FvH4_3g16870

Membrane-associated 30 kDa protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0002961
Physical Location & Seq
Reverse (-)
10653737 .. 10654720
984 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g16870.t1

Sequence Viewer

Length: 984 bp
ATGATGGCCGCTAATTCGCAGATTTTTATAGGAATGACCATTCCATCAATGCTTGCAGCCTCTTCATCTTCATCCTCCACCGGTCTCTCTATGGTAAGGAGACCTCTCACAACGTCGTTCTATAACGCCGGGGTGGGAGCTCTAAAACTTGAAACGATTAGGGTTGCTCCTTCCAATAAGCAAAACCATTACAAACATCGTGGAACAGCTCTTGTTGCCCGTATGAACCTTTTCGACCGGTTTGCTATGGTTATCAAGTCATATGCAAATGCAATCATGTCTTCGTTTGAAGATCCTGAGAAAATGTTAGAGCAAGCAGTTCTCGAAATGAAAGATGATCTGGCCAAGATGCGACAGGCCTCAGCACAAGCAATGGCATCTCGAAAAATGCTGGAAAACAAGTATGAAGCTGCGAAACGGGCTTCAGAGGATTGGTACCGAAAACAAGGAGAGGAAGATTTAGCACGTGAAGCTCTCAAGAGGCGAATATCTCTTGCGGATAATGCTAGTTCTCTGAGATCACAACTTGATCAACAGAAGGGTGCTTTTGATAGTCTTGTCTCGAATACAAAGATTTTAGAGAGCAAGATACATGAGGCAATGTCGAAAAAGGATACCCTGAAAGCGCGCGCTAGGTCTGCAAAGGTTGCTAGTGAAGTGGGTGAGATGGTGGGGAATGTTGATACGGGTAGTGCCCTTTCGGCTTTTGGAATGATGGAAGAGAAAGTGTTGGCAATGGAAGCTCTTGGCCAGTTAACTATGGATGATGTGGAAGGAAAGTTTGCGGTGCTAGAGAGCTCTTCTTCTGTTGAAGATGACCTTGCAAAGTTGAAGAAACAACTGTCTGCAGGTAGGTCCAAGAAAGGTGAGCTTCCTCCTGGGAGAACCGTTGCTGCTTCAAACAAGGCATACCTGTTAAACGATTCTGTGATTGAGATGGGGCTTAGTGAGTTGAGACTGAAAGCCAAGGTCGACTGCTTGTAA

Protein Analysis

328

Amino Acids

35.74

Weight (kDa)

9.25

Isoelectric Point (pI)

