Rh5AG096100

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0001843
Physical Location & Seq
Reverse (-)
8855614 .. 8858715
3102 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG096100.1

Sequence Viewer

Length: 1086 bp
ATGGCTTTTCAAGTGAAACCATTGGCGGTGGTAATTGCTCTATCTTGGGTTTTAAGCATGCAACAGCATTCTTCTTCTGTTAATGGAAAGCCACAAGTACCTTGTTTCTTCATTTTTGGAGACTCGCTGGCTGACAGTGGCAACAACAACTTGCTTGATTCTCTGGCTAAAGTCAACTACACGCCGTATGGTATCGACTTCCCTGGTGGCGCAACCGGAAGATTTACCAACGGTCGAACCACCATCGACGTACTATCAGGAATTCGCAAAGAAAGCGGGAGGCAATTGGGTGCAAGAATCAGCATGGACAGACAGTTAAAGAATCACAAGATTACAATCTCCCGGATTGCTGACATACTTGGAAAGAAGAGGGCTAAAAGTCCCTACTTAAGCAAGTACTTGTACAAGTCCTTACGTTCAGGTGTAAAAAAGTGGTTGGCCAAAAAATACTTAAGCAAGTGCTTGTATTCAGTTGGAATGGGCAGTAATGACTACATCAACAATTACTTCTTGCCTCAGTACCATGACACCAGTAAAAAGTTTACCACTGAGCAATATGCTGAAGCCCTTATCAAACAATATTCTCAGCAAATAAAGAAGTTGCACAAATATGGTGCAAGGAAGGTGGTTTTGGTTGGAGTGGGACTGATAGGCTGCACCCCAACTGCAATTTCTGCTGGCACAAATGGGTCGGCATGTGTAGATATAATGAACCTTGCAGCTCTACAGTTTAACCAAAGGCTTGTATCACTTGTGGATCAGCTCAACAGCAATTTGACAAATGCAATGTTCATCTATATTAACAGCTTTGAAATGGGTTCTGGAGATCCAGCAGCTGCTGGATTTAAGGTTTCGAACGTTGGGTGTTGTGCGGTAAATGAAGTTGGTCAATGTAAATTTGACCAAAATCCATGCAAGAATAGGAGCGAGTATGTGTTTTGGGACGGATTCCATCCTACAGAAGCCTTGAACCAAATCAATGCAATAAGATCATACAGTAGTGCTATTAATTCTGCAGACACTTATCCAATGGACATCAGTCACCTTGCTAAGCTAAAATTTAATCATAGAGGGGCTGCTTATTAG

Protein Analysis

361

Amino Acids

39.81

Weight (kDa)

9.54

Isoelectric Point (pI)

