FvH4_3g06670

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0001843
Physical Location & Seq
Forward (+)
3838516 .. 3839912
1397 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g06670.t1

Sequence Viewer

Length: 717 bp
ATGGGTAAACAGTTAAAGAACCACAGGAAAACTATCTCGCGCATCATCGGCATGCTTGGAAAGAAAAGCTTGGCCAGAAACTACTTGAACAAGTGCTTATATTCAGTCGCAACAGGCAGCAATGACTACATTAACAATTACTTTTTACCTCAATACTATAATACTAGTCAAGAATATACACTTGAGGAATATGCCGTAGTTCTTGTCGAACAATATTCTCGGCAAATACTGAGATTGTATGAGTATGGAGCAAAGAAGGTTGTTTTGGGTGGACTGGGACTAATAGGCTGCACTCCTGCTGCAATTGCTAGCTATGGCACCAACGGATCATGTTCTGAAGAACTCAACTATGCAGCCCAACTTTTCAACCAACAGCTTGTATCTCTTGTTGATCAGCTCAACAGCAATTTGACTGATGCAAAGTTCATCTACATTAACAACTACGGAATCGGTTCGGTCAATACTACAAGTCTTGGATTCACGGTGTCGGATGCTGGGTGCTGTACACTTAACAGCGGTGGCCTGTGTGTGGAAGATTCAACTCCATGCGAGGATAGGAGTGAGTCTGTGTTTTGGGACGGGTTCCATCCTACTGAGGCTTTTAATCGGATCAGTGCTAACAAAAGCTACAGTTCGTATGAGTCTGCCGACACTTATCCAATGGATGTTAGTCAACTAGTTGAGCTAGAGTTCAATCCTCTGGTGGCAGAGATGTAG

Protein Analysis

239

Amino Acids

26.38

Weight (kDa)

4.89

Isoelectric Point (pI)

