Rh5CG104800

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0001843
Physical Location & Seq
Reverse (-)
8571855 .. 8574335
2481 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG104800.1

Sequence Viewer

Length: 1110 bp
ATGGCTTGTGTAATGAAGCTTTTGGTGGTGGTGATTTCTCTGTCTTGGGTACCAAGTATGCAACATTCCTCCTCTGTTCTTGGAGAGCCAGAAGTGCCCTGTTTCTTCATTTTCGGGGACTCACTGGCTGATTGTGGCAACAACAATGTGCTTAATACTACGGCTAAGGTCAACTACGAGCCATATGGTATTGACTTTCCTTATGGACCAACTGGAAGGTTTTGCAACGGCCGCACCACCGTTGATATACTTACTGAACTTCTGGGATTTGAAAACTACATTCCACCATTTGCTTATGCTAATGGCTCTGCCATACTCAGAGGTGTAAACTACGCATCCGGTGGATCAGGAATTCGAAGAGAAACCGGGATGACAACGGGCACAGTACTCAGCATGGGAAAACAGTTAAAGAACCACAGGAAAACAATCATGCGCATCGTCAGCATGCTAGGAAAGAAAAGCTTGGCCAGAAAGTACTTGAACAAGTGCTTATATTCAGTCGGAATGGGCAGCAATGACTACATTAACAATTACTTTTTACCTCAGTACTATAACACCAGTCAAGAATATACAGTTGAGGAATATGCAGTAGTTCTTGTCGAACAATATTCTCGACAAATACTGAGATTGTATGAGTATGGAGCAAAGAAGGTTGTTCTGGGTGGACTGGGACTACTAGGCTGCACTCCAGCTGCAATTGCTAGTTATGGCACGAATGGATCATGTTCCGAAGAACTCAACTATGCAGCCCAACTTTTCAACCAACAGCTTTTATCTCTTGTTGATCAGCTCAACAGCAATTTGACTGATGCAAAGTTCGTCTACATTAACAACTATGAAATCGGTTCAGCCAATACTGCAGCTCTTGGATTCACTGTTTCGGATGCAGCGTGCTGTACACTTAACAGTGGTGGCCTGTGCGAGGTAGACACGACTCCATGCCAGAATAGGGCTGACTACGTGTTTTGGGACGGGTACCATCCTACTGAAGCTTTTAATATGTACAGTGCCAGCAAAAGCTACAGTTCGGATGAGTCTGCCGACACTTATCCAATGGATGTTAGTCACCTAGCTGAGCTAGAATTCAATCCTGTGGTGGCGGAGATGTAG

Protein Analysis

369

Amino Acids

40.48

Weight (kDa)

4.95

Isoelectric Point (pI)

