FvH4_3g08011
RLK Family

cysteine-rich RLK (RECEPTOR-like protein kinase) 8

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0003077
Physical Location & Seq
Forward (+)
4723368 .. 4725018
1651 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g08011.t1

Sequence Viewer

Length: 684 bp
ATGGAACCTAGTTGTTATTCTCAGGCATCCAAAGATGAACAGTGGCGTGCTGCAATGGCAACTGAAATCAATGCCCTGCTCAAGAACCAAACTTGGACTCTTGTACCACCATCTCCTGTTCAGCATGTTGTTGGATGTAAATGGGTGTTTCGCATCAAGCGAAAACCGGATGGTTCTGTGGAGCGCTACAAGGCGCGCCTTGTCGCTAAGGGATTTCATCAACAACACGGGATCGACTATGCTGAAACTTACAGTCCAGTGATCAAACCTGTAACCATCCGCATTGTCTTGTCTCTTGCCGTCTCCCGTGGCTGGCCTCTACGCCAATTGGATGTCACCAATGCCTTCCTCCATGGATACTTGACCGAAGACGTATATATGAGCCAACCTCCTGGCTTCGTTGATCATACACGACCCTCTCATGTCTGTAAACTCACCAAAGCACTCTACGGGCTCAAACAAGCACTCAGGGCCTGGTTTCAACGGATGAGTAACTTTCTTCTGTCAGTTGGATTCCGCCATAGTAAGGCTGACTCGTCTCTTTTCATCTTTCAAAATGGTAAGCATACAATTTTCTTCCTGCTATATGTTGATGACATAATTCTCACCGGTAGTACTCCTCAGCTTTTACAGCGCTTTATTGACACTTTGAGTCACTCCTTTTGTAATACCCCGGAATTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_2 Retrotran_gag_2 gag_pre-integrs rve

Protein Analysis

228

Amino Acids

25.99

Weight (kDa)

9.49

Isoelectric Point (pI)

