Basic Information

Type: gene

Biological Identity

Species

rosa_chinensis

Chromosome

Orthogroup ID

OG0003007

Physical Location & Seq

Genomic Coordinates Reverse (-)

34619464 .. 34623880

Gene Structure 4417 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

PRQ45185

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2367 bp

ATGTCGAAGAGGAAGTTCGGATTCGAAGGGTTCGGCATAAACCGGCAATCGACCTACAACTTCGAGCAGTCGCAACAAGACCCTCAGCGACTCTACGTCCCTCCTTCCTCCTCCTCCTCCTCCCGCGCTCACGGCCACGACAATTACGAAGACACCGACCTTGACAACATCGATTACGACGACATTGAAGCCGGAAACACTCACGGTAACGGCGGAGGAGACGACGCCGGCGGCGGCGGCGGCGGTGACGAAGAAGTCGATCCTCTCGATGCTTTCATGGAGGGGATTCACCAGGAGGTGAGGTCAGCTCCGCCGCCCAAGCCCAAGGAGAAGGCCGACAAGTACCAGGACGATGACGAGGAGGATCATCTGGAGAGTTTCTTGAGGGCCAAGAAGGACGCCACGCTGACTCTGGCCTCCGACGCGCTCCACGCCGGCTATGATTCCGACGAGGAGGTCTATGCCGCTGCCAAGGCTGTGGATGCCGGAATGTTGGAGTATGATTCCGAGGACAATCCCGTTGTTGTGGACAGGAGGAAGATCGAGCCAATTTCAGCTCTGGATCATACTTCCATTGACTACGAGCCATTCACCAAGGACTTCTACGAGGAAAAAGAGTCGATTTCAGGGATGAGTGAGCAGGATGTTGCCGAGTACCGGAAGAGCTTGGCCATCCGAGTGTCTGGCTTTGATGTACCAAGGCCAATTAAGATATTTGATGACTGTGCGTTTTCTCCGCAACTCATGAATGCAATTAAAAAACAAGAGTACGAAAAGCCTACACCAATACAGTGCCAAGCTTTACCCATAGTTCTTTCTGGGAGGGATATCATTGGTATAGCAAAAACTGGCTCCGGAAAGACTGCCGCCTTTGTACTTCCCATGATTTGCCATATTATGGATCAGCCAGAACTTCAGAAAGAAGAAGGTCCTATAGGAGTCATATGTGCACCTACCAGAGAACTGGCACAACAAATACACTTAGAGGCCAAGAAATTTGCTAAATCACATGGCATCCGTGTCTGTGCTGTATATGGTGGGATGTCAAAGCTTGATCAGTTCAAAGAACTTAAGGCAGGATGCGAGATAGTTGTAGCTACTCCTGGGAGATTGATAGACATGATTAAAATGAAGGCACTGACGATGCTACGTGTAACTTACCTAGTGCTTGATGAGGCTGATCGGATGTTTGACCTTGGTTTTGAGCCTCAAATTAGGTCTATAGTTGGTCAGATTAGGCCAGACCGGCAAACGCTACTCTTTTCTGCTACAATGCCACGTAAAGTTGAAAAGCTGGCTAGAGAAATTCTTTCTGACCCTGTAAGAGTTACAGTGGGTGAGGTGGGAATGGCCAATGAGGATATCACTCAAGTTGTTCATGTACTTCCTTCTGAAGCTGAGAAATTGCCGTGGCTTCTTGAAAGATTACCTGGTATGATTGATGACGGTGATGTTCTCATATTTGCTTCTAAAAAAGCTGCAGTGGATGAGATCGAAACACAGCTTTCTCAGAAAGGTTTTAAAGTTGCAGCTATTCATGGTGATAAGGACCAGGCTACTAGGATGGATATTATGCAAAAATATAAATCTGGCATCTACCATGTTCTGATTGCAACTGATGTTGCTGCTCGTGGTCTTAACATCAAGTCAATTAAGTCAGTTGTGAACTTTGATATTGCAAAGGACATGGACATGCATGTTCATCGTATTGGTAGAACAGGTCGTGCCGGTGATAAAGACGGGACTGCATACACTCTTATCATGCATAAAGAGGCACGGTTTGCTGGAGAATTGGTTAATAGCTTAGTTGGTGCTGGTCAGAATGTTCCCGTGGAGCTCATGGACCTTGCAATGAAGGATGGAAGATTCAGGTCTAAACGTGATTCAAGAAAAGGAGGTGGAAAGAAGGGTAGGGGGAGAGGTGGTGGTGGAGGCGGTGGCGGCAGTGGTCGAGGTGTGCGCGGGGTGGATTTTGGTCTGGGTATTGGATATAATCCGGAATCCAATAGTTCTCCATCACCTGCTGTTACTAGTCGATCTGCCGCAGTTACTTCTCTAAGAACAGGAATGATGTCTCAATTCAAGAGTAAGTTTGTTTCTGCTTCATCAAACTCTCCAAGTCAGGGTTCTGGTAACAGTTCGAGTGCGCATGCCAACAAGAGGCCTGCATTACGAGGATTTGTAGCCGGTGGTTCTATTGGTGGAGACATACATAGATCTCAGACAACCAATACAGTTTCTCCAGCTCCTGCACCAGTGGCAAACATATCTTCTCAGAATTCTGGAGAAAATTCGAGCCAGAAGCCGACGGAAAGTTCTAGAGATAAACCTAGAGAAAGGCGGAGGCGCTCTGGTTGGGACTGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0006139 GO:0006725 GO:0006807 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0009987 GO:0010501 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0031974 GO:0031981 GO:0034641 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0070013 GO:0070035 GO:0071704 GO:0090304 GO:0140098 GO:1901360