28.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 36 - 334 4.6e-32 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 434
Acc65I GGTACC 2 cut(s) 49, 975
AccB1I GGYRCC 2 cut(s) 49, 975
AccI GTMKAC 2 cut(s) 822, 927
AciI CCGC 2 cut(s) 232, 1100
AclWI GGATC 2 cut(s) 352, 727
AcoI YGGCCR 2 cut(s) 229, 465
AcsI RAATTY 2 cut(s) 351, 1082
AcuI CTGAAG 1 cut(s) 1008
AfaI GTAC 7 cut(s) 51, 387, 476, 548, 898, 977, 1004
AfiI CCNNNNNNNGG 2 cut(s) 922, 949
AflIII ACRYGT 1 cut(s) 960
AgsI TTSAA 4 cut(s) 272, 481, 760, 1087
AlwI GGATC 2 cut(s) 352, 727
AoxI GGCC 3 cut(s) 229, 465, 913
ApeKI GCWGC 6 cut(s) 510, 681, 692, 746, 860, 887
ApoI RAATTY 2 cut(s) 351, 1082
Asp718I GGTACC 2 cut(s) 49, 975
AspLEI GCGC 1 cut(s) 435
AspS9I GGNCC 1 cut(s) 206
AsuC2I CCSGG 1 cut(s) 367
AsuHPI GGTGA 2 cut(s) 43, 1058
AsuII TTCGAA 1 cut(s) 355
AvaII GGWCC 1 cut(s) 206
BaeGI GKGCMC 2 cut(s) 99, 383
BalI TGGCCA 1 cut(s) 467
BanI GGYRCC 2 cut(s) 49, 975
BbvI GCAGC 6 cut(s) 522, 668, 679, 758, 872, 899
BccI CCATC 1 cut(s) 987
BceAI ACGGC 2 cut(s) 177, 244
BclI TGATCA 1 cut(s) 784
BcnI CCSGG 1 cut(s) 367
BfaI CTAG 5 cut(s) 449, 677, 702, 1070, 1079
BfmI CTRYAG 2 cut(s) 858, 1021
BisI GCNGC 7 cut(s) 232, 511, 682, 693, 747, 861, 888
BlpI GCTNAGC 1 cut(s) 1074
BlsI GCNGC 7 cut(s) 233, 512, 683, 694, 748, 862, 889
BmcAI AGTACT 3 cut(s) 387, 476, 548
Bme1390I CCNGG 1 cut(s) 367
Bme18I GGWCC 1 cut(s) 206
BmgT120I GGNCC 1 cut(s) 206
BmiI GGNNCC 2 cut(s) 51, 977
BmrFI CCNGG 1 cut(s) 367
BmrI ACTGGG 1 cut(s) 677
BmsI GCATC 4 cut(s) 344, 444, 799, 874
BmuI ACTGGG 1 cut(s) 677
BpmI CTGGAG 1 cut(s) 672
Bpu10I CCTNAGC 1 cut(s) 165
Bpu1102I GCTNAGC 1 cut(s) 1074
Bpu14I TTCGAA 1 cut(s) 355
BpuMI CCSGG 1 cut(s) 367
BsaAI YACGTR 1 cut(s) 961
BsaWI WCCGGW 1 cut(s) 338
Bsc4I CCNNNNNNNGG 2 cut(s) 922, 949
Bse1I ACTGG 4 cut(s) 129, 217, 558, 672
Bse3DI GCAATG 1 cut(s) 520
BseGI GGATG 6 cut(s) 335, 375, 889, 979, 1036, 1063
BseLI CCNNNNNNNGG 2 cut(s) 922, 949
BseMI GCAATG 1 cut(s) 520
BseMII CTCAG 5 cut(s) 331, 403, 557, 614, 1065
BseNI ACTGG 4 cut(s) 129, 217, 558, 672
BseRI GAGGAG 1 cut(s) 61
BseSI GKGCMC 2 cut(s) 99, 383
BseX3I CGGCCG 1 cut(s) 229
BseXI GCAGC 6 cut(s) 522, 668, 679, 758, 872, 899
BsgI GTGCAG 1 cut(s) 667
Bsh1285I CGRYCG 1 cut(s) 232
BshFI GGCC 3 cut(s) 231, 467, 915
BshNI GGYRCC 2 cut(s) 49, 975