36.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_2 PF07727 29 - 221 4.6e-54 Reverse transcriptase (RNA-dependent DNA polymerase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 196
AciI CCGC 2 cut(s) 280, 517
AclWI GGATC 1 cut(s) 239
AcsI RAATTY 1 cut(s) 677
AfaI GTAC 2 cut(s) 105, 616
AfeI AGCGCT 2 cut(s) 185, 635
AfiI CCNNNNNNNGG 2 cut(s) 312, 526
AgeI ACCGGT 1 cut(s) 608
AgsI TTSAA 2 cut(s) 482, 554
AjnI CCWGG 2 cut(s) 391, 473
AluBI AGCT 1 cut(s) 625
AluI AGCT 1 cut(s) 625
Alw26I GTCTC 3 cut(s) 297, 307, 543
AlwI GGATC 1 cut(s) 239
AlwNI CAGNNNCTG 1 cut(s) 474
Aor51HI AGCGCT 2 cut(s) 185, 635
AoxI GGCC 2 cut(s) 314, 471
ApeKI GCWGC 1 cut(s) 50
ApoI RAATTY 1 cut(s) 677
AscI GGCGCGCC 1 cut(s) 194
AsiGI ACCGGT 1 cut(s) 608
AspLEI GCGC 4 cut(s) 186, 196, 198, 636
AspS9I GGNCC 1 cut(s) 471
AsuC2I CCSGG 1 cut(s) 674
AsuHPI GGTGA 3 cut(s) 328, 427, 598
BaeI ACNNNNGTAYC 2 cut(s) 87, 120
BanII GRGCYC 1 cut(s) 456
BbsI GAAGAC 1 cut(s) 375
BbvCI CCTCAGC 1 cut(s) 621
BbvI GCAGC 1 cut(s) 37
BccI CCATC 3 cut(s) 118, 164, 284
BceAI ACGGC 1 cut(s) 284
BciT130I CCWGG 2 cut(s) 393, 475
BciVI GTATCC 1 cut(s) 350
BclI TGATCA 2 cut(s) 261, 403
BcnI CCSGG 1 cut(s) 674
BcoDI GTCTC 3 cut(s) 297, 307, 543
BfaI CTAG 1 cut(s) 9
BfoI RGCGCY 2 cut(s) 187, 637
BfuI GTATCC 1 cut(s) 350
BisI GCNGC 1 cut(s) 51
BlsI GCNGC 1 cut(s) 52
BmcAI AGTACT 1 cut(s) 616
Bme1390I CCNGG 3 cut(s) 393, 475, 674
BmgT120I GGNCC 1 cut(s) 471
BmiI GGNNCC 1 cut(s) 6
BmrFI CCNGG 3 cut(s) 393, 475, 674
BmsI GCATC 2 cut(s) 35, 162
BpiI GAAGAC 1 cut(s) 375
BplI GAGNNNNNCTC 2 cut(s) 373, 405
Bpu10I CCTNAGC 2 cut(s) 207, 621
BpuEI CTTGAG 1 cut(s) 65
BpuMI CCSGG 1 cut(s) 674
BsaJI CCNNGG 3 cut(s) 307, 352, 672
BsaWI WCCGGW 2 cut(s) 166, 608
Bsc4I CCNNNNNNNGG 2 cut(s) 312, 526
Bse118I RCCGGY 1 cut(s) 608
Bse1I ACTGG 1 cut(s) 257
Bse3DI GCAATG 1 cut(s) 60
BseBI CCWGG 2 cut(s) 393, 475
BseDI CCNNGG 3 cut(s) 307, 352, 672
BseGI GGATG 6 cut(s) 26, 140, 175, 276, 337, 492
BseLI CCNNNNNNNGG 2 cut(s) 312, 526
BseMI GCAATG 1 cut(s) 60
BseMII CTCAG 3 cut(s) 35, 481, 635
BseNI ACTGG 1 cut(s) 257
BsePI GCGCGC 1 cut(s) 194
BseRI GAGGAG 1 cut(s) 609
BseXI GCAGC 1 cut(s) 37
Bsh1236I CGCG 1 cut(s) 196
BshFI GGCC 2 cut(s) 316, 473
BshTI ACCGGT 1 cut(s) 608
BsiSI CCGG 3 cut(s) 167, 609, 674
BslI CCNNNNNNNGG 2 cut(s) 312, 526
BsmAI GTCTC 3 cut(s) 297, 307, 543
BsmBI CGTCTC 2 cut(s) 307, 543
BsnI GGCC 2 cut(s) 316, 473
Bsp1286I GDGCHC 1 cut(s) 456
Bsp143I GATC 3 cut(s) 231, 261, 403
Bsp19I CCATGG 1 cut(s) 352
BspACI CCGC 2 cut(s) 280, 517
BspANI GGCC 2 cut(s) 316, 473
BspCNI CTCAG 3 cut(s) 34, 480, 634
BspFNI CGCG 1 cut(s) 196
BspLI GGNNCC 1 cut(s) 6
BspPI GGATC 1 cut(s) 239
BsrDI GCAATG 1 cut(s) 60
