Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr00

Orthogroup ID

OG0002961

Physical Location & Seq

Genomic Coordinates Forward (+)

3416233 .. 3432250

Gene Structure 16018 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD00G1019200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1008 bp

ATGGCCACAAAATGGCAGATATTTACAGGATTGAGCATGGCGTCGACGCCTGCCTCTTCTTTCCAAGCTTCTTCTTCGAGCTCTAGCGGCCTCTGCATGGTCAGAAAACCTCTCACGACTTCCTTCTTCAATGGCGGAGTGGAGGCTCTAAAAGTTGAAGTGATTAGGGTTGCTCCTTCCAGTCGACCACATTATTCTAGACAACGTGGAGGGGCTCTTGGTGCTCGTATGAATCTATTTGACCGGTTTGCTAGAGTTGTCAAGTCATATGCAAATGCATTAATAAGTACCTTTGAAGATCCTGAGAAAATCTTAGAGCAAACAGTTCTTGAAATGAATGATGACTTGACAAAGATGCGTCAGGCCACAGCACAAGTGTTGGCATCTCAAAAGCGGATGGAGAATAAGTACAAAGCTGCTGAACAAGCTTCTGAGGACTGGTACGGCAAAGCACAATTAGCTCTTCAAAAAGGAGAGGAGGATCTTGCACGTGAGGCTCTGAAGAGGCGTAAATCTTTTGCTGATAATGCTAATACTTTGAAATCTCAACTTGATCAACAAAAAGCTGTTGTCGATAATCTTGTGTCTAATACACGGCTTTTAGAAAGCAAGATACAGGAGGCAAGGTCGAAAAAAGATACCCTCAAAGCACGTGCTCAGTCTGCGAAGACTGCAACTAAAGTGAGTGAGATGGTGGGAAACGTCAAGACAAGTAGTGCTCTTTCAGCTTTTGATAAGATGGAAGAGAAAGTCTTAGCCATGGAATCCCAAGCAGAAGCTCTTGGTCAGTTAACTACGGATGATCTGGAAGGAAAGTTTGCGTTGCTAGAGGGATCTTCGGTCGACGATGATCTTGCAAACTTGAAGAAAGAATTATCTGGTAGTTCAAAGAAAGGAGAGCTCCCGCCTGGAAGAACAGATGCTTCCAGTAACAAGGCATATCCATTTCGAGATTCTGAAATTGAGATGGAGCTTAATGAATTGAGAAGAAAAGCCAAGGACTTCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006996 GO:0008150 GO:0009507 GO:0009534 GO:0009535 GO:0009536 GO:0009579 GO:0009657 GO:0009668 GO:0009987 GO:0010027 GO:0016020 GO:0016043 GO:0031976 GO:0031984 GO:0034357 GO:0042651 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044436 GO:0044444 GO:0044446 GO:0044464 GO:0055035 GO:0061024 GO:0071840

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PspA_IM30

Protein Analysis

336

Amino Acids

36.93

Weight (kDa)

9.13

Isoelectric Point (pI)

45.7

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PspA_IM30	PF04012	78 - 291	1.1e-52	PspA/IM30 family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002961)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G65260
fragaria_vesca	FvH4_1g24800 FvH4_1g24800 FvH4_2g19990 FvH4_2g19990 FvH4_3g16870
malus_domestica	MD00G1019200.v1.1 MD15G1434500.v1.1 MD17G1177400.v1.1
prunus_persica	Prupe.1G203200_v2.0.a1
pyrus_communis	pycom13g26650 pycom17g18630
rosa_chinensis	RchiOBHm_Chr2g0122441 RchiOBHm_Chr5g0028111 RchiOBHm_Chr6g0285711
rosa_laevigata	RLG00000012615 RLG00000018677 RLG00000033067
rosa_multiflora	Rmu_sc0001356.1_g000011 Rmu_sc0001989.1_g000001 Rmu_sc0003832.1_g000020
rosa_roxburghii	Rroxscaffold_2G00121650 Rroxscaffold_7G00182810
rosa_rugosa	Rorug02G0237700 Rorug02G0237800 Rorug05G0104200 Rorug06G0176600 Rorug06G0176700
rosa_samantha	Rh5BG196000 Rh5CG216000 Rh5DG197700 Rh6AG288200 Rh6BG289800 Rh6CG290800 Rh6DG283500
rosa_wichuraiana	Rw2G023650 Rw5G018030 Rw6G024760

