Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0003007

Physical Location & Seq

Genomic Coordinates Forward (+)

33658041 .. 33661518

Gene Structure 3478 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1275300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1833 bp

ATGGATAGGTTTTTTAGGGCTAAAAAGGATGTGGTGCTCACACTGGCATCGGGTGCTTTACACGCCGGATATGACTCCGATGAGGAGGTATATGCGACCGCCAAGGCCGTCGATGCCGGGTTGTTGGAGTACGATTCAGATGATAATCCCATTGTTCTGGACAAGAGGAAGATCGAACCGATTCCAGCTCTCGATCATAGTTCAATCGACTACGAGCCGTTTAATAAGGATTTTTACGAGGAGAAATACTTGGCCATCCGTACGTCGGGTTTTGATATACCGAGGCCAGTTAAGACATTTGAAGATTGTGGATTTTCTTCACAACTCATGGCTGCCATAAAAAAACAAGACTACCAAAAGCCGACACCAATACAATGCGCAGCTCTACCCATAGTTCTTTCCGGGAGAGATATAATTGGTATTGCGAAAACTGGTTCTGGAAAGACTGCCACTTTTGTCATTCCAATGATTGTCCACATTATGGATCAGCCAGACCTTCAAAAAGAAAAAGGTCCAATTGGAGCGATATGTGCTTCTACTAGAGAGCTGGTGCACCAAATATACTTAAGACTTGATCAGTTCAAAGAACTTAAGGTAGGATGTGAAATAGTTGTTGCTACTCCTGGGAAATTAATCGACATGCTTAAAATGAAGGCACTGACAATGCTTAGGGCAACTTACCTAGTACTTGATGAGGCTGATCGGATGTTTGACCTTGGGTTTGAGCCTCAAATTAGATCTATTGTTGGTCAGATTCGGCCAGACCAACAGACATTGCTCTTTTCTGCAACAATGCCCCGTAAAGTTGAAAAATTGGCTAGGGAGATTCTCTCAGATCCTATAAGAGTTACAGTGGGTGAGGTGGGAATGGCCAATGAGGATATCACTCAGGTTGTTCATGTAATTCCTTCTGATGATGAGAAGCTGCCCTGGCTTATTGAGAAGTTACCTCGAATGATTGATGAGGGTGATGTTTTAGTATTTCTTGCTCAGAAAGGTTTTAAAGTCACAGCTCTGCATGGTGACAAGGACCAGGCATCTCGGATGGATATTTTGCAAAAATTTAAATCTGGCATTTACCATGTTCTTGTTGCAACTGATGTTGCTGCTCGTGGTCTTGACATCAAGTCAATTAAGTCGATTGTGAACTTTGATATTGCAAAAGACATGGACATGCACGTCCATCGAATTGGTAGAACAGGTCGGGCTGGTGATAAGGATGGCATTGCATACACTCTTATTACACAGAAAGAGGCACGTTTTGCTGGTGAGTTGGTTAATAGCTTGGTTGTTGCTGGTCAGATTGTCTCCACGGAGCTCATGGACCTTGCGATGAAGGATGGTAGATTTAGGTCAAAACGCGATTCAAGAAAAGGAGGTGGAAAGAAAGGTAGAGGGAGGGGCGGCGGTGGTCGAGGTGTGCGTGGGGTGGATTTTGGTCTGGGTATTGGATATAATACGGAGTCCAATAGTTCTTCATCGAATACTATTCCTAGTCGATCTGCTGCAGTAACTCCTCAGAGGACAGGAATGATGTCTCAGTTCAAGACTAAATTTGTTGCCGCTTCATCTAACTCTCCAAGTCAAGGTTTAGCTAACAACTTTAGTGTGCCCAACAGACCGGCATTACGAGGATTTGTATCCGGTGGTTCAATAGGTGGTGATGTATATATAACTCAGGCAACCAGTACAATTACTCCTACTCCTGCTCCAACATCAATGGTAAATAAAACTGCTACTCCGAACACTGGAGAAAATTCAAGTCAGAAACCCTCCGAAAGTTCTAGAGATAAACCCAGAGAAAGGCGGAGGCGCTCCGGTTGGGACTGTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0006139 GO:0006725 GO:0006807 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0009987 GO:0010501 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0031974 GO:0031981 GO:0034641 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0070013 GO:0070035 GO:0071704 GO:0090304 GO:0140098 GO:1901360