BsrFI RCCGGY 1 cut(s) 608
BsrI ACTGG 1 cut(s) 257
BssAI RCCGGY 1 cut(s) 608
BssECI CCNNGG 3 cut(s) 307, 352, 672
BssHII GCGCGC 1 cut(s) 194
BssMI GATC 3 cut(s) 231, 261, 403
BssT1I CCWWGG 1 cut(s) 352
Bst2UI CCWGG 2 cut(s) 393, 475
Bst4CI ACNGT 2 cut(s) 42, 254
BstC8I GCNNGC 3 cut(s) 48, 196, 314
BstDEI CTNAG 4 cut(s) 21, 207, 467, 621
BstDSI CCRYGG 2 cut(s) 307, 352
BstF5I GGATG 6 cut(s) 26, 140, 175, 276, 337, 492
BstFNI CGCG 1 cut(s) 196
BstH2I RGCGCY 2 cut(s) 187, 637
BstHHI GCGC 4 cut(s) 186, 196, 198, 636
BstKTI GATC 3 cut(s) 234, 264, 406
BstMAI GTCTC 3 cut(s) 297, 307, 543
BstMBI GATC 3 cut(s) 231, 261, 403
BstMWI GCNNNNNNNGC 3 cut(s) 56, 470, 631
BstNI CCWGG 2 cut(s) 393, 475
BstNSI RCATGY 1 cut(s) 128
BstSCI CCNGG 3 cut(s) 391, 473, 672
BstUI CGCG 1 cut(s) 196
BstV1I GCAGC 1 cut(s) 37
BstV2I GAAGAC 1 cut(s) 375
BstXI CCANNNNNNTGG 1 cut(s) 392
BsuI GTATCC 1 cut(s) 350
BsuRI GGCC 2 cut(s) 316, 473
BtgI CCRYGG 2 cut(s) 307, 352
BtsCI GGATG 6 cut(s) 26, 140, 175, 276, 337, 492
BtsIMutI CAGTG 2 cut(s) 47, 264
Cac8I GCNNGC 3 cut(s) 48, 196, 314
CaiI CAGNNNCTG 1 cut(s) 474
CfoI GCGC 4 cut(s) 186, 196, 198, 636
Cfr10I RCCGGY 1 cut(s) 608
Cfr13I GGNCC 1 cut(s) 471
Csp6I GTAC 2 cut(s) 104, 615
CspAI ACCGGT 1 cut(s) 608
CviAII CATG 3 cut(s) 125, 353, 422
CviJI RGCY 8 cut(s) 312, 316, 384, 396, 454, 473, 530, 625
CviKI_1 RGCY 8 cut(s) 312, 316, 384, 396, 454, 473, 530, 625
CviQI GTAC 2 cut(s) 104, 615
DdeI CTNAG 4 cut(s) 21, 207, 467, 621
DpnI GATC 3 cut(s) 233, 263, 405
DpnII GATC 3 cut(s) 231, 261, 403
EciI GGCGGA 1 cut(s) 506
Eco130I CCWWGG 1 cut(s) 352
Eco24I GRGCYC 1 cut(s) 456
Eco47III AGCGCT 2 cut(s) 185, 635
EcoO109I RGGNCCY 1 cut(s) 471
EcoRII CCWGG 2 cut(s) 391, 473
EcoT14I CCWWGG 1 cut(s) 352
EcoT38I GRGCYC 1 cut(s) 456
ErhI CCWWGG 1 cut(s) 352
Esp3I CGTCTC 2 cut(s) 307, 543
FaeI CATG 3 cut(s) 128, 356, 425
FatI CATG 3 cut(s) 124, 352, 421
FbaI TGATCA 2 cut(s) 261, 403
Fnu4HI GCNGC 1 cut(s) 51
FokI GGATG 6 cut(s) 13, 147, 182, 263, 344, 499
FriOI GRGCYC 1 cut(s) 456
Fsp4HI GCNGC 1 cut(s) 51
FspBI CTAG 1 cut(s) 9
GlaI GCGC 4 cut(s) 185, 195, 197, 635
GluI GCNGC 1 cut(s) 51
HaeII RGCGCY 2 cut(s) 187, 637
HaeIII GGCC 2 cut(s) 316, 473
HapII CCGG 3 cut(s) 167, 609, 674
HhaI GCGC 4 cut(s) 186, 196, 198, 636
Hin1II CATG 3 cut(s) 128, 356, 425
Hin6I GCGC 4 cut(s) 184, 194, 196, 634
HinP1I GCGC 4 cut(s) 184, 194, 196, 634
HinfI GANTC 4 cut(s) 97, 513, 533, 652
HpaII CCGG 3 cut(s) 167, 609, 674
HphI GGTGA 3 cut(s) 328, 427, 598
Hpy166II GTNNAC 1 cut(s) 431
Hpy188III TCNNGA 1 cut(s) 82
Hpy8I GTNNAC 1 cut(s) 431
HpyAV CCTTC 1 cut(s) 355
HpyCH4III ACNGT 2 cut(s) 42, 254
HpyCH4IV ACGT 1 cut(s) 372
HpyCH4V TGCA 1 cut(s) 53
