Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0003007

Physical Location & Seq

Genomic Coordinates Forward (+)

33697912 .. 33701815

Gene Structure 3904 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1276500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1971 bp

ATGGAGAGTTTTCTGAGGGCTAAAAAGGACTTGGTGCTCACGCTGGCATCGTATGCTTTACACGCCGGATATGACTCCGATGAGGAGGTATATGCGGCCGCCAAGGCCGTCGATGCCGGGTTGTTGGAGTACGATTCAGATGATAATCCCATTGTTCTGGACAAGAGGAAGATCGAGCCGATTCCAGCTCTCGATCACAGTTCAATCGACTACGAGCCGTTTAATAAGGATTTTTACGAGGAGAAAGAGTCGATTTCAGTCGAAATGCGTAGCAAAGTATTCAAAGGCTTGGCCATCCGTACGTCGGGTTTTGATGTACCGAGGCCAGTTAAGACATTTGAAGATTGTGGATTTTCTTCACAACTCATGGCTGCCATAAAAAAACAAGACTACCAAAAGCCGACACCAATACAATGCGCAGCTCTACCCATAGTTCTTTCCGGGAGAGATATAATTGGTATTGCGAAAACTGGTTCTGGAAAGACTGCCGCTTTTGTCCTTCCAATGATTGTCCACATTATGGATCAGCCAGAACTTCAAAAAGAAGAAGGTCCCATTGGAGTGATATGCGCTCCTACTAGAGAGCTGGCGCACCAAATATACTTAGAGTCCAAAAAATTTGCGAAATCACATGGGATACGTGTCTCTGCTGTATATGGTGGAATGTCTAAGCTTGATCAGTTCAAAGAACTTAAGGCAGGATGTGACATAGTTGTTGCTACTCCTGGGAGATTGATAGACATGCTTAAAATGAAGGCACTGACAATGCAAAGGGCAACTTACCTAGTACTTGATGAGGCTGATCGGATGTTTGACCTAGGGTTTGAGCCTCAAATTAGATCTATTGTTGGTCAGATTCGGCCAGACCGACAGACATTGCTCTTTTATGCGACAATGCCCCGTAAAGTTGAAAAATTGGCTAGGGAGATTCTCTCAGATCCTATAAGAGTTACAGTGGGTGAGGTGGGAATGGCCAATGAGGATATCACTCAGGTTGTTCATGTAATTCCTTCTGATGATGAAAAGCTGCCCTGGCTTCTTGAGAAGTTACCTGGAATGATTGATGAGGGTGATGTTTTAGTATTTGCTTCAAAAAAAGTTGCAGTGGATGAGATCGAGTCACAGCTTGCTCAGAAAGGTTTTAAAGTCACAGCTCTGCATGGTGACAAGGACCAGGCATCTCGGATGGATATTTTGCAAAAATTTAAATCTGGCATTTACCATGTTCTTGTTGCAACTGATGTTGCTGCTCGTGGTCTTGACATCAAGTCAATTAAGTCAGTTGTGAACTTTGATATTGCAAAGGACATGGACATGCACGTCCATCGAATTGGTAGAACAGGTCGGGCTGGTGATAAGGATGGCACTGCGTACGCTCTTATTGCACAGAAAGAGGCACGTTTTGCTGGTGAGTTGGTTAATAGCTTGGTTGCTGCTGGTCAGATTGTCTCCACGGAGCTCATGGACCTTGCGATGAAGGATGGTAGATTCAGGTCAAAACGCGATTCAAGAAAAGGAGGTGGAAAGAAAGGTAGAGGAAGGGGCGGCGGTGGTCGAGGTGTGCGTGAGGTGGATTTTGGTCTGGGTATTGGATATAATACGGAGTCCAATAGTTCTTCATCGAATACTGTTCCTAGTCGATCTGCCGCAGAAACTCCTCAGAGGACAGGAATGATGTCTCAGTTCAAGACTAAATTTGTTGCCGCTTCATCTAACTCTCCAAGTCAAGGTTCAGGTAACAACTCTAGTGTGCCCAACAGACCGGCATTACGAGGATTTGTATCCGATGGTTCAATCGGTGGGGATGTATATAGAACTCAGACAGCCAGTACAATTACTCCTGGTCCTCCTACATCAGTGGTAAATAAAACTGCTGCTCGGAACACCGGAGCAAATACAAGTCAGAAGGCCTCCGAAAGTTCTAGAGACAAACCTAGAGAAAGGCGGCGGCGCTCCGGTTGGGACTGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0006139 GO:0006725 GO:0006807 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0009987 GO:0010501 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0031974 GO:0031981 GO:0034641 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0070013 GO:0070035 GO:0071704 GO:0090304 GO:0140098 GO:1901360

