Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr3C

Orthogroup ID

OG0003007

Physical Location & Seq

Genomic Coordinates Reverse (-)

33598871 .. 33603925

Gene Structure 5055 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh3CG315700.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1989 bp

ATGTCGAAGAGGAAGTTCGGATTCGAAGGGTTCGGCATAAACCGGCAATCGACCTACAACTTCGAGCAGTCGCAACAAGACCCTCAGCGACTCTACGTCCCTCCGTCCTCCTCCTCCTCCCGCGCTCACGGCCACGACAATTACGAAGACACCGACCTTGACAACATCGATTACGACGACATTGAAGCCGGAAACACTCACGGTAACGGCGGAGGAGACGACGCCGGCGGCGGCGGCGGCGACGAAGAAGTCGATCCTCTCGATGCTTTCATGGAGGGGATTCACCAGGAGGTGAGGTCAGCTCCGCCGCCCAAGCCCAAGGAGAAGGCCGACAAGTACCAGGACGATGACGAGGAGGATCATCTGGAGAGTTTCTTGAGGGCCAAGAAGGACGCCACGCTGACTCTGGCCTCCGACGCGCTCCACGCCGGCTATGATTCCGACGAGGAGGTCTATGCCGCTGCCAAGGCTGTGGATGCCGGAATGTTGGAGTATGATTCCGATGACAATCCCGTTGTTGTGGACAGGAGGAAGATCGAGCCAATTTCAGCTCTGGATCATTCTTCCATTGACTACGAGCCATTCACTAAGGACTTCTACGAGGAAAAAGAGTCGATTTCAGGGATGAGTGAGCAGGATGTTGCCGAGTACCGGAAGAGCTTGGCCATCCGAGTGTCTGGCTTTGATGTACCAAGGCCAATTAAGATATTTGATGACTGTATGTTTTCTCCGCAACTCATGAATGCAATTAAAAAACAAGAGTATGAAAAGCCTACACCAATACAGTGCCAAGCTTTACCCATAGTTCTCTCTGGGAGGGATATCATTGGTATAGCAAAAACTGGCTCCGGAAAGACTGCCGCCTTTGTACTTCCCATGATTTGCCATATTATGGATCAGCCAGAACTTCAGAAAGAAGAAGGTCCTATAGGAGTCATATGTGCACCTACCAGAGAACTGGCGCAACAAATACACTTAGAGGCCAAGAAATTTGCTAAATCACATGGCATCCGTGTCTGCGCTGTATATGGTGGGATGTCGAAGCTTGATCAGTTCAAAGAACTTAAGGCAGGATGTGAGATAGTTGTAGCTACTCCTGGGAGATTGATAGACATGATTAAAATGAAGGCACTGACGATGCTACGTGTTACTTACCTAGTGCTTGATGAGGCTGATCGGATGTTTGACCTTGGTTTTGAGCCTCAAATTAGGTCTATAGTTGGTCAGATTAGGCCAGACCGGCAAACGCTACTCTTTTCTGCTACAATGCCACGTAAAGTTGAAAAGCTGGCTAGAGAAATTCTTTCTGACCCTGTAAGAGTTACAGTGGGTGAGGTGGGAATGGCCAATGAGGATATCACTCAAGTTGTTCATGTACTTCCTTCTGAAGCTGAGAAATTGCCATGGCTTCTTGAAAGATTACCTGGTATGATTGATGACGGTGATGTTCTCGTATTTGCTTCTAAAAAAGCTGCAGTGGATGAGATCGAAACACAGCTTTCTCAGAAAGGTTTTAAAGTTGCAGCTATTCATGGTGATAAGGACCAGGCTACTAGGATGGATATTATGCAAAAATATAAATCTGGCATCTACCATGTTCTGATTGCAACTGATGTTGCTGCTCGTGGTCTTAACATCAAGTCAATTAAGTCAGTTGTGAACTTTGATATTGCAAAGGACATGGACATGCATGTTCATCGTATTGGTAGAACAGGTCGTGCCGGTGATAAAGATGGGACTGCATACACTCTTATCACGCATAAAGAGGCACGGTTTGCTGGAGAATTGGTTAATAGCTTAGTTGGTGCTGGTCAGAATGTTCCCGTGGAGCTCATGGACCTTGCAATGAAGGTAAATGTGCGCTACTGTTCAAATATTCTTATAGTTTATATATTGAGCTTATGCATGTGTCCAATCGTGATGTATTTTAGTTTCGTACTGCTTGCTATCCGGACATATAAAAGTCGTCCAATTGTGTTATGTTTTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0006139 GO:0006725 GO:0006807 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0009987 GO:0010501 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0031974 GO:0031981 GO:0034641 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0070013 GO:0070035 GO:0071704 GO:0090304 GO:0140098 GO:1901360

