MD15G1434500.v1.1

Membrane-associated 30 kDa protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0002961
Physical Location & Seq
Forward (+)
53481231 .. 53481682
452 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1434500.v1.1.491

Sequence Viewer

Length: 348 bp
ATGAATCTATTTGACCGGTTTGCTAGAGTTGTCAAGTCATATGCAAATACATTAATAAGTACCTTTGAAGATCCTAAGAAAATCTTAGAGCAAACAGTTCTTGAAATGAATGATGACTTGACAAAGATGCGTCAGGCCACAGCACAAGTGTTGGCATCTCAAAAGCGGATGGAGAATAAGTACAAAGCTGCTGAACAAGCTTCTGAGGACTGGTACAGCAAATCACAATTAGCTCTTCAAAAAGGAGAGGATGATCTTGCACGTGAGGCTCTGAAGAGGCATACAAGAGGCAAGGTCGAAAAAAGATACCTTCAAAGCACGTGCTCAGTCTGCGAAGACTGCAACTAA

Protein Analysis

116

Amino Acids

13.33

Weight (kDa)

8.42

Isoelectric Point (pI)

47.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PspA_IM30 PF04012 2 - 95 2.5e-27 PspA/IM30 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 166
AclWI GGATC 1 cut(s) 65
AcuI CTGAAG 1 cut(s) 293
AcvI CACGTG 2 cut(s) 263, 321
AfaI GTAC 3 cut(s) 61, 182, 215
AgeI ACCGGT 1 cut(s) 15
AgsI TTSAA 4 cut(s) 68, 104, 239, 314
AluBI AGCT 3 cut(s) 188, 200, 233
AluI AGCT 3 cut(s) 188, 200, 233
Alw21I GWGCWC 1 cut(s) 326
AlwI GGATC 1 cut(s) 65
AoxI GGCC 1 cut(s) 135
ApeKI GCWGC 1 cut(s) 188
AseI ATTAAT 1 cut(s) 53
AsiGI ACCGGT 1 cut(s) 15
BbrPI CACGTG 2 cut(s) 263, 321
BbsI GAAGAC 1 cut(s) 342
Bbv12I GWGCWC 1 cut(s) 326
BbvI GCAGC 1 cut(s) 175
BccI CCATC 1 cut(s) 163
BfaI CTAG 1 cut(s) 24
BisI GCNGC 1 cut(s) 189
BlsI GCNGC 1 cut(s) 190
BmsI GCATC 2 cut(s) 117, 164
BpiI GAAGAC 1 cut(s) 342
BsaAI YACGTR 2 cut(s) 263, 321
BsaWI WCCGGW 1 cut(s) 15
BsaXI ACNNNNNCTCC 2 cut(s) 164, 194
Bse118I RCCGGY 1 cut(s) 15
Bse1I ACTGG 1 cut(s) 215
BseGI GGATG 2 cut(s) 174, 256
BseMII CTCAG 2 cut(s) 195, 339
BseNI ACTGG 1 cut(s) 215
BseXI GCAGC 1 cut(s) 175
BshFI GGCC 1 cut(s) 137
BshTI ACCGGT 1 cut(s) 15
BsiHKAI GWGCWC 1 cut(s) 326
BsiSI CCGG 1 cut(s) 16
BsnI GGCC 1 cut(s) 137
Bsp1286I GDGCHC 1 cut(s) 326
Bsp143I GATC 2 cut(s) 70, 253
BspACI CCGC 1 cut(s) 166
BspANI GGCC 1 cut(s) 137
BspCNI CTCAG 2 cut(s) 196, 338
BspPI GGATC 1 cut(s) 65
BspQI GCTCTTC 1 cut(s) 240
BsrFI RCCGGY 1 cut(s) 15
BsrI ACTGG 1 cut(s) 215
BssAI RCCGGY 1 cut(s) 15
BssMI GATC 2 cut(s) 70, 253
Bst4CI ACNGT 1 cut(s) 97
Bst6I CTCTTC 2 cut(s) 240, 269
BstBAI YACGTR 2 cut(s) 263, 321
BstDEI CTNAG 4 cut(s) 75, 85, 204, 325
BstF5I GGATG 2 cut(s) 174, 256
BstKTI GATC 2 cut(s) 73, 256
BstMBI GATC 2 cut(s) 70, 253
BstMWI GCNNNNNNNGC 4 cut(s) 197, 266, 330, 339
BstV1I GCAGC 1 cut(s) 175
BstV2I GAAGAC 1 cut(s) 342
BstX2I RGATCY 1 cut(s) 70
BstYI RGATCY 1 cut(s) 70
BsuRI GGCC 1 cut(s) 137
BtsCI GGATG 2 cut(s) 174, 256