KEGG Pathways

Metabolic & Signaling

ko03040 map03040

Pfam Domains

Protein Families

DEAD Helicase_C

Protein Analysis

611

Amino Acids

66.79

Weight (kDa)

9.02

Isoelectric Point (pI)

34.78

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DEAD	PF00270	122 - 271	5.7e-35	DEAD/DEAH box helicase
Helicase_C	PF00271	328 - 404	8.3e-23	Helicase conserved C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003007)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G47330
fragaria_vesca	FvH4_1g27100 FvH4_1g27100 FvH4_1g27100
malus_domestica	MD00G1118300.v1.1 MD00G1118400.v1.1 MD09G1283400.v1.1 MD17G1274100.v1.1 MD17G1274800.v1.1 MD17G1275300.v1.1 MD17G1276500.v1.1
prunus_persica	Prupe.3G010900_v2.0.a1 Prupe.3G010900_v2.0.a1 Prupe.3G010900_v2.0.a1 Prupe.3G010900_v2.0.a1 Prupe.3G010900_v2.0.a1
pyrus_communis	pycom09g19130 pycom09g19140 pycom17g27210 pycom17g27330
rosa_chinensis	RchiOBHm_Chr3g0487421
rosa_laevigata	RLG00000023004
rosa_multiflora	Rmu_sc0008232.1_g000009 Rmu_sc0022533.1_g000001 Rmu_sc0031316.1_g000001
rosa_roxburghii	Rroxscaffold_6G00394790
rosa_rugosa	Rorug03G0234000
rosa_samantha	Rh2AG395100 Rh2CG381700 Rh3AG282000 Rh3BG317600 Rh3CG315700 Rh3DG313600 Rh4CG235900 Rh7AG337700 Rh7AG476200 Rh7CG355800
rosa_wichuraiana	Rw3G024970

