTI CTTAAG 1 cut(s) 5
BsrDI GCAATG 3 cut(s) 109, 192, 435
BsrGI TGTACA 2 cut(s) 116, 943
BsrI ACTGG 6 cut(s) 309, 317, 481, 1010, 1010, 1277
Bst2UI CCWGG 2 cut(s) 1151, 1220
Bst4CI ACNGT 4 cut(s) 671, 948, 982, 1110
Bst6I CTCTTC 1 cut(s) 1050
BstAFI CTTAAG 1 cut(s) 5
BstAUI TGTACA 2 cut(s) 116, 943
BstBI TTCGAA 1 cut(s) 768
BstC8I GCNNGC 2 cut(s) 278, 1351
BstDEI CTNAG 4 cut(s) 17, 207, 648, 1398
BstF5I GGATG 2 cut(s) 1191, 1336
BstH2I RGCGCY 1 cut(s) 1392
BstHHI GCGC 3 cut(s) 1210, 1391, 1407
BstMAI GTCTC 1 cut(s) 660
BstMWI GCNNNNNNNGC 2 cut(s) 203, 407
BstNI CCWGG 2 cut(s) 1151, 1220
BstNSI RCATGY 1 cut(s) 605
BstSCI CCNGG 2 cut(s) 1149, 1218
BstSFI CTRYAG 2 cut(s) 63, 1392
BstV1I GCAGC 8 cut(s) 93, 209, 278, 1115, 1378, 1406, 1423, 1474
BstV2I GAAGAC 2 cut(s) 162, 1231
BstX2I RGATCY 3 cut(s) 328, 377, 961
BstYI RGATCY 3 cut(s) 328, 377, 961
BsuRI GGCC 1 cut(s) 1009
BtsCI GGATG 2 cut(s) 1191, 1336
BtsI GCAGTG 1 cut(s) 1290
BtsIMutI CAGTG 7 cut(s) 316, 397, 842, 912, 1106, 1270, 1290
BveI ACCTGC 1 cut(s) 1398
Cac8I GCNNGC 2 cut(s) 278, 1351
CfoI GCGC 3 cut(s) 1210, 1391, 1407
Cfr13I GGNCC 2 cut(s) 652, 1102
CseI GACGC 1 cut(s) 1306
CsiI ACCWGGT 1 cut(s) 1149
Csp6I GTAC 6 cut(s) 117, 713, 944, 978, 1003, 1343
CspCI CAANNNNNGTGG 2 cut(s) 888, 923
CviAII CATG 5 cut(s) 602, 709, 1078, 1099, 1416
CviQI GTAC 6 cut(s) 117, 713, 944, 978, 1003, 1343
DdeI CTNAG 4 cut(s) 17, 207, 648, 1398
EaeI YGGCCR 1 cut(s) 1007
Eam1104I CTCTTC 1 cut(s) 1050
EarI CTCTTC 1 cut(s) 1050
Eco24I GRGCYC 2 cut(s) 222, 880
Eco47I GGWCC 2 cut(s) 652, 1102
Eco47III AGCGCT 1 cut(s) 1390
Eco57I CTGAAG 2 cut(s) 156, 866
EcoO109I RGGNCCY 1 cut(s) 652
EcoRII CCWGG 2 cut(s) 1149, 1218
EcoT38I GRGCYC 2 cut(s) 222, 880
FaeI CATG 5 cut(s) 605, 712, 1081, 1102, 1419
FaqI GGGAC 1 cut(s) 1088
FatI CATG 5 cut(s) 601, 708, 1077, 1098, 1415
FauI CCCGC 2 cut(s) 38, 821
Fnu4HI GCNGC 9 cut(s) 82, 198, 292, 1129, 1232, 1392, 1395, 1437, 1488
FokI GGATG 2 cut(s) 1178, 1343
FriOI GRGCYC 2 cut(s) 222, 880
Fsp4HI GCNGC 9 cut(s) 82, 198, 292, 1129, 1232, 1392, 1395, 1437, 1488
FspBI CTAG 1 cut(s) 1074
FspI TGCGCA 1 cut(s) 1209
GlaI GCGC 3 cut(s) 1209, 1390, 1406
