FvH4_3g05540

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0003885
Physical Location & Seq
Reverse (-)
3253416 .. 3255488
2073 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g05540.t1

Sequence Viewer

Length: 894 bp
ATGATGGACTCTGCACTTTGTCTCCATCATGGTGCAGCTCTACACATTCCTTCCACCACTGCAGCCGGATTAGCAAGACCCTATTCCAGTTTCAGACCTTCTTCTCTTTTGCTTTTCTCAAAGGCTTCCACATGCCGTCGCACATTATCGCGACATACCTTCAGATGTCCTGTGATATGTGCGCTTAACAAGCCCTCACCACCTTGGCCAAACTACAACAAAGACGATGGTAAAAAAATTATCAGAGTGGTGGGGGGTACTTCTGTAGTTTTGGCTTGTGTCCTTGGAGTCATCCACTGCAACTACATTTTCAGTCGCACAGCCATTGCATACCCTATAGAGCGTCATAATTCAGTTGTCCTGTCTAGGCCCAAAGACGTATTAGACTCGATGTTGAAGGTGACTAGACATTTAGCTACACCCAATATCAAACCGAGCAGGATGTTCATAAGAAGGTTCAGATCGATACCGCAGCTGTCTCTTCCTGATAGACCTGCACCAGAAGACCGTGAAAGATTTAAGATTGAAGTAGCGGATTTAATCAAGTCCGGAATGTGCGATGAGGCGGAGAAACAGCTGATCAATTTATACAAAGAGAAGTTGAAAAAAGGGTCAGAACCTGCGAAGGATGTGGATATGGAATTGGTACTAGTTCTCATTTTCCAGGGAAAATATAAAGAAGCTTTGGCACGCGATTGTCTCCGTTATGAAACCAGTACTTTTTCGGATGGTCGAGTTCATTTTTACAAGGCTATTATCCACACCATGCTAGGCCAGGATGATGATGCTGAGAAGTGCTGGAACTACTTCGTCTCATCCTTTTCCGATACACGGGACATGCTGAAAGCCCTATATGAGGTTCCCAATCCAAAACAACCAAGAACAGAGCGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

298

Amino Acids

33.76

Weight (kDa)

9.41

Isoelectric Point (pI)