KEGG Pathways

Metabolic & Signaling

ko03040 map03040

Pfam Domains

Protein Families

DEAD Helicase_C

Protein Analysis

657

Amino Acids

71.56

Weight (kDa)

8.97

Isoelectric Point (pI)

38.63

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DEAD	PF00270	135 - 305	2.4e-49	DEAD/DEAH box helicase
Helicase_C	PF00271	352 - 451	1.1e-26	Helicase conserved C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0003007)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G47330
fragaria_vesca	FvH4_1g27100 FvH4_1g27100 FvH4_1g27100
malus_domestica	MD00G1118300.v1.1 MD00G1118400.v1.1 MD09G1283400.v1.1 MD17G1274100.v1.1 MD17G1274800.v1.1 MD17G1275300.v1.1 MD17G1276500.v1.1
prunus_persica	Prupe.3G010900_v2.0.a1 Prupe.3G010900_v2.0.a1 Prupe.3G010900_v2.0.a1 Prupe.3G010900_v2.0.a1 Prupe.3G010900_v2.0.a1
pyrus_communis	pycom09g19130 pycom09g19140 pycom17g27210 pycom17g27330
rosa_chinensis	RchiOBHm_Chr3g0487421
rosa_laevigata	RLG00000023004
rosa_multiflora	Rmu_sc0008232.1_g000009 Rmu_sc0022533.1_g000001 Rmu_sc0031316.1_g000001
rosa_roxburghii	Rroxscaffold_6G00394790
rosa_rugosa	Rorug03G0234000
rosa_samantha	Rh2AG395100 Rh2CG381700 Rh3AG282000 Rh3BG317600 Rh3CG315700 Rh3DG313600 Rh4CG235900 Rh7AG337700 Rh7AG476200 Rh7CG355800
rosa_wichuraiana	Rw3G024970