BsiEI CGRYCG 1 cut(s) 232
BsiSI CCGG 2 cut(s) 339, 366
BslFI GGGAC 3 cut(s) 131, 684, 983
BslI CCNNNNNNNGG 2 cut(s) 922, 949
BsmFI GGGAC 3 cut(s) 131, 684, 983
BsnI GGCC 3 cut(s) 231, 467, 915
Bsp119I TTCGAA 1 cut(s) 355
Bsp1286I GDGCHC 2 cut(s) 99, 383
Bsp1407I TGTACA 2 cut(s) 896, 1002
Bsp143I GATC 3 cut(s) 344, 719, 784
Bsp1720I GCTNAGC 1 cut(s) 1074
BspACI CCGC 2 cut(s) 232, 1100
BspANI GGCC 3 cut(s) 231, 467, 915
BspCNI CTCAG 5 cut(s) 330, 402, 556, 615, 1066
BspLI GGNNCC 2 cut(s) 51, 977
BspMAI CTGCAG 1 cut(s) 862
BspPI GGATC 2 cut(s) 352, 727
BspT104I TTCGAA 1 cut(s) 355
BspT107I GGYRCC 2 cut(s) 49, 975
BsrDI GCAATG 1 cut(s) 520
BsrGI TGTACA 2 cut(s) 896, 1002
BsrI ACTGG 4 cut(s) 129, 217, 558, 672
BssMI GATC 3 cut(s) 344, 719, 784
Bst4CI ACNGT 8 cut(s) 241, 385, 405, 574, 877, 908, 1007, 1025
Bst6I CTCTTC 1 cut(s) 352
BstAUI TGTACA 2 cut(s) 896, 1002
BstBAI YACGTR 1 cut(s) 961
BstBI TTCGAA 1 cut(s) 355
BstC8I GCNNGC 3 cut(s) 446, 892, 1012
BstDEI CTNAG 6 cut(s) 165, 317, 389, 543, 623, 1074
BstENI CCTNNNNNAGG 1 cut(s) 920
BstF5I GGATG 6 cut(s) 335, 375, 889, 979, 1036, 1063
BstHHI GCGC 1 cut(s) 435
BstKTI GATC 3 cut(s) 347, 722, 787
BstMBI GATC 3 cut(s) 344, 719, 784
BstMCI CGRYCG 1 cut(s) 232
BstMWI GCNNNNNNNGC 5 cut(s) 94, 231, 441, 698, 857
BstNSI RCATGY 1 cut(s) 448
BstSCI CCNGG 1 cut(s) 365
BstSFI CTRYAG 2 cut(s) 858, 1021
BstSLI GKGCMC 2 cut(s) 99, 383
BstV1I GCAGC 6 cut(s) 522, 668, 679, 758, 872, 899
BstZI CGGCCG 1 cut(s) 229
BsuRI GGCC 3 cut(s) 231, 467, 915
BtsCI GGATG 6 cut(s) 335, 375, 889, 979, 1036, 1063
BtsIMutI CAGTG 4 cut(s) 122, 873, 913, 1012
Cac8I GCNNGC 3 cut(s) 446, 892, 1012
CfoI GCGC 1 cut(s) 435
Cfr13I GGNCC 1 cut(s) 206
Csp6I GTAC 7 cut(s) 50, 386, 475, 547, 897, 976, 1003
CviAII CATG 5 cut(s) 394, 430, 445, 723, 939
CviQI GTAC 7 cut(s) 50, 386, 475, 547, 897, 976, 1003
DdeI CTNAG 6 cut(s) 165, 317, 389, 543, 623, 1074
DpnI GATC 3 cut(s) 346, 721, 786
DpnII GATC 3 cut(s) 344, 719, 784
EaeI YGGCCR 2 cut(s) 229, 465
EagI CGGCCG 1 cut(s) 229
Eam1104I CTCTTC 1 cut(s) 352
EarI CTCTTC 1 cut(s) 352
EclXI CGGCCG 1 cut(s) 229
Eco47I GGWCC 1 cut(s) 206
Eco52I CGGCCG 1 cut(s) 229
Eco57I CTGAAG 1 cut(s) 1008
EcoNI CCTNNNNNAGG 1 cut(s) 920
EcoRI GAATTC 2 cut(s) 351, 1082
FaeI CATG 5 cut(s) 397, 433, 448, 726, 942
