Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb1

Orthogroup ID

OG0003007

Physical Location & Seq

Genomic Coordinates Reverse (-)

18859064 .. 18863014

Gene Structure 3951 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_1g27100.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2238 bp

ATGTCGAAGCGAAAGTTCGGATTCGAAGGGTTCGGCATAAACCGGCAATCGACCTACAACTTCGAGCAGTCCCAGCAACCCCCTCAGCGCCTCTACGTCCCTCCCTCCTCCCGCGCCCACGGCCACGACAACTACGAAGACACCGACCTTGACAACATCGATTACGACGACGTCGACGCCGCCGCGAATAAAGGCGGAGACGGCGGTGAGGAAGAAGAAGTCGATCCGCTCGATGCGTTCATGGAGGGGATTCACGAGGAGGTGAGGTCAGCTCCGCCGCCGAAGCCGAAAGAGAAGGCGGACAAGTACCAGGACGACGACGAGGAGGATCATCTGGAGAGCTTCTTGAGGGCGAAGAAGGACGCCACGCTGACGCTGGCCTCCGACGCGCTCCACGCCGGATATGATTCCGACGAGGAGGTCTACGCCGCTGCCAAGGCTGTCGATGCCGGATTGGTGGAGTATGATTCGGATGACAATCCTGTTGTTGTCGATAGGAAGAGGATCGAGCCGATTTCGGCTCTGGATCATAGCTGCATTGACTACGAGCCTTTCACTAAGGATTTCTATGAGGAGAAAGAGTCCATTTCAGCGATGAGTGAGCAGGATGTTGCCGAGTACCGAAAGAGCTTGGCCATCCGAGTGTCTGGCTTTGATGTACCAAGGCCAGTGAAGACGTTTGACGACTGTGGGTTTTCTCCGCAACTCATGAATGCAATCAAAAAACAAGAGTATGAAAAGCCTACACCAATACAGTGCCAAGCTTTACCCATAGTTCTTTCAGGGAGGGATATCATTGCTATAGCGAAAACTGGTTCTGGAAAGACTGCTGCTTTTGTGCTTCCCATGATTTGCCATATTATGGATCAGCCGGAACTTCAGAAAGATGAAGGTCCTATAGGAGTAATATGTGCACCTACTAGAGAACTGGCGCAGCAAATACACCTAGAGGCCAAGAAATTTGCTAAATCACATGGCATCCGTGTCTCTGCTGTTTATGGTGGGATGTCGAAACTTGATCAGTTCAAAGAACTAAAGGCAGGATGTGAGATAGTTGTAGCTACTCCTGGGAGATTGATAGACATGATTAAAATGAAGGCACTGACGATGCAAAGGGCAACTTACCTAGTGCTTGATGAGGCTGATCGGATGTTTGACCTTGGTTTTGAGCCTCAAATTAGGTCTATAGTTGGTCAGATTAGGCCAGACCGTCAAACGTTACTCTTTTCTGCTACAATGCCCCGAAAGGTTGAAACGTTGGCTAGGGAAATTCTCTCTGACCCTGTAAGAGTTACCGTGGGTGAGGTGGGAATGGCCAATGAGGACATTACTCAAGTTGTTCATGTACTTCCTTCTGATGCTGAGAAGTTGTCATGGCTTCTTGAAAGATTACCGGGTATGATTGATGATGGGGATGTATTAGTATTTGCTTCTAAAAAGGCTGGAGTGGATGAGATCGAAACACAGCTTTCTCAGAAAGGTTTTAAAGCTGCAGCCATTCATGGTGATAAGGACCAGGCTACTAGGGTGGATATTATGAACAAATTTAAATCTGGCACCTACCATGTTCTGATTGCAACTGATGTTGCTGCTCGTGGTCTTGATATCAAGTCAATTAAGTCAGTGGTAAACTTTGATATTGCAAAAGACATGGACATGCATGTTCATCGTATTGGTAGAACAGGTCGTGCAGGTGATAAAGATGGGACTGCATACACTCTTATCACACATAAAGAGGCACGGTTTGCTGGAGAATTGGTTAATAGCTTAGTTGGGGCTGGTCAGAATGTTCCAGTGGAGCTCATGGACCTTGCGATGAAGGATGGAAGATTCAGGTCTAAACGTGATTCAAGAAAAGGAGGTGGAAAGAAGGGCAAAGGGAGAGGCGGTGGTAGAGGCGGTGGCAGCGGTGGCAACAGTGGTCGAGGTGTGCGTGGGGTGGATTTTGGTTTGGGTATTGGATATAATCCGGAATCCAACAGTTCTCCATCACCTGCTGTTACCAGTCGATCTGCCGCAGTTACTTCTTTAAGAACAGGAATGATGTCTCAGTTTAAGAATAAGTTTGTTTCTGCTACATCAAACTCTCCAAGTCAGGGTTCTGGTAACAGTTTGAGTGCGCATGCCAACAAGAGGCCTGCATTACGAGGATTTGTAGCTGCTGGTTCCATTGGGGGAGGCATATATGCGCCTCAGACAACCAATACAGTTTCTCCGCCTCCTGCATCAGCGTTCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0006139 GO:0006725 GO:0006807 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0009987 GO:0010501 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0031974 GO:0031981 GO:0034641 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0070013 GO:0070035 GO:0071704 GO:0090304 GO:0140098 GO:1901360