KEGG Pathways

Metabolic & Signaling

ko03040 map03040

Pfam Domains

Protein Families

DEAD Helicase_C

Protein Analysis

662

Amino Acids

73.47

Weight (kDa)

5.18

Isoelectric Point (pI)

40.95

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DEAD	PF00270	259 - 429	3.2e-52	DEAD/DEAH box helicase
Helicase_C	PF00271	476 - 575	3.4e-26	Helicase conserved C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0003007)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G47330
fragaria_vesca	FvH4_1g27100 FvH4_1g27100 FvH4_1g27100
malus_domestica	MD00G1118300.v1.1 MD00G1118400.v1.1 MD09G1283400.v1.1 MD17G1274100.v1.1 MD17G1274800.v1.1 MD17G1275300.v1.1 MD17G1276500.v1.1
prunus_persica	Prupe.3G010900_v2.0.a1 Prupe.3G010900_v2.0.a1 Prupe.3G010900_v2.0.a1 Prupe.3G010900_v2.0.a1 Prupe.3G010900_v2.0.a1
pyrus_communis	pycom09g19130 pycom09g19140 pycom17g27210 pycom17g27330
rosa_chinensis	RchiOBHm_Chr3g0487421
rosa_laevigata	RLG00000023004
rosa_multiflora	Rmu_sc0008232.1_g000009 Rmu_sc0022533.1_g000001 Rmu_sc0031316.1_g000001
rosa_roxburghii	Rroxscaffold_6G00394790
rosa_rugosa	Rorug03G0234000
rosa_samantha	Rh2AG395100 Rh2CG381700 Rh3AG282000 Rh3BG317600 Rh3CG315700 Rh3DG313600 Rh4CG235900 Rh7AG337700 Rh7AG476200 Rh7CG355800
rosa_wichuraiana	Rw3G024970