GluI GCNGC 9 cut(s) 82, 198, 292, 1129, 1232, 1392, 1395, 1437, 1488
GsaI CCCAGC 1 cut(s) 1247
GsuI CTGGAG 1 cut(s) 222
HaeII RGCGCY 1 cut(s) 1392
HaeIII GGCC 1 cut(s) 1009
HapII CCGG 1 cut(s) 1114
HgaI GACGC 1 cut(s) 1306
HhaI GCGC 3 cut(s) 1210, 1391, 1407
Hin1II CATG 5 cut(s) 605, 712, 1081, 1102, 1419
Hin6I GCGC 3 cut(s) 1208, 1389, 1405
HinP1I GCGC 3 cut(s) 1208, 1389, 1405
HincII GTYRAC 1 cut(s) 1413
HindII GTYRAC 1 cut(s) 1413
HinfI GANTC 7 cut(s) 50, 59, 74, 305, 644, 883, 1276
HpaI GTTAAC 1 cut(s) 1413
HpaII CCGG 1 cut(s) 1114
HphI GGTGA 2 cut(s) 494, 1234
Hpy166II GTNNAC 4 cut(s) 395, 575, 715, 1413
Hpy188I TCNGA 6 cut(s) 49, 649, 796, 882, 966, 1476
Hpy188III TCNNGA 1 cut(s) 375
Hpy8I GTNNAC 4 cut(s) 395, 575, 715, 1413
HpyAV CCTTC 3 cut(s) 241, 841, 1021
HpyCH4III ACNGT 4 cut(s) 671, 948, 982, 1110
HpyCH4IV ACGT 2 cut(s) 337, 1305
HpyCH4V TGCA 7 cut(s) 65, 197, 383, 428, 532, 688, 1394
HpyF10VI GCNNNNNNNGC 2 cut(s) 203, 407
HpyF3I CTNAG 4 cut(s) 17, 207, 648, 1398
HpySE526I ACGT 2 cut(s) 337, 1305
Hsp92II CATG 5 cut(s) 605, 712, 1081, 1102, 1419
HspAI GCGC 3 cut(s) 1208, 1389, 1405
KspAI GTTAAC 1 cut(s) 1413
LmnI GCTCC 4 cut(s) 203, 796, 1336, 1477
Lsp1109I GCAGC 8 cut(s) 93, 209, 278, 1115, 1378, 1406, 1423, 1474
LweI GCATC 1 cut(s) 1115
MabI ACCWGGT 1 cut(s) 1149
MaeI CTAG 1 cut(s) 1074
MaeII ACGT 2 cut(s) 337, 1305
MaeIII GTNAC 3 cut(s) 522, 1012, 1460
MboII GAAGA 9 cut(s) 162, 363, 509, 539, 1067, 1231, 1321, 1324, 1385
MfeI CAATTG 2 cut(s) 120, 926
MflI RGATCY 3 cut(s) 328, 377, 961
MhlI GDGCHC 2 cut(s) 222, 880
MlsI TGGCCA 1 cut(s) 1009
MluNI TGGCCA 1 cut(s) 1009
MlyI GAGTC 1 cut(s) 638
MmeI TCCRAC 3 cut(s) 783, 1344, 1471
Mox20I TGGCCA 1 cut(s) 1009
MscI TGGCCA 1 cut(s) 1009
MseI TTAA 6 cut(s) 6, 149, 321, 333, 1197, 1412
MslI CAYNNNNRTG 1 cut(s) 587
Msp20I TGGCCA 1 cut(s) 1009
MspA1I CMGCKG 2 cut(s) 200, 1397
MspCI CTTAAG 1 cut(s) 5
MspI CCGG 1 cut(s) 1114
MspR9I CCNGG 2 cut(s) 1151, 1220
MunI CAATTG 2 cut(s) 120, 926
MvaI CCWGG 2 cut(s) 1151, 1220
MwoI GCNNNNNNNGC 2 cut(s) 203, 407
NlaIII CATG 5 cut(s) 605, 712, 1081, 1102, 1419
NlaIV GGNNCC 4 cut(s) 110, 654, 963, 1104
NmuCI GTSAC 1 cut(s) 522