KEGG Pathways

Metabolic & Signaling

ko03040 map03040

Pfam Domains

Protein Families

DEAD Helicase_C

Protein Analysis

746

Amino Acids

80.85

Weight (kDa)

5.72

Isoelectric Point (pI)

43.56

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DEAD	PF00270	249 - 419	4.5e-52	DEAD/DEAH box helicase
Helicase_C	PF00271	460 - 565	1.1e-27	Helicase conserved C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0003007)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G47330
fragaria_vesca	FvH4_1g27100 FvH4_1g27100 FvH4_1g27100
malus_domestica	MD00G1118300.v1.1 MD00G1118400.v1.1 MD09G1283400.v1.1 MD17G1274100.v1.1 MD17G1274800.v1.1 MD17G1275300.v1.1 MD17G1276500.v1.1
prunus_persica	Prupe.3G010900_v2.0.a1 Prupe.3G010900_v2.0.a1 Prupe.3G010900_v2.0.a1 Prupe.3G010900_v2.0.a1 Prupe.3G010900_v2.0.a1
pyrus_communis	pycom09g19130 pycom09g19140 pycom17g27210 pycom17g27330
rosa_chinensis	RchiOBHm_Chr3g0487421
rosa_laevigata	RLG00000023004
rosa_multiflora	Rmu_sc0008232.1_g000009 Rmu_sc0022533.1_g000001 Rmu_sc0031316.1_g000001
rosa_roxburghii	Rroxscaffold_6G00394790
rosa_rugosa	Rorug03G0234000
rosa_samantha	Rh2AG395100 Rh2CG381700 Rh3AG282000 Rh3BG317600 Rh3CG315700 Rh3DG313600 Rh4CG235900 Rh7AG337700 Rh7AG476200 Rh7CG355800
rosa_wichuraiana	Rw3G024970