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	248, 449
AccB7I	CCANNNNNTGG	1 cut(s)	892
AccII	CGCG	2 cut(s)	123, 419
AccIII	TCCGGA	2 cut(s)	848, 1950
AclWI	GGATC	4 cut(s)	248, 366, 564, 903
AcoI	YGGCCR	3 cut(s)	130, 663, 1344
AcsI	RAATTY	2 cut(s)	989, 1299
AcuI	CTGAAG	2 cut(s)	893, 1407
AcyI	GRCGYC	2 cut(s)	222, 393
AfaI	GTAC	6 cut(s)	338, 650, 690, 870, 1377, 1938
AfiI	CCNNNNNNNGG	2 cut(s)	651, 892
AflII	CTTAAG	1 cut(s)	1064
AflIII	ACRYGT	1 cut(s)	1144
AgsI	TTSAA	5 cut(s)	185, 1057, 1283, 1415, 1872
AhdI	GACNNNNNGTC	1 cut(s)	95
AjnI	CCWGG	5 cut(s)	285, 339, 1096, 1423, 1545
Alw21I	GWGCWC	2 cut(s)	946, 1833
Alw26I	GTCTC	1 cut(s)	210
Alw44I	GTGCAC	1 cut(s)	942
AlwI	GGATC	4 cut(s)	248, 366, 564, 903
Aor13HI	TCCGGA	2 cut(s)	848, 1950
AoxI	GGCC	9 cut(s)	130, 327, 381, 408, 663, 695, 981, 1232, 1344
ApaLI	GTGCAC	1 cut(s)	942
ApeKI	GCWGC	4 cut(s)	461, 1472, 1523, 1619
ApoI	RAATTY	2 cut(s)	989, 1299
ArsI	GACNNNNNNTTYG	2 cut(s)	1178, 1210
AspLEI	GCGC	5 cut(s)	125, 421, 964, 1022, 1863
AspS9I	GGNCC	4 cut(s)	381, 923, 1543, 1837
AsuHPI	GGTGA	6 cut(s)	275, 304, 1343, 1454, 1547, 1736
AsuII	TTCGAA	1 cut(s)	24
AvaII	GGWCC	3 cut(s)	923, 1543, 1837
BaeGI	GKGCMC	1 cut(s)	946
BalI	TGGCCA	2 cut(s)	665, 1346
BanII	GRGCYC	1 cut(s)	1833
BauI	CACGAG	1 cut(s)	1623
BbsI	GAAGAC	1 cut(s)	153
Bbv12I	GWGCWC	2 cut(s)	946, 1833
BbvCI	CCTCAGC	1 cut(s)	84
BbvI	GCAGC	4 cut(s)	448, 1459, 1535, 1606
BccI	CCATC	3 cut(s)	674, 1552, 1727
BceAI	ACGGC	2 cut(s)	145, 223
BcgI	CGANNNNNNTGC	2 cut(s)	1679, 1713
BciT130I	CCWGG	5 cut(s)	287, 341, 1098, 1425, 1547
BclI	TGATCA	1 cut(s)	1048
BcoDI	GTCTC	1 cut(s)	210
BfaI	CTAG	3 cut(s)	1157, 1293, 1554
BfmI	CTRYAG	3 cut(s)	927, 1215, 1473
BfrI	CTTAAG	1 cut(s)	1064
BglI	GCCNNNNNGGC	1 cut(s)	1240
Bme1390I	CCNGG	5 cut(s)	287, 341, 1098, 1425, 1547
Bme18I	GGWCC	3 cut(s)	923, 1543, 1837
BmeRI	GACNNNNNGTC	1 cut(s)	95
BmgT120I	GGNCC	4 cut(s)	381, 923, 1543, 1837
BmiI	GGNNCC	1 cut(s)	847
BmrFI	CCNGG	5 cut(s)	287, 341, 1098, 1425, 1547
BmsI	GCATC	5 cut(s)	253, 466, 1017, 1128, 1596
BpiI	GAAGAC	1 cut(s)	153
BplI	GAGNNNNNCTC	2 cut(s)	286, 318
