RchiOBHm_Chr2g0120181

VQ motif

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0003162
Physical Location & Seq
Reverse (-)
32889032 .. 32890298
1267 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ49283

Sequence Viewer

Length: 675 bp
ATGAACCCGCCACCGCAGCGGCCCACCACCCCAAAAAAGGAGCTCCAACTCCAAGGCCCCCGCCCAGCCCCTCTCAAAGTCAGCAAAGACTCCGTCAAAATCAAGAAGCCGCCTCCGCACCCCCACCTCCGCCAAAACGCACCTCAACAACAGTTCCAGCCGCCGCAGCAGCACCAGGAGCCGGTGGTCATCTACACCGTCTCGCCCAAAGTCATTCACACCTCCGTCAACGAGTTCATGTCCCTCGTCCAACGCCTCACCGGCCCTTCCTCCTCTCCCCCCGGCGACCTCTCCCCCGCCGCCCGCCTCGCCTCCATTGAGAAAACCAGCCCCTCTTCCGATCAGAAGCACTTCCTGCCCGTAAATACCACCACAGCCACCGCCGCCATTGACGACGACTTTATGGATAGTATTATGGAGGGTATTGACTTGATGGGTCAGTTCCCGGGGATTCTGTCGCCGGCGCCGGCCACCATGCCGCCGATCCCAGCTGGGTTCTTCTCTCCGGCCGCCGAAGACCCGCAAAACTTCAACTGGCAAGGCGCCAACAACATCAACAGCGGCTTCAATAGTTTCATGCCGAGCCCTTCCTCGAATTCGGCTCTGTTTTCGGCTCCTCTGGTTTCTCCGAGCCCTTCTTCGTGGGACCTCTTCAACAATTTCTTGGACTTCTAG

Protein Analysis

224

Amino Acids

24.29

Weight (kDa)

6.18

Isoelectric Point (pI)