48.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PspA_IM30 PF04012 76 - 280 1.7e-37 PspA/IM30 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 839
Acc65I GGTACC 1 cut(s) 435
AccB1I GGYRCC 1 cut(s) 435
AccI GTMKAC 1 cut(s) 972
AccII CGCG 2 cut(s) 628, 630
AciI CCGC 3 cut(s) 9, 497, 785
AclWI GGATC 1 cut(s) 287
AcoI YGGCCR 3 cut(s) 6, 342, 748
AcuI CTGAAG 1 cut(s) 408
AcvI CACGTG 1 cut(s) 467
AfaI GTAC 1 cut(s) 437
AgeI ACCGGT 2 cut(s) 80, 237
AgsI TTSAA 5 cut(s) 152, 290, 812, 832, 900
AjnI CCWGG 1 cut(s) 877
AluBI AGCT 7 cut(s) 140, 209, 410, 473, 743, 798, 871
AluI AGCT 7 cut(s) 140, 209, 410, 473, 743, 798, 871
Alw21I GWGCWC 2 cut(s) 142, 800
Alw26I GTCTC 4 cut(s) 89, 94, 565, 949
AlwI GGATC 1 cut(s) 287
AoxI GGCC 4 cut(s) 6, 342, 357, 748
ApeKI GCWGC 3 cut(s) 56, 410, 893
AsiGI ACCGGT 2 cut(s) 80, 237
Asp700I GAANNNNTTC 1 cut(s) 230
Asp718I GGTACC 1 cut(s) 435
AspLEI GCGC 3 cut(s) 628, 630, 632
AspS9I GGNCC 1 cut(s) 855
AsuC2I CCSGG 1 cut(s) 130
AsuHPI GGTGA 2 cut(s) 674, 878
AvaII GGWCC 1 cut(s) 855
BaeGI GKGCMC 1 cut(s) 697
BalI TGGCCA 2 cut(s) 344, 750
BanI GGYRCC 1 cut(s) 435
BanII GRGCYC 2 cut(s) 142, 800
BbrPI CACGTG 1 cut(s) 467
BbsI GAAGAC 1 cut(s) 273
Bbv12I GWGCWC 2 cut(s) 142, 800
BbvCI CCTCAGC 1 cut(s) 361
BbvI GCAGC 3 cut(s) 68, 397, 880
BccI CCATC 4 cut(s) 52, 661, 709, 931
BcgI CGANNNNNNTGC 2 cut(s) 224, 258
BciT130I CCWGG 1 cut(s) 879
BciVI GTATCC 1 cut(s) 607
BclI TGATCA 1 cut(s) 529
BcnI CCSGG 1 cut(s) 130
BcoDI GTCTC 4 cut(s) 89, 94, 565, 949
BfaI CTAG 4 cut(s) 507, 633, 651, 791
BfmI CTRYAG 1 cut(s) 846
BfuAI ACCTGC 1 cut(s) 839
BfuI GTATCC 1 cut(s) 607
BglI GCCNNNNNGGC 1 cut(s) 701
BisI GCNGC 4 cut(s) 9, 57, 411, 894
BlsI GCNGC 4 cut(s) 10, 58, 412, 895
Bme1390I CCNGG 2 cut(s) 130, 879
Bme18I GGWCC 1 cut(s) 855
BmgT120I GGNCC 1 cut(s) 855
BmiI GGNNCC 1 cut(s) 437
BmrFI CCNGG 2 cut(s) 130, 879
BmsI GCATC 2 cut(s) 339, 386
BpiI GAAGAC 1 cut(s) 273
Bpu10I CCTNAGC 1 cut(s) 361
BpuEI CTTGAG 1 cut(s) 461
BpuMI CCSGG 1 cut(s) 130
BsaAI YACGTR 1 cut(s) 467
BsaI GGTCTC 2 cut(s) 89, 94
BsaJI CCNNGG 3 cut(s) 129, 878, 966
BsaWI WCCGGW 2 cut(s) 80, 237
Bse118I RCCGGY 2 cut(s) 80, 237
Bse1I ACTGG 1 cut(s) 751
Bse3DI GCAATG 3 cut(s) 378, 606, 741
BseBI CCWGG 1 cut(s) 879
BseDI CCNNGG 3 cut(s) 129, 878, 966
BseGI GGATG 2 cut(s) 71, 769
BseMI GCAATG 3 cut(s) 378, 606, 741
