RLG00000016056

Aux IAA proteins are short-lived transcriptional factors that function as repressors of early auxin response genes at low auxin concentrations

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0001510
Physical Location & Seq
Reverse (-)
3526143 .. 3527136
994 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000016056

Sequence Viewer

Length: 693 bp
ATGAATTACAAGGACACTGAGCTGACCTTGGGATTACCTGGTGGCGGAAGCCGTGGTTGTTCAACAGGTGTAGTGGTCTTCGGAGAGTTGATGAAGAGCTCGATGAAACGTGGGTTCATGTATACCGATGATGATCTCAACAACCTTGACCTGGAAAGCTCTAATGGCGAGCCTCGTGGCCAAGCTAAATTGGATGATGGGCCGTCGATGGAAACAGCTAAGTACTCTTCCAGTAGTACCAGTACAACTGCCGTGAAAGCCCCAACTGCAGAGGGTCAAGTAGTTGGGTGGCCGCCGGTGAGATCGACGAGAAATAAGGCCTTGGACTCGAAGAACTTGAAGAACAACAGCAGAAGTAAATATGTGAAAGTGGGTGCCGATGGAGCTCCATACCTGCGGAAAGTAGATTTAGAGATGTATAAAAGTTACGAGGAGCTATTAACAGCCTTGGAGAAGATTTTCACTTCCTTCACCATAAACGGCAAGTACATGGAGGATGATAGGAAGCAACAGGTGCTTTTTATGGAACATGCAGTAGTGAATGGAACAGTGGAATATAATCAATATGTGGTGACATATGAAGACAAGGATAATGACTGGATGTTAGTTGGAGACGTTCCTTGGAAAATGTTTAAAGATTCGTGCAAGCGTATCCGGTTGATGAAATGTTCAGAAGCTGCGGTCAGAAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0009719 GO:0009725 GO:0009733 GO:0010033 GO:0042221 GO:0050896
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
AUX_IAA

Protein Analysis

231

Amino Acids

25.75

Weight (kDa)

6.63

Isoelectric Point (pI)