KEGG Pathways

Metabolic & Signaling

ko03040 map03040

Pfam Domains

Protein Families

DEAD Helicase_C

Protein Analysis

788

Amino Acids

85.63

Weight (kDa)

5.98

Isoelectric Point (pI)

50.66

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DEAD	PF00270	261 - 431	4.3e-52	DEAD/DEAH box helicase
Helicase_C	PF00271	478 - 577	3.8e-26	Helicase conserved C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003007)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G47330
fragaria_vesca	FvH4_1g27100 FvH4_1g27100 FvH4_1g27100
malus_domestica	MD00G1118300.v1.1 MD00G1118400.v1.1 MD09G1283400.v1.1 MD17G1274100.v1.1 MD17G1274800.v1.1 MD17G1275300.v1.1 MD17G1276500.v1.1
prunus_persica	Prupe.3G010900_v2.0.a1 Prupe.3G010900_v2.0.a1 Prupe.3G010900_v2.0.a1 Prupe.3G010900_v2.0.a1 Prupe.3G010900_v2.0.a1
pyrus_communis	pycom09g19130 pycom09g19140 pycom17g27210 pycom17g27330
rosa_chinensis	RchiOBHm_Chr3g0487421
rosa_laevigata	RLG00000023004
rosa_multiflora	Rmu_sc0008232.1_g000009 Rmu_sc0022533.1_g000001 Rmu_sc0031316.1_g000001
rosa_roxburghii	Rroxscaffold_6G00394790
rosa_rugosa	Rorug03G0234000
rosa_samantha	Rh2AG395100 Rh2CG381700 Rh3AG282000 Rh3BG317600 Rh3CG315700 Rh3DG313600 Rh4CG235900 Rh7AG337700 Rh7AG476200 Rh7CG355800
rosa_wichuraiana	Rw3G024970

