MD10G1018000.v1.1

VQ motif

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0003162
Physical Location & Seq
Forward (+)
2306805 .. 2307482
678 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1018000.v1.1.491

Sequence Viewer

Length: 678 bp
ATGGATTATTCAACAGGAAAATCTCCAAGAAGAGAGCTTCAACTCCAAGGTCCACGTCCAACGCCTCTCAAGATACACAAAGACTCTCACAAGATCAAGAAACCTCCAGTTGTACCACAACCGTCACAACTACCAAACCAGCAGCAGCAGCCCCATCACTACCAGCAGCCACGTCAACCGGTCATAATCTACACGGTGTCCCCGAAGGTCATCCACACAAACCCTAGCGAGTTCATGGACCTTGTCCAACGCCTCACCGGCTTACCACCATCGAATTCTTCTACTACATCTTCTTCCCCGCCTAGAAATAACAATATTCATCCTCCGAATTACGTTAACTATAATAATCCTCCAGCTCATGATGATCAAGGGATGAAGAAAACTAGTACTGCTCAAGATGTTGTGGAAGAAATGGAAATTATGGATGGTGGTGGTGGTGGTGATGGTGGTGTGAGGCAAAAGGGTTTGTTTCCAGGGATCTTGTCGCCTGGGCCCGCTTCGCTTACTCCGATTCCTTCAAACTTCTTCTCTCCACCGGCGGATAGCTTCTTTCATGATTTGAGCCCCGTGCTCCATGGCAACAGGAACTTCATAGAAGGTAGTTTCATGCCTAGTCCGTCAAACTTCTTTTCCCCGTCTACATCCATAGACTTATTCAACAATTTTCCATATCAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04016 map04016
Pfam Domains
Protein Families
VQ

Protein Analysis

226

Amino Acids

24.83

Weight (kDa)

8.03

Isoelectric Point (pI)

