pycom10g02690

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0003162
Physical Location & Seq
Reverse (-)
3030294 .. 3031494
1201 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g02690.1

Sequence Viewer

Length: 1101 bp
ATGAAGGTTAGTAGGGTCGATGAGGAACTGGTTGATTCACAAAACAAACTTCTTTCTGATATCTTGAGATCGGGAAACATGAAGGTTAGTAGGGTCGATGAAGGGACCAAGGAGATTAAAAGAAGACTTGGCAACAAAAGAGTACTTGTCATAGTAGATGATGTAGACAGCGATGCACAATTAGATGCATTAGCTATAAAGCATGACTCATTTGGTCCAGGAAGTAGAATTATTATAACAACAAGGGATCAACATTTGCTAAAGATACTTAATGTGGAGGCGACATGTACTGTGCAAGAAATGAATAAAGAAGAAGCTCTTGAGCTACTTAGTTGGCATGCCTTTGGAAAGAATTATCCTAATGAAGAGTATGTTGAGCTCTCAAGAAAAGTTGTTGGCTACTGTGGAGGTTTGCCACTAGCACTTGAAGTCTTAGGTTCTTGTCTGCATACAAAAAGCAGAAGTGAATGGAAAACTGAACTGGATAAATGGAAAAGATTACAGCCTTATTTCACCTTCAACGGCATGGATTATTCAATAGAAAAATATCCGAGAAGAAAGCTTCAACTCAAAGGTCCACGTCCAACGCCTCTCAACATAAACAAAGACTCAGTCAAGATCCAGAAACCTCCAGTTGTACGCTCAAGTCCCCGAAGGTCATACGCTCAAGATTACAGCCTTATTTCACCGTCACCGGTCGTAATCTACTCAGTGTCCCCGAAGGTCATACGCTCAAGTCCTAGCGGGCTCATGGACCTTGTCCAAAGCCTCACTGGCTTACCACCATCAAATTATTCCACTACATCTTCTTCCCTGCCCAGTAATATTAATCCTCCGAATTACGATAACTTTAATAAACCTCCGGCTCATGATCAAGGGATAAATAAAAGCAGTACTGCTCGAGATGTTGTGGAAGGAATGGAAATAATGGATGATGGTGGTGATGGTTGTGTGAGGCAAAAGGGTGTGTTTCGGGTGATCTTGTCGCCTGGGCCCGCTTCGATTACTCCGATTCCTTCAAACTTCTTCTCGCTGGGCCCGCTTCGATTACTCCGACTCCTTCAAACTTCTTCTCTCCGCCGGCGGGAGTCATACCTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

367

Amino Acids

40.97

Weight (kDa)

9.31

Isoelectric Point (pI)