HpyF10VI GCNNNNNNNGC 3 cut(s) 56, 470, 631
HpyF3I CTNAG 4 cut(s) 21, 207, 467, 621
HpySE526I ACGT 1 cut(s) 372
Hsp92II CATG 3 cut(s) 128, 356, 425
HspAI GCGC 4 cut(s) 184, 194, 196, 634
Ksp22I TGATCA 2 cut(s) 261, 403
Kzo9I GATC 3 cut(s) 231, 261, 403
LmnI GCTCC 1 cut(s) 181
Lsp1109I GCAGC 1 cut(s) 37
LweI GCATC 2 cut(s) 35, 162
MaeI CTAG 1 cut(s) 9
MaeII ACGT 1 cut(s) 372
MaeIII GTNAC 4 cut(s) 271, 334, 491, 653
MalI GATC 3 cut(s) 233, 263, 405
MboI GATC 3 cut(s) 231, 261, 403
MboII GAAGA 3 cut(s) 380, 491, 568
MfeI CAATTG 1 cut(s) 326
MhlI GDGCHC 1 cut(s) 456
MluCI AATT 4 cut(s) 326, 570, 600, 677
MlyI GAGTC 3 cut(s) 91, 527, 661
MmeI TCCRAC 2 cut(s) 112, 490
MnlI CCTC 5 cut(s) 327, 359, 399, 427, 630
MseI TTAA 1 cut(s) 682
MspI CCGG 3 cut(s) 167, 609, 674
MspR9I CCNGG 3 cut(s) 393, 475, 674
MunI CAATTG 1 cut(s) 326
MvaI CCWGG 2 cut(s) 393, 475
MvnI CGCG 1 cut(s) 196
MwoI GCNNNNNNNGC 3 cut(s) 56, 470, 631
NciI CCSGG 1 cut(s) 674
NcoI CCATGG 1 cut(s) 352
NdeII GATC 3 cut(s) 231, 261, 403
NlaIII CATG 3 cut(s) 128, 356, 425
NlaIV GGNNCC 1 cut(s) 6
NmuCI GTSAC 2 cut(s) 334, 653
NspI RCATGY 1 cut(s) 128
PalAI GGCGCGCC 1 cut(s) 194
PauI GCGCGC 1 cut(s) 194
PcsI WCGNNNNNNNCGW 1 cut(s) 157
PfeI GAWTC 1 cut(s) 513
PinAI ACCGGT 1 cut(s) 608
PkrI GCNGC 1 cut(s) 52
PleI GAGTC 3 cut(s) 91, 527, 660
PpsI GAGTC 3 cut(s) 91, 527, 660
Psp6I CCWGG 2 cut(s) 391, 473
PspGI CCWGG 2 cut(s) 391, 473
PspN4I GGNNCC 1 cut(s) 6
PspPI GGNCC 1 cut(s) 471
PstNI CAGNNNCTG 1 cut(s) 474
PteI GCGCGC 1 cut(s) 194
RsaI GTAC 2 cut(s) 105, 616
RsaNI GTAC 2 cut(s) 104, 615
SaqAI TTAA 1 cut(s) 682
SatI GCNGC 1 cut(s) 51
Sau3AI GATC 3 cut(s) 231, 261, 403
Sau96I GGNCC 1 cut(s) 471
ScaI AGTACT 1 cut(s) 616
SchI GAGTC 3 cut(s) 91, 527, 661
ScrFI CCNGG 3 cut(s) 393, 475, 674
SduI GDGCHC 1 cut(s) 456
SetI ASST 5 cut(s) 10, 271, 375, 391, 627
SfaNI GCATC 2 cut(s) 35, 162
SgsI GGCGCGCC 1 cut(s) 194
SmlI CTYRAG 1 cut(s) 80
SmoI CTYRAG 1 cut(s) 80
Sse9I AATT 4 cut(s) 326, 570, 600, 677
SsiI CCGC 2 cut(s) 280, 517
SspMI CTAG 1 cut(s) 9
StyD4I CCNGG 3 cut(s) 391, 473, 672
StyI CCWWGG 1 cut(s) 352
TaaI ACNGT 2 cut(s) 42, 254
TaiI ACGT 1 cut(s) 375
TaqI TCGA 1 cut(s) 234
TaqII GACCGA 1 cut(s) 380
TasI AATT 4 cut(s) 326, 570, 600, 677
TatI WGTACW 1 cut(s) 614
TfiI GAWTC 1 cut(s) 513
Tru1I TTAA 1 cut(s) 682
Tru9I TTAA 1 cut(s) 682
TscAI CASTG 2 cut(s) 47, 264
TseFI GTSAC 2 cut(s) 334, 653
TseI GCWGC 1 cut(s) 50
Tsp45I GTSAC 2 cut(s) 334, 653
TspDTI ATGAA 3 cut(s) 51, 206, 535
TspGWI ACGGA 1 cut(s) 499
TspRI CASTG 2 cut(s) 47, 264
XapI RAATTY 1 cut(s) 677
XceI RCATGY 1 cut(s) 128
XspI CTAG 1 cut(s) 9
ZrmI AGTACT 1 cut(s) 616
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.