MD15G1338100.v1.1

VQ motif

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0003162
Physical Location & Seq
Forward (+)
38134687 .. 38135364
678 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1338100.v1.1.491

Sequence Viewer

Length: 678 bp
ATGAACCCAGCAAGGCCTCCTCCAGCACCCAAAAAGGAGATACAACTCCAAGGTCCCCGCCCTTCCCCTCTCAAAGTCAACAAAGACTCCGTCAAGATCAAGAAGCCACCGCCGCACCCACCCCCGCATCCAGCATCCACCCAGCATCCACCTCCACCTCCACCTCCACATCCACAGCCGGTGGTGATCTACTCAGTGTCTCCCAAAGTCATCCACACCACTGAAAACGAGTTCATGTCCTTAGTCCAACGCCTAACCGGCCCTTCCACCCCTTCGCCCTCCTCCTCCTCCCCTGACGGCCTCTCGCCGGCTGCAAGACTCGCCTCCGTACACAAAACAAGCCCTAATTCAACCTCGGGCCACAACAAGCCCGTGACCACTGATGACGACTTCATGACAAGTATTTTCAATAATCCTGAAGATCAAGGAGGCGGCGGCGGAGGGTCAGTGCAACTTGATGAAATGGGTCAGTTTCCGGGAATATTGTCTCCGCCGCCAGCGTCTCTGCCGGCGGTAACAAGTGGGTTCTTCTCACAGCATCATCAGCATGACGTCATAAGCAGCCCAATGTCGAATAATTGGATGGGGATGGCTTTTAGCCCTGGTCCGTCTTCAGCTTTGATATCTCCCAGTCCTTGCTCTTGGGACCTCTTTAACAATTTCTTGCACTACTTTTAA

Protein Analysis

226

Amino Acids

23.94

Weight (kDa)

7.2

Isoelectric Point (pI)