35.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AUX_IAA PF02309 5 - 227 4.2e-57 AUX/IAA family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 402
AccB1I GGYRCC 1 cut(s) 374
AccI GTMKAC 1 cut(s) 122
AciI CCGC 4 cut(s) 45, 293, 397, 680
AcoI YGGCCR 2 cut(s) 178, 290
AfaI GTAC 4 cut(s) 224, 238, 244, 488
AfiI CCNNNNNNNGG 2 cut(s) 44, 151
AgsI TTSAA 2 cut(s) 63, 340
AjnI CCWGG 2 cut(s) 37, 150
AluBI AGCT 8 cut(s) 22, 99, 159, 185, 218, 386, 436, 677
AluI AGCT 8 cut(s) 22, 99, 159, 185, 218, 386, 436, 677
Alw21I GWGCWC 2 cut(s) 101, 388
Alw26I GTCTC 1 cut(s) 606
AlwNI CAGNNNCTG 1 cut(s) 677
AoxI GGCC 4 cut(s) 178, 200, 290, 318
ApeKI GCWGC 1 cut(s) 677
Asp700I GAANNNNTTC 1 cut(s) 458
AspS9I GGNCC 1 cut(s) 200
AsuHPI GGTGA 3 cut(s) 310, 463, 583
BalI TGGCCA 1 cut(s) 180
BanI GGYRCC 1 cut(s) 374
BanII GRGCYC 2 cut(s) 101, 388
BauI CACGAG 1 cut(s) 174
BbsI GAAGAC 2 cut(s) 70, 588
Bbv12I GWGCWC 2 cut(s) 101, 388
BbvI GCAGC 1 cut(s) 664
BccI CCATC 3 cut(s) 191, 202, 374
BceAI ACGGC 4 cut(s) 36, 187, 236, 496
BciT130I CCWGG 2 cut(s) 39, 152
BciVI GTATCC 1 cut(s) 662
BcoDI GTCTC 1 cut(s) 606
BfmI CTRYAG 1 cut(s) 267
BfuAI ACCTGC 1 cut(s) 402
BfuI GTATCC 1 cut(s) 662
BisI GCNGC 2 cut(s) 293, 678
BlsI GCNGC 2 cut(s) 294, 679
BmcAI AGTACT 1 cut(s) 224
Bme1390I CCNGG 2 cut(s) 39, 152
BmgT120I GGNCC 1 cut(s) 200
BmiI GGNNCC 1 cut(s) 376
BmrFI CCNGG 2 cut(s) 39, 152
BpiI GAAGAC 2 cut(s) 70, 588
BsaBI GATNNNNATC 1 cut(s) 132
BsaJI CCNNGG 5 cut(s) 27, 52, 321, 447, 620
BsaWI WCCGGW 1 cut(s) 654
Bsc4I CCNNNNNNNGG 2 cut(s) 44, 151
Bse118I RCCGGY 1 cut(s) 295
Bse1I ACTGG 3 cut(s) 231, 240, 602
Bse8I GATNNNNATC 1 cut(s) 132
BseBI CCWGG 2 cut(s) 39, 152
BseDI CCNNGG 5 cut(s) 27, 52, 321, 447, 620
BseGI GGATG 3 cut(s) 199, 502, 606
BseJI GATNNNNATC 1 cut(s) 132
BseLI CCNNNNNNNGG 2 cut(s) 44, 151
BseMII CTCAG 1 cut(s) 9
BseNI ACTGG 3 cut(s) 231, 240, 602
BseRI GAGGAG 1 cut(s) 446
BseXI GCAGC 1 cut(s) 664
BshFI GGCC 4 cut(s) 180, 202, 292, 320
BshNI GGYRCC 1 cut(s) 374
BsiHKAI GWGCWC 2 cut(s) 101, 388
BsiSI CCGG 2 cut(s) 296, 655
BslI CCNNNNNNNGG 2 cut(s) 44, 151
BsmAI GTCTC 1 cut(s) 606
BsmBI CGTCTC 1 cut(s) 606
BsnI GGCC 4 cut(s) 180, 202, 292, 320
Bsp1286I GDGCHC 2 cut(s) 101, 388
Bsp143I GATC 2 cut(s) 133, 302
BspACI CCGC 4 cut(s) 45, 293, 397, 680
BspANI GGCC 4 cut(s) 180, 202, 292, 320
BspCNI CTCAG 1 cut(s) 10
BspLI GGNNCC 1 cut(s) 376
BspMAI CTGCAG 1 cut(s) 271
BspMI ACCTGC 1 cut(s) 402
BspQI GCTCTTC 1 cut(s) 89
BspT107I GGYRCC 1 cut(s) 374
BsrFI RCCGGY 1 cut(s) 295
BsrI ACTGG 3 cut(s) 231, 240, 602
BssAI RCCGGY 1 cut(s) 295
BssECI CCNNGG 5 cut(s) 27, 52, 321, 447, 620