68.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 25 - 117 5.5e-11 NB-ARC domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 236
AccI GTMKAC 1 cut(s) 165
AciI CCGC 5 cut(s) 744, 996, 1040, 1078, 1084
AclWI GGATC 2 cut(s) 255, 613
AfaI GTAC 4 cut(s) 144, 289, 639, 895
AfiI CCNNNNNNNGG 1 cut(s) 1084
AflIII ACRYGT 1 cut(s) 284
AgeI ACCGGT 1 cut(s) 694
AgsI TTSAA 6 cut(s) 428, 520, 537, 566, 1020, 1064
AjiI CACGTC 1 cut(s) 581
AjnI CCWGG 2 cut(s) 217, 988
AluBI AGCT 5 cut(s) 194, 317, 325, 379, 562
AluI AGCT 5 cut(s) 194, 317, 325, 379, 562
Alw21I GWGCWC 1 cut(s) 381
AlwI GGATC 2 cut(s) 255, 613
Ama87I CYCGRG 1 cut(s) 900
AoxI GGCC 2 cut(s) 992, 1036
ApaI GGGCCC 2 cut(s) 996, 1040
ArsI GACNNNNNNTTYG 2 cut(s) 597, 629
AseI ATTAAT 1 cut(s) 828
AsiGI ACCGGT 1 cut(s) 694
AspS9I GGNCC 8 cut(s) 105, 215, 575, 754, 992, 993, 1036, 1037
AsuHPI GGTGA 5 cut(s) 505, 678, 684, 953, 988
AvaI CYCGRG 1 cut(s) 900
AvaII GGWCC 4 cut(s) 105, 215, 575, 754
BaeGI GKGCMC 2 cut(s) 996, 1040
BanII GRGCYC 4 cut(s) 381, 750, 996, 1040
BbsI GAAGAC 1 cut(s) 130
Bbv12I GWGCWC 1 cut(s) 381
BccI CCATC 3 cut(s) 793, 929, 938
BceAI ACGGC 1 cut(s) 538
BciT130I CCWGG 2 cut(s) 219, 990
BclI TGATCA 1 cut(s) 871
BfaI CTAG 2 cut(s) 419, 741
BglI GCCNNNNNGGC 1 cut(s) 774
BmcAI AGTACT 2 cut(s) 144, 895
Bme1390I CCNGG 2 cut(s) 219, 990
Bme18I GGWCC 4 cut(s) 105, 215, 575, 754
BmeT110I CYCGRG 1 cut(s) 900
BmgBI CACGTC 1 cut(s) 581
BmgT120I GGNCC 8 cut(s) 105, 215, 575, 754, 992, 993, 1036, 1037
BmiI GGNNCC 3 cut(s) 106, 994, 1038
BmrFI CCNGG 2 cut(s) 219, 990
BmrI ACTGGG 1 cut(s) 813
BmsI GCATC 2 cut(s) 163, 175
BmuI ACTGGG 1 cut(s) 813
BpiI GAAGAC 1 cut(s) 130
BpmI CTGGAG 1 cut(s) 615
BpuEI CTTGAG 6 cut(s) 85, 341, 367, 628, 651, 718
BsaJI CCNNGG 2 cut(s) 108, 989
BsaWI WCCGGW 1 cut(s) 694
BsaXI ACNNNNNCTCC 2 cut(s) 1041, 1071
Bsc4I CCNNNNNNNGG 1 cut(s) 1084
Bse118I RCCGGY 2 cut(s) 694, 1080
Bse1I ACTGG 5 cut(s) 33, 486, 632, 778, 819
BseBI CCWGG 2 cut(s) 219, 990
BseDI CCNNGG 2 cut(s) 108, 989
BseGI GGATG 1 cut(s) 937
BseLI CCNNNNNNNGG 1 cut(s) 1084
BseMII CTCAG 2 cut(s) 624, 723
BseNI ACTGG 5 cut(s) 33, 486, 632, 778, 819
BseSI GKGCMC 2 cut(s) 996, 1040
BseYI CCCAGC 1 cut(s) 1033
Bsh1285I CGRYCG 1 cut(s) 699
BshFI GGCC 2 cut(s) 994, 1038
BshTI ACCGGT 1 cut(s) 694
BsiEI CGRYCG 1 cut(s) 699
BsiHKAI GWGCWC 1 cut(s) 381
BsiHKCI CYCGRG 1 cut(s) 900
BsiSI CCGG 3 cut(s) 695, 863, 1081