FalI AAGNNNNNCTT 2 cut(s) 446, 478
FaqI GGGAC 3 cut(s) 131, 684, 983
FatI CATG 5 cut(s) 393, 429, 444, 722, 938
FauNDI CATATG 1 cut(s) 184
FbaI TGATCA 1 cut(s) 784
FblI GTMKAC 2 cut(s) 822, 927
Fnu4HI GCNGC 7 cut(s) 232, 511, 682, 693, 747, 861, 888
FokI GGATG 6 cut(s) 322, 382, 896, 966, 1043, 1070
Fsp4HI GCNGC 7 cut(s) 232, 511, 682, 693, 747, 861, 888
FspAI RTGCGCAY 1 cut(s) 434
FspBI CTAG 5 cut(s) 449, 677, 702, 1070, 1079
FspI TGCGCA 1 cut(s) 434
GlaI GCGC 1 cut(s) 434
GluI GCNGC 7 cut(s) 232, 511, 682, 693, 747, 861, 888
GsuI CTGGAG 1 cut(s) 672
HaeIII GGCC 3 cut(s) 231, 467, 915
HapII CCGG 2 cut(s) 339, 366
HhaI GCGC 1 cut(s) 435
Hin1II CATG 5 cut(s) 397, 433, 448, 726, 942
Hin6I GCGC 1 cut(s) 433
HinP1I GCGC 1 cut(s) 433
HincII GTYRAC 1 cut(s) 172
HindII GTYRAC 1 cut(s) 172
HindIII AAGCTT 3 cut(s) 17, 460, 990
HinfI GANTC 4 cut(s) 119, 870, 934, 1034
HpaII CCGG 2 cut(s) 339, 366
HphI GGTGA 2 cut(s) 43, 1058
Hpy166II GTNNAC 6 cut(s) 172, 328, 665, 823, 899, 928
Hpy188I TCNGA 5 cut(s) 320, 503, 730, 883, 1030
Hpy188III TCNNGA 3 cut(s) 348, 563, 612
Hpy8I GTNNAC 6 cut(s) 172, 328, 665, 823, 899, 928
HpyAV CCTTC 2 cut(s) 210, 643
HpyCH4III ACNGT 8 cut(s) 241, 385, 405, 574, 877, 908, 1007, 1025
HpyCH4IV ACGT 1 cut(s) 960
HpyCH4V TGCA 9 cut(s) 61, 225, 587, 684, 695, 746, 812, 860, 887
HpyF10VI GCNNNNNNNGC 5 cut(s) 94, 231, 441, 698, 857
HpyF3I CTNAG 6 cut(s) 165, 317, 389, 543, 623, 1074
HpySE526I ACGT 1 cut(s) 960
Hsp92II CATG 5 cut(s) 397, 433, 448, 726, 942
HspAI GCGC 1 cut(s) 433
KpnI GGTACC 2 cut(s) 53, 979
Ksp22I TGATCA 1 cut(s) 784
Kzo9I GATC 3 cut(s) 344, 719, 784
LmnI GCTCC 1 cut(s) 641
Lsp1109I GCAGC 6 cut(s) 522, 668, 679, 758, 872, 899
LweI GCATC 4 cut(s) 344, 444, 799, 874
MaeI CTAG 5 cut(s) 449, 677, 702, 1070, 1079
MaeII ACGT 1 cut(s) 960
MaeIII GTNAC 1 cut(s) 1064
MalI GATC 3 cut(s) 346, 721, 786
MboI GATC 3 cut(s) 344, 719, 784
MboII GAAGA 3 cut(s) 97, 369, 743
MfeI CAATTG 1 cut(s) 696
MhlI GDGCHC 2 cut(s) 99, 383
MlsI TGGCCA 1 cut(s) 467
MluCI AATT 5 cut(s) 351, 529, 696, 799, 1082
MluNI TGGCCA 1 cut(s) 467
MlyI GAGTC 3 cut(s) 113, 928, 1043
MmeI TCCRAC 1 cut(s) 481
MnlI CCTC 6 cut(s) 79, 82, 314, 552, 571, 916
Mox20I TGGCCA 1 cut(s) 467
MscI TGGCCA 1 cut(s) 467
MseI TTAA 6 cut(s) 153, 407, 525, 828, 903, 996
Msp20I TGGCCA 1 cut(s) 467
MspA1I CMGCKG 1 cut(s) 692
