pycom05g01090

VQ motif

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0003162
Physical Location & Seq
Forward (+)
1320531 .. 1321190
660 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g01090.1

Sequence Viewer

Length: 660 bp
ATGGATTATTCAACAGGAAAATCTCCAAGAAGAGAGCTTCAACTCCAAGGTCCACGTCCAACGCCTCTCAGGATACACAAAGACTCTCACAAGATCAAGAAACCTCCAGTTGTACCACAACCGTCACAACAACCAAACCAGCAGCAGCAGCCCCATCACTACCAGCAGCCACGTCAGCCGGTCATAATCTACACGGTGTCCCCGAAGGTCATCCACACAAACCCTAGCGAGTTCATGGACCTTGTCCAACGCCTCACCGGCTTACCACGATCAAATTCATCAACTACATCGTCTTCACCGCCTAGTAATAACAATAGTAATCCTCTGAATCACGATAACTATAATAACCCTCCAGCTCATGACGATCAAGGGATGAAGAAAAACAGTACTGCTCAAGATGTTGTGGAAGAAGTGGAAATAATGGATGACGGTGGTGTGAAAAAGGGTTTGTTTCCGGGGATCTTGTCACCTGGGCCCGCTTCGCTTACTCCAATTCCGTCAAACTTCTTCTCTCCGCCGGCAGATAGCTTCTTTCATGATTTGAGCCCCGTGCTTTATGGCAACAGGAACTTCATAGAAGGTAGCTTCATGCCTAGTCCTTCAAACTTCTTTTCCCCTTCTACATCCATAGACTTATTCAACAATTTTCCATATTTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04016 map04016
Pfam Domains
Protein Families
VQ

Protein Analysis

220

Amino Acids

24.37

Weight (kDa)

7.11

Isoelectric Point (pI)