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	2029
AasI	GACNNNNNNGTC	2 cut(s)	254, 455
Acc16I	TGCGCA	1 cut(s)	2148
Acc36I	ACCTGC	1 cut(s)	2029
AccB7I	CCANNNNNTGG	1 cut(s)	898
AccII	CGCG	3 cut(s)	126, 425, 1962
AccIII	TCCGGA	2 cut(s)	854, 1996
AclWI	GGATC	4 cut(s)	254, 372, 570, 909
AcoI	YGGCCR	3 cut(s)	133, 669, 1350
AcsI	RAATTY	4 cut(s)	995, 1305, 2278, 2290
AcuI	CTGAAG	2 cut(s)	899, 1413
AcyI	GRCGYC	2 cut(s)	225, 399
AfaI	GTAC	6 cut(s)	344, 656, 696, 770, 876, 1383
AfiI	CCNNNNNNNGG	4 cut(s)	657, 898, 2123, 2160
AflII	CTTAAG	1 cut(s)	1070
AflIII	ACRYGT	1 cut(s)	1150
AgsI	TTSAA	6 cut(s)	188, 1063, 1289, 1421, 1887, 2083
AhdI	GACNNNNNGTC	1 cut(s)	95
AhlI	ACTAGT	1 cut(s)	2030
AjnI	CCWGG	5 cut(s)	291, 345, 1102, 1429, 1551
AjuI	GAANNNNNNNTTGG	2 cut(s)	2110, 2142
Alw21I	GWGCWC	2 cut(s)	952, 1839
Alw26I	GTCTC	3 cut(s)	213, 2079, 2199
Alw44I	GTGCAC	1 cut(s)	948
AlwI	GGATC	4 cut(s)	254, 372, 570, 909
AlwNI	CAGNNNCTG	1 cut(s)	2249
Aor13HI	TCCGGA	2 cut(s)	854, 1996
ApaLI	GTGCAC	1 cut(s)	948
ApeKI	GCWGC	4 cut(s)	467, 1478, 1529, 1625
ApoI	RAATTY	4 cut(s)	995, 1305, 2278, 2290
ArsI	GACNNNNNNTTYG	2 cut(s)	1184, 1216
AspLEI	GCGC	5 cut(s)	128, 427, 1962, 2149, 2349
AspS9I	GGNCC	4 cut(s)	387, 929, 1549, 1843
AsuHPI	GGTGA	9 cut(s)	257, 281, 310, 583, 1349, 1460, 1553, 1742, 2010
AsuII	TTCGAA	1 cut(s)	24
AvaII	GGWCC	3 cut(s)	929, 1549, 1843
BaeGI	GKGCMC	1 cut(s)	952
BalI	TGGCCA	2 cut(s)	671, 1352
BanII	GRGCYC	1 cut(s)	1839
BauI	CACGAG	1 cut(s)	1629
BbsI	GAAGAC	1 cut(s)	156
Bbv12I	GWGCWC	2 cut(s)	952, 1839
BbvCI	CCTCAGC	1 cut(s)	84
BbvI	GCAGC	4 cut(s)	454, 1465, 1541, 1612
BccI	CCATC	4 cut(s)	680, 1558, 1853, 2023
BceAI	ACGGC	3 cut(s)	148, 226, 1393
BcgI	CGANNNNNNTGC	2 cut(s)	1685, 1719
BciT130I	CCWGG	5 cut(s)	293, 347, 1104, 1431, 1553
BclI	TGATCA	1 cut(s)	1054
BcoDI	GTCTC	3 cut(s)	213, 2079, 2199
BcuI	ACTAGT	1 cut(s)	2030
BfaI	CTAG	6 cut(s)	1163, 1299, 1560, 2031, 2319, 2331
BfmI	CTRYAG	3 cut(s)	933, 1221, 1479
BfoI	RGCGCY	1 cut(s)	2350
BfrI	CTTAAG	1 cut(s)	1070
BfuAI	ACCTGC	1 cut(s)	2029
BglI	GCCNNNNNGGC	1 cut(s)	1246
BglII	AGATCT	1 cut(s)	2216
Bme1390I	CCNGG	5 cut(s)	293, 347, 1104, 1431, 1553