79.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VQ PF05678 66 - 91 8.8e-11 VQ motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 555
AcuI CTGAAG 2 cut(s) 438, 597
AcyI GRCGYC 1 cut(s) 552
AfaI GTAC 1 cut(s) 330
AfiI CCNNNNNNNGG 1 cut(s) 307
AgsI TTSAA 2 cut(s) 351, 409
AjnI CCWGG 1 cut(s) 601
AluBI AGCT 1 cut(s) 617
AluI AGCT 1 cut(s) 617
Alw26I GTCTC 3 cut(s) 204, 492, 507
Ama87I CYCGRG 1 cut(s) 355
AoxI GGCC 4 cut(s) 14, 259, 298, 358
ApeKI GCWGC 2 cut(s) 311, 561
ArsI GACNNNNNNTTYG 2 cut(s) 75, 107
AspS9I GGNCC 5 cut(s) 53, 260, 358, 605, 646
AsuC2I CCSGG 1 cut(s) 477
AsuHPI GGTGA 1 cut(s) 196
AvaI CYCGRG 1 cut(s) 355
AvaII GGWCC 3 cut(s) 53, 605, 646
BbsI GAAGAC 1 cut(s) 603
BbvI GCAGC 2 cut(s) 298, 573
BccI CCATC 2 cut(s) 577, 583
BceAI ACGGC 1 cut(s) 313
BciT130I CCWGG 1 cut(s) 603
BcnI CCSGG 1 cut(s) 477
BcoDI GTCTC 3 cut(s) 204, 492, 507
BglI GCCNNNNNGGC 1 cut(s) 258
BisI GCNGC 6 cut(s) 113, 312, 433, 436, 494, 562
BlsI GCNGC 6 cut(s) 114, 313, 434, 437, 495, 563
Bme1390I CCNGG 2 cut(s) 477, 603
Bme18I GGWCC 3 cut(s) 53, 605, 646
BmeT110I CYCGRG 1 cut(s) 355
BmgT120I GGNCC 5 cut(s) 53, 260, 358, 605, 646
BmiI GGNNCC 2 cut(s) 55, 647
BmrFI CCNGG 2 cut(s) 477, 603
BmrI ACTGGG 1 cut(s) 624
BmsI GCATC 4 cut(s) 136, 143, 154, 547
BmuI ACTGGG 1 cut(s) 624
BpiI GAAGAC 1 cut(s) 603
BpmI CTGGAG 1 cut(s) 6
BpuMI CCSGG 1 cut(s) 477
BsaHI GRCGYC 1 cut(s) 552
BsaJI CCNNGG 3 cut(s) 49, 354, 601
BsaXI ACNNNNNCTCC 4 cut(s) 71, 101, 432, 462
Bsc4I CCNNNNNNNGG 1 cut(s) 307
Bse118I RCCGGY 4 cut(s) 178, 257, 307, 508
Bse1I ACTGG 1 cut(s) 630
BseBI CCWGG 1 cut(s) 603
BseDI CCNNGG 3 cut(s) 49, 354, 601
BseGI GGATG 7 cut(s) 127, 134, 145, 169, 210, 588, 594
BseLI CCNNNNNNNGG 1 cut(s) 307
BseMII CTCAG 1 cut(s) 207
BseNI ACTGG 1 cut(s) 630
BseRI GAGGAG 4 cut(s) 9, 271, 274, 277
BseXI GCAGC 2 cut(s) 298, 573
BseYI CCCAGC 2 cut(s) 7, 141
BshFI GGCC 4 cut(s) 16, 261, 300, 360
BsiHKCI CYCGRG 1 cut(s) 355
BsiSI CCGG 5 cut(s) 179, 258, 308, 476, 509
BslFI GGGAC 2 cut(s) 39, 659
BslI CCNNNNNNNGG 1 cut(s) 307
BsmAI GTCTC 3 cut(s) 204, 492, 507
BsmBI CGTCTC 1 cut(s) 507
BsmFI GGGAC 2 cut(s) 39, 659
BsnI GGCC 4 cut(s) 16, 261, 300, 360
BsoBI CYCGRG 1 cut(s) 355
Bsp143I GATC 3 cut(s) 96, 186, 421
BspANI GGCC 4 cut(s) 16, 261, 300, 360
BspCNI CTCAG 1 cut(s) 206
BspHI TCATGA 1 cut(s) 393
BspLI GGNNCC 2 cut(s) 55, 647
BsrFI RCCGGY 4 cut(s) 178, 257, 307, 508
BsrI ACTGG 1 cut(s) 630
BssAI RCCGGY 4 cut(s) 178, 257, 307, 508
BssECI CCNNGG 3 cut(s) 49, 354, 601
BssMI GATC 3 cut(s) 96, 186, 421