73.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VQ PF05678 65 - 89 1.3e-12 VQ motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 299, 477, 515
AclWI GGATC 1 cut(s) 467
AcsI RAATTY 1 cut(s) 274
AfaI GTAC 2 cut(s) 114, 388
AgsI TTSAA 4 cut(s) 12, 41, 603, 640
AjiI CACGTC 2 cut(s) 56, 173
AjnI CCWGG 1 cut(s) 469
AluBI AGCT 4 cut(s) 37, 356, 528, 585
AluI AGCT 4 cut(s) 37, 356, 528, 585
AlwI GGATC 1 cut(s) 467
AoxI GGCC 1 cut(s) 473
ApaI GGGCCC 1 cut(s) 477
ApeKI GCWGC 4 cut(s) 142, 145, 148, 166
ApoI RAATTY 1 cut(s) 274
AspS9I GGNCC 4 cut(s) 50, 238, 473, 474
AsuC2I CCSGG 1 cut(s) 456
AsuHPI GGTGA 3 cut(s) 247, 288, 459
AvaII GGWCC 2 cut(s) 50, 238
BaeGI GKGCMC 1 cut(s) 477
BanII GRGCYC 2 cut(s) 477, 548
BbsI GAAGAC 1 cut(s) 285
BbvI GCAGC 4 cut(s) 154, 157, 160, 178
BccI CCATC 1 cut(s) 162
BciT130I CCWGG 1 cut(s) 471
BciVI GTATCC 1 cut(s) 66
BcnI CCSGG 1 cut(s) 456
BfaI CTAG 3 cut(s) 225, 303, 594
BfuI GTATCC 1 cut(s) 66
BglI GCCNNNNNGGC 1 cut(s) 258
BisI GCNGC 4 cut(s) 143, 146, 149, 167
BlsI GCNGC 4 cut(s) 144, 147, 150, 168
BmcAI AGTACT 1 cut(s) 388
Bme1390I CCNGG 2 cut(s) 456, 471
Bme18I GGWCC 2 cut(s) 50, 238
BmgBI CACGTC 2 cut(s) 56, 173
BmgT120I GGNCC 4 cut(s) 50, 238, 473, 474
BmiI GGNNCC 1 cut(s) 475
BmrFI CCNGG 2 cut(s) 456, 471
BpiI GAAGAC 1 cut(s) 285
BpmI CTGGAG 2 cut(s) 90, 336
BpuEI CTTGAG 1 cut(s) 378
BpuMI CCSGG 1 cut(s) 456
BsaJI CCNNGG 3 cut(s) 46, 455, 470
Bse118I RCCGGY 3 cut(s) 178, 257, 517
Bse1I ACTGG 1 cut(s) 107
BseBI CCWGG 1 cut(s) 471
BseDI CCNNGG 3 cut(s) 46, 455, 470
BseGI GGATG 4 cut(s) 210, 378, 430, 623
BseMII CTCAG 1 cut(s) 82
BseNI ACTGG 1 cut(s) 107
BseSI GKGCMC 1 cut(s) 477
BseXI GCAGC 4 cut(s) 154, 157, 160, 178
BshFI GGCC 1 cut(s) 475
BsiSI CCGG 4 cut(s) 179, 258, 455, 518
BslFI GGGAC 1 cut(s) 184
BsmFI GGGAC 1 cut(s) 184
BsnI GGCC 1 cut(s) 475
Bsp120I GGGCCC 1 cut(s) 473
Bsp1286I GDGCHC 2 cut(s) 477, 548
Bsp143I GATC 4 cut(s) 93, 269, 364, 459
BspACI CCGC 3 cut(s) 299, 477, 515
BspANI GGCC 1 cut(s) 475
BspCNI CTCAG 1 cut(s) 81
BspHI TCATGA 2 cut(s) 358, 535
BspLI GGNNCC 1 cut(s) 475
BspPI GGATC 1 cut(s) 467
BsrFI RCCGGY 3 cut(s) 178, 257, 517
BsrI ACTGG 1 cut(s) 107
BssAI RCCGGY 3 cut(s) 178, 257, 517
BssECI CCNNGG 3 cut(s) 46, 455, 470
BssMI GATC 4 cut(s) 93, 269, 364, 459
BssT1I CCWWGG 1 cut(s) 46
Bst2UI CCWGG 1 cut(s) 471
Bst4CI ACNGT 4 cut(s) 123, 196, 386, 431
Bst6I CTCTTC 1 cut(s) 25
BstC8I GCNNGC 2 cut(s) 477, 519
BstDEI CTNAG 1 cut(s) 68
BstF5I GGATG 4 cut(s) 210, 378, 430, 623
BstKTI GATC 4 cut(s) 96, 272, 367, 462
BstMBI GATC 4 cut(s) 93, 269, 364, 459
BstMWI GCNNNNNNNGC 4 cut(s) 148, 175, 258, 481
BstNI CCWGG 1 cut(s) 471
BstSCI CCNGG 2 cut(s) 454, 469
BstSLI GKGCMC 1 cut(s) 477
BstV1I GCAGC 4 cut(s) 154, 157, 160, 178
BstV2I GAAGAC 1 cut(s) 285
BstX2I RGATCY 1 cut(s) 459
BstYI RGATCY 1 cut(s) 459
BsuI GTATCC 1 cut(s) 66
BsuRI GGCC 1 cut(s) 475
BtrI CACGTC 2 cut(s) 56, 173
BtsCI GGATG 4 cut(s) 210, 378, 430, 623
Cac8I GCNNGC 2 cut(s) 477, 519
CciI TCATGA 2 cut(s) 358, 535
Cfr10I RCCGGY 3 cut(s) 178, 257, 517
Cfr13I GGNCC 4 cut(s) 50, 238, 473, 474
Csp6I GTAC 2 cut(s) 113, 387
CviAII CATG 4 cut(s) 235, 359, 536, 589
CviQI GTAC 2 cut(s) 113, 387
DdeI CTNAG 1 cut(s) 68
DpnI GATC 4 cut(s) 95, 271, 366, 461
DpnII GATC 4 cut(s) 93, 269, 364, 459
Eam1104I CTCTTC 1 cut(s) 25
EarI CTCTTC 1 cut(s) 25
EciI GGCGGA 1 cut(s) 504
Eco130I CCWWGG 1 cut(s) 46
Eco24I GRGCYC 2 cut(s) 477, 548
Eco47I GGWCC 2 cut(s) 50, 238
