Rh5BG092100

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0001843
Physical Location & Seq
Reverse (-)
8205016 .. 8207714
2699 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG092100.1

Sequence Viewer

Length: 936 bp
ATGTGGGATGTATATATTCTCAACAAGAAAAACCATCTTCTCCCCCATTCCAGGCTGATTGATCATCTTCTTCTTACAGCTGAACTTCTGGGATTTGAAAACTACATTCCACCATTTGCTTATGCTAATGGCTCTGCCATACTCAGCGGTGTAAACTACGCATCCGGTGGATCAGGAATTCGAAGAGAAACCGGAATAACAACGGGCACAGTACTCAGCATGGGAAAACAGTTAAAAAACCACAGGAAAACAATCATGCGCATCATCAGCATGCTAGGAAAGAAAAGCTTGGCCAGAAAGTACTTGAACAAGTGCTTATATTCAGTCGGAATGGGCAGCAATGACTACATTAACAATTACTTTTTACCTCAGTACTATAACACCAGTCAAGAATATACAGTTGAGGAATATGCAGTAGTTCTTGTCGAACAATATTCTCGGCAAATACTGAGATTGTATGAGTATGGAGCAAAGAAGGTTGTTCTGAGTGGACTGGGACTACTAGGCTGCACTCCTGCTGCAATTGCTAGTTATGGCACGAATGGATCATGTTCCGAAGAACTCAACTATGCAGCCCAACTTTTCAACCAACAGCTTGTATCTCTTGTTGATCAGCTCAACAGCAATTTGACTGATGCAAAGTTCGTCTACATTAACAACTATGAAATCGGTTCAGCCAATACTACAGCTCTTGGATTCACTGTTTCGGATGCTGGGTGCTGTATACTTAACAGTGGTGGTCTGTGTGAGGCAGACACGACTCCATGCCAGAATAGGGCTGACTACGTGTTTTGGGACGGGTTCCATCCTACTGAAGCTTTTAATATGTTCAGTGCCAACAAAAGCTACAGTTCGGATGAGTCGGCCGACACTTATCCAATGGATATTAGTCACCTAGTTGAGCTAGAATTCAATCCTCTGGTGGCGGAGATGTAG

Protein Analysis

311

Amino Acids

34.48

Weight (kDa)

5.4

Isoelectric Point (pI)