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	1 cut(s)	12
AccI	GTMKAC	3 cut(s)	44, 184, 843
AciI	CCGC	4 cut(s)	87, 135, 394, 905
AclWI	GGATC	3 cut(s)	293, 489, 841
AcoI	YGGCCR	1 cut(s)	3
AcuI	CTGAAG	1 cut(s)	521
AcvI	CACGTG	2 cut(s)	491, 653
AcyI	GRCGYC	2 cut(s)	41, 47
AfaI	GTAC	3 cut(s)	289, 410, 443
AfiI	CCNNNNNNNGG	2 cut(s)	12, 97
AgeI	ACCGGT	1 cut(s)	243
AgsI	TTSAA	8 cut(s)	130, 158, 296, 332, 467, 541, 865, 888
AjnI	CCWGG	1 cut(s)	907
Alw21I	GWGCWC	5 cut(s)	83, 226, 658, 721, 903
AlwI	GGATC	3 cut(s)	293, 489, 841
AoxI	GGCC	3 cut(s)	3, 88, 363
ApeKI	GCWGC	1 cut(s)	416
AseI	ATTAAT	1 cut(s)	281
AsiGI	ACCGGT	1 cut(s)	243
BalI	TGGCCA	1 cut(s)	5
BanII	GRGCYC	3 cut(s)	83, 217, 903
BbrPI	CACGTG	2 cut(s)	491, 653
BbsI	GAAGAC	1 cut(s)	674
Bbv12I	GWGCWC	5 cut(s)	83, 226, 658, 721, 903
BbvI	GCAGC	1 cut(s)	403
BccI	CCATC	4 cut(s)	391, 685, 733, 961
BceAI	ACGGC	2 cut(s)	460, 611
BcgI	CGANNNNNNTGC	2 cut(s)	836, 870
BciT130I	CCWGG	1 cut(s)	909
BclI	TGATCA	1 cut(s)	553
BfaI	CTAG	4 cut(s)	84, 198, 252, 827
BisI	GCNGC	2 cut(s)	88, 417
BlsI	GCNGC	2 cut(s)	89, 418
Bme1390I	CCNGG	1 cut(s)	909
BmrFI	CCNGG	1 cut(s)	909
BmsI	GCATC	3 cut(s)	345, 392, 910
BpiI	GAAGAC	1 cut(s)	674
BsaAI	YACGTR	2 cut(s)	491, 653
BsaHI	GRCGYC	2 cut(s)	41, 47
BsaJI	CCNNGG	2 cut(s)	759, 996
BsaWI	WCCGGW	1 cut(s)	243
BsaXI	ACNNNNNCTCC	4 cut(s)	392, 422, 611, 641
Bsc4I	CCNNNNNNNGG	2 cut(s)	12, 97
Bse118I	RCCGGY	1 cut(s)	243
Bse1I	ACTGG	3 cut(s)	180, 443, 927
BseBI	CCWGG	1 cut(s)	909
BseDI	CCNNGG	2 cut(s)	759, 996
BseGI	GGATG	2 cut(s)	402, 805
BseLI	CCNNNNNNNGG	2 cut(s)	12, 97
BseMII	CTCAG	3 cut(s)	294, 423, 671
BseNI	ACTGG	3 cut(s)	180, 443, 927
BseRI	GAGGAG	1 cut(s)	491
BseXI	GCAGC	1 cut(s)	403
Bsh1285I	CGRYCG	1 cut(s)	843
BshFI	GGCC	3 cut(s)	5, 90, 365
BshTI	ACCGGT	1 cut(s)	243
BsiEI	CGRYCG	1 cut(s)	843
BsiHKAI	GWGCWC	5 cut(s)	83, 226, 658, 721, 903
BsiSI	CCGG	1 cut(s)	244
BslI	CCNNNNNNNGG	2 cut(s)	12, 97
BsnI	GGCC	3 cut(s)	5, 90, 365
Bsp1286I	GDGCHC	6 cut(s)	83, 217, 226, 658, 721, 903