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1178
Acc16I	TGCGCA	1 cut(s)	379
AccB7I	CCANNNNNTGG	1 cut(s)	481
AccII	CGCG	1 cut(s)	1362
AciI	CCGC	5 cut(s)	99, 1404, 1407, 1563, 1807
AclWI	GGATC	2 cut(s)	492, 830
AcoI	YGGCCR	3 cut(s)	252, 758, 870
AcsI	RAATTY	3 cut(s)	1061, 1553, 1756
AfaI	GTAC	4 cut(s)	131, 262, 687, 1690
AfiI	CCNNNNNNNGG	5 cut(s)	265, 481, 1586, 1748, 1803
AflII	CTTAAG	2 cut(s)	565, 590
AgsI	TTSAA	9 cut(s)	204, 302, 500, 583, 809, 1368, 1546, 1653, 1761
AhdI	GACNNNNNGTC	1 cut(s)	1126
AjiI	CACGTC	1 cut(s)	1180
AjnI	CCWGG	3 cut(s)	622, 929, 1032
AluBI	AGCT	8 cut(s)	188, 383, 547, 925, 1013, 1284, 1318, 1595
AluI	AGCT	8 cut(s)	188, 383, 547, 925, 1013, 1284, 1318, 1595
Alw21I	GWGCWC	3 cut(s)	39, 555, 1320
Alw26I	GTCTC	2 cut(s)	1312, 1542
Alw44I	GTGCAC	1 cut(s)	551
AlwI	GGATC	2 cut(s)	492, 830
AoxI	GGCC	5 cut(s)	105, 252, 284, 758, 870
ApaLI	GTGCAC	1 cut(s)	551
ApeKI	GCWGC	5 cut(s)	332, 380, 925, 1106, 1505
ApoI	RAATTY	3 cut(s)	1061, 1553, 1756
ArsI	GACNNNNNNTTYG	2 cut(s)	704, 736
AseI	ATTAAT	1 cut(s)	632
Asp700I	GAANNNNTTC	1 cut(s)	180
AspLEI	GCGC	2 cut(s)	380, 1815
AspS9I	GGNCC	3 cut(s)	512, 1030, 1324
AsuC2I	CCSGG	2 cut(s)	118, 403
AsuHPI	GGTGA	6 cut(s)	869, 980, 1034, 1223, 1280, 1673
AvaII	GGWCC	3 cut(s)	512, 1030, 1324
BaeGI	GKGCMC	2 cut(s)	555, 1614
BalI	TGGCCA	2 cut(s)	254, 872
BanII	GRGCYC	1 cut(s)	1320
BauI	CACGAG	1 cut(s)	1110
Bbv12I	GWGCWC	3 cut(s)	39, 555, 1320
BbvI	GCAGC	5 cut(s)	319, 392, 912, 1093, 1492
BccI	CCATC	5 cut(s)	263, 1039, 1191, 1214, 1334
BceAI	ACGGC	2 cut(s)	92, 202
BcgI	CGANNNNNNTGC	2 cut(s)	1166, 1200
BciT130I	CCWGG	3 cut(s)	624, 931, 1034
BciVI	GTATCC	1 cut(s)	1651
BclI	TGATCA	1 cut(s)	574
BcnI	CCSGG	2 cut(s)	118, 403
BcoDI	GTCTC	2 cut(s)	1312, 1542
BfaI	CTAG	5 cut(s)	540, 683, 819, 1494, 1785
BfmI	CTRYAG	1 cut(s)	1506
BfoI	RGCGCY	1 cut(s)	1816
BfrI	CTTAAG	2 cut(s)	565, 590
BfuI	GTATCC	1 cut(s)	1651
BglII	AGATCT	1 cut(s)	737
BisI	GCNGC	7 cut(s)	333, 381, 926, 1107, 1405, 1506, 1563
BlsI	GCNGC	7 cut(s)	334, 382, 927, 1108, 1406, 1507, 1564
BmcAI	AGTACT	1 cut(s)	687
Bme1390I	CCNGG	5 cut(s)	118, 403, 624, 931, 1034
Bme18I	GGWCC	3 cut(s)	512, 1030, 1324
BmeRI	GACNNNNNGTC	1 cut(s)	1126
BmgBI	CACGTC	1 cut(s)	1180
BmgT120I	GGNCC	3 cut(s)	512, 1030, 1324
BmrFI	CCNGG	5 cut(s)	118, 403, 624, 931, 1034
BmsI	GCATC	3 cut(s)	56, 103, 1046
BplI	GAGNNNNNCTC	2 cut(s)	815, 847
BpmI	CTGGAG	1 cut(s)	1770
