RLG00000031920

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0001843
Physical Location & Seq
Reverse (-)
9495774 .. 9499371
3598 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000031920

Sequence Viewer

Length: 1323 bp
ATGGTTGTGGTGGAGAGCTCGGTGGTGGTTATGGGTGTAGTGATAGGTGGAGTAGGAGGTGGTGCAGCAGTGAGTGGTGGTGGTGAGAGGATGGTTTTAGTGGTGGTGACATTGAAACTGCTCTTAGGCCATCAGCCATGGCCCCTACACGGCGGCTCGGCCTATGTCCGTCATATAAAAACGCCCACCGAGCTTTCTGCAATGGCTTGTGGGATGAAGCCGTGGGTGGTGGTGATTGCTCTGACTTGGTTTTTAAGCCTGCAAGAACATTCTTCTTCTGTTCATGGAAAGCCACAAGTACCTTGTTTCTTCATTTTTGGAGACTCACTGGCTGATAATGGCAACAACAACGTCCTTGATACTTTGGCTAAAGTCAATTACCAGCCTTATGGTATCGACTTCCCTAGTGGCACAGCAACCGGAAGATTCAGCAACGGTCGAACCACCGTCGATGTTCTCTCTGAACTTTTAGGCTTTGACAACTACATTCCACCATTTGCTTTTGTTAATGGCTCTGACATACTTAAAGGTGTGAACTATGCTTGTGGCGCTGCAGGAATTCGAAAAGAAACTGGCAAGCAATTGGGTGCAAGAATCAGCATGGGCAGACAGTTAAAGAACCACAAGATTATAATCTCACGCATTGTCGACATACTTGGGAAGAAGAGCTTAGCGCAAAAGCACTTAAACAAGTGCTTATATTCAGTTGGAATGGGCAGCAATGACTACATCAACAATTACTTCGTGCCTCAGTATTACGACACCAGCAAAAAATTTACCACTGAGCAATATGCTGAAGTTCTCATCAACCAATATTCTTGGGAAATACTGAAGTTGTACAAGTATGGAGCAAGGAAGGTGGCTTTGGTTGGATTGGGACTAATAGGCTGCACCCCAAGTGCAATTTCTTCCTTTGGCACAAATGGGTCAGCATGTGTAGACACATTGAACATTGCAGCGCAACTGTTTAACCAAAGGCTTGTATCACTTGTGGACAAGCTCAACAGCAATTTGACAGATGCAAAATTCATCTATATTAACAGCTTTGAAATGGGTTCTGGAGATCCTGCAGCTGCTGGCTTCACAGTTTCGAATGTTGGGTGTTGTGCGGTAAATGAAGTTGGTCAATGTAATGTTGACCATACTCCATGCAAGAACAGGACTGAGTACGTGTTCTGGGACGGATTCCATCCTACTGAAGCCTTGAACCGAATCACTGCAATCAGATCATACAATGCTTTTAACCCTGTAGACACTTATCCAATGGATATTAGTCACCTTGTTCAGCTACAAATTAATAACCCAGCAACTCCAGCTACCTAG

Protein Analysis

441

Amino Acids

47.6

Weight (kDa)

8.41

Isoelectric Point (pI)

