Rroxscaffold_2G00123640

VQ motif

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0003162
Physical Location & Seq
Forward (+)
57532497 .. 57533067
571 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00123640.1

Sequence Viewer

Length: 480 bp
ATGAACCCGCCACCGCAGCGGCCCACAACCCCCAAAAAGGAGCTCCAAATCCAAGGCCCCCGCCCCTCCCCTCTCAGACTCAGCAAAGACTCCGTCAAAATCAAGAAGCCGCCTCCCCACCCCCACCTCCGCCAAAACCCACCTCAACAACAGTTCCAGCCGCCGCAGCAGCACCAGGAGCCGGTGGTCATCTACACCGTCTCGCCCAAAGTGATTCACACCTCCGTCAACGAGTTCATGTCCCTCGTCCAACGCCTCACCGGCCCTTCCTCCTCTCCCCCGGCGACGTCTCCCCCGCCGCCCGCCTCGCCTCCATCGAGAAAACCAGCCCCTCTTCCGATCACAAGCATTCCCGGGGATTCTGTCGCCGGCGCCGGCCACCATGCCGCCGATCCCAGCTGGGTTCTTCTCTCCGGCCGCCGAAGACCCGCAAAACTTCAACTGGCAAGGCGCCAACAACATCAACAGCGGCTTCAATAG

Protein Analysis

159

Amino Acids

17.51

Weight (kDa)

11.73

Isoelectric Point (pI)

