Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0003007

Physical Location & Seq

Genomic Coordinates Forward (+)

33572748 .. 33577794

Gene Structure 5047 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1274100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2337 bp

ATGTCGAAGAGAAAATTCGGATTCGAAGGCTTTGGCATAAACAAGCAATCCTCCTTCGACTTCGAGCGGCCCCAGCAAGCCCCTCAGCGGCTCTACGTCCCTCCGTCGTCGCGAGGCGGAAACAGCCACGACAACTACGAAGACGCCGACCTCGACAACATCGATTACGACGACAACGATGGTTCCAAGGACCCCGGGAGTGACGACAACAACCACGGAAACGGTGGCGGCGGTGATGAGGAAGTCGATCCTCTGGATGCTTTCATGGAGGGGATTCACGAGGAGGTGAGGTCAGCTCCGCCGCCGAAGCCGAAAGCGAAGGCCGAGAAGTACAAGGACGATGAGGAGGAGGATCATATGGAGAGTTTTCTGAGTGCTAAGAAGGACGTGGTACTCACGCTGGCATCGGATGCTTTACACGCCGGATATGACTCCGATGAGGAGGTATATGCGGCAGCCAAGGCCGTCGATGCCGGGTTGTTGGAGTACGATTCAGATGATAATCCCATTGTTCTGGACAAGAGGAAGATCGAGCCGATTCCAGCTCTCGATCATAGTTCAATCGACTACGAGCCGTTTAATAAGGATTTTTACGAGGAGAAAGAGTCGATTTCAGGGATGAGTGAGGAGGATGTTTTTGAGTACAAGAAGAGCTTGGCCATCCGTACGTCGGGTTTTGATGTACCGAGGCCAGTTAAGACATTTGAAGATTGTGGATTTTCTTCACAACTCATGGCTGCCATAAAAAAACAAGACTACCAAAAGCCGACGCCAATACAATGCGCAGCTCTACCCATAGTTCTTTCCGGGAGAGATATAATTGGTATTGCGAAAACTGGTTCTGGAAAGACTGCCGCTTTTGTCCTTCCGATGATTGTCCACATTATGGATCAGCCAGAACTTCAAAAAGAAGAAGGTCCCATTGGAGTGATATGTGCTCCTACTAGAGAGCTAGCGCACCAAATATACTTAGAGTCCAAAAAATTTGCGAAATCACATGGGATACGTGTCTGTGCTGTATATGGTGGAATGTCTAAGCTTGATCAGTTCAAAGAACTTAAGGCAGGATGTGAAATAGTTGTTGCTACTCCTGGGAGATTGATAGACATGCTTAAAATGAAGGCACTGACAATGCAAAGGGCAACTTACCTAATACTTGATGAGGCTGATCGGATGTTTGACCTTGGGTTTGAGCCTCAAATTAGATCTATTGTTGGTCAGATTCGGCCAGACCGACAGACATTGCTCTTTTCTGCGACAATGCCCCGTAAAGTTGAAAAATTGGCTAGGGAGATTCTCTCAGATCCTATAAGAGTTACAGTTGGTGAGGTGGGAATGGCCAATGAGGATATCACTCAGGTTGTTCATGTAATTCCTTCTGATGATGAGAAGCTGCCCTGGCTTCTTGAGAAGTTACCTGGAATGATTGATGAGAGTGATGTTTTAGTATTTGCTTCAAAAAAAGCTGCAGTGGATGAGATCGAGTCACAGCTTGCTCAGAAAGGTTTTAAAGTCACAGCTCTGCATGGTGATAAGGACCAGGCATCTCGGATGGATATTTTGCAAAAATTTAAATCTGGCATTTACCATGTTCTTGTTGCAACTGATGTTGCTGCTCGTGGTCTTGATATCAAGTCAATTAAGTCAGTTGTGAACTTTGATATTGCAAAAGACATGGACATGCACGTCCATCGAATTGGTAGAACAGGTCGGGCTGGTGATAAGGATGGCACTGCATACACTCTTATTACACAGAAAGAGGCACGTTTTGCTGGTGAGTTGGTTAATAGCTTGGTTGCTGCTGGTCAGATTGTTTCCACGGAGCTCATGGACCTTGCGATGAAGGATGGTAGATTCAGGTCAAAACGTGATTCAAGAAAAGGAGGTGGAAAGAAAGGTAGAGGGAGGGGCAGCGGTGGTCGAGGTGTGCGTGGGGTGGATTTTGGTCTGGGTATTGGATATAATACGGAGTCCAATAATTCTTCATCGAATACTGTTGCTAGTCGATCTGCTGCAGTAACTCCTCAGAGGACAGGAATGATGTCTCAGTTCAAGACTAAATTTGTTGCCGCTTCATCCAACTCTCCTAGTCATGGTTCAGGTAACAGCTCTAGTGTGCCCAGCAGACCGGCATTACGAGGATTTGTATCCGGTGGTTCAATCGGTGGGGATGTATATAGAACTCAGACAACCAGTACAATTACTCCTGCTCCTCCTACATCAGTGATAAATAAAACTGGTCCTCAGAACACTGGAGAAAATTCAAGTCAGAAACCCTCTGAAAGTTCTAGAGAGAAACCTAGAGAAAGGCGGAGGCGCTCCGGTTGGGACTGTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0006139 GO:0006725 GO:0006807 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0009987 GO:0010501 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0031974 GO:0031981 GO:0034641 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0070013 GO:0070035 GO:0071704 GO:0090304 GO:0140098 GO:1901360