BssMI GATC 2 cut(s) 133, 302
BssNAI GTATAC 1 cut(s) 123
BssSI CACGAG 1 cut(s) 174
BssT1I CCWWGG 4 cut(s) 27, 321, 447, 620
Bst1107I GTATAC 1 cut(s) 123
Bst2BI CACGAG 1 cut(s) 174
Bst2UI CCWGG 2 cut(s) 39, 152
Bst4CI ACNGT 1 cut(s) 550
Bst6I CTCTTC 2 cut(s) 89, 232
BstAPI GCANNNNNTGC 1 cut(s) 514
BstC8I GCNNGC 2 cut(s) 170, 647
BstDEI CTNAG 2 cut(s) 18, 219
BstDSI CCRYGG 1 cut(s) 52
BstF5I GGATG 3 cut(s) 199, 502, 606
BstKTI GATC 2 cut(s) 136, 305
BstMAI GTCTC 1 cut(s) 606
BstMBI GATC 2 cut(s) 133, 302
BstMWI GCNNNNNNNGC 5 cut(s) 165, 257, 266, 383, 514
BstNI CCWGG 2 cut(s) 39, 152
BstNSI RCATGY 1 cut(s) 533
BstSCI CCNGG 2 cut(s) 37, 150
BstSFI CTRYAG 1 cut(s) 267
BstV1I GCAGC 1 cut(s) 664
BstV2I GAAGAC 2 cut(s) 70, 588
BstZ17I GTATAC 1 cut(s) 123
BsuI GTATCC 1 cut(s) 662
BsuRI GGCC 4 cut(s) 180, 202, 292, 320
BtgI CCRYGG 1 cut(s) 52
BtsCI GGATG 3 cut(s) 199, 502, 606
BtsIMutI CAGTG 2 cut(s) 15, 555
BveI ACCTGC 1 cut(s) 402
Cac8I GCNNGC 2 cut(s) 170, 647
CaiI CAGNNNCTG 1 cut(s) 677
Cfr10I RCCGGY 1 cut(s) 295
Cfr13I GGNCC 1 cut(s) 200
CsiI ACCWGGT 1 cut(s) 37
Csp6I GTAC 4 cut(s) 223, 237, 243, 487
CviAII CATG 3 cut(s) 118, 490, 530
CviQI GTAC 4 cut(s) 223, 237, 243, 487
DdeI CTNAG 2 cut(s) 18, 219
DpnI GATC 2 cut(s) 135, 304
DpnII GATC 2 cut(s) 133, 302
DraI TTTAAA 1 cut(s) 634
EaeI YGGCCR 2 cut(s) 178, 290
Eam1104I CTCTTC 2 cut(s) 89, 232
EarI CTCTTC 2 cut(s) 89, 232
EciI GGCGGA 1 cut(s) 60
Ecl136II GAGCTC 2 cut(s) 99, 386
Eco130I CCWWGG 4 cut(s) 27, 321, 447, 620
Eco147I AGGCCT 1 cut(s) 320
Eco24I GRGCYC 2 cut(s) 101, 388
Eco53kI GAGCTC 2 cut(s) 99, 386
EcoICRI GAGCTC 2 cut(s) 99, 386
EcoRII CCWGG 2 cut(s) 37, 150
EcoT14I CCWWGG 4 cut(s) 27, 321, 447, 620
EcoT38I GRGCYC 2 cut(s) 101, 388
ErhI CCWWGG 4 cut(s) 27, 321, 447, 620
Esp3I CGTCTC 1 cut(s) 606
FaeI CATG 3 cut(s) 121, 493, 533
FatI CATG 3 cut(s) 117, 489, 529
FauNDI CATATG 1 cut(s) 577
FblI GTMKAC 1 cut(s) 122
Fnu4HI GCNGC 2 cut(s) 293, 678
FokI GGATG 3 cut(s) 206, 509, 613
FriOI GRGCYC 2 cut(s) 101, 388
Fsp4HI GCNGC 2 cut(s) 293, 678
GluI GCNGC 2 cut(s) 293, 678
HaeIII GGCC 4 cut(s) 180, 202, 292, 320
HapII CCGG 2 cut(s) 296, 655
Hin1II CATG 3 cut(s) 121, 493, 533
HinfI GANTC 2 cut(s) 326, 638
HpaII CCGG 2 cut(s) 296, 655
HphI GGTGA 3 cut(s) 310, 463, 583
Hpy166II GTNNAC 1 cut(s) 123
Hpy188I TCNGA 3 cut(s) 83, 673, 686
Hpy8I GTNNAC 1 cut(s) 123
Hpy99I CGWCG 2 cut(s) 208, 310
HpyAV CCTTC 1 cut(s) 478
HpyCH4III ACNGT 1 cut(s) 550
HpyCH4IV ACGT 2 cut(s) 109, 615
HpyCH4V TGCA 3 cut(s) 269, 533, 645
HpyF10VI GCNNNNNNNGC 5 cut(s) 165, 257, 266, 383, 514
HpyF3I CTNAG 2 cut(s) 18, 219
HpySE526I ACGT 2 cut(s) 109, 615