Bpu10I	CCTNAGC	1 cut(s)	668
BpuMI	CCSGG	2 cut(s)	118, 403
BsaBI	GATNNNNATC	2 cut(s)	144, 1639
BsaJI	CCNNGG	6 cut(s)	102, 281, 623, 715, 929, 1311
BsaWI	WCCGGW	2 cut(s)	1643, 1817
BsaXI	ACNNNNNCTCC	8 cut(s)	1681, 1687, 1693, 1711, 1717, 1723, 1723, 1753
Bsc4I	CCNNNNNNNGG	5 cut(s)	265, 481, 1586, 1748, 1803
Bse118I	RCCGGY	1 cut(s)	1621
Bse1I	ACTGG	5 cut(s)	48, 287, 436, 1686, 1753
Bse3DI	GCAATG	2 cut(s)	773, 1224
Bse8I	GATNNNNATC	2 cut(s)	144, 1639
BseBI	CCWGG	3 cut(s)	624, 931, 1034
BseDI	CCNNGG	6 cut(s)	102, 281, 623, 715, 929, 1311
BseGI	GGATG	7 cut(s)	34, 255, 605, 711, 1050, 1225, 1345
BseJI	GATNNNNATC	2 cut(s)	144, 1639
BseLI	CCNNNNNNNGG	5 cut(s)	265, 481, 1586, 1748, 1803
BseMI	GCAATG	2 cut(s)	773, 1224
BseMII	CTCAG	6 cut(s)	846, 902, 1004, 1532, 1553, 1691
BseNI	ACTGG	5 cut(s)	48, 287, 436, 1686, 1753
BseRI	GAGGAG	3 cut(s)	98, 254, 1506
BseSI	GKGCMC	2 cut(s)	555, 1614
BseXI	GCAGC	5 cut(s)	319, 392, 912, 1093, 1492
Bsh1236I	CGCG	1 cut(s)	1362
Bsh1285I	CGRYCG	1 cut(s)	99
BshFI	GGCC	5 cut(s)	107, 254, 286, 760, 872
BsiEI	CGRYCG	1 cut(s)	99
BsiHKAI	GWGCWC	3 cut(s)	39, 555, 1320
BsiSI	CCGG	6 cut(s)	66, 117, 402, 1622, 1644, 1818
BsiWI	CGTACG	1 cut(s)	260
BslI	CCNNNNNNNGG	5 cut(s)	265, 481, 1586, 1748, 1803
BsmAI	GTCTC	2 cut(s)	1312, 1542
BsnI	GGCC	5 cut(s)	107, 254, 286, 760, 872
Bsp1286I	GDGCHC	4 cut(s)	39, 555, 1320, 1614
Bsp143I	GATC	8 cut(s)	171, 193, 484, 574, 700, 737, 835, 1499
BspACI	CCGC	5 cut(s)	99, 1404, 1407, 1563, 1807
BspANI	GGCC	5 cut(s)	107, 254, 286, 760, 872
BspCNI	CTCAG	6 cut(s)	845, 901, 1003, 1531, 1552, 1690
BspFNI	CGCG	1 cut(s)	1362
BspMAI	CTGCAG	1 cut(s)	1510
BspPI	GGATC	2 cut(s)	492, 830
BspTI	CTTAAG	2 cut(s)	565, 590
BsrDI	GCAATG	2 cut(s)	773, 1224
BsrFI	RCCGGY	1 cut(s)	1621
BsrI	ACTGG	5 cut(s)	48, 287, 436, 1686, 1753
BssAI	RCCGGY	1 cut(s)	1621
BssECI	CCNNGG	6 cut(s)	102, 281, 623, 715, 929, 1311
BssMI	GATC	8 cut(s)	171, 193, 484, 574, 700, 737, 835, 1499
BssSI	CACGAG	1 cut(s)	1110
BssT1I	CCWWGG	2 cut(s)	102, 715
Bst2BI	CACGAG	1 cut(s)	1110
Bst2UI	CCWGG	3 cut(s)	624, 931, 1034
Bst4CI	ACNGT	2 cut(s)	853, 1829
BstAFI	CTTAAG	2 cut(s)	565, 590
BstAPI	GCANNNNNTGC	2 cut(s)	53, 1262
BstDEI	CTNAG	7 cut(s)	668, 832, 888, 990, 1518, 1539, 1677
BstDSI	CCRYGG	1 cut(s)	1311
BstF5I	GGATG	7 cut(s)	34, 255, 605, 711, 1050, 1225, 1345
BstFNI	CGCG	1 cut(s)	1362
BstH2I	RGCGCY	1 cut(s)	1816
BstHHI	GCGC	2 cut(s)	380, 1815