KEGG Pathways

Metabolic & Signaling

ko03040 map03040

Pfam Domains

Protein Families

DEAD Helicase_C

Protein Analysis

779

Amino Acids

85.01

Weight (kDa)

5.82

Isoelectric Point (pI)

43.44

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DEAD	PF00270	257 - 427	3.3e-51	DEAD/DEAH box helicase
Helicase_C	PF00271	476 - 573	1.5e-26	Helicase conserved C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0003007)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G47330
fragaria_vesca	FvH4_1g27100 FvH4_1g27100 FvH4_1g27100
malus_domestica	MD00G1118300.v1.1 MD00G1118400.v1.1 MD09G1283400.v1.1 MD17G1274100.v1.1 MD17G1274800.v1.1 MD17G1275300.v1.1 MD17G1276500.v1.1
prunus_persica	Prupe.3G010900_v2.0.a1 Prupe.3G010900_v2.0.a1 Prupe.3G010900_v2.0.a1 Prupe.3G010900_v2.0.a1 Prupe.3G010900_v2.0.a1
pyrus_communis	pycom09g19130 pycom09g19140 pycom17g27210 pycom17g27330
rosa_chinensis	RchiOBHm_Chr3g0487421
rosa_laevigata	RLG00000023004
rosa_multiflora	Rmu_sc0008232.1_g000009 Rmu_sc0022533.1_g000001 Rmu_sc0031316.1_g000001
rosa_roxburghii	Rroxscaffold_6G00394790
rosa_rugosa	Rorug03G0234000
rosa_samantha	Rh2AG395100 Rh2CG381700 Rh3AG282000 Rh3BG317600 Rh3CG315700 Rh3DG313600 Rh4CG235900 Rh7AG337700 Rh7AG476200 Rh7CG355800
rosa_wichuraiana	Rw3G024970

