FvH4_1g24040

VQ motif

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0003162
Physical Location & Seq
Reverse (-)
15898227 .. 15899990
1764 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g24040.t1

Sequence Viewer

Length: 846 bp
ATGTTAAGAAGACAAGTCAACCCCTCTTCCATGGTCAATACCTGTTGGCGTCAAACTCTTCCCTACATCTCTCTATATGTCATTTCCTCTCCTAAGCCAATTCTCTCCCATACCATTAACACAACCCACCACCAGATCTCCTCCTCCTCCGCCGCAATGAACCCACCTCCGTCGCTGCCCGCCACCCCCAAAAAGGAGCTCCAACTCCAAGGCCCCCGCCCCGCCCCTCTCAAACTCAGCAAAGACTCCGTCAAAATCAAGAAGCCACCGCCTCACCCCCACCTCCTCCGCCACAACCCACCTCCCCAACACCACTCCCTTCCGCCGCCGCAGCACCACCAACCCATCGTCATCTACACCGTCTCTCCCAAAGTCATCCACGCCTCCGTCAACGAGTTCATGTCCCTCGTCCAACGACTCACCGGCCCTTCCTCCTCTCCCCCCGGCAACCTCTCCCCCGCCGCCCGCCTCGCCTCCATCGAGAAAACCTCCCCCTCCTCCGATCACAATAAGCACTTCCCGCCTCTAAATACAACCCAAACCGCCGCCGCCATTGACGACGACTTTATGGACAGTATTCTGGAAGGTATTGACATCATGGGTCAGTTCCCGGGAATACTCTCCCCTGCGCCGGCGACCATGCCGCCGATCCCACCTGGGTACTTCTCACCGGCCGCTGAAGACCCGCAAAACTTCAACAACTGGCAAGGAGTAGTTGCGAGCTCCAACAACATCAACAGCGGCTTCAATAGCTTCATGGCCAGCCCTTCAACTCTGTTTTCTGCTCCTCTGGTTTCTCCGAGCCCTTCTTCATGGGACCTCTTCAACAATTTCTTAGACTCGTAG

Protein Analysis

282

Amino Acids

30.48

Weight (kDa)

9.04

Isoelectric Point (pI)