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1319
Acc16I	TGCGCA	1 cut(s)	418
AccB7I	CCANNNNNTGG	1 cut(s)	520
AccII	CGCG	1 cut(s)	1503
AciI	CCGC	9 cut(s)	95, 99, 489, 1545, 1548, 1647, 1704, 1945, 1948
AclWI	GGATC	2 cut(s)	531, 932
AcoI	YGGCCR	4 cut(s)	96, 291, 860, 972
AcsI	RAATTY	3 cut(s)	617, 1202, 1694
AfaI	GTAC	6 cut(s)	131, 301, 318, 789, 1373, 1831
AfiI	CCNNNNNNNGG	3 cut(s)	304, 520, 1727
AflII	CTTAAG	1 cut(s)	692
AflIII	ACRYGT	1 cut(s)	640
AhdI	GACNNNNNGTC	1 cut(s)	1267
AjiI	CACGTC	1 cut(s)	1321
AjnI	CCWGG	5 cut(s)	724, 1031, 1051, 1173, 1840
AjuI	GAANNNNNNNTTGG	4 cut(s)	540, 572, 1714, 1746
AluBI	AGCT	9 cut(s)	188, 422, 586, 673, 1027, 1126, 1154, 1425, 1459
AluI	AGCT	9 cut(s)	188, 422, 586, 673, 1027, 1126, 1154, 1425, 1459
Alw21I	GWGCWC	2 cut(s)	39, 1461
Alw26I	GTCTC	4 cut(s)	649, 1453, 1683, 1920
AlwI	GGATC	2 cut(s)	531, 932
AoxI	GGCC	7 cut(s)	96, 105, 291, 323, 860, 972, 1908
ApeKI	GCWGC	6 cut(s)	371, 419, 1027, 1247, 1433, 1874
ApoI	RAATTY	3 cut(s)	617, 1202, 1694
ArsI	GACNNNNNNTTYG	2 cut(s)	806, 838
AspA2I	CCTAGG	1 cut(s)	817
AspLEI	GCGC	4 cut(s)	419, 572, 592, 1953
AspS9I	GGNCC	4 cut(s)	551, 1171, 1465, 1844
AsuC2I	CCSGG	2 cut(s)	118, 442
AsuHPI	GGTGA	5 cut(s)	971, 1082, 1175, 1364, 1421
AvaII	GGWCC	4 cut(s)	551, 1171, 1465, 1844
AvrII	CCTAGG	1 cut(s)	817
BaeGI	GKGCMC	1 cut(s)	1755
BalI	TGGCCA	2 cut(s)	293, 974
BanII	GRGCYC	1 cut(s)	1461
BauI	CACGAG	1 cut(s)	1251
Bbv12I	GWGCWC	2 cut(s)	39, 1461
BbvI	GCAGC	6 cut(s)	358, 431, 1014, 1234, 1420, 1861
BccI	CCATC	6 cut(s)	302, 1180, 1332, 1355, 1475, 1781
BceAI	ACGGC	2 cut(s)	92, 202
BcgI	CGANNNNNNTGC	2 cut(s)	1307, 1341
BciT130I	CCWGG	5 cut(s)	726, 1033, 1053, 1175, 1842
BciVI	GTATCC	2 cut(s)	630, 1792
BclI	TGATCA	1 cut(s)	676
BcnI	CCSGG	2 cut(s)	118, 442
BcoDI	GTCTC	4 cut(s)	649, 1453, 1683, 1920
BfaI	CTAG	8 cut(s)	579, 785, 818, 921, 1635, 1746, 1923, 1935
BfoI	RGCGCY	1 cut(s)	1954
BfrI	CTTAAG	1 cut(s)	692
BfuI	GTATCC	2 cut(s)	630, 1792
BglI	GCCNNNNNGGC	1 cut(s)	104
BglII	AGATCT	1 cut(s)	839
BlnI	CCTAGG	1 cut(s)	817
BmcAI	AGTACT	1 cut(s)	789
Bme1390I	CCNGG	7 cut(s)	118, 442, 726, 1033, 1053, 1175, 1842
Bme18I	GGWCC	4 cut(s)	551, 1171, 1465, 1844
BmeRI	GACNNNNNGTC	1 cut(s)	1267