27.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 27 - 278 9.4e-26 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 260
AccI GTMKAC 2 cut(s) 648, 724
AciI CCGC 2 cut(s) 147, 926
AclWI GGATC 2 cut(s) 178, 553
AcoI YGGCCR 2 cut(s) 291, 864
AcsI RAATTY 2 cut(s) 177, 908
AcuI CTGAAG 1 cut(s) 834
AfaI GTAC 3 cut(s) 213, 302, 374
AfiI CCNNNNNNNGG 2 cut(s) 51, 775
AflIII ACRYGT 1 cut(s) 786
AgsI TTSAA 4 cut(s) 98, 307, 586, 913
AjnI CCWGG 1 cut(s) 50
AluBI AGCT 8 cut(s) 80, 288, 595, 616, 689, 818, 846, 904
AluI AGCT 8 cut(s) 80, 288, 595, 616, 689, 818, 846, 904
AlwI GGATC 2 cut(s) 178, 553
AoxI GGCC 2 cut(s) 291, 864
ApeKI GCWGC 4 cut(s) 336, 507, 518, 572
ApoI RAATTY 2 cut(s) 177, 908
AspLEI GCGC 1 cut(s) 261
AsuHPI GGTGA 1 cut(s) 884
AsuII TTCGAA 1 cut(s) 181
BaeGI GKGCMC 1 cut(s) 209
BalI TGGCCA 1 cut(s) 293
BbvI GCAGC 4 cut(s) 348, 494, 505, 584
BccI CCATC 2 cut(s) 42, 813
BciT130I CCWGG 1 cut(s) 52
BclI TGATCA 2 cut(s) 61, 610
BfaI CTAG 5 cut(s) 275, 503, 528, 896, 905
BfmI CTRYAG 2 cut(s) 684, 847
BisI GCNGC 4 cut(s) 337, 508, 519, 573
BlsI GCNGC 4 cut(s) 338, 509, 520, 574
BmcAI AGTACT 3 cut(s) 213, 302, 374
Bme1390I CCNGG 1 cut(s) 52
BmiI GGNNCC 1 cut(s) 803
BmrFI CCNGG 1 cut(s) 52
BmrI ACTGGG 1 cut(s) 503
BmsI GCATC 4 cut(s) 170, 270, 625, 700
BmuI ACTGGG 1 cut(s) 503
Bpu14I TTCGAA 1 cut(s) 181
BsaAI YACGTR 1 cut(s) 787
BsaWI WCCGGW 2 cut(s) 164, 191
Bsc4I CCNNNNNNNGG 2 cut(s) 51, 775
Bse1I ACTGG 2 cut(s) 384, 498
Bse3DI GCAATG 1 cut(s) 346
BseBI CCWGG 1 cut(s) 52
BseGI GGATG 5 cut(s) 13, 161, 715, 805, 862
BseLI CCNNNNNNNGG 2 cut(s) 51, 775
BseMI GCAATG 1 cut(s) 346
BseMII CTCAG 5 cut(s) 157, 229, 383, 440, 476
BseNI ACTGG 2 cut(s) 384, 498
BseSI GKGCMC 1 cut(s) 209
BseX3I CGGCCG 1 cut(s) 864
BseXI GCAGC 4 cut(s) 348, 494, 505, 584
BseYI CCCAGC 1 cut(s) 713
BsgI GTGCAG 1 cut(s) 493
Bsh1285I CGRYCG 1 cut(s) 867
BshFI GGCC 2 cut(s) 293, 866
BsiEI CGRYCG 1 cut(s) 867
BsiSI CCGG 2 cut(s) 165, 192
BslFI GGGAC 2 cut(s) 510, 809
BslI CCNNNNNNNGG 2 cut(s) 51, 775
BsmFI GGGAC 2 cut(s) 510, 809
BsnI GGCC 2 cut(s) 293, 866
Bsp119I TTCGAA 1 cut(s) 181
Bsp1286I GDGCHC 1 cut(s) 209
Bsp143I GATC 4 cut(s) 61, 170, 545, 610
BspACI CCGC 2 cut(s) 147, 926
BspANI GGCC 2 cut(s) 293, 866
BspCNI CTCAG 5 cut(s) 156, 228, 382, 441, 477
BspLI GGNNCC 1 cut(s) 803
BspPI GGATC 2 cut(s) 178, 553
BspT104I TTCGAA 1 cut(s) 181
BsrDI GCAATG 1 cut(s) 346
BsrI ACTGG 2 cut(s) 384, 498
BssMI GATC 4 cut(s) 61, 170, 545, 610
BssNAI GTATAC 1 cut(s) 725
Bst1107I GTATAC 1 cut(s) 725
Bst2UI CCWGG 1 cut(s) 52
Bst4CI ACNGT 6 cut(s) 211, 231, 400, 703, 734, 851
Bst6I CTCTTC 1 cut(s) 178
BstBAI YACGTR 1 cut(s) 787
BstBI TTCGAA 1 cut(s) 181