74.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VQ PF05678 66 - 93 1.4e-12 VQ motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 463, 542
AclWI GGATC 1 cut(s) 478
AcoI YGGCCR 2 cut(s) 468, 507
AcsI RAATTY 1 cut(s) 595
AcyI GRCGYC 2 cut(s) 464, 543
AfiI CCNNNNNNNGG 2 cut(s) 37, 181
AgsI TTSAA 3 cut(s) 532, 568, 655
AjnI CCWGG 1 cut(s) 174
AluBI AGCT 2 cut(s) 43, 491
AluI AGCT 2 cut(s) 43, 491
Alw21I GWGCWC 1 cut(s) 45
Alw26I GTCTC 1 cut(s) 205
AlwI GGATC 1 cut(s) 478
Ama87I CYCGRG 1 cut(s) 445
AoxI GGCC 5 cut(s) 20, 55, 262, 468, 507
ApeKI GCWGC 3 cut(s) 16, 166, 169
ApoI RAATTY 1 cut(s) 595
ArsI GACNNNNNNTTYG 2 cut(s) 78, 110
Asp700I GAANNNNTTC 1 cut(s) 350
AspLEI GCGC 2 cut(s) 466, 545
AspS9I GGNCC 4 cut(s) 21, 56, 263, 646
AsuC2I CCSGG 3 cut(s) 282, 446, 447
AsuHPI GGTGA 1 cut(s) 250
AvaI CYCGRG 1 cut(s) 445
AvaII GGWCC 1 cut(s) 646
BanI GGYRCC 2 cut(s) 463, 542
BanII GRGCYC 3 cut(s) 45, 587, 635
BbsI GAAGAC 1 cut(s) 522
Bbv12I GWGCWC 1 cut(s) 45
BbvI GCAGC 3 cut(s) 28, 178, 181
BccI CCATC 1 cut(s) 427
BciT130I CCWGG 1 cut(s) 176
BcnI CCSGG 3 cut(s) 282, 446, 447
BcoDI GTCTC 1 cut(s) 205
BfaI CTAG 1 cut(s) 673
BfoI RGCGCY 2 cut(s) 467, 546
BglI GCCNNNNNGGC 1 cut(s) 261
Bme1390I CCNGG 4 cut(s) 176, 282, 446, 447
Bme18I GGWCC 1 cut(s) 646
BmeT110I CYCGRG 1 cut(s) 445
BmgT120I GGNCC 4 cut(s) 21, 56, 263, 646
BmiI GGNNCC 6 cut(s) 58, 180, 465, 544, 615, 647
BmrFI CCNGG 4 cut(s) 176, 282, 446, 447
BpiI GAAGAC 1 cut(s) 522
BpuMI CCSGG 3 cut(s) 282, 446, 447
BsaHI GRCGYC 2 cut(s) 464, 543
BsaJI CCNNGG 4 cut(s) 52, 280, 445, 446
Bsc4I CCNNNNNNNGG 2 cut(s) 37, 181
Bse118I RCCGGY 4 cut(s) 181, 260, 460, 466
Bse1I ACTGG 1 cut(s) 539
BseBI CCWGG 1 cut(s) 176
BseDI CCNNGG 4 cut(s) 52, 280, 445, 446
BseLI CCNNNNNNNGG 2 cut(s) 37, 181
BseNI ACTGG 1 cut(s) 539
BseRI GAGGAG 2 cut(s) 262, 606
BseX3I CGGCCG 1 cut(s) 507
BseXI GCAGC 3 cut(s) 28, 178, 181
BseYI CCCAGC 3 cut(s) 64, 487, 491
Bsh1285I CGRYCG 1 cut(s) 510
BshFI GGCC 5 cut(s) 22, 57, 264, 470, 509
BshNI GGYRCC 2 cut(s) 463, 542
BsiEI CGRYCG 1 cut(s) 510
BsiHKAI GWGCWC 1 cut(s) 45
BsiHKCI CYCGRG 1 cut(s) 445
BsiSI CCGG 7 cut(s) 182, 261, 282, 446, 461, 467, 506
BslFI GGGAC 2 cut(s) 226, 659
BslI CCNNNNNNNGG 2 cut(s) 37, 181
BsmAI GTCTC 1 cut(s) 205
BsmBI CGTCTC 1 cut(s) 205
BsmFI GGGAC 2 cut(s) 226, 659
BsnI GGCC 5 cut(s) 22, 57, 264, 470, 509
BsoBI CYCGRG 1 cut(s) 445
Bsp1286I GDGCHC 3 cut(s) 45, 587, 635
Bsp143I GATC 2 cut(s) 340, 483
BspANI GGCC 5 cut(s) 22, 57, 264, 470, 509
BspLI GGNNCC 6 cut(s) 58, 180, 465, 544, 615, 647
BspPI GGATC 1 cut(s) 478
BspT107I GGYRCC 2 cut(s) 463, 542
BsrFI RCCGGY 4 cut(s) 181, 260, 460, 466
BsrI ACTGG 1 cut(s) 539
BssAI RCCGGY 4 cut(s) 181, 260, 460, 466