Cfr10I RCCGGY 1 cut(s) 15
CseI GACGC 1 cut(s) 119
Csp6I GTAC 3 cut(s) 60, 181, 214
CspAI ACCGGT 1 cut(s) 15
CviJI RGCY 5 cut(s) 137, 188, 200, 233, 269
CviKI_1 RGCY 5 cut(s) 137, 188, 200, 233, 269
CviQI GTAC 3 cut(s) 60, 181, 214
DdeI CTNAG 4 cut(s) 75, 85, 204, 325
DpnI GATC 2 cut(s) 72, 255
DpnII GATC 2 cut(s) 70, 253
Eam1104I CTCTTC 2 cut(s) 240, 269
EarI CTCTTC 2 cut(s) 240, 269
Eco57I CTGAAG 1 cut(s) 293
Eco72I CACGTG 2 cut(s) 263, 321
FaiI YATR 3 cut(s) 40, 42, 282
FalI AAGNNNNNCTT 2 cut(s) 68, 100
FauNDI CATATG 1 cut(s) 40
Fnu4HI GCNGC 1 cut(s) 189
FokI GGATG 2 cut(s) 181, 263
Fsp4HI GCNGC 1 cut(s) 189
FspBI CTAG 1 cut(s) 24
GluI GCNGC 1 cut(s) 189
HaeIII GGCC 1 cut(s) 137
HapII CCGG 1 cut(s) 16
HgaI GACGC 1 cut(s) 119
HindIII AAGCTT 1 cut(s) 198
HinfI GANTC 1 cut(s) 4
HpaII CCGG 1 cut(s) 16
Hpy188I TCNGA 2 cut(s) 205, 273
Hpy188III TCNNGA 1 cut(s) 101
HpyAV CCTTC 1 cut(s) 320
HpyCH4III ACNGT 1 cut(s) 97
HpyCH4IV ACGT 2 cut(s) 262, 320
HpyCH4V TGCA 3 cut(s) 44, 260, 342
HpyF10VI GCNNNNNNNGC 4 cut(s) 197, 266, 330, 339
HpyF3I CTNAG 4 cut(s) 75, 85, 204, 325
HpySE526I ACGT 2 cut(s) 262, 320
Kzo9I GATC 2 cut(s) 70, 253
LguI GCTCTTC 1 cut(s) 240
LpnPI CCDG 3 cut(s) 29, 119, 196
Lsp1109I GCAGC 1 cut(s) 175
LweI GCATC 2 cut(s) 117, 164
MaeI CTAG 1 cut(s) 24
MaeII ACGT 2 cut(s) 262, 320
MalI GATC 2 cut(s) 72, 255
MboI GATC 2 cut(s) 70, 253
MboII GAAGA 4 cut(s) 80, 227, 286, 347
MflI RGATCY 1 cut(s) 70
MhlI GDGCHC 1 cut(s) 326
MluCI AATT 1 cut(s) 227
MnlI CCTC 5 cut(s) 199, 241, 259, 270, 281
MseI TTAA 1 cut(s) 53
MspI CCGG 1 cut(s) 16
MwoI GCNNNNNNNGC 4 cut(s) 197, 266, 330, 339
NdeI CATATG 1 cut(s) 40
NdeII GATC 2 cut(s) 70, 253
PciSI GCTCTTC 1 cut(s) 240
PfeI GAWTC 1 cut(s) 4
PinAI ACCGGT 1 cut(s) 15
PkrI GCNGC 1 cut(s) 190
PmaCI CACGTG 2 cut(s) 263, 321
PmlI CACGTG 2 cut(s) 263, 321
Ppu21I YACGTR 2 cut(s) 263, 321
PshBI ATTAAT 1 cut(s) 53
PspCI CACGTG 2 cut(s) 263, 321
PsuI RGATCY 1 cut(s) 70
RsaI GTAC 3 cut(s) 61, 182, 215
RsaNI GTAC 3 cut(s) 60, 181, 214
SapI GCTCTTC 1 cut(s) 240
SaqAI TTAA 1 cut(s) 53
SatI GCNGC 1 cut(s) 189
Sau3AI GATC 2 cut(s) 70, 253
SduI GDGCHC 1 cut(s) 326
SetI ASST 8 cut(s) 65, 190, 202, 235, 265, 297, 312, 323
SfaNI GCATC 2 cut(s) 117, 164
Sse9I AATT 1 cut(s) 227
SsiI CCGC 1 cut(s) 166
SspMI CTAG 1 cut(s) 24
TaaI ACNGT 1 cut(s) 97
TaiI ACGT 2 cut(s) 265, 323
TaqI TCGA 1 cut(s) 297
TasI AATT 1 cut(s) 227
TatI WGTACW 1 cut(s) 180
TfiI GAWTC 1 cut(s) 4
Tru1I TTAA 1 cut(s) 53
Tru9I TTAA 1 cut(s) 53
TseI GCWGC 1 cut(s) 188
TspDTI ATGAA 2 cut(s) 17, 122
VspI ATTAAT 1 cut(s) 53
XspI CTAG 1 cut(s) 24
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.