BpmI	CTGGAG	2 cut(s)	386, 1800
Bpu10I	CCTNAGC	1 cut(s)	84
Bpu14I	TTCGAA	1 cut(s)	24
BpuEI	CTTGAG	2 cut(s)	397, 1347
Bsa29I	ATCGAT	1 cut(s)	168
BsaAI	YACGTR	2 cut(s)	1145, 1274
BsaBI	GATNNNNATC	1 cut(s)	507
BsaHI	GRCGYC	2 cut(s)	222, 393
BsaJI	CCNNGG	7 cut(s)	318, 465, 692, 1097, 1189, 1403, 1824
BsaWI	WCCGGW	3 cut(s)	651, 848, 1950
BsaXI	ACNNNNNCTCC	4 cut(s)	281, 311, 1773, 1803
Bsc4I	CCNNNNNNNGG	2 cut(s)	651, 892
Bse118I	RCCGGY	5 cut(s)	42, 224, 428, 1239, 1721
Bse1I	ACTGG	2 cut(s)	847, 963
Bse3DI	GCAATG	1 cut(s)	1851
Bse8I	GATNNNNATC	1 cut(s)	507
BseAI	TCCGGA	2 cut(s)	848, 1950
BseBI	CCWGG	5 cut(s)	287, 341, 1098, 1425, 1547
BseCI	ATCGAT	1 cut(s)	168
BseDI	CCNNGG	7 cut(s)	318, 465, 692, 1097, 1189, 1403, 1824
BseJI	GATNNNNATC	1 cut(s)	507
BseLI	CCNNNNNNNGG	2 cut(s)	651, 892
BseMI	GCAATG	1 cut(s)	1851
BseMII	CTCAG	3 cut(s)	98, 1383, 1517
BseNI	ACTGG	2 cut(s)	847, 963
BseRI	GAGGAG	6 cut(s)	100, 103, 106, 228, 368, 461
BseSI	GKGCMC	1 cut(s)	946
BseXI	GCAGC	4 cut(s)	448, 1459, 1535, 1606
Bsh1236I	CGCG	2 cut(s)	123, 419
BshFI	GGCC	9 cut(s)	132, 329, 383, 410, 665, 697, 983, 1234, 1346
BshVI	ATCGAT	1 cut(s)	168
BsiHKAI	GWGCWC	2 cut(s)	946, 1833
BslFI	GGGAC	2 cut(s)	83, 1750
BslI	CCNNNNNNNGG	2 cut(s)	651, 892
BsmAI	GTCTC	1 cut(s)	210
BsmBI	CGTCTC	1 cut(s)	210
BsmFI	GGGAC	2 cut(s)	83, 1750
BsmI	GAATGC	1 cut(s)	748
BsnI	GGCC	9 cut(s)	132, 329, 383, 410, 665, 697, 983, 1234, 1346
Bsp119I	TTCGAA	1 cut(s)	24
Bsp1286I	GDGCHC	2 cut(s)	946, 1833
Bsp13I	TCCGGA	2 cut(s)	848, 1950
Bsp143I	GATC	8 cut(s)	253, 358, 534, 556, 895, 1048, 1174, 1485
Bsp19I	CCATGG	1 cut(s)	1403
BspANI	GGCC	9 cut(s)	132, 329, 383, 410, 665, 697, 983, 1234, 1346
BspCNI	CTCAG	3 cut(s)	97, 1384, 1516
BspDI	ATCGAT	1 cut(s)	168
BspEI	TCCGGA	2 cut(s)	848, 1950
BspFNI	CGCG	2 cut(s)	123, 419
BspHI	TCATGA	1 cut(s)	738
BspLI	GGNNCC	1 cut(s)	847
BspMAI	CTGCAG	1 cut(s)	1477
BspPI	GGATC	4 cut(s)	248, 366, 564, 903
BspQI	GCTCTTC	1 cut(s)	650
BspT104I	TTCGAA	1 cut(s)	24
BspTI	CTTAAG	1 cut(s)	1064
BsrDI	GCAATG	1 cut(s)	1851
BsrFI	RCCGGY	5 cut(s)	42, 224, 428, 1239, 1721
BsrI	ACTGG	2 cut(s)	847, 963
BssAI	RCCGGY	5 cut(s)	42, 224, 428, 1239, 1721