Hsp92II CATG 3 cut(s) 121, 493, 533
Kzo9I GATC 2 cut(s) 133, 302
LguI GCTCTTC 1 cut(s) 89
LmnI GCTCC 3 cut(s) 383, 391, 433
Lsp1109I GCAGC 1 cut(s) 664
MabI ACCWGGT 1 cut(s) 37
MaeII ACGT 2 cut(s) 109, 615
MaeIII GTNAC 2 cut(s) 425, 571
MalI GATC 2 cut(s) 135, 304
MboI GATC 2 cut(s) 133, 302
MboII GAAGA 7 cut(s) 70, 106, 219, 343, 352, 466, 593
MhlI GDGCHC 2 cut(s) 101, 388
MlsI TGGCCA 1 cut(s) 180
MluCI AATT 3 cut(s) 4, 188, 688
MluNI TGGCCA 1 cut(s) 180
MlyI GAGTC 1 cut(s) 320
MmeI TCCRAC 1 cut(s) 589
MnlI CCTC 4 cut(s) 183, 265, 424, 487
Mox20I TGGCCA 1 cut(s) 180
MroXI GAANNNNTTC 1 cut(s) 458
MscI TGGCCA 1 cut(s) 180
MseI TTAA 2 cut(s) 440, 633
Msp20I TGGCCA 1 cut(s) 180
MspI CCGG 2 cut(s) 296, 655
MspR9I CCNGG 2 cut(s) 39, 152
MvaI CCWGG 2 cut(s) 39, 152
MwoI GCNNNNNNNGC 5 cut(s) 165, 257, 266, 383, 514
NdeI CATATG 1 cut(s) 577
NdeII GATC 2 cut(s) 133, 302
NlaIII CATG 3 cut(s) 121, 493, 533
NlaIV GGNNCC 1 cut(s) 376
NmuCI GTSAC 1 cut(s) 571
NspI RCATGY 1 cut(s) 533
PceI AGGCCT 1 cut(s) 320
PciSI GCTCTTC 1 cut(s) 89
PdmI GAANNNNTTC 1 cut(s) 458
PfeI GAWTC 1 cut(s) 638
PkrI GCNGC 2 cut(s) 294, 679
PleI GAGTC 1 cut(s) 320
PpsI GAGTC 1 cut(s) 320
Psp124BI GAGCTC 2 cut(s) 101, 388
Psp6I CCWGG 2 cut(s) 37, 150
PspGI CCWGG 2 cut(s) 37, 150
PspN4I GGNNCC 1 cut(s) 376
PspPI GGNCC 1 cut(s) 200
PstI CTGCAG 1 cut(s) 271
PstNI CAGNNNCTG 1 cut(s) 677
RsaI GTAC 4 cut(s) 224, 238, 244, 488
RsaNI GTAC 4 cut(s) 223, 237, 243, 487
SacI GAGCTC 2 cut(s) 101, 388
SapI GCTCTTC 1 cut(s) 89
SaqAI TTAA 2 cut(s) 440, 633
SatI GCNGC 2 cut(s) 293, 678
Sau3AI GATC 2 cut(s) 133, 302
Sau96I GGNCC 1 cut(s) 200
ScaI AGTACT 1 cut(s) 224
SchI GAGTC 1 cut(s) 320
ScrFI CCNGG 2 cut(s) 39, 152
SduI GDGCHC 2 cut(s) 101, 388
SexAI ACCWGGT 1 cut(s) 37
SfcI CTRYAG 1 cut(s) 267
SgrAI CRCCGGYG 1 cut(s) 295
Sse9I AATT 3 cut(s) 4, 188, 688
SseBI AGGCCT 1 cut(s) 320
SsiI CCGC 4 cut(s) 45, 293, 397, 680
SstI GAGCTC 2 cut(s) 101, 388
StuI AGGCCT 1 cut(s) 320
StyD4I CCNGG 2 cut(s) 37, 150
StyI CCWWGG 4 cut(s) 27, 321, 447, 620
TaaI ACNGT 1 cut(s) 550
TaiI ACGT 2 cut(s) 112, 618
TaqI TCGA 4 cut(s) 101, 206, 305, 329
TasI AATT 3 cut(s) 4, 188, 688
TatI WGTACW 3 cut(s) 222, 242, 486
TauI GCSGC 1 cut(s) 295
TfiI GAWTC 1 cut(s) 638
Tru1I TTAA 2 cut(s) 440, 633
Tru9I TTAA 2 cut(s) 440, 633
TscAI CASTG 2 cut(s) 22, 555
TseFI GTSAC 1 cut(s) 571
TseI GCWGC 1 cut(s) 677
Tsp45I GTSAC 1 cut(s) 571
TspDTI ATGAA 6 cut(s) 17, 106, 107, 119, 594, 677
TspRI CASTG 2 cut(s) 22, 555
XceI RCATGY 1 cut(s) 533
XmiI GTMKAC 1 cut(s) 122
XmnI GAANNNNTTC 1 cut(s) 458
ZrmI AGTACT 1 cut(s) 224
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.