FvH4_2g19990

Membrane-associated 30 kDa protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0002961
Physical Location & Seq
Forward (+)
16842124 .. 16846993
4870 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g19990.t1

Sequence Viewer

Length: 783 bp
ATGAACCTTTTTGACCGGTTTTCTAGAGTTGTCAAGTCATATGCAAATGCAATCATAAGTTCCTTTGAAGATCCTGAGAAAATCTTGGAGCAAGCAGTTCTTGAAATGAATGATGACTTGACAAAGATGCGTCAGGCCACAGCACAAGTATTGGCTTCTCAAAAACGTTTGGAAAATAAGTATAAAGCTGCGCAACAAGCTTCTGAGGACTGGTATCGAAAAGCACAATTAGCTCTTCAGAAAGGAGAGGAGGATCTTGCACGTGAAGCTCTGAAGAGGCGTAAATCTTTTGAGGATAATGCTAATTCTATGAGAACTCAACTTGATCAACAAAAAAGTGTTGTTGATAATCTTGTGGCTAATACAAAGCTTTTAGAGAGCAAGATACAAGAGGCAAGGTCGAAAAAAGATACCCTCAAAGCACGCGCTCAGTCTGCAAAGACTGCTACTAAAGTGAGTGAGATGGTGGGGAATGTCAATACAAGTAGTGCCCTTTCTGCTTTTGACAAGATGGAAGAGAAAGTCTTAGCAATGGAATCCCAAGCAGAAGCTCTTGGCCAGTTAACTACAGATGATTTGGAAGGAAAGTTTGCAATGCTAGAGAGCTCTTCTGTTGATGATGATCTTGCAAAGTTGAAGAAAGAACTGTCTGGTAGTTCAAAGAAAGGAGATCTTCCACCTGGAAGAACTGTTACTCCCAGTTCGAACAAGGCATATCCATTCAAGGATTCTGAAATTGAAATGGAGCTTAATGAATTGAGAAGGAAAGCACAAGACTTCTAA

Protein Analysis

261

Amino Acids

29.16

Weight (kDa)

6.53

Isoelectric Point (pI)

