Rorug05G0015800

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0003885
Physical Location & Seq
Forward (+)
1063483 .. 1066037
2555 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0015800.1

Sequence Viewer

Length: 1014 bp
ATGGCAGATGGCTCCAGATTCAAACCCTACAGTCGCAACAGTACTCAAAGAAGCCGAACCAGCACCGACTCCAGCTCCCCGGAATTCGACTTCTGGAGGCTGCAAAACCCGTCTTGCCCGCAACCCGATCTCATATCTGCCGATGACCTATTCGTCGACGGTGTCATTCTCCCTCTCAGCCTCGTCCCCGCCCCCAAAAACCAGCCCGACCCGAACCCGCGACCCGACTCGGAAGAGATTTCTGCTCCGGACCCAGAACCGGAACCCGGCCCGGAAGCCCAGGTGTCGACCGGAGCTCTGGTGCTGACCGCATCGAAGCGGTGGAGGGACATGTTCAAGAAGAAGAGTGAGGGTAGTGAGAAGGAGAAGGAGAAGGAGAAGAAGAAGGAGCGGAAGAGCGGCGGAGCAGCCACCGGAGCGAGCTCGGCGGAGCTGAACATCAATCTGTGGCCGTTTTCGCGGAGCAGATCCGCCGGAAATGCTTGTACCCGACCCAAATTCGGGGCTCCGGGAACCCGGAAGGTGAACAGCGCGCCGTGTTCGAGGAGCAACTCGACGGGCGAGTCCAAGTCAACGAGGAAGTGGCCGGCGAGTCCGGGTCGGGCCGGAGTCCACTTGGGTCGGAGCAGCCCGGTTTGGCAGGTCCGGCGTGGAGCCTCAACCCTGGGTAAAACCTCCGAAACCCAAGTCAGGAATGCTGAAAAGAGCACCAAGAAAGAAGCACCCGAAACTCGCCGGAGTAAAACCACCGCATCCGGCGACGGTCGCGGCGGAGGAGGTGGTGCAAAACCCAGAGTCTTGAACTTGAATGTTCCGACGTGTATCGGGTACAGAAGCCATTTGAGTTGTAGAAGTGACGTCATGAATAGTGCTGTAGGAATCGGCGGCAGTAGTGGTAGCAACAAACACCGTGGGGCCGCCGCCGCTGGCGGTGGCGGAGATAGTAGTAACGCTGCTGGGGCTGGTAGTAATCTTTTTAATCTGCGCAGCCTTTTCTCTAAGAAGGTGTATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

337

Amino Acids

35.66

Weight (kDa)

10.09

Isoelectric Point (pI)