Bsp143I	GATC	6 cut(s)	298, 481, 553, 802, 833, 850
Bsp19I	CCATGG	1 cut(s)	759
BspACI	CCGC	4 cut(s)	87, 135, 394, 905
BspANI	GGCC	3 cut(s)	5, 90, 365
BspCNI	CTCAG	3 cut(s)	295, 424, 670
BspPI	GGATC	3 cut(s)	293, 489, 841
BspQI	GCTCTTC	1 cut(s)	468
BsrFI	RCCGGY	1 cut(s)	243
BsrI	ACTGG	3 cut(s)	180, 443, 927
BssAI	RCCGGY	1 cut(s)	243
BssECI	CCNNGG	2 cut(s)	759, 996
BssMI	GATC	6 cut(s)	298, 481, 553, 802, 833, 850
BssNI	GRCGYC	2 cut(s)	41, 47
BssT1I	CCWWGG	2 cut(s)	759, 996
Bst2UI	CCWGG	1 cut(s)	909
Bst4CI	ACNGT	1 cut(s)	325
Bst6I	CTCTTC	4 cut(s)	61, 468, 497, 738
BstACI	GRCGYC	2 cut(s)	41, 47
BstBAI	YACGTR	2 cut(s)	491, 653
BstC8I	GCNNGC	1 cut(s)	51
BstDEI	CTNAG	5 cut(s)	303, 313, 432, 657, 754
BstDSI	CCRYGG	1 cut(s)	759
BstF5I	GGATG	2 cut(s)	402, 805
BstKTI	GATC	6 cut(s)	301, 484, 556, 805, 836, 853
BstMBI	GATC	6 cut(s)	298, 481, 553, 802, 833, 850
BstMCI	CGRYCG	1 cut(s)	843
BstNI	CCWGG	1 cut(s)	909
BstSCI	CCNGG	1 cut(s)	907
BstV1I	GCAGC	1 cut(s)	403
BstV2I	GAAGAC	1 cut(s)	674
BstX2I	RGATCY	3 cut(s)	298, 481, 833
BstYI	RGATCY	3 cut(s)	298, 481, 833
BsuRI	GGCC	3 cut(s)	5, 90, 365
BtgI	CCRYGG	1 cut(s)	759
BtsCI	GGATG	2 cut(s)	402, 805
Cac8I	GCNNGC	1 cut(s)	51
Cfr10I	RCCGGY	1 cut(s)	243
CseI	GACGC	3 cut(s)	30, 55, 347
Csp6I	GTAC	3 cut(s)	288, 409, 442
CspAI	ACCGGT	1 cut(s)	243
CviAII	CATG	3 cut(s)	37, 97, 760
CviQI	GTAC	3 cut(s)	288, 409, 442
DdeI	CTNAG	5 cut(s)	303, 313, 432, 657, 754
DpnI	GATC	6 cut(s)	300, 483, 555, 804, 835, 852
DpnII	GATC	6 cut(s)	298, 481, 553, 802, 833, 850
EaeI	YGGCCR	1 cut(s)	3
Eam1104I	CTCTTC	4 cut(s)	61, 468, 497, 738
EarI	CTCTTC	4 cut(s)	61, 468, 497, 738
EciI	GGCGGA	1 cut(s)	150
Ecl136II	GAGCTC	2 cut(s)	81, 901
Eco130I	CCWWGG	2 cut(s)	759, 996
Eco24I	GRGCYC	3 cut(s)	83, 217, 903
Eco53kI	GAGCTC	2 cut(s)	81, 901
Eco57I	CTGAAG	1 cut(s)	521
Eco72I	CACGTG	2 cut(s)	491, 653
EcoICRI	GAGCTC	2 cut(s)	81, 901
EcoRII	CCWGG	1 cut(s)	907
EcoT14I	CCWWGG	2 cut(s)	759, 996
EcoT22I	ATGCAT	1 cut(s)	280
EcoT38I	GRGCYC	3 cut(s)	83, 217, 903