20.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 37 - 326 2.7e-27 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 26, 276, 872
AclI AACGTT 1 cut(s) 858
AclWI GGATC 2 cut(s) 765, 821
AcoI YGGCCR 1 cut(s) 438
AcsI RAATTY 3 cut(s) 261, 896, 1058
AcuI CTGAAG 1 cut(s) 582
AfaI GTAC 5 cut(s) 99, 252, 398, 404, 521
AflII CTTAAG 2 cut(s) 388, 451
AgsI TTSAA 3 cut(s) 11, 812, 970
AjnI CCWGG 1 cut(s) 202
AjuI GAANNNNNNNTTGG 4 cut(s) 614, 646, 655, 687
AluBI AGCT 5 cut(s) 722, 763, 807, 836, 1054
AluI AGCT 5 cut(s) 722, 763, 807, 836, 1054
Alw26I GTCTC 1 cut(s) 114
AlwI GGATC 2 cut(s) 765, 821
AlwNI CAGNNNCTG 2 cut(s) 836, 839
AoxI GGCC 1 cut(s) 438
ApeKI GCWGC 5 cut(s) 654, 719, 833, 836, 1076
ApoI RAATTY 3 cut(s) 261, 896, 1058
AseI ATTAAT 1 cut(s) 1008
AspLEI GCGC 1 cut(s) 212
AsuC2I CCSGG 1 cut(s) 343
AsuHPI GGTGA 1 cut(s) 1034
AsuII TTCGAA 1 cut(s) 854
BalI TGGCCA 1 cut(s) 440
BbvI GCAGC 5 cut(s) 641, 731, 823, 845, 1063
BccI CCATC 2 cut(s) 251, 960
BceAI ACGGC 1 cut(s) 169
BciT130I CCWGG 1 cut(s) 204
BcnI CCSGG 1 cut(s) 343
BcoDI GTCTC 1 cut(s) 114
BfmI CTRYAG 3 cut(s) 725, 957, 1014
BfrI CTTAAG 2 cut(s) 388, 451
BisI GCNGC 5 cut(s) 655, 720, 834, 837, 1077
BlpI GCTNAGC 1 cut(s) 1050
BlsI GCNGC 5 cut(s) 656, 721, 835, 838, 1078
BmcAI AGTACT 1 cut(s) 398
Bme1390I CCNGG 2 cut(s) 204, 343
BmrFI CCNGG 2 cut(s) 204, 343
BoxI GACNNNNGTC 1 cut(s) 1038
BpmI CTGGAG 1 cut(s) 843
Bpu1102I GCTNAGC 1 cut(s) 1050
Bpu14I TTCGAA 1 cut(s) 854
BpuMI CCSGG 1 cut(s) 343
BsaBI GATNNNNATC 1 cut(s) 335
BsaJI CCNNGG 1 cut(s) 202
BsaWI WCCGGW 1 cut(s) 215
Bse1I ACTGG 1 cut(s) 531
Bse3DI GCAATG 1 cut(s) 792
Bse8I GATNNNNATC 1 cut(s) 335
BseBI CCWGG 1 cut(s) 204
BseDI CCNNGG 1 cut(s) 202
BseGI GGATG 1 cut(s) 952
BseJI GATNNNNATC 1 cut(s) 335
BseMI GCAATG 1 cut(s) 792
BseMII CTCAG 3 cut(s) 530, 540, 599
BseNI ACTGG 1 cut(s) 531
BseXI GCAGC 5 cut(s) 641, 731, 823, 845, 1063
BsgI GTGCAG 1 cut(s) 640
Bsh1285I CGRYCG 1 cut(s) 235
BshFI GGCC 1 cut(s) 440
BsiEI CGRYCG 1 cut(s) 235
BsiSI CCGG 2 cut(s) 216, 343
BslFI GGGAC 3 cut(s) 366, 657, 956
BsmAI GTCTC 1 cut(s) 114
BsmFI GGGAC 3 cut(s) 366, 657, 956
BsmI GAATGC 1 cut(s) 67
BsnI GGCC 1 cut(s) 440
Bsp119I TTCGAA 1 cut(s) 854
Bsp1407I TGTACA 1 cut(s) 402
Bsp143I GATC 3 cut(s) 757, 826, 989
Bsp1720I GCTNAGC 1 cut(s) 1050
BspACI CCGC 3 cut(s) 26, 276, 872
BspANI GGCC 1 cut(s) 440
BspCNI CTCAG 3 cut(s) 529, 541, 598
BspMAI CTGCAG 1 cut(s) 1018
BspPI GGATC 2 cut(s) 765, 821
BspT104I TTCGAA 1 cut(s) 854
BspTI CTTAAG 2 cut(s) 388, 451
BsrDI GCAATG 1 cut(s) 792
BsrGI TGTACA 1 cut(s) 402
BsrI ACTGG 1 cut(s) 531
BssECI CCNNGG 1 cut(s) 202
BssMI GATC 3 cut(s) 757, 826, 989