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	2 cut(s)	1682, 2002
AasI	GACNNNNNNGTC	1 cut(s)	419
AatII	GACGTC	1 cut(s)	174
Acc16I	TGCGCA	1 cut(s)	2121
Acc36I	ACCTGC	2 cut(s)	1682, 2002
AccB1I	GGYRCC	1 cut(s)	1556
AccB7I	CCANNNNNTGG	1 cut(s)	862
AccBSI	CCGCTC	1 cut(s)	229
AccI	GTMKAC	2 cut(s)	174, 423
AccII	CGCG	3 cut(s)	114, 185, 389
AccIII	TCCGGA	1 cut(s)	1969
AclI	AACGTT	2 cut(s)	1217, 1256
AclWI	GGATC	5 cut(s)	218, 336, 512, 534, 873
AcoI	YGGCCR	3 cut(s)	121, 633, 1314
AcsI	RAATTY	3 cut(s)	959, 1269, 1544
AcuI	CTGAAG	1 cut(s)	863
AcyI	GRCGYC	3 cut(s)	171, 177, 363
AfaI	GTAC	4 cut(s)	308, 620, 660, 1347
AfiI	CCNNNNNNNGG	3 cut(s)	862, 2096, 2133
AgsI	TTSAA	4 cut(s)	1027, 1253, 1385, 1851
AjnI	CCWGG	3 cut(s)	309, 1066, 1515
AjuI	GAANNNNNNNTTGG	2 cut(s)	2083, 2115
Alw21I	GWGCWC	2 cut(s)	916, 1803
Alw26I	GTCTC	3 cut(s)	192, 991, 2052
Alw44I	GTGCAC	1 cut(s)	912
AlwI	GGATC	5 cut(s)	218, 336, 512, 534, 873
Aor13HI	TCCGGA	1 cut(s)	1969
AoxI	GGCC	8 cut(s)	121, 378, 633, 665, 951, 1202, 1314, 2135
ApaLI	GTGCAC	1 cut(s)	912
ApeKI	GCWGC	9 cut(s)	431, 534, 830, 934, 1490, 1493, 1589, 1905, 2159
ApoI	RAATTY	3 cut(s)	959, 1269, 1544
ArsI	GACNNNNNNTTYG	2 cut(s)	1148, 1180
AspLEI	GCGC	6 cut(s)	90, 116, 391, 934, 2122, 2191
AspS9I	GGNCC	3 cut(s)	893, 1513, 1807
AsuC2I	CCSGG	1 cut(s)	1395
AsuHPI	GGTGA	6 cut(s)	218, 274, 1313, 1517, 1706, 1983
AsuII	TTCGAA	1 cut(s)	24
AvaII	GGWCC	3 cut(s)	893, 1513, 1807
BaeGI	GKGCMC	1 cut(s)	916
BalI	TGGCCA	2 cut(s)	635, 1316
BanI	GGYRCC	1 cut(s)	1556
BanII	GRGCYC	1 cut(s)	1803
BauI	CACGAG	2 cut(s)	254, 1593
BbsI	GAAGAC	2 cut(s)	144, 680
Bbv12I	GWGCWC	2 cut(s)	916, 1803
BbvCI	CCTCAGC	1 cut(s)	84
BbvI	GCAGC	9 cut(s)	418, 521, 817, 946, 1477, 1505, 1576, 1917, 2146
BccI	CCATC	5 cut(s)	644, 1403, 1697, 1817, 1996
BceAI	ACGGC	2 cut(s)	136, 217
BcgI	CGANNNNNNTGC	2 cut(s)	1649, 1683
BciT130I	CCWGG	3 cut(s)	311, 1068, 1517
BclI	TGATCA	1 cut(s)	1018
BcnI	CCSGG	1 cut(s)	1395
BcoDI	GTCTC	3 cut(s)	192, 991, 2052
BfaI	CTAG	6 cut(s)	921, 947, 1127, 1263, 1524, 2236
BfmI	CTRYAG	4 cut(s)	801, 897, 1185, 1491
BfoI	RGCGCY	1 cut(s)	91
BfuAI	ACCTGC	2 cut(s)	1682, 2002
Bme1390I	CCNGG	4 cut(s)	311, 1068, 1395, 1517
Bme18I	GGWCC	3 cut(s)	893, 1513, 1807
BmgT120I	GGNCC	3 cut(s)	893, 1513, 1807