NsbI TGCGCA 1 cut(s) 1209
NspI RCATGY 1 cut(s) 605
NspV TTCGAA 1 cut(s) 768
PfeI GAWTC 6 cut(s) 50, 59, 74, 305, 883, 1276
PkrI GCNGC 9 cut(s) 83, 199, 293, 1130, 1233, 1393, 1396, 1438, 1489
PleI GAGTC 1 cut(s) 638
PpsI GAGTC 1 cut(s) 638
PpuMI RGGWCCY 1 cut(s) 652
PsiI TTATAA 1 cut(s) 728
Psp5II RGGWCCY 1 cut(s) 652
Psp6I CCWGG 2 cut(s) 1149, 1218
PspFI CCCAGC 1 cut(s) 1243
PspGI CCWGG 2 cut(s) 1149, 1218
PspN4I GGNNCC 4 cut(s) 110, 654, 963, 1104
PspPI GGNCC 2 cut(s) 652, 1102
PspPPI RGGWCCY 1 cut(s) 652
PstI CTGCAG 2 cut(s) 67, 1396
PsuI RGATCY 3 cut(s) 328, 377, 961
PvuII CAGCTG 2 cut(s) 200, 1397
RsaI GTAC 6 cut(s) 118, 714, 945, 979, 1004, 1344
RsaNI GTAC 6 cut(s) 117, 713, 944, 978, 1003, 1343
RseI CAYNNNNRTG 1 cut(s) 587
SaqAI TTAA 6 cut(s) 6, 149, 321, 333, 1197, 1412
SatI GCNGC 9 cut(s) 82, 198, 292, 1129, 1232, 1392, 1395, 1437, 1488
Sau96I GGNCC 2 cut(s) 652, 1102
ScaI AGTACT 2 cut(s) 979, 1004
SchI GAGTC 1 cut(s) 638
ScrFI CCNGG 2 cut(s) 1151, 1220
SduI GDGCHC 2 cut(s) 222, 880
SexAI ACCWGGT 1 cut(s) 1149
SfaNI GCATC 1 cut(s) 1115
SfcI CTRYAG 2 cut(s) 63, 1392
SfuI TTCGAA 1 cut(s) 768
SinI GGWCC 2 cut(s) 652, 1102
SmiMI CAYNNNNRTG 1 cut(s) 587
SmlI CTYRAG 1 cut(s) 5
SmoI CTYRAG 1 cut(s) 5
SsiI CCGC 4 cut(s) 31, 828, 1216, 1232
SspMI CTAG 1 cut(s) 1074
StyD4I CCNGG 2 cut(s) 1149, 1218
TaaI ACNGT 4 cut(s) 671, 948, 982, 1110
TaiI ACGT 2 cut(s) 340, 1308
TaqI TCGA 1 cut(s) 768
TatI WGTACW 4 cut(s) 116, 943, 977, 1002
TauI GCSGC 1 cut(s) 1234
TfiI GAWTC 6 cut(s) 50, 59, 74, 305, 883, 1276
Tru1I TTAA 6 cut(s) 6, 149, 321, 333, 1197, 1412
Tru9I TTAA 6 cut(s) 6, 149, 321, 333, 1197, 1412
TscAI CASTG 7 cut(s) 316, 397, 849, 912, 1113, 1277, 1290
TseFI GTSAC 1 cut(s) 522
TseI GCWGC 8 cut(s) 81, 197, 291, 1128, 1391, 1394, 1436, 1487
Tsp45I GTSAC 1 cut(s) 522
TspDTI ATGAA 6 cut(s) 42, 289, 446, 510, 697, 1200
TspRI CASTG 7 cut(s) 316, 397, 849, 912, 1113, 1277, 1290
Vha464I CTTAAG 1 cut(s) 5
VpaK11BI GGWCC 2 cut(s) 652, 1102
XapI RAATTY 3 cut(s) 12, 704, 789
XceI RCATGY 1 cut(s) 605
XcmI CCANNNNNNNNNTGG 1 cut(s) 119
XspI CTAG 1 cut(s) 1074
ZrmI AGTACT 2 cut(s) 979, 1004
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.