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1685
Acc16I	TGCGCA	1 cut(s)	784
AccB7I	CCANNNNNTGG	1 cut(s)	886
AccBSI	CCGCTC	1 cut(s)	67
AccII	CGCG	1 cut(s)	112
AclWI	GGATC	4 cut(s)	242, 360, 897, 1298
AcoI	YGGCCR	3 cut(s)	657, 1226, 1338
AcsI	RAATTY	5 cut(s)	14, 983, 1568, 2060, 2260
AcyI	GRCGYC	2 cut(s)	144, 770
AfaI	GTAC	7 cut(s)	332, 393, 488, 644, 667, 684, 2197
AfiI	CCNNNNNNNGG	4 cut(s)	87, 670, 886, 2093
AflII	CTTAAG	1 cut(s)	1058
AflIII	ACRYGT	1 cut(s)	1006
AjiI	CACGTC	2 cut(s)	388, 1687
AjnI	CCWGG	4 cut(s)	1090, 1397, 1417, 1539
AjuI	GAANNNNNNNTTGG	2 cut(s)	906, 938
Alw21I	GWGCWC	2 cut(s)	940, 1827
Alw26I	GTCTC	1 cut(s)	2049
AlwI	GGATC	4 cut(s)	242, 360, 897, 1298
Ama87I	CYCGRG	1 cut(s)	194
AoxI	GGCC	7 cut(s)	68, 321, 462, 657, 689, 1226, 1338
ApeKI	GCWGC	9 cut(s)	455, 737, 785, 1393, 1466, 1613, 1799, 1911, 2012
ApoI	RAATTY	5 cut(s)	14, 983, 1568, 2060, 2260
ArsI	GACNNNNNNTTYG	2 cut(s)	1172, 1204
AspLEI	GCGC	3 cut(s)	785, 958, 2319
AspS9I	GGNCC	6 cut(s)	69, 190, 917, 1537, 1831, 2240
AsuC2I	CCSGG	4 cut(s)	195, 196, 475, 808
AsuHPI	GGTGA	6 cut(s)	245, 298, 1337, 1541, 1730, 1787
AsuII	TTCGAA	1 cut(s)	24
AsuNHI	GCTAGC	1 cut(s)	952
AvaI	CYCGRG	1 cut(s)	194
AvaII	GGWCC	5 cut(s)	190, 917, 1537, 1831, 2240
BaeGI	GKGCMC	1 cut(s)	2121
BalI	TGGCCA	2 cut(s)	659, 1340
BanII	GRGCYC	1 cut(s)	1827
BauI	CACGAG	2 cut(s)	278, 1617
BbsI	GAAGAC	1 cut(s)	147
Bbv12I	GWGCWC	2 cut(s)	940, 1827
BbvCI	CCTCAGC	1 cut(s)	84
BbvI	GCAGC	9 cut(s)	467, 724, 797, 1380, 1453, 1600, 1786, 1923, 1999
BccI	CCATC	6 cut(s)	173, 668, 1546, 1698, 1721, 1841
BceAI	ACGGC	2 cut(s)	449, 559
BcgI	CGANNNNNNTGC	2 cut(s)	1673, 1707
BciT130I	CCWGG	4 cut(s)	1092, 1399, 1419, 1541
BciVI	GTATCC	2 cut(s)	996, 2158
BclI	TGATCA	1 cut(s)	1042
BcnI	CCSGG	4 cut(s)	195, 196, 475, 808
BcoDI	GTCTC	1 cut(s)	2049
BfaI	CTAG	8 cut(s)	945, 953, 1287, 2001, 2088, 2112, 2289, 2301
BfmI	CTRYAG	2 cut(s)	1467, 2013
BfoI	RGCGCY	1 cut(s)	2320
BfrI	CTTAAG	1 cut(s)	1058
BfuI	GTATCC	2 cut(s)	996, 2158
BglII	AGATCT	1 cut(s)	1205
Bme1390I	CCNGG	8 cut(s)	195, 196, 475, 808, 1092, 1399, 1419, 1541
Bme18I	GGWCC	5 cut(s)	190, 917, 1537, 1831, 2240
BmeT110I	CYCGRG	1 cut(s)	194
BmgBI	CACGTC	2 cut(s)	388, 1687