BseMII CTCAG 3 cut(s) 288, 375, 506
BseNI ACTGG 1 cut(s) 751
BsePI GCGCGC 2 cut(s) 626, 628
BseSI GKGCMC 1 cut(s) 697
BseXI GCAGC 3 cut(s) 68, 397, 880
Bsh1236I CGCG 2 cut(s) 628, 630
Bsh1285I CGRYCG 1 cut(s) 238
BshFI GGCC 4 cut(s) 8, 344, 359, 750
BshNI GGYRCC 1 cut(s) 435
BshTI ACCGGT 2 cut(s) 80, 237
BsiEI CGRYCG 1 cut(s) 238
BsiHKAI GWGCWC 2 cut(s) 142, 800
BsiSI CCGG 3 cut(s) 81, 129, 238
BsmAI GTCTC 4 cut(s) 89, 94, 565, 949
BsnI GGCC 4 cut(s) 8, 344, 359, 750
Bso31I GGTCTC 2 cut(s) 89, 94
Bsp1286I GDGCHC 3 cut(s) 142, 697, 800
Bsp143I GATC 4 cut(s) 292, 337, 518, 529
BspACI CCGC 3 cut(s) 9, 497, 785
BspANI GGCC 4 cut(s) 8, 344, 359, 750
BspCNI CTCAG 3 cut(s) 289, 374, 507
BspFNI CGCG 2 cut(s) 628, 630
BspLI GGNNCC 1 cut(s) 437
BspMAI CTGCAG 1 cut(s) 850
BspMI ACCTGC 1 cut(s) 839
BspPI GGATC 1 cut(s) 287
BspQI GCTCTTC 1 cut(s) 805
BspT107I GGYRCC 1 cut(s) 435
BspTNI GGTCTC 2 cut(s) 89, 94
BsrDI GCAATG 3 cut(s) 378, 606, 741
BsrFI RCCGGY 2 cut(s) 80, 237
BsrI ACTGG 1 cut(s) 751
BssAI RCCGGY 2 cut(s) 80, 237
BssECI CCNNGG 3 cut(s) 129, 878, 966
BssHII GCGCGC 2 cut(s) 626, 628
BssMI GATC 4 cut(s) 292, 337, 518, 529
BssT1I CCWWGG 1 cut(s) 966
Bst2UI CCWGG 1 cut(s) 879
Bst4CI ACNGT 2 cut(s) 843, 889
Bst6I CTCTTC 3 cut(s) 67, 714, 805
BstAPI GCANNNNNTGC 1 cut(s) 647
BstBAI YACGTR 1 cut(s) 467
BstC8I GCNNGC 4 cut(s) 54, 315, 628, 630
BstDEI CTNAG 4 cut(s) 297, 361, 515, 944
BstF5I GGATG 2 cut(s) 71, 769
BstFNI CGCG 2 cut(s) 628, 630
BstHHI GCGC 3 cut(s) 628, 630, 632
BstKTI GATC 4 cut(s) 295, 340, 521, 532
BstMAI GTCTC 4 cut(s) 89, 94, 565, 949
BstMBI GATC 4 cut(s) 292, 337, 518, 529
BstMCI CGRYCG 1 cut(s) 238
BstMWI GCNNNNNNNGC 8 cut(s) 215, 419, 470, 503, 638, 647, 701, 740
BstNI CCWGG 1 cut(s) 879
BstSCI CCNGG 2 cut(s) 128, 877
BstSFI CTRYAG 1 cut(s) 846
BstSLI GKGCMC 1 cut(s) 697
BstUI CGCG 2 cut(s) 628, 630
BstV1I GCAGC 3 cut(s) 68, 397, 880
BstV2I GAAGAC 1 cut(s) 273
BstX2I RGATCY 1 cut(s) 292
BstYI RGATCY 1 cut(s) 292
BsuI GTATCC 1 cut(s) 607
BsuRI GGCC 4 cut(s) 8, 344, 359, 750
BtsCI GGATG 2 cut(s) 71, 769
BveI ACCTGC 1 cut(s) 839
Cac8I GCNNGC 4 cut(s) 54, 315, 628, 630
CfoI GCGC 3 cut(s) 628, 630, 632
Cfr10I RCCGGY 2 cut(s) 80, 237
Cfr13I GGNCC 1 cut(s) 855
Csp6I GTAC 1 cut(s) 436
CspAI ACCGGT 2 cut(s) 80, 237
CspCI CAANNNNNGTGG 2 cut(s) 181, 216
CviAII CATG 2 cut(s) 277, 593
CviQI GTAC 1 cut(s) 436