23.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 104 - 407 3.2e-33 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 632
AccI GTMKAC 3 cut(s) 648, 939, 1251
AciI CCGC 2 cut(s) 153, 1109
AclWI GGATC 1 cut(s) 1058
AcsI RAATTY 3 cut(s) 558, 773, 1025
AcuI CTGAAG 3 cut(s) 816, 851, 1218
AfaI GTAC 3 cut(s) 300, 839, 1169
AfiI CCNNNNNNNGG 1 cut(s) 149
AflIII ACRYGT 1 cut(s) 1170
AgsI TTSAA 4 cut(s) 115, 949, 1049, 1207
AjuI GAANNNNNNNTTGG 4 cut(s) 848, 880, 889, 921
AluBI AGCT 8 cut(s) 18, 193, 669, 1000, 1044, 1073, 1288, 1316
AluI AGCT 8 cut(s) 18, 193, 669, 1000, 1044, 1073, 1288, 1316
Alw21I GWGCWC 1 cut(s) 20
Alw26I GTCTC 1 cut(s) 315
AlwI GGATC 1 cut(s) 1058
AlwNI CAGNNNCTG 1 cut(s) 1076
AoxI GGCC 3 cut(s) 127, 140, 159
ApeKI GCWGC 7 cut(s) 65, 551, 717, 888, 956, 1070, 1073
ApoI RAATTY 3 cut(s) 558, 773, 1025
AseI ATTAAT 1 cut(s) 1296
AspLEI GCGC 3 cut(s) 551, 676, 961
AspS9I GGNCC 1 cut(s) 141
AsuHPI GGTGA 4 cut(s) 95, 118, 244, 1268
AsuII TTCGAA 2 cut(s) 562, 1091
BanII GRGCYC 1 cut(s) 20
Bbv12I GWGCWC 1 cut(s) 20
BbvI GCAGC 7 cut(s) 77, 538, 729, 875, 968, 1060, 1082
BccI CCATC 3 cut(s) 85, 138, 1197
BceAI ACGGC 2 cut(s) 166, 205
BcgI CGANNNNNNTGC 2 cut(s) 179, 213
BcoDI GTCTC 1 cut(s) 315
BfaI CTAG 2 cut(s) 405, 1321
BfmI CTRYAG 3 cut(s) 552, 1068, 1248
BfoI RGCGCY 1 cut(s) 552
BisI GCNGC 8 cut(s) 66, 154, 552, 718, 889, 957, 1071, 1074
BlpI GCTNAGC 1 cut(s) 670
BlsI GCNGC 8 cut(s) 67, 155, 553, 719, 890, 958, 1072, 1075
BmgT120I GGNCC 1 cut(s) 141
BmiI GGNNCC 1 cut(s) 143
BmsI GCATC 1 cut(s) 1009
BpmI CTGGAG 2 cut(s) 1080, 1296
Bpu1102I GCTNAGC 1 cut(s) 670
Bpu14I TTCGAA 2 cut(s) 562, 1091
BsaAI YACGTR 1 cut(s) 1171
BsaBI GATNNNNATC 1 cut(s) 632
BsaJI CCNNGG 2 cut(s) 137, 221
BsaWI WCCGGW 1 cut(s) 419
Bsc4I CCNNNNNNNGG 1 cut(s) 149
Bse1I ACTGG 2 cut(s) 333, 577
Bse3DI GCAATG 3 cut(s) 207, 727, 951
Bse8I GATNNNNATC 1 cut(s) 632
BseDI CCNNGG 2 cut(s) 137, 221
BseGI GGATG 3 cut(s) 96, 219, 1189
BseJI GATNNNNATC 1 cut(s) 632
BseLI CCNNNNNNNGG 1 cut(s) 149
BseMI GCAATG 3 cut(s) 207, 727, 951
BseMII CTCAG 3 cut(s) 764, 774, 1155
BseNI ACTGG 2 cut(s) 333, 577
BseXI GCAGC 7 cut(s) 77, 538, 729, 875, 968, 1060, 1082
BseYI CCCAGC 1 cut(s) 1303
BsgI GTGCAG 2 cut(s) 84, 874
Bsh1285I CGRYCG 1 cut(s) 439
BshFI GGCC 3 cut(s) 129, 142, 161
BsiEI CGRYCG 1 cut(s) 439
BsiHKAI GWGCWC 1 cut(s) 20