90.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VQ PF05678 66 - 93 8.4e-13 VQ motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 290
AccB1I GGYRCC 2 cut(s) 371, 450
AclWI GGATC 1 cut(s) 386
AcoI YGGCCR 2 cut(s) 376, 415
AcyI GRCGYC 3 cut(s) 287, 372, 451
AfiI CCNNNNNNNGG 2 cut(s) 37, 181
AgsI TTSAA 2 cut(s) 440, 476
AjnI CCWGG 1 cut(s) 174
AluBI AGCT 2 cut(s) 43, 399
AluI AGCT 2 cut(s) 43, 399
Alw21I GWGCWC 1 cut(s) 45
Alw26I GTCTC 2 cut(s) 205, 294
AlwI GGATC 1 cut(s) 386
Ama87I CYCGRG 1 cut(s) 353
AoxI GGCC 5 cut(s) 20, 55, 262, 376, 415
ApeKI GCWGC 3 cut(s) 16, 166, 169
ArsI GACNNNNNNTTYG 2 cut(s) 78, 110
AspLEI GCGC 2 cut(s) 374, 453
AspS9I GGNCC 3 cut(s) 21, 56, 263
AsuC2I CCSGG 3 cut(s) 281, 354, 355
AsuHPI GGTGA 1 cut(s) 250
AvaI CYCGRG 1 cut(s) 353
BanI GGYRCC 2 cut(s) 371, 450
BanII GRGCYC 1 cut(s) 45
BbsI GAAGAC 1 cut(s) 430
Bbv12I GWGCWC 1 cut(s) 45
BbvI GCAGC 3 cut(s) 28, 178, 181
BccI CCATC 1 cut(s) 322
BciT130I CCWGG 1 cut(s) 176
BcnI CCSGG 3 cut(s) 281, 354, 355
BcoDI GTCTC 2 cut(s) 205, 294
BfoI RGCGCY 2 cut(s) 375, 454
BglI GCCNNNNNGGC 1 cut(s) 261
Bme1390I CCNGG 4 cut(s) 176, 281, 354, 355
BmeT110I CYCGRG 1 cut(s) 353
BmgT120I GGNCC 3 cut(s) 21, 56, 263
BmiI GGNNCC 4 cut(s) 58, 180, 373, 452
BmrFI CCNGG 4 cut(s) 176, 281, 354, 355
BpiI GAAGAC 1 cut(s) 430
BpuMI CCSGG 3 cut(s) 281, 354, 355
BsaHI GRCGYC 3 cut(s) 287, 372, 451
BsaJI CCNNGG 4 cut(s) 52, 279, 353, 354
Bsc4I CCNNNNNNNGG 2 cut(s) 37, 181
Bse118I RCCGGY 4 cut(s) 181, 260, 368, 374
Bse1I ACTGG 1 cut(s) 447
BseBI CCWGG 1 cut(s) 176
BseDI CCNNGG 4 cut(s) 52, 279, 353, 354
BseLI CCNNNNNNNGG 2 cut(s) 37, 181
BseMII CTCAG 2 cut(s) 88, 94
BseNI ACTGG 1 cut(s) 447
BseRI GAGGAG 1 cut(s) 262
BseX3I CGGCCG 1 cut(s) 415
BseXI GCAGC 3 cut(s) 28, 178, 181
BseYI CCCAGC 2 cut(s) 395, 399
Bsh1285I CGRYCG 1 cut(s) 418
BshFI GGCC 5 cut(s) 22, 57, 264, 378, 417
BshNI GGYRCC 2 cut(s) 371, 450
BsiEI CGRYCG 1 cut(s) 418
BsiHKAI GWGCWC 1 cut(s) 45
BsiHKCI CYCGRG 1 cut(s) 353
BsiSI CCGG 7 cut(s) 182, 261, 281, 354, 369, 375, 414
BslFI GGGAC 1 cut(s) 226
BslI CCNNNNNNNGG 2 cut(s) 37, 181
BsmAI GTCTC 2 cut(s) 205, 294
BsmBI CGTCTC 2 cut(s) 205, 294
BsmFI GGGAC 1 cut(s) 226
BsmI GAATGC 1 cut(s) 348
BsnI GGCC 5 cut(s) 22, 57, 264, 378, 417
BsoBI CYCGRG 1 cut(s) 353
Bsp1286I GDGCHC 1 cut(s) 45
Bsp143I GATC 2 cut(s) 339, 391
BspANI GGCC 5 cut(s) 22, 57, 264, 378, 417
BspCNI CTCAG 2 cut(s) 87, 93
BspLI GGNNCC 4 cut(s) 58, 180, 373, 452
BspPI GGATC 1 cut(s) 386
BspT107I GGYRCC 2 cut(s) 371, 450
BsrFI RCCGGY 4 cut(s) 181, 260, 368, 374