BssECI	CCNNGG	7 cut(s)	318, 465, 692, 1097, 1189, 1403, 1824
BssMI	GATC	8 cut(s)	253, 358, 534, 556, 895, 1048, 1174, 1485
BssNI	GRCGYC	2 cut(s)	222, 393
BssSI	CACGAG	1 cut(s)	1623
BssT1I	CCWWGG	5 cut(s)	318, 465, 692, 1189, 1403
Bst2BI	CACGAG	1 cut(s)	1623
Bst2UI	CCWGG	5 cut(s)	287, 341, 1098, 1425, 1547
Bst4CI	ACNGT	7 cut(s)	203, 719, 786, 1327, 1442, 1773, 1868
Bst6I	CTCTTC	2 cut(s)	2, 650
BstACI	GRCGYC	2 cut(s)	222, 393
BstAFI	CTTAAG	1 cut(s)	1064
BstAPI	GCANNNNNTGC	1 cut(s)	1775
BstBAI	YACGTR	2 cut(s)	1145, 1274
BstBI	TTCGAA	1 cut(s)	24
BstC8I	GCNNGC	4 cut(s)	226, 430, 1290, 1944
BstDEI	CTNAG	6 cut(s)	84, 588, 976, 1392, 1503, 1798
BstDSI	CCRYGG	2 cut(s)	1403, 1824
BstFNI	CGCG	2 cut(s)	123, 419
BstHHI	GCGC	5 cut(s)	125, 421, 964, 1022, 1863
BstKTI	GATC	8 cut(s)	256, 361, 537, 559, 898, 1051, 1177, 1488
BstMAI	GTCTC	1 cut(s)	210
BstMBI	GATC	8 cut(s)	253, 358, 534, 556, 895, 1048, 1174, 1485
BstNI	CCWGG	5 cut(s)	287, 341, 1098, 1425, 1547
BstNSI	RCATGY	3 cut(s)	1690, 1694, 1909
BstSCI	CCNGG	5 cut(s)	285, 339, 1096, 1423, 1545
BstSFI	CTRYAG	3 cut(s)	927, 1215, 1473
BstSLI	GKGCMC	1 cut(s)	946
BstUI	CGCG	2 cut(s)	123, 419
BstV1I	GCAGC	4 cut(s)	448, 1459, 1535, 1606
BstV2I	GAAGAC	1 cut(s)	153
BstXI	CCANNNNNNTGG	2 cut(s)	472, 958
Bsu15I	ATCGAT	1 cut(s)	168
BsuRI	GGCC	9 cut(s)	132, 329, 383, 410, 665, 697, 983, 1234, 1346
BsuTUI	ATCGAT	1 cut(s)	168
BtgI	CCRYGG	2 cut(s)	1403, 1824
BtsI	GCAGTG	1 cut(s)	1482
BtsIMutI	CAGTG	4 cut(s)	791, 1130, 1332, 1482
Cac8I	GCNNGC	4 cut(s)	226, 430, 1290, 1944
CciI	TCATGA	1 cut(s)	738
CfoI	GCGC	5 cut(s)	125, 421, 964, 1022, 1863
Cfr10I	RCCGGY	5 cut(s)	42, 224, 428, 1239, 1721
Cfr13I	GGNCC	4 cut(s)	381, 923, 1543, 1837
ClaI	ATCGAT	1 cut(s)	168
CseI	GACGC	3 cut(s)	230, 401, 425
CsiI	ACCWGGT	1 cut(s)	1423
Csp6I	GTAC	6 cut(s)	337, 649, 689, 869, 1376, 1937
CviQI	GTAC	6 cut(s)	337, 649, 689, 869, 1376, 1937
DdeI	CTNAG	6 cut(s)	84, 588, 976, 1392, 1503, 1798
DpnI	GATC	8 cut(s)	255, 360, 536, 558, 897, 1050, 1176, 1487
DpnII	GATC	8 cut(s)	253, 358, 534, 556, 895, 1048, 1174, 1485
DraI	TTTAAA	1 cut(s)	1516