60.93

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 152, 562
AatII GACGTC 1 cut(s) 861
Acc16I TGCGCA 1 cut(s) 986
Acc36I ACCTGC 1 cut(s) 631
AccBSI CCGCTC 2 cut(s) 391, 399
AccI GTMKAC 2 cut(s) 156, 287
AccII CGCG 4 cut(s) 220, 460, 533, 768
AccIII TCCGGA 1 cut(s) 247
AclWI GGATC 1 cut(s) 462
AcoI YGGCCR 2 cut(s) 449, 584
AcsI RAATTY 2 cut(s) 83, 497
AcyI GRCGYC 1 cut(s) 858
AfaI GTAC 3 cut(s) 43, 487, 830
AfiI CCNNNNNNNGG 5 cut(s) 259, 266, 500, 501, 690
AflIII ACRYGT 2 cut(s) 330, 818
AgsI TTSAA 4 cut(s) 22, 337, 802, 808
AjiI CACGTC 1 cut(s) 819
AjnI CCWGG 2 cut(s) 279, 663
AluBI AGCT 4 cut(s) 75, 296, 423, 433
AluI AGCT 4 cut(s) 75, 296, 423, 433
Alw21I GWGCWC 3 cut(s) 298, 425, 710
AlwI GGATC 1 cut(s) 462
Aor13HI TCCGGA 1 cut(s) 247
AoxI GGCC 5 cut(s) 268, 449, 584, 603, 915
ApeKI GCWGC 5 cut(s) 100, 407, 627, 953, 987
ApoI RAATTY 2 cut(s) 83, 497
ArsI GACNNNNNNTTYG 2 cut(s) 672, 704
AspLEI GCGC 3 cut(s) 533, 535, 987
AspS9I GGNCC 5 cut(s) 250, 269, 603, 643, 915
AsuC2I CCSGG 7 cut(s) 80, 267, 272, 510, 517, 597, 632
AsuHPI GGTGA 1 cut(s) 535
AvaII GGWCC 2 cut(s) 250, 643
BanII GRGCYC 3 cut(s) 298, 425, 508
Bbv12I GWGCWC 3 cut(s) 298, 425, 710
BbvI GCAGC 5 cut(s) 87, 419, 639, 940, 999
BccI CCATC 1 cut(s) 2
BceAI ACGGC 2 cut(s) 436, 520
BciT130I CCWGG 2 cut(s) 281, 665
BcnI CCSGG 7 cut(s) 80, 267, 272, 510, 517, 597, 632
BfmI CTRYAG 2 cut(s) 28, 873
BfuAI ACCTGC 1 cut(s) 631
BmcAI AGTACT 1 cut(s) 43
Bme1390I CCNGG 9 cut(s) 80, 267, 272, 281, 510, 517, 597, 632, 665
Bme18I GGWCC 2 cut(s) 250, 643
BmgBI CACGTC 1 cut(s) 819
BmgT120I GGNCC 5 cut(s) 250, 269, 603, 643, 915
BmiI GGNNCC 7 cut(s) 13, 252, 264, 507, 514, 655, 916
BmrFI CCNGG 9 cut(s) 80, 267, 272, 281, 510, 517, 597, 632, 665
BmsI GCATC 2 cut(s) 320, 761
BpmI CTGGAG 2 cut(s) 55, 115
BpuMI CCSGG 7 cut(s) 80, 267, 272, 510, 517, 597, 632
BsaHI GRCGYC 1 cut(s) 858
BsaJI CCNNGG 5 cut(s) 78, 279, 663, 664, 910
BsaWI WCCGGW 4 cut(s) 247, 259, 290, 413
BsaXI ACNNNNNCTCC 4 cut(s) 59, 89, 285, 315
Bsc4I CCNNNNNNNGG 5 cut(s) 259, 266, 500, 501, 690
Bse118I RCCGGY 1 cut(s) 586
BseAI TCCGGA 1 cut(s) 247
BseBI CCWGG 2 cut(s) 281, 665
BseDI CCNNGG 5 cut(s) 78, 279, 663, 664, 910
BseGI GGATG 1 cut(s) 752
BseLI CCNNNNNNNGG 5 cut(s) 259, 266, 500, 501, 690
BseMII CTCAG 1 cut(s) 190
BsePI GCGCGC 1 cut(s) 531
BseRI GAGGAG 2 cut(s) 559, 789
BseXI GCAGC 5 cut(s) 87, 419, 639, 940, 999
BseYI CCCAGC 1 cut(s) 956
Bsh1236I CGCG 4 cut(s) 220, 460, 533, 768