Bme18I	GGWCC	3 cut(s)	929, 1549, 1843
BmeRI	GACNNNNNGTC	1 cut(s)	95
BmgT120I	GGNCC	4 cut(s)	387, 929, 1549, 1843
BmiI	GGNNCC	1 cut(s)	853
BmrFI	CCNGG	5 cut(s)	293, 347, 1104, 1431, 1553
BmsI	GCATC	6 cut(s)	259, 472, 1023, 1070, 1134, 1602
BpiI	GAAGAC	1 cut(s)	156
BplI	GAGNNNNNCTC	2 cut(s)	292, 324
BpmI	CTGGAG	4 cut(s)	392, 1806, 2226, 2304
Bpu10I	CCTNAGC	1 cut(s)	84
Bpu14I	TTCGAA	1 cut(s)	24
BpuEI	CTTGAG	2 cut(s)	403, 1353
Bsa29I	ATCGAT	1 cut(s)	171
BsaAI	YACGTR	2 cut(s)	1151, 1280
BsaHI	GRCGYC	2 cut(s)	225, 399
BsaJI	CCNNGG	9 cut(s)	324, 471, 507, 594, 698, 1103, 1195, 1409, 1830
BsaWI	WCCGGW	3 cut(s)	657, 854, 1996
BsaXI	ACNNNNNCTCC	8 cut(s)	287, 317, 1779, 1809, 1909, 1939, 2224, 2254
Bsc4I	CCNNNNNNNGG	4 cut(s)	657, 898, 2123, 2160
Bse118I	RCCGGY	6 cut(s)	42, 227, 434, 1245, 1727, 2186
Bse1I	ACTGG	3 cut(s)	853, 969, 2255
Bse3DI	GCAATG	1 cut(s)	1857
BseAI	TCCGGA	2 cut(s)	854, 1996
BseBI	CCWGG	5 cut(s)	293, 347, 1104, 1431, 1553
BseCI	ATCGAT	1 cut(s)	171
BseDI	CCNNGG	9 cut(s)	324, 471, 507, 594, 698, 1103, 1195, 1409, 1830
BseLI	CCNNNNNNNGG	4 cut(s)	657, 898, 2123, 2160
BseMI	GCAATG	1 cut(s)	1857
BseMII	CTCAG	5 cut(s)	98, 1389, 1523, 2234, 2288
BseNI	ACTGG	3 cut(s)	853, 969, 2255
BseRI	GAGGAG	7 cut(s)	100, 103, 106, 109, 231, 374, 467
BseSI	GKGCMC	1 cut(s)	952
BseXI	GCAGC	4 cut(s)	454, 1465, 1541, 1612
BsgI	GTGCAG	1 cut(s)	2235
Bsh1236I	CGCG	3 cut(s)	126, 425, 1962
BshVI	ATCGAT	1 cut(s)	171
BsiHKAI	GWGCWC	2 cut(s)	952, 1839
BslFI	GGGAC	2 cut(s)	83, 1756
BslI	CCNNNNNNNGG	4 cut(s)	657, 898, 2123, 2160
BsmAI	GTCTC	3 cut(s)	213, 2079, 2199
BsmBI	CGTCTC	1 cut(s)	213
BsmFI	GGGAC	2 cut(s)	83, 1756
BsmI	GAATGC	1 cut(s)	754
Bsp119I	TTCGAA	1 cut(s)	24
Bsp1286I	GDGCHC	2 cut(s)	952, 1839
Bsp13I	TCCGGA	2 cut(s)	854, 1996
BspCNI	CTCAG	5 cut(s)	97, 1390, 1522, 2233, 2287
BspDI	ATCGAT	1 cut(s)	171
BspEI	TCCGGA	2 cut(s)	854, 1996
BspFNI	CGCG	3 cut(s)	126, 425, 1962
BspHI	TCATGA	1 cut(s)	744
BspLI	GGNNCC	1 cut(s)	853
BspMAI	CTGCAG	1 cut(s)	1483
BspMI	ACCTGC	1 cut(s)	2029
BspPI	GGATC	4 cut(s)	254, 372, 570, 909
BspQI	GCTCTTC	1 cut(s)	656
BspT104I	TTCGAA	1 cut(s)	24
BspTI	CTTAAG	1 cut(s)	1070
BsrDI	GCAATG	1 cut(s)	1857