BmgBI	CACGTC	1 cut(s)	1321
BmgT120I	GGNCC	4 cut(s)	551, 1171, 1465, 1844
BmiI	GGNNCC	1 cut(s)	553
BmrFI	CCNGG	7 cut(s)	118, 442, 726, 1033, 1053, 1175, 1842
BmsI	GCATC	3 cut(s)	56, 103, 1187
BplI	GAGNNNNNCTC	2 cut(s)	917, 949
BpuEI	CTTGAG	1 cut(s)	1061
BpuMI	CCSGG	2 cut(s)	118, 442
BsaAI	YACGTR	1 cut(s)	641
BsaBI	GATNNNNATC	2 cut(s)	144, 1780
BsaJI	CCNNGG	6 cut(s)	102, 320, 725, 817, 1031, 1452
BsaWI	WCCGGW	2 cut(s)	1886, 1955
BsaXI	ACNNNNNCTCC	4 cut(s)	233, 263, 1822, 1852
Bsc4I	CCNNNNNNNGG	3 cut(s)	304, 520, 1727
Bse118I	RCCGGY	1 cut(s)	1762
Bse1I	ACTGG	3 cut(s)	326, 475, 1827
Bse3DI	GCAATG	1 cut(s)	875
Bse8I	GATNNNNATC	2 cut(s)	144, 1780
BseBI	CCWGG	5 cut(s)	726, 1033, 1053, 1175, 1842
BseDI	CCNNGG	6 cut(s)	102, 320, 725, 817, 1031, 1452
BseGI	GGATG	8 cut(s)	294, 707, 813, 1114, 1191, 1366, 1486, 1810
BseJI	GATNNNNATC	2 cut(s)	144, 1780
BseLI	CCNNNNNNNGG	3 cut(s)	304, 520, 1727
BseMI	GCAATG	1 cut(s)	875
BseMII	CTCAG	7 cut(s)	5, 948, 1004, 1145, 1673, 1694, 1832
BseNI	ACTGG	3 cut(s)	326, 475, 1827
BseRI	GAGGAG	3 cut(s)	98, 254, 1647
BseSI	GKGCMC	1 cut(s)	1755
BseX3I	CGGCCG	1 cut(s)	96
BseXI	GCAGC	6 cut(s)	358, 431, 1014, 1234, 1420, 1861
Bsh1236I	CGCG	1 cut(s)	1503
Bsh1285I	CGRYCG	1 cut(s)	99
BshFI	GGCC	7 cut(s)	98, 107, 293, 325, 862, 974, 1910
BsiEI	CGRYCG	1 cut(s)	99
BsiHKAI	GWGCWC	2 cut(s)	39, 1461
BsiSI	CCGG	6 cut(s)	66, 117, 441, 1763, 1887, 1956
BsiWI	CGTACG	2 cut(s)	299, 1371
BslFI	GGGAC	1 cut(s)	537
BslI	CCNNNNNNNGG	3 cut(s)	304, 520, 1727
BsmAI	GTCTC	4 cut(s)	649, 1453, 1683, 1920
BsmFI	GGGAC	1 cut(s)	537
BsnI	GGCC	7 cut(s)	98, 107, 293, 325, 862, 974, 1910
Bsp1286I	GDGCHC	3 cut(s)	39, 1461, 1755
Bsp143I	GATC	9 cut(s)	171, 193, 523, 676, 802, 839, 937, 1113, 1640
BspACI	CCGC	9 cut(s)	95, 99, 489, 1545, 1548, 1647, 1704, 1945, 1948
BspANI	GGCC	7 cut(s)	98, 107, 293, 325, 862, 974, 1910
BspCNI	CTCAG	7 cut(s)	6, 947, 1003, 1144, 1672, 1693, 1831
BspFNI	CGCG	1 cut(s)	1503
BspLI	GGNNCC	1 cut(s)	553
BspPI	GGATC	2 cut(s)	531, 932
BspTI	CTTAAG	1 cut(s)	692
BsrDI	GCAATG	1 cut(s)	875
BsrFI	RCCGGY	1 cut(s)	1762
BsrI	ACTGG	3 cut(s)	326, 475, 1827
BssAI	RCCGGY	1 cut(s)	1762
BssECI	CCNNGG	6 cut(s)	102, 320, 725, 817, 1031, 1452
BssMI	GATC	9 cut(s)	171, 193, 523, 676, 802, 839, 937, 1113, 1640