BmgT120I	GGNCC	6 cut(s)	69, 190, 917, 1537, 1831, 2240
BmiI	GGNNCC	4 cut(s)	71, 184, 192, 919
BmrFI	CCNGG	8 cut(s)	195, 196, 475, 808, 1092, 1399, 1419, 1541
BmsI	GCATC	5 cut(s)	247, 400, 413, 460, 1553
BmtI	GCTAGC	1 cut(s)	956
BpiI	GAAGAC	1 cut(s)	147
BplI	GAGNNNNNCTC	4 cut(s)	280, 312, 1283, 1315
BpmI	CTGGAG	1 cut(s)	2274
Bpu10I	CCTNAGC	1 cut(s)	84
Bpu14I	TTCGAA	1 cut(s)	24
BpuEI	CTTGAG	1 cut(s)	1427
BpuMI	CCSGG	4 cut(s)	195, 196, 475, 808
Bsa29I	ATCGAT	1 cut(s)	162
BsaAI	YACGTR	1 cut(s)	1007
BsaBI	GATNNNNATC	2 cut(s)	501, 2146
BsaHI	GRCGYC	2 cut(s)	144, 770
BsaWI	WCCGGW	2 cut(s)	2150, 2321
BsaXI	ACNNNNNCTCC	8 cut(s)	275, 305, 590, 620, 2188, 2194, 2218, 2224
Bsc4I	CCNNNNNNNGG	4 cut(s)	87, 670, 886, 2093
Bse118I	RCCGGY	1 cut(s)	2128
Bse1I	ACTGG	5 cut(s)	692, 841, 2193, 2242, 2257
Bse3DI	GCAATG	1 cut(s)	1241
Bse8I	GATNNNNATC	2 cut(s)	501, 2146
BseBI	CCWGG	4 cut(s)	1092, 1399, 1419, 1541
BseCI	ATCGAT	1 cut(s)	162
BseJI	GATNNNNATC	2 cut(s)	501, 2146
BseLI	CCNNNNNNNGG	4 cut(s)	87, 670, 886, 2093
BseMI	GCAATG	1 cut(s)	1241
BseMII	CTCAG	9 cut(s)	98, 362, 1314, 1370, 1511, 2039, 2060, 2198, 2258
BseNI	ACTGG	5 cut(s)	692, 841, 2193, 2242, 2257
BseRI	GAGGAG	8 cut(s)	296, 359, 362, 455, 611, 641, 2013, 2202
BseSI	GKGCMC	1 cut(s)	2121
BseXI	GCAGC	9 cut(s)	467, 724, 797, 1380, 1453, 1600, 1786, 1923, 1999
BseYI	CCCAGC	2 cut(s)	72, 2120
Bsh1236I	CGCG	1 cut(s)	112
BshFI	GGCC	7 cut(s)	70, 323, 464, 659, 691, 1228, 1340
BshVI	ATCGAT	1 cut(s)	162
BsiHKAI	GWGCWC	2 cut(s)	940, 1827
BsiHKCI	CYCGRG	1 cut(s)	194
BsiSI	CCGG	7 cut(s)	195, 423, 474, 807, 2129, 2151, 2322
BsiWI	CGTACG	1 cut(s)	665
BslFI	GGGAC	2 cut(s)	83, 903
BslI	CCNNNNNNNGG	4 cut(s)	87, 670, 886, 2093
BsmAI	GTCTC	1 cut(s)	2049
BsmFI	GGGAC	2 cut(s)	83, 903
BsnI	GGCC	7 cut(s)	70, 323, 464, 659, 691, 1228, 1340
BsoBI	CYCGRG	1 cut(s)	194
Bsp119I	TTCGAA	1 cut(s)	24
Bsp1286I	GDGCHC	3 cut(s)	940, 1827, 2121
Bsp68I	TCGCGA	1 cut(s)	112
BspANI	GGCC	7 cut(s)	70, 323, 464, 659, 691, 1228, 1340
BspCNI	CTCAG	9 cut(s)	97, 363, 1313, 1369, 1510, 2038, 2059, 2197, 2257
BspDI	ATCGAT	1 cut(s)	162
BspFNI	CGCG	1 cut(s)	112
BspLI	GGNNCC	4 cut(s)	71, 184, 192, 919
BspMAI	CTGCAG	2 cut(s)	1471, 2017