33.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 21 - 207 2.4e-22 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 317
AccII CGCG 1 cut(s) 40
AciI CCGC 1 cut(s) 516
AclWI GGATC 2 cut(s) 334, 617
AcoI YGGCCR 1 cut(s) 72
AcuI CTGAAG 1 cut(s) 357
AfaI GTAC 1 cut(s) 505
AfiI CCNNNNNNNGG 1 cut(s) 529
AgsI TTSAA 4 cut(s) 88, 367, 540, 694
AhlI ACTAGT 2 cut(s) 164, 676
AjuI GAANNNNNNNTTGG 2 cut(s) 248, 280
AluBI AGCT 6 cut(s) 69, 312, 376, 397, 627, 685
AluI AGCT 6 cut(s) 69, 312, 376, 397, 627, 685
AlwI GGATC 2 cut(s) 334, 617
AoxI GGCC 2 cut(s) 72, 520
ApeKI GCWGC 4 cut(s) 117, 288, 299, 353
Asp700I GAANNNNTTC 1 cut(s) 451
AspLEI GCGC 1 cut(s) 42
AsuNHI GCTAGC 1 cut(s) 308
BalI TGGCCA 1 cut(s) 74
BanI GGYRCC 1 cut(s) 317
BbvI GCAGC 4 cut(s) 129, 275, 286, 365
BccI CCATC 1 cut(s) 594
BceAI ACGGC 1 cut(s) 179
BclI TGATCA 1 cut(s) 391
BcuI ACTAGT 2 cut(s) 164, 676
BfaI CTAG 4 cut(s) 165, 309, 677, 686
BfmI CTRYAG 1 cut(s) 628
BisI GCNGC 4 cut(s) 118, 289, 300, 354
BlsI GCNGC 4 cut(s) 119, 290, 301, 355
BmiI GGNNCC 2 cut(s) 319, 584
BmrI ACTGGG 1 cut(s) 284
BmsI GCATC 3 cut(s) 51, 406, 481
BmtI GCTAGC 1 cut(s) 312
BmuI ACTGGG 1 cut(s) 284
BpuEI CTTGAG 1 cut(s) 203
Bsc4I CCNNNNNNNGG 1 cut(s) 529
Bse1I ACTGG 1 cut(s) 279
Bse3DI GCAATG 1 cut(s) 127
BseGI GGATG 3 cut(s) 496, 586, 670
BseLI CCNNNNNNNGG 1 cut(s) 529
BseMI GCAATG 1 cut(s) 127
BseMII CTCAG 2 cut(s) 221, 585
BseNI ACTGG 1 cut(s) 279
BseXI GCAGC 4 cut(s) 129, 275, 286, 365
BseYI CCCAGC 1 cut(s) 494
BsgI GTGCAG 1 cut(s) 274
Bsh1236I CGCG 1 cut(s) 40
BshFI GGCC 2 cut(s) 74, 522
BshNI GGYRCC 1 cut(s) 317
BslFI GGGAC 2 cut(s) 291, 590
BslI CCNNNNNNNGG 1 cut(s) 529
BsmFI GGGAC 2 cut(s) 291, 590
BsnI GGCC 2 cut(s) 74, 522
Bsp1407I TGTACA 1 cut(s) 503
Bsp143I GATC 3 cut(s) 326, 391, 609
BspACI CCGC 1 cut(s) 516
BspANI GGCC 2 cut(s) 74, 522
BspCNI CTCAG 2 cut(s) 222, 586
BspFNI CGCG 1 cut(s) 40
BspLI GGNNCC 2 cut(s) 319, 584
BspOI GCTAGC 1 cut(s) 312
BspPI GGATC 2 cut(s) 334, 617
BspT107I GGYRCC 1 cut(s) 317
BsrDI GCAATG 1 cut(s) 127
BsrGI TGTACA 1 cut(s) 503
BsrI ACTGG 1 cut(s) 279
BssMI GATC 3 cut(s) 326, 391, 609
Bst4CI ACNGT 3 cut(s) 12, 484, 632
BstAUI TGTACA 1 cut(s) 503
BstC8I GCNNGC 2 cut(s) 53, 310
BstDEI CTNAG 2 cut(s) 230, 594
BstF5I GGATG 3 cut(s) 496, 586, 670
BstFNI CGCG 1 cut(s) 40
BstHHI GCGC 1 cut(s) 42
BstKTI GATC 3 cut(s) 329, 394, 612
BstMBI GATC 3 cut(s) 326, 391, 609
BstMWI GCNNNNNNNGC 2 cut(s) 48, 305
BstNSI RCATGY 1 cut(s) 55
BstSFI CTRYAG 1 cut(s) 628
BstUI CGCG 1 cut(s) 40
BstV1I GCAGC 4 cut(s) 129, 275, 286, 365
BsuRI GGCC 2 cut(s) 74, 522
BtsCI GGATG 3 cut(s) 496, 586, 670
BtsIMutI CAGTG 1 cut(s) 619
Cac8I GCNNGC 2 cut(s) 53, 310
CfoI GCGC 1 cut(s) 42
Csp6I GTAC 1 cut(s) 504
CviAII CATG 3 cut(s) 52, 330, 546
CviQI GTAC 1 cut(s) 504
DdeI CTNAG 2 cut(s) 230, 594
DpnI GATC 3 cut(s) 328, 393, 611
DpnII GATC 3 cut(s) 326, 391, 609
EaeI YGGCCR 1 cut(s) 72
Eco57I CTGAAG 1 cut(s) 357
FaeI CATG 3 cut(s) 55, 333, 549
FalI AAGNNNNNCTT 2 cut(s) 53, 85
FaqI GGGAC 2 cut(s) 291, 590
FatI CATG 3 cut(s) 51, 329, 545
FbaI TGATCA 1 cut(s) 391
Fnu4HI GCNGC 4 cut(s) 118, 289, 300, 354
FokI GGATG 3 cut(s) 503, 573, 677
Fsp4HI GCNGC 4 cut(s) 118, 289, 300, 354