BsiSI CCGG 1 cut(s) 420
BslFI GGGAC 2 cut(s) 891, 1193
BslI CCNNNNNNNGG 1 cut(s) 149
BsmAI GTCTC 1 cut(s) 315
BsmFI GGGAC 2 cut(s) 891, 1193
BsnI GGCC 3 cut(s) 129, 142, 161
Bsp119I TTCGAA 2 cut(s) 562, 1091
Bsp1286I GDGCHC 1 cut(s) 20
Bsp1407I TGTACA 1 cut(s) 837
Bsp143I GATC 2 cut(s) 1063, 1226
Bsp1720I GCTNAGC 1 cut(s) 670
Bsp19I CCATGG 1 cut(s) 137
BspACI CCGC 2 cut(s) 153, 1109
BspANI GGCC 3 cut(s) 129, 142, 161
BspCNI CTCAG 3 cut(s) 763, 775, 1156
BspLI GGNNCC 1 cut(s) 143
BspMAI CTGCAG 2 cut(s) 556, 1072
BspPI GGATC 1 cut(s) 1058
BspQI GCTCTTC 1 cut(s) 659
BspT104I TTCGAA 2 cut(s) 562, 1091
BsrDI GCAATG 3 cut(s) 207, 727, 951
BsrGI TGTACA 1 cut(s) 837
BsrI ACTGG 2 cut(s) 333, 577
BssECI CCNNGG 2 cut(s) 137, 221
BssMI GATC 2 cut(s) 1063, 1226
BssT1I CCWWGG 1 cut(s) 137
Bst4CI ACNGT 5 cut(s) 437, 448, 612, 966, 1087
Bst6I CTCTTC 1 cut(s) 659
BstAUI TGTACA 1 cut(s) 837
BstBAI YACGTR 1 cut(s) 1171
BstBI TTCGAA 2 cut(s) 562, 1091
BstC8I GCNNGC 3 cut(s) 260, 578, 1078
BstDEI CTNAG 5 cut(s) 124, 670, 750, 783, 1164
BstDSI CCRYGG 2 cut(s) 137, 221
BstF5I GGATG 3 cut(s) 96, 219, 1189
BstH2I RGCGCY 1 cut(s) 552
BstHHI GCGC 3 cut(s) 551, 676, 961
BstKTI GATC 2 cut(s) 1066, 1229
BstMAI GTCTC 1 cut(s) 315
BstMBI GATC 2 cut(s) 1063, 1226
BstMCI CGRYCG 1 cut(s) 439
BstMWI GCNNNNNNNGC 3 cut(s) 190, 548, 1313
BstNSI RCATGY 1 cut(s) 936
BstSFI CTRYAG 3 cut(s) 552, 1068, 1248
BstV1I GCAGC 7 cut(s) 77, 538, 729, 875, 968, 1060, 1082
BstX2I RGATCY 1 cut(s) 1063
BstXI CCANNNNNNTGG 1 cut(s) 389
BstYI RGATCY 1 cut(s) 1063
BsuRI GGCC 3 cut(s) 129, 142, 161
BtgI CCRYGG 2 cut(s) 137, 221
BtsCI GGATG 3 cut(s) 96, 219, 1189
BtsI GCAGTG 2 cut(s) 75, 1215
BtsIMutI CAGTG 4 cut(s) 75, 326, 780, 1215
Cac8I GCNNGC 3 cut(s) 260, 578, 1078
CaiI CAGNNNCTG 1 cut(s) 1076
CfoI GCGC 3 cut(s) 551, 676, 961
Cfr13I GGNCC 1 cut(s) 141
Csp6I GTAC 3 cut(s) 299, 838, 1168
CspCI CAANNNNNGTGG 2 cut(s) 840, 875
CviAII CATG 5 cut(s) 138, 284, 601, 933, 1149
CviQI GTAC 3 cut(s) 299, 838, 1168
DdeI CTNAG 5 cut(s) 124, 670, 750, 783, 1164
DpnI GATC 2 cut(s) 1065, 1228
DpnII GATC 2 cut(s) 1063, 1226
Eam1104I CTCTTC 1 cut(s) 659
EarI CTCTTC 1 cut(s) 659
Ecl136II GAGCTC 1 cut(s) 18
Eco130I CCWWGG 1 cut(s) 137
Eco24I GRGCYC 1 cut(s) 20
Eco53kI GAGCTC 1 cut(s) 18
Eco57I CTGAAG 3 cut(s) 816, 851, 1218
EcoICRI GAGCTC 1 cut(s) 18