EcoRII CCWGG 1 cut(s) 469
EcoT14I CCWWGG 1 cut(s) 46
EcoT38I GRGCYC 2 cut(s) 477, 548
ErhI CCWWGG 1 cut(s) 46
FaeI CATG 4 cut(s) 238, 362, 539, 592
FaqI GGGAC 1 cut(s) 184
FatI CATG 4 cut(s) 234, 358, 535, 588
FauI CCCGC 1 cut(s) 484
Fnu4HI GCNGC 4 cut(s) 143, 146, 149, 167
FokI GGATG 4 cut(s) 197, 385, 437, 610
FriOI GRGCYC 2 cut(s) 477, 548
Fsp4HI GCNGC 4 cut(s) 143, 146, 149, 167
FspBI CTAG 3 cut(s) 225, 303, 594
GluI GCNGC 4 cut(s) 143, 146, 149, 167
GsuI CTGGAG 2 cut(s) 90, 336
HaeIII GGCC 1 cut(s) 475
HapII CCGG 4 cut(s) 179, 258, 455, 518
Hin1II CATG 4 cut(s) 238, 362, 539, 592
HinfI GANTC 2 cut(s) 83, 328
HpaII CCGG 4 cut(s) 179, 258, 455, 518
HphI GGTGA 3 cut(s) 247, 288, 459
Hpy166II GTNNAC 1 cut(s) 53
Hpy188I TCNGA 1 cut(s) 327
Hpy188III TCNNGA 6 cut(s) 70, 97, 332, 359, 395, 536
Hpy8I GTNNAC 1 cut(s) 53
HpyAV CCTTC 4 cut(s) 199, 572, 609, 627
HpyCH4III ACNGT 4 cut(s) 123, 196, 386, 431
HpyCH4IV ACGT 2 cut(s) 55, 172
HpyF10VI GCNNNNNNNGC 4 cut(s) 148, 175, 258, 481
HpyF3I CTNAG 1 cut(s) 68
HpySE526I ACGT 2 cut(s) 55, 172
Hsp92II CATG 4 cut(s) 238, 362, 539, 592
KroI GCCGGC 1 cut(s) 517
KroNI GCCGGC 1 cut(s) 519
Kzo9I GATC 4 cut(s) 93, 269, 364, 459
Lsp1109I GCAGC 4 cut(s) 154, 157, 160, 178
MaeI CTAG 3 cut(s) 225, 303, 594
MaeII ACGT 2 cut(s) 55, 172
MaeIII GTNAC 2 cut(s) 123, 465
MalI GATC 4 cut(s) 95, 271, 366, 461
MboI GATC 4 cut(s) 93, 269, 364, 459
MboII GAAGA 5 cut(s) 42, 285, 388, 419, 499
MflI RGATCY 1 cut(s) 459
MhlI GDGCHC 2 cut(s) 477, 548
MluCI AATT 3 cut(s) 274, 492, 643
MlyI GAGTC 1 cut(s) 77
MmeI TCCRAC 2 cut(s) 83, 271
MnlI CCTC 5 cut(s) 75, 114, 263, 333, 360
MroNI GCCGGC 1 cut(s) 517
MspI CCGG 4 cut(s) 179, 258, 455, 518
MspR9I CCNGG 2 cut(s) 456, 471
MvaI CCWGG 1 cut(s) 471
MwoI GCNNNNNNNGC 4 cut(s) 148, 175, 258, 481
NaeI GCCGGC 1 cut(s) 519
NciI CCSGG 1 cut(s) 456
NdeII GATC 4 cut(s) 93, 269, 364, 459
NgoMIV GCCGGC 1 cut(s) 517
NlaIII CATG 4 cut(s) 238, 362, 539, 592
NlaIV GGNNCC 1 cut(s) 475
NmuCI GTSAC 2 cut(s) 123, 465
PagI TCATGA 2 cut(s) 358, 535
PcsI WCGNNNNNNNCGW 1 cut(s) 200
PdiI GCCGGC 1 cut(s) 519
PfeI GAWTC 1 cut(s) 328
PflFI GACNNNGTC 1 cut(s) 242
PkrI GCNGC 4 cut(s) 144, 147, 150, 168
PleI GAGTC 1 cut(s) 77
PpsI GAGTC 1 cut(s) 77
Psp6I CCWGG 1 cut(s) 469
PspGI CCWGG 1 cut(s) 469
PspN4I GGNNCC 1 cut(s) 475
PspOMI GGGCCC 1 cut(s) 473
PspPI GGNCC 4 cut(s) 50, 238, 473, 474
PsuI RGATCY 1 cut(s) 459
PsyI GACNNNGTC 1 cut(s) 242
RsaI GTAC 2 cut(s) 114, 388
RsaNI GTAC 2 cut(s) 113, 387
SatI GCNGC 4 cut(s) 143, 146, 149, 167
Sau3AI GATC 4 cut(s) 93, 269, 364, 459
Sau96I GGNCC 4 cut(s) 50, 238, 473, 474
ScaI AGTACT 1 cut(s) 388
SchI GAGTC 1 cut(s) 77
ScrFI CCNGG 2 cut(s) 456, 471
SduI GDGCHC 2 cut(s) 477, 548
SinI GGWCC 2 cut(s) 50, 238
SmlI CTYRAG 1 cut(s) 393
SmoI CTYRAG 1 cut(s) 393
Sse9I AATT 3 cut(s) 274, 492, 643
SsiI CCGC 3 cut(s) 299, 477, 515
SspMI CTAG 3 cut(s) 225, 303, 594
StyD4I CCNGG 2 cut(s) 454, 469
StyI CCWWGG 1 cut(s) 46
TaaI ACNGT 4 cut(s) 123, 196, 386, 431
TaiI ACGT 2 cut(s) 58, 175
TasI AATT 3 cut(s) 274, 492, 643
TatI WGTACW 1 cut(s) 386
TfiI GAWTC 1 cut(s) 328
TseFI GTSAC 2 cut(s) 123, 465
TseI GCWGC 4 cut(s) 142, 145, 148, 166
Tsp45I GTSAC 2 cut(s) 123, 465
TspDTI ATGAA 6 cut(s) 223, 267, 389, 524, 562, 577
TspGWI ACGGA 1 cut(s) 486
Tth111I GACNNNGTC 1 cut(s) 242
VpaK11BI GGWCC 2 cut(s) 50, 238
XapI RAATTY 1 cut(s) 274
XspI CTAG 3 cut(s) 225, 303, 594
ZrmI AGTACT 1 cut(s) 388
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.