FspBI CTAG 4 cut(s) 165, 309, 677, 686
GlaI GCGC 1 cut(s) 41
GluI GCNGC 4 cut(s) 118, 289, 300, 354
GsaI CCCAGC 1 cut(s) 498
HaeIII GGCC 2 cut(s) 74, 522
HhaI GCGC 1 cut(s) 42
Hin1II CATG 3 cut(s) 55, 333, 549
Hin6I GCGC 1 cut(s) 40
HinP1I GCGC 1 cut(s) 40
HincII GTYRAC 1 cut(s) 674
HindII GTYRAC 1 cut(s) 674
HindIII AAGCTT 1 cut(s) 67
HinfI GANTC 5 cut(s) 447, 477, 536, 563, 641
Hpy166II GTNNAC 4 cut(s) 8, 272, 506, 674
Hpy188I TCNGA 3 cut(s) 337, 490, 609
Hpy188III TCNNGA 1 cut(s) 170
Hpy8I GTNNAC 4 cut(s) 8, 272, 506, 674
HpyAV CCTTC 1 cut(s) 250
HpyCH4III ACNGT 3 cut(s) 12, 484, 632
HpyCH4V TGCA 4 cut(s) 291, 302, 353, 419
HpyF10VI GCNNNNNNNGC 2 cut(s) 48, 305
HpyF3I CTNAG 2 cut(s) 230, 594
Hsp92II CATG 3 cut(s) 55, 333, 549
HspAI GCGC 1 cut(s) 40
Ksp22I TGATCA 1 cut(s) 391
Kzo9I GATC 3 cut(s) 326, 391, 609
LmnI GCTCC 1 cut(s) 248
LpnPI CCDG 8 cut(s) 10, 88, 99, 260, 309, 480, 536, 686
Lsp1109I GCAGC 4 cut(s) 129, 275, 286, 365
LweI GCATC 3 cut(s) 51, 406, 481
MaeI CTAG 4 cut(s) 165, 309, 677, 686
MalI GATC 3 cut(s) 328, 393, 611
MboI GATC 3 cut(s) 326, 391, 609
MboII GAAGA 2 cut(s) 350, 545
MfeI CAATTG 1 cut(s) 303
MlsI TGGCCA 1 cut(s) 74
MluCI AATT 3 cut(s) 136, 303, 406
MluNI TGGCCA 1 cut(s) 74
MlyI GAGTC 2 cut(s) 572, 650
MmeI TCCRAC 1 cut(s) 468
MnlI CCTC 5 cut(s) 159, 178, 544, 589, 708
Mox20I TGGCCA 1 cut(s) 74
MroXI GAANNNNTTC 1 cut(s) 451
MscI TGGCCA 1 cut(s) 74
MseI TTAA 5 cut(s) 14, 132, 435, 510, 603
MslI CAYNNNNRTG 1 cut(s) 50
Msp20I TGGCCA 1 cut(s) 74
MspA1I CMGCKG 1 cut(s) 516
MunI CAATTG 1 cut(s) 303
MvnI CGCG 1 cut(s) 40
MwoI GCNNNNNNNGC 2 cut(s) 48, 305
NdeII GATC 3 cut(s) 326, 391, 609
NheI GCTAGC 1 cut(s) 308
NlaIII CATG 3 cut(s) 55, 333, 549
NlaIV GGNNCC 2 cut(s) 319, 584
NmeAIII GCCGAG 1 cut(s) 199
NspI RCATGY 1 cut(s) 55
PaeI GCATGC 1 cut(s) 55
PdmI GAANNNNTTC 1 cut(s) 451
PfeI GAWTC 3 cut(s) 447, 477, 536
PkrI GCNGC 4 cut(s) 119, 290, 301, 355
PleI GAGTC 2 cut(s) 571, 649
PpsI GAGTC 2 cut(s) 571, 649
PspFI CCCAGC 1 cut(s) 494
PspN4I GGNNCC 2 cut(s) 319, 584
RsaI GTAC 1 cut(s) 505
RsaNI GTAC 1 cut(s) 504
RseI CAYNNNNRTG 1 cut(s) 50
SaqAI TTAA 5 cut(s) 14, 132, 435, 510, 603
SatI GCNGC 4 cut(s) 118, 289, 300, 354
Sau3AI GATC 3 cut(s) 326, 391, 609
SchI GAGTC 2 cut(s) 572, 650
SetI ASST 8 cut(s) 71, 151, 261, 314, 378, 399, 629, 687
SfaNI GCATC 3 cut(s) 51, 406, 481
SfcI CTRYAG 1 cut(s) 628
SmiMI CAYNNNNRTG 1 cut(s) 50
SmlI CTYRAG 1 cut(s) 182
SmoI CTYRAG 1 cut(s) 182
SpeI ACTAGT 2 cut(s) 164, 676
SphI GCATGC 1 cut(s) 55
Sse9I AATT 3 cut(s) 136, 303, 406
SsiI CCGC 1 cut(s) 516
SspI AATATT 1 cut(s) 215
SspMI CTAG 4 cut(s) 165, 309, 677, 686
TaaI ACNGT 3 cut(s) 12, 484, 632
TaqI TCGA 1 cut(s) 207
TaqII GACCGA 1 cut(s) 445
TasI AATT 3 cut(s) 136, 303, 406
TatI WGTACW 1 cut(s) 503
TfiI GAWTC 3 cut(s) 447, 477, 536
Tru1I TTAA 5 cut(s) 14, 132, 435, 510, 603
Tru9I TTAA 5 cut(s) 14, 132, 435, 510, 603
TscAI CASTG 1 cut(s) 619
TseI GCWGC 4 cut(s) 117, 288, 299, 353
TspDTI ATGAA 1 cut(s) 415
TspGWI ACGGA 2 cut(s) 339, 459
TspRI CASTG 1 cut(s) 619
XceI RCATGY 1 cut(s) 55
XmnI GAANNNNTTC 1 cut(s) 451
XspI CTAG 4 cut(s) 165, 309, 677, 686
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.