BstKTI	GATC	8 cut(s)	174, 196, 487, 577, 703, 740, 838, 1502
BstMAI	GTCTC	2 cut(s)	1312, 1542
BstMBI	GATC	8 cut(s)	171, 193, 484, 574, 700, 737, 835, 1499
BstMCI	CGRYCG	1 cut(s)	99
BstMWI	GCNNNNNNNGC	6 cut(s)	53, 62, 113, 530, 931, 1262
BstNI	CCWGG	3 cut(s)	624, 931, 1034
BstNSI	RCATGY	2 cut(s)	643, 1177
BstSCI	CCNGG	5 cut(s)	116, 401, 622, 929, 1032
BstSFI	CTRYAG	1 cut(s)	1506
BstSLI	GKGCMC	2 cut(s)	555, 1614
BstUI	CGCG	1 cut(s)	1362
BstV1I	GCAGC	5 cut(s)	319, 392, 912, 1093, 1492
BstX2I	RGATCY	2 cut(s)	737, 835
BstXI	CCANNNNNNTGG	2 cut(s)	157, 1190
BstYI	RGATCY	2 cut(s)	737, 835
BsuI	GTATCC	1 cut(s)	1651
BsuRI	GGCC	5 cut(s)	107, 254, 286, 760, 872
BtgI	CCRYGG	1 cut(s)	1311
BtgZI	GCGATG	1 cut(s)	1346
BtrI	CACGTC	1 cut(s)	1180
BtsCI	GGATG	7 cut(s)	34, 255, 605, 711, 1050, 1225, 1345
BtsIMutI	CAGTG	4 cut(s)	41, 656, 858, 1746
CfoI	GCGC	2 cut(s)	380, 1815
Cfr10I	RCCGGY	1 cut(s)	1621
Cfr13I	GGNCC	3 cut(s)	512, 1030, 1324
Csp6I	GTAC	4 cut(s)	130, 261, 686, 1689
CviAII	CATG	8 cut(s)	328, 640, 899, 1019, 1082, 1168, 1174, 1321
CviQI	GTAC	4 cut(s)	130, 261, 686, 1689
DdeI	CTNAG	7 cut(s)	668, 832, 888, 990, 1518, 1539, 1677
DpnI	GATC	8 cut(s)	173, 195, 486, 576, 702, 739, 837, 1501
DpnII	GATC	8 cut(s)	171, 193, 484, 574, 700, 737, 835, 1499
DraI	TTTAAA	2 cut(s)	1003, 1066
DrdI	GACNNNNNNGTC	1 cut(s)	1178
DriI	GACNNNNNGTC	1 cut(s)	1126
DseDI	GACNNNNNNGTC	1 cut(s)	1178
EaeI	YGGCCR	3 cut(s)	252, 758, 870
Eam1105I	GACNNNNNGTC	1 cut(s)	1126
EciI	GGCGGA	1 cut(s)	1822
Ecl136II	GAGCTC	1 cut(s)	1318
Eco130I	CCWWGG	2 cut(s)	102, 715
Eco24I	GRGCYC	1 cut(s)	1320
Eco32I	GATATC	1 cut(s)	883
Eco47I	GGWCC	3 cut(s)	512, 1030, 1324
Eco53kI	GAGCTC	1 cut(s)	1318
EcoICRI	GAGCTC	1 cut(s)	1318
EcoRII	CCWGG	3 cut(s)	622, 929, 1032
EcoRV	GATATC	1 cut(s)	883
EcoT14I	CCWWGG	2 cut(s)	102, 715
EcoT38I	GRGCYC	1 cut(s)	1320
ErhI	CCWWGG	2 cut(s)	102, 715
FaeI	CATG	8 cut(s)	331, 643, 902, 1022, 1085, 1171, 1177, 1324
FatI	CATG	8 cut(s)	327, 639, 898, 1018, 1081, 1167, 1173, 1320
FbaI	TGATCA	1 cut(s)	574
Fnu4HI	GCNGC	7 cut(s)	333, 381, 926, 1107, 1405, 1506, 1563
FokI	GGATG	7 cut(s)	41, 242, 612, 718, 1057, 1232, 1352
FriOI	GRGCYC	1 cut(s)	1320
Fsp4HI	GCNGC	7 cut(s)	333, 381, 926, 1107, 1405, 1506, 1563
FspBI	CTAG	5 cut(s)	540, 683, 819, 1494, 1785
FspI	TGCGCA	1 cut(s)	379
GlaI	GCGC	2 cut(s)	379, 1814
GluI	GCNGC	7 cut(s)	333, 381, 926, 1107, 1405, 1506, 1563
GsuI	CTGGAG	1 cut(s)	1770
HaeII	RGCGCY	1 cut(s)	1816