48.24

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 502, 628
AccII CGCG 2 cut(s) 151, 693
AccIII TCCGGA 1 cut(s) 548
AciI CCGC 3 cut(s) 470, 533, 566
AcoI YGGCCR 1 cut(s) 206
AcuI CTGAAG 1 cut(s) 145
AfaI GTAC 3 cut(s) 259, 648, 718
AfeI AGCGCT 1 cut(s) 890
AfiI CCNNNNNNNGG 2 cut(s) 831, 856
AgsI TTSAA 3 cut(s) 397, 527, 604
AhlI ACTAGT 1 cut(s) 649
AjnI CCWGG 2 cut(s) 663, 774
AluBI AGCT 5 cut(s) 38, 416, 475, 577, 683
AluI AGCT 5 cut(s) 38, 416, 475, 577, 683
Alw26I GTCTC 4 cut(s) 26, 483, 704, 817
AlwNI CAGNNNCTG 1 cut(s) 620
Aor13HI TCCGGA 1 cut(s) 548
Aor51HI AGCGCT 1 cut(s) 890
AoxI GGCC 3 cut(s) 206, 368, 772
ApeKI GCWGC 3 cut(s) 35, 62, 472
Asp700I GAANNNNTTC 1 cut(s) 806
AspLEI GCGC 2 cut(s) 184, 891
AspS9I GGNCC 1 cut(s) 369
AsuHPI GGTGA 2 cut(s) 189, 412
BaeI ACNNNNGTAYC 2 cut(s) 249, 282
BalI TGGCCA 1 cut(s) 208
BbsI GAAGAC 1 cut(s) 510
BbvI GCAGC 3 cut(s) 47, 74, 484
BccI CCATC 3 cut(s) 33, 221, 722
BceAI ACGGC 1 cut(s) 120
BciT130I CCWGG 2 cut(s) 665, 776
BclI TGATCA 1 cut(s) 579
BcoDI GTCTC 4 cut(s) 26, 483, 704, 817
BcuI ACTAGT 1 cut(s) 649
BfaI CTAG 4 cut(s) 366, 405, 650, 770
BfmI CTRYAG 3 cut(s) 60, 264, 336
BfoI RGCGCY 1 cut(s) 892
BfuAI ACCTGC 2 cut(s) 502, 628
BisI GCNGC 3 cut(s) 36, 63, 473
BlsI GCNGC 3 cut(s) 37, 64, 474
BmcAI AGTACT 1 cut(s) 718
Bme1390I CCNGG 2 cut(s) 665, 776
BmgT120I GGNCC 1 cut(s) 369
BmiI GGNNCC 1 cut(s) 861
BmrFI CCNGG 2 cut(s) 665, 776
BmsI GCATC 1 cut(s) 775
BpiI GAAGAC 1 cut(s) 510
Bsa29I ATCGAT 1 cut(s) 464
BsaJI CCNNGG 3 cut(s) 203, 283, 664
BsaWI WCCGGW 1 cut(s) 548
BsaXI ACNNNNNCTCC 2 cut(s) 6, 36
Bsc4I CCNNNNNNNGG 2 cut(s) 831, 856
Bse1I ACTGG 2 cut(s) 87, 714
Bse3DI GCAATG 1 cut(s) 324
BseAI TCCGGA 1 cut(s) 548
BseBI CCWGG 2 cut(s) 665, 776
BseCI ATCGAT 1 cut(s) 464
BseDI CCNNGG 3 cut(s) 203, 283, 664
BseGI GGATG 6 cut(s) 291, 447, 634, 733, 784, 815
BseLI CCNNNNNNNGG 2 cut(s) 831, 856
BseMI GCAATG 1 cut(s) 324
BseMII CTCAG 1 cut(s) 780
BseNI ACTGG 2 cut(s) 87, 714
BseXI GCAGC 3 cut(s) 47, 74, 484
BsgI GTGCAG 2 cut(s) 54, 480
Bsh1236I CGCG 2 cut(s) 151, 693
BshFI GGCC 3 cut(s) 208, 370, 774
BshVI ATCGAT 1 cut(s) 464
BsiSI CCGG 2 cut(s) 66, 549
BslFI GGGAC 1 cut(s) 848
BslI CCNNNNNNNGG 2 cut(s) 831, 856
BsmAI GTCTC 4 cut(s) 26, 483, 704, 817
BsmBI CGTCTC 1 cut(s) 817
BsmFI GGGAC 1 cut(s) 848
BsnI GGCC 3 cut(s) 208, 370, 774
Bsp13I TCCGGA 1 cut(s) 548
Bsp143I GATC 2 cut(s) 461, 579
Bsp68I TCGCGA 1 cut(s) 151
BspACI CCGC 3 cut(s) 470, 533, 566
BspANI GGCC 3 cut(s) 208, 370, 774
BspCNI CTCAG 1 cut(s) 781
BspDI ATCGAT 1 cut(s) 464
BspEI TCCGGA 1 cut(s) 548
BspFNI CGCG 2 cut(s) 151, 693
BspLI GGNNCC 1 cut(s) 861
BspMAI CTGCAG 1 cut(s) 64
BspMI ACCTGC 2 cut(s) 502, 628
BsrDI GCAATG 1 cut(s) 324
BsrI ACTGG 2 cut(s) 87, 714