BssNI GRCGYC 1 cut(s) 552
BssT1I CCWWGG 1 cut(s) 49
Bst2UI CCWGG 1 cut(s) 603
BstACI GRCGYC 1 cut(s) 552
BstC8I GCNNGC 3 cut(s) 309, 498, 510
BstDEI CTNAG 2 cut(s) 193, 241
BstF5I GGATG 7 cut(s) 127, 134, 145, 169, 210, 588, 594
BstKTI GATC 3 cut(s) 99, 189, 424
BstMAI GTCTC 3 cut(s) 204, 492, 507
BstMBI GATC 3 cut(s) 96, 186, 421
BstMWI GCNNNNNNNGC 4 cut(s) 112, 258, 320, 544
BstNI CCWGG 1 cut(s) 603
BstSCI CCNGG 2 cut(s) 475, 601
BstV1I GCAGC 2 cut(s) 298, 573
BstV2I GAAGAC 1 cut(s) 603
BsuRI GGCC 4 cut(s) 16, 261, 300, 360
BtsCI GGATG 7 cut(s) 127, 134, 145, 169, 210, 588, 594
BtsIMutI CAGTG 4 cut(s) 201, 219, 378, 453
Cac8I GCNNGC 3 cut(s) 309, 498, 510
CciI TCATGA 1 cut(s) 393
Cfr10I RCCGGY 4 cut(s) 178, 257, 307, 508
Cfr13I GGNCC 5 cut(s) 53, 260, 358, 605, 646
CseI GACGC 1 cut(s) 489
Csp6I GTAC 1 cut(s) 329
CviAII CATG 3 cut(s) 235, 394, 548
CviQI GTAC 1 cut(s) 329
DdeI CTNAG 2 cut(s) 193, 241
DpnI GATC 3 cut(s) 98, 188, 423
DpnII GATC 3 cut(s) 96, 186, 421
EciI GGCGGA 2 cut(s) 453, 480
Eco130I CCWWGG 1 cut(s) 49
Eco147I AGGCCT 1 cut(s) 16
Eco32I GATATC 1 cut(s) 624
Eco47I GGWCC 3 cut(s) 53, 605, 646
Eco57I CTGAAG 2 cut(s) 438, 597
Eco88I CYCGRG 1 cut(s) 355
EcoO109I RGGNCCY 2 cut(s) 53, 646
EcoRII CCWGG 1 cut(s) 601
EcoRV GATATC 1 cut(s) 624
EcoT14I CCWWGG 1 cut(s) 49
ErhI CCWWGG 1 cut(s) 49
Esp3I CGTCTC 1 cut(s) 507
FaeI CATG 3 cut(s) 238, 397, 551
FaiI YATR 4 cut(s) 236, 395, 549, 557
FaqI GGGAC 2 cut(s) 39, 659
FatI CATG 3 cut(s) 234, 393, 547
FauI CCCGC 2 cut(s) 65, 132
Fnu4HI GCNGC 6 cut(s) 113, 312, 433, 436, 494, 562
FokI GGATG 7 cut(s) 114, 121, 132, 156, 197, 595, 601
Fsp4HI GCNGC 6 cut(s) 113, 312, 433, 436, 494, 562
GluI GCNGC 6 cut(s) 113, 312, 433, 436, 494, 562
GsaI CCCAGC 2 cut(s) 11, 145
GsuI CTGGAG 1 cut(s) 6
HaeIII GGCC 4 cut(s) 16, 261, 300, 360
HapII CCGG 5 cut(s) 179, 258, 308, 476, 509
HgaI GACGC 1 cut(s) 489
Hin1I GRCGYC 1 cut(s) 552
Hin1II CATG 3 cut(s) 238, 397, 551
HincII GTYRAC 1 cut(s) 79
HindII GTYRAC 1 cut(s) 79
HinfI GANTC 2 cut(s) 86, 318
HpaII CCGG 5 cut(s) 179, 258, 308, 476, 509
HphI GGTGA 1 cut(s) 196
Hpy166II GTNNAC 2 cut(s) 79, 331
Hpy188III TCNNGA 4 cut(s) 94, 100, 394, 416
Hpy8I GTNNAC 2 cut(s) 79, 331
HpyAV CCTTC 3 cut(s) 72, 273, 282
HpyCH4IV ACGT 1 cut(s) 552
HpyCH4V TGCA 3 cut(s) 314, 451, 667
HpyF10VI GCNNNNNNNGC 4 cut(s) 112, 258, 320, 544
HpyF3I CTNAG 2 cut(s) 193, 241
HpySE526I ACGT 1 cut(s) 552
Hsp92I GRCGYC 1 cut(s) 552
Hsp92II CATG 3 cut(s) 238, 397, 551
KroI GCCGGC 2 cut(s) 307, 508