BsrFI	RCCGGY	6 cut(s)	42, 227, 434, 1245, 1727, 2186
BsrI	ACTGG	3 cut(s)	853, 969, 2255
BssAI	RCCGGY	6 cut(s)	42, 227, 434, 1245, 1727, 2186
BssECI	CCNNGG	9 cut(s)	324, 471, 507, 594, 698, 1103, 1195, 1409, 1830
BssNI	GRCGYC	2 cut(s)	225, 399
BssSI	CACGAG	1 cut(s)	1629
BssT1I	CCWWGG	5 cut(s)	324, 471, 594, 698, 1195
Bst2BI	CACGAG	1 cut(s)	1629
Bst2UI	CCWGG	5 cut(s)	293, 347, 1104, 1431, 1553
Bst4CI	ACNGT	9 cut(s)	206, 725, 792, 1333, 1448, 1779, 2138, 2236, 2363
Bst6I	CTCTTC	2 cut(s)	2, 656
BstACI	GRCGYC	2 cut(s)	225, 399
BstAFI	CTTAAG	1 cut(s)	1070
BstAPI	GCANNNNNTGC	1 cut(s)	1781
BstBAI	YACGTR	2 cut(s)	1151, 1280
BstBI	TTCGAA	1 cut(s)	24
BstC8I	GCNNGC	5 cut(s)	229, 436, 1296, 2151, 2166
BstDEI	CTNAG	8 cut(s)	84, 982, 1398, 1509, 1804, 2057, 2220, 2274
BstDSI	CCRYGG	2 cut(s)	1409, 1830
BstFNI	CGCG	3 cut(s)	126, 425, 1962
BstH2I	RGCGCY	1 cut(s)	2350
BstHHI	GCGC	5 cut(s)	128, 427, 1962, 2149, 2349
BstMAI	GTCTC	3 cut(s)	213, 2079, 2199
BstNI	CCWGG	5 cut(s)	293, 347, 1104, 1431, 1553
BstNSI	RCATGY	3 cut(s)	1696, 1700, 2153
BstSCI	CCNGG	5 cut(s)	291, 345, 1102, 1429, 1551
BstSFI	CTRYAG	3 cut(s)	933, 1221, 1479
BstSLI	GKGCMC	1 cut(s)	952
BstUI	CGCG	3 cut(s)	126, 425, 1962
BstV1I	GCAGC	4 cut(s)	454, 1465, 1541, 1612
BstV2I	GAAGAC	1 cut(s)	156
BstX2I	RGATCY	1 cut(s)	2216
BstXI	CCANNNNNNTGG	2 cut(s)	478, 964
BstYI	RGATCY	1 cut(s)	2216
Bsu15I	ATCGAT	1 cut(s)	171
BsuTUI	ATCGAT	1 cut(s)	171
BtgI	CCRYGG	2 cut(s)	1409, 1830
BtsI	GCAGTG	2 cut(s)	1488, 1951
BtsIMutI	CAGTG	6 cut(s)	797, 1136, 1338, 1488, 1951, 2262
BveI	ACCTGC	1 cut(s)	2029
Cac8I	GCNNGC	5 cut(s)	229, 436, 1296, 2151, 2166
CaiI	CAGNNNCTG	1 cut(s)	2249
CciI	TCATGA	1 cut(s)	744
CfoI	GCGC	5 cut(s)	128, 427, 1962, 2149, 2349
Cfr10I	RCCGGY	6 cut(s)	42, 227, 434, 1245, 1727, 2186
Cfr13I	GGNCC	4 cut(s)	387, 929, 1549, 1843
ClaI	ATCGAT	1 cut(s)	171
CseI	GACGC	3 cut(s)	233, 407, 431
CsiI	ACCWGGT	1 cut(s)	1429
Csp6I	GTAC	6 cut(s)	343, 655, 695, 769, 875, 1382
CviQI	GTAC	6 cut(s)	343, 655, 695, 769, 875, 1382
DdeI	CTNAG	8 cut(s)	84, 982, 1398, 1509, 1804, 2057, 2220, 2274
DraI	TTTAAA	1 cut(s)	1522
DrdI	GACNNNNNNGTC	2 cut(s)	254, 455
DriI	GACNNNNNGTC	1 cut(s)	95
DseDI	GACNNNNNNGTC	2 cut(s)	254, 455