BssECI CCNNGG 3 cut(s) 203, 283, 664
BssMI GATC 2 cut(s) 461, 579
BssT1I CCWWGG 2 cut(s) 203, 283
Bst2UI CCWGG 2 cut(s) 665, 776
Bst4CI ACNGT 1 cut(s) 509
Bst6I CTCTTC 1 cut(s) 486
BstC8I GCNNGC 1 cut(s) 691
BstDEI CTNAG 1 cut(s) 789
BstENI CCTNNNNNAGG 1 cut(s) 854
BstF5I GGATG 6 cut(s) 291, 447, 634, 733, 784, 815
BstFNI CGCG 2 cut(s) 151, 693
BstH2I RGCGCY 1 cut(s) 892
BstHHI GCGC 2 cut(s) 184, 891
BstKTI GATC 2 cut(s) 464, 582
BstMAI GTCTC 4 cut(s) 26, 483, 704, 817
BstMBI GATC 2 cut(s) 461, 579
BstMWI GCNNNNNNNGC 2 cut(s) 71, 190
BstNI CCWGG 2 cut(s) 665, 776
BstNSI RCATGY 2 cut(s) 135, 841
BstSCI CCNGG 2 cut(s) 663, 774
BstSFI CTRYAG 3 cut(s) 60, 264, 336
BstUI CGCG 2 cut(s) 151, 693
BstV1I GCAGC 3 cut(s) 47, 74, 484
BstV2I GAAGAC 1 cut(s) 510
Bsu15I ATCGAT 1 cut(s) 464
BsuRI GGCC 3 cut(s) 208, 370, 774
BsuTUI ATCGAT 1 cut(s) 464
BtgZI GCGATG 1 cut(s) 573
BtsCI GGATG 6 cut(s) 291, 447, 634, 733, 784, 815
BtsI GCAGTG 2 cut(s) 57, 295
BtsIMutI CAGTG 2 cut(s) 57, 295
BtuMI TCGCGA 1 cut(s) 151
BveI ACCTGC 2 cut(s) 502, 628
Cac8I GCNNGC 1 cut(s) 691
CaiI CAGNNNCTG 1 cut(s) 620
CfoI GCGC 2 cut(s) 184, 891
Cfr13I GGNCC 1 cut(s) 369
ClaI ATCGAT 1 cut(s) 464
CseI GACGC 1 cut(s) 332
Csp6I GTAC 3 cut(s) 258, 647, 717
CviAII CATG 4 cut(s) 29, 132, 766, 838
CviQI GTAC 3 cut(s) 258, 647, 717
DdeI CTNAG 1 cut(s) 789
DpnI GATC 2 cut(s) 463, 581
DpnII GATC 2 cut(s) 461, 579
EaeI YGGCCR 1 cut(s) 206
Eam1104I CTCTTC 1 cut(s) 486
EarI CTCTTC 1 cut(s) 486
EciI GGCGGA 1 cut(s) 581
Eco130I CCWWGG 2 cut(s) 203, 283
Eco47III AGCGCT 1 cut(s) 890
Eco57I CTGAAG 1 cut(s) 145
EcoNI CCTNNNNNAGG 1 cut(s) 854
EcoRII CCWGG 2 cut(s) 663, 774
EcoT14I CCWWGG 2 cut(s) 203, 283
ErhI CCWWGG 2 cut(s) 203, 283
Esp3I CGTCTC 1 cut(s) 817
FaeI CATG 4 cut(s) 32, 135, 769, 841
FaqI GGGAC 1 cut(s) 848
FatI CATG 4 cut(s) 28, 131, 765, 837
FbaI TGATCA 1 cut(s) 579
Fnu4HI GCNGC 3 cut(s) 36, 63, 473
FokI GGATG 6 cut(s) 278, 454, 641, 740, 791, 802
Fsp4HI GCNGC 3 cut(s) 36, 63, 473
FspBI CTAG 4 cut(s) 366, 405, 650, 770
GlaI GCGC 2 cut(s) 183, 890
GluI GCNGC 3 cut(s) 36, 63, 473
HaeII RGCGCY 1 cut(s) 892
HaeIII GGCC 3 cut(s) 208, 370, 774
HapII CCGG 2 cut(s) 66, 549
HgaI GACGC 1 cut(s) 332
HhaI GCGC 2 cut(s) 184, 891
Hin1II CATG 4 cut(s) 32, 135, 769, 841
Hin6I GCGC 2 cut(s) 182, 889
HinP1I GCGC 2 cut(s) 182, 889
HindIII AAGCTT 1 cut(s) 681
HinfI GANTC 3 cut(s) 8, 288, 386
HpaII CCGG 2 cut(s) 66, 549
HphI GGTGA 2 cut(s) 189, 412
Hpy188I TCNGA 7 cut(s) 95, 164, 245, 461, 616, 727, 826
Hpy188III TCNNGA 3 cut(s) 150, 485, 549
Hpy99I CGWCG 1 cut(s) 141
HpyAV CCTTC 6 cut(s) 60, 108, 169, 391, 447, 619
HpyCH4III ACNGT 1 cut(s) 509
HpyCH4IV ACGT 1 cut(s) 378
HpyCH4V TGCA 6 cut(s) 14, 35, 62, 300, 329, 497