ErhI	CCWWGG	2 cut(s)	759, 996
FaeI	CATG	3 cut(s)	40, 100, 763
FaiI	YATR	7 cut(s)	38, 98, 230, 268, 270, 761, 940
FatI	CATG	3 cut(s)	36, 96, 759
FauI	CCCGC	1 cut(s)	912
FauNDI	CATATG	1 cut(s)	268
FbaI	TGATCA	1 cut(s)	553
FblI	GTMKAC	3 cut(s)	44, 184, 843
Fnu4HI	GCNGC	2 cut(s)	88, 417
FokI	GGATG	2 cut(s)	409, 812
FriOI	GRGCYC	3 cut(s)	83, 217, 903
Fsp4HI	GCNGC	2 cut(s)	88, 417
FspBI	CTAG	4 cut(s)	84, 198, 252, 827
GluI	GCNGC	2 cut(s)	88, 417
HaeIII	GGCC	3 cut(s)	5, 90, 365
HapII	CCGG	1 cut(s)	244
HgaI	GACGC	3 cut(s)	30, 55, 347
Hin1I	GRCGYC	2 cut(s)	41, 47
Hin1II	CATG	3 cut(s)	40, 100, 763
HincII	GTYRAC	4 cut(s)	45, 185, 792, 844
HindII	GTYRAC	4 cut(s)	45, 185, 792, 844
HindIII	AAGCTT	2 cut(s)	66, 426
HinfI	GANTC	3 cut(s)	232, 764, 953
HpaI	GTTAAC	1 cut(s)	792
HpaII	CCGG	1 cut(s)	244
Hpy166II	GTNNAC	4 cut(s)	45, 185, 792, 844
Hpy188I	TCNGA	4 cut(s)	104, 433, 501, 958
Hpy188III	TCNNGA	7 cut(s)	115, 198, 302, 329, 706, 806, 950
Hpy8I	GTNNAC	4 cut(s)	45, 185, 792, 844
Hpy99I	CGWCG	3 cut(s)	46, 49, 848
HpyAV	CCTTC	3 cut(s)	133, 186, 803
HpyCH4III	ACNGT	1 cut(s)	325
HpyCH4IV	ACGT	4 cut(s)	205, 490, 652, 702
HpyCH4V	TGCA	6 cut(s)	96, 272, 278, 488, 674, 857
HpyF3I	CTNAG	5 cut(s)	303, 313, 432, 657, 754
HpySE526I	ACGT	4 cut(s)	205, 490, 652, 702
Hsp92I	GRCGYC	2 cut(s)	41, 47
Hsp92II	CATG	3 cut(s)	40, 100, 763
Ksp22I	TGATCA	1 cut(s)	553
KspAI	GTTAAC	1 cut(s)	792
Kzo9I	GATC	6 cut(s)	298, 481, 553, 802, 833, 850
LguI	GCTCTTC	1 cut(s)	468
LmnI	GCTCC	3 cut(s)	178, 906, 970
Lsp1109I	GCAGC	1 cut(s)	403
LweI	GCATC	3 cut(s)	345, 392, 910
MaeI	CTAG	4 cut(s)	84, 198, 252, 827
MaeII	ACGT	4 cut(s)	205, 490, 652, 702
MaeIII	GTNAC	1 cut(s)	929
MalI	GATC	6 cut(s)	300, 483, 555, 804, 835, 852
MboI	GATC	6 cut(s)	298, 481, 553, 802, 833, 850
MflI	RGATCY	3 cut(s)	298, 481, 833
MhlI	GDGCHC	6 cut(s)	83, 217, 226, 658, 721, 903
MlsI	TGGCCA	1 cut(s)	5
MluCI	AATT	4 cut(s)	455, 872, 960, 980
MluNI	TGGCCA	1 cut(s)	5
Mox20I	TGGCCA	1 cut(s)	5
Mph1103I	ATGCAT	1 cut(s)	280