Bst2UI CCWGG 1 cut(s) 204
Bst4CI ACNGT 5 cut(s) 137, 233, 315, 729, 998
Bst6I CTCTTC 1 cut(s) 362
BstAFI CTTAAG 2 cut(s) 388, 451
BstAPI GCANNNNNTGC 1 cut(s) 674
BstAUI TGTACA 1 cut(s) 402
BstBI TTCGAA 1 cut(s) 854
BstC8I GCNNGC 3 cut(s) 59, 129, 679
BstDEI CTNAG 4 cut(s) 516, 549, 585, 1050
BstF5I GGATG 1 cut(s) 952
BstHHI GCGC 1 cut(s) 212
BstKTI GATC 3 cut(s) 760, 829, 992
BstMAI GTCTC 1 cut(s) 114
BstMBI GATC 3 cut(s) 757, 826, 989
BstMCI CGRYCG 1 cut(s) 235
BstMWI GCNNNNNNNGC 2 cut(s) 273, 674
BstNI CCWGG 1 cut(s) 204
BstNSI RCATGY 2 cut(s) 61, 699
BstPAI GACNNNNGTC 1 cut(s) 1038
BstSCI CCNGG 2 cut(s) 202, 341
BstSFI CTRYAG 3 cut(s) 725, 957, 1014
BstV1I GCAGC 5 cut(s) 641, 731, 823, 845, 1063
BstX2I RGATCY 1 cut(s) 826
BstYI RGATCY 1 cut(s) 826
BsuRI GGCC 1 cut(s) 440
BtsCI GGATG 1 cut(s) 952
BtsIMutI CAGTG 2 cut(s) 142, 546
Cac8I GCNNGC 3 cut(s) 59, 129, 679
CaiI CAGNNNCTG 2 cut(s) 836, 839
CfoI GCGC 1 cut(s) 212
Csp6I GTAC 5 cut(s) 98, 251, 397, 403, 520
CspCI CAANNNNNGTGG 2 cut(s) 606, 641
CviAII CATG 5 cut(s) 58, 304, 524, 696, 912
CviQI GTAC 5 cut(s) 98, 251, 397, 403, 520
DdeI CTNAG 4 cut(s) 516, 549, 585, 1050
DpnI GATC 3 cut(s) 759, 828, 991
DpnII GATC 3 cut(s) 757, 826, 989
EaeI YGGCCR 1 cut(s) 438
Eam1104I CTCTTC 1 cut(s) 362
EarI CTCTTC 1 cut(s) 362
Eco57I CTGAAG 1 cut(s) 582
EcoRI GAATTC 1 cut(s) 261
EcoRII CCWGG 1 cut(s) 202
FaeI CATG 5 cut(s) 61, 307, 527, 699, 915
FaqI GGGAC 3 cut(s) 366, 657, 956
FatI CATG 5 cut(s) 57, 303, 523, 695, 911
FauI CCCGC 1 cut(s) 269
Fnu4HI GCNGC 5 cut(s) 655, 720, 834, 837, 1077
FokI GGATG 1 cut(s) 939
Fsp4HI GCNGC 5 cut(s) 655, 720, 834, 837, 1077
GlaI GCGC 1 cut(s) 211
GluI GCNGC 5 cut(s) 655, 720, 834, 837, 1077
GsuI CTGGAG 1 cut(s) 843
HaeIII GGCC 1 cut(s) 440
HapII CCGG 2 cut(s) 216, 343
HhaI GCGC 1 cut(s) 212
Hin1II CATG 5 cut(s) 61, 307, 527, 699, 915
Hin6I GCGC 1 cut(s) 210
HinP1I GCGC 1 cut(s) 210
HincII GTYRAC 1 cut(s) 175
HindII GTYRAC 1 cut(s) 175
HinfI GANTC 5 cut(s) 122, 158, 297, 322, 948
HpaII CCGG 2 cut(s) 216, 343
HphI GGTGA 1 cut(s) 1034
Hpy166II GTNNAC 2 cut(s) 175, 543
Hpy188III TCNNGA 2 cut(s) 258, 822
Hpy8I GTNNAC 2 cut(s) 175, 543
Hpy99I CGWCG 1 cut(s) 251
HpyAV CCTTC 1 cut(s) 616
HpyCH4III ACNGT 5 cut(s) 137, 233, 315, 729, 998
HpyCH4IV ACGT 3 cut(s) 249, 415, 858
HpyF10VI GCNNNNNNNGC 2 cut(s) 273, 674
HpyF3I CTNAG 4 cut(s) 516, 549, 585, 1050
HpySE526I ACGT 3 cut(s) 249, 415, 858
Hsp92II CATG 5 cut(s) 61, 307, 527, 699, 915
HspAI GCGC 1 cut(s) 210
Kzo9I GATC 3 cut(s) 757, 826, 989
LmnI GCTCC 1 cut(s) 924
Lsp1109I GCAGC 5 cut(s) 641, 731, 823, 845, 1063
MaeII ACGT 3 cut(s) 249, 415, 858
MaeIII GTNAC 1 cut(s) 1040