BssSI	CACGAG	1 cut(s)	1251
BssT1I	CCWWGG	2 cut(s)	102, 817
Bst2BI	CACGAG	1 cut(s)	1251
Bst2UI	CCWGG	5 cut(s)	726, 1033, 1053, 1175, 1842
Bst4CI	ACNGT	4 cut(s)	200, 955, 1630, 1967
BstAFI	CTTAAG	1 cut(s)	692
BstAPI	GCANNNNNTGC	2 cut(s)	53, 1403
BstBAI	YACGTR	1 cut(s)	641
BstC8I	GCNNGC	3 cut(s)	45, 588, 1128
BstDEI	CTNAG	9 cut(s)	14, 604, 669, 934, 990, 1131, 1659, 1680, 1818
BstDSI	CCRYGG	1 cut(s)	1452
BstF5I	GGATG	8 cut(s)	294, 707, 813, 1114, 1191, 1366, 1486, 1810
BstFNI	CGCG	1 cut(s)	1503
BstH2I	RGCGCY	1 cut(s)	1954
BstHHI	GCGC	4 cut(s)	419, 572, 592, 1953
BstKTI	GATC	9 cut(s)	174, 196, 526, 679, 805, 842, 940, 1116, 1643
BstMAI	GTCTC	4 cut(s)	649, 1453, 1683, 1920
BstMBI	GATC	9 cut(s)	171, 193, 523, 676, 802, 839, 937, 1113, 1640
BstMCI	CGRYCG	1 cut(s)	99
BstMWI	GCNNNNNNNGC	7 cut(s)	53, 62, 104, 113, 1033, 1382, 1403
BstNI	CCWGG	5 cut(s)	726, 1033, 1053, 1175, 1842
BstNSI	RCATGY	2 cut(s)	745, 1318
BstSCI	CCNGG	7 cut(s)	116, 440, 724, 1031, 1051, 1173, 1840
BstSLI	GKGCMC	1 cut(s)	1755
BstUI	CGCG	1 cut(s)	1503
BstV1I	GCAGC	6 cut(s)	358, 431, 1014, 1234, 1420, 1861
BstX2I	RGATCY	2 cut(s)	839, 937
BstXI	CCANNNNNNTGG	2 cut(s)	157, 1331
BstYI	RGATCY	2 cut(s)	839, 937
BstZI	CGGCCG	1 cut(s)	96
BsuI	GTATCC	2 cut(s)	630, 1792
BsuRI	GGCC	7 cut(s)	98, 107, 293, 325, 862, 974, 1910
BtgI	CCRYGG	1 cut(s)	1452
BtgZI	GCGATG	1 cut(s)	1487
BtrI	CACGTC	1 cut(s)	1321
BtsCI	GGATG	8 cut(s)	294, 707, 813, 1114, 1191, 1366, 1486, 1810
BtsI	GCAGTG	2 cut(s)	1110, 1365
BtsIMutI	CAGTG	5 cut(s)	758, 960, 1110, 1365, 1863
Cac8I	GCNNGC	3 cut(s)	45, 588, 1128
CciNI	GCGGCCGC	1 cut(s)	96
CfoI	GCGC	4 cut(s)	419, 572, 592, 1953
Cfr10I	RCCGGY	1 cut(s)	1762
Cfr13I	GGNCC	4 cut(s)	551, 1171, 1465, 1844
Csp6I	GTAC	6 cut(s)	130, 300, 317, 788, 1372, 1830
CviAII	CATG	9 cut(s)	367, 632, 742, 1001, 1160, 1223, 1309, 1315, 1462
CviQI	GTAC	6 cut(s)	130, 300, 317, 788, 1372, 1830
DdeI	CTNAG	9 cut(s)	14, 604, 669, 934, 990, 1131, 1659, 1680, 1818
DpnI	GATC	9 cut(s)	173, 195, 525, 678, 804, 841, 939, 1115, 1642
DpnII	GATC	9 cut(s)	171, 193, 523, 676, 802, 839, 937, 1113, 1640
DraI	TTTAAA	2 cut(s)	1144, 1207
DrdI	GACNNNNNNGTC	1 cut(s)	1319
DriI	GACNNNNNGTC	1 cut(s)	1267