HaeIII	GGCC	5 cut(s)	107, 254, 286, 760, 872
HapII	CCGG	6 cut(s)	66, 117, 402, 1622, 1644, 1818
HhaI	GCGC	2 cut(s)	380, 1815
Hin1II	CATG	8 cut(s)	331, 643, 902, 1022, 1085, 1171, 1177, 1324
Hin6I	GCGC	2 cut(s)	378, 1813
HinP1I	GCGC	2 cut(s)	378, 1813
HinfI	GANTC	7 cut(s)	74, 134, 181, 754, 826, 1364, 1463
HpaII	CCGG	6 cut(s)	66, 117, 402, 1622, 1644, 1818
HphI	GGTGA	6 cut(s)	869, 980, 1034, 1223, 1280, 1673
Hpy166II	GTNNAC	3 cut(s)	475, 553, 1147
Hpy188III	TCNNGA	7 cut(s)	158, 191, 438, 1118, 1368, 1546, 1785
Hpy8I	GTNNAC	3 cut(s)	475, 553, 1147
Hpy99I	CGWCG	2 cut(s)	113, 268
HpyAV	CCTTC	4 cut(s)	506, 646, 918, 1330
HpyCH4III	ACNGT	2 cut(s)	853, 1829
HpyCH4IV	ACGT	3 cut(s)	263, 1179, 1258
HpyCH4V	TGCA	9 cut(s)	553, 788, 1018, 1057, 1094, 1160, 1177, 1229, 1508
HpyF10VI	GCNNNNNNNGC	6 cut(s)	53, 62, 113, 530, 931, 1262
HpyF3I	CTNAG	7 cut(s)	668, 832, 888, 990, 1518, 1539, 1677
HpySE526I	ACGT	3 cut(s)	263, 1179, 1258
Hsp92II	CATG	8 cut(s)	331, 643, 902, 1022, 1085, 1171, 1177, 1324
HspAI	GCGC	2 cut(s)	378, 1813
Ksp22I	TGATCA	1 cut(s)	574
Kzo9I	GATC	8 cut(s)	171, 193, 484, 574, 700, 737, 835, 1499
LmnI	GCTCC	4 cut(s)	521, 1315, 1714, 1820
Lsp1109I	GCAGC	5 cut(s)	319, 392, 912, 1093, 1492
LweI	GCATC	3 cut(s)	56, 103, 1046
MaeI	CTAG	5 cut(s)	540, 683, 819, 1494, 1785
MaeII	ACGT	3 cut(s)	263, 1179, 1258
MaeIII	GTNAC	5 cut(s)	847, 945, 1006, 1022, 1510
MalI	GATC	8 cut(s)	173, 195, 486, 576, 702, 739, 837, 1501
MboI	GATC	8 cut(s)	171, 193, 484, 574, 700, 737, 835, 1499
MboII	GAAGA	4 cut(s)	181, 309, 314, 1467
MfeI	CAATTG	1 cut(s)	516
MflI	RGATCY	2 cut(s)	737, 835
MhlI	GDGCHC	4 cut(s)	39, 555, 1320, 1614
MlsI	TGGCCA	2 cut(s)	254, 872
MluNI	TGGCCA	2 cut(s)	254, 872
MlyI	GAGTC	2 cut(s)	68, 1472
MmeI	TCCRAC	2 cut(s)	105, 1736
Mox20I	TGGCCA	2 cut(s)	254, 872
MroXI	GAANNNNTTC	1 cut(s)	180
MscI	TGGCCA	2 cut(s)	254, 872
MslI	CAYNNNNRTG	2 cut(s)	464, 1172
Msp20I	TGGCCA	2 cut(s)	254, 872
MspCI	CTTAAG	2 cut(s)	565, 590
MspI	CCGG	6 cut(s)	66, 117, 402, 1622, 1644, 1818
MspR9I	CCNGG	5 cut(s)	118, 403, 624, 931, 1034
MunI	CAATTG	1 cut(s)	516
MvaI	CCWGG	3 cut(s)	624, 931, 1034
MvnI	CGCG	1 cut(s)	1362
MwoI	GCNNNNNNNGC	6 cut(s)	53, 62, 113, 530, 931, 1262
NciI	CCSGG	2 cut(s)	118, 403
NdeII	GATC	8 cut(s)	171, 193, 484, 574, 700, 737, 835, 1499
NlaIII	CATG	8 cut(s)	331, 643, 902, 1022, 1085, 1171, 1177, 1324
NmuCI	GTSAC	2 cut(s)	1006, 1022
NsbI	TGCGCA	1 cut(s)	379