DrdI	GACNNNNNNGTC	2 cut(s)	248, 449
DriI	GACNNNNNGTC	1 cut(s)	95
DseDI	GACNNNNNNGTC	2 cut(s)	248, 449
EaeI	YGGCCR	3 cut(s)	130, 663, 1344
Eam1104I	CTCTTC	2 cut(s)	2, 650
Eam1105I	GACNNNNNGTC	1 cut(s)	95
EarI	CTCTTC	2 cut(s)	2, 650
EciI	GGCGGA	2 cut(s)	225, 294
Ecl136II	GAGCTC	1 cut(s)	1831
Eco130I	CCWWGG	5 cut(s)	318, 465, 692, 1189, 1403
Eco24I	GRGCYC	1 cut(s)	1833
Eco32I	GATATC	2 cut(s)	823, 1357
Eco47I	GGWCC	3 cut(s)	923, 1543, 1837
Eco53kI	GAGCTC	1 cut(s)	1831
Eco57I	CTGAAG	2 cut(s)	893, 1407
EcoICRI	GAGCTC	1 cut(s)	1831
EcoO109I	RGGNCCY	1 cut(s)	923
EcoRII	CCWGG	5 cut(s)	285, 339, 1096, 1423, 1545
EcoRV	GATATC	2 cut(s)	823, 1357
EcoT14I	CCWWGG	5 cut(s)	318, 465, 692, 1189, 1403
EcoT22I	ATGCAT	2 cut(s)	1692, 1907
EcoT38I	GRGCYC	1 cut(s)	1833
ErhI	CCWWGG	5 cut(s)	318, 465, 692, 1189, 1403
Esp3I	CGTCTC	1 cut(s)	210
FaqI	GGGAC	2 cut(s)	83, 1750
FauI	CCCGC	1 cut(s)	128
FauNDI	CATATG	1 cut(s)	938
FbaI	TGATCA	1 cut(s)	1048
FriOI	GRGCYC	1 cut(s)	1833
FspBI	CTAG	3 cut(s)	1157, 1293, 1554
GlaI	GCGC	5 cut(s)	124, 420, 963, 1021, 1862
GsuI	CTGGAG	2 cut(s)	386, 1800
HaeIII	GGCC	9 cut(s)	132, 329, 383, 410, 665, 697, 983, 1234, 1346
HgaI	GACGC	3 cut(s)	230, 401, 425
HhaI	GCGC	5 cut(s)	125, 421, 964, 1022, 1863
Hin1I	GRCGYC	2 cut(s)	222, 393
Hin6I	GCGC	5 cut(s)	123, 419, 962, 1020, 1861
HinP1I	GCGC	5 cut(s)	123, 419, 962, 1020, 1861
HindIII	AAGCTT	2 cut(s)	792, 1043
HinfI	GANTC	8 cut(s)	21, 90, 280, 403, 437, 497, 611, 933
HphI	GGTGA	6 cut(s)	275, 304, 1343, 1454, 1547, 1736
Hpy166II	GTNNAC	3 cut(s)	523, 944, 1660
Hpy188III	TCNNGA	9 cut(s)	260, 365, 376, 554, 739, 849, 1412, 1918, 1951
Hpy8I	GTNNAC	3 cut(s)	523, 944, 1660
Hpy99I	CGWCG	5 cut(s)	179, 224, 245, 419, 446
HpyAV	CCTTC	7 cut(s)	20, 319, 382, 914, 1120, 1392, 1843
HpyCH4III	ACNGT	7 cut(s)	203, 719, 786, 1327, 1442, 1773, 1868
HpyCH4IV	ACGT	3 cut(s)	96, 1144, 1273
HpyF3I	CTNAG	6 cut(s)	84, 588, 976, 1392, 1503, 1798
HpySE526I	ACGT	3 cut(s)	96, 1144, 1273
Hsp92I	GRCGYC	2 cut(s)	222, 393
HspAI	GCGC	5 cut(s)	123, 419, 962, 1020, 1861
Kpn2I	TCCGGA	2 cut(s)	848, 1950
KroI	GCCGGC	2 cut(s)	224, 428
KroNI	GCCGGC	2 cut(s)	226, 430