EcoRI GAATTC 1 cut(s) 558
EcoT14I CCWWGG 1 cut(s) 137
EcoT38I GRGCYC 1 cut(s) 20
ErhI CCWWGG 1 cut(s) 137
FaeI CATG 5 cut(s) 141, 287, 604, 936, 1152
FalI AAGNNNNNCTT 2 cut(s) 653, 685
FaqI GGGAC 2 cut(s) 891, 1193
FatI CATG 5 cut(s) 137, 283, 600, 932, 1148
FblI GTMKAC 3 cut(s) 648, 939, 1251
Fnu4HI GCNGC 8 cut(s) 66, 154, 552, 718, 889, 957, 1071, 1074
FokI GGATG 3 cut(s) 103, 226, 1176
FriOI GRGCYC 1 cut(s) 20
Fsp4HI GCNGC 8 cut(s) 66, 154, 552, 718, 889, 957, 1071, 1074
FspBI CTAG 2 cut(s) 405, 1321
GlaI GCGC 3 cut(s) 550, 675, 960
GluI GCNGC 8 cut(s) 66, 154, 552, 718, 889, 957, 1071, 1074
GsaI CCCAGC 1 cut(s) 1307
GsuI CTGGAG 2 cut(s) 1080, 1296
HaeII RGCGCY 1 cut(s) 552
HaeIII GGCC 3 cut(s) 129, 142, 161
HapII CCGG 1 cut(s) 420
HhaI GCGC 3 cut(s) 551, 676, 961
Hin1II CATG 5 cut(s) 141, 287, 604, 936, 1152
Hin6I GCGC 3 cut(s) 549, 674, 959
HinP1I GCGC 3 cut(s) 549, 674, 959
HincII GTYRAC 2 cut(s) 649, 1138
HindII GTYRAC 2 cut(s) 649, 1138
HinfI GANTC 5 cut(s) 323, 426, 594, 1185, 1212
HpaII CCGG 1 cut(s) 420
HphI GGTGA 4 cut(s) 95, 118, 244, 1268
Hpy166II GTNNAC 6 cut(s) 535, 649, 940, 994, 1138, 1252
Hpy188I TCNGA 4 cut(s) 243, 463, 517, 1226
Hpy188III TCNNGA 1 cut(s) 1059
Hpy8I GTNNAC 6 cut(s) 535, 649, 940, 994, 1138, 1252
Hpy99I CGWCG 1 cut(s) 452
HpyAV CCTTC 1 cut(s) 850
HpyCH4III ACNGT 5 cut(s) 437, 448, 612, 966, 1087
HpyCH4IV ACGT 2 cut(s) 351, 1170
HpyF10VI GCNNNNNNNGC 3 cut(s) 190, 548, 1313
HpyF3I CTNAG 5 cut(s) 124, 670, 750, 783, 1164
HpySE526I ACGT 2 cut(s) 351, 1170
Hsp92II CATG 5 cut(s) 141, 287, 604, 936, 1152
HspAI GCGC 3 cut(s) 549, 674, 959
Kzo9I GATC 2 cut(s) 1063, 1226
LguI GCTCTTC 1 cut(s) 659
LmnI GCTCC 1 cut(s) 848
Lsp1109I GCAGC 7 cut(s) 77, 538, 729, 875, 968, 1060, 1082
LweI GCATC 1 cut(s) 1009
MaeI CTAG 2 cut(s) 405, 1321
MaeII ACGT 2 cut(s) 351, 1170
MaeIII GTNAC 2 cut(s) 106, 1274
MalI GATC 2 cut(s) 1065, 1228
MboI GATC 2 cut(s) 1063, 1226
MboII GAAGA 7 cut(s) 264, 267, 301, 435, 673, 676, 900
MfeI CAATTG 1 cut(s) 581
MflI RGATCY 1 cut(s) 1063
MhlI GDGCHC 1 cut(s) 20
MluCI AATT 9 cut(s) 376, 558, 581, 736, 773, 903, 1009, 1025, 1293
MlyI GAGTC 1 cut(s) 317
MmeI TCCRAC 2 cut(s) 688, 850
MnlI CCTC 3 cut(s) 50, 81, 759
MseI TTAA 9 cut(s) 254, 507, 525, 614, 686, 969, 1038, 1242, 1296
MspA1I CMGCKG 1 cut(s) 1073
MspI CCGG 1 cut(s) 420
MunI CAATTG 1 cut(s) 581