DdeI CTNAG 4 cut(s) 297, 361, 515, 944
DpnI GATC 4 cut(s) 294, 339, 520, 531
DpnII GATC 4 cut(s) 292, 337, 518, 529
EaeI YGGCCR 3 cut(s) 6, 342, 748
Eam1104I CTCTTC 3 cut(s) 67, 714, 805
EarI CTCTTC 3 cut(s) 67, 714, 805
Ecl136II GAGCTC 2 cut(s) 140, 798
Eco130I CCWWGG 1 cut(s) 966
Eco147I AGGCCT 1 cut(s) 359
Eco24I GRGCYC 2 cut(s) 142, 800
Eco31I GGTCTC 2 cut(s) 89, 94
Eco47I GGWCC 1 cut(s) 855
Eco53kI GAGCTC 2 cut(s) 140, 798
Eco57I CTGAAG 1 cut(s) 408
Eco72I CACGTG 1 cut(s) 467
EcoICRI GAGCTC 2 cut(s) 140, 798
EcoRII CCWGG 1 cut(s) 877
EcoT14I CCWWGG 1 cut(s) 966
EcoT38I GRGCYC 2 cut(s) 142, 800
ErhI CCWWGG 1 cut(s) 966
FaeI CATG 2 cut(s) 280, 596
FalI AAGNNNNNCTT 4 cut(s) 804, 836, 855, 887
FatI CATG 2 cut(s) 276, 592
FauNDI CATATG 1 cut(s) 262
FbaI TGATCA 1 cut(s) 529
FblI GTMKAC 1 cut(s) 972
Fnu4HI GCNGC 4 cut(s) 9, 57, 411, 894
FokI GGATG 2 cut(s) 58, 776
FriOI GRGCYC 2 cut(s) 142, 800
Fsp4HI GCNGC 4 cut(s) 9, 57, 411, 894
FspBI CTAG 4 cut(s) 507, 633, 651, 791
GlaI GCGC 3 cut(s) 627, 629, 631
GluI GCNGC 4 cut(s) 9, 57, 411, 894
HaeIII GGCC 4 cut(s) 8, 344, 359, 750
HapII CCGG 3 cut(s) 81, 129, 238
HhaI GCGC 3 cut(s) 628, 630, 632
Hin1II CATG 2 cut(s) 280, 596
Hin6I GCGC 3 cut(s) 626, 628, 630
HinP1I GCGC 3 cut(s) 626, 628, 630
HincII GTYRAC 2 cut(s) 756, 973
HindII GTYRAC 2 cut(s) 756, 973
HinfI GANTC 1 cut(s) 923
HpaI GTTAAC 1 cut(s) 756
HpaII CCGG 3 cut(s) 81, 129, 238
HphI GGTGA 2 cut(s) 674, 878
Hpy166II GTNNAC 2 cut(s) 756, 973
Hpy188I TCNGA 2 cut(s) 427, 516
Hpy188III TCNNGA 5 cut(s) 296, 323, 381, 478, 562
Hpy8I GTNNAC 2 cut(s) 756, 973
Hpy99I CGWCG 1 cut(s) 118
HpyAV CCTTC 3 cut(s) 180, 532, 767
HpyCH4III ACNGT 2 cut(s) 843, 889
HpyCH4IV ACGT 2 cut(s) 113, 466
HpyCH4V TGCA 6 cut(s) 56, 266, 272, 641, 824, 848
HpyF10VI GCNNNNNNNGC 8 cut(s) 215, 419, 470, 503, 638, 647, 701, 740
HpyF3I CTNAG 4 cut(s) 297, 361, 515, 944
HpySE526I ACGT 2 cut(s) 113, 466
Hsp92II CATG 2 cut(s) 280, 596
HspAI GCGC 3 cut(s) 626, 628, 630
KpnI GGTACC 1 cut(s) 439
Ksp22I TGATCA 1 cut(s) 529
KspAI GTTAAC 1 cut(s) 756
Kzo9I GATC 4 cut(s) 292, 337, 518, 529
LguI GCTCTTC 1 cut(s) 805
LmnI GCTCC 2 cut(s) 137, 172
Lsp1109I GCAGC 3 cut(s) 68, 397, 880
LweI GCATC 2 cut(s) 339, 386
MaeI CTAG 4 cut(s) 507, 633, 651, 791
MaeII ACGT 2 cut(s) 113, 466
MalI GATC 4 cut(s) 294, 339, 520, 531
MboI GATC 4 cut(s) 292, 337, 518, 529
MflI RGATCY 1 cut(s) 292