NspI	RCATGY	2 cut(s)	643, 1177
PcsI	WCGNNNNNNNCGW	1 cut(s)	1420
PdmI	GAANNNNTTC	1 cut(s)	180
PfeI	GAWTC	5 cut(s)	134, 181, 754, 826, 1364
Pfl23II	CGTACG	1 cut(s)	260
PflMI	CCANNNNNTGG	1 cut(s)	481
PfoI	TCCNGGA	1 cut(s)	401
PkrI	GCNGC	7 cut(s)	334, 382, 927, 1108, 1406, 1507, 1564
PleI	GAGTC	2 cut(s)	68, 1471
PpsI	GAGTC	2 cut(s)	68, 1471
PshBI	ATTAAT	1 cut(s)	632
Psp124BI	GAGCTC	1 cut(s)	1320
Psp6I	CCWGG	3 cut(s)	622, 929, 1032
PspGI	CCWGG	3 cut(s)	622, 929, 1032
PspLI	CGTACG	1 cut(s)	260
PspPI	GGNCC	3 cut(s)	512, 1030, 1324
PstI	CTGCAG	1 cut(s)	1510
PsuI	RGATCY	2 cut(s)	737, 835
RsaI	GTAC	4 cut(s)	131, 262, 687, 1690
RsaNI	GTAC	4 cut(s)	130, 261, 686, 1689
RseI	CAYNNNNRTG	2 cut(s)	464, 1172
SacI	GAGCTC	1 cut(s)	1320
SatI	GCNGC	7 cut(s)	333, 381, 926, 1107, 1405, 1506, 1563
Sau3AI	GATC	8 cut(s)	171, 193, 484, 574, 700, 737, 835, 1499
Sau96I	GGNCC	3 cut(s)	512, 1030, 1324
ScaI	AGTACT	1 cut(s)	687
SchI	GAGTC	2 cut(s)	68, 1472
ScrFI	CCNGG	5 cut(s)	118, 403, 624, 931, 1034
SduI	GDGCHC	4 cut(s)	39, 555, 1320, 1614
SfaNI	GCATC	3 cut(s)	56, 103, 1046
SfcI	CTRYAG	1 cut(s)	1506
SinI	GGWCC	3 cut(s)	512, 1030, 1324
SmiI	ATTTAAAT	1 cut(s)	1066
SmiMI	CAYNNNNRTG	2 cut(s)	464, 1172
SmlI	CTYRAG	2 cut(s)	565, 590
SmoI	CTYRAG	2 cut(s)	565, 590
SsiI	CCGC	5 cut(s)	99, 1404, 1407, 1563, 1807
SspMI	CTAG	5 cut(s)	540, 683, 819, 1494, 1785
SstI	GAGCTC	1 cut(s)	1320
StyD4I	CCNGG	5 cut(s)	116, 401, 622, 929, 1032
StyI	CCWWGG	2 cut(s)	102, 715
SwaI	ATTTAAAT	1 cut(s)	1066
TaaI	ACNGT	2 cut(s)	853, 1829
TaiI	ACGT	3 cut(s)	266, 1182, 1261
TatI	WGTACW	2 cut(s)	685, 1688
TauI	GCSGC	2 cut(s)	1407, 1565
TfiI	GAWTC	5 cut(s)	134, 181, 754, 826, 1364
TscAI	CASTG	4 cut(s)	48, 663, 858, 1753
TseFI	GTSAC	2 cut(s)	1006, 1022
TseI	GCWGC	5 cut(s)	332, 380, 925, 1106, 1505
Tsp45I	GTSAC	2 cut(s)	1006, 1022
TspDTI	ATGAA	5 cut(s)	665, 887, 1349, 1467, 1557
TspGWI	ACGGA	3 cut(s)	248, 1328, 1475
TspRI	CASTG	4 cut(s)	48, 663, 858, 1753
Van91I	CCANNNNNTGG	1 cut(s)	481
Vha464I	CTTAAG	2 cut(s)	565, 590
VneI	GTGCAC	1 cut(s)	551
VpaK11BI	GGWCC	3 cut(s)	512, 1030, 1324
VspI	ATTAAT	1 cut(s)	632
XapI	RAATTY	3 cut(s)	1061, 1553, 1756
XbaI	TCTAGA	1 cut(s)	1784
XceI	RCATGY	2 cut(s)	643, 1177
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1318
XmnI	GAANNNNTTC	1 cut(s)	180
XspI	CTAG	5 cut(s)	540, 683, 819, 1494, 1785
ZrmI	AGTACT	1 cut(s)	687

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.