BmiI	GGNNCC	2 cut(s)	1558, 2167
BmrFI	CCNGG	4 cut(s)	311, 1068, 1395, 1517
BmsI	GCATC	6 cut(s)	223, 436, 987, 1098, 1348, 2234
BpiI	GAAGAC	2 cut(s)	144, 680
BplI	GAGNNNNNCTC	2 cut(s)	256, 288
BpmI	CTGGAG	3 cut(s)	356, 1464, 1770
Bpu10I	CCTNAGC	1 cut(s)	84
Bpu14I	TTCGAA	1 cut(s)	24
BpuEI	CTTGAG	2 cut(s)	367, 1317
BpuMI	CCSGG	1 cut(s)	1395
Bsa29I	ATCGAT	1 cut(s)	159
BsaBI	GATNNNNATC	1 cut(s)	477
BsaHI	GRCGYC	3 cut(s)	171, 177, 363
BsaJI	CCNNGG	6 cut(s)	118, 435, 662, 1067, 1159, 1296
BsaWI	WCCGGW	1 cut(s)	1969
Bsc4I	CCNNNNNNNGG	3 cut(s)	862, 2096, 2133
Bse118I	RCCGGY	1 cut(s)	42
Bse1I	ACTGG	5 cut(s)	668, 817, 933, 1793, 2004
Bse3DI	GCAATG	1 cut(s)	795
Bse8I	GATNNNNATC	1 cut(s)	477
BseAI	TCCGGA	1 cut(s)	1969
BseBI	CCWGG	3 cut(s)	311, 1068, 1517
BseCI	ATCGAT	1 cut(s)	159
BseDI	CCNNGG	6 cut(s)	118, 435, 662, 1067, 1159, 1296
BseJI	GATNNNNATC	1 cut(s)	477
BseLI	CCNNNNNNNGG	3 cut(s)	862, 2096, 2133
BseMI	GCAATG	1 cut(s)	795
BseMII	CTCAG	5 cut(s)	98, 1353, 1487, 2063, 2207
BseNI	ACTGG	5 cut(s)	668, 817, 933, 1793, 2004
BseRI	GAGGAG	5 cut(s)	97, 272, 338, 431, 587
BseSI	GKGCMC	1 cut(s)	916
BseXI	GCAGC	9 cut(s)	418, 521, 817, 946, 1477, 1505, 1576, 1917, 2146
BseYI	CCCAGC	1 cut(s)	72
BsgI	GTGCAG	1 cut(s)	1710
Bsh1236I	CGCG	3 cut(s)	114, 185, 389
BshFI	GGCC	8 cut(s)	123, 380, 635, 667, 953, 1204, 1316, 2137
BshNI	GGYRCC	1 cut(s)	1556
BshVI	ATCGAT	1 cut(s)	159
BsiHKAI	GWGCWC	2 cut(s)	916, 1803
BsiSI	CCGG	6 cut(s)	43, 399, 450, 872, 1394, 1970
BslFI	GGGAC	3 cut(s)	55, 83, 1720
BslI	CCNNNNNNNGG	3 cut(s)	862, 2096, 2133
BsmAI	GTCTC	3 cut(s)	192, 991, 2052
BsmBI	CGTCTC	1 cut(s)	192
BsmFI	GGGAC	3 cut(s)	55, 83, 1720
BsmI	GAATGC	1 cut(s)	718
BsnI	GGCC	8 cut(s)	123, 380, 635, 667, 953, 1204, 1316, 2137
Bsp119I	TTCGAA	1 cut(s)	24
Bsp1286I	GDGCHC	2 cut(s)	916, 1803
Bsp13I	TCCGGA	1 cut(s)	1969
Bsp143I	GATC	9 cut(s)	223, 328, 504, 526, 865, 1018, 1144, 1455, 2009
BspANI	GGCC	8 cut(s)	123, 380, 635, 667, 953, 1204, 1316, 2137
BspCNI	CTCAG	5 cut(s)	97, 1354, 1486, 2062, 2206
BspDI	ATCGAT	1 cut(s)	159
BspEI	TCCGGA	1 cut(s)	1969
BspFNI	CGCG	3 cut(s)	114, 185, 389
BspHI	TCATGA	1 cut(s)	708
BspLI	GGNNCC	2 cut(s)	1558, 2167
BspMAI	CTGCAG	1 cut(s)	1495
BspMI	ACCTGC	2 cut(s)	1682, 2002
BspPI	GGATC	5 cut(s)	218, 336, 512, 534, 873