EaeI	YGGCCR	3 cut(s)	133, 669, 1350
Eam1104I	CTCTTC	2 cut(s)	2, 656
Eam1105I	GACNNNNNGTC	1 cut(s)	95
EarI	CTCTTC	2 cut(s)	2, 656
EciI	GGCGGA	3 cut(s)	228, 300, 2356
Ecl136II	GAGCTC	1 cut(s)	1837
Eco130I	CCWWGG	5 cut(s)	324, 471, 594, 698, 1195
Eco147I	AGGCCT	1 cut(s)	2164
Eco24I	GRGCYC	1 cut(s)	1839
Eco32I	GATATC	2 cut(s)	829, 1363
Eco47I	GGWCC	3 cut(s)	929, 1549, 1843
Eco53kI	GAGCTC	1 cut(s)	1837
Eco57I	CTGAAG	2 cut(s)	899, 1413
EcoICRI	GAGCTC	1 cut(s)	1837
EcoO109I	RGGNCCY	1 cut(s)	929
EcoRI	GAATTC	1 cut(s)	2278
EcoRII	CCWGG	5 cut(s)	291, 345, 1102, 1429, 1551
EcoRV	GATATC	2 cut(s)	829, 1363
EcoT14I	CCWWGG	5 cut(s)	324, 471, 594, 698, 1195
EcoT22I	ATGCAT	2 cut(s)	1698, 1767
EcoT38I	GRGCYC	1 cut(s)	1839
ErhI	CCWWGG	5 cut(s)	324, 471, 594, 698, 1195
Esp3I	CGTCTC	1 cut(s)	213
FaqI	GGGAC	2 cut(s)	83, 1756
FauI	CCCGC	2 cut(s)	131, 1955
FauNDI	CATATG	1 cut(s)	944
FbaI	TGATCA	1 cut(s)	1054
FriOI	GRGCYC	1 cut(s)	1839
FspAI	RTGCGCAY	1 cut(s)	2148
FspBI	CTAG	6 cut(s)	1163, 1299, 1560, 2031, 2319, 2331
FspI	TGCGCA	1 cut(s)	2148
GlaI	GCGC	5 cut(s)	127, 426, 1961, 2148, 2348
GsuI	CTGGAG	4 cut(s)	392, 1806, 2226, 2304
HaeII	RGCGCY	1 cut(s)	2350
HgaI	GACGC	3 cut(s)	233, 407, 431
HhaI	GCGC	5 cut(s)	128, 427, 1962, 2149, 2349
Hin1I	GRCGYC	2 cut(s)	225, 399
Hin6I	GCGC	5 cut(s)	126, 425, 1960, 2147, 2347
HinP1I	GCGC	5 cut(s)	126, 425, 1960, 2147, 2347
HindIII	AAGCTT	2 cut(s)	798, 1049
HphI	GGTGA	9 cut(s)	257, 281, 310, 583, 1349, 1460, 1553, 1742, 2010
Hpy166II	GTNNAC	3 cut(s)	529, 950, 1666
Hpy8I	GTNNAC	3 cut(s)	529, 950, 1666
Hpy99I	CGWCG	5 cut(s)	182, 227, 425, 452, 2311
HpyAV	CCTTC	9 cut(s)	20, 114, 325, 388, 920, 1126, 1398, 1849, 1900
HpyCH4III	ACNGT	9 cut(s)	206, 725, 792, 1333, 1448, 1779, 2138, 2236, 2363
HpyCH4IV	ACGT	4 cut(s)	96, 1150, 1279, 1879
HpyF3I	CTNAG	8 cut(s)	84, 982, 1398, 1509, 1804, 2057, 2220, 2274
HpySE526I	ACGT	4 cut(s)	96, 1150, 1279, 1879
Hsp92I	GRCGYC	2 cut(s)	225, 399
HspAI	GCGC	5 cut(s)	126, 425, 1960, 2147, 2347
Kpn2I	TCCGGA	2 cut(s)	854, 1996
KroI	GCCGGC	2 cut(s)	227, 434
KroNI	GCCGGC	2 cut(s)	229, 436
Ksp22I	TGATCA	1 cut(s)	1054
LguI	GCTCTTC	1 cut(s)	656
LmnI	GCTCC	5 cut(s)	313, 432, 857, 1834, 2251