Bsh1285I CGRYCG 2 cut(s) 291, 766
BshFI GGCC 5 cut(s) 270, 451, 586, 605, 917
BsiEI CGRYCG 2 cut(s) 291, 766
BsiHKAI GWGCWC 3 cut(s) 298, 425, 710
BslFI GGGAC 2 cut(s) 170, 341
BslI CCNNNNNNNGG 5 cut(s) 259, 266, 500, 501, 690
BsmFI GGGAC 2 cut(s) 170, 341
BsmI GAATGC 1 cut(s) 700
BsnI GGCC 5 cut(s) 270, 451, 586, 605, 917
Bsp1286I GDGCHC 4 cut(s) 298, 425, 508, 710
Bsp13I TCCGGA 1 cut(s) 247
Bsp143I GATC 2 cut(s) 127, 467
BspANI GGCC 5 cut(s) 270, 451, 586, 605, 917
BspCNI CTCAG 1 cut(s) 189
BspEI TCCGGA 1 cut(s) 247
BspFNI CGCG 4 cut(s) 220, 460, 533, 768
BspHI TCATGA 1 cut(s) 861
BspLI GGNNCC 7 cut(s) 13, 252, 264, 507, 514, 655, 916
BspMI ACCTGC 1 cut(s) 631
BspPI GGATC 1 cut(s) 462
BspQI GCTCTTC 1 cut(s) 389
BsrBI CCGCTC 2 cut(s) 391, 399
BsrFI RCCGGY 1 cut(s) 586
BssAI RCCGGY 1 cut(s) 586
BssECI CCNNGG 5 cut(s) 78, 279, 663, 664, 910
BssHII GCGCGC 1 cut(s) 531
BssMI GATC 2 cut(s) 127, 467
BssNI GRCGYC 1 cut(s) 858
Bst2UI CCWGG 2 cut(s) 281, 665
Bst4CI ACNGT 5 cut(s) 32, 41, 161, 764, 911
Bst6I CTCTTC 3 cut(s) 228, 338, 389
BstACI GRCGYC 1 cut(s) 858
BstC8I GCNNGC 5 cut(s) 119, 421, 533, 588, 928
BstDEI CTNAG 2 cut(s) 176, 999
BstDSI CCRYGG 1 cut(s) 910
BstF5I GGATG 1 cut(s) 752
BstFNI CGCG 4 cut(s) 220, 460, 533, 768
BstHHI GCGC 3 cut(s) 533, 535, 987
BstKTI GATC 2 cut(s) 130, 470
BstMBI GATC 2 cut(s) 127, 467
BstMCI CGRYCG 2 cut(s) 291, 766
BstMWI GCNNNNNNNGC 9 cut(s) 60, 416, 425, 457, 479, 646, 765, 923, 959
BstNI CCWGG 2 cut(s) 281, 665
BstNSI RCATGY 1 cut(s) 334
BstSCI CCNGG 9 cut(s) 78, 265, 270, 279, 508, 515, 595, 630, 663
BstSFI CTRYAG 2 cut(s) 28, 873
BstUI CGCG 4 cut(s) 220, 460, 533, 768
BstV1I GCAGC 5 cut(s) 87, 419, 639, 940, 999
BstX2I RGATCY 1 cut(s) 467
BstYI RGATCY 1 cut(s) 467
BsuRI GGCC 5 cut(s) 270, 451, 586, 605, 917
BtgI CCRYGG 1 cut(s) 910
BtrI CACGTC 1 cut(s) 819
BtsCI GGATG 1 cut(s) 752
BveI ACCTGC 1 cut(s) 631
Cac8I GCNNGC 5 cut(s) 119, 421, 533, 588, 928
CciI TCATGA 1 cut(s) 861
CfoI GCGC 3 cut(s) 533, 535, 987
Cfr10I RCCGGY 1 cut(s) 586
Cfr13I GGNCC 5 cut(s) 250, 269, 603, 643, 915
Csp6I GTAC 3 cut(s) 42, 486, 829
CspCI CAANNNNNGTGG 2 cut(s) 892, 927
CviAII CATG 2 cut(s) 331, 862
CviQI GTAC 3 cut(s) 42, 486, 829
DdeI CTNAG 2 cut(s) 176, 999
DpnI GATC 2 cut(s) 129, 469
DpnII GATC 2 cut(s) 127, 467
DrdI GACNNNNNNGTC 2 cut(s) 152, 562
DseDI GACNNNNNNGTC 2 cut(s) 152, 562
EaeI YGGCCR 2 cut(s) 449, 584
Eam1104I CTCTTC 3 cut(s) 228, 338, 389
EarI CTCTTC 3 cut(s) 228, 338, 389