MspI CCGG 2 cut(s) 339, 366
MspR9I CCNGG 1 cut(s) 367
MunI CAATTG 1 cut(s) 696
MwoI GCNNNNNNNGC 5 cut(s) 94, 231, 441, 698, 857
NciI CCSGG 1 cut(s) 367
NdeI CATATG 1 cut(s) 184
NdeII GATC 3 cut(s) 344, 719, 784
NlaIII CATG 5 cut(s) 397, 433, 448, 726, 942
NlaIV GGNNCC 2 cut(s) 51, 977
NmuCI GTSAC 1 cut(s) 1064
NsbI TGCGCA 1 cut(s) 434
NspI RCATGY 1 cut(s) 448
NspV TTCGAA 1 cut(s) 355
PaeI GCATGC 1 cut(s) 448
PcsI WCGNNNNNNNCGW 1 cut(s) 887
PfeI GAWTC 1 cut(s) 870
PkrI GCNGC 7 cut(s) 233, 512, 683, 694, 748, 862, 889
PleI GAGTC 3 cut(s) 113, 928, 1042
PpsI GAGTC 3 cut(s) 113, 928, 1042
Ppu21I YACGTR 1 cut(s) 961
PspN4I GGNNCC 2 cut(s) 51, 977
PspPI GGNCC 1 cut(s) 206
PstI CTGCAG 1 cut(s) 862
PvuII CAGCTG 1 cut(s) 692
RsaI GTAC 7 cut(s) 51, 387, 476, 548, 898, 977, 1004
RsaNI GTAC 7 cut(s) 50, 386, 475, 547, 897, 976, 1003
SaqAI TTAA 6 cut(s) 153, 407, 525, 828, 903, 996
SatI GCNGC 7 cut(s) 232, 511, 682, 693, 747, 861, 888
Sau3AI GATC 3 cut(s) 344, 719, 784
Sau96I GGNCC 1 cut(s) 206
ScaI AGTACT 3 cut(s) 387, 476, 548
SchI GAGTC 3 cut(s) 113, 928, 1043
ScrFI CCNGG 1 cut(s) 367
SduI GDGCHC 2 cut(s) 99, 383
SfaNI GCATC 4 cut(s) 344, 444, 799, 874
SfcI CTRYAG 2 cut(s) 858, 1021
SfuI TTCGAA 1 cut(s) 355
SinI GGWCC 1 cut(s) 206
SphI GCATGC 1 cut(s) 448
Sse9I AATT 5 cut(s) 351, 529, 696, 799, 1082
SsiI CCGC 2 cut(s) 232, 1100
SspI AATATT 1 cut(s) 608
SspMI CTAG 5 cut(s) 449, 677, 702, 1070, 1079
StyD4I CCNGG 1 cut(s) 365
TaaI ACNGT 8 cut(s) 241, 385, 405, 574, 877, 908, 1007, 1025
TaiI ACGT 1 cut(s) 963
TaqI TCGA 3 cut(s) 355, 600, 613
TasI AATT 5 cut(s) 351, 529, 696, 799, 1082
TatI WGTACW 5 cut(s) 385, 474, 546, 896, 1002
TauI GCSGC 1 cut(s) 234
TfiI GAWTC 1 cut(s) 870
Tru1I TTAA 6 cut(s) 153, 407, 525, 828, 903, 996
Tru9I TTAA 6 cut(s) 153, 407, 525, 828, 903, 996
TscAI CASTG 4 cut(s) 129, 880, 913, 1012
TseFI GTSAC 1 cut(s) 1064
TseI GCWGC 6 cut(s) 510, 681, 692, 746, 860, 887
Tsp45I GTSAC 1 cut(s) 1064
TspDTI ATGAA 3 cut(s) 29, 97, 852
TspRI CASTG 4 cut(s) 129, 880, 913, 1012
VpaK11BI GGWCC 1 cut(s) 206
XagI CCTNNNNNAGG 1 cut(s) 920
XapI RAATTY 2 cut(s) 351, 1082
XceI RCATGY 1 cut(s) 448
XmiI GTMKAC 2 cut(s) 822, 927
XspI CTAG 5 cut(s) 449, 677, 702, 1070, 1079
ZrmI AGTACT 3 cut(s) 387, 476, 548
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.