MhlI GDGCHC 3 cut(s) 142, 697, 800
MlsI TGGCCA 2 cut(s) 344, 750
MluCI AATT 1 cut(s) 13
MluNI TGGCCA 2 cut(s) 344, 750
MnlI CCTC 9 cut(s) 70, 85, 114, 370, 421, 445, 474, 589, 885
Mox20I TGGCCA 2 cut(s) 344, 750
MroXI GAANNNNTTC 1 cut(s) 230
MscI TGGCCA 2 cut(s) 344, 750
MseI TTAA 2 cut(s) 755, 917
Msp20I TGGCCA 2 cut(s) 344, 750
MspI CCGG 3 cut(s) 81, 129, 238
MspR9I CCNGG 2 cut(s) 130, 879
MvaI CCWGG 1 cut(s) 879
MvnI CGCG 2 cut(s) 628, 630
MwoI GCNNNNNNNGC 8 cut(s) 215, 419, 470, 503, 638, 647, 701, 740
NciI CCSGG 1 cut(s) 130
NdeI CATATG 1 cut(s) 262
NdeII GATC 4 cut(s) 292, 337, 518, 529
NlaIII CATG 2 cut(s) 280, 596
NlaIV GGNNCC 1 cut(s) 437
PauI GCGCGC 2 cut(s) 626, 628
PceI AGGCCT 1 cut(s) 359
PciSI GCTCTTC 1 cut(s) 805
PdmI GAANNNNTTC 1 cut(s) 230
PfeI GAWTC 1 cut(s) 923
PinAI ACCGGT 2 cut(s) 80, 237
PkrI GCNGC 4 cut(s) 10, 58, 412, 895
PmaCI CACGTG 1 cut(s) 467
PmlI CACGTG 1 cut(s) 467
Ppu21I YACGTR 1 cut(s) 467
Psp124BI GAGCTC 2 cut(s) 142, 800
Psp6I CCWGG 1 cut(s) 877
PspCI CACGTG 1 cut(s) 467
PspGI CCWGG 1 cut(s) 877
PspN4I GGNNCC 1 cut(s) 437
PspPI GGNCC 1 cut(s) 855
PstI CTGCAG 1 cut(s) 850
PsuI RGATCY 1 cut(s) 292
PteI GCGCGC 2 cut(s) 626, 628
RsaI GTAC 1 cut(s) 437
RsaNI GTAC 1 cut(s) 436
SacI GAGCTC 2 cut(s) 142, 800
SalI GTCGAC 1 cut(s) 971
SapI GCTCTTC 1 cut(s) 805
SaqAI TTAA 2 cut(s) 755, 917
SatI GCNGC 4 cut(s) 9, 57, 411, 894
Sau3AI GATC 4 cut(s) 292, 337, 518, 529
Sau96I GGNCC 1 cut(s) 855
ScrFI CCNGG 2 cut(s) 130, 879
SduI GDGCHC 3 cut(s) 142, 697, 800
SfaNI GCATC 2 cut(s) 339, 386
SfcI CTRYAG 1 cut(s) 846
SinI GGWCC 1 cut(s) 855
SmlI CTYRAG 1 cut(s) 476
SmoI CTYRAG 1 cut(s) 476
Sse9I AATT 1 cut(s) 13
SseBI AGGCCT 1 cut(s) 359
SsiI CCGC 3 cut(s) 9, 497, 785
SspMI CTAG 4 cut(s) 507, 633, 651, 791
SstI GAGCTC 2 cut(s) 142, 800
StuI AGGCCT 1 cut(s) 359
StyD4I CCNGG 2 cut(s) 128, 877
StyI CCWWGG 1 cut(s) 966
TaaI ACNGT 2 cut(s) 843, 889
TaiI ACGT 2 cut(s) 116, 469
TaqI TCGA 6 cut(s) 234, 324, 382, 563, 605, 972
TasI AATT 1 cut(s) 13
TauI GCSGC 1 cut(s) 11
TfiI GAWTC 1 cut(s) 923
Tru1I TTAA 2 cut(s) 755, 917
Tru9I TTAA 2 cut(s) 755, 917
TseI GCWGC 3 cut(s) 56, 410, 893
TspDTI ATGAA 5 cut(s) 54, 60, 239, 344, 420
VpaK11BI GGWCC 1 cut(s) 855
XmiI GTMKAC 1 cut(s) 972
XmnI GAANNNNTTC 1 cut(s) 230
XspI CTAG 4 cut(s) 507, 633, 651, 791
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.