BspT104I	TTCGAA	1 cut(s)	24
BspT107I	GGYRCC	1 cut(s)	1556
BsrBI	CCGCTC	1 cut(s)	229
BsrDI	GCAATG	1 cut(s)	795
BsrFI	RCCGGY	1 cut(s)	42
BsrI	ACTGG	5 cut(s)	668, 817, 933, 1793, 2004
BssAI	RCCGGY	1 cut(s)	42
BssECI	CCNNGG	6 cut(s)	118, 435, 662, 1067, 1159, 1296
BssMI	GATC	9 cut(s)	223, 328, 504, 526, 865, 1018, 1144, 1455, 2009
BssNI	GRCGYC	3 cut(s)	171, 177, 363
BssSI	CACGAG	2 cut(s)	254, 1593
BssT1I	CCWWGG	3 cut(s)	435, 662, 1159
Bst2BI	CACGAG	2 cut(s)	254, 1593
Bst2UI	CCWGG	3 cut(s)	311, 1068, 1517
Bst4CI	ACNGT	9 cut(s)	689, 756, 1211, 1297, 1743, 1919, 1982, 2111, 2209
Bst6I	CTCTTC	1 cut(s)	494
BstACI	GRCGYC	3 cut(s)	171, 177, 363
BstAPI	GCANNNNNTGC	1 cut(s)	1745
BstBI	TTCGAA	1 cut(s)	24
BstC8I	GCNNGC	3 cut(s)	378, 2124, 2139
BstDEI	CTNAG	7 cut(s)	84, 558, 1362, 1473, 1768, 2049, 2193
BstDSI	CCRYGG	2 cut(s)	118, 1296
BstFNI	CGCG	3 cut(s)	114, 185, 389
BstH2I	RGCGCY	1 cut(s)	91
BstHHI	GCGC	6 cut(s)	90, 116, 391, 934, 2122, 2191
BstKTI	GATC	9 cut(s)	226, 331, 507, 529, 868, 1021, 1147, 1458, 2012
BstMAI	GTCTC	3 cut(s)	192, 991, 2052
BstMBI	GATC	9 cut(s)	223, 328, 504, 526, 865, 1018, 1144, 1455, 2009
BstNI	CCWGG	3 cut(s)	311, 1068, 1517
BstNSI	RCATGY	3 cut(s)	1660, 1664, 2126
BstSCI	CCNGG	4 cut(s)	309, 1066, 1393, 1515
BstSFI	CTRYAG	4 cut(s)	801, 897, 1185, 1491
BstSLI	GKGCMC	1 cut(s)	916
BstUI	CGCG	3 cut(s)	114, 185, 389
BstV1I	GCAGC	9 cut(s)	418, 521, 817, 946, 1477, 1505, 1576, 1917, 2146
BstV2I	GAAGAC	2 cut(s)	144, 680
Bsu15I	ATCGAT	1 cut(s)	159
BsuRI	GGCC	8 cut(s)	123, 380, 635, 667, 953, 1204, 1316, 2137
BsuTUI	ATCGAT	1 cut(s)	159
BtgI	CCRYGG	2 cut(s)	118, 1296
BtgZI	GCGATG	2 cut(s)	608, 1829
BtsIMutI	CAGTG	6 cut(s)	675, 761, 1100, 1629, 1800, 1924
BveI	ACCTGC	2 cut(s)	1682, 2002
Cac8I	GCNNGC	3 cut(s)	378, 2124, 2139
CciI	TCATGA	1 cut(s)	708
CfoI	GCGC	6 cut(s)	90, 116, 391, 934, 2122, 2191
Cfr10I	RCCGGY	1 cut(s)	42
Cfr13I	GGNCC	3 cut(s)	893, 1513, 1807
ClaI	ATCGAT	1 cut(s)	159
CseI	GACGC	4 cut(s)	185, 371, 382, 395
Csp6I	GTAC	4 cut(s)	307, 619, 659, 1346
CviQI	GTAC	4 cut(s)	307, 619, 659, 1346
DdeI	CTNAG	7 cut(s)	84, 558, 1362, 1473, 1768, 2049, 2193
DpnI	GATC	9 cut(s)	225, 330, 506, 528, 867, 1020, 1146, 1457, 2011
DpnII	GATC	9 cut(s)	223, 328, 504, 526, 865, 1018, 1144, 1455, 2009
DraI	TTTAAA	2 cut(s)	1486, 1549