Lsp1109I	GCAGC	4 cut(s)	454, 1465, 1541, 1612
LweI	GCATC	6 cut(s)	259, 472, 1023, 1070, 1134, 1602
MabI	ACCWGGT	1 cut(s)	1429
MaeI	CTAG	6 cut(s)	1163, 1299, 1560, 2031, 2319, 2331
MaeII	ACGT	4 cut(s)	96, 1150, 1279, 1879
MaeIII	GTNAC	7 cut(s)	206, 245, 1153, 1327, 2026, 2047, 2132
MboII	GAAGA	8 cut(s)	19, 161, 263, 550, 673, 935, 1875, 2262
MflI	RGATCY	1 cut(s)	2216
MhlI	GDGCHC	2 cut(s)	952, 1839
MlsI	TGGCCA	2 cut(s)	671, 1352
MluNI	TGGCCA	2 cut(s)	671, 1352
MlyI	GAGTC	4 cut(s)	84, 403, 626, 948
MmeI	TCCRAC	3 cut(s)	444, 471, 474
Mox20I	TGGCCA	2 cut(s)	671, 1352
Mph1103I	ATGCAT	2 cut(s)	1698, 1767
MreI	CGCCGGCG	1 cut(s)	227
MroI	TCCGGA	2 cut(s)	854, 1996
MroNI	GCCGGC	2 cut(s)	227, 434
MscI	TGGCCA	2 cut(s)	671, 1352
MseI	TTAA	8 cut(s)	708, 756, 1071, 1125, 1521, 1638, 1653, 1797
MslI	CAYNNNNRTG	2 cut(s)	677, 1691
Msp20I	TGGCCA	2 cut(s)	671, 1352
MspA1I	CMGCKG	1 cut(s)	467
MspCI	CTTAAG	1 cut(s)	1070
MspR9I	CCNGG	5 cut(s)	293, 347, 1104, 1431, 1553
Mva1269I	GAATGC	1 cut(s)	754
MvaI	CCWGG	5 cut(s)	293, 347, 1104, 1431, 1553
MvnI	CGCG	3 cut(s)	126, 425, 1962
NaeI	GCCGGC	2 cut(s)	229, 436
NdeI	CATATG	1 cut(s)	944
NgoMIV	GCCGGC	2 cut(s)	227, 434
NlaIV	GGNNCC	1 cut(s)	853
NmeAIII	GCCGAG	1 cut(s)	676
NmuCI	GTSAC	1 cut(s)	245
NsbI	TGCGCA	1 cut(s)	2148
NsiI	ATGCAT	2 cut(s)	1698, 1767
NspI	RCATGY	3 cut(s)	1696, 1700, 2153
NspV	TTCGAA	1 cut(s)	24
PaeI	GCATGC	1 cut(s)	2153
PagI	TCATGA	1 cut(s)	744
PaqCI	CACCTGC	1 cut(s)	2029
PceI	AGGCCT	1 cut(s)	2164
PciSI	GCTCTTC	1 cut(s)	656
PcsI	WCGNNNNNNNCGW	5 cut(s)	144, 153, 177, 255, 264
PctI	GAATGC	1 cut(s)	754
PdiI	GCCGGC	2 cut(s)	229, 436
PfeI	GAWTC	7 cut(s)	21, 286, 443, 503, 1866, 1883, 2000
PflMI	CCANNNNNTGG	1 cut(s)	898
PleI	GAGTC	4 cut(s)	84, 403, 625, 947
PpsI	GAGTC	4 cut(s)	84, 403, 625, 947
Ppu21I	YACGTR	2 cut(s)	1151, 1280
PpuMI	RGGWCCY	1 cut(s)	929
Psp124BI	GAGCTC	1 cut(s)	1839
Psp5II	RGGWCCY	1 cut(s)	929
Psp6I	CCWGG	5 cut(s)	291, 345, 1102, 1429, 1551
PspGI	CCWGG	5 cut(s)	291, 345, 1102, 1429, 1551
PspN4I	GGNNCC	1 cut(s)	853
PspPI	GGNCC	4 cut(s)	387, 929, 1549, 1843
PspPPI	RGGWCCY	1 cut(s)	929
PstI	CTGCAG	1 cut(s)	1483
PstNI	CAGNNNCTG	1 cut(s)	2249