BstC8I GCNNGC 1 cut(s) 272
BstDEI CTNAG 5 cut(s) 143, 215, 369, 449, 485
BstF5I GGATG 5 cut(s) 13, 161, 715, 805, 862
BstHHI GCGC 1 cut(s) 261
BstKTI GATC 4 cut(s) 64, 173, 548, 613
BstMBI GATC 4 cut(s) 61, 170, 545, 610
BstMCI CGRYCG 1 cut(s) 867
BstMWI GCNNNNNNNGC 2 cut(s) 267, 524
BstNI CCWGG 1 cut(s) 52
BstNSI RCATGY 1 cut(s) 274
BstSCI CCNGG 1 cut(s) 50
BstSFI CTRYAG 2 cut(s) 684, 847
BstSLI GKGCMC 1 cut(s) 209
BstV1I GCAGC 4 cut(s) 348, 494, 505, 584
BstZ17I GTATAC 1 cut(s) 725
BstZI CGGCCG 1 cut(s) 864
BsuRI GGCC 2 cut(s) 293, 866
BtsCI GGATG 5 cut(s) 13, 161, 715, 805, 862
BtsIMutI CAGTG 3 cut(s) 699, 739, 838
Cac8I GCNNGC 1 cut(s) 272
CfoI GCGC 1 cut(s) 261
Csp6I GTAC 3 cut(s) 212, 301, 373
CviAII CATG 5 cut(s) 220, 256, 271, 549, 765
CviQI GTAC 3 cut(s) 212, 301, 373
DdeI CTNAG 5 cut(s) 143, 215, 369, 449, 485
DpnI GATC 4 cut(s) 63, 172, 547, 612
DpnII GATC 4 cut(s) 61, 170, 545, 610
EaeI YGGCCR 2 cut(s) 291, 864
EagI CGGCCG 1 cut(s) 864
Eam1104I CTCTTC 1 cut(s) 178
EarI CTCTTC 1 cut(s) 178
EclXI CGGCCG 1 cut(s) 864
Eco52I CGGCCG 1 cut(s) 864
Eco57I CTGAAG 1 cut(s) 834
EcoRI GAATTC 2 cut(s) 177, 908
EcoRII CCWGG 1 cut(s) 50
FaeI CATG 5 cut(s) 223, 259, 274, 552, 768
FalI AAGNNNNNCTT 2 cut(s) 272, 304
FaqI GGGAC 2 cut(s) 510, 809
FatI CATG 5 cut(s) 219, 255, 270, 548, 764
FbaI TGATCA 2 cut(s) 61, 610
FblI GTMKAC 2 cut(s) 648, 724
Fnu4HI GCNGC 4 cut(s) 337, 508, 519, 573
FokI GGATG 5 cut(s) 20, 148, 722, 792, 869
Fsp4HI GCNGC 4 cut(s) 337, 508, 519, 573
FspAI RTGCGCAY 1 cut(s) 260
FspBI CTAG 5 cut(s) 275, 503, 528, 896, 905
FspI TGCGCA 1 cut(s) 260
GlaI GCGC 1 cut(s) 260
GluI GCNGC 4 cut(s) 337, 508, 519, 573
GsaI CCCAGC 1 cut(s) 717
HaeIII GGCC 2 cut(s) 293, 866
HapII CCGG 2 cut(s) 165, 192
HhaI GCGC 1 cut(s) 261
Hin1II CATG 5 cut(s) 223, 259, 274, 552, 768
Hin6I GCGC 1 cut(s) 259
HinP1I GCGC 1 cut(s) 259
HindIII AAGCTT 2 cut(s) 286, 816
HinfI GANTC 3 cut(s) 696, 760, 860
HpaII CCGG 2 cut(s) 165, 192
HphI GGTGA 1 cut(s) 884
Hpy166II GTNNAC 4 cut(s) 154, 491, 649, 725
Hpy188I TCNGA 5 cut(s) 329, 486, 556, 709, 856
Hpy188III TCNNGA 2 cut(s) 174, 389
Hpy8I GTNNAC 4 cut(s) 154, 491, 649, 725
HpyAV CCTTC 1 cut(s) 469
HpyCH4III ACNGT 6 cut(s) 211, 231, 400, 703, 734, 851
HpyCH4IV ACGT 1 cut(s) 786
HpyCH4V TGCA 5 cut(s) 413, 510, 521, 572, 638
HpyF10VI GCNNNNNNNGC 2 cut(s) 267, 524
HpyF3I CTNAG 5 cut(s) 143, 215, 369, 449, 485
HpySE526I ACGT 1 cut(s) 786
Hsp92II CATG 5 cut(s) 223, 259, 274, 552, 768
HspAI GCGC 1 cut(s) 259
Ksp22I TGATCA 2 cut(s) 61, 610
Kzo9I GATC 4 cut(s) 61, 170, 545, 610
LmnI GCTCC 1 cut(s) 467
Lsp1109I GCAGC 4 cut(s) 348, 494, 505, 584
LweI GCATC 4 cut(s) 170, 270, 625, 700