75.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VQ PF05678 65 - 90 6.5e-13 VQ motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 638
AciI CCGC 3 cut(s) 299, 495, 539
AclWI GGATC 1 cut(s) 485
AcsI RAATTY 1 cut(s) 274
AfaI GTAC 2 cut(s) 114, 388
AgeI ACCGGT 1 cut(s) 178
AgsI TTSAA 4 cut(s) 12, 41, 519, 658
AhlI ACTAGT 1 cut(s) 383
AjiI CACGTC 2 cut(s) 56, 173
AjnI CCWGG 2 cut(s) 472, 487
AluBI AGCT 3 cut(s) 37, 356, 546
AluI AGCT 3 cut(s) 37, 356, 546
Alw21I GWGCWC 1 cut(s) 573
AlwI GGATC 1 cut(s) 485
AoxI GGCC 1 cut(s) 491
ApaI GGGCCC 1 cut(s) 495
ApeKI GCWGC 4 cut(s) 142, 145, 148, 166
ApoI RAATTY 1 cut(s) 274
AsiGI ACCGGT 1 cut(s) 178
AspS9I GGNCC 4 cut(s) 50, 238, 491, 492
AsuHPI GGTGA 2 cut(s) 247, 452
AvaII GGWCC 2 cut(s) 50, 238
BaeGI GKGCMC 1 cut(s) 495
BanII GRGCYC 2 cut(s) 495, 566
Bbv12I GWGCWC 1 cut(s) 573
BbvI GCAGC 4 cut(s) 154, 157, 160, 178
BccI CCATC 4 cut(s) 162, 277, 419, 437
BciT130I CCWGG 2 cut(s) 474, 489
BclI TGATCA 1 cut(s) 364
BcuI ACTAGT 1 cut(s) 383
BfaI CTAG 4 cut(s) 225, 303, 384, 612
BglI GCCNNNNNGGC 1 cut(s) 258
BisI GCNGC 4 cut(s) 143, 146, 149, 167
BlsI GCNGC 4 cut(s) 144, 147, 150, 168
BmcAI AGTACT 1 cut(s) 388
Bme1390I CCNGG 2 cut(s) 474, 489
Bme18I GGWCC 2 cut(s) 50, 238
BmgBI CACGTC 2 cut(s) 56, 173
BmgT120I GGNCC 4 cut(s) 50, 238, 491, 492
BmiI GGNNCC 1 cut(s) 493
BmrFI CCNGG 2 cut(s) 474, 489
BpmI CTGGAG 2 cut(s) 90, 336
BpuEI CTTGAG 2 cut(s) 53, 378
BsaJI CCNNGG 4 cut(s) 46, 473, 488, 574
BsaWI WCCGGW 1 cut(s) 178
Bse118I RCCGGY 3 cut(s) 178, 257, 535
Bse1I ACTGG 1 cut(s) 107
BseBI CCWGG 2 cut(s) 474, 489
BseDI CCNNGG 4 cut(s) 46, 473, 488, 574
BseGI GGATG 5 cut(s) 210, 319, 378, 430, 641
BseNI ACTGG 1 cut(s) 107
BseSI GKGCMC 1 cut(s) 495
BseXI GCAGC 4 cut(s) 154, 157, 160, 178
BshFI GGCC 1 cut(s) 493
BshTI ACCGGT 1 cut(s) 178
BsiHKAI GWGCWC 1 cut(s) 573
BsiSI CCGG 3 cut(s) 179, 258, 536
BslFI GGGAC 1 cut(s) 184
BsmFI GGGAC 1 cut(s) 184
BsnI GGCC 1 cut(s) 493
Bsp120I GGGCCC 1 cut(s) 491
Bsp1286I GDGCHC 3 cut(s) 495, 566, 573
Bsp143I GATC 3 cut(s) 93, 364, 477
Bsp19I CCATGG 1 cut(s) 574
BspACI CCGC 3 cut(s) 299, 495, 539
BspANI GGCC 1 cut(s) 493
BspHI TCATGA 2 cut(s) 358, 553
BspLI GGNNCC 1 cut(s) 493
BspPI GGATC 1 cut(s) 485
BsrFI RCCGGY 3 cut(s) 178, 257, 535
BsrI ACTGG 1 cut(s) 107
BssAI RCCGGY 3 cut(s) 178, 257, 535
BssECI CCNNGG 4 cut(s) 46, 473, 488, 574
BssMI GATC 3 cut(s) 93, 364, 477
BssT1I CCWWGG 2 cut(s) 46, 574
Bst2UI CCWGG 2 cut(s) 474, 489
Bst4CI ACNGT 2 cut(s) 123, 196
Bst6I CTCTTC 1 cut(s) 25