MalI GATC 3 cut(s) 759, 828, 991
MboI GATC 3 cut(s) 757, 826, 989
MboII GAAGA 5 cut(s) 63, 66, 100, 231, 379
MfeI CAATTG 1 cut(s) 284
MflI RGATCY 1 cut(s) 826
MlsI TGGCCA 1 cut(s) 440
MluCI AATT 9 cut(s) 33, 261, 284, 502, 669, 772, 896, 1009, 1058
MluNI TGGCCA 1 cut(s) 440
MlyI GAGTC 1 cut(s) 116
MmeI TCCRAC 2 cut(s) 454, 616
MnlI CCTC 4 cut(s) 273, 363, 525, 1064
Mox20I TGGCCA 1 cut(s) 440
MscI TGGCCA 1 cut(s) 440
MslI CAYNNNNRTG 1 cut(s) 609
Msp20I TGGCCA 1 cut(s) 440
MspA1I CMGCKG 1 cut(s) 836
MspCI CTTAAG 2 cut(s) 388, 451
MspI CCGG 2 cut(s) 216, 343
MspR9I CCNGG 2 cut(s) 204, 343
MunI CAATTG 1 cut(s) 284
Mva1269I GAATGC 1 cut(s) 67
MvaI CCWGG 1 cut(s) 204
MwoI GCNNNNNNNGC 2 cut(s) 273, 674
NciI CCSGG 1 cut(s) 343
NdeII GATC 3 cut(s) 757, 826, 989
NlaIII CATG 5 cut(s) 61, 307, 527, 699, 915
NmuCI GTSAC 1 cut(s) 1040
NspI RCATGY 2 cut(s) 61, 699
NspV TTCGAA 1 cut(s) 854
PaeI GCATGC 1 cut(s) 61
PctI GAATGC 1 cut(s) 67
PfeI GAWTC 4 cut(s) 158, 297, 322, 948
PfoI TCCNGGA 1 cut(s) 341
PkrI GCNGC 5 cut(s) 656, 721, 835, 838, 1078
PleI GAGTC 1 cut(s) 116
PpsI GAGTC 1 cut(s) 116
PshAI GACNNNNGTC 1 cut(s) 1038
PshBI ATTAAT 1 cut(s) 1008
Psp1406I AACGTT 1 cut(s) 858
Psp6I CCWGG 1 cut(s) 202
PspGI CCWGG 1 cut(s) 202
PstI CTGCAG 1 cut(s) 1018
PstNI CAGNNNCTG 2 cut(s) 836, 839
PsuI RGATCY 1 cut(s) 826
PvuII CAGCTG 1 cut(s) 836
RsaI GTAC 5 cut(s) 99, 252, 398, 404, 521
RsaNI GTAC 5 cut(s) 98, 251, 397, 403, 520
RseI CAYNNNNRTG 1 cut(s) 609
SatI GCNGC 5 cut(s) 655, 720, 834, 837, 1077
Sau3AI GATC 3 cut(s) 757, 826, 989
ScaI AGTACT 1 cut(s) 398
SchI GAGTC 1 cut(s) 116
ScrFI CCNGG 2 cut(s) 204, 343
SfcI CTRYAG 3 cut(s) 725, 957, 1014
SfuI TTCGAA 1 cut(s) 854
SmiMI CAYNNNNRTG 1 cut(s) 609
SmlI CTYRAG 2 cut(s) 388, 451
SmoI CTYRAG 2 cut(s) 388, 451
SphI GCATGC 1 cut(s) 61
Sse9I AATT 9 cut(s) 33, 261, 284, 502, 669, 772, 896, 1009, 1058
SsiI CCGC 3 cut(s) 26, 276, 872
SspI AATATT 1 cut(s) 581
StyD4I CCNGG 2 cut(s) 202, 341
TaaI ACNGT 5 cut(s) 137, 233, 315, 729, 998
TaiI ACGT 3 cut(s) 252, 418, 861
TaqI TCGA 4 cut(s) 195, 235, 246, 854
TasI AATT 9 cut(s) 33, 261, 284, 502, 669, 772, 896, 1009, 1058
TatI WGTACW 2 cut(s) 396, 402
TfiI GAWTC 4 cut(s) 158, 297, 322, 948
TscAI CASTG 2 cut(s) 142, 553
TseFI GTSAC 1 cut(s) 1040
TseI GCWGC 5 cut(s) 654, 719, 833, 836, 1076
Tsp45I GTSAC 1 cut(s) 1040
TspDTI ATGAA 4 cut(s) 100, 725, 781, 894
TspGWI ACGGA 1 cut(s) 960
TspRI CASTG 2 cut(s) 142, 553
Vha464I CTTAAG 2 cut(s) 388, 451
VspI ATTAAT 1 cut(s) 1008
XapI RAATTY 3 cut(s) 261, 896, 1058
XceI RCATGY 2 cut(s) 61, 699
ZrmI AGTACT 1 cut(s) 398
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.