MaeI CTAG 5 cut(s) 275, 503, 528, 896, 905
MaeII ACGT 1 cut(s) 786
MaeIII GTNAC 1 cut(s) 890
MalI GATC 4 cut(s) 63, 172, 547, 612
MboI GATC 4 cut(s) 61, 170, 545, 610
MboII GAAGA 5 cut(s) 29, 59, 62, 195, 569
MfeI CAATTG 1 cut(s) 522
MhlI GDGCHC 1 cut(s) 209
MlsI TGGCCA 1 cut(s) 293
MluCI AATT 5 cut(s) 177, 355, 522, 625, 908
MluNI TGGCCA 1 cut(s) 293
MlyI GAGTC 2 cut(s) 754, 869
MmeI TCCRAC 1 cut(s) 307
MnlI CCTC 4 cut(s) 378, 397, 742, 927
Mox20I TGGCCA 1 cut(s) 293
MscI TGGCCA 1 cut(s) 293
MseI TTAA 5 cut(s) 233, 351, 654, 729, 822
MslI CAYNNNNRTG 1 cut(s) 269
Msp20I TGGCCA 1 cut(s) 293
MspA1I CMGCKG 2 cut(s) 80, 147
MspI CCGG 2 cut(s) 165, 192
MspR9I CCNGG 1 cut(s) 52
MunI CAATTG 1 cut(s) 522
MvaI CCWGG 1 cut(s) 52
MwoI GCNNNNNNNGC 2 cut(s) 267, 524
NdeII GATC 4 cut(s) 61, 170, 545, 610
NlaIII CATG 5 cut(s) 223, 259, 274, 552, 768
NlaIV GGNNCC 1 cut(s) 803
NmeAIII GCCGAG 1 cut(s) 418
NmuCI GTSAC 1 cut(s) 890
NsbI TGCGCA 1 cut(s) 260
NspI RCATGY 1 cut(s) 274
NspV TTCGAA 1 cut(s) 181
PaeI GCATGC 1 cut(s) 274
PfeI GAWTC 1 cut(s) 696
PkrI GCNGC 4 cut(s) 338, 509, 520, 574
PleI GAGTC 2 cut(s) 754, 868
PpsI GAGTC 2 cut(s) 754, 868
Ppu21I YACGTR 1 cut(s) 787
Psp6I CCWGG 1 cut(s) 50
PspFI CCCAGC 1 cut(s) 713
PspGI CCWGG 1 cut(s) 50
PspN4I GGNNCC 1 cut(s) 803
PvuII CAGCTG 1 cut(s) 80
RsaI GTAC 3 cut(s) 213, 302, 374
RsaNI GTAC 3 cut(s) 212, 301, 373
RseI CAYNNNNRTG 1 cut(s) 269
SaqAI TTAA 5 cut(s) 233, 351, 654, 729, 822
SatI GCNGC 4 cut(s) 337, 508, 519, 573
Sau3AI GATC 4 cut(s) 61, 170, 545, 610
ScaI AGTACT 3 cut(s) 213, 302, 374
SchI GAGTC 2 cut(s) 754, 869
ScrFI CCNGG 1 cut(s) 52
SduI GDGCHC 1 cut(s) 209
SfaNI GCATC 4 cut(s) 170, 270, 625, 700
SfcI CTRYAG 2 cut(s) 684, 847
SfuI TTCGAA 1 cut(s) 181
SmiMI CAYNNNNRTG 1 cut(s) 269
SphI GCATGC 1 cut(s) 274
Sse9I AATT 5 cut(s) 177, 355, 522, 625, 908
SsiI CCGC 2 cut(s) 147, 926
SspI AATATT 1 cut(s) 434
SspMI CTAG 5 cut(s) 275, 503, 528, 896, 905
StyD4I CCNGG 1 cut(s) 50
TaaI ACNGT 6 cut(s) 211, 231, 400, 703, 734, 851
TaiI ACGT 1 cut(s) 789
TaqI TCGA 2 cut(s) 181, 426
TasI AATT 5 cut(s) 177, 355, 522, 625, 908
TatI WGTACW 3 cut(s) 211, 300, 372
TfiI GAWTC 1 cut(s) 696
Tru1I TTAA 5 cut(s) 233, 351, 654, 729, 822
Tru9I TTAA 5 cut(s) 233, 351, 654, 729, 822
TscAI CASTG 3 cut(s) 706, 739, 838
TseFI GTSAC 1 cut(s) 890
TseI GCWGC 4 cut(s) 336, 507, 518, 572
Tsp45I GTSAC 1 cut(s) 890
TspDTI ATGAA 1 cut(s) 678
TspRI CASTG 3 cut(s) 706, 739, 838
XapI RAATTY 2 cut(s) 177, 908
XceI RCATGY 1 cut(s) 274
XmiI GTMKAC 2 cut(s) 648, 724
XspI CTAG 5 cut(s) 275, 503, 528, 896, 905
ZrmI AGTACT 3 cut(s) 213, 302, 374
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.