BspOI	GCTAGC	1 cut(s)	956
BspPI	GGATC	4 cut(s)	242, 360, 897, 1298
BspQI	GCTCTTC	1 cut(s)	644
BspT104I	TTCGAA	1 cut(s)	24
BspTI	CTTAAG	1 cut(s)	1058
BsrBI	CCGCTC	1 cut(s)	67
BsrDI	GCAATG	1 cut(s)	1241
BsrFI	RCCGGY	1 cut(s)	2128
BsrI	ACTGG	5 cut(s)	692, 841, 2193, 2242, 2257
BssAI	RCCGGY	1 cut(s)	2128
BssNI	GRCGYC	2 cut(s)	144, 770
BssSI	CACGAG	2 cut(s)	278, 1617
BssT1I	CCWWGG	3 cut(s)	186, 459, 1183
Bst2BI	CACGAG	2 cut(s)	278, 1617
Bst2UI	CCWGG	4 cut(s)	1092, 1399, 1419, 1541
Bst4CI	ACNGT	4 cut(s)	224, 1321, 1996, 2333
Bst6I	CTCTTC	2 cut(s)	2, 644
BstACI	GRCGYC	2 cut(s)	144, 770
BstAFI	CTTAAG	1 cut(s)	1058
BstAPI	GCANNNNNTGC	2 cut(s)	410, 1769
BstBAI	YACGTR	1 cut(s)	1007
BstBI	TTCGAA	1 cut(s)	24
BstC8I	GCNNGC	4 cut(s)	78, 402, 954, 1494
BstDSI	CCRYGG	2 cut(s)	214, 1818
BstFNI	CGCG	1 cut(s)	112
BstH2I	RGCGCY	1 cut(s)	2320
BstHHI	GCGC	3 cut(s)	785, 958, 2319
BstMAI	GTCTC	1 cut(s)	2049
BstMWI	GCNNNNNNNGC	9 cut(s)	73, 123, 307, 410, 419, 461, 470, 1399, 1769
BstNI	CCWGG	4 cut(s)	1092, 1399, 1419, 1541
BstNSI	RCATGY	2 cut(s)	1111, 1684
BstSCI	CCNGG	8 cut(s)	193, 194, 473, 806, 1090, 1397, 1417, 1539
BstSFI	CTRYAG	2 cut(s)	1467, 2013
BstSLI	GKGCMC	1 cut(s)	2121
BstUI	CGCG	1 cut(s)	112
BstV1I	GCAGC	9 cut(s)	467, 724, 797, 1380, 1453, 1600, 1786, 1923, 1999
BstV2I	GAAGAC	1 cut(s)	147
BstX2I	RGATCY	2 cut(s)	1205, 1303
BstXI	CCANNNNNNTGG	2 cut(s)	514, 1697
BstYI	RGATCY	2 cut(s)	1205, 1303
Bsu15I	ATCGAT	1 cut(s)	162
BsuI	GTATCC	2 cut(s)	996, 2158
BsuRI	GGCC	7 cut(s)	70, 323, 464, 659, 691, 1228, 1340
BsuTUI	ATCGAT	1 cut(s)	162
BtgI	CCRYGG	2 cut(s)	214, 1818
BtgZI	GCGATG	1 cut(s)	1853
BtrI	CACGTC	2 cut(s)	388, 1687
BtsI	GCAGTG	2 cut(s)	1476, 1731
BtsIMutI	CAGTG	5 cut(s)	1124, 1476, 1731, 2229, 2250
BtuMI	TCGCGA	1 cut(s)	112
Cac8I	GCNNGC	4 cut(s)	78, 402, 954, 1494
CfoI	GCGC	3 cut(s)	785, 958, 2319
Cfr10I	RCCGGY	1 cut(s)	2128
Cfr13I	GGNCC	6 cut(s)	69, 190, 917, 1537, 1831, 2240
Cfr9I	CCCGGG	1 cut(s)	194
ClaI	ATCGAT	1 cut(s)	162
CseI	GACGC	2 cut(s)	152, 778
Csp6I	GTAC	7 cut(s)	331, 392, 487, 643, 666, 683, 2196
CviQI	GTAC	7 cut(s)	331, 392, 487, 643, 666, 683, 2196
DraI	TTTAAA	2 cut(s)	1510, 1573
DrdI	GACNNNNNNGTC	1 cut(s)	1685