72.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VQ PF05678 120 - 147 3.5e-12 VQ motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 643
AcoI YGGCCR 2 cut(s) 672, 759
AcuI CTGAAG 1 cut(s) 699
AcyI GRCGYC 1 cut(s) 49
AfaI GTAC 1 cut(s) 662
AfiI CCNNNNNNNGG 2 cut(s) 193, 631
AgsI TTSAA 4 cut(s) 697, 748, 771, 826
AjnI CCWGG 1 cut(s) 655
AluBI AGCT 3 cut(s) 199, 723, 753
AluI AGCT 3 cut(s) 199, 723, 753
Alw21I GWGCWC 2 cut(s) 201, 725
Alw26I GTCTC 1 cut(s) 367
AlwI GGATC 1 cut(s) 643
Ama87I CYCGRG 1 cut(s) 610
AoxI GGCC 4 cut(s) 211, 424, 672, 759
ApeKI GCWGC 2 cut(s) 175, 331
ArsI GACNNNNNNTTYG 2 cut(s) 234, 266
AspLEI GCGC 1 cut(s) 631
AspS9I GGNCC 3 cut(s) 212, 425, 817
AsuC2I CCSGG 3 cut(s) 444, 611, 612
AsuHPI GGTGA 3 cut(s) 266, 412, 660
AvaI CYCGRG 1 cut(s) 610
AvaII GGWCC 1 cut(s) 817
BalI TGGCCA 1 cut(s) 761
BanII GRGCYC 3 cut(s) 201, 725, 806
BbsI GAAGAC 2 cut(s) 16, 687
Bbv12I GWGCWC 2 cut(s) 201, 725
BbvI GCAGC 2 cut(s) 162, 343
BccI CCATC 2 cut(s) 353, 485
BciT130I CCWGG 1 cut(s) 657
BcnI CCSGG 3 cut(s) 444, 611, 612
BcoDI GTCTC 1 cut(s) 367
BglII AGATCT 1 cut(s) 135
Bme1390I CCNGG 4 cut(s) 444, 611, 612, 657
Bme18I GGWCC 1 cut(s) 817
BmeT110I CYCGRG 1 cut(s) 610
BmgT120I GGNCC 3 cut(s) 212, 425, 817
BmiI GGNNCC 2 cut(s) 214, 818
BmrFI CCNGG 4 cut(s) 444, 611, 612, 657
BpiI GAAGAC 2 cut(s) 16, 687
BplI GAGNNNNNCTC 2 cut(s) 473, 505
Bpu10I CCTNAGC 1 cut(s) 93
BpuMI CCSGG 3 cut(s) 444, 611, 612
BsaHI GRCGYC 1 cut(s) 49
BsaJI CCNNGG 5 cut(s) 30, 208, 442, 610, 656
BsaXI ACNNNNNCTCC 4 cut(s) 122, 152, 349, 379
Bsc4I CCNNNNNNNGG 2 cut(s) 193, 631
Bse118I RCCGGY 3 cut(s) 422, 631, 670
Bse1I ACTGG 1 cut(s) 707
Bse3DI GCAATG 1 cut(s) 162
BseBI CCWGG 1 cut(s) 657
BseDI CCNNGG 5 cut(s) 30, 208, 442, 610, 656
BseGI GGATG 1 cut(s) 375
BseLI CCNNNNNNNGG 2 cut(s) 193, 631
BseMI GCAATG 1 cut(s) 162
BseMII CTCAG 1 cut(s) 250
BseNI ACTGG 1 cut(s) 707
BseRI GAGGAG 7 cut(s) 130, 133, 136, 275, 424, 487, 777
BseX3I CGGCCG 1 cut(s) 672
BseXI GCAGC 2 cut(s) 162, 343
Bsh1285I CGRYCG 1 cut(s) 675
BshFI GGCC 4 cut(s) 213, 426, 674, 761
BsiEI CGRYCG 1 cut(s) 675
BsiHKAI GWGCWC 2 cut(s) 201, 725
BsiHKCI CYCGRG 1 cut(s) 610
BsiSI CCGG 5 cut(s) 423, 444, 611, 632, 671
BslFI GGGAC 2 cut(s) 388, 830
BslI CCNNNNNNNGG 2 cut(s) 193, 631
BsmAI GTCTC 1 cut(s) 367
BsmBI CGTCTC 1 cut(s) 367
BsmFI GGGAC 2 cut(s) 388, 830
BsnI GGCC 4 cut(s) 213, 426, 674, 761
BsoBI CYCGRG 1 cut(s) 610
Bsp1286I GDGCHC 3 cut(s) 201, 725, 806
Bsp143I GATC 3 cut(s) 135, 502, 648
Bsp19I CCATGG 1 cut(s) 30
BspANI GGCC 4 cut(s) 213, 426, 674, 761
BspCNI CTCAG 1 cut(s) 249
BspLI GGNNCC 2 cut(s) 214, 818
BspPI GGATC 1 cut(s) 643
BsrDI GCAATG 1 cut(s) 162
BsrFI RCCGGY 3 cut(s) 422, 631, 670
BsrI ACTGG 1 cut(s) 707
BssAI RCCGGY 3 cut(s) 422, 631, 670