HpyF10VI GCNNNNNNNGC 2 cut(s) 71, 190
HpyF3I CTNAG 1 cut(s) 789
HpySE526I ACGT 1 cut(s) 378
Hsp92II CATG 4 cut(s) 32, 135, 769, 841
HspAI GCGC 2 cut(s) 182, 889
Kpn2I TCCGGA 1 cut(s) 548
Ksp22I TGATCA 1 cut(s) 579
Kzo9I GATC 2 cut(s) 461, 579
Lsp1109I GCAGC 3 cut(s) 47, 74, 484
LweI GCATC 1 cut(s) 775
MaeI CTAG 4 cut(s) 366, 405, 650, 770
MaeII ACGT 1 cut(s) 378
MaeIII GTNAC 1 cut(s) 400
MalI GATC 2 cut(s) 463, 581
MboI GATC 2 cut(s) 461, 579
MboII GAAGA 3 cut(s) 93, 473, 515
MlsI TGGCCA 1 cut(s) 208
MluCI AATT 4 cut(s) 237, 349, 583, 641
MluNI TGGCCA 1 cut(s) 208
MlyI GAGTC 3 cut(s) 2, 297, 380
MnlI CCTC 3 cut(s) 205, 556, 850
Mox20I TGGCCA 1 cut(s) 208
MroI TCCGGA 1 cut(s) 548
MroXI GAANNNNTTC 1 cut(s) 806
MscI TGGCCA 1 cut(s) 208
MseI TTAA 3 cut(s) 186, 519, 539
MslI CAYNNNNRTG 1 cut(s) 30
Msp20I TGGCCA 1 cut(s) 208
MspA1I CMGCKG 2 cut(s) 475, 577
MspI CCGG 2 cut(s) 66, 549
MspR9I CCNGG 2 cut(s) 665, 776
MvaI CCWGG 2 cut(s) 665, 776
MvnI CGCG 2 cut(s) 151, 693
MwoI GCNNNNNNNGC 2 cut(s) 71, 190
NdeII GATC 2 cut(s) 461, 579
NlaIII CATG 4 cut(s) 32, 135, 769, 841
NlaIV GGNNCC 1 cut(s) 861
NmuCI GTSAC 1 cut(s) 400
NruI TCGCGA 1 cut(s) 151
NspI RCATGY 2 cut(s) 135, 841
PdmI GAANNNNTTC 1 cut(s) 806
PkrI GCNGC 3 cut(s) 37, 64, 474
PleI GAGTC 3 cut(s) 2, 296, 380
PpsI GAGTC 3 cut(s) 2, 296, 380
Psp6I CCWGG 2 cut(s) 663, 774
PspGI CCWGG 2 cut(s) 663, 774
PspN4I GGNNCC 1 cut(s) 861
PspPI GGNCC 1 cut(s) 369
PstI CTGCAG 1 cut(s) 64
PstNI CAGNNNCTG 1 cut(s) 620
PvuII CAGCTG 2 cut(s) 475, 577
RruI TCGCGA 1 cut(s) 151
RsaI GTAC 3 cut(s) 259, 648, 718
RsaNI GTAC 3 cut(s) 258, 647, 717
RseI CAYNNNNRTG 1 cut(s) 30
SaqAI TTAA 3 cut(s) 186, 519, 539
SatI GCNGC 3 cut(s) 36, 63, 473
Sau3AI GATC 2 cut(s) 461, 579
Sau96I GGNCC 1 cut(s) 369
ScaI AGTACT 1 cut(s) 718
SchI GAGTC 3 cut(s) 2, 297, 380
ScrFI CCNGG 2 cut(s) 665, 776
SfaNI GCATC 1 cut(s) 775
SfcI CTRYAG 3 cut(s) 60, 264, 336
SmiMI CAYNNNNRTG 1 cut(s) 30
SpeI ACTAGT 1 cut(s) 649
Sse9I AATT 4 cut(s) 237, 349, 583, 641
SsiI CCGC 3 cut(s) 470, 533, 566
SspMI CTAG 4 cut(s) 366, 405, 650, 770
StyD4I CCNGG 2 cut(s) 663, 774
StyI CCWWGG 2 cut(s) 203, 283
TaaI ACNGT 1 cut(s) 509
TaiI ACGT 1 cut(s) 381
TaqI TCGA 3 cut(s) 389, 464, 733
TasI AATT 4 cut(s) 237, 349, 583, 641
TatI WGTACW 1 cut(s) 716
Tru1I TTAA 3 cut(s) 186, 519, 539
Tru9I TTAA 3 cut(s) 186, 519, 539
TscAI CASTG 2 cut(s) 64, 302
TseFI GTSAC 1 cut(s) 400
TseI GCWGC 3 cut(s) 35, 62, 472
Tsp45I GTSAC 1 cut(s) 400
TspDTI ATGAA 3 cut(s) 436, 723, 728
TspGWI ACGGA 1 cut(s) 692
TspRI CASTG 2 cut(s) 64, 302
XagI CCTNNNNNAGG 1 cut(s) 854
XceI RCATGY 2 cut(s) 135, 841
XmnI GAANNNNTTC 1 cut(s) 806
XspI CTAG 4 cut(s) 366, 405, 650, 770
ZrmI AGTACT 1 cut(s) 718
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.