Ksp22I	TGATCA	1 cut(s)	1048
Kzo9I	GATC	8 cut(s)	253, 358, 534, 556, 895, 1048, 1174, 1485
LguI	GCTCTTC	1 cut(s)	650
LmnI	GCTCC	4 cut(s)	307, 426, 851, 1828
Lsp1109I	GCAGC	4 cut(s)	448, 1459, 1535, 1606
LweI	GCATC	5 cut(s)	253, 466, 1017, 1128, 1596
MabI	ACCWGGT	1 cut(s)	1423
MaeI	CTAG	3 cut(s)	1157, 1293, 1554
MaeII	ACGT	3 cut(s)	96, 1144, 1273
MaeIII	GTNAC	3 cut(s)	203, 1147, 1321
MalI	GATC	8 cut(s)	255, 360, 536, 558, 897, 1050, 1176, 1487
MboI	GATC	8 cut(s)	253, 358, 534, 556, 895, 1048, 1174, 1485
MboII	GAAGA	7 cut(s)	19, 158, 257, 544, 555, 667, 929
MfeI	CAATTG	1 cut(s)	1971
MhlI	GDGCHC	2 cut(s)	946, 1833
MlsI	TGGCCA	2 cut(s)	665, 1346
MluNI	TGGCCA	2 cut(s)	665, 1346
MlyI	GAGTC	4 cut(s)	84, 397, 620, 942
MmeI	TCCRAC	3 cut(s)	438, 465, 468
Mox20I	TGGCCA	2 cut(s)	665, 1346
Mph1103I	ATGCAT	2 cut(s)	1692, 1907
MreI	CGCCGGCG	1 cut(s)	224
MroI	TCCGGA	2 cut(s)	848, 1950
MroNI	GCCGGC	2 cut(s)	224, 428
MscI	TGGCCA	2 cut(s)	665, 1346
MseI	TTAA	8 cut(s)	702, 750, 1065, 1119, 1515, 1632, 1647, 1791
MslI	CAYNNNNRTG	2 cut(s)	671, 1685
Msp20I	TGGCCA	2 cut(s)	665, 1346
MspA1I	CMGCKG	1 cut(s)	461
MspCI	CTTAAG	1 cut(s)	1064
MspR9I	CCNGG	5 cut(s)	287, 341, 1098, 1425, 1547
MunI	CAATTG	1 cut(s)	1971
Mva1269I	GAATGC	1 cut(s)	748
MvaI	CCWGG	5 cut(s)	287, 341, 1098, 1425, 1547
MvnI	CGCG	2 cut(s)	123, 419
NaeI	GCCGGC	2 cut(s)	226, 430
NcoI	CCATGG	1 cut(s)	1403
NdeI	CATATG	1 cut(s)	938
NdeII	GATC	8 cut(s)	253, 358, 534, 556, 895, 1048, 1174, 1485
NgoMIV	GCCGGC	2 cut(s)	224, 428
NlaIV	GGNNCC	1 cut(s)	847
NmeAIII	GCCGAG	1 cut(s)	670
NsiI	ATGCAT	2 cut(s)	1692, 1907
NspI	RCATGY	3 cut(s)	1690, 1694, 1909
NspV	TTCGAA	1 cut(s)	24
PagI	TCATGA	1 cut(s)	738
PciSI	GCTCTTC	1 cut(s)	650
PcsI	WCGNNNNNNNCGW	5 cut(s)	141, 150, 174, 249, 258
PctI	GAATGC	1 cut(s)	748
PdiI	GCCGGC	2 cut(s)	226, 430
PfeI	GAWTC	4 cut(s)	21, 280, 437, 497
PflMI	CCANNNNNTGG	1 cut(s)	892
PleI	GAGTC	4 cut(s)	84, 397, 619, 941
PpsI	GAGTC	4 cut(s)	84, 397, 619, 941
Ppu21I	YACGTR	2 cut(s)	1145, 1274
PpuMI	RGGWCCY	1 cut(s)	923
Psp124BI	GAGCTC	1 cut(s)	1833
Psp5II	RGGWCCY	1 cut(s)	923