MwoI GCNNNNNNNGC 3 cut(s) 190, 548, 1313
NcoI CCATGG 1 cut(s) 137
NdeII GATC 2 cut(s) 1063, 1226
NlaIII CATG 5 cut(s) 141, 287, 604, 936, 1152
NlaIV GGNNCC 1 cut(s) 143
NmeAIII GCCGAG 1 cut(s) 137
NmuCI GTSAC 2 cut(s) 106, 1274
NspI RCATGY 1 cut(s) 936
NspV TTCGAA 2 cut(s) 562, 1091
PciSI GCTCTTC 1 cut(s) 659
PfeI GAWTC 4 cut(s) 426, 594, 1185, 1212
PkrI GCNGC 8 cut(s) 67, 155, 553, 719, 890, 958, 1072, 1075
PleI GAGTC 1 cut(s) 317
PpsI GAGTC 1 cut(s) 317
Ppu21I YACGTR 1 cut(s) 1171
PshBI ATTAAT 1 cut(s) 1296
PsiI TTATAA 1 cut(s) 632
Psp124BI GAGCTC 1 cut(s) 20
PspFI CCCAGC 1 cut(s) 1303
PspN4I GGNNCC 1 cut(s) 143
PspPI GGNCC 1 cut(s) 141
PstI CTGCAG 2 cut(s) 556, 1072
PstNI CAGNNNCTG 1 cut(s) 1076
PsuI RGATCY 1 cut(s) 1063
PvuII CAGCTG 1 cut(s) 1073
RsaI GTAC 3 cut(s) 300, 839, 1169
RsaNI GTAC 3 cut(s) 299, 838, 1168
SacI GAGCTC 1 cut(s) 20
SalI GTCGAC 1 cut(s) 647
SapI GCTCTTC 1 cut(s) 659
SaqAI TTAA 9 cut(s) 254, 507, 525, 614, 686, 969, 1038, 1242, 1296
SatI GCNGC 8 cut(s) 66, 154, 552, 718, 889, 957, 1071, 1074
Sau3AI GATC 2 cut(s) 1063, 1226
Sau96I GGNCC 1 cut(s) 141
SchI GAGTC 1 cut(s) 317
SduI GDGCHC 1 cut(s) 20
SfaNI GCATC 1 cut(s) 1009
SfcI CTRYAG 3 cut(s) 552, 1068, 1248
SfuI TTCGAA 2 cut(s) 562, 1091
Sse9I AATT 9 cut(s) 376, 558, 581, 736, 773, 903, 1009, 1025, 1293
SsiI CCGC 2 cut(s) 153, 1109
SspI AATATT 1 cut(s) 815
SspMI CTAG 2 cut(s) 405, 1321
SstI GAGCTC 1 cut(s) 20
StyI CCWWGG 1 cut(s) 137
TaaI ACNGT 5 cut(s) 437, 448, 612, 966, 1087
TaiI ACGT 2 cut(s) 354, 1173
TaqI TCGA 6 cut(s) 396, 439, 450, 562, 648, 1091
TasI AATT 9 cut(s) 376, 558, 581, 736, 773, 903, 1009, 1025, 1293
TatI WGTACW 1 cut(s) 837
TauI GCSGC 1 cut(s) 156
TfiI GAWTC 4 cut(s) 426, 594, 1185, 1212
Tru1I TTAA 9 cut(s) 254, 507, 525, 614, 686, 969, 1038, 1242, 1296
Tru9I TTAA 9 cut(s) 254, 507, 525, 614, 686, 969, 1038, 1242, 1296
TscAI CASTG 4 cut(s) 75, 333, 787, 1222
TseFI GTSAC 2 cut(s) 106, 1274
TseI GCWGC 7 cut(s) 65, 551, 717, 888, 956, 1070, 1073
Tsp45I GTSAC 2 cut(s) 106, 1274
TspDTI ATGAA 5 cut(s) 230, 272, 301, 1018, 1131
TspGWI ACGGA 2 cut(s) 158, 1197
TspRI CASTG 4 cut(s) 75, 333, 787, 1222
VspI ATTAAT 1 cut(s) 1296
XapI RAATTY 3 cut(s) 558, 773, 1025
XceI RCATGY 1 cut(s) 936
XmiI GTMKAC 3 cut(s) 648, 939, 1251
XspI CTAG 2 cut(s) 405, 1321
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.