DseDI	GACNNNNNNGTC	1 cut(s)	1685
EaeI	YGGCCR	3 cut(s)	657, 1226, 1338
Eam1104I	CTCTTC	2 cut(s)	2, 644
EarI	CTCTTC	2 cut(s)	2, 644
EciI	GGCGGA	3 cut(s)	132, 288, 2326
Ecl136II	GAGCTC	1 cut(s)	1825
Eco130I	CCWWGG	3 cut(s)	186, 459, 1183
Eco24I	GRGCYC	1 cut(s)	1827
Eco32I	GATATC	2 cut(s)	1351, 1630
Eco47I	GGWCC	5 cut(s)	190, 917, 1537, 1831, 2240
Eco53kI	GAGCTC	1 cut(s)	1825
Eco88I	CYCGRG	1 cut(s)	194
EcoICRI	GAGCTC	1 cut(s)	1825
EcoO109I	RGGNCCY	2 cut(s)	190, 917
EcoRII	CCWGG	4 cut(s)	1090, 1397, 1417, 1539
EcoRV	GATATC	2 cut(s)	1351, 1630
EcoT14I	CCWWGG	3 cut(s)	186, 459, 1183
EcoT38I	GRGCYC	1 cut(s)	1827
ErhI	CCWWGG	3 cut(s)	186, 459, 1183
FalI	AAGNNNNNCTT	4 cut(s)	638, 670, 1129, 1161
FaqI	GGGAC	2 cut(s)	83, 903
FauNDI	CATATG	1 cut(s)	357
FbaI	TGATCA	1 cut(s)	1042
FriOI	GRGCYC	1 cut(s)	1827
FspBI	CTAG	8 cut(s)	945, 953, 1287, 2001, 2088, 2112, 2289, 2301
FspI	TGCGCA	1 cut(s)	784
GlaI	GCGC	3 cut(s)	784, 957, 2318
GsaI	CCCAGC	2 cut(s)	76, 2124
GsuI	CTGGAG	1 cut(s)	2274
HaeII	RGCGCY	1 cut(s)	2320
HaeIII	GGCC	7 cut(s)	70, 323, 464, 659, 691, 1228, 1340
HapII	CCGG	7 cut(s)	195, 423, 474, 807, 2129, 2151, 2322
HgaI	GACGC	2 cut(s)	152, 778
HhaI	GCGC	3 cut(s)	785, 958, 2319
Hin1I	GRCGYC	2 cut(s)	144, 770
Hin6I	GCGC	3 cut(s)	783, 956, 2317
HinP1I	GCGC	3 cut(s)	783, 956, 2317
HindIII	AAGCTT	1 cut(s)	1037
HpaII	CCGG	7 cut(s)	195, 423, 474, 807, 2129, 2151, 2322
HphI	GGTGA	6 cut(s)	245, 298, 1337, 1541, 1730, 1787
Hpy166II	GTNNAC	2 cut(s)	880, 1654
Hpy8I	GTNNAC	2 cut(s)	880, 1654
Hpy99I	CGWCG	6 cut(s)	109, 112, 173, 470, 673, 772
HpyAV	CCTTC	9 cut(s)	20, 64, 313, 376, 875, 908, 1114, 1386, 1837
HpyCH4III	ACNGT	4 cut(s)	224, 1321, 1996, 2333
HpyCH4IV	ACGT	7 cut(s)	96, 387, 668, 1006, 1686, 1765, 1867
HpyCH4V	TGCA	9 cut(s)	1135, 1469, 1525, 1564, 1601, 1667, 1684, 1736, 2015
HpyF10VI	GCNNNNNNNGC	9 cut(s)	73, 123, 307, 410, 419, 461, 470, 1399, 1769
HpySE526I	ACGT	7 cut(s)	96, 387, 668, 1006, 1686, 1765, 1867
Hsp92I	GRCGYC	2 cut(s)	144, 770
HspAI	GCGC	3 cut(s)	783, 956, 2317
Ksp22I	TGATCA	1 cut(s)	1042
LguI	GCTCTTC	1 cut(s)	644
LmnI	GCTCC	5 cut(s)	301, 943, 1822, 2215, 2324
Lsp1109I	GCAGC	9 cut(s)	467, 724, 797, 1380, 1453, 1600, 1786, 1923, 1999