BstC8I GCNNGC 1 cut(s) 495
BstDSI CCRYGG 1 cut(s) 574
BstF5I GGATG 5 cut(s) 210, 319, 378, 430, 641
BstKTI GATC 3 cut(s) 96, 367, 480
BstMBI GATC 3 cut(s) 93, 364, 477
BstMWI GCNNNNNNNGC 3 cut(s) 148, 258, 499
BstNI CCWGG 2 cut(s) 474, 489
BstSCI CCNGG 2 cut(s) 472, 487
BstSLI GKGCMC 1 cut(s) 495
BstV1I GCAGC 4 cut(s) 154, 157, 160, 178
BstX2I RGATCY 1 cut(s) 477
BstYI RGATCY 1 cut(s) 477
BsuRI GGCC 1 cut(s) 493
BtgI CCRYGG 1 cut(s) 574
BtrI CACGTC 2 cut(s) 56, 173
BtsCI GGATG 5 cut(s) 210, 319, 378, 430, 641
Cac8I GCNNGC 1 cut(s) 495
CciI TCATGA 2 cut(s) 358, 553
Cfr10I RCCGGY 3 cut(s) 178, 257, 535
Cfr13I GGNCC 4 cut(s) 50, 238, 491, 492
Csp6I GTAC 2 cut(s) 113, 387
CspAI ACCGGT 1 cut(s) 178
CviAII CATG 5 cut(s) 235, 359, 554, 575, 607
CviJI RGCY 8 cut(s) 37, 151, 169, 261, 356, 493, 546, 564
CviKI_1 RGCY 8 cut(s) 37, 151, 169, 261, 356, 493, 546, 564
CviQI GTAC 2 cut(s) 113, 387
DpnI GATC 3 cut(s) 95, 366, 479
DpnII GATC 3 cut(s) 93, 364, 477
Eam1104I CTCTTC 1 cut(s) 25
EarI CTCTTC 1 cut(s) 25
EciI GGCGGA 1 cut(s) 554
Eco130I CCWWGG 2 cut(s) 46, 574
Eco24I GRGCYC 2 cut(s) 495, 566
Eco47I GGWCC 2 cut(s) 50, 238
EcoRI GAATTC 1 cut(s) 274
EcoRII CCWGG 2 cut(s) 472, 487
EcoT14I CCWWGG 2 cut(s) 46, 574
EcoT38I GRGCYC 2 cut(s) 495, 566
ErhI CCWWGG 2 cut(s) 46, 574
FaeI CATG 5 cut(s) 238, 362, 557, 578, 610
FaqI GGGAC 1 cut(s) 184
FatI CATG 5 cut(s) 234, 358, 553, 574, 606
FauI CCCGC 2 cut(s) 306, 502
FbaI TGATCA 1 cut(s) 364
FblI GTMKAC 1 cut(s) 638
Fnu4HI GCNGC 4 cut(s) 143, 146, 149, 167
FokI GGATG 5 cut(s) 197, 306, 385, 437, 628
FriOI GRGCYC 2 cut(s) 495, 566
Fsp4HI GCNGC 4 cut(s) 143, 146, 149, 167
FspBI CTAG 4 cut(s) 225, 303, 384, 612
GluI GCNGC 4 cut(s) 143, 146, 149, 167
GsuI CTGGAG 2 cut(s) 90, 336
HaeIII GGCC 1 cut(s) 493
HapII CCGG 3 cut(s) 179, 258, 536
Hin1II CATG 5 cut(s) 238, 362, 557, 578, 610
HincII GTYRAC 2 cut(s) 176, 337
HindII GTYRAC 2 cut(s) 176, 337
HinfI GANTC 2 cut(s) 83, 511
HpaI GTTAAC 1 cut(s) 337
HpaII CCGG 3 cut(s) 179, 258, 536
HphI GGTGA 2 cut(s) 247, 452
Hpy166II GTNNAC 4 cut(s) 53, 176, 337, 639
Hpy188I TCNGA 2 cut(s) 327, 510
Hpy188III TCNNGA 5 cut(s) 70, 97, 359, 395, 554
Hpy8I GTNNAC 4 cut(s) 53, 176, 337, 639
HpyAV CCTTC 3 cut(s) 199, 525, 590
HpyCH4III ACNGT 2 cut(s) 123, 196
HpyCH4IV ACGT 3 cut(s) 55, 172, 333
HpyF10VI GCNNNNNNNGC 3 cut(s) 148, 258, 499
HpySE526I ACGT 3 cut(s) 55, 172, 333
Hsp92II CATG 5 cut(s) 238, 362, 557, 578, 610
Ksp22I TGATCA 1 cut(s) 364
KspAI GTTAAC 1 cut(s) 337
Kzo9I GATC 3 cut(s) 93, 364, 477