KroNI GCCGGC 2 cut(s) 309, 510
Kzo9I GATC 3 cut(s) 96, 186, 421
Lsp1109I GCAGC 2 cut(s) 298, 573
LweI GCATC 4 cut(s) 136, 143, 154, 547
MaeII ACGT 1 cut(s) 552
MaeIII GTNAC 2 cut(s) 373, 514
MalI GATC 3 cut(s) 98, 188, 423
MboI GATC 3 cut(s) 96, 186, 421
MboII GAAGA 3 cut(s) 431, 520, 603
MluCI AATT 3 cut(s) 346, 577, 658
MlyI GAGTC 2 cut(s) 80, 312
MmeI TCCRAC 1 cut(s) 271
MroNI GCCGGC 2 cut(s) 307, 508
MseI TTAA 2 cut(s) 654, 676
MslI CAYNNNNRTG 1 cut(s) 546
MspI CCGG 5 cut(s) 179, 258, 308, 476, 509
MspR9I CCNGG 2 cut(s) 477, 603
MvaI CCWGG 1 cut(s) 603
MwoI GCNNNNNNNGC 4 cut(s) 112, 258, 320, 544
NaeI GCCGGC 2 cut(s) 309, 510
NciI CCSGG 1 cut(s) 477
NdeII GATC 3 cut(s) 96, 186, 421
NgoMIV GCCGGC 2 cut(s) 307, 508
NlaIII CATG 3 cut(s) 238, 397, 551
NlaIV GGNNCC 2 cut(s) 55, 647
NmuCI GTSAC 1 cut(s) 373
PagI TCATGA 1 cut(s) 393
PceI AGGCCT 1 cut(s) 16
PdiI GCCGGC 2 cut(s) 309, 510
PflFI GACNNNGTC 1 cut(s) 89
PfoI TCCNGGA 1 cut(s) 475
PkrI GCNGC 6 cut(s) 114, 313, 434, 437, 495, 563
PleI GAGTC 2 cut(s) 80, 312
PpsI GAGTC 2 cut(s) 80, 312
PpuMI RGGWCCY 2 cut(s) 53, 646
Psp5II RGGWCCY 2 cut(s) 53, 646
Psp6I CCWGG 1 cut(s) 601
PspFI CCCAGC 2 cut(s) 7, 141
PspGI CCWGG 1 cut(s) 601
PspN4I GGNNCC 2 cut(s) 55, 647
PspPI GGNCC 5 cut(s) 53, 260, 358, 605, 646
PspPPI RGGWCCY 2 cut(s) 53, 646
PsyI GACNNNGTC 1 cut(s) 89
RsaI GTAC 1 cut(s) 330
RsaNI GTAC 1 cut(s) 329
RseI CAYNNNNRTG 1 cut(s) 546
SaqAI TTAA 2 cut(s) 654, 676
SatI GCNGC 6 cut(s) 113, 312, 433, 436, 494, 562
Sau3AI GATC 3 cut(s) 96, 186, 421
Sau96I GGNCC 5 cut(s) 53, 260, 358, 605, 646
SchI GAGTC 2 cut(s) 80, 312
ScrFI CCNGG 2 cut(s) 477, 603
SetI ASST 8 cut(s) 55, 154, 160, 166, 356, 555, 619, 651
SfaNI GCATC 4 cut(s) 136, 143, 154, 547
SinI GGWCC 3 cut(s) 53, 605, 646
SmiMI CAYNNNNRTG 1 cut(s) 546
Sse9I AATT 3 cut(s) 346, 577, 658
SseBI AGGCCT 1 cut(s) 16
SspI AATATT 1 cut(s) 483
StuI AGGCCT 1 cut(s) 16
StyD4I CCNGG 2 cut(s) 475, 601
StyI CCWWGG 1 cut(s) 49
TaiI ACGT 1 cut(s) 555
TaqI TCGA 1 cut(s) 572
TasI AATT 3 cut(s) 346, 577, 658
TauI GCSGC 4 cut(s) 115, 435, 438, 496
Tru1I TTAA 2 cut(s) 654, 676
Tru9I TTAA 2 cut(s) 654, 676
TscAI CASTG 4 cut(s) 201, 226, 385, 453
TseFI GTSAC 1 cut(s) 373
TseI GCWGC 2 cut(s) 311, 561
Tsp45I GTSAC 1 cut(s) 373
TspDTI ATGAA 4 cut(s) 17, 223, 382, 474
TspGWI ACGGA 3 cut(s) 79, 316, 597
TspRI CASTG 4 cut(s) 201, 226, 385, 453
Tth111I GACNNNGTC 1 cut(s) 89
VpaK11BI GGWCC 3 cut(s) 53, 605, 646
ZraI GACGTC 1 cut(s) 553
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.