DseDI	GACNNNNNNGTC	1 cut(s)	1319
EaeI	YGGCCR	4 cut(s)	96, 291, 860, 972
EagI	CGGCCG	1 cut(s)	96
Eam1105I	GACNNNNNGTC	1 cut(s)	1267
Ecl136II	GAGCTC	1 cut(s)	1459
EclXI	CGGCCG	1 cut(s)	96
Eco130I	CCWWGG	2 cut(s)	102, 817
Eco147I	AGGCCT	1 cut(s)	1910
Eco24I	GRGCYC	1 cut(s)	1461
Eco32I	GATATC	1 cut(s)	985
Eco47I	GGWCC	4 cut(s)	551, 1171, 1465, 1844
Eco52I	CGGCCG	1 cut(s)	96
Eco53kI	GAGCTC	1 cut(s)	1459
EcoICRI	GAGCTC	1 cut(s)	1459
EcoO109I	RGGNCCY	1 cut(s)	551
EcoRII	CCWGG	5 cut(s)	724, 1031, 1051, 1173, 1840
EcoRV	GATATC	1 cut(s)	985
EcoT14I	CCWWGG	2 cut(s)	102, 817
EcoT38I	GRGCYC	1 cut(s)	1461
ErhI	CCWWGG	2 cut(s)	102, 817
FaeI	CATG	9 cut(s)	370, 635, 745, 1004, 1163, 1226, 1312, 1318, 1465
FalI	AAGNNNNNCTT	2 cut(s)	763, 795
FaqI	GGGAC	1 cut(s)	537
FatI	CATG	9 cut(s)	366, 631, 741, 1000, 1159, 1222, 1308, 1314, 1461
FbaI	TGATCA	1 cut(s)	676
FokI	GGATG	8 cut(s)	281, 714, 820, 1121, 1198, 1373, 1493, 1817
FriOI	GRGCYC	1 cut(s)	1461
FspBI	CTAG	8 cut(s)	579, 785, 818, 921, 1635, 1746, 1923, 1935
FspI	TGCGCA	1 cut(s)	418
GlaI	GCGC	4 cut(s)	418, 571, 591, 1952
HaeII	RGCGCY	1 cut(s)	1954
HaeIII	GGCC	7 cut(s)	98, 107, 293, 325, 862, 974, 1910
HapII	CCGG	6 cut(s)	66, 117, 441, 1763, 1887, 1956
HhaI	GCGC	4 cut(s)	419, 572, 592, 1953
Hin1II	CATG	9 cut(s)	370, 635, 745, 1004, 1163, 1226, 1312, 1318, 1465
Hin6I	GCGC	4 cut(s)	417, 570, 590, 1951
HinP1I	GCGC	4 cut(s)	417, 570, 590, 1951
HindIII	AAGCTT	1 cut(s)	671
HpaII	CCGG	6 cut(s)	66, 117, 441, 1763, 1887, 1956
HphI	GGTGA	5 cut(s)	971, 1082, 1175, 1364, 1421
Hpy166II	GTNNAC	2 cut(s)	514, 1288
Hpy188III	TCNNGA	8 cut(s)	158, 191, 477, 1040, 1259, 1509, 1687, 1923
Hpy8I	GTNNAC	2 cut(s)	514, 1288
Hpy99I	CGWCG	2 cut(s)	113, 307
HpyAV	CCTTC	7 cut(s)	509, 542, 748, 1020, 1471, 1533, 1900
HpyCH4III	ACNGT	4 cut(s)	200, 955, 1630, 1967
HpyCH4IV	ACGT	4 cut(s)	302, 640, 1320, 1399
HpyCH4V	TGCA	8 cut(s)	769, 1103, 1159, 1198, 1235, 1301, 1318, 1385
HpyF10VI	GCNNNNNNNGC	7 cut(s)	53, 62, 104, 113, 1033, 1382, 1403
HpyF3I	CTNAG	9 cut(s)	14, 604, 669, 934, 990, 1131, 1659, 1680, 1818
HpySE526I	ACGT	4 cut(s)	302, 640, 1320, 1399
Hsp92II	CATG	9 cut(s)	370, 635, 745, 1004, 1163, 1226, 1312, 1318, 1465
HspAI	GCGC	4 cut(s)	417, 570, 590, 1951