DrdI	GACNNNNNNGTC	1 cut(s)	419
DseDI	GACNNNNNNGTC	1 cut(s)	419
EaeI	YGGCCR	3 cut(s)	121, 633, 1314
Eam1104I	CTCTTC	1 cut(s)	494
EarI	CTCTTC	1 cut(s)	494
EciI	GGCGGA	4 cut(s)	210, 264, 314, 2205
Ecl136II	GAGCTC	1 cut(s)	1801
Eco130I	CCWWGG	3 cut(s)	435, 662, 1159
Eco147I	AGGCCT	1 cut(s)	2137
Eco24I	GRGCYC	1 cut(s)	1803
Eco32I	GATATC	2 cut(s)	793, 1606
Eco47I	GGWCC	3 cut(s)	893, 1513, 1807
Eco53kI	GAGCTC	1 cut(s)	1801
Eco57I	CTGAAG	1 cut(s)	863
EcoICRI	GAGCTC	1 cut(s)	1801
EcoO109I	RGGNCCY	1 cut(s)	893
EcoRII	CCWGG	3 cut(s)	309, 1066, 1515
EcoRV	GATATC	2 cut(s)	793, 1606
EcoT14I	CCWWGG	3 cut(s)	435, 662, 1159
EcoT22I	ATGCAT	1 cut(s)	1662
EcoT38I	GRGCYC	1 cut(s)	1803
ErhI	CCWWGG	3 cut(s)	435, 662, 1159
Esp3I	CGTCTC	1 cut(s)	192
FalI	AAGNNNNNCTT	2 cut(s)	1105, 1137
FaqI	GGGAC	3 cut(s)	55, 83, 1720
FauI	CCCGC	1 cut(s)	119
FbaI	TGATCA	1 cut(s)	1018
FblI	GTMKAC	2 cut(s)	174, 423
FriOI	GRGCYC	1 cut(s)	1803
FspAI	RTGCGCAY	1 cut(s)	2121
FspBI	CTAG	6 cut(s)	921, 947, 1127, 1263, 1524, 2236
FspI	TGCGCA	1 cut(s)	2121
GlaI	GCGC	6 cut(s)	89, 115, 390, 933, 2121, 2190
GsaI	CCCAGC	1 cut(s)	76
GsuI	CTGGAG	3 cut(s)	356, 1464, 1770
HaeII	RGCGCY	1 cut(s)	91
HaeIII	GGCC	8 cut(s)	123, 380, 635, 667, 953, 1204, 1316, 2137
HapII	CCGG	6 cut(s)	43, 399, 450, 872, 1394, 1970
HgaI	GACGC	4 cut(s)	185, 371, 382, 395
HhaI	GCGC	6 cut(s)	90, 116, 391, 934, 2122, 2191
Hin1I	GRCGYC	3 cut(s)	171, 177, 363
Hin6I	GCGC	6 cut(s)	88, 114, 389, 932, 2120, 2189
HinP1I	GCGC	6 cut(s)	88, 114, 389, 932, 2120, 2189
HincII	GTYRAC	1 cut(s)	175
HindII	GTYRAC	1 cut(s)	175
HindIII	AAGCTT	1 cut(s)	762
HinfI	GANTC	8 cut(s)	21, 250, 407, 467, 581, 1830, 1847, 1973
HpaII	CCGG	6 cut(s)	43, 399, 450, 872, 1394, 1970
HphI	GGTGA	6 cut(s)	218, 274, 1313, 1517, 1706, 1983
Hpy166II	GTNNAC	4 cut(s)	175, 424, 914, 1630
Hpy8I	GTNNAC	4 cut(s)	175, 424, 914, 1630
Hpy99I	CGWCG	8 cut(s)	170, 173, 176, 179, 320, 323, 389, 416
HpyAV	CCTTC	8 cut(s)	20, 289, 352, 884, 1090, 1362, 1813, 1864
HpyCH4III	ACNGT	9 cut(s)	689, 756, 1211, 1297, 1743, 1919, 1982, 2111, 2209
HpyCH4IV	ACGT	6 cut(s)	96, 171, 677, 1217, 1256, 1843
HpyF3I	CTNAG	7 cut(s)	84, 558, 1362, 1473, 1768, 2049, 2193
HpySE526I	ACGT	6 cut(s)	96, 171, 677, 1217, 1256, 1843
Hsp92I	GRCGYC	3 cut(s)	171, 177, 363