BssECI CCNNGG 5 cut(s) 30, 208, 442, 610, 656
BssMI GATC 3 cut(s) 135, 502, 648
BssNI GRCGYC 1 cut(s) 49
BssT1I CCWWGG 2 cut(s) 30, 208
Bst2UI CCWGG 1 cut(s) 657
Bst4CI ACNGT 2 cut(s) 361, 575
Bst6I CTCTTC 3 cut(s) 31, 63, 827
BstACI GRCGYC 1 cut(s) 49
BstC8I GCNNGC 5 cut(s) 180, 466, 633, 721, 763
BstDEI CTNAG 3 cut(s) 93, 236, 835
BstDSI CCRYGG 1 cut(s) 30
BstF5I GGATG 1 cut(s) 375
BstHHI GCGC 1 cut(s) 631
BstKTI GATC 3 cut(s) 138, 505, 651
BstMAI GTCTC 1 cut(s) 367
BstMBI GATC 3 cut(s) 135, 502, 648
BstMCI CGRYCG 1 cut(s) 675
BstMWI GCNNNNNNNGC 4 cut(s) 331, 470, 520, 750
BstNI CCWGG 1 cut(s) 657
BstSCI CCNGG 4 cut(s) 442, 609, 610, 655
BstV1I GCAGC 2 cut(s) 162, 343
BstV2I GAAGAC 2 cut(s) 16, 687
BstX2I RGATCY 1 cut(s) 135
BstYI RGATCY 1 cut(s) 135
BstZI CGGCCG 1 cut(s) 672
BsuRI GGCC 4 cut(s) 213, 426, 674, 761
BtgI CCRYGG 1 cut(s) 30
BtsCI GGATG 1 cut(s) 375
Cac8I GCNNGC 5 cut(s) 180, 466, 633, 721, 763
CfoI GCGC 1 cut(s) 631
Cfr10I RCCGGY 3 cut(s) 422, 631, 670
Cfr13I GGNCC 3 cut(s) 212, 425, 817
Cfr9I CCCGGG 1 cut(s) 610
CseI GACGC 1 cut(s) 38
Csp6I GTAC 1 cut(s) 661
CviAII CATG 6 cut(s) 31, 400, 598, 640, 757, 813
CviQI GTAC 1 cut(s) 661
DdeI CTNAG 3 cut(s) 93, 236, 835
DpnI GATC 3 cut(s) 137, 504, 650
DpnII GATC 3 cut(s) 135, 502, 648
EaeI YGGCCR 2 cut(s) 672, 759
EagI CGGCCG 1 cut(s) 672
Eam1104I CTCTTC 3 cut(s) 31, 63, 827
EarI CTCTTC 3 cut(s) 31, 63, 827
EciI GGCGGA 3 cut(s) 139, 278, 312
Ecl136II GAGCTC 2 cut(s) 199, 723
EclXI CGGCCG 1 cut(s) 672
Eco130I CCWWGG 2 cut(s) 30, 208
Eco24I GRGCYC 3 cut(s) 201, 725, 806
Eco47I GGWCC 1 cut(s) 817
Eco52I CGGCCG 1 cut(s) 672
Eco53kI GAGCTC 2 cut(s) 199, 723
Eco57I CTGAAG 1 cut(s) 699
Eco88I CYCGRG 1 cut(s) 610
EcoICRI GAGCTC 2 cut(s) 199, 723
EcoO109I RGGNCCY 2 cut(s) 212, 817
EcoRII CCWGG 1 cut(s) 655
EcoT14I CCWWGG 2 cut(s) 30, 208
EcoT38I GRGCYC 3 cut(s) 201, 725, 806
ErhI CCWWGG 2 cut(s) 30, 208
Esp3I CGTCTC 1 cut(s) 367
FaeI CATG 6 cut(s) 34, 403, 601, 643, 760, 816
FaqI GGGAC 2 cut(s) 388, 830
FatI CATG 6 cut(s) 30, 399, 597, 639, 756, 812
FauI CCCGC 7 cut(s) 187, 224, 229, 466, 473, 528, 693
FokI GGATG 1 cut(s) 362
FriOI GRGCYC 3 cut(s) 201, 725, 806
GlaI GCGC 1 cut(s) 630
HaeIII GGCC 4 cut(s) 213, 426, 674, 761
HapII CCGG 5 cut(s) 423, 444, 611, 632, 671
HgaI GACGC 1 cut(s) 38
HhaI GCGC 1 cut(s) 631
Hin1I GRCGYC 1 cut(s) 49
Hin1II CATG 6 cut(s) 34, 403, 601, 643, 760, 816
Hin6I GCGC 1 cut(s) 629
HinP1I GCGC 1 cut(s) 629
HincII GTYRAC 2 cut(s) 19, 391
HindII GTYRAC 2 cut(s) 19, 391
HinfI GANTC 3 cut(s) 245, 417, 839
HpaII CCGG 5 cut(s) 423, 444, 611, 632, 671
HphI GGTGA 3 cut(s) 266, 412, 660
Hpy166II GTNNAC 2 cut(s) 19, 391
Hpy188I TCNGA 2 cut(s) 502, 801
Hpy188III TCNNGA 3 cut(s) 259, 481, 581
Hpy8I GTNNAC 2 cut(s) 19, 391