Ksp22I	TGATCA	1 cut(s)	676
Kzo9I	GATC	9 cut(s)	171, 193, 523, 676, 802, 839, 937, 1113, 1640
LmnI	GCTCC	4 cut(s)	577, 1456, 1889, 1958
Lsp1109I	GCAGC	6 cut(s)	358, 431, 1014, 1234, 1420, 1861
LweI	GCATC	3 cut(s)	56, 103, 1187
MaeI	CTAG	8 cut(s)	579, 785, 818, 921, 1635, 1746, 1923, 1935
MaeII	ACGT	4 cut(s)	302, 640, 1320, 1399
MaeIII	GTNAC	7 cut(s)	704, 949, 1047, 1119, 1147, 1163, 1736
MalI	GATC	9 cut(s)	173, 195, 525, 678, 804, 841, 939, 1115, 1642
MboI	GATC	9 cut(s)	171, 193, 523, 676, 802, 839, 937, 1113, 1640
MboII	GAAGA	5 cut(s)	181, 348, 353, 557, 1608
MflI	RGATCY	2 cut(s)	839, 937
MhlI	GDGCHC	3 cut(s)	39, 1461, 1755
MlsI	TGGCCA	2 cut(s)	293, 974
MluNI	TGGCCA	2 cut(s)	293, 974
MlyI	GAGTC	5 cut(s)	68, 257, 617, 1127, 1613
MmeI	TCCRAC	1 cut(s)	105
Mox20I	TGGCCA	2 cut(s)	293, 974
MscI	TGGCCA	2 cut(s)	293, 974
MseI	TTAA	8 cut(s)	222, 330, 693, 747, 1143, 1206, 1275, 1419
MslI	CAYNNNNRTG	2 cut(s)	560, 1313
Msp20I	TGGCCA	2 cut(s)	293, 974
MspCI	CTTAAG	1 cut(s)	692
MspI	CCGG	6 cut(s)	66, 117, 441, 1763, 1887, 1956
MspR9I	CCNGG	7 cut(s)	118, 442, 726, 1033, 1053, 1175, 1842
MvaI	CCWGG	5 cut(s)	726, 1033, 1053, 1175, 1842
MvnI	CGCG	1 cut(s)	1503
MwoI	GCNNNNNNNGC	7 cut(s)	53, 62, 104, 113, 1033, 1382, 1403
NciI	CCSGG	2 cut(s)	118, 442
NdeII	GATC	9 cut(s)	171, 193, 523, 676, 802, 839, 937, 1113, 1640
NlaIII	CATG	9 cut(s)	370, 635, 745, 1004, 1163, 1226, 1312, 1318, 1465
NlaIV	GGNNCC	1 cut(s)	553
NmuCI	GTSAC	4 cut(s)	704, 1119, 1147, 1163
NotI	GCGGCCGC	1 cut(s)	96
NsbI	TGCGCA	1 cut(s)	418
NspI	RCATGY	2 cut(s)	745, 1318
PceI	AGGCCT	1 cut(s)	1910
PcsI	WCGNNNNNNNCGW	3 cut(s)	47, 865, 1561
PfeI	GAWTC	6 cut(s)	134, 181, 856, 928, 1488, 1505
Pfl23II	CGTACG	2 cut(s)	299, 1371
PflMI	CCANNNNNTGG	1 cut(s)	520
PfoI	TCCNGGA	1 cut(s)	440
PleI	GAGTC	5 cut(s)	68, 256, 616, 1126, 1612
PpsI	GAGTC	5 cut(s)	68, 256, 616, 1126, 1612
Ppu21I	YACGTR	1 cut(s)	641
PpuMI	RGGWCCY	1 cut(s)	551
Psp124BI	GAGCTC	1 cut(s)	1461
Psp5II	RGGWCCY	1 cut(s)	551
Psp6I	CCWGG	5 cut(s)	724, 1031, 1051, 1173, 1840
PspGI	CCWGG	5 cut(s)	724, 1031, 1051, 1173, 1840
PspLI	CGTACG	2 cut(s)	299, 1371
PspN4I	GGNNCC	1 cut(s)	553
PspPI	GGNCC	4 cut(s)	551, 1171, 1465, 1844
PspPPI	RGGWCCY	1 cut(s)	551
PsuI	RGATCY	2 cut(s)	839, 937