BsrI ACTGG 1 cut(s) 447
BssAI RCCGGY 4 cut(s) 181, 260, 368, 374
BssECI CCNNGG 4 cut(s) 52, 279, 353, 354
BssMI GATC 2 cut(s) 339, 391
BssNI GRCGYC 3 cut(s) 287, 372, 451
BssT1I CCWWGG 1 cut(s) 52
Bst2UI CCWGG 1 cut(s) 176
Bst4CI ACNGT 2 cut(s) 153, 199
Bst6I CTCTTC 1 cut(s) 339
BstACI GRCGYC 3 cut(s) 287, 372, 451
BstC8I GCNNGC 3 cut(s) 303, 370, 376
BstDEI CTNAG 2 cut(s) 74, 80
BstH2I RGCGCY 2 cut(s) 375, 454
BstHHI GCGC 2 cut(s) 374, 453
BstKTI GATC 2 cut(s) 342, 394
BstMAI GTCTC 2 cut(s) 205, 294
BstMBI GATC 2 cut(s) 339, 391
BstMCI CGRYCG 1 cut(s) 418
BstMWI GCNNNNNNNGC 6 cut(s) 16, 166, 169, 178, 261, 307
BstNI CCWGG 1 cut(s) 176
BstSCI CCNGG 4 cut(s) 174, 279, 352, 353
BstV1I GCAGC 3 cut(s) 28, 178, 181
BstV2I GAAGAC 1 cut(s) 430
BstZI CGGCCG 1 cut(s) 415
BsuRI GGCC 5 cut(s) 22, 57, 264, 378, 417
Cac8I GCNNGC 3 cut(s) 303, 370, 376
CfoI GCGC 2 cut(s) 374, 453
Cfr10I RCCGGY 4 cut(s) 181, 260, 368, 374
Cfr13I GGNCC 3 cut(s) 21, 56, 263
Cfr9I CCCGGG 1 cut(s) 353
CviAII CATG 2 cut(s) 238, 383
DdeI CTNAG 2 cut(s) 74, 80
DinI GGCGCC 2 cut(s) 373, 452
DpnI GATC 2 cut(s) 341, 393
DpnII GATC 2 cut(s) 339, 391
EaeI YGGCCR 2 cut(s) 376, 415
EagI CGGCCG 1 cut(s) 415
Eam1104I CTCTTC 1 cut(s) 339
EarI CTCTTC 1 cut(s) 339
EciI GGCGGA 1 cut(s) 119
Ecl136II GAGCTC 1 cut(s) 43
EclXI CGGCCG 1 cut(s) 415
Eco130I CCWWGG 1 cut(s) 52
Eco24I GRGCYC 1 cut(s) 45
Eco52I CGGCCG 1 cut(s) 415
Eco53kI GAGCTC 1 cut(s) 43
Eco88I CYCGRG 1 cut(s) 353
EcoICRI GAGCTC 1 cut(s) 43
EcoO109I RGGNCCY 1 cut(s) 56
EcoRII CCWGG 1 cut(s) 174
EcoT14I CCWWGG 1 cut(s) 52
EcoT38I GRGCYC 1 cut(s) 45
EgeI GGCGCC 2 cut(s) 373, 452
EheI GGCGCC 2 cut(s) 373, 452
ErhI CCWWGG 1 cut(s) 52
Esp3I CGTCTC 2 cut(s) 205, 294
FaeI CATG 2 cut(s) 241, 386
FaiI YATR 2 cut(s) 239, 384
FaqI GGGAC 1 cut(s) 226
FatI CATG 2 cut(s) 237, 382
FauI CCCGC 5 cut(s) 15, 68, 303, 310, 436
FriOI GRGCYC 1 cut(s) 45
GlaI GCGC 2 cut(s) 373, 452
GsaI CCCAGC 2 cut(s) 399, 403
HaeII RGCGCY 2 cut(s) 375, 454
HaeIII GGCC 5 cut(s) 22, 57, 264, 378, 417
HapII CCGG 7 cut(s) 182, 261, 281, 354, 369, 375, 414
HhaI GCGC 2 cut(s) 374, 453
Hin1I GRCGYC 3 cut(s) 287, 372, 451
Hin1II CATG 2 cut(s) 241, 386
Hin6I GCGC 2 cut(s) 372, 451
HinP1I GCGC 2 cut(s) 372, 451
HincII GTYRAC 1 cut(s) 229
HindII GTYRAC 1 cut(s) 229
HinfI GANTC 4 cut(s) 78, 89, 214, 359
HpaII CCGG 7 cut(s) 182, 261, 281, 354, 369, 375, 414
HphI GGTGA 1 cut(s) 250
Hpy166II GTNNAC 1 cut(s) 229
Hpy188I TCNGA 2 cut(s) 77, 339
Hpy188III TCNNGA 2 cut(s) 103, 318
Hpy8I GTNNAC 1 cut(s) 229
Hpy99I CGWCG 1 cut(s) 289