PsuI	RGATCY	1 cut(s)	2216
RsaI	GTAC	6 cut(s)	344, 656, 696, 770, 876, 1383
RsaNI	GTAC	6 cut(s)	343, 655, 695, 769, 875, 1382
RseI	CAYNNNNRTG	2 cut(s)	677, 1691
SacI	GAGCTC	1 cut(s)	1839
SapI	GCTCTTC	1 cut(s)	656
SaqAI	TTAA	8 cut(s)	708, 756, 1071, 1125, 1521, 1638, 1653, 1797
Sau96I	GGNCC	4 cut(s)	387, 929, 1549, 1843
SchI	GAGTC	4 cut(s)	84, 403, 626, 948
ScrFI	CCNGG	5 cut(s)	293, 347, 1104, 1431, 1553
SduI	GDGCHC	2 cut(s)	952, 1839
SexAI	ACCWGGT	1 cut(s)	1429
SfaNI	GCATC	6 cut(s)	259, 472, 1023, 1070, 1134, 1602
SfcI	CTRYAG	3 cut(s)	933, 1221, 1479
SfuI	TTCGAA	1 cut(s)	24
SgrAI	CRCCGGYG	1 cut(s)	227
SinI	GGWCC	3 cut(s)	929, 1549, 1843
SmiMI	CAYNNNNRTG	2 cut(s)	677, 1691
SmlI	CTYRAG	3 cut(s)	382, 1070, 1368
SmoI	CTYRAG	3 cut(s)	382, 1070, 1368
SpeI	ACTAGT	1 cut(s)	2030
SphI	GCATGC	1 cut(s)	2153
SseBI	AGGCCT	1 cut(s)	2164
SspMI	CTAG	6 cut(s)	1163, 1299, 1560, 2031, 2319, 2331
SstI	GAGCTC	1 cut(s)	1839
StuI	AGGCCT	1 cut(s)	2164
StyD4I	CCNGG	5 cut(s)	291, 345, 1102, 1429, 1551
StyI	CCWWGG	5 cut(s)	324, 471, 594, 698, 1195
TaaI	ACNGT	9 cut(s)	206, 725, 792, 1333, 1448, 1779, 2138, 2236, 2363
TaiI	ACGT	4 cut(s)	99, 1153, 1282, 1882
TatI	WGTACW	2 cut(s)	874, 1381
TauI	GCSGC	9 cut(s)	234, 237, 240, 243, 316, 467, 869, 1944, 2045
TfiI	GAWTC	7 cut(s)	21, 286, 443, 503, 1866, 1883, 2000
Tru1I	TTAA	8 cut(s)	708, 756, 1071, 1125, 1521, 1638, 1653, 1797
Tru9I	TTAA	8 cut(s)	708, 756, 1071, 1125, 1521, 1638, 1653, 1797
TscAI	CASTG	6 cut(s)	797, 1143, 1338, 1488, 1951, 2262
TseFI	GTSAC	1 cut(s)	245
TseI	GCWGC	4 cut(s)	467, 1478, 1529, 1625
Tsp45I	GTSAC	1 cut(s)	245
TspDTI	ATGAA	8 cut(s)	265, 761, 1145, 1367, 1526, 1691, 1868, 2094
TspGWI	ACGGA	2 cut(s)	1007, 2324
TspRI	CASTG	6 cut(s)	797, 1143, 1338, 1488, 1951, 2262
Van91I	CCANNNNNTGG	1 cut(s)	898
Vha464I	CTTAAG	1 cut(s)	1070
VneI	GTGCAC	1 cut(s)	948
VpaK11BI	GGWCC	3 cut(s)	929, 1549, 1843
XapI	RAATTY	4 cut(s)	995, 1305, 2278, 2290
XbaI	TCTAGA	1 cut(s)	2318
XceI	RCATGY	3 cut(s)	1696, 1700, 2153
XcmI	CCANNNNNNNNNTGG	1 cut(s)	409
XspI	CTAG	6 cut(s)	1163, 1299, 1560, 2031, 2319, 2331
Zsp2I	ATGCAT	2 cut(s)	1698, 1767

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.