46.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PspA_IM30 PF04012 2 - 215 1.9e-54 PspA/IM30 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 192
AccII CGCG 1 cut(s) 426
AclI AACGTT 1 cut(s) 166
AclWI GGATC 2 cut(s) 65, 261
AcoI YGGCCR 1 cut(s) 556
AcuI CTGAAG 2 cut(s) 221, 293
AcvI CACGTG 1 cut(s) 263
AgeI ACCGGT 1 cut(s) 15
AgsI TTSAA 6 cut(s) 68, 104, 637, 660, 724, 740
AjnI CCWGG 1 cut(s) 679
AloI GAACNNNNNNTCC 2 cut(s) 679, 711
AluBI AGCT 8 cut(s) 188, 200, 233, 269, 370, 551, 606, 748
AluI AGCT 8 cut(s) 188, 200, 233, 269, 370, 551, 606, 748
Alw21I GWGCWC 1 cut(s) 608
AlwI GGATC 2 cut(s) 65, 261
AoxI GGCC 2 cut(s) 135, 556
ApeKI GCWGC 1 cut(s) 188
AsiGI ACCGGT 1 cut(s) 15
AspLEI GCGC 2 cut(s) 193, 428
AsuII TTCGAA 1 cut(s) 704
BaeGI GKGCMC 1 cut(s) 493
BalI TGGCCA 1 cut(s) 558
BanII GRGCYC 1 cut(s) 608
BarI GAAGNNNNNNTAC 2 cut(s) 676, 708
BbrPI CACGTG 1 cut(s) 263
Bbv12I GWGCWC 1 cut(s) 608
BbvI GCAGC 1 cut(s) 175
BccI CCATC 2 cut(s) 457, 505
BciT130I CCWGG 1 cut(s) 681
BclI TGATCA 1 cut(s) 325
BfaI CTAG 2 cut(s) 24, 599
BfmI CTRYAG 1 cut(s) 567
BglII AGATCT 1 cut(s) 670
BisI GCNGC 1 cut(s) 189
BlsI GCNGC 1 cut(s) 190
Bme1390I CCNGG 1 cut(s) 681
BmrFI CCNGG 1 cut(s) 681
BmrI ACTGGG 1 cut(s) 693
BmsI GCATC 1 cut(s) 117
BmuI ACTGGG 1 cut(s) 693
Bpu14I TTCGAA 1 cut(s) 704
BsaAI YACGTR 1 cut(s) 263
BsaBI GATNNNNATC 1 cut(s) 621
BsaWI WCCGGW 1 cut(s) 15
BsaXI ACNNNNNCTCC 2 cut(s) 679, 709
Bse118I RCCGGY 1 cut(s) 15
Bse1I ACTGG 3 cut(s) 215, 559, 699
Bse3DI GCAATG 2 cut(s) 537, 600
Bse8I GATNNNNATC 1 cut(s) 621
BseBI CCWGG 1 cut(s) 681
BseJI GATNNNNATC 1 cut(s) 621
BseMI GCAATG 2 cut(s) 537, 600
BseMII CTCAG 3 cut(s) 66, 195, 443
BseNI ACTGG 3 cut(s) 215, 559, 699
BseRI GAGGAG 1 cut(s) 263
BseSI GKGCMC 1 cut(s) 493
BseXI GCAGC 1 cut(s) 175
Bsh1236I CGCG 1 cut(s) 426
BshFI GGCC 2 cut(s) 137, 558
BshTI ACCGGT 1 cut(s) 15
BsiHKAI GWGCWC 1 cut(s) 608
BsiSI CCGG 1 cut(s) 16
BsnI GGCC 2 cut(s) 137, 558
Bsp119I TTCGAA 1 cut(s) 704
Bsp1286I GDGCHC 2 cut(s) 493, 608
Bsp143I GATC 5 cut(s) 70, 253, 325, 622, 670
BspANI GGCC 2 cut(s) 137, 558
BspCNI CTCAG 3 cut(s) 67, 196, 442
BspFNI CGCG 1 cut(s) 426
BspPI GGATC 2 cut(s) 65, 261
BspQI GCTCTTC 2 cut(s) 240, 613
BspT104I TTCGAA 1 cut(s) 704
BsrDI GCAATG 2 cut(s) 537, 600
BsrFI RCCGGY 1 cut(s) 15
BsrI ACTGG 3 cut(s) 215, 559, 699