HpyAV CCTTC 1 cut(s) 276
HpyCH4III ACNGT 2 cut(s) 153, 199
HpyCH4IV ACGT 1 cut(s) 287
HpyF10VI GCNNNNNNNGC 6 cut(s) 16, 166, 169, 178, 261, 307
HpyF3I CTNAG 2 cut(s) 74, 80
HpySE526I ACGT 1 cut(s) 287
Hsp92I GRCGYC 3 cut(s) 287, 372, 451
Hsp92II CATG 2 cut(s) 241, 386
HspAI GCGC 2 cut(s) 372, 451
KasI GGCGCC 2 cut(s) 371, 450
KroI GCCGGC 2 cut(s) 368, 374
KroNI GCCGGC 2 cut(s) 370, 376
Kzo9I GATC 2 cut(s) 339, 391
LmnI GCTCC 3 cut(s) 40, 48, 178
Lsp1109I GCAGC 3 cut(s) 28, 178, 181
MaeII ACGT 1 cut(s) 287
MalI GATC 2 cut(s) 341, 393
MboI GATC 2 cut(s) 339, 391
MboII GAAGA 3 cut(s) 326, 398, 435
MhlI GDGCHC 1 cut(s) 45
Mly113I GGCGCC 2 cut(s) 372, 451
MlyI GAGTC 2 cut(s) 72, 83
MmeI TCCRAC 1 cut(s) 274
MreI CGCCGGCG 1 cut(s) 368
MroNI GCCGGC 2 cut(s) 368, 374
MspA1I CMGCKG 3 cut(s) 19, 399, 469
MspI CCGG 7 cut(s) 182, 261, 281, 354, 369, 375, 414
MspR9I CCNGG 4 cut(s) 176, 281, 354, 355
Mva1269I GAATGC 1 cut(s) 348
MvaI CCWGG 1 cut(s) 176
MwoI GCNNNNNNNGC 6 cut(s) 16, 166, 169, 178, 261, 307
NaeI GCCGGC 2 cut(s) 370, 376
NarI GGCGCC 2 cut(s) 372, 451
NciI CCSGG 3 cut(s) 281, 354, 355
NdeII GATC 2 cut(s) 339, 391
NgoMIV GCCGGC 2 cut(s) 368, 374
NlaIII CATG 2 cut(s) 241, 386
NlaIV GGNNCC 4 cut(s) 58, 180, 373, 452
PcsI WCGNNNNNNNCGW 1 cut(s) 314
PctI GAATGC 1 cut(s) 348
PdiI GCCGGC 2 cut(s) 370, 376
PfeI GAWTC 2 cut(s) 214, 359
PflFI GACNNNGTC 1 cut(s) 92
PleI GAGTC 2 cut(s) 72, 83
PluTI GGCGCC 2 cut(s) 375, 454
PpsI GAGTC 2 cut(s) 72, 83
Psp124BI GAGCTC 1 cut(s) 45
Psp6I CCWGG 1 cut(s) 174
PspFI CCCAGC 2 cut(s) 395, 399
PspGI CCWGG 1 cut(s) 174
PspN4I GGNNCC 4 cut(s) 58, 180, 373, 452
PspPI GGNCC 3 cut(s) 21, 56, 263
PsyI GACNNNGTC 1 cut(s) 92
PvuII CAGCTG 1 cut(s) 399
SacI GAGCTC 1 cut(s) 45
Sau3AI GATC 2 cut(s) 339, 391
Sau96I GGNCC 3 cut(s) 21, 56, 263
SchI GAGTC 2 cut(s) 72, 83
ScrFI CCNGG 4 cut(s) 176, 281, 354, 355
SduI GDGCHC 1 cut(s) 45
SetI ASST 6 cut(s) 45, 129, 145, 224, 290, 401
SfoI GGCGCC 2 cut(s) 373, 452
SgrAI CRCCGGYG 1 cut(s) 368
SmaI CCCGGG 1 cut(s) 355
SspDI GGCGCC 2 cut(s) 371, 450
SstI GAGCTC 1 cut(s) 45
StyD4I CCNGG 4 cut(s) 174, 279, 352, 353
StyI CCWWGG 1 cut(s) 52
TaaI ACNGT 2 cut(s) 153, 199
TaiI ACGT 1 cut(s) 290
TaqI TCGA 1 cut(s) 317
TauI GCSGC 8 cut(s) 22, 112, 163, 166, 301, 389, 420, 472
TfiI GAWTC 2 cut(s) 214, 359
TseI GCWGC 3 cut(s) 16, 166, 169
TspDTI ATGAA 2 cut(s) 17, 226
TspGWI ACGGA 2 cut(s) 82, 214
TspMI CCCGGG 1 cut(s) 353
Tth111I GACNNNGTC 1 cut(s) 92
XmaI CCCGGG 1 cut(s) 353
ZraI GACGTC 1 cut(s) 288
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.