BssECI CCNNGG 4 cut(s) 52, 280, 445, 446
BssMI GATC 2 cut(s) 340, 483
BssNI GRCGYC 2 cut(s) 464, 543
BssT1I CCWWGG 1 cut(s) 52
Bst2UI CCWGG 1 cut(s) 176
Bst4CI ACNGT 2 cut(s) 153, 199
Bst6I CTCTTC 2 cut(s) 340, 656
BstACI GRCGYC 2 cut(s) 464, 543
BstAPI GCANNNNNTGC 1 cut(s) 355
BstC8I GCNNGC 3 cut(s) 304, 462, 468
BstH2I RGCGCY 2 cut(s) 467, 546
BstHHI GCGC 2 cut(s) 466, 545
BstKTI GATC 2 cut(s) 343, 486
BstMAI GTCTC 1 cut(s) 205
BstMBI GATC 2 cut(s) 340, 483
BstMCI CGRYCG 1 cut(s) 510
BstMWI GCNNNNNNNGC 9 cut(s) 16, 115, 166, 169, 178, 261, 308, 355, 383
BstNI CCWGG 1 cut(s) 176
BstSCI CCNGG 4 cut(s) 174, 280, 444, 445
BstV1I GCAGC 3 cut(s) 28, 178, 181
BstV2I GAAGAC 1 cut(s) 522
BstZI CGGCCG 1 cut(s) 507
BsuRI GGCC 5 cut(s) 22, 57, 264, 470, 509
Cac8I GCNNGC 3 cut(s) 304, 462, 468
CfoI GCGC 2 cut(s) 466, 545
Cfr10I RCCGGY 4 cut(s) 181, 260, 460, 466
Cfr13I GGNCC 4 cut(s) 21, 56, 263, 646
Cfr9I CCCGGG 1 cut(s) 445
CviAII CATG 3 cut(s) 238, 475, 577
DinI GGCGCC 2 cut(s) 465, 544
DpnI GATC 2 cut(s) 342, 485
DpnII GATC 2 cut(s) 340, 483
EaeI YGGCCR 2 cut(s) 468, 507
EagI CGGCCG 1 cut(s) 507
Eam1104I CTCTTC 2 cut(s) 340, 656
EarI CTCTTC 2 cut(s) 340, 656
EciI GGCGGA 1 cut(s) 119
Ecl136II GAGCTC 1 cut(s) 43
EclXI CGGCCG 1 cut(s) 507
Eco130I CCWWGG 1 cut(s) 52
Eco24I GRGCYC 3 cut(s) 45, 587, 635
Eco47I GGWCC 1 cut(s) 646
Eco52I CGGCCG 1 cut(s) 507
Eco53kI GAGCTC 1 cut(s) 43
Eco88I CYCGRG 1 cut(s) 445
EcoICRI GAGCTC 1 cut(s) 43
EcoO109I RGGNCCY 2 cut(s) 56, 646
EcoRI GAATTC 1 cut(s) 595
EcoRII CCWGG 1 cut(s) 174
EcoT14I CCWWGG 1 cut(s) 52
EcoT38I GRGCYC 3 cut(s) 45, 587, 635
EgeI GGCGCC 2 cut(s) 465, 544
EheI GGCGCC 2 cut(s) 465, 544
ErhI CCWWGG 1 cut(s) 52
Esp3I CGTCTC 1 cut(s) 205
FaeI CATG 3 cut(s) 241, 478, 580
FaiI YATR 5 cut(s) 239, 404, 416, 476, 578
FaqI GGGAC 2 cut(s) 226, 659
FatI CATG 3 cut(s) 237, 474, 576
FauI CCCGC 5 cut(s) 15, 68, 304, 311, 528
FriOI GRGCYC 3 cut(s) 45, 587, 635
FspBI CTAG 1 cut(s) 673
GlaI GCGC 2 cut(s) 465, 544
GsaI CCCAGC 3 cut(s) 68, 491, 495
HaeII RGCGCY 2 cut(s) 467, 546
HaeIII GGCC 5 cut(s) 22, 57, 264, 470, 509
HapII CCGG 7 cut(s) 182, 261, 282, 446, 461, 467, 506
HhaI GCGC 2 cut(s) 466, 545
Hin1I GRCGYC 2 cut(s) 464, 543
Hin1II CATG 3 cut(s) 241, 478, 580
Hin6I GCGC 2 cut(s) 464, 543
HinP1I GCGC 2 cut(s) 464, 543
HincII GTYRAC 1 cut(s) 229
HindII GTYRAC 1 cut(s) 229
HinfI GANTC 2 cut(s) 89, 451
HpaII CCGG 7 cut(s) 182, 261, 282, 446, 461, 467, 506
HphI GGTGA 1 cut(s) 250
Hpy166II GTNNAC 1 cut(s) 229
Hpy188I TCNGA 3 cut(s) 340, 345, 630
Hpy188III TCNNGA 1 cut(s) 103
Hpy8I GTNNAC 1 cut(s) 229
Hpy99I CGWCG 1 cut(s) 398
HpyAV CCTTC 3 cut(s) 276, 597, 645
HpyCH4III ACNGT 2 cut(s) 153, 199
HpyF10VI GCNNNNNNNGC 9 cut(s) 16, 115, 166, 169, 178, 261, 308, 355, 383