BslFI GGGAC 3 cut(s) 118, 633, 700
BslI CCNNNNNNNGG 1 cut(s) 1084
BsmFI GGGAC 3 cut(s) 118, 633, 700
BsnI GGCC 2 cut(s) 994, 1038
BsoBI CYCGRG 1 cut(s) 900
Bsp120I GGGCCC 2 cut(s) 992, 1036
Bsp1286I GDGCHC 4 cut(s) 381, 750, 996, 1040
Bsp143I GATC 5 cut(s) 68, 247, 618, 871, 978
BspACI CCGC 5 cut(s) 744, 996, 1040, 1078, 1084
BspANI GGCC 2 cut(s) 994, 1038
BspCNI CTCAG 2 cut(s) 623, 722
BspHI TCATGA 1 cut(s) 868
BspLI GGNNCC 3 cut(s) 106, 994, 1038
BspPI GGATC 2 cut(s) 255, 613
BsrFI RCCGGY 2 cut(s) 694, 1080
BsrI ACTGG 5 cut(s) 33, 486, 632, 778, 819
BssAI RCCGGY 2 cut(s) 694, 1080
BssECI CCNNGG 2 cut(s) 108, 989
BssMI GATC 5 cut(s) 68, 247, 618, 871, 978
BssT1I CCWWGG 1 cut(s) 108
Bst2UI CCWGG 2 cut(s) 219, 990
Bst4CI ACNGT 3 cut(s) 292, 404, 690
Bst6I CTCTTC 1 cut(s) 360
BstC8I GCNNGC 5 cut(s) 339, 746, 996, 1040, 1082
BstDEI CTNAG 4 cut(s) 329, 433, 610, 709
BstF5I GGATG 1 cut(s) 937
BstKTI GATC 5 cut(s) 71, 250, 621, 874, 981
BstMBI GATC 5 cut(s) 68, 247, 618, 871, 978
BstMCI CGRYCG 1 cut(s) 699
BstMWI GCNNNNNNNGC 2 cut(s) 774, 1039
BstNI CCWGG 2 cut(s) 219, 990
BstNSI RCATGY 2 cut(s) 288, 341
BstSCI CCNGG 2 cut(s) 217, 988
BstSLI GKGCMC 2 cut(s) 996, 1040
BstV2I GAAGAC 1 cut(s) 130
BstX2I RGATCY 1 cut(s) 618
BstYI RGATCY 1 cut(s) 618
BsuRI GGCC 2 cut(s) 994, 1038
BtgZI GCGATG 1 cut(s) 186
BtrI CACGTC 1 cut(s) 581
BtsCI GGATG 1 cut(s) 937
BtsIMutI CAGTG 2 cut(s) 717, 771
Cac8I GCNNGC 5 cut(s) 339, 746, 996, 1040, 1082
CciI TCATGA 1 cut(s) 868
Cfr10I RCCGGY 2 cut(s) 694, 1080
Cfr13I GGNCC 8 cut(s) 105, 215, 575, 754, 992, 993, 1036, 1037
Csp6I GTAC 4 cut(s) 143, 288, 638, 894
CspAI ACCGGT 1 cut(s) 694
CviAII CATG 7 cut(s) 79, 203, 285, 338, 526, 751, 869
CviQI GTAC 4 cut(s) 143, 288, 638, 894
DdeI CTNAG 4 cut(s) 329, 433, 610, 709
DpnI GATC 5 cut(s) 70, 249, 620, 873, 980
DpnII GATC 5 cut(s) 68, 247, 618, 871, 978
Eam1104I CTCTTC 1 cut(s) 360
EarI CTCTTC 1 cut(s) 360
EciI GGCGGA 1 cut(s) 1067
Ecl136II GAGCTC 1 cut(s) 379
Eco130I CCWWGG 1 cut(s) 108
Eco24I GRGCYC 4 cut(s) 381, 750, 996, 1040
Eco32I GATATC 1 cut(s) 61
Eco47I GGWCC 4 cut(s) 105, 215, 575, 754
Eco53kI GAGCTC 1 cut(s) 379
Eco88I CYCGRG 1 cut(s) 900
EcoICRI GAGCTC 1 cut(s) 379
EcoRII CCWGG 2 cut(s) 217, 988
EcoRV GATATC 1 cut(s) 61
EcoT14I CCWWGG 1 cut(s) 108
EcoT22I ATGCAT 1 cut(s) 190
EcoT38I GRGCYC 4 cut(s) 381, 750, 996, 1040
ErhI CCWWGG 1 cut(s) 108