Psp6I	CCWGG	5 cut(s)	285, 339, 1096, 1423, 1545
PspGI	CCWGG	5 cut(s)	285, 339, 1096, 1423, 1545
PspN4I	GGNNCC	1 cut(s)	847
PspPI	GGNCC	4 cut(s)	381, 923, 1543, 1837
PspPPI	RGGWCCY	1 cut(s)	923
PstI	CTGCAG	1 cut(s)	1477
RsaI	GTAC	6 cut(s)	338, 650, 690, 870, 1377, 1938
RsaNI	GTAC	6 cut(s)	337, 649, 689, 869, 1376, 1937
RseI	CAYNNNNRTG	2 cut(s)	671, 1685
SacI	GAGCTC	1 cut(s)	1833
SapI	GCTCTTC	1 cut(s)	650
SaqAI	TTAA	8 cut(s)	702, 750, 1065, 1119, 1515, 1632, 1647, 1791
Sau3AI	GATC	8 cut(s)	253, 358, 534, 556, 895, 1048, 1174, 1485
Sau96I	GGNCC	4 cut(s)	381, 923, 1543, 1837
SchI	GAGTC	4 cut(s)	84, 397, 620, 942
ScrFI	CCNGG	5 cut(s)	287, 341, 1098, 1425, 1547
SduI	GDGCHC	2 cut(s)	946, 1833
SexAI	ACCWGGT	1 cut(s)	1423
SfaNI	GCATC	5 cut(s)	253, 466, 1017, 1128, 1596
SfcI	CTRYAG	3 cut(s)	927, 1215, 1473
SfuI	TTCGAA	1 cut(s)	24
SgrAI	CRCCGGYG	1 cut(s)	224
SinI	GGWCC	3 cut(s)	923, 1543, 1837
SmiMI	CAYNNNNRTG	2 cut(s)	671, 1685
SmlI	CTYRAG	3 cut(s)	376, 1064, 1362
SmoI	CTYRAG	3 cut(s)	376, 1064, 1362
SspI	AATATT	1 cut(s)	1876
SspMI	CTAG	3 cut(s)	1157, 1293, 1554
SstI	GAGCTC	1 cut(s)	1833
StyD4I	CCNGG	5 cut(s)	285, 339, 1096, 1423, 1545
StyI	CCWWGG	5 cut(s)	318, 465, 692, 1189, 1403
TaaI	ACNGT	7 cut(s)	203, 719, 786, 1327, 1442, 1773, 1868
TaiI	ACGT	3 cut(s)	99, 1147, 1276
TatI	WGTACW	2 cut(s)	868, 1375
TauI	GCSGC	7 cut(s)	231, 234, 237, 240, 310, 461, 863
TfiI	GAWTC	4 cut(s)	21, 280, 437, 497
Tru1I	TTAA	8 cut(s)	702, 750, 1065, 1119, 1515, 1632, 1647, 1791
Tru9I	TTAA	8 cut(s)	702, 750, 1065, 1119, 1515, 1632, 1647, 1791
TscAI	CASTG	4 cut(s)	791, 1137, 1332, 1482
TseI	GCWGC	4 cut(s)	461, 1472, 1523, 1619
TspDTI	ATGAA	8 cut(s)	259, 755, 780, 1139, 1361, 1520, 1685, 1862
TspGWI	ACGGA	2 cut(s)	93, 1001
TspRI	CASTG	4 cut(s)	791, 1137, 1332, 1482
Van91I	CCANNNNNTGG	1 cut(s)	892
Vha464I	CTTAAG	1 cut(s)	1064
VneI	GTGCAC	1 cut(s)	942
VpaK11BI	GGWCC	3 cut(s)	923, 1543, 1837
XapI	RAATTY	2 cut(s)	989, 1299
XceI	RCATGY	3 cut(s)	1690, 1694, 1909
XcmI	CCANNNNNNNNNTGG	1 cut(s)	403
XspI	CTAG	3 cut(s)	1157, 1293, 1554
Zsp2I	ATGCAT	2 cut(s)	1692, 1907

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.