RsaI	GTAC	6 cut(s)	131, 301, 318, 789, 1373, 1831
RsaNI	GTAC	6 cut(s)	130, 300, 317, 788, 1372, 1830
RseI	CAYNNNNRTG	2 cut(s)	560, 1313
SacI	GAGCTC	1 cut(s)	1461
SaqAI	TTAA	8 cut(s)	222, 330, 693, 747, 1143, 1206, 1275, 1419
Sau3AI	GATC	9 cut(s)	171, 193, 523, 676, 802, 839, 937, 1113, 1640
Sau96I	GGNCC	4 cut(s)	551, 1171, 1465, 1844
ScaI	AGTACT	1 cut(s)	789
SchI	GAGTC	5 cut(s)	68, 257, 617, 1127, 1613
ScrFI	CCNGG	7 cut(s)	118, 442, 726, 1033, 1053, 1175, 1842
SduI	GDGCHC	3 cut(s)	39, 1461, 1755
SfaNI	GCATC	3 cut(s)	56, 103, 1187
SfiI	GGCCNNNNNGGCC	1 cut(s)	104
SinI	GGWCC	4 cut(s)	551, 1171, 1465, 1844
SmiI	ATTTAAAT	1 cut(s)	1207
SmiMI	CAYNNNNRTG	2 cut(s)	560, 1313
SmlI	CTYRAG	2 cut(s)	692, 1040
SmoI	CTYRAG	2 cut(s)	692, 1040
SseBI	AGGCCT	1 cut(s)	1910
SsiI	CCGC	9 cut(s)	95, 99, 489, 1545, 1548, 1647, 1704, 1945, 1948
SspMI	CTAG	8 cut(s)	579, 785, 818, 921, 1635, 1746, 1923, 1935
SstI	GAGCTC	1 cut(s)	1461
StuI	AGGCCT	1 cut(s)	1910
StyD4I	CCNGG	7 cut(s)	116, 440, 724, 1031, 1051, 1173, 1840
StyI	CCWWGG	2 cut(s)	102, 817
SwaI	ATTTAAAT	1 cut(s)	1207
TaaI	ACNGT	4 cut(s)	200, 955, 1630, 1967
TaiI	ACGT	4 cut(s)	305, 643, 1323, 1402
TaqII	GACCGA	1 cut(s)	882
TatI	WGTACW	2 cut(s)	787, 1829
TauI	GCSGC	8 cut(s)	98, 101, 491, 1548, 1649, 1706, 1948, 1951
TfiI	GAWTC	6 cut(s)	134, 181, 856, 928, 1488, 1505
Tru1I	TTAA	8 cut(s)	222, 330, 693, 747, 1143, 1206, 1275, 1419
Tru9I	TTAA	8 cut(s)	222, 330, 693, 747, 1143, 1206, 1275, 1419
TscAI	CASTG	5 cut(s)	765, 960, 1110, 1372, 1863
TseFI	GTSAC	4 cut(s)	704, 1119, 1147, 1163
TseI	GCWGC	6 cut(s)	371, 419, 1027, 1247, 1433, 1874
Tsp45I	GTSAC	4 cut(s)	704, 1119, 1147, 1163
TspDTI	ATGAA	6 cut(s)	767, 989, 1035, 1490, 1608, 1698
TspGWI	ACGGA	3 cut(s)	287, 1469, 1616
TspRI	CASTG	5 cut(s)	765, 960, 1110, 1372, 1863
Van91I	CCANNNNNTGG	1 cut(s)	520
Vha464I	CTTAAG	1 cut(s)	692
VpaK11BI	GGWCC	4 cut(s)	551, 1171, 1465, 1844
XapI	RAATTY	3 cut(s)	617, 1202, 1694
XbaI	TCTAGA	1 cut(s)	1922
XceI	RCATGY	2 cut(s)	745, 1318
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1459
XmaJI	CCTAGG	1 cut(s)	817
XspI	CTAG	8 cut(s)	579, 785, 818, 921, 1635, 1746, 1923, 1935
ZrmI	AGTACT	1 cut(s)	789

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.