FaeI CATG 7 cut(s) 82, 206, 288, 341, 529, 754, 872
FalI AAGNNNNNCTT 2 cut(s) 303, 335
FaqI GGGAC 3 cut(s) 118, 633, 700
FatI CATG 7 cut(s) 78, 202, 284, 337, 525, 750, 868
FauI CCCGC 4 cut(s) 737, 1003, 1047, 1077
FbaI TGATCA 1 cut(s) 871
FblI GTMKAC 1 cut(s) 165
FokI GGATG 1 cut(s) 944
FriOI GRGCYC 4 cut(s) 381, 750, 996, 1040
FspBI CTAG 2 cut(s) 419, 741
GsaI CCCAGC 1 cut(s) 1037
GsuI CTGGAG 1 cut(s) 615
HaeIII GGCC 2 cut(s) 994, 1038
HapII CCGG 3 cut(s) 695, 863, 1081
Hin1II CATG 7 cut(s) 82, 206, 288, 341, 529, 754, 872
HindIII AAGCTT 1 cut(s) 560
HinfI GANTC 6 cut(s) 35, 206, 608, 1012, 1056, 1088
HpaII CCGG 3 cut(s) 695, 863, 1081
HphI GGTGA 5 cut(s) 505, 678, 684, 953, 988
Hpy166II GTNNAC 2 cut(s) 166, 578
Hpy188I TCNGA 5 cut(s) 58, 552, 837, 1011, 1055
Hpy188III TCNNGA 9 cut(s) 64, 72, 320, 384, 616, 622, 668, 869, 902
Hpy8I GTNNAC 2 cut(s) 166, 578
HpyAV CCTTC 8 cut(s) 76, 95, 526, 648, 715, 908, 1026, 1070
HpyCH4III ACNGT 3 cut(s) 292, 404, 690
HpyCH4IV ACGT 1 cut(s) 580
HpyCH4V TGCA 4 cut(s) 176, 188, 295, 448
HpyF10VI GCNNNNNNNGC 2 cut(s) 774, 1039
HpyF3I CTNAG 4 cut(s) 329, 433, 610, 709
HpySE526I ACGT 1 cut(s) 580
Hsp92II CATG 7 cut(s) 82, 206, 288, 341, 529, 754, 872
KroI GCCGGC 1 cut(s) 1080
KroNI GCCGGC 1 cut(s) 1082
Ksp22I TGATCA 1 cut(s) 871
Kzo9I GATC 5 cut(s) 68, 247, 618, 871, 978
LweI GCATC 2 cut(s) 163, 175
MaeI CTAG 2 cut(s) 419, 741
MaeII ACGT 1 cut(s) 580
MaeIII GTNAC 1 cut(s) 690
MalI GATC 5 cut(s) 70, 249, 620, 873, 980
MboI GATC 5 cut(s) 68, 247, 618, 871, 978
MboII GAAGA 8 cut(s) 135, 323, 377, 567, 798, 801, 1018, 1062
MflI RGATCY 1 cut(s) 618
MhlI GDGCHC 4 cut(s) 381, 750, 996, 1040
MluCI AATT 5 cut(s) 179, 228, 352, 790, 838
MlyI GAGTC 4 cut(s) 200, 602, 1050, 1097
MmeI TCCRAC 2 cut(s) 608, 1078
MnlI CCTC 9 cut(s) 16, 271, 401, 600, 639, 779, 843, 870, 948
Mph1103I ATGCAT 1 cut(s) 190
MreI CGCCGGCG 1 cut(s) 1080
MroNI GCCGGC 1 cut(s) 1080
MseI TTAA 4 cut(s) 117, 270, 828, 852
MspI CCGG 3 cut(s) 695, 863, 1081
MspR9I CCNGG 2 cut(s) 219, 990
MvaI CCWGG 2 cut(s) 219, 990
MwoI GCNNNNNNNGC 2 cut(s) 774, 1039
NaeI GCCGGC 1 cut(s) 1082
NdeII GATC 5 cut(s) 68, 247, 618, 871, 978
NgoMIV GCCGGC 1 cut(s) 1080
NlaIII CATG 7 cut(s) 82, 206, 288, 341, 529, 754, 872
NlaIV GGNNCC 3 cut(s) 106, 994, 1038
NmuCI GTSAC 1 cut(s) 690
NsiI ATGCAT 1 cut(s) 190
NspI RCATGY 2 cut(s) 288, 341
PaeI GCATGC 1 cut(s) 341
PaeR7I CTCGAG 1 cut(s) 900