EciI GGCGGA 5 cut(s) 417, 443, 460, 786, 951
Ecl136II GAGCTC 2 cut(s) 296, 423
Eco24I GRGCYC 3 cut(s) 298, 425, 508
Eco47I GGWCC 2 cut(s) 250, 643
Eco53kI GAGCTC 2 cut(s) 296, 423
EcoICRI GAGCTC 2 cut(s) 296, 423
EcoRI GAATTC 1 cut(s) 83
EcoRII CCWGG 2 cut(s) 279, 663
EcoT38I GRGCYC 3 cut(s) 298, 425, 508
FaeI CATG 2 cut(s) 334, 865
FaiI YATR 3 cut(s) 134, 332, 863
FaqI GGGAC 2 cut(s) 170, 341
FatI CATG 2 cut(s) 330, 861
FauI CCCGC 3 cut(s) 126, 196, 225
FblI GTMKAC 2 cut(s) 156, 287
FokI GGATG 1 cut(s) 739
FriOI GRGCYC 3 cut(s) 298, 425, 508
FspI TGCGCA 1 cut(s) 986
GlaI GCGC 3 cut(s) 532, 534, 986
GsaI CCCAGC 1 cut(s) 960
GsuI CTGGAG 2 cut(s) 55, 115
HaeIII GGCC 5 cut(s) 270, 451, 586, 605, 917
HhaI GCGC 3 cut(s) 533, 535, 987
Hin1I GRCGYC 1 cut(s) 858
Hin1II CATG 2 cut(s) 334, 865
Hin6I GCGC 3 cut(s) 531, 533, 985
HinP1I GCGC 3 cut(s) 531, 533, 985
HincII GTYRAC 3 cut(s) 157, 288, 573
HindII GTYRAC 3 cut(s) 157, 288, 573
HinfI GANTC 8 cut(s) 18, 68, 227, 563, 592, 609, 795, 879
HphI GGTGA 1 cut(s) 535
Hpy166II GTNNAC 5 cut(s) 157, 288, 526, 573, 613
Hpy188I TCNGA 4 cut(s) 232, 624, 679, 816
Hpy188III TCNNGA 7 cut(s) 15, 94, 248, 337, 691, 799, 862
Hpy8I GTNNAC 5 cut(s) 157, 288, 526, 573, 613
Hpy99I CGWCG 5 cut(s) 158, 161, 559, 764, 820
HpyAV CCTTC 6 cut(s) 355, 361, 367, 379, 514, 997
HpyCH4III ACNGT 5 cut(s) 32, 41, 161, 764, 911
HpyCH4IV ACGT 2 cut(s) 818, 858
HpyCH4V TGCA 2 cut(s) 103, 785
HpyF10VI GCNNNNNNNGC 9 cut(s) 60, 416, 425, 457, 479, 646, 765, 923, 959
HpyF3I CTNAG 2 cut(s) 176, 999
HpySE526I ACGT 2 cut(s) 818, 858
Hsp92I GRCGYC 1 cut(s) 858
Hsp92II CATG 2 cut(s) 334, 865
HspAI GCGC 3 cut(s) 531, 533, 985
Kpn2I TCCGGA 1 cut(s) 247
KroI GCCGGC 1 cut(s) 586
KroNI GCCGGC 1 cut(s) 588
Kzo9I GATC 2 cut(s) 127, 467
LguI GCTCTTC 1 cut(s) 389
Lsp1109I GCAGC 5 cut(s) 87, 419, 639, 940, 999
LweI GCATC 2 cut(s) 320, 761
MaeII ACGT 2 cut(s) 818, 858
MaeIII GTNAC 2 cut(s) 854, 947
MalI GATC 2 cut(s) 129, 469
MbiI CCGCTC 2 cut(s) 391, 399
MboI GATC 2 cut(s) 127, 467
MboII GAAGA 6 cut(s) 245, 352, 355, 391, 394, 406
MflI RGATCY 1 cut(s) 467
MhlI GDGCHC 4 cut(s) 298, 425, 508, 710
MluCI AATT 2 cut(s) 83, 497
MlyI GAGTC 6 cut(s) 62, 221, 572, 601, 618, 804
MmeI TCCRAC 2 cut(s) 602, 839
MroI TCCGGA 1 cut(s) 247
MroNI GCCGGC 1 cut(s) 586
MseI TTAA 2 cut(s) 978, 1012
MspA1I CMGCKG 1 cut(s) 926
MspR9I CCNGG 9 cut(s) 80, 267, 272, 281, 510, 517, 597, 632, 665
Mva1269I GAATGC 1 cut(s) 700
MvaI CCWGG 2 cut(s) 281, 665