HspAI	GCGC	6 cut(s)	88, 114, 389, 932, 2120, 2189
Kpn2I	TCCGGA	1 cut(s)	1969
Ksp22I	TGATCA	1 cut(s)	1018
Kzo9I	GATC	9 cut(s)	223, 328, 504, 526, 865, 1018, 1144, 1455, 2009
LmnI	GCTCC	3 cut(s)	277, 396, 1798
Lsp1109I	GCAGC	9 cut(s)	418, 521, 817, 946, 1477, 1505, 1576, 1917, 2146
LweI	GCATC	6 cut(s)	223, 436, 987, 1098, 1348, 2234
MaeI	CTAG	6 cut(s)	921, 947, 1127, 1263, 1524, 2236
MaeII	ACGT	6 cut(s)	96, 171, 677, 1217, 1256, 1843
MaeIII	GTNAC	5 cut(s)	1218, 1291, 1999, 2020, 2105
MalI	GATC	9 cut(s)	225, 330, 506, 528, 867, 1020, 1146, 1457, 2011
MbiI	CCGCTC	1 cut(s)	229
MboI	GATC	9 cut(s)	223, 328, 504, 526, 865, 1018, 1144, 1455, 2009
MboII	GAAGA	7 cut(s)	149, 224, 227, 367, 511, 685, 1839
MhlI	GDGCHC	2 cut(s)	916, 1803
MlsI	TGGCCA	2 cut(s)	635, 1316
MluCI	AATT	6 cut(s)	959, 1176, 1269, 1544, 1614, 1754
MluNI	TGGCCA	2 cut(s)	635, 1316
MlyI	GAGTC	1 cut(s)	590
MmeI	TCCRAC	3 cut(s)	408, 435, 2001
Mox20I	TGGCCA	2 cut(s)	635, 1316
Mph1103I	ATGCAT	1 cut(s)	1662
MroI	TCCGGA	1 cut(s)	1969
MscI	TGGCCA	2 cut(s)	635, 1316
MseI	TTAA	7 cut(s)	1089, 1485, 1548, 1617, 1761, 2030, 2055
MslI	CAYNNNNRTG	2 cut(s)	641, 1655
Msp20I	TGGCCA	2 cut(s)	635, 1316
MspA1I	CMGCKG	2 cut(s)	431, 1908
MspI	CCGG	6 cut(s)	43, 399, 450, 872, 1394, 1970
MspR9I	CCNGG	4 cut(s)	311, 1068, 1395, 1517
Mva1269I	GAATGC	1 cut(s)	718
MvaI	CCWGG	3 cut(s)	311, 1068, 1517
MvnI	CGCG	3 cut(s)	114, 185, 389
NciI	CCSGG	1 cut(s)	1395
NdeII	GATC	9 cut(s)	223, 328, 504, 526, 865, 1018, 1144, 1455, 2009
NlaIV	GGNNCC	2 cut(s)	1558, 2167
NmeAIII	GCCGAG	1 cut(s)	640
NsbI	TGCGCA	1 cut(s)	2121
NsiI	ATGCAT	1 cut(s)	1662
NspI	RCATGY	3 cut(s)	1660, 1664, 2126
NspV	TTCGAA	1 cut(s)	24
PaeI	GCATGC	1 cut(s)	2126
PagI	TCATGA	1 cut(s)	708
PaqCI	CACCTGC	2 cut(s)	1682, 2002
PceI	AGGCCT	1 cut(s)	2137
PcsI	WCGNNNNNNNCGW	6 cut(s)	132, 141, 165, 171, 228, 1930
PctI	GAATGC	1 cut(s)	718
PfeI	GAWTC	7 cut(s)	21, 250, 407, 467, 1830, 1847, 1973
PflFI	GACNNNGTC	1 cut(s)	170
PflMI	CCANNNNNTGG	1 cut(s)	862
PleI	GAGTC	1 cut(s)	589
PpsI	GAGTC	1 cut(s)	589
PpuMI	RGGWCCY	1 cut(s)	893
Psp124BI	GAGCTC	1 cut(s)	1803
Psp1406I	AACGTT	2 cut(s)	1217, 1256
Psp5II	RGGWCCY	1 cut(s)	893
Psp6I	CCWGG	3 cut(s)	309, 1066, 1515
PspFI	CCCAGC	1 cut(s)	72
PspGI	CCWGG	3 cut(s)	309, 1066, 1515
PspN4I	GGNNCC	2 cut(s)	1558, 2167