LmnI GCTCC 1 cut(s) 576
Lsp1109I GCAGC 4 cut(s) 154, 157, 160, 178
MaeI CTAG 4 cut(s) 225, 303, 384, 612
MaeII ACGT 3 cut(s) 55, 172, 333
MaeIII GTNAC 1 cut(s) 123
MalI GATC 3 cut(s) 95, 366, 479
MboI GATC 3 cut(s) 93, 364, 477
MboII GAAGA 7 cut(s) 42, 270, 282, 285, 388, 419, 517
MflI RGATCY 1 cut(s) 477
MhlI GDGCHC 3 cut(s) 495, 566, 573
MluCI AATT 4 cut(s) 274, 328, 417, 661
MlyI GAGTC 1 cut(s) 77
MmeI TCCRAC 2 cut(s) 83, 271
MnlI CCTC 6 cut(s) 75, 114, 263, 333, 360, 447
MseI TTAA 1 cut(s) 336
MspI CCGG 3 cut(s) 179, 258, 536
MspR9I CCNGG 2 cut(s) 474, 489
MvaI CCWGG 2 cut(s) 474, 489
MwoI GCNNNNNNNGC 3 cut(s) 148, 258, 499
NcoI CCATGG 1 cut(s) 574
NdeII GATC 3 cut(s) 93, 364, 477
NlaIII CATG 5 cut(s) 238, 362, 557, 578, 610
NlaIV GGNNCC 1 cut(s) 493
NmuCI GTSAC 1 cut(s) 123
PagI TCATGA 2 cut(s) 358, 553
PcsI WCGNNNNNNNCGW 2 cut(s) 200, 506
PfeI GAWTC 1 cut(s) 511
PflFI GACNNNGTC 1 cut(s) 242
PinAI ACCGGT 1 cut(s) 178
PkrI GCNGC 4 cut(s) 144, 147, 150, 168
PleI GAGTC 1 cut(s) 77
PpsI GAGTC 1 cut(s) 77
Psp6I CCWGG 2 cut(s) 472, 487
PspGI CCWGG 2 cut(s) 472, 487
PspN4I GGNNCC 1 cut(s) 493
PspOMI GGGCCC 1 cut(s) 491
PspPI GGNCC 4 cut(s) 50, 238, 491, 492
PsuI RGATCY 1 cut(s) 477
PsyI GACNNNGTC 1 cut(s) 242
RsaI GTAC 2 cut(s) 114, 388
RsaNI GTAC 2 cut(s) 113, 387
SaqAI TTAA 1 cut(s) 336
SatI GCNGC 4 cut(s) 143, 146, 149, 167
Sau3AI GATC 3 cut(s) 93, 364, 477
Sau96I GGNCC 4 cut(s) 50, 238, 491, 492
ScaI AGTACT 1 cut(s) 388
SchI GAGTC 1 cut(s) 77
ScrFI CCNGG 2 cut(s) 474, 489
SduI GDGCHC 3 cut(s) 495, 566, 573
SgrAI CRCCGGYG 1 cut(s) 535
SinI GGWCC 2 cut(s) 50, 238
SmlI CTYRAG 2 cut(s) 68, 393
SmoI CTYRAG 2 cut(s) 68, 393
SpeI ACTAGT 1 cut(s) 383
Sse9I AATT 4 cut(s) 274, 328, 417, 661
SsiI CCGC 3 cut(s) 299, 495, 539
SspI AATATT 1 cut(s) 316
SspMI CTAG 4 cut(s) 225, 303, 384, 612
StyD4I CCNGG 2 cut(s) 472, 487
StyI CCWWGG 2 cut(s) 46, 574
TaaI ACNGT 2 cut(s) 123, 196
TaiI ACGT 3 cut(s) 58, 175, 336
TaqI TCGA 1 cut(s) 272
TasI AATT 4 cut(s) 274, 328, 417, 661
TatI WGTACW 1 cut(s) 386
TfiI GAWTC 1 cut(s) 511
Tru1I TTAA 1 cut(s) 336
Tru9I TTAA 1 cut(s) 336
TseFI GTSAC 1 cut(s) 123
TseI GCWGC 4 cut(s) 142, 145, 148, 166
Tsp45I GTSAC 1 cut(s) 123
TspDTI ATGAA 6 cut(s) 223, 308, 389, 542, 580, 595
TspGWI ACGGA 1 cut(s) 606
Tth111I GACNNNGTC 1 cut(s) 242
VpaK11BI GGWCC 2 cut(s) 50, 238
XapI RAATTY 1 cut(s) 274
XmiI GTMKAC 1 cut(s) 638
XspI CTAG 4 cut(s) 225, 303, 384, 612
ZrmI AGTACT 1 cut(s) 388
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.