MvnI CGCG 4 cut(s) 220, 460, 533, 768
MwoI GCNNNNNNNGC 9 cut(s) 60, 416, 425, 457, 479, 646, 765, 923, 959
NaeI GCCGGC 1 cut(s) 588
NciI CCSGG 7 cut(s) 80, 267, 272, 510, 517, 597, 632
NdeII GATC 2 cut(s) 127, 467
NgoMIV GCCGGC 1 cut(s) 586
NlaIII CATG 2 cut(s) 334, 865
NlaIV GGNNCC 7 cut(s) 13, 252, 264, 507, 514, 655, 916
NmeAIII GCCGAG 1 cut(s) 404
NmuCI GTSAC 1 cut(s) 854
NsbI TGCGCA 1 cut(s) 986
NspI RCATGY 1 cut(s) 334
PagI TCATGA 1 cut(s) 861
PasI CCCWGGG 1 cut(s) 664
PauI GCGCGC 1 cut(s) 531
PciI ACATGT 1 cut(s) 330
PciSI GCTCTTC 1 cut(s) 389
PctI GAATGC 1 cut(s) 700
PdiI GCCGGC 1 cut(s) 588
PfeI GAWTC 2 cut(s) 18, 879
PflFI GACNNNGTC 1 cut(s) 161
PfoI TCCNGGA 1 cut(s) 508
PleI GAGTC 6 cut(s) 62, 221, 571, 600, 617, 803
PpsI GAGTC 6 cut(s) 62, 221, 571, 600, 617, 803
PscI ACATGT 1 cut(s) 330
Psp124BI GAGCTC 2 cut(s) 298, 425
Psp6I CCWGG 2 cut(s) 279, 663
PspFI CCCAGC 1 cut(s) 956
PspGI CCWGG 2 cut(s) 279, 663
PspN4I GGNNCC 7 cut(s) 13, 252, 264, 507, 514, 655, 916
PspPI GGNCC 5 cut(s) 250, 269, 603, 643, 915
PsuI RGATCY 1 cut(s) 467
PsyI GACNNNGTC 1 cut(s) 161
PteI GCGCGC 1 cut(s) 531
RsaI GTAC 3 cut(s) 43, 487, 830
RsaNI GTAC 3 cut(s) 42, 486, 829
SacI GAGCTC 2 cut(s) 298, 425
SalI GTCGAC 2 cut(s) 155, 286
SapI GCTCTTC 1 cut(s) 389
SaqAI TTAA 2 cut(s) 978, 1012
Sau3AI GATC 2 cut(s) 127, 467
Sau96I GGNCC 5 cut(s) 250, 269, 603, 643, 915
ScaI AGTACT 1 cut(s) 43
SchI GAGTC 6 cut(s) 62, 221, 572, 601, 618, 804
ScrFI CCNGG 9 cut(s) 80, 267, 272, 281, 510, 517, 597, 632, 665
SduI GDGCHC 4 cut(s) 298, 425, 508, 710
SfaNI GCATC 2 cut(s) 320, 761
SfcI CTRYAG 2 cut(s) 28, 873
SgrDI CGTCGACG 1 cut(s) 155
SinI GGWCC 2 cut(s) 250, 643
Sse9I AATT 2 cut(s) 83, 497
SstI GAGCTC 2 cut(s) 298, 425
StyD4I CCNGG 9 cut(s) 78, 265, 270, 279, 508, 515, 595, 630, 663
TaaI ACNGT 5 cut(s) 32, 41, 161, 764, 911
TaiI ACGT 2 cut(s) 821, 861
TaqI TCGA 6 cut(s) 87, 156, 287, 314, 542, 554
TasI AATT 2 cut(s) 83, 497
TatI WGTACW 1 cut(s) 41
TauI GCSGC 6 cut(s) 402, 771, 888, 920, 923, 926
TfiI GAWTC 2 cut(s) 18, 879
Tru1I TTAA 2 cut(s) 978, 1012
Tru9I TTAA 2 cut(s) 978, 1012
TseFI GTSAC 1 cut(s) 854
TseI GCWGC 5 cut(s) 100, 407, 627, 953, 987
Tsp45I GTSAC 1 cut(s) 854
TspDTI ATGAA 1 cut(s) 878
Tth111I GACNNNGTC 1 cut(s) 161
VpaK11BI GGWCC 2 cut(s) 250, 643
XapI RAATTY 2 cut(s) 83, 497
XceI RCATGY 1 cut(s) 334
XmiI GTMKAC 2 cut(s) 156, 287
ZraI GACGTC 1 cut(s) 859
ZrmI AGTACT 1 cut(s) 43
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.