PspPI	GGNCC	3 cut(s)	893, 1513, 1807
PspPPI	RGGWCCY	1 cut(s)	893
PstI	CTGCAG	1 cut(s)	1495
PsyI	GACNNNGTC	1 cut(s)	170
RsaI	GTAC	4 cut(s)	308, 620, 660, 1347
RsaNI	GTAC	4 cut(s)	307, 619, 659, 1346
RseI	CAYNNNNRTG	2 cut(s)	641, 1655
SacI	GAGCTC	1 cut(s)	1803
SalI	GTCGAC	1 cut(s)	173
SaqAI	TTAA	7 cut(s)	1089, 1485, 1548, 1617, 1761, 2030, 2055
Sau3AI	GATC	9 cut(s)	223, 328, 504, 526, 865, 1018, 1144, 1455, 2009
Sau96I	GGNCC	3 cut(s)	893, 1513, 1807
SchI	GAGTC	1 cut(s)	590
ScrFI	CCNGG	4 cut(s)	311, 1068, 1395, 1517
SduI	GDGCHC	2 cut(s)	916, 1803
SfaNI	GCATC	6 cut(s)	223, 436, 987, 1098, 1348, 2234
SfcI	CTRYAG	4 cut(s)	801, 897, 1185, 1491
SfuI	TTCGAA	1 cut(s)	24
SgrDI	CGTCGACG	1 cut(s)	173
SinI	GGWCC	3 cut(s)	893, 1513, 1807
SmiI	ATTTAAAT	1 cut(s)	1549
SmiMI	CAYNNNNRTG	2 cut(s)	641, 1655
SmlI	CTYRAG	2 cut(s)	346, 1332
SmoI	CTYRAG	2 cut(s)	346, 1332
SphI	GCATGC	1 cut(s)	2126
Sse9I	AATT	6 cut(s)	959, 1176, 1269, 1544, 1614, 1754
SseBI	AGGCCT	1 cut(s)	2137
SspMI	CTAG	6 cut(s)	921, 947, 1127, 1263, 1524, 2236
SstI	GAGCTC	1 cut(s)	1803
StuI	AGGCCT	1 cut(s)	2137
StyD4I	CCNGG	4 cut(s)	309, 1066, 1393, 1515
StyI	CCWWGG	3 cut(s)	435, 662, 1159
SwaI	ATTTAAAT	1 cut(s)	1549
TaaI	ACNGT	9 cut(s)	689, 756, 1211, 1297, 1743, 1919, 1982, 2111, 2209
TaiI	ACGT	6 cut(s)	99, 174, 680, 1220, 1259, 1846
TasI	AATT	6 cut(s)	959, 1176, 1269, 1544, 1614, 1754
TatI	WGTACW	1 cut(s)	1345
TauI	GCSGC	5 cut(s)	182, 185, 280, 431, 2018
TfiI	GAWTC	7 cut(s)	21, 250, 407, 467, 1830, 1847, 1973
Tru1I	TTAA	7 cut(s)	1089, 1485, 1548, 1617, 1761, 2030, 2055
Tru9I	TTAA	7 cut(s)	1089, 1485, 1548, 1617, 1761, 2030, 2055
TscAI	CASTG	6 cut(s)	675, 761, 1107, 1629, 1800, 1924
TseI	GCWGC	9 cut(s)	431, 534, 830, 934, 1490, 1493, 1589, 1905, 2159
TspGWI	ACGGA	1 cut(s)	971
TspRI	CASTG	6 cut(s)	675, 761, 1107, 1629, 1800, 1924
Tth111I	GACNNNGTC	1 cut(s)	170
Van91I	CCANNNNNTGG	1 cut(s)	862
VneI	GTGCAC	1 cut(s)	912
VpaK11BI	GGWCC	3 cut(s)	893, 1513, 1807
XapI	RAATTY	3 cut(s)	959, 1269, 1544
XceI	RCATGY	3 cut(s)	1660, 1664, 2126
XcmI	CCANNNNNNNNNTGG	1 cut(s)	373
XmiI	GTMKAC	2 cut(s)	174, 423
XspI	CTAG	6 cut(s)	921, 947, 1127, 1263, 1524, 2236
ZraI	GACGTC	1 cut(s)	172
Zsp2I	ATGCAT	1 cut(s)	1662

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.