LweI	GCATC	5 cut(s)	247, 400, 413, 460, 1553
MaeI	CTAG	8 cut(s)	945, 953, 1287, 2001, 2088, 2112, 2289, 2301
MaeII	ACGT	7 cut(s)	96, 387, 668, 1006, 1686, 1765, 1867
MaeIII	GTNAC	7 cut(s)	200, 1315, 1413, 1485, 1513, 2017, 2102
MbiI	CCGCTC	1 cut(s)	67
MboII	GAAGA	8 cut(s)	19, 152, 538, 661, 714, 719, 923, 1974
MflI	RGATCY	2 cut(s)	1205, 1303
MhlI	GDGCHC	3 cut(s)	940, 1827, 2121
MlsI	TGGCCA	2 cut(s)	659, 1340
MluNI	TGGCCA	2 cut(s)	659, 1340
MlyI	GAGTC	5 cut(s)	425, 614, 983, 1493, 1979
MmeI	TCCRAC	2 cut(s)	462, 2103
Mox20I	TGGCCA	2 cut(s)	659, 1340
MscI	TGGCCA	2 cut(s)	659, 1340
MseI	TTAA	9 cut(s)	579, 696, 1059, 1113, 1509, 1572, 1641, 1785, 2335
MslI	CAYNNNNRTG	2 cut(s)	926, 1679
Msp20I	TGGCCA	2 cut(s)	659, 1340
MspA1I	CMGCKG	2 cut(s)	88, 1914
MspCI	CTTAAG	1 cut(s)	1058
MspI	CCGG	7 cut(s)	195, 423, 474, 807, 2129, 2151, 2322
MspR9I	CCNGG	8 cut(s)	195, 196, 475, 808, 1092, 1399, 1419, 1541
MvaI	CCWGG	4 cut(s)	1092, 1399, 1419, 1541
MvnI	CGCG	1 cut(s)	112
MwoI	GCNNNNNNNGC	9 cut(s)	73, 123, 307, 410, 419, 461, 470, 1399, 1769
NciI	CCSGG	4 cut(s)	195, 196, 475, 808
NdeI	CATATG	1 cut(s)	357
NheI	GCTAGC	1 cut(s)	952
NlaIV	GGNNCC	4 cut(s)	71, 184, 192, 919
NmeAIII	GCCGAG	1 cut(s)	349
NmuCI	GTSAC	3 cut(s)	200, 1485, 1513
NruI	TCGCGA	1 cut(s)	112
NsbI	TGCGCA	1 cut(s)	784
NspI	RCATGY	2 cut(s)	1111, 1684
NspV	TTCGAA	1 cut(s)	24
PciSI	GCTCTTC	1 cut(s)	644
PcsI	WCGNNNNNNNCGW	8 cut(s)	135, 144, 150, 159, 168, 174, 1231, 1927
PfeI	GAWTC	8 cut(s)	21, 274, 491, 538, 1222, 1294, 1854, 1871
Pfl23II	CGTACG	1 cut(s)	665
PflMI	CCANNNNNTGG	1 cut(s)	886
PfoI	TCCNGGA	1 cut(s)	806
PleI	GAGTC	5 cut(s)	425, 613, 982, 1492, 1978
PpsI	GAGTC	5 cut(s)	425, 613, 982, 1492, 1978
Ppu21I	YACGTR	1 cut(s)	1007
PpuMI	RGGWCCY	2 cut(s)	190, 917
Psp124BI	GAGCTC	1 cut(s)	1827
Psp5II	RGGWCCY	2 cut(s)	190, 917
Psp6I	CCWGG	4 cut(s)	1090, 1397, 1417, 1539
PspFI	CCCAGC	2 cut(s)	72, 2120
PspGI	CCWGG	4 cut(s)	1090, 1397, 1417, 1539
PspLI	CGTACG	1 cut(s)	665
PspN4I	GGNNCC	4 cut(s)	71, 184, 192, 919
PspPI	GGNCC	6 cut(s)	69, 190, 917, 1537, 1831, 2240
PspPPI	RGGWCCY	2 cut(s)	190, 917
PstI	CTGCAG	2 cut(s)	1471, 2017
PsuI	RGATCY	2 cut(s)	1205, 1303
RruI	TCGCGA	1 cut(s)	112