Hpy99I CGWCG 2 cut(s) 175, 563
HpyAV CCTTC 5 cut(s) 329, 438, 578, 777, 816
HpyCH4III ACNGT 2 cut(s) 361, 575
HpyF10VI GCNNNNNNNGC 4 cut(s) 331, 470, 520, 750
HpyF3I CTNAG 3 cut(s) 93, 236, 835
Hsp92I GRCGYC 1 cut(s) 49
Hsp92II CATG 6 cut(s) 34, 403, 601, 643, 760, 816
HspAI GCGC 1 cut(s) 629
KroI GCCGGC 1 cut(s) 631
KroNI GCCGGC 1 cut(s) 633
Kzo9I GATC 3 cut(s) 135, 502, 648
LmnI GCTCC 4 cut(s) 196, 204, 728, 790
Lsp1109I GCAGC 2 cut(s) 162, 343
MalI GATC 3 cut(s) 137, 504, 650
MboI GATC 3 cut(s) 135, 502, 648
MboII GAAGA 6 cut(s) 18, 21, 50, 692, 801, 814
MflI RGATCY 1 cut(s) 135
MhlI GDGCHC 3 cut(s) 201, 725, 806
MlsI TGGCCA 1 cut(s) 761
MluCI AATT 2 cut(s) 99, 829
MluNI TGGCCA 1 cut(s) 761
MlyI GAGTC 3 cut(s) 239, 411, 833
MmeI TCCRAC 3 cut(s) 226, 436, 750
Mox20I TGGCCA 1 cut(s) 761
MreI CGCCGGCG 1 cut(s) 631
MroNI GCCGGC 1 cut(s) 631
MscI TGGCCA 1 cut(s) 761
MseI TTAA 2 cut(s) 5, 117
Msp20I TGGCCA 1 cut(s) 761
MspA1I CMGCKG 2 cut(s) 677, 741
MspI CCGG 5 cut(s) 423, 444, 611, 632, 671
MspR9I CCNGG 4 cut(s) 444, 611, 612, 657
MvaI CCWGG 1 cut(s) 657
MwoI GCNNNNNNNGC 4 cut(s) 331, 470, 520, 750
NaeI GCCGGC 1 cut(s) 633
NciI CCSGG 3 cut(s) 444, 611, 612
NcoI CCATGG 1 cut(s) 30
NdeII GATC 3 cut(s) 135, 502, 648
NgoMIV GCCGGC 1 cut(s) 631
NlaIII CATG 6 cut(s) 34, 403, 601, 643, 760, 816
NlaIV GGNNCC 2 cut(s) 214, 818
PcsI WCGNNNNNNNCGW 1 cut(s) 477
PdiI GCCGGC 1 cut(s) 633
PflFI GACNNNGTC 1 cut(s) 248
PleI GAGTC 3 cut(s) 239, 411, 833
PpsI GAGTC 3 cut(s) 239, 411, 833
PpuMI RGGWCCY 1 cut(s) 817
Psp124BI GAGCTC 2 cut(s) 201, 725
Psp5II RGGWCCY 1 cut(s) 817
Psp6I CCWGG 1 cut(s) 655
PspGI CCWGG 1 cut(s) 655
PspN4I GGNNCC 2 cut(s) 214, 818
PspPI GGNCC 3 cut(s) 212, 425, 817
PspPPI RGGWCCY 1 cut(s) 817
PsuI RGATCY 1 cut(s) 135
PsyI GACNNNGTC 1 cut(s) 248
RsaI GTAC 1 cut(s) 662
RsaNI GTAC 1 cut(s) 661
SacI GAGCTC 2 cut(s) 201, 725
SaqAI TTAA 2 cut(s) 5, 117
Sau3AI GATC 3 cut(s) 135, 502, 648
Sau96I GGNCC 3 cut(s) 212, 425, 817
SchI GAGTC 3 cut(s) 239, 411, 833
ScrFI CCNGG 4 cut(s) 444, 611, 612, 657
SduI GDGCHC 3 cut(s) 201, 725, 806
SgrAI CRCCGGYG 1 cut(s) 631
SinI GGWCC 1 cut(s) 817
SmaI CCCGGG 1 cut(s) 612
Sse9I AATT 2 cut(s) 99, 829
SstI GAGCTC 2 cut(s) 201, 725
StyD4I CCNGG 4 cut(s) 442, 609, 610, 655
StyI CCWWGG 2 cut(s) 30, 208
TaaI ACNGT 2 cut(s) 361, 575
TaqI TCGA 1 cut(s) 480
TasI AATT 2 cut(s) 99, 829
TauI GCSGC 9 cut(s) 155, 328, 331, 464, 548, 551, 646, 677, 744
Tru1I TTAA 2 cut(s) 5, 117
Tru9I TTAA 2 cut(s) 5, 117
TseI GCWGC 2 cut(s) 175, 331
TspDTI ATGAA 4 cut(s) 173, 388, 745, 801
TspGWI ACGGA 3 cut(s) 159, 238, 376
TspMI CCCGGG 1 cut(s) 610
Tth111I GACNNNGTC 1 cut(s) 248
VpaK11BI GGWCC 1 cut(s) 817
XmaI CCCGGG 1 cut(s) 610
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.