MscI	TGGCCA	1 cut(s)	5
MseI	TTAA	3 cut(s)	281, 791, 975
Msp20I	TGGCCA	1 cut(s)	5
MspI	CCGG	1 cut(s)	244
MspR9I	CCNGG	1 cut(s)	909
MvaI	CCWGG	1 cut(s)	909
NcoI	CCATGG	1 cut(s)	759
NdeI	CATATG	1 cut(s)	268
NdeII	GATC	6 cut(s)	298, 481, 553, 802, 833, 850
NlaIII	CATG	3 cut(s)	40, 100, 763
NsiI	ATGCAT	1 cut(s)	280
PciSI	GCTCTTC	1 cut(s)	468
PfeI	GAWTC	3 cut(s)	232, 764, 953
PflMI	CCANNNNNTGG	1 cut(s)	12
PinAI	ACCGGT	1 cut(s)	243
PkrI	GCNGC	2 cut(s)	89, 418
PmaCI	CACGTG	2 cut(s)	491, 653
PmlI	CACGTG	2 cut(s)	491, 653
Ppu21I	YACGTR	2 cut(s)	491, 653
PshBI	ATTAAT	1 cut(s)	281
Psp124BI	GAGCTC	2 cut(s)	83, 903
Psp6I	CCWGG	1 cut(s)	907
PspCI	CACGTG	2 cut(s)	491, 653
PspGI	CCWGG	1 cut(s)	907
PsuI	RGATCY	3 cut(s)	298, 481, 833
RsaI	GTAC	3 cut(s)	289, 410, 443
RsaNI	GTAC	3 cut(s)	288, 409, 442
SacI	GAGCTC	2 cut(s)	83, 903
SalI	GTCGAC	3 cut(s)	43, 183, 842
SapI	GCTCTTC	1 cut(s)	468
SaqAI	TTAA	3 cut(s)	281, 791, 975
SatI	GCNGC	2 cut(s)	88, 417
Sau3AI	GATC	6 cut(s)	298, 481, 553, 802, 833, 850
ScrFI	CCNGG	1 cut(s)	909
SduI	GDGCHC	6 cut(s)	83, 217, 226, 658, 721, 903
SfaNI	GCATC	3 cut(s)	345, 392, 910
SgrDI	CGTCGACG	1 cut(s)	43
Sse9I	AATT	4 cut(s)	455, 872, 960, 980
SsiI	CCGC	4 cut(s)	87, 135, 394, 905
SspMI	CTAG	4 cut(s)	84, 198, 252, 827
SstI	GAGCTC	2 cut(s)	83, 903
StyD4I	CCNGG	1 cut(s)	907
StyI	CCWWGG	2 cut(s)	759, 996
TaaI	ACNGT	1 cut(s)	325
TaiI	ACGT	4 cut(s)	208, 493, 655, 705
TaqI	TCGA	7 cut(s)	44, 77, 184, 573, 629, 843, 949
TaqII	GACCGA	1 cut(s)	829
TasI	AATT	4 cut(s)	455, 872, 960, 980
TatI	WGTACW	1 cut(s)	408
TauI	GCSGC	1 cut(s)	90
TfiI	GAWTC	3 cut(s)	232, 764, 953
Tru1I	TTAA	3 cut(s)	281, 791, 975
Tru9I	TTAA	3 cut(s)	281, 791, 975
TseI	GCWGC	1 cut(s)	416
TspDTI	ATGAA	3 cut(s)	245, 350, 993
TspGWI	ACGGA	1 cut(s)	812
Van91I	CCANNNNNTGG	1 cut(s)	12
VspI	ATTAAT	1 cut(s)	281
XbaI	TCTAGA	1 cut(s)	197
XmiI	GTMKAC	3 cut(s)	44, 184, 843
XspI	CTAG	4 cut(s)	84, 198, 252, 827
Zsp2I	ATGCAT	1 cut(s)	280

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.