RsaI	GTAC	7 cut(s)	332, 393, 488, 644, 667, 684, 2197
RsaNI	GTAC	7 cut(s)	331, 392, 487, 643, 666, 683, 2196
RseI	CAYNNNNRTG	2 cut(s)	926, 1679
SacI	GAGCTC	1 cut(s)	1827
SapI	GCTCTTC	1 cut(s)	644
SaqAI	TTAA	9 cut(s)	579, 696, 1059, 1113, 1509, 1572, 1641, 1785, 2335
Sau96I	GGNCC	6 cut(s)	69, 190, 917, 1537, 1831, 2240
SchI	GAGTC	5 cut(s)	425, 614, 983, 1493, 1979
ScrFI	CCNGG	8 cut(s)	195, 196, 475, 808, 1092, 1399, 1419, 1541
SduI	GDGCHC	3 cut(s)	940, 1827, 2121
SfaNI	GCATC	5 cut(s)	247, 400, 413, 460, 1553
SfcI	CTRYAG	2 cut(s)	1467, 2013
SfuI	TTCGAA	1 cut(s)	24
SinI	GGWCC	5 cut(s)	190, 917, 1537, 1831, 2240
SmaI	CCCGGG	1 cut(s)	196
SmiI	ATTTAAAT	1 cut(s)	1573
SmiMI	CAYNNNNRTG	2 cut(s)	926, 1679
SmlI	CTYRAG	2 cut(s)	1058, 1406
SmoI	CTYRAG	2 cut(s)	1058, 1406
SspMI	CTAG	8 cut(s)	945, 953, 1287, 2001, 2088, 2112, 2289, 2301
SstI	GAGCTC	1 cut(s)	1827
StyD4I	CCNGG	8 cut(s)	193, 194, 473, 806, 1090, 1397, 1417, 1539
StyI	CCWWGG	3 cut(s)	186, 459, 1183
SwaI	ATTTAAAT	1 cut(s)	1573
TaaI	ACNGT	4 cut(s)	224, 1321, 1996, 2333
TaiI	ACGT	7 cut(s)	99, 390, 671, 1009, 1689, 1768, 1870
TaqII	GACCGA	1 cut(s)	1248
TatI	WGTACW	3 cut(s)	330, 642, 2195
TauI	GCSGC	7 cut(s)	70, 91, 231, 304, 455, 857, 2072
TfiI	GAWTC	8 cut(s)	21, 274, 491, 538, 1222, 1294, 1854, 1871
Tru1I	TTAA	9 cut(s)	579, 696, 1059, 1113, 1509, 1572, 1641, 1785, 2335
Tru9I	TTAA	9 cut(s)	579, 696, 1059, 1113, 1509, 1572, 1641, 1785, 2335
TscAI	CASTG	5 cut(s)	1131, 1476, 1738, 2229, 2257
TseFI	GTSAC	3 cut(s)	200, 1485, 1513
TseI	GCWGC	9 cut(s)	455, 737, 785, 1393, 1466, 1613, 1799, 1911, 2012
Tsp45I	GTSAC	3 cut(s)	200, 1485, 1513
TspDTI	ATGAA	6 cut(s)	253, 1133, 1355, 1856, 1974, 2064
TspGWI	ACGGA	5 cut(s)	93, 231, 653, 1835, 1982
TspMI	CCCGGG	1 cut(s)	194
TspRI	CASTG	5 cut(s)	1131, 1476, 1738, 2229, 2257
Van91I	CCANNNNNTGG	1 cut(s)	886
Vha464I	CTTAAG	1 cut(s)	1058
VpaK11BI	GGWCC	5 cut(s)	190, 917, 1537, 1831, 2240
XapI	RAATTY	5 cut(s)	14, 983, 1568, 2060, 2260
XbaI	TCTAGA	1 cut(s)	2288
XceI	RCATGY	2 cut(s)	1111, 1684
XcmI	CCANNNNNNNNNTGG	2 cut(s)	221, 1825
XmaI	CCCGGG	1 cut(s)	194
XspI	CTAG	8 cut(s)	945, 953, 1287, 2001, 2088, 2112, 2289, 2301

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.