PagI TCATGA 1 cut(s) 868
PciI ACATGT 1 cut(s) 284
PcsI WCGNNNNNNNCGW 2 cut(s) 1007, 1051
PdiI GCCGGC 1 cut(s) 1082
PfeI GAWTC 2 cut(s) 35, 1012
PflFI GACNNNGTC 2 cut(s) 611, 758
PfoI TCCNGGA 1 cut(s) 217
PinAI ACCGGT 1 cut(s) 694
PleI GAGTC 4 cut(s) 200, 602, 1050, 1096
PpsI GAGTC 4 cut(s) 200, 602, 1050, 1096
PscI ACATGT 1 cut(s) 284
PshBI ATTAAT 1 cut(s) 828
PsiI TTATAA 1 cut(s) 236
Psp124BI GAGCTC 1 cut(s) 381
Psp6I CCWGG 2 cut(s) 217, 988
PspFI CCCAGC 1 cut(s) 1033
PspGI CCWGG 2 cut(s) 217, 988
PspN4I GGNNCC 3 cut(s) 106, 994, 1038
PspOMI GGGCCC 2 cut(s) 992, 1036
PspPI GGNCC 8 cut(s) 105, 215, 575, 754, 992, 993, 1036, 1037
PsuI RGATCY 1 cut(s) 618
PsyI GACNNNGTC 2 cut(s) 611, 758
RsaI GTAC 4 cut(s) 144, 289, 639, 895
RsaNI GTAC 4 cut(s) 143, 288, 638, 894
SacI GAGCTC 1 cut(s) 381
SaqAI TTAA 4 cut(s) 117, 270, 828, 852
Sau3AI GATC 5 cut(s) 68, 247, 618, 871, 978
Sau96I GGNCC 8 cut(s) 105, 215, 575, 754, 992, 993, 1036, 1037
ScaI AGTACT 2 cut(s) 144, 895
SchI GAGTC 4 cut(s) 200, 602, 1050, 1097
ScrFI CCNGG 2 cut(s) 219, 990
SduI GDGCHC 4 cut(s) 381, 750, 996, 1040
SfaNI GCATC 2 cut(s) 163, 175
Sfr274I CTCGAG 1 cut(s) 900
SgrAI CRCCGGYG 1 cut(s) 1080
SinI GGWCC 4 cut(s) 105, 215, 575, 754
SlaI CTCGAG 1 cut(s) 900
SmlI CTYRAG 7 cut(s) 64, 320, 382, 643, 666, 733, 900
SmoI CTYRAG 7 cut(s) 64, 320, 382, 643, 666, 733, 900
SphI GCATGC 1 cut(s) 341
Sse9I AATT 5 cut(s) 179, 228, 352, 790, 838
SsiI CCGC 5 cut(s) 744, 996, 1040, 1078, 1084
SspI AATATT 1 cut(s) 826
SspMI CTAG 2 cut(s) 419, 741
SstI GAGCTC 1 cut(s) 381
StyD4I CCNGG 2 cut(s) 217, 988
StyI CCWWGG 1 cut(s) 108
TaaI ACNGT 3 cut(s) 292, 404, 690
TaiI ACGT 1 cut(s) 583
TaqI TCGA 5 cut(s) 18, 96, 901, 1001, 1045
TasI AATT 5 cut(s) 179, 228, 352, 790, 838
TatI WGTACW 3 cut(s) 142, 287, 893
TfiI GAWTC 2 cut(s) 35, 1012
Tru1I TTAA 4 cut(s) 117, 270, 828, 852
Tru9I TTAA 4 cut(s) 117, 270, 828, 852
TscAI CASTG 2 cut(s) 717, 778
TseFI GTSAC 1 cut(s) 690
Tsp45I GTSAC 1 cut(s) 690
TspDTI ATGAA 5 cut(s) 17, 95, 114, 317, 378
TspRI CASTG 2 cut(s) 717, 778
Tth111I GACNNNGTC 2 cut(s) 611, 758
VpaK11BI GGWCC 4 cut(s) 105, 215, 575, 754
VspI ATTAAT 1 cut(s) 828
XceI RCATGY 2 cut(s) 288, 341
XcmI CCANNNNNNNNNTGG 1 cut(s) 770
XhoI CTCGAG 1 cut(s) 900
XmiI GTMKAC 1 cut(s) 165
XspI CTAG 2 cut(s) 419, 741
ZrmI AGTACT 2 cut(s) 144, 895
Zsp2I ATGCAT 1 cut(s) 190
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.