Hsp92I GRCGYC 2 cut(s) 464, 543
Hsp92II CATG 3 cut(s) 241, 478, 580
HspAI GCGC 2 cut(s) 464, 543
KasI GGCGCC 2 cut(s) 463, 542
KroI GCCGGC 2 cut(s) 460, 466
KroNI GCCGGC 2 cut(s) 462, 468
Kzo9I GATC 2 cut(s) 340, 483
LmnI GCTCC 4 cut(s) 40, 48, 178, 619
Lsp1109I GCAGC 3 cut(s) 28, 178, 181
MaeI CTAG 1 cut(s) 673
MalI GATC 2 cut(s) 342, 485
MboI GATC 2 cut(s) 340, 483
MboII GAAGA 5 cut(s) 327, 490, 527, 630, 643
MhlI GDGCHC 3 cut(s) 45, 587, 635
MluCI AATT 2 cut(s) 595, 658
Mly113I GGCGCC 2 cut(s) 464, 543
MlyI GAGTC 1 cut(s) 83
MmeI TCCRAC 2 cut(s) 70, 274
MreI CGCCGGCG 1 cut(s) 460
MroNI GCCGGC 2 cut(s) 460, 466
MroXI GAANNNNTTC 1 cut(s) 350
MspA1I CMGCKG 3 cut(s) 19, 491, 561
MspI CCGG 7 cut(s) 182, 261, 282, 446, 461, 467, 506
MspR9I CCNGG 4 cut(s) 176, 282, 446, 447
MvaI CCWGG 1 cut(s) 176
MwoI GCNNNNNNNGC 9 cut(s) 16, 115, 166, 169, 178, 261, 308, 355, 383
NaeI GCCGGC 2 cut(s) 462, 468
NarI GGCGCC 2 cut(s) 464, 543
NciI CCSGG 3 cut(s) 282, 446, 447
NdeII GATC 2 cut(s) 340, 483
NgoMIV GCCGGC 2 cut(s) 460, 466
NlaIII CATG 3 cut(s) 241, 478, 580
NlaIV GGNNCC 6 cut(s) 58, 180, 465, 544, 615, 647
NmeAIII GCCGAG 1 cut(s) 606
PdiI GCCGGC 2 cut(s) 462, 468
PdmI GAANNNNTTC 1 cut(s) 350
PfeI GAWTC 1 cut(s) 451
PflFI GACNNNGTC 1 cut(s) 92
PleI GAGTC 1 cut(s) 83
PluTI GGCGCC 2 cut(s) 467, 546
PpsI GAGTC 1 cut(s) 83
PpuMI RGGWCCY 1 cut(s) 646
Psp124BI GAGCTC 1 cut(s) 45
Psp5II RGGWCCY 1 cut(s) 646
Psp6I CCWGG 1 cut(s) 174
PspFI CCCAGC 3 cut(s) 64, 487, 491
PspGI CCWGG 1 cut(s) 174
PspN4I GGNNCC 6 cut(s) 58, 180, 465, 544, 615, 647
PspPI GGNCC 4 cut(s) 21, 56, 263, 646
PspPPI RGGWCCY 1 cut(s) 646
PsyI GACNNNGTC 1 cut(s) 92
PvuII CAGCTG 1 cut(s) 491
SacI GAGCTC 1 cut(s) 45
Sau3AI GATC 2 cut(s) 340, 483
Sau96I GGNCC 4 cut(s) 21, 56, 263, 646
SchI GAGTC 1 cut(s) 83
ScrFI CCNGG 4 cut(s) 176, 282, 446, 447
SduI GDGCHC 3 cut(s) 45, 587, 635
SetI ASST 7 cut(s) 45, 129, 145, 224, 291, 493, 651
SfoI GGCGCC 2 cut(s) 465, 544
SgrAI CRCCGGYG 1 cut(s) 460
SinI GGWCC 1 cut(s) 646
SmaI CCCGGG 1 cut(s) 447
Sse9I AATT 2 cut(s) 595, 658
SspDI GGCGCC 2 cut(s) 463, 542
SspMI CTAG 1 cut(s) 673
SstI GAGCTC 1 cut(s) 45
StyD4I CCNGG 4 cut(s) 174, 280, 444, 445
StyI CCWWGG 1 cut(s) 52
TaaI ACNGT 2 cut(s) 153, 199
TaqI TCGA 1 cut(s) 593
TasI AATT 2 cut(s) 595, 658
TauI GCSGC 9 cut(s) 22, 112, 163, 166, 302, 386, 481, 512, 564
TfiI GAWTC 1 cut(s) 451
TseI GCWGC 3 cut(s) 16, 166, 169
TspDTI ATGAA 3 cut(s) 17, 226, 565
TspGWI ACGGA 2 cut(s) 82, 214
TspMI CCCGGG 1 cut(s) 445
Tth111I GACNNNGTC 1 cut(s) 92
VpaK11BI GGWCC 1 cut(s) 646
XapI RAATTY 1 cut(s) 595
XmaI CCCGGG 1 cut(s) 445
XmnI GAANNNNTTC 1 cut(s) 350
XspI CTAG 1 cut(s) 673
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.