BssAI RCCGGY 1 cut(s) 15
BssMI GATC 5 cut(s) 70, 253, 325, 622, 670
Bst2UI CCWGG 1 cut(s) 681
Bst4CI ACNGT 2 cut(s) 648, 691
Bst6I CTCTTC 4 cut(s) 240, 269, 510, 613
BstAPI GCANNNNNTGC 1 cut(s) 443
BstBAI YACGTR 1 cut(s) 263
BstBI TTCGAA 1 cut(s) 704
BstC8I GCNNGC 2 cut(s) 93, 424
BstDEI CTNAG 4 cut(s) 75, 204, 429, 526
BstFNI CGCG 1 cut(s) 426
BstHHI GCGC 2 cut(s) 193, 428
BstKTI GATC 5 cut(s) 73, 256, 328, 625, 673
BstMBI GATC 5 cut(s) 70, 253, 325, 622, 670
BstMWI GCNNNNNNNGC 6 cut(s) 197, 230, 266, 434, 443, 497
BstNI CCWGG 1 cut(s) 681
BstSCI CCNGG 1 cut(s) 679
BstSFI CTRYAG 1 cut(s) 567
BstSLI GKGCMC 1 cut(s) 493
BstUI CGCG 1 cut(s) 426
BstV1I GCAGC 1 cut(s) 175
BstX2I RGATCY 3 cut(s) 70, 253, 670
BstYI RGATCY 3 cut(s) 70, 253, 670
BsuRI GGCC 2 cut(s) 137, 558
Cac8I GCNNGC 2 cut(s) 93, 424
CfoI GCGC 2 cut(s) 193, 428
Cfr10I RCCGGY 1 cut(s) 15
CseI GACGC 1 cut(s) 119
CspAI ACCGGT 1 cut(s) 15
DdeI CTNAG 4 cut(s) 75, 204, 429, 526
DpnI GATC 5 cut(s) 72, 255, 327, 624, 672
DpnII GATC 5 cut(s) 70, 253, 325, 622, 670
EaeI YGGCCR 1 cut(s) 556
Eam1104I CTCTTC 4 cut(s) 240, 269, 510, 613
EarI CTCTTC 4 cut(s) 240, 269, 510, 613
Ecl136II GAGCTC 1 cut(s) 606
Eco24I GRGCYC 1 cut(s) 608
Eco53kI GAGCTC 1 cut(s) 606
Eco57I CTGAAG 2 cut(s) 221, 293
Eco72I CACGTG 1 cut(s) 263
EcoICRI GAGCTC 1 cut(s) 606
EcoRII CCWGG 1 cut(s) 679
EcoT38I GRGCYC 1 cut(s) 608
FaiI YATR 6 cut(s) 40, 42, 56, 183, 311, 715
FalI AAGNNNNNCTT 4 cut(s) 84, 116, 657, 689
FauNDI CATATG 1 cut(s) 40
FbaI TGATCA 1 cut(s) 325
Fnu4HI GCNGC 1 cut(s) 189
FriOI GRGCYC 1 cut(s) 608
Fsp4HI GCNGC 1 cut(s) 189
FspBI CTAG 2 cut(s) 24, 599
FspI TGCGCA 1 cut(s) 192
GlaI GCGC 2 cut(s) 192, 427
GluI GCNGC 1 cut(s) 189
HaeIII GGCC 2 cut(s) 137, 558
HapII CCGG 1 cut(s) 16
HgaI GACGC 1 cut(s) 119
HhaI GCGC 2 cut(s) 193, 428
Hin6I GCGC 2 cut(s) 191, 426
HinP1I GCGC 2 cut(s) 191, 426
HincII GTYRAC 1 cut(s) 564
HindII GTYRAC 1 cut(s) 564
HindIII AAGCTT 2 cut(s) 198, 368
HinfI GANTC 2 cut(s) 536, 728
HpaI GTTAAC 1 cut(s) 564
HpaII CCGG 1 cut(s) 16
Hpy166II GTNNAC 1 cut(s) 564
Hpy188I TCNGA 4 cut(s) 205, 240, 273, 733
Hpy188III TCNNGA 3 cut(s) 24, 74, 101
Hpy8I GTNNAC 1 cut(s) 564
HpyAV CCTTC 2 cut(s) 575, 756
HpyCH4III ACNGT 2 cut(s) 648, 691
HpyCH4IV ACGT 2 cut(s) 166, 262
HpyCH4V TGCA 6 cut(s) 44, 50, 260, 437, 593, 629
HpyF10VI GCNNNNNNNGC 6 cut(s) 197, 230, 266, 434, 443, 497
HpyF3I CTNAG 4 cut(s) 75, 204, 429, 526
HpySE526I ACGT 2 cut(s) 166, 262
HspAI GCGC 2 cut(s) 191, 426
Ksp22I TGATCA 1 cut(s) 325
KspAI GTTAAC 1 cut(s) 564
Kzo9I GATC 5 cut(s) 70, 253, 325, 622, 670
LguI GCTCTTC 2 cut(s) 240, 613
LmnI GCTCC 2 cut(s) 88, 745
LpnPI CCDG 9 cut(s) 29, 87, 119, 196, 572, 636, 666, 693, 712
Lsp1109I GCAGC 1 cut(s) 175
LweI GCATC 1 cut(s) 117
MaeI CTAG 2 cut(s) 24, 599
MaeII ACGT 2 cut(s) 166, 262
MaeIII GTNAC 1 cut(s) 691
MalI GATC 5 cut(s) 72, 255, 327, 624, 672
MboI GATC 5 cut(s) 70, 253, 325, 622, 670
MboII GAAGA 8 cut(s) 80, 227, 286, 527, 600, 649, 665, 696
MflI RGATCY 3 cut(s) 70, 253, 670
MhlI GDGCHC 2 cut(s) 493, 608
MlsI TGGCCA 1 cut(s) 558
MluCI AATT 4 cut(s) 227, 304, 735, 755
MluNI TGGCCA 1 cut(s) 558
MnlI CCTC 7 cut(s) 199, 241, 244, 270, 286, 385, 425
Mox20I TGGCCA 1 cut(s) 558
MscI TGGCCA 1 cut(s) 558
MseI TTAA 2 cut(s) 563, 750
Msp20I TGGCCA 1 cut(s) 558
MspI CCGG 1 cut(s) 16
MspR9I CCNGG 1 cut(s) 681
MvaI CCWGG 1 cut(s) 681
MvnI CGCG 1 cut(s) 426
MwoI GCNNNNNNNGC 6 cut(s) 197, 230, 266, 434, 443, 497
NdeI CATATG 1 cut(s) 40
NdeII GATC 5 cut(s) 70, 253, 325, 622, 670
NsbI TGCGCA 1 cut(s) 192
NspV TTCGAA 1 cut(s) 704
PciSI GCTCTTC 2 cut(s) 240, 613
PfeI GAWTC 2 cut(s) 536, 728
PinAI ACCGGT 1 cut(s) 15
PkrI GCNGC 1 cut(s) 190
PmaCI CACGTG 1 cut(s) 263
PmlI CACGTG 1 cut(s) 263
Ppu21I YACGTR 1 cut(s) 263
Psp124BI GAGCTC 1 cut(s) 608
Psp1406I AACGTT 1 cut(s) 166
Psp6I CCWGG 1 cut(s) 679
PspCI CACGTG 1 cut(s) 263
PspGI CCWGG 1 cut(s) 679
PsuI RGATCY 3 cut(s) 70, 253, 670
SacI GAGCTC 1 cut(s) 608
SapI GCTCTTC 2 cut(s) 240, 613
SaqAI TTAA 2 cut(s) 563, 750
SatI GCNGC 1 cut(s) 189
Sau3AI GATC 5 cut(s) 70, 253, 325, 622, 670
ScrFI CCNGG 1 cut(s) 681
SduI GDGCHC 2 cut(s) 493, 608
SfaNI GCATC 1 cut(s) 117
SfcI CTRYAG 1 cut(s) 567
SfuI TTCGAA 1 cut(s) 704
Sse9I AATT 4 cut(s) 227, 304, 735, 755
SspMI CTAG 2 cut(s) 24, 599
SstI GAGCTC 1 cut(s) 608
StyD4I CCNGG 1 cut(s) 679
TaaI ACNGT 2 cut(s) 648, 691
TaiI ACGT 2 cut(s) 169, 265
TaqI TCGA 3 cut(s) 217, 401, 704
TasI AATT 4 cut(s) 227, 304, 735, 755
TfiI GAWTC 2 cut(s) 536, 728
Tru1I TTAA 2 cut(s) 563, 750
Tru9I TTAA 2 cut(s) 563, 750
TseI GCWGC 1 cut(s) 188
TspDTI ATGAA 3 cut(s) 17, 122, 768
XbaI TCTAGA 1 cut(s) 23